##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939046_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3194617 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10765108931681 33.0 31.0 34.0 30.0 34.0 2 32.34819260023971 34.0 31.0 34.0 30.0 34.0 3 32.446170855536046 34.0 31.0 34.0 30.0 34.0 4 36.033419029573814 37.0 35.0 37.0 35.0 37.0 5 35.944780235001566 37.0 35.0 37.0 35.0 37.0 6 35.979030663143654 37.0 35.0 37.0 35.0 37.0 7 35.91657591504709 37.0 35.0 37.0 35.0 37.0 8 35.88249358217276 37.0 35.0 37.0 35.0 37.0 9 37.55922760067952 39.0 37.0 39.0 35.0 39.0 10-11 37.476976739308654 39.0 37.0 39.0 34.5 39.0 12-13 37.3994624394724 39.0 37.0 39.0 34.0 39.0 14-15 38.65547231483461 40.0 38.0 41.0 34.0 41.0 16-17 38.6380686636301 40.0 38.0 41.0 34.0 41.0 18-19 38.72739658619484 40.0 38.0 41.0 34.0 41.0 20-21 38.71729631439387 40.0 38.0 41.0 34.0 41.0 22-23 38.6551830782845 40.0 38.0 41.0 34.0 41.0 24-25 38.59105582922773 40.0 38.0 41.0 34.0 41.0 26-27 38.43038586472181 40.0 38.0 41.0 34.0 41.0 28-29 38.24821582681116 40.0 38.0 41.0 33.5 41.0 30-31 38.086467329260444 40.0 37.5 41.0 33.0 41.0 32-33 37.9401812486442 40.0 37.0 41.0 33.0 41.0 34-35 37.91962401126645 40.0 37.0 41.0 33.0 41.0 36-37 37.820455628953326 40.0 37.0 41.0 33.0 41.0 38-39 37.691643160979865 40.0 37.0 41.0 33.0 41.0 40-41 37.53645257005769 40.0 37.0 41.0 32.0 41.0 42-43 37.3635814559304 40.0 37.0 41.0 32.0 41.0 44-45 37.12607849391648 40.0 36.0 41.0 31.0 41.0 46-47 36.83855059933632 40.0 36.0 41.0 31.0 41.0 48-49 36.55341141050711 39.0 36.0 41.0 30.0 41.0 50-51 36.283958296096216 39.0 35.0 41.0 30.0 41.0 52-53 36.04780463510962 39.0 35.0 40.0 29.0 41.0 54-55 35.78959590461079 38.5 35.0 40.0 28.5 41.0 56-57 35.482656606410096 38.0 34.5 40.0 28.0 41.0 58-59 35.16074321272315 38.0 34.0 40.0 27.5 41.0 60-61 34.72933296855304 37.5 34.0 40.0 26.5 41.0 62-63 34.39848016209768 37.0 34.0 40.0 26.0 41.0 64-65 34.12617428004672 36.5 33.0 39.0 26.0 41.0 66-67 33.65332808283434 36.0 33.0 39.0 25.5 40.0 68-69 33.217756306937574 35.5 32.5 39.0 25.0 40.0 70-71 32.75479627135272 35.0 32.0 38.0 24.0 40.0 72-73 32.23064376731233 35.0 31.5 37.0 22.0 39.5 74-75 31.759879979352768 35.0 31.0 37.0 21.5 39.0 76-77 29.794295529010206 32.5 28.5 35.0 18.5 37.0 78-79 30.7957565805228 34.0 30.5 36.0 20.0 37.5 80-81 30.80646881926691 34.0 31.0 35.0 19.5 37.0 82-83 30.4811219936537 34.0 30.5 35.0 18.0 37.0 84-85 30.06855657501353 34.0 30.0 35.0 15.5 36.0 86-87 29.48617064267798 34.0 29.5 35.0 7.5 36.0 88-89 28.895870303075455 34.0 29.0 35.0 2.0 35.5 90-91 28.412649309760766 33.0 28.5 35.0 2.0 35.0 92-93 28.02614570072093 33.0 27.0 35.0 2.0 35.0 94-95 27.5899999906092 33.0 27.0 35.0 2.0 35.0 96-97 26.91913850705734 33.0 25.5 34.5 2.0 35.0 98-99 25.941886773907484 32.0 24.5 34.0 2.0 35.0 100-101 21.510024988911034 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19106696884327334 1101 2 0.09236808919465744 1101 3 0.14990912531450817 1101 4 0.07110355171915472 1101 5 0.10533751500399546 1101 6 0.07748038238308652 1101 7 0.12583028134398688 1101 8 0.12037557224871165 1101 9 0.12576766036253417 1101 10-11 0.07910504073871039 1101 12-13 0.1703516673880756 1101 14-15 0.24684631085312247 1101 16-17 0.1663496194403038 1101 18-19 0.24189128160200113 1101 20-21 0.22871466351589476 1101 22-23 0.1889398343776989 1101 24-25 0.23199479927154698 1101 26-27 0.2602152159970714 1101 28-29 0.27189048851300157 