##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939047_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3189526 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816331329482814 33.0 31.0 34.0 30.0 34.0 2 31.89875360790287 33.0 31.0 34.0 30.0 34.0 3 31.952077518728487 34.0 31.0 34.0 30.0 34.0 4 35.49217877515343 37.0 35.0 37.0 33.0 37.0 5 35.52925324954241 37.0 35.0 37.0 33.0 37.0 6 35.607629472216246 37.0 35.0 37.0 33.0 37.0 7 35.5280471142107 37.0 35.0 37.0 33.0 37.0 8 35.51616133557149 37.0 35.0 37.0 33.0 37.0 9 37.13257267694322 39.0 37.0 39.0 33.0 39.0 10-11 37.01583981444264 39.0 37.0 39.0 33.0 39.0 12-13 36.90767562327443 39.0 37.0 39.0 33.0 39.0 14-15 38.12716215512901 40.0 38.0 41.0 33.0 41.0 16-17 38.03455670215574 40.0 38.0 41.0 33.0 41.0 18-19 37.93616966909816 40.0 38.0 41.0 33.0 41.0 20-21 38.0408841313725 40.0 38.0 41.0 33.0 41.0 22-23 37.978488182883595 40.0 38.0 41.0 33.0 41.0 24-25 37.89868165363757 40.0 38.0 41.0 32.5 41.0 26-27 37.780229570161836 40.0 38.0 41.0 32.0 41.0 28-29 37.649913027829214 40.0 37.5 41.0 32.0 41.0 30-31 37.46855990513951 40.0 37.0 41.0 31.5 41.0 32-33 37.27421911594387 40.0 37.0 41.0 31.0 41.0 34-35 36.95623174101732 40.0 36.0 41.0 30.5 41.0 36-37 36.69385513709561 39.5 36.0 41.0 30.0 41.0 38-39 36.456576149559524 39.0 35.5 41.0 30.0 41.0 40-41 36.30813465699919 39.0 35.0 41.0 30.0 41.0 42-43 36.09845679263941 39.0 35.0 41.0 29.0 41.0 44-45 36.06083176622482 39.0 35.0 41.0 29.0 41.0 46-47 35.87841077326223 39.0 35.0 41.0 28.0 41.0 48-49 35.648548091471895 39.0 35.0 41.0 27.0 41.0 50-51 34.54150444297993 38.0 34.0 40.0 25.5 40.5 52-53 34.641403926476855 38.0 34.0 39.5 26.0 40.5 54-55 35.13316367384997 39.0 35.0 40.0 26.0 41.0 56-57 35.019676434680264 38.5 34.5 40.0 26.0 41.0 58-59 34.79234579056575 38.0 34.0 40.0 26.0 41.0 60-61 34.47186776342315 38.0 34.0 40.0 25.0 41.0 62-63 34.08288128079219 37.0 34.0 40.0 23.5 41.0 64-65 33.69052235347823 37.0 33.0 40.0 23.0 41.0 66-67 33.27381874297309 36.0 33.0 39.0 22.0 41.0 68-69 32.82395424900126 36.0 32.5 39.0 21.5 40.5 70-71 32.37624477743715 35.0 32.0 38.5 20.0 40.0 72-73 31.93575942005176 35.0 31.5 37.5 18.5 40.0 74-75 31.468372102939433 35.0 31.0 37.0 17.5 39.0 76-77 31.034810971912442 35.0 31.0 36.5 12.5 39.0 78-79 30.637459923512147 35.0 30.5 36.0 7.5 38.5 80-81 30.190957057569058 34.0 30.0 35.5 7.0 37.5 82-83 29.71905449273654 34.0 30.0 35.0 2.0 37.0 84-85 29.300893455641997 34.0 29.0 35.0 2.0 36.5 86-87 28.85739699253118 34.0 29.0 35.0 2.0 36.0 88-89 28.373917158850563 34.0 28.5 35.0 2.0 36.0 90-91 27.908425421206786 34.0 27.0 35.0 2.0 35.0 92-93 27.38708337853336 33.5 26.5 35.0 2.0 35.0 94-95 26.832071129064317 33.0 25.0 35.0 2.0 35.0 96-97 26.160658511640914 33.0 24.5 35.0 2.0 35.0 98-99 25.272402858606576 32.5 20.5 35.0 2.0 35.0 100-101 22.0228074641812 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21475176025299803 1101 2 0.3754870913326336 1101 3 0.3616340641278164 1101 4 0.2289388059135149 1101 5 0.18713638284331324 1101 6 0.3059976466658654 1101 7 0.4111855263471398 1101 8 0.2860476140034436 1101 9 0.4434012878568723 1101 10-11 0.3653804380066319 1101 12-13 0.4029617616524277 1101 14-15 0.4735288244960998 1101 16-17 0.48448099364858166 1101 18-19 0.6264005057588733 1101 20-21 0.5136906245999668 1101 22-23 0.548801799382467 1101 24-25 0.5571768012912415 1101 26-27 0.523544411564167 1101 28-29 0.5723580068621814 