##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939046_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3194617 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824016775719905 33.0 31.0 34.0 30.0 34.0 2 31.89077876941117 33.0 31.0 34.0 30.0 34.0 3 31.94659140673201 34.0 31.0 34.0 30.0 34.0 4 35.491243551261384 37.0 35.0 37.0 33.0 37.0 5 35.52012494768543 37.0 35.0 37.0 33.0 37.0 6 35.59567077994013 37.0 35.0 37.0 33.0 37.0 7 35.50432962699441 37.0 35.0 37.0 33.0 37.0 8 35.487263418431695 37.0 35.0 37.0 33.0 37.0 9 37.11079919752509 39.0 37.0 39.0 33.0 39.0 10-11 36.99677989568076 39.0 37.0 39.0 33.0 39.0 12-13 36.875039010936206 39.0 37.0 39.0 33.0 39.0 14-15 38.09736581881333 40.0 38.0 41.0 33.0 41.0 16-17 38.01600317033309 40.0 38.0 41.0 33.0 41.0 18-19 37.94425122009931 40.0 38.0 41.0 33.0 41.0 20-21 37.812437766405175 40.0 38.0 41.0 32.0 41.0 22-23 37.898289998456775 40.0 38.0 41.0 32.5 41.0 24-25 37.83149670210858 40.0 38.0 41.0 32.0 41.0 26-27 37.74766004813723 40.0 38.0 41.0 32.0 41.0 28-29 37.62085830633218 40.0 37.0 41.0 32.0 41.0 30-31 37.45164068180943 40.0 37.0 41.0 31.5 41.0 32-33 37.272585414777424 40.0 37.0 41.0 31.0 41.0 34-35 36.95964445816196 40.0 36.0 41.0 30.5 41.0 36-37 36.694479025185174 39.5 36.0 41.0 30.0 41.0 38-39 36.49251490867293 39.0 36.0 41.0 30.0 41.0 40-41 36.345622182565236 39.0 35.0 41.0 30.0 41.0 42-43 36.14187177993481 39.0 35.0 41.0 29.5 41.0 44-45 36.092464761816515 39.0 35.0 41.0 29.0 41.0 46-47 35.89440079984549 39.0 35.0 41.0 28.0 41.0 48-49 35.72443942419389 39.0 35.0 41.0 27.5 41.0 50-51 34.55959039847343 38.0 34.0 40.0 25.5 40.5 52-53 34.70423105492771 38.0 34.0 39.5 26.0 40.5 54-55 35.19649162951302 39.0 35.0 40.0 26.0 41.0 56-57 35.07896204771965 39.0 35.0 40.0 26.0 41.0 58-59 34.866014611454204 38.0 34.0 40.0 26.0 41.0 60-61 34.5833270467164 38.0 34.0 40.0 26.0 41.0 62-63 34.21746644433433 37.5 34.0 40.0 24.5 41.0 64-65 33.80728503604657 37.0 33.5 40.0 23.0 41.0 66-67 33.41520251097393 36.0 33.0 39.0 22.5 41.0 68-69 33.01296399537097 36.0 33.0 39.0 22.0 41.0 70-71 32.59391297923976 35.0 32.0 38.5 21.0 40.0 72-73 32.1581469703567 35.0 32.0 37.5 20.0 40.0 74-75 31.69299715740572 35.0 31.0 37.0 18.5 39.0 76-77 31.284605948068265 35.0 31.0 36.5 17.5 39.0 78-79 30.858810304959874 35.0 31.0 36.0 13.5 38.5 80-81 30.422900460368176 34.0 30.5 35.5 8.5 37.5 82-83 30.036312177641324 34.0 30.0 35.0 7.0 37.0 84-85 29.685052856101372 34.0 30.0 35.0 2.0 36.5 86-87 29.296130177733353 34.0 29.5 35.0 2.0 36.0 88-89 28.88027782360139 34.0 29.0 35.0 2.0 36.0 90-91 28.441244599900394 34.0 29.0 35.0 2.0 35.0 92-93 28.007135440649066 34.0 28.0 35.0 2.0 35.0 94-95 27.516343899753867 34.0 27.0 35.0 2.0 35.0 96-97 26.907564036627864 33.0 25.5 35.0 2.0 35.0 98-99 26.165268168296855 33.0 24.5 35.0 2.0 35.0 100-101 23.4816812156199 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2815997853344747 1101 2 0.24671587393699568 1101 3 0.2557107120973505 1101 4 0.06580889825503533 1101 5 -0.0478238005455367 1101 6 0.17688502703839504 1101 7 0.1016653635006648 1101 8 0.24471878820606463 1101 9 0.14389295217025477 1101 10-11 0.3837086103633567 1101 12-13 0.3819170420756137 1101 14-15 0.1425781492715501 1101 16-17 0.30332058907809767 1101 18-19 0.34483711215643353 1101 20-21 0.48633993514521734 1101 22-23 0.3422253531693187 1101 24-25 0.4795622454789452 1101 26-27 0.44200130859561426 1101 28-29 