FastQCFastQC Report
Fri 27 May 2016
SRR939046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194617
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87620.27427388009266834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.2299180152112131No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70540.2208089420421916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44650.13976636322914454No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38080.11920051762073514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG91800.022.5310292
GTACATG96300.022.0568331
GAGTACT85900.020.965112-13
ACATGGG93700.020.8116593
GGTATCA144600.020.7551961
GTATCAA183700.020.441251
CATGGGG67500.019.4938164
GTACTTT93300.018.9992414-15
AGAGTAC130000.018.60370310-11
AGTACAT72200.018.4864772
TATCAAC205350.018.024132
CAACGCA206350.017.891475
ATCAACG207650.017.8019733
TCAACGC208250.017.750964
AGTACTT91400.017.73576212-13
AACGCAG210650.017.5914546
GAGTACG3551.6261765E-917.3622651
GAGTACA81450.017.346691
GTCTACG1952.3092508E-417.0198151
CCTATAC6050.016.429073
Produced by FastQC (version 0.11.5)