1101 30-31 0.2571660982855093 1101 32-33 0.2651214274452016 1101 34-35 0.4343321916424685 1101 36-37 0.37344758882542095 1101 38-39 0.3773682771462532 1101 40-41 0.44243441280883644 1101 42-43 0.44519429919142084 1101 44-45 0.4578654816044505 1101 46-47 0.39984027370645236 1101 48-49 0.11653615063134737 1101 50-51 0.25062186534481157 1101 52-53 0.38247517043914314 1101 54-55 0.4517453756612255 1101 56-57 0.3167909809330496 1101 58-59 0.2680882490577332 1101 60-61 0.4248735393840235 1101 62-63 0.41990309259037417 1101 64-65 0.34227665966788123 1101 66-67 0.4178119419095623 1101 68-69 0.3713035355760539 1101 70-71 0.3123353555819506 1101 72-73 0.17866082753876356 1101 74-75 0.22156503958821716 1101 76-77 -0.0030123609304339993 1101 78-79 0.1757687800187817 1101 80-81 0.29674119243462016 1101 82-83 0.3623251843234385 1101 84-85 0.3490938140110025 1101 86-87 0.44866347178031774 1101 88-89 0.45767004274276957 1101 90-91 0.3095749612515455 1101 92-93 0.36351447545476745 1101 94-95 0.30978261530523454 1101 96-97 0.22364218868407804 1101 98-99 0.1480338149449949 1101 100-101 0.31650323743727427 1102 1 0.06266812593000992 1102 2 0.09661364086131385 1102 3 0.0957017670502367 1102 4 0.06315768984566716 1102 5 0.08070083816346596 1102 6 0.08833265286436642 1102 7 0.12372782232488078 1102 8 0.16836223116848714 1102 9 0.1361056062752013 1102 10-11 0.24163011378538357 1102 12-13 0.23604177987918007 1102 14-15 0.27947621866115213 1102 16-17 0.23585653256758832 1102 18-19 0.14496776623663976 1102 20-21 0.17060156334449772 1102 22-23 0.2526849983686219 1102 24-25 0.25045323228425076 1102 26-27 0.25670167693832724 1102 28-29 0.26147513995486094 1102 30-31 0.16499517160249155 1102 32-33 0.2727077148322792 1102 34-35 0.22843204525099026 1102 36-37 0.186896392330695 1102 38-39 0.3464259899278872 1102 40-41 0.27215930326919135 1102 42-43 0.214983925205253 1102 44-45 0.33407204088792497 1102 46-47 0.41963657997713 1102 48-49 0.33867561496087717 1102 50-51 0.2387309529453958 1102 52-53 0.2296949358726863 1102 54-55 0.30135082092076715 1102 56-57 0.2878417290224391 1102 58-59 0.21177694113852397 1102 60-61 0.0381759403677151 1102 62-63 0.17744565465990547 1102 64-65 0.17421470265959016 1102 66-67 0.2114619547511154 1102 68-69 0.19740905183402901 1102 70-71 0.31508298386993516 1102 72-73 0.3940458896894512 1102 74-75 0.3813405520412054 1102 76-77 0.06881563780374123 1102 78-79 0.1318114201534577 1102 80-81 0.20078736327826974 1102 82-83 0.18109610981395363 1102 84-85 0.14337734707849492 1102 86-87 0.20328896995633272 1102 88-89 0.4013971601026256 1102 90-91 0.415421919548411 1102 92-93 0.3642564256348493 1102 94-95 0.433576552900373 1102 96-97 0.26837721878056797 1102 98-99 0.37939783064282295 1102 100-101 0.9606319320885852 1103 1 0.06904179664665122 1103 2 0.026685339735877278 1103 3 0.05620095262581515 1103 4 0.05015937774642509 1103 5 0.08089964629885316 1103 6 0.07519064775637929 1103 7 0.13396742549596752 1103 8 0.07782776206986597 1103 9 0.08757772146581289 1103 10-11 0.12143041987117442 1103 12-13 0.051671347176231563 1103 14-15 0.13124014876336076 1103 16-17 0.06317838030221168 1103 18-19 0.18671255367433304 1103 20-21 0.1759398570392321 1103 22-23 0.16778555886977387 1103 24-25 0.1778050980789132 1103 26-27 0.1177746503502064 1103 28-29 0.23939267394663233 1103 30-31 0.24903245304886923 1103 32-33 0.18204579200330073 1103 34-35 