0.36969771515096284 1101 30-31 0.4748881548911825 1101 32-33 0.4477685893165386 1101 34-35 0.374428669827104 1101 36-37 0.327744406918832 1101 38-39 0.354503122539775 1101 40-41 0.37289451043228894 1101 42-43 0.29664390272562 1101 44-45 0.33838035138005296 1101 46-47 0.4249578298747707 1101 48-49 0.539194994459848 1101 50-51 0.4391715043454454 1101 52-53 0.5322223629663156 1101 54-55 0.5330986904187256 1101 56-57 0.6334792940989615 1101 58-59 0.4858611435723432 1101 60-61 0.46759150214125356 1101 62-63 0.48040487092319495 1101 64-65 0.4020827764675161 1101 66-67 0.4405133202001039 1101 68-69 0.4108248547461386 1101 70-71 0.3388966156288973 1101 72-73 0.30932795534670277 1101 74-75 0.33492818998587737 1101 76-77 0.17695820971767162 1101 78-79 0.19420972924321944 1101 80-81 0.01006919961440289 1101 82-83 -0.005232913788034921 1101 84-85 -0.026165288165099554 1101 86-87 -0.11578634102847118 1101 88-89 -0.19849548304485154 1101 90-91 -0.20254870346288456 1101 92-93 -0.18014731890347946 1101 94-95 -0.24175283528863645 1101 96-97 -0.34432771820860353 1101 98-99 -0.540204476228002 1101 100-101 -0.21696480171787158 1102 1 0.22304275173068433 1102 2 0.15500930791806056 1102 3 0.21846071656925758 1102 4 0.2135660213411441 1102 5 0.35848487943791696 1102 6 0.281735246846452 1102 7 0.2717546404407045 1102 8 0.2021119996075953 1102 9 0.33083613067842066 1102 10-11 0.23285543666717956 1102 12-13 0.3090306919051642 1102 14-15 0.46278626853302285 1102 16-17 0.4611955960620051 1102 18-19 0.4783941071141058 1102 20-21 0.5384959024695917 1102 22-23 0.4614332116247226 1102 24-25 0.48010356711862556 1102 26-27 0.5310017632947464 1102 28-29 0.5375392788919839 1102 30-31 0.5267595211903071 1102 32-33 0.5715857697888111 1102 34-35 0.4224492821184711 1102 36-37 0.41765343175811864 1102 38-39 0.451646203041399 1102 40-41 0.4308352489352387 1102 42-43 0.3156369771211516 1102 44-45 0.5231923071185491 1102 46-47 0.4535451333233951 1102 48-49 0.5077480442013709 1102 50-51 0.46856883034352137 1102 52-53 0.4206914998484237 1102 54-55 0.5407060675422954 1102 56-57 0.37689554211780063 1102 58-59 0.29779766942802866 1102 60-61 0.31648276721406177 1102 62-63 0.2853970213453607 1102 64-65 0.38750364037820617 1102 66-67 0.24209660732372384 1102 68-69 0.2253490139778478 1102 70-71 0.20211874311292632 1102 72-73 0.2011802282571722 1102 74-75 0.10351619789795308 1102 76-77 0.0532571884223394 1102 78-79 0.06639841768023658 1102 80-81 0.0178389456255168 1102 82-83 -0.10454691213928413 1102 84-85 -0.07847175449914445 1102 86-87 -0.19366168368147996 1102 88-89 -0.29901281096861254 1102 90-91 -0.23932975600194695 1102 92-93 -0.15495965889476793 1102 94-95 -0.1123782332055896 1102 96-97 -0.23786816379609377 1102 98-99 -0.22885377025183473 1102 100-101 0.03912472167396075 1103 1 -0.016064383217720746 1103 2 0.18455692099018606 1103 3 0.1515125690772372 1103 4 0.18568662504861777 1103 5 0.20292735548498797 1103 6 0.12719243174293382 1103 7 0.12190842148602599 1103 8 0.0965478323134974 1103 9 0.16643951829031067 1103 10-11 0.17400822859281107 1103 12-13 0.3172202092049119 1103 14-15 0.27936263496987834 1103 16-17 0.37331255823432485 1103 18-19 0.33266233078310137 1103 20-21 0.35043423257967277 1103 22-23 0.3844550952825969 1103 24-25 0.38029696216121067 1103 26-27 0.2806358874317283 1103 28-29 0.32207653327289876 1103 30-31 0.39798155149379255 1103 32-33 0.36057750744080863 1103 