1101 30-31 0.5230977746726282 1101 32-33 0.5222830730516961 1101 34-35 0.3516339039682066 1101 36-37 0.3089975682553998 1101 38-39 0.47893143026516327 1101 40-41 0.3906144984220603 1101 42-43 0.2668572745858029 1101 44-45 0.41178875045296337 1101 46-47 0.2881977939995579 1101 48-49 0.2915351226337819 1101 50-51 0.32559336819586093 1101 52-53 0.36250462727651467 1101 54-55 0.44065105178538744 1101 56-57 0.4946781300987837 1101 58-59 0.4411394006827578 1101 60-61 0.40585788400416334 1101 62-63 0.32320536192920457 1101 64-65 0.18847824141492708 1101 66-67 0.16312044978483442 1101 68-69 0.24506234193981413 1101 70-71 0.11801125433732551 1101 72-73 0.09210749150278019 1101 74-75 -0.08625297875526527 1101 76-77 -0.09784262375690034 1101 78-79 -0.11788070560203678 1101 80-81 -0.1308042323469074 1101 82-83 -0.09698378605023095 1101 84-85 -0.0836798959553775 1101 86-87 -0.16238072389114677 1101 88-89 -0.3877221385047136 1101 90-91 -0.1715759319995982 1101 92-93 -0.24997509613925573 1101 94-95 -0.42490169712173653 1101 96-97 -0.5280884856416463 1101 98-99 -0.7918838461005464 1101 100-101 -0.27744599989241436 1102 1 0.28365989164894145 1102 2 0.3182058927524274 1102 3 0.3377453283832672 1102 4 0.18241027228159368 1102 5 0.400193677455448 1102 6 0.4260666468733163 1102 7 0.3640279199836627 1102 8 0.41754200276334785 1102 9 0.44243596699737964 1102 10-11 0.5308140054139727 1102 12-13 0.43365395195431944 1102 14-15 0.6004363477353607 1102 16-17 0.5775754597068996 1102 18-19 0.5242853140616361 1102 20-21 0.5205321031596029 1102 22-23 0.6166340049735268 1102 24-25 0.6007164437292118 1102 26-27 0.6905421920764425 1102 28-29 0.5567415661702455 1102 30-31 0.5106713147413515 1102 32-33 0.5584291215962409 1102 34-35 0.5542742133892347 1102 36-37 0.5227732266418528 1102 38-39 0.5698974615880772 1102 40-41 0.6741083301786333 1102 42-43 0.4946263910250792 1102 44-45 0.7004863004909083 1102 46-47 0.729075187906048 1102 48-49 0.6892885804592552 1102 50-51 0.5453433419087119 1102 52-53 0.6009053456971216 1102 54-55 0.6379518591560256 1102 56-57 0.5936938256566862 1102 58-59 0.5642308827947033 1102 60-61 0.6549551691813349 1102 62-63 0.5829392507502078 1102 64-65 0.5394600734443245 1102 66-67 0.5883599185855459 1102 68-69 0.6751352354868132 1102 70-71 0.5286007968841417 1102 72-73 0.44167561499837404 1102 74-75 0.39865899482686373 1102 76-77 0.4764428063434458 1102 78-79 0.3493968838239958 1102 80-81 0.29436271234778033 1102 82-83 0.3311751758774548 1102 84-85 0.18659522389980054 1102 86-87 0.08956046110500182 1102 88-89 -0.010282023466473333 1102 90-91 0.09742143360203315 1102 92-93 0.08600015699886043 1102 94-95 0.09650263329490727 1102 96-97 -0.053250826533361106 1102 98-99 -0.24571139673527398 1102 100-101 -0.2386812968519152 1103 1 0.1717396726865239 1103 2 0.15862651322133914 1103 3 0.3272943970012925 1103 4 0.21205276184808497 1103 5 0.09731063460169764 1103 6 0.1899156804302109 1103 7 0.09678055805100172 1103 8 0.10995850637690552 1103 9 0.31215638275453017 1103 10-11 0.2650733673013974 1103 12-13 0.2509903804691973 1103 14-15 0.27667762486532865 1103 16-17 0.28903349379536536 1103 18-19 0.3610929981043043 1103 20-21 0.3089893568551645 1103 22-23 0.26094872951745174 1103 24-25 0.2923804902361056 1103 26-27 0.30041139143855133 1103 28-29 0.2664307662109593 1103 30-31 0.3636460254870215 1103 32-33 0.38871266435386076 1103 34-35 0.23248151515247173 