0.12013820289008947 1103 36-37 -0.06332234582816199 1103 38-39 0.19893003744500248 1103 40-41 0.26064909958926563 1103 42-43 0.05165762889254921 1103 44-45 0.14022672739603337 1103 46-47 0.24650569631433683 1103 48-49 0.09082584304891839 1103 50-51 -0.09455218625474515 1103 52-53 0.04820968162955808 1103 54-55 0.13949983844442926 1103 56-57 0.0055966733906060995 1103 58-59 0.006912047013699407 1103 60-61 -0.04144917835125028 1103 62-63 0.011474816467085702 1103 64-65 0.05008707177346139 1103 66-67 0.06945143366711903 1103 68-69 0.015760420608359027 1103 70-71 0.03256874989637737 1103 72-73 0.048341670921765 1103 74-75 0.12044916328764188 1103 76-77 -0.02976317928785477 1103 78-79 -0.04370024014052376 1103 80-81 0.0316213240382659 1103 82-83 0.16978176167335235 1103 84-85 0.16361198004619837 1103 86-87 -0.03990012905897444 1103 88-89 0.2967999680214888 1103 90-91 0.42526587484610445 1103 92-93 0.3202327261303921 1103 94-95 0.14140142458546734 1103 96-97 0.12492484661567715 1103 98-99 0.2862581876116863 1103 100-101 0.5644629254895328 1104 1 0.02067190061295321 1104 2 0.036738391497195266 1104 3 0.023072413699999572 1104 4 0.0771159488874531 1104 5 0.07382788168526844 1104 6 0.014450844867610613 1104 7 0.0028302889925129193 1104 8 0.047694578071421745 1104 9 0.1385339246803028 1104 10-11 0.05116186054708294 1104 12-13 0.05784818457704688 1104 14-15 0.045432644685057255 1104 16-17 0.06221122045165117 1104 18-19 0.07084166342735898 1104 20-21 0.11966738579025815 1104 22-23 0.12303297594427676 1104 24-25 0.02712700404662627 1104 26-27 -0.017021732066154982 1104 28-29 0.027136120931388064 1104 30-31 -0.02706422506139461 1104 32-33 0.04827527536409093 1104 34-35 0.071067352682185 1104 36-37 0.02847884398135392 1104 38-39 0.1490979039309579 1104 40-41 0.21760980990392653 1104 42-43 0.10285271382677053 1104 44-45 0.12229382529425692 1104 46-47 0.1223131733392151 1104 48-49 0.07774659545870577 1104 50-51 0.09809577567568084 1104 52-53 0.0015219674002793226 1104 54-55 0.02338059777988377 1104 56-57 -0.018792257431087478 1104 58-59 -0.06311221990618776 1104 60-61 0.059169803953103894 1104 62-63 0.11848002966158333 1104 64-65 0.022299419496512485 1104 66-67 0.07164276088096955 1104 68-69 0.08621564854697539 1104 70-71 0.007270283013831147 1104 72-73 0.0941463614441389 1104 74-75 0.09958962195137033 1104 76-77 -0.06477355427534093 1104 78-79 0.1049556105678704 1104 80-81 0.1964759524730333 1104 82-83 0.2184825210629171 1104 84-85 0.04500436558993215 1104 86-87 0.07861766803494774 1104 88-89 0.15542175847108908 1104 90-91 0.23830129589204674 1104 92-93 0.25665096121922204 1104 94-95 0.2254390937708095 1104 96-97 0.10782410778670837 1104 98-99 0.08184685655625401 1104 100-101 0.8247136025014292 1105 1 0.01717180298561516 1105 2 0.002010771777726461 1105 3 0.08210105315278327 1105 4 0.0671660432605492 1105 5 0.0329217110074822 1105 6 0.11705308588746988 1105 7 -0.016116133542816158 1105 8 0.1537781260799278 1105 9 -0.008639711318785714 1105 10-11 0.05611702545330388 1105 12-13 0.09143760597233808 1105 14-15 0.13947695700017704 1105 16-17 0.10099572856731953 1105 18-19 0.04342801113605077 1105 20-21 0.05845152649120422 1105 22-23 -0.018025251941033105 1105 24-25 0.06466135732323863 1105 26-27 0.07904855751963424 1105 28-29 0.14073650178299602 1105 30-31 0.1044090449926216 1105 32-33 0.10787193797870032 1105 34-35 0.18982996288035991 1105 36-37 0.16100822141284965 