34-35 0.30537146644903856 1103 36-37 0.36276171821899084 1103 38-39 0.4132019259107267 1103 40-41 0.4348315877161113 1103 42-43 0.26625382365012484 1103 44-45 0.42559325982288243 1103 46-47 0.36861862836057213 1103 48-49 0.20789419770683537 1103 50-51 0.10755441675370747 1103 52-53 0.16775096021227398 1103 54-55 0.3302238593941169 1103 56-57 0.31914079458936584 1103 58-59 0.2987823602174089 1103 60-61 0.27284487359609955 1103 62-63 0.21006645361034515 1103 64-65 0.08757843947864075 1103 66-67 0.12565753660413037 1103 68-69 0.08601698962876725 1103 70-71 0.12762622056248318 1103 72-73 0.12816744843718197 1103 74-75 0.031023567085643577 1103 76-77 -0.12347487824728276 1103 78-79 -6.993295038881797E-5 1103 80-81 -0.02174404938951824 1103 82-83 -0.003199544132627352 1103 84-85 -0.15950722994092814 1103 86-87 -0.07448703270293677 1103 88-89 -0.011128788942244228 1103 90-91 -0.06189841616306069 1103 92-93 -0.14971486407559098 1103 94-95 -0.2797338389552806 1103 96-97 -0.2678881922394325 1103 98-99 -0.233648786269665 1103 100-101 0.01835763721494743 1104 1 0.10658555361253974 1104 2 0.1126175422990876 1104 3 0.18270137761746597 1104 4 0.14928840949236388 1104 5 0.10124706742013956 1104 6 0.11190183740630033 1104 7 0.06514168420992661 1104 8 0.08967463982028079 1104 9 0.1987532081336454 1104 10-11 0.08403503853440952 1104 12-13 0.18943445066752673 1104 14-15 0.0891722059296498 1104 16-17 0.06623375973092749 1104 18-19 0.20011246456227383 1104 20-21 0.10050768262147614 1104 22-23 0.12251439097379802 1104 24-25 0.24394879543638126 1104 26-27 0.23359918405356694 1104 28-29 0.20115801547580503 1104 30-31 0.31863007613539907 1104 32-33 0.3304416181001244 1104 34-35 0.13554820645753551 1104 36-37 0.12794802161230479 1104 38-39 0.2058804582084548 1104 40-41 0.20298375780639333 1104 42-43 0.07266954277067583 1104 44-45 0.13595931968128383 1104 46-47 0.1449482364376493 1104 48-49 0.12723144296505495 1104 50-51 0.01772001075217844 1104 52-53 0.09112295288620942 1104 54-55 0.0894159359877662 1104 56-57 0.19197942904082055 1104 58-59 0.06364862162380547 1104 60-61 0.11742864316587287 1104 62-63 0.13203275045672314 1104 64-65 0.04263837633595102 1104 66-67 0.13390450188646952 1104 68-69 0.14174839242988213 1104 70-71 0.036050926829936714 1104 72-73 -0.10149744081962808 1104 74-75 0.0124719532137334 1104 76-77 0.01089920024551816 1104 78-79 0.10525769752150183 1104 80-81 0.03203084990411753 1104 82-83 0.025687018161935526 1104 84-85 0.012271451421160151 1104 86-87 0.04904322249199211 1104 88-89 0.18287860746343654 1104 90-91 0.2312960248997058 1104 92-93 0.2587068663154142 1104 94-95 0.11026938040198786 1104 96-97 0.17821543860038247 1104 98-99 0.16929056807335385 1104 100-101 0.5784143411882816 1105 1 0.13406271616311116 1105 2 0.19731135986402748 1105 3 0.21005319925965793 1105 4 0.17433444130837472 1105 5 0.23923071641521432 1105 6 0.29914540817352275 1105 7 0.20089764525136644 1105 8 0.3198672071254123 1105 9 0.29133562975358274 1105 10-11 0.20226815162885714 1105 12-13 0.3552402121502851 1105 14-15 0.36476269387739535 1105 16-17 0.3932625188559271 1105 18-19 0.3807982598891826 1105 20-21 0.3667964370015824 1105 22-23 0.3939313625946852 1105 24-25 0.40759835737306105 1105 26-27 0.298045301109795 1105 28-29 0.40109056863021664 1105 30-31 0.4664388147271694 1105 32-33 0.447912870522444 1105 34-35 0.4221940459581859 1105 36-37 0.458701421248243 1105 