FastQCFastQC Report
Fri 27 May 2016
SRR939046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194617
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88210.27612073685202326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78420.24547543570950758No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74760.23401866327011972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48550.15197439943504965No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34700.10862021957561735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG95000.021.3289322
GTACATG100800.021.2713241
GAGTACT96450.020.937112-13
GGTATCA155850.020.7589591
GTATCAA197000.020.558681
ACATGGG95850.020.0011083
TAGGACG1708.0201185E-519.5395224
AGAGTAC143500.019.51278710-11
GTACTTT102200.019.4108514-15
AGTACTT99250.019.07926212-13
CAACGCA222500.017.9788685
ATCAACG223850.017.9340383
TATCAAC224850.017.8964862
TCAACGC225150.017.767264
CATGGGG68500.017.6650314
AACGCAG228550.017.5444726
AGTACAT75550.016.3934672
GAGTACA85550.016.1517851
ACGCAGA247900.015.9070387
CGCAGAG247050.015.82731158
Produced by FastQC (version 0.11.5)