FastQCFastQC Report
Fri 27 May 2016
SRR939047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189526
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89330.28007296381970237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78990.24765435365631133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74890.23479977902672683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49220.15431760079710904No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34860.10929523697251567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG91600.021.9155242
GTACATG96750.021.6117151
ACATGGG93500.020.8610973
TATACCG2056.895807E-720.83515
GGTATCA151800.020.802591
GAGTACT94900.019.90495912-13
GTATCAA189700.019.584071
AGAGTAC138250.019.19056110-11
CATGGGG64050.018.5977974
GTACTTT101450.018.45621914-15
GTATTAG7500.018.4167231
TCGTATA1300.0076049418.2529722
AGTACTT100500.017.56795712-13
TTTACGC1650.001473342717.2573573
TATCAAC218200.016.8342272
CAACGCA217950.016.7882125
ATCAACG217700.016.7856923
TCAACGC219750.016.62914
AACGCAG221750.016.5219256
TTTGCGT4802.1827873E-1115.8192443
Produced by FastQC (version 0.11.5)