38-39 0.5133277446932638 1105 40-41 0.49192898361947357 1105 42-43 0.3589260801283629 1105 44-45 0.25101985913007496 1105 46-47 0.44761073347208225 1105 48-49 0.42310002186788154 1105 50-51 0.25948267763718746 1105 52-53 0.29455964522991707 1105 54-55 0.2471408830275621 1105 56-57 0.15264972996397574 1105 58-59 0.1877740171195157 1105 60-61 0.3323936547866708 1105 62-63 0.31398140267454266 1105 64-65 0.37250803858734827 1105 66-67 0.17442688164212683 1105 68-69 0.26991954890446124 1105 70-71 0.1805278962253496 1105 72-73 0.3193879866726448 1105 74-75 0.3209460588738118 1105 76-77 0.28018122988644123 1105 78-79 0.1638111122739403 1105 80-81 0.03489537254841224 1105 82-83 0.0599984619758267 1105 84-85 0.15509479212377997 1105 86-87 0.18226124009011002 1105 88-89 0.22376399296546268 1105 90-91 0.1614163164800324 1105 92-93 0.25675599911168945 1105 94-95 0.26969645487800165 1105 96-97 0.31890787888499617 1105 98-99 0.36941008283397636 1105 100-101 0.4946598456067086 1106 1 0.17797190679413788 1106 2 0.23184678189149466 1106 3 0.20315737918751964 1106 4 0.20516983703874558 1106 5 0.22931462136226344 1106 6 0.20241878248445744 1106 7 0.2278108001085073 1106 8 0.2571562056811203 1106 9 0.2727429259285472 1106 10-11 0.3600966175413447 1106 12-13 0.3008023515380174 1106 14-15 0.27263713875057505 1106 16-17 0.3352574486770905 1106 18-19 0.21497488217847405 1106 20-21 0.4661365928344168 1106 22-23 0.3276490554626008 1106 24-25 0.3811740745833987 1106 26-27 0.3816717119163471 1106 28-29 0.3301978722601149 1106 30-31 0.29126634754324243 1106 32-33 0.3652716792720838 1106 34-35 0.4549191872129299 1106 36-37 0.3423358399717813 1106 38-39 0.31255636314530477 1106 40-41 0.4247556677192392 1106 42-43 0.467716909240643 1106 44-45 0.4897469239233416 1106 46-47 0.3246255130966702 1106 48-49 0.36789495775130376 1106 50-51 0.35835014108135255 1106 52-53 0.22448975720967468 1106 54-55 0.1802194785822877 1106 56-57 0.41358893369439187 1106 58-59 0.5016468833475685 1106 60-61 0.5392608839327053 1106 62-63 0.400190520312691 1106 64-65 0.3397353823264666 1106 66-67 0.37551223088151886 1106 68-69 0.45442072735853856 1106 70-71 0.6168996708370926 1106 72-73 0.43078548492565005 1106 74-75 0.5007351618806091 1106 76-77 0.5356612256592435 1106 78-79 0.5162592000816701 1106 80-81 0.48063436196976994 1106 82-83 0.5443003846132015 1106 84-85 0.5295202087197914 1106 86-87 0.6031244853486442 1106 88-89 0.6766614662321295 1106 90-91 0.5975726351156609 1106 92-93 0.4539655984536921 1106 94-95 0.617896352966504 1106 96-97 0.8146042891262795 1106 98-99 0.8135610060587375 1106 100-101 1.1004319357836359 1107 1 0.03054701131322446 1107 2 -0.04541593813645761 1107 3 -0.06906581810586232 1107 4 0.0014645756176747682 1107 5 0.010268772078426025 1107 6 -0.058659755857817686 1107 7 -0.0671301847205541 1107 8 -0.006684839566403866 1107 9 -0.03425607194993319 1107 10-11 -0.0026062635378067966 1107 12-13 -0.0029400547021509738 1107 14-15 0.00950127889552732 1107 16-17 0.10773036753597864 1107 18-19 0.14965540438165448 1107 20-21 0.10339923602496981 1107 22-23 0.06001851073392572 1107 24-25 0.13723058081087203 1107 26-27 0.10673959169735525 1107 28-29 0.1837929933275575 1107 30-31 0.21783395192281318 1107 32-33 0.08826019733736246 1107 34-35 -0.043714320204308876 1107 36-37 -3.83957468642393E-4 1107 38-39 0.1306856635124305 1107 40-41 -0.05358396897733542 1107 42-43 