1105 38-39 0.22467070348417195 1105 40-41 0.17990177443896016 1105 42-43 0.1896846751285537 1105 44-45 0.19516119277826505 1105 46-47 0.2557176801531895 1105 48-49 0.14716320386389725 1105 50-51 0.251183581495944 1105 52-53 0.3700558881772622 1105 54-55 0.26129386061158044 1105 56-57 0.27962213808770997 1105 58-59 0.2502095556097146 1105 60-61 0.20029752570888348 1105 62-63 0.14921484861010725 1105 64-65 0.2136087491011054 1105 66-67 0.32775019084647283 1105 68-69 0.24045739903668562 1105 70-71 0.13627079949394982 1105 72-73 0.1085437173178434 1105 74-75 0.2755752381898411 1105 76-77 0.19868899356730552 1105 78-79 0.4296820440626554 1105 80-81 0.4206978205774483 1105 82-83 0.4200932672087383 1105 84-85 0.33006302597485515 1105 86-87 0.26462950718707745 1105 88-89 0.4885654008316962 1105 90-91 0.570720711345718 1105 92-93 0.6458301252352534 1105 94-95 0.5397380874743725 1105 96-97 0.36646937621990716 1105 98-99 0.5186299516998716 1105 100-101 1.037462630951925 1106 1 -0.09884632480845568 1106 2 -0.1368207031454105 1106 3 -0.12039643387684862 1106 4 -0.09246328234586088 1106 5 0.002524600247546971 1106 6 0.027117624373396154 1106 7 -0.011626375074698103 1106 8 -0.042423622636142966 1106 9 -0.09776946578908507 1106 10-11 0.04133883451343934 1106 12-13 -0.020127730847612213 1106 14-15 0.09651704246164883 1106 16-17 0.03403151965530782 1106 18-19 -0.07608390325173531 1106 20-21 -0.05806294879157292 1106 22-23 0.07235801802530517 1106 24-25 0.15906097654779927 1106 26-27 0.05300593082455407 1106 28-29 0.02944089454530996 1106 30-31 0.09449704606089426 1106 32-33 0.04239052494795459 1106 34-35 0.02915428310856072 1106 36-37 0.0051408759369735435 1106 38-39 0.13991089593633177 1106 40-41 0.20321269789310037 1106 42-43 0.19428298080897122 1106 44-45 0.10290036324818175 1106 46-47 0.1217790220261108 1106 48-49 0.0713849613129014 1106 50-51 0.11270549943098018 1106 52-53 0.17773593113737718 1106 54-55 0.14356734306011987 1106 56-57 0.1382573145936732 1106 58-59 0.18045885783546822 1106 60-61 0.2605237176187174 1106 62-63 0.1926575934373176 1106 64-65 0.17441245314160625 1106 66-67 0.3421311604311299 1106 68-69 0.21556977612232942 1106 70-71 0.13002861796532983 1106 72-73 0.26836525341245476 1106 74-75 0.34580129083154887 1106 76-77 0.11461006376908855 1106 78-79 0.3420415368645422 1106 80-81 0.3890941973124633 1106 82-83 0.6115418768623115 1106 84-85 0.4728506371743073 1106 86-87 0.5244387958792451 1106 88-89 0.680621260455986 1106 90-91 0.9089138876993026 1106 92-93 0.8005890603092745 1106 94-95 0.9260614049720992 1106 96-97 0.7882154380041619 1106 98-99 0.8950097301324789 1106 100-101 1.1777530418502273 1107 1 -0.1261980144629824 1107 2 -0.08641455680007937 1107 3 -0.09466742888889002 1107 4 -0.1489021759842899 1107 5 -0.12985492476788352 1107 6 -0.06884541926135057 1107 7 -0.08547601802341376 1107 8 -0.05801494308289534 1107 9 -0.06563040140985521 1107 10-11 -0.04146428702635774 1107 12-13 -0.025793983940317844 1107 14-15 -0.012946636497119357 1107 16-17 -0.13371970023959534 1107 18-19 -0.06352371732139517 1107 20-21 -0.0710848668634867 1107 22-23 -0.005922615632549366 1107 24-25 -0.07683703433399103 1107 26-27 -0.033235967884330364 1107 28-29 -0.06339527112260868 1107 30-31 -0.10204080202265686 1107 32-33 -0.10484672550234819 1107 34-35 -0.21411203048349137 1107 36-37 -0.06954346387200161 1107 38-39 -0.11666284734747023 1107 40-41 -0.05129341148939659 1107 42-43 -0.2761954816664698 1107 44-45 -0.27118850689418394 1107 46-47 -0.4115912606627461 1107 48-49 -0.31075414350707575 1107 50-51 -0.33714444520565223 1107 52-53 -0.2544841764835155 1107 54-55 -0.3268561200098006 1107 56-57 -0.17557566272635228 1107 58-59 -0.18128072483228408 1107 60-61 -0.2425398705910311 1107 62-63 -0.2766214978107371 1107 64-65 -0.3421333698557518 1107 66-67 -0.12728755246954648 1107 68-69 -0.2165729885845522 1107 70-71 -0.334434385633088 1107 72-73 -0.16867832229301882 1107 74-75 -0.23263716281163482 1107 76-77 -0.5258610465799372 1107 78-79 -0.311878040254733 1107 80-81 -0.16918131250371715 1107 82-83 -0.1311132760562579 1107 84-85 -0.36068789921062816 1107 86-87 -0.29476552044174653 1107 88-89 -0.12043395975597448 1107 90-91 0.10755192447934547 1107 92-93 0.11548502246321135 1107 94-95 0.15658271845495975 1107 96-97 0.1324182984585356 1107 98-99 0.11780039188047553 1107 100-101 0.6887697058612865 1108 1 -0.052030231128703974 1108 2 -0.08740696676281345 1108 3 -0.04229778177236909 1108 4 -0.09880632008023582 1108 5 -0.08710244849035575 1108 6 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-1.288462908503682 2316 56-57 -1.3507331295374954 2316 58-59 -1.1965090974639665 2316 60-61 -1.0025026289784407 2316 62-63 -1.1507620564281495 2316 64-65 -1.2289108434251474 2316 66-67 -1.2746411203671855 2316 68-69 -1.3020105538073707 2316 70-71 -1.2596809909963795 2316 72-73 -1.1870049798379512 2316 74-75 -1.4764189014743465 2316 76-77 -1.4878063412675893 2316 78-79 -1.5720941910758093 2316 80-81 -1.5833847781970682 2316 82-83 -1.8394762516299217 2316 84-85 -1.9715319853760924 2316 86-87 -2.14532198395273 2316 88-89 -2.4368835305521515 2316 90-91 -2.6150386485934227 2316 92-93 -2.8844807032550897 2316 94-95 -2.930770435948265 2316 96-97 -3.1127262771555273 2316 98-99 -3.480427411687984 2316 100-101 -3.9333415526089937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 14.0 5 50.0 6 148.0 7 384.0 8 918.0 9 1770.0 10 2865.0 11 4476.0 12 6405.0 13 8716.0 14 10573.0 15 13756.0 16 17582.0 17 24329.0 18 23866.0 19 18033.0 20 17395.0 21 18743.0 22 20726.0 23 22744.0 24 26326.0 25 31727.0 26 38155.0 27 45964.0 28 56562.0 29 68692.0 30 83028.0 31 100926.0 32 125134.0 33 159443.0 34 214703.0 35 307428.0 36 478392.0 37 717760.0 38 493700.0 39 33169.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.1069933291807 19.753463162784207 14.491039681098005 23.648503826937088 2 15.8963656676215 20.999450012317595 39.02843439448297 24.075749925577934 3 19.398726044467928 24.211540851375922 30.258087276189915 26.13164582796623 4 13.420857648976387 16.909789185996317 36.645394424433356 33.02395874059395 5 13.785956703267946 37.2577713808839 34.529391104881164 14.426880810966985 6 32.31833424789263 36.13650713058873 16.98779540708636 14.557363214432279 7 28.161278801183364 32.68779950773442 21.01037463958903 18.140547051493183 8 25.748563912356314 35.555999357669485 19.7112517713391 18.984184958635105 9 25.828667411461215 16.724383548951252 20.645416962346346 36.801532077241184 10-11 25.164503287874574 26.26486993589529 27.34809837924233 21.22252839698781 12-13 25.73832794353752 23.664073032854958 27.86974150578927 22.727857517818258 14-15 22.706273364787673 25.177194793364695 26.102262349246498 26.014269492601134 16-17 22.2269629381058 28.16284181674528 26.8619848566996 22.74821038844932 18-19 22.099174955871078 