1103 36-37 0.3085160136624481 1103 38-39 0.37429480599016784 1103 40-41 0.36119944387848335 1103 42-43 0.3270336769542581 1103 44-45 0.441531687690194 1103 46-47 0.48192543163746393 1103 48-49 0.39492132076937736 1103 50-51 0.2841948071246776 1103 52-53 0.3442907806247959 1103 54-55 0.3823765738602063 1103 56-57 0.3155820371997109 1103 58-59 0.3420470832991356 1103 60-61 0.49748092780242104 1103 62-63 0.4517406690294976 1103 64-65 0.3359857981352832 1103 66-67 0.236468200929437 1103 68-69 0.23894044263023773 1103 70-71 0.25414401821036137 1103 72-73 0.1840111387098773 1103 74-75 0.2449530333036236 1103 76-77 0.21790971881502585 1103 78-79 0.08376544416872278 1103 80-81 0.13169342929135652 1103 82-83 0.0950510142343326 1103 84-85 0.1538056585447194 1103 86-87 0.20191521188822037 1103 88-89 0.10366535090723517 1103 90-91 0.1065384586085365 1103 92-93 0.15867967564346586 1103 94-95 -4.4625662445341163E-4 1103 96-97 -0.08844569856023199 1103 98-99 0.11891220506075939 1103 100-101 0.33850425570020093 1104 1 0.1652563353308203 1104 2 0.2183646012474263 1104 3 0.20511104032929595 1104 4 0.155441526546646 1104 5 0.22497247992770752 1104 6 0.14657357818412464 1104 7 0.18359312889785429 1104 8 0.2266020351289626 1104 9 0.2358310419647225 1104 10-11 0.16652380673589562 1104 12-13 0.28491874557478525 1104 14-15 0.2820581379827871 1104 16-17 0.29489535286137425 1104 18-19 0.4305186194487547 1104 20-21 0.18190783674770472 1104 22-23 0.1918255665169326 1104 24-25 0.21630887095532358 1104 26-27 0.18575676050242151 1104 28-29 0.24824032808800212 1104 30-31 0.3129345680705242 1104 32-33 0.35261617924993516 1104 34-35 0.3607543553300019 1104 36-37 0.3079287180727661 1104 38-39 0.27561018751371336 1104 40-41 0.3302279659679854 1104 42-43 0.28736674470715684 1104 44-45 0.34950304872152316 1104 46-47 0.33212691439710795 1104 48-49 0.3671251536035953 1104 50-51 0.3482496181082908 1104 52-53 0.46690429283978574 1104 54-55 0.4930981868286253 1104 56-57 0.38476949490350165 1104 58-59 0.33971100523811515 1104 60-61 0.3976426850229373 1104 62-63 0.39547176259485184 1104 64-65 0.43261806053707375 1104 66-67 0.5013564675531583 1104 68-69 0.49588198430235053 1104 70-71 0.3627398910368882 1104 72-73 0.42258497178053034 1104 74-75 0.3818865874880579 1104 76-77 0.4748003488964265 1104 78-79 0.2631830757949807 1104 80-81 0.2939793942873763 1104 82-83 0.5188298386706442 1104 84-85 0.5232900317533371 1104 86-87 0.4692898377744612 1104 88-89 0.1802640975341312 1104 90-91 0.24331431511658863 1104 92-93 0.16288135959591088 1104 94-95 0.2550539795874833 1104 96-97 0.1480710943463599 1104 98-99 0.3299403024594838 1104 100-101 0.5935903403412475 1105 1 0.15473285208311438 1105 2 0.20244871488127458 1105 3 0.29790551745889715 1105 4 0.24529487924938564 1105 5 0.18191406873695826 1105 6 0.18675948650917462 1105 7 0.2036696829388447 1105 8 0.2939960687647485 1105 9 0.22743159024668103 1105 10-11 0.2858350685267155 1105 12-13 0.24783418654816103 1105 14-15 0.3699632209846584 1105 16-17 0.38790880398210703 1105 18-19 0.38373991621499925 1105 20-21 0.42628178584517684 1105 22-23 0.20971928596092226 1105 24-25 0.29763723548341403 1105 26-27 0.18451655525921495 1105 28-29 0.29078655916743656 1105 30-31 0.3645708901965321 1105 32-33 0.27729187763615926 1105 34-35 0.27554007911052025 1105 36-37 0.2768342494884948 1105 38-39 0.32642046141300085 1105 40-41 0.32934111860650717 