0.034283276133585616 1107 44-45 0.14152192863468827 1107 46-47 0.12925003296398785 1107 48-49 0.03186961920165032 1107 50-51 0.007909903192746981 1107 52-53 0.012360083170129599 1107 54-55 0.08937795128114345 1107 56-57 0.17115671035151792 1107 58-59 0.01979193538266344 1107 60-61 -0.11130474046827032 1107 62-63 -0.18112508973182173 1107 64-65 -0.047692157978794114 1107 66-67 -0.03616054368480803 1107 68-69 -0.09466199579144075 1107 70-71 -0.052877023488342445 1107 72-73 -0.1045278741873048 1107 74-75 -0.08387778384711098 1107 76-77 -0.12209354897665037 1107 78-79 -0.12220918375270884 1107 80-81 -0.04525377732754876 1107 82-83 -0.13282176340475615 1107 84-85 -0.17501408785803463 1107 86-87 0.034969707315728016 1107 88-89 0.02958051923942051 1107 90-91 0.09079375618148688 1107 92-93 -7.436043218369548E-4 1107 94-95 0.11105944157135639 1107 96-97 0.25584379374340216 1107 98-99 0.4415463414378351 1107 100-101 0.5179466784551678 1108 1 0.09967369054601605 1108 2 0.09467300722567629 1108 3 0.049884258388967595 1108 4 0.087718376622135 1108 5 0.06836624291916138 1108 6 0.07798816070048531 1108 7 0.02726662074314845 1108 8 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-2.2293609587591163 2316 90-91 -2.434405932816478 2316 92-93 -2.6645644182076573 2316 94-95 -2.809138252824404 2316 96-97 -2.90066066843762 2316 98-99 -3.405786983874158 2316 100-101 -3.970822584199542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9360.0 3 2342.0 4 2476.0 5 2802.0 6 3378.0 7 4185.0 8 5099.0 9 6307.0 10 7730.0 11 9970.0 12 12843.0 13 18888.0 14 17626.0 15 15992.0 16 19556.0 17 25119.0 18 22218.0 19 17431.0 20 18261.0 21 19714.0 22 21513.0 23 24057.0 24 27613.0 25 32017.0 26 37387.0 27 44342.0 28 52401.0 29 62704.0 30 75043.0 31 91910.0 32 114383.0 33 147071.0 34 198768.0 35 284460.0 36 435508.0 37 651874.0 38 560918.0 39 91327.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46610180398415 18.857000613057657 13.858147938063912 23.818749644894275 2 15.512139492552137 20.31856083688012 39.866472631118896 24.302827039448847 3 19.434716214531125 23.894318525745504 30.03707693165676 26.633888328066618 4 13.026234152027726 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22.760077373924613 24-25 22.695184614813222 26.160421528968676 28.611295987197295 22.533097869020807 26-27 22.502163460106924 26.178462813403957 28.76311201003231 22.556261716456806 28-29 22.370771809624816 26.65750265259658 28.59830544091397 22.37342009686463 30-31 22.603051070384836 26.263527002767333 28.376781604764805 22.756640322083022 32-33 22.479581430088793 26.335211599950675 28.462719491694454 22.722487478266075 34-35 22.83966832045598 26.47040617861553 28.25616871793099 22.433756782997495 36-37 22.852562392137955 26.18868048087956 28.533967168221736 22.424789958760748 38-39 22.57142596319321 26.510277324222137 28.409906851371105 22.508389861213548 40-41 22.73363404123828 26.573158664929906 28.232501155227492 22.460706138604326 42-43 22.460930215210176 26.524505732973445 28.062541871865363 22.952022179951015 44-45 22.65691161646213 26.47408738051748 28.11953194900148 22.749469054018913 46-47 22.873335760312656 26.77211592776793 27.79229715796314 22.562251153956268 48-49 22.765226853328453 26.728977170936652 27.802327584008353 22.70346839172654 50-51 22.445174354650728 26.961485885336025 27.958166973203767 22.63517278680948 52-53 22.79356482775817 27.019767447717925 