27.189816494434233 28.66983741713013 22.04117113256456 20-21 22.99635605770582 26.281742067984986 28.530822317667504 22.19107955664169 22-23 22.61674873701605 26.1478762555887 28.543546847712886 22.691828159682366 24-25 22.626108231440575 26.2461196443893 28.7134263669166 22.41434575725353 26-27 22.441516463475903 26.267468056421162 28.849671181240193 22.44134429886274 28-29 22.339735874441285 26.753864391255668 28.689276367088762 22.217123367214285 30-31 22.506172101381793 26.37500833433241 28.43328323864801 22.685536325637784 32-33 22.42595904297761 26.36690094618541 28.566851675803388 22.64028833503359 34-35 22.784163058039436 26.520487199501275 28.391317883134164 22.30403185932513 36-37 22.80055997926374 26.25123497215756 28.659571550749373 22.28863349782933 38-39 22.532919941650974 26.618400417127592 28.473544377645105 22.375135263576333 40-41 22.72059088226962 26.66810140411232 28.27031705610658 22.340990657511476 42-43 22.443046424471312 26.594367522043072 28.095229524220187 22.86735652926542 44-45 22.639434296258848 26.568026407020582 28.1390500547016 22.653489242018967 46-47 22.86086430635621 26.871137241251507 27.773532777730477 22.494465674661804 48-49 22.731521363497492 26.824308334543705 27.791344039706907 22.652826262251892 50-51 22.480607900832656 27.066002003380706 27.88629249358292 22.56709760220372 52-53 22.835507355028785 27.08291792099022 27.395521904503735 22.686052819477265 54-55 22.679401004877896 26.928126282430725 27.45642122357704 22.936051489114345 56-57 22.582002787814627 27.0971136759117 27.44667044594078 22.874213090332894 58-59 22.85713212477646 26.855680929966702 27.51150453780103 22.77568240745581 60-61 22.673202410804326 26.923554300773933 27.85738333476025 22.545859953661495 62-63 22.47491639842898 26.871030236175415 27.897506962493466 22.75654640290213 64-65 22.34072906830768 27.080695101736158 27.68068981157803 22.897886018378134 66-67 22.479913165084163 27.022687734724656 27.380263525500716 23.11713557469047 68-69 22.378425096261196 26.84258741452471 27.725368426703678 23.053619062510418 70-71 22.71424797221325 26.664034112394102 27.43500314278774 23.18671477260491 72-73 22.56420722734525 26.71936886330975 27.57529306329992 23.141130846045083 74-75 22.309967047693043 27.304712896725963 27.188971322696897 23.196348732884097 76-77 22.38274361037259 26.88374480792333 27.348160062481995 23.385351519222084 78-79 22.42885643948589 26.98345919358971 27.319008287228396 23.268676079696 80-81 22.522233889419905 26.7773142723078 27.275386576135197 23.4250652621371 82-83 22.574024143430048 26.840436089903125 27.465753714099954 23.119786052566873 84-85 22.35972262089634 26.87680557638052 27.348755735038033 23.41471606768511 86-87 22.111732101015726 26.752280618819217 27.5686497573573 23.567337522807755 88-89 22.29585744494755 26.67516900399724 27.271328206988787 23.75764534406642 90-91 22.242118932072756 26.631801098229285 27.293511960874934 23.83256800882302 92-93 22.23531021089539 26.73544277764752 27.231793357388383 23.797453654068704 94-95 22.240913386487332 26.57457842364202 27.2844757290154 23.900032460855243 96-97 21.97268330368437 26.372776210377314 27.6250601404932 24.029480345445116 98-99 21.907136584313015 26.642744129068387 27.18510969939584 24.265009587222757 100-101 22.04861722492756 25.951505481814124 27.836822571529503 24.163054721728813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 