1105 42-43 0.3471977867708773 1105 44-45 0.5101620379733944 1105 46-47 0.37857826257341287 1105 48-49 0.3924538465657008 1105 50-51 0.4049293012924693 1105 52-53 0.4102448940164507 1105 54-55 0.4452180859592403 1105 56-57 0.41055350702923477 1105 58-59 0.2757146559914787 1105 60-61 0.394951284815086 1105 62-63 0.39635634415007104 1105 64-65 0.3457619379401251 1105 66-67 0.3645374286520635 1105 68-69 0.41205794206945257 1105 70-71 0.3010615155466141 1105 72-73 0.2930803331356273 1105 74-75 0.2989377938464699 1105 76-77 0.35013558032803616 1105 78-79 0.428367581478053 1105 80-81 0.3594616269136033 1105 82-83 0.3472144353312352 1105 84-85 0.2827548439978429 1105 86-87 0.29533552894464066 1105 88-89 0.4439058157317035 1105 90-91 0.5454691292427896 1105 92-93 0.6043549364106262 1105 94-95 0.5695727050736181 1105 96-97 0.6275888530884863 1105 98-99 0.46873625732635205 1105 100-101 0.6067115165234398 1106 1 0.20747517426697115 1106 2 0.20815646597882775 1106 3 0.19311562426905127 1106 4 0.21373567105658964 1106 5 0.2035173377901245 1106 6 0.22840231162427926 1106 7 0.2788757659643295 1106 8 0.2608940612224515 1106 9 0.2839979718185788 1106 10-11 0.3284157136205508 1106 12-13 0.32661963935360205 1106 14-15 0.43362058191731023 1106 16-17 0.3908261001493898 1106 18-19 0.44938904010071923 1106 20-21 0.4276737455537969 1106 22-23 0.4775223371775965 1106 24-25 0.5045792365173796 1106 26-27 0.5007130937168895 1106 28-29 0.38904804995875253 1106 30-31 0.40052719156043537 1106 32-33 0.49202253711857225 1106 34-35 0.37885608340032917 1106 36-37 0.4090668664877697 1106 38-39 0.4883781079423457 1106 40-41 0.5404429475463033 1106 42-43 0.35406587899501574 1106 44-45 0.385903269719968 1106 46-47 0.36586846737652934 1106 48-49 0.4229978020213139 1106 50-51 0.2614173821897836 1106 52-53 0.4180561391979509 1106 54-55 0.45379137196082553 1106 56-57 0.4814929980079299 1106 58-59 0.4447935543779522 1106 60-61 0.738032100564233 1106 62-63 0.6007421753251165 1106 64-65 0.3844247782469026 1106 66-67 0.4180484840213552 1106 68-69 0.5174296527718809 1106 70-71 0.47319929545479056 1106 72-73 0.406283993524859 1106 74-75 0.48521518235166283 1106 76-77 0.3645171597597212 1106 78-79 0.4391833849969018 1106 80-81 0.46858148048465154 1106 82-83 0.4238531028143697 1106 84-85 0.590458718542763 1106 86-87 0.5637838182924 1106 88-89 0.6935491858563196 1106 90-91 0.5897417925197743 1106 92-93 0.6746084599409841 1106 94-95 0.6095372104408945 1106 96-97 0.6028883782651668 1106 98-99 0.5561126158765148 1106 100-101 0.7262428083507189 1107 1 0.06925182957353826 1107 2 0.13040010377016742 1107 3 0.15665192550487106 1107 4 0.15470654711337062 1107 5 0.12842263953836408 1107 6 0.13604808005961644 1107 7 0.015548574222364664 1107 8 0.13983763365938273 1107 9 0.1864733367345437 1107 10-11 0.19701714567391093 1107 12-13 0.27838076221866004 1107 14-15 0.28696413892846806 1107 16-17 0.2314000086835719 1107 18-19 0.29724054979578085 1107 20-21 0.29417956980909565 1107 22-23 0.18412786075338516 1107 24-25 0.201907295548871 1107 26-27 0.2581871581088322 1107 28-29 0.22358708598736854 1107 30-31 0.293034880857455 1107 32-33 0.3034139891064669 1107 34-35 0.4096658873001786 1107 36-37 0.3884897661312152 1107 38-39 0.4856575204008706 1107 40-41 0.21615153826646605 1107 42-43 0.3318902007635387 1107 44-45 0.13282539704906782 1107 46-47 0.40511444652137385 1107 48-49 0.5683797724916175 