27.445854081188024 22.740813643335876 54-55 22.681965138498445 26.854185489380058 27.50532807399176 22.958521298129746 56-57 22.630277255826183 26.990809752931956 27.510944723031354 22.867968268210507 58-59 22.881211782286666 26.734764171896373 27.5994575151911 22.78456653062586 60-61 22.720104050188176 26.82779033320909 27.933575215421317 22.518530401181415 62-63 22.488838916839033 26.77358370001829 28.017128087967873 22.720449295174806 64-65 22.407755844191012 27.00671904256674 27.768237822965343 22.8172872902769 66-67 22.51168983639268 26.94496266306566 27.43045932995067 23.112888170590992 68-69 22.443310205910798 26.75032681879264 27.792887427892797 23.013475547403765 70-71 22.771338091507086 26.568739277103575 27.54748306380706 23.112439567582275 72-73 22.6184843767688 26.60900175346974 27.723022631048522 23.049491238712935 74-75 22.390282187184873 27.19594246205898 27.361632083707583 23.052143267048564 76-77 22.544769596138206 26.78613621026547 27.36951085153965 23.299583342056668 78-79 22.595998132869088 26.8655335251035 27.39913889131076 23.13932945071665 80-81 22.707851981383893 26.760146906962596 27.2630033088373 23.26899780281621 82-83 22.737053870893313 26.67685302091823 27.608089148857072 22.97800395933139 84-85 22.60586973453155 26.749788603676123 27.45624269242417 23.188098969368156 86-87 22.387582594932823 26.629415332855494 27.69699940096934 23.286002671242343 88-89 22.60966175027492 26.573669846281035 27.35710860882243 23.459559794621615 90-91 22.616623886627437 26.485388874334998 27.411241914194743 23.48674532484282 92-93 22.661096179855242 26.577504239269572 27.34269830098578 23.418701279889408 94-95 22.717932561498273 26.43244585369709 27.37605522725301 23.473566357551622 96-97 22.527619934088758 26.19861665741961 27.681037072397196 23.592726336094437 98-99 22.551424390067254 26.413648197263086 27.282114018907894 23.752813393761766 100-101 22.825346608590174 25.76006596176692 27.67743398904195 23.73715344060096 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3278.0 1 1892.0 2 547.5 3 650.0 4 611.0 5 606.0 6 922.5 7 1672.5 8 2656.5 9 3325.0 10 3295.5 11 3149.5 12 3383.5 13 3752.5 14 4107.0 15 4327.5 16 4581.5 17 4929.5 18 5234.5 19 5512.0 20 5921.5 21 6746.0 22 8086.5 23 9933.5 24 12119.5 25 14840.0 26 19543.0 27 25166.5 28 29126.5 29 32260.5 30 35330.5 31 40504.5 32 45704.0 33 49922.5 34 55866.5 35 61573.0 36 68489.0 37 74143.0 38 77888.5 39 81490.5 40 84781.0 41 87782.5 42 89721.5 43 94160.0 44 100285.5 45 105053.5 46 109206.5 47 116499.0 48 128865.0 49 130560.0 50 121953.0 51 118506.5 52 118272.5 53 119041.5 54 117576.0 55 111214.5 56 102151.0 57 92354.5 58 85178.0 59 77124.5 60 65022.5 61 52781.5 62 41849.5 63 32273.5 64 23735.0 65 16751.5 66 11645.5 67 8510.5 68 6325.5 69 4771.0 70 3477.5 71 2093.5 72 1240.0 73 790.0 74 520.5 75 346.5 76 227.5 77 121.5 78 76.5 79 58.5 80 48.0 81 37.5 82 32.0 83 27.0 84 22.5 85 17.5 86 15.0 87 13.0 88 9.0 89 9.5 90 7.5 91 5.5 92 5.0 93 3.5 94 2.