1107 50-51 0.36377149477905135 1107 52-53 0.4067676829691962 1107 54-55 0.5362011588759046 1107 56-57 0.4399127423421376 1107 58-59 0.46151159584620416 1107 60-61 0.45908574861618234 1107 62-63 0.30572373619188653 1107 64-65 0.32457190361701294 1107 66-67 0.29090174757767073 1107 68-69 0.3611968708689375 1107 70-71 0.20187993229310308 1107 72-73 0.23480836235019353 1107 74-75 0.26948875968299646 1107 76-77 0.38726366342190843 1107 78-79 0.4122100244058977 1107 80-81 0.40389210264796205 1107 82-83 0.4070030089263845 1107 84-85 0.3805333202111214 1107 86-87 0.4949801998817378 1107 88-89 0.5105459169050803 1107 90-91 0.5330812530853812 1107 92-93 0.5557687694246773 1107 94-95 0.4923880985921407 1107 96-97 0.6054409643592393 1107 98-99 0.8031571545166472 1107 100-101 0.943833862468523 1108 1 0.26500822885616415 1108 2 0.22211848438482562 1108 3 0.30305844299081386 1108 4 0.2275990126713694 1108 5 0.3077244078327013 1108 6 0.37196006275030413 1108 7 0.426810751201252 1108 8 0.314323805910135 1108 9 0.39659160797717163 1108 10-11 0.4203063395816855 1108 12-13 0.4283805074701448 1108 14-15 0.49582204190664925 1108 16-17 0.5144152035831695 1108 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-2.227307478876739 2316 100-101 -2.1000825990946623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8527.0 3 2390.0 4 2568.0 5 2841.0 6 3340.0 7 4317.0 8 5210.0 9 6675.0 10 7931.0 11 10521.0 12 13531.0 13 18872.0 14 16767.0 15 16869.0 16 20153.0 17 25929.0 18 23252.0 19 18478.0 20 19479.0 21 21227.0 22 23392.0 23 25699.0 24 29568.0 25 33909.0 26 39367.0 27 46418.0 28 55113.0 29 64982.0 30 78799.0 31 95963.0 32 118386.0 33 152373.0 34 204642.0 35 291790.0 36 441441.0 37 639293.0 38 522348.0 39 77154.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48086323802958 18.80886764643902 13.863655092285345 23.846614023246058 2 15.514401045294854 20.3379698837599 39.865202400362485 24.28242667058276 3 19.448384638848754 23.870684493361434 30.03716893475415 26.643761933035666 4 13.023976761067507 16.17165828149023 37.187846886918905 33.616518070523355 5 13.972648487776063 37.037535746199865 34.4588187770068 14.530996989017272 6 32.409537376327464 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22.558761754037437 28-29 22.35733121800174 26.670734370665837 28.634983551372308 22.336950859960112 30-31 22.58333394779502 26.25756905673437 28.381729120291148 22.777367875179454 32-33 22.48937670832319 26.343975031185916 28.492854220577936 22.673794039912956 34-35 22.850349159869694 26.41243246600989 28.291849057256556 22.445369316863857 36-37 22.8425644218481 26.17746476743101 28.550968438776874 22.429002371944012 38-39 22.612043002488182 26.510620054702834 28.393483035852938 22.483853906956043 40-41 22.779587651204217 26.580148627981565 28.196972388302257 22.44329133251196 42-43 22.504137606547207 26.52998506570946 28.032109118454713 22.933768209288623 44-45 22.66005048621018 26.49508370679182 28.103087144794348 22.74177866220365 46-47 22.898843126169517 26.769938513560238 27.778109453833505 22.55310890643674 48-49 22.7461187896617 26.739994435269676 27.785634361926153 22.72825241314247 50-51 22.447974835526136 26.967916272605258 27.922202628777537 22.66190626309107 52-53 22.826205628590536 27.00324462793121 27.462754522904202 22.707795220574045 54-55 22.71390756999501 26.813740633344764 27.5188695756419 22.95348222101833 56-57 22.61270664308609 27.039671976076708 27.485390893127786 22.86223048770942 58-59 22.880613812707065 26.776625066275965 27.57743245598937 22.765328665027596 60-61 22.700993419233118 26.8482759152621 27.942546616848002 22.50818404865678 62-63 22.503564204751928 26.749342880513804 28.003946103522054 22.743146811212213 64-65 22.360796855081635 26.97385741706989 27.779418972834574 22.8859267550139 66-67 22.525657447218528 26.91226825652263 27.469087607578658 23.092986688680188 68-69 22.41725460038105 26.75690667809703 27.780187119721006 23.045651601800913 70-71 22.756652844967686 26.54626613207846 27.547051058342507 23.150029964611345 72-73 22.64517182188521 26.54810183984468 27.707509981069922 23.099216357200188 74-75 22.4262098455993 27.186980857993863 27.332039268190876 23.054770028215955 76-77 22.55422111882648 26.772914014946252 27.37778981991405 23.295075046313215 78-79 22.617287186164354 26.875556268853686 27.403463384417275 23.10369316056469 80-81 22.74043335242347 26.684956656567167 27.29506926317739 23.27954072783197 82-83 22.728839355558435 26.65365849662856 27.610904179658295 23.006597968154715 84-85 22.657056935201574 26.74463205586011 27.419491273787717 23.1788197351506 86-87 22.427322595807905 26.60002759781788 27.679004613498233 23.293645192875985 88-89 22.626812721482644 26.54625309849248 27.35988355103189 23.46705062899299 90-91 22.613723180308583 26.42924520349792 27.415542757495743 23.54148885869775 92-93 22.651328688010697 26.53239947883898 27.334388071469462 23.481883761680862 94-95 22.761609075826282 26.3832382866094 27.339388737502908 23.51576390006141 96-97 22.57953866683011 26.138793856895237 27.689759060348436 23.591908415926216 98-99 22.572936577553605 26.36212329081178 27.269567338379996 23.79537279325462 100-101 22.958816926924364 25.53944430222712 27.785912608550106 23.715826162298406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2369.0 1 1394.0 2 505.0 3 612.0 4 560.0 5 567.5 6 902.0 7 1678.5 8 2689.0 9 3244.0 10 3117.5 11 3022.0 12 3330.0 13 3655.5 14 3997.0 15 4346.5 16 4605.0 17 4816.0 18 5012.0 19 5342.0 20 5887.0 21 6709.5 22 8003.0 23 9870.5 24 11929.5 25 14771.5 26 19672.5 27 25396.5 28 29170.0 29 31992.5 30 35207.0 31 40473.0 32 45681.0 33 49927.5 34 55578.0 35 61350.5 36 68399.5 37 74173.5 38 78048.5 39 81321.5 40 84433.0 41 87793.5 42 89619.0 43 93650.5 44 100510.0 45 105286.5 46 108808.0 47 116343.5 48 128648.5 49 130167.5 50 120992.5 51 117580.5 52 117941.5 53 118609.5 54 117471.0 55 111238.0 56 101973.0 57 92204.5 58 85027.0 59 77309.0 60 65527.0 61 53186.0 62 42208.5 63 32521.0 64 23833.0 65 16941.5 66 11918.0 67 8644.0 68 6418.5 69 4895.5 70 3570.5 71 2145.5 72 1334.0 73 867.0 74 556.5 75 366.0 76 237.0 77 155.5 78 102.0 79 61.0 80 47.5 81 39.0 82 27.5 83 29.5 84 27.5 85 19.0 86 17.0 87 13.0 88 10.0 89 10.0 90 11.0 91 9.5 92 8.0 93 6.0 94 3.5 95 4.0 96 3.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3181663983927392 2 0.08383690868172888 3 0.09386974741701432 4 0.0987294036794182 5 0.11045528395128305 6 0.05558819711769085 7 0.057500707001604624 8 0.05787693845417783 9 0.06091814269581123 10-11 0.058551019806704824 12-13 0.05709312292798365 14-15 0.055164936733545986 16-17 0.05634066002283725 18-19 0.06452369411630443 20-21 0.06642052768969432 22-23 0.08151681472419413 24-25 0.08965281988608965 26-27 0.08451099003425588 28-29 0.0825828038398182 30-31 0.07695500836174404 32-33 0.06866224009460967 