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28003356896930054 2 0.08645793846335884 3 0.08999513869737749 4 0.1050830193415987 5 0.1071489946995211 6 0.08210686914894649 7 0.09146636357347375 8 0.08802307131026973 9 0.09303149642038466 10-11 0.08686487300355566 12-13 0.09046467855145077 14-15 0.09922942249415188 16-17 0.08805437396720797 18-19 0.09877553396854771 20-21 0.10107627925350676 22-23 0.113299966787881 24-25 0.1265096880158091 26-27 0.12915476252708852 28-29 0.12131344696406487 30-31 0.11389471726970715 32-33 0.10847935761939538 34-35 0.376477054995951 36-37 0.575828025706994 38-39 0.5592532688582074 40-41 0.5218622451455057 42-43 0.5482190822874855 44-45 0.5700526855018927 46-47 0.5580794192230242 48-49 0.5801634436929372 50-51 0.5720560555459387 52-53 0.5754993478091427 54-55 0.564919049764025 56-57 0.537544876271553 58-59 0.5326616617891909 60-61 0.4814974690236733 62-63 0.5549961075146098 64-65 0.5882551805114666 66-67 0.6012927371262345 68-69 0.6044543054769946 70-71 0.6000406308487057 72-73 0.592042802000991 74-75 0.5884742991100341 76-77 0.5719151935897167 78-79 0.5493616292657304 80-81 0.5436645457029747 82-83 0.557750741325173 84-85 0.5671728410635767 86-87 0.557750741325173 88-89 0.5708352519253482 90-91 0.5847649342628553 92-93 0.5932479542931125 94-95 0.5928253684244464 96-97 0.589460332803588 98-99 0.593482724220149 100-101 0.5706943899691261 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3194617.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.16451060467201 #Duplication Level Percentage of deduplicated Percentage of total 1 70.84674471577411 32.70605297737845 2 14.201824672989035 13.1124057144379 3 5.5303888330399555 7.659230817924982 4 2.814116720090499 5.196492846696107 5 1.668685062622564 3.851701463464857 6 1.0443695094977976 2.892768437784431 7 0.7167126615228533 2.3160682484362125 8 0.5255362664014281 1.9408899634742773 9 0.3916704446070385 1.6273146954238407 >10 2.065237182325846 16.87512890275712 >50 0.1271418208577227 4.057301532362299 >100 0.06322887645933276 5.102493649642815 >500 0.003053836274313452 0.9418640796873008 >1k 0.0010858084526383768 0.9946841578064516 >5k 2.0358908486969567E-4 0.7256025127229992 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8762 0.27427388009266834 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7345 0.2299180152112131 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7054 0.2208089420421916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4465 0.13976636322914454 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3808 0.11920051762073514 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.130265693821826E-5 7 3.130265693821826E-5 0.0 0.0 0.0 3.130265693821826E-5 8 3.130265693821826E-5 0.0 0.0 0.0 6.260531387643652E-5 9 3.130265693821826E-5 0.0 0.0 0.0 9.390797081465477E-5 10-11 3.130265693821826E-5 0.0 0.0 4.695398540732739E-5 1.2521062775287304E-4 12-13 3.130265693821826E-5 0.0 0.0 6.260531387643652E-5 2.1911859856752782E-4 14-15 3.130265693821826E-5 1.565132846910913E-5 0.0 1.0955929928376391E-4 2.1911859856752782E-4 16-17 3.130265693821826E-5 3.130265693821826E-5 0.0 1.5651328469109128E-4 2.3476992703663695E-4 18-19 9.390797081465477E-5 3.130265693821826E-5 0.0 1.8781594162930955E-4 2.504212555057461E-4 20-21 1.5651328469109128E-4 3.130265693821826E-5 0.0 2.660725839748552E-4 3.2867789785129167E-4 22-23 1.8781594162930955E-4 3.130265693821826E-5 0.0 4.5388852560416474E-4 3.5998055478951E-4 24-25 2.8172391244396435E-4 3.130265693821826E-5 0.0 6.886584526408017E-4 4.0693454019683737E-4 26-27 2.8172391244396435E-4 3.130265693821826E-5 0.0 0.0011581983067140756 4.5388852560416474E-4 28-29 3.4432922632040083E-4 3.130265693821826E-5 0.0 0.003928483445746391 4.695398540732739E-4 30-31 3.756318832586191E-4 3.130265693821826E-5 0.0 0.011848055651115613 