34-35 0.3169279698613524 36-37 0.494054602470712 38-39 0.46774975341163544 40-41 0.44991011203545606 42-43 0.4793031942677376 44-45 0.4887873621346871 46-47 0.48319091927766067 48-49 0.5006073002696952 50-51 0.4967979568123916 52-53 0.4959514360441018 54-55 0.4880819281611123 56-57 0.46651132488024866 58-59 0.4500825514512188 60-61 0.4098571386469337 62-63 0.47829991039420905 64-65 0.5024414286009896 66-67 0.5118158622942719 68-69 0.5233065979082786 70-71 0.52097082764022 72-73 0.5100757918261208 74-75 0.5117218044311286 76-77 0.4855266895457193 78-79 0.4676086666169205 80-81 0.47380080927385443 82-83 0.48179572764103507 84-85 0.4822816932672755 86-87 0.48163896453579624 88-89 0.48921062251883196 90-91 0.5029430705377539 92-93 0.5087433054315907 94-95 0.5153587084726696 96-97 0.5072697322423457 98-99 0.5101855259997881 100-101 0.48874033320311544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3189526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.70356968818828 #Duplication Level Percentage of deduplicated Percentage of total 1 70.33001531849911 32.143327562803734 2 14.435530116221265 13.195105133053184 3 5.6324503634105705 7.722692630981892 4 2.861597307852682 5.2314084791590805 5 1.69083867529816 3.8638681613986696 6 1.1031417048290466 3.025050826956069 7 0.7395779003501982 2.3660945075949473 8 0.5308417382954717 1.9409089903668877 9 0.39679104362204487 1.6321290402446171 >10 2.081030763829448 16.907925747374424 >50 0.12899815227824385 4.077747843604772 >100 0.06449819751097761 5.125950280981914 >500 0.003378635031735262 1.0293223282646415 >1k 0.0011032277650619966 0.9964538471765203 >5k 2.0685520594912438E-4 0.742014620038531 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9032 0.28317687330343133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7360 0.2307552909115649 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7154 0.22429665097572493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4495 0.14093003160971254 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3650 0.11443706682434944 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.135262104776697E-5 0.0 0.0 0.0 0.0 3 3.135262104776697E-5 0.0 0.0 0.0 0.0 4 3.135262104776697E-5 0.0 0.0 0.0 0.0 5 3.135262104776697E-5 0.0 0.0 0.0 0.0 6 3.135262104776697E-5 0.0 0.0 0.0 0.0 7 3.135262104776697E-5 0.0 0.0 0.0 0.0 8 3.135262104776697E-5 0.0 0.0 0.0 3.135262104776697E-5 9 3.135262104776697E-5 0.0 0.0 0.0 6.270524209553394E-5 10-11 4.702893157165046E-5 0.0 0.0 0.0 6.270524209553394E-5 12-13 6.270524209553394E-5 1.5676310523883486E-5 0.0 0.0 2.194683473343688E-4 14-15 6.270524209553394E-5 4.702893157165046E-5 0.0 0.0 4.232603841448541E-4 16-17 7.838155261941744E-5 6.270524209553394E-5 0.0 3.135262104776697E-5 4.7028931571650457E-4 18-19 9.405786314330092E-5 6.270524209553394E-5 0.0 3.135262104776697E-5 4.7028931571650457E-4 20-21 9.405786314330092E-5 6.270524209553394E-5 0.0 4.702893157165046E-5 5.643471788598055E-4 22-23 9.405786314330092E-5 6.270524209553394E-5 0.0 9.405786314330092E-5 6.113761104314559E-4 24-25 1.254104841910679E-4 6.270524209553394E-5 0.0 2.821735894299027E-4 7.054339735747569E-4 26-27 1.254104841910679E-4 6.270524209553394E-5 0.0 7.054339735747569E-4 7.211102840986404E-4 28-29 1.5676310523883485E-4 6.270524209553394E-5 0.0 0.0034331120047304837 7.211102840986404E-4 30-31 1.5676310523883485E-4 6.270524209553394E-5 0.0 0.01097341736671844 7.211102840986404E-4 32-33 2.194683473343688E-4 6.270524209553394E-5 0.0 0.02442369179621047 7.524629051464073E-4 34-35 2.9784989995378623E-4 6.270524209553394E-5 0.0 0.04626079235598017 7.524629051464073E-4 36-37 3.448788315254367E-4 6.270524209553394E-5 0.0 0.08242604073457938 7.524629051464073E-4 38-39 3.7623145257320367E-4 6.270524209553394E-5 0.0 0.13439301012125313 7.524629051464073E-4 40-41 3.7623145257320367E-4 7.838155261941744E-5 0.0 0.1934613481752461 7.524629051464073E-4 42-43 3.919077630970872E-4 9.405786314330092E-5 0.0 0.25713852152326083 7.681392156702908E-4 44-45 4.7028931571650457E-4 9.405786314330092E-5 0.0 0.33754231820025926 8.308444577658247E-4 46-47 5.17318247288155E-4 9.405786314330092E-5 0.0 0.43500194072724285 8.935496998613587E-4 48-49 5.329945578120385E-4 9.405786314330092E-5 0.0 0.5347346282801896 9.562549419568926E-4 50-51 5.329945578120385E-4 1.097341736671844E-4 0.0 0.6434341654527977 0.001065989115624077 52-53 5.486708683359221E-4 1.254104841910679E-4 0.0 0.7575263534456217 0.0011130180471957275 54-55 5.80023489383689E-4 1.254104841910679E-4 0.0 0.8874986439991397 0.0011757232892912616 56-57 6.270524209553394E-4 1.254104841910679E-4 0.0 1.0465818431955092 0.0012070759103390283 58-59 6.270524209553394E-4 1.254104841910679E-4 0.0 1.219240727305562 0.0012227522208629119 60-61 6.270524209553394E-4 1.254104841910679E-4 0.0 1.4032022313033348 0.0012227522208629119 62-63 6.270524209553394E-4 1.254104841910679E-4 0.0 1.5919763626319399 0.0012227522208629119 64-65 6.270524209553394E-4 1.254104841910679E-4 0.0 1.8042022545042742 0.0012227522208629119 66-67 6.270524209553394E-4 1.254104841910679E-4 0.0 2.0509317058396763 0.001254104841910679 68-69 6.270524209553394E-4 1.254104841910679E-4 0.0 2.3353783602955422 0.0013011337734823293 70-71 6.427287314792229E-4 1.5676310523883488E-4 0.0 2.6406274788166018 0.0013324863945300964 72-73 7.054339735747569E-4 1.8811572628660183E-4 0.0 2.952868231831313 0.0015519547418644652 74-75 7.524629051464073E-4 1.8811572628660183E-4 0.0 3.284892488727165 0.0017243941576271834 76-77 7.681392156702908E-4 1.8811572628660183E-4 0.0 3.6501191713126024 0.001771423089198834 78-79 7.838155261941743E-4 1.8811572628660183E-4 0.0 4.060430922964729 0.0018184520207704844 80-81 9.092260103852422E-4 1.8811572628660183E-4 0.0 4.509588572095039 0.0018968335733899019 82-83 9.405786314330091E-4 1.8811572628660183E-4 0.0 4.974265768643993 0.001975215126009319 84-85 9.405786314330091E-4 1.8811572628660183E-4 0.0 5.447800080638942 0.0019908914365332027 86-87 9.876075630046595E-4 1.8811572628660183E-4 0.0 5.951119382629269 0.00206927298915262 88-89 0.00103463649457631 1.8811572628660183E-4 0.0 6.505214254406454 0.0021790071628198047 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9555 0.0 22.754726 2 GTACATG 10105 0.0 22.262686 1 ACATGGG 9650 0.0 21.64716 3 GAGTACT 8645 0.0 20.457458 12-13 GTATCAA 18535 0.0 20.305614 1 CATGGGG 6935 0.0 19.739721 4 AGAGTAC 13035 0.0 19.379503 10-11 GGTATCA 14695 0.0 19.184586 1 GTACTTT 9285 0.0 18.818611 14-15 AGTACTT 9170 0.0 18.072773 12-13 CAACGCA 20755 0.0 17.870169 5 ATCAACG 21010 0.0 17.74346 3 TCAACGC 21005 0.0 17.70258 4 TATCAAC 21115 0.0 17.655226 2 AACGCAG 21220 0.0 17.494308 6 GTATAGG 740 0.0 17.3168 1 GAGTACA 8250 0.0 15.99287 1 ACGCAGA 23000 0.0 15.975702 7 GTGTAGC 1635 0.0 15.9654255 1 CGCAGAG 23120 0.0 15.872304 8 >>END_MODULE