4.695398540732739E-4 32-33 3.912832117277282E-4 3.130265693821826E-5 0.0 0.027436778806348303 5.321451679497104E-4 34-35 4.225858686659465E-4 3.130265693821826E-5 0.0 0.05144591667796171 5.634478248879287E-4 36-37 4.3823719713505563E-4 6.260531387643652E-5 0.0 0.08816393326649172 5.634478248879287E-4 38-39 4.695398540732739E-4 9.390797081465477E-5 0.0 0.14203580585716535 5.790991533570378E-4 40-41 4.695398540732739E-4 9.390797081465477E-5 0.0 0.20271600633190145 5.947504818261469E-4 42-43 6.10401810295256E-4 9.390797081465477E-5 0.0 0.26666733445668134 6.10401810295256E-4 44-45 8.138690803936747E-4 9.390797081465477E-5 0.0 0.3483829203939001 6.730071241716926E-4 46-47 8.45171737331893E-4 9.390797081465477E-5 0.0 0.4480975340705944 7.512637665172382E-4 48-49 8.764743942701113E-4 9.390797081465477E-5 0.0 0.5540413764779941 7.825664234554565E-4 50-51 8.764743942701113E-4 9.390797081465477E-5 0.0 0.6679204424192322 8.295204088627838E-4 52-53 8.764743942701113E-4 9.390797081465477E-5 0.0 0.7827698907255549 8.451717373318929E-4 54-55 8.764743942701113E-4 9.390797081465477E-5 0.0 0.9145697277639229 9.077770512083295E-4 56-57 9.077770512083295E-4 9.390797081465477E-5 0.0 1.0765922800761407 9.860336935538752E-4 58-59 9.077770512083295E-4 9.390797081465477E-5 0.0 1.2510889411782382 0.0010329876789612025 60-61 9.077770512083295E-4 1.0955929928376391E-4 0.0 1.4398596138441635 0.0010329876789612025 62-63 9.077770512083295E-4 1.2521062775287304E-4 0.0 1.6340926001458078 0.001095592992837639 64-65 9.077770512083295E-4 1.2521062775287304E-4 0.0 1.8489227347127999 0.001111244321306748 66-67 9.390797081465478E-4 1.2521062775287304E-4 0.0 2.1013630115910606 0.0011425469782449665 68-69 9.70382365084766E-4 1.2521062775287304E-4 0.0 2.3897700412913347 0.0011581983067140756 70-71 0.0010016850220229843 1.4086195622198218E-4 0.0 2.6951744137090614 0.0011895009636522938 72-73 0.0010173363504920934 1.5651328469109128E-4 0.0 3.006761060872086 0.0014086195622198218 74-75 0.0010486390074303117 1.5651328469109128E-4 0.0 3.3413238582277627 0.0014712248760962582 76-77 0.0010642903358994208 1.5651328469109128E-4 0.0 3.709583965777431 0.0014712248760962582 78-79 0.0010642903358994208 1.5651328469109128E-4 0.0 4.128804798822519 0.0015338301899726946 80-81 0.001220803620590512 1.5651328469109128E-4 0.0 4.577105800163212 0.0015338301899726946 82-83 0.0012521062775287303 1.5651328469109128E-4 0.0 5.0370513898849225 0.0015338301899726946 84-85 0.0012521062775287303 1.5651328469109128E-4 0.0 5.510097141535276 0.0015338301899726946 86-87 0.0012521062775287303 1.5651328469109128E-4 0.0 6.017278440576757 0.001596435503849131 88-89 0.0012521062775287303 1.5651328469109128E-4 0.0 6.5735110030404265 0.0016590408177255677 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9180 0.0 22.531029 2 GTACATG 9630 0.0 22.056833 1 GAGTACT 8590 0.0 20.9651 12-13 ACATGGG 9370 0.0 20.811659 3 GGTATCA 14460 0.0 20.755196 1 GTATCAA 18370 0.0 20.44125 1 CATGGGG 6750 0.0 19.493816 4 GTACTTT 9330 0.0 18.99924 14-15 AGAGTAC 13000 0.0 18.603703 10-11 AGTACAT 7220 0.0 18.486477 2 TATCAAC 20535 0.0 18.02413 2 CAACGCA 20635 0.0 17.89147 5 ATCAACG 20765 0.0 17.801973 3 TCAACGC 20825 0.0 17.75096 4 AGTACTT 9140 0.0 17.735762 12-13 AACGCAG 21065 0.0 17.591454 6 GAGTACG 355 1.6261765E-9 17.362265 1 GAGTACA 8145 0.0 17.34669 1 GTCTACG 195 2.3092508E-4 17.019815 1 CCTATAC 605 0.0 16.42907 3 >>END_MODULE