691.0 1 498.0 2 373.0 3 466.0 4 424.5 5 456.0 6 766.0 7 1396.5 8 2206.0 9 2761.5 10 2702.0 11 2513.0 12 2749.0 13 2979.5 14 3182.0 15 3423.0 16 3582.5 17 3859.5 18 4258.5 19 4679.0 20 5334.5 21 6446.0 22 7954.0 23 9892.5 24 12113.0 25 15204.5 26 20113.5 27 25779.5 28 29629.5 29 32560.5 30 35856.0 31 41340.0 32 46451.0 33 50425.0 34 56083.0 35 61876.0 36 68796.5 37 74690.0 38 78913.5 39 82159.5 40 85199.5 41 88664.0 42 90993.0 43 95041.5 44 101487.5 45 106179.0 46 109306.0 47 117647.5 48 130904.5 49 131928.5 50 122387.0 51 118954.5 52 119274.5 53 119773.5 54 117894.5 55 111606.5 56 102245.5 57 91938.5 58 85082.5 59 77098.0 60 64782.0 61 52049.5 62 40962.5 63 31449.0 64 23067.5 65 16245.5 66 11065.0 67 7941.5 68 5834.5 69 4449.5 70 3302.0 71 1898.5 72 1058.0 73 689.0 74 443.5 75 259.5 76 129.0 77 53.0 78 34.0 79 17.5 80 9.0 81 9.5 82 8.5 83 5.5 84 2.0 85 1.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39751244045843365 2 0.0 3 0.0 4 0.0 5 3.4432922632040083E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012364549490596212 16-17 0.0021598833287370597 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002300745284959042 36-37 0.006667465927840489 38-39 0.01222368753437423 40-41 0.002253791299551715 42-43 6.10401810295256E-4 44-45 3.756318832586191E-4 46-47 2.8172391244396435E-4 48-49 2.8172391244396435E-4 50-51 5.321451679497104E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.504212555057461E-4 60-61 0.002551166540464788 62-63 0.0 64-65 1.7216461316020042E-4 66-67 0.001549481518441804 68-69 0.001674692146194677 70-71 3.130265693821826E-5 72-73 0.0 74-75 0.0 76-77 0.1232698630227035 78-79 7.825664234554565E-5 80-81 1.565132846910913E-5 82-83 1.565132846910913E-5 84-85 0.0 86-87 0.1451973742079254 88-89 0.4358268925508128 90-91 0.3341089088300726 92-93 0.0 94-95 0.0 96-97 4.3823719713505563E-4 98-99 1.565132846910913E-5 100-101 2.660725839748552E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3194617.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.410672628148376 #Duplication Level Percentage of deduplicated Percentage of total 1 68.82717390468692 30.566610882016874 2 15.145677896188433 13.452594855580152 3 5.994725466635591 7.986893705831317 4 2.9914065029313224 5.314014995975885 5 1.7481074108932366 3.881731297200979 6 1.138287268825736 3.033126195156533 7 0.7793755095412732 2.422881342604367 8 0.5556508135088462 1.974146109944455 9 0.40914726942657087 1.6353454895283832 >10 2.2020504488324897 17.442571022668208 >50 0.1359014421493591 4.183854485500758 >100 0.06776402681530219 5.310441133686826 >500 0.003319490441542586 0.9878134803372876 >1k 0.0012006667549030378 1.0508280318338985 >5k 2.1188236851230077E-4 0.7571469721341554 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8821 0.27612073685202326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7842 0.24547543570950758 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7476 0.23401866327011972 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4855 0.15197439943504965 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3470 0.10862021957561735 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.130265693821826E-5 2 0.0 0.0 0.0 0.0 6.260531387643652E-5 3 0.0 0.0 0.0 0.0 6.260531387643652E-5 4 0.0 0.0 0.0 0.0 6.260531387643652E-5 5 0.0 0.0 0.0 0.0 6.260531387643652E-5 6 0.0 0.0 0.0 0.0 2.504212555057461E-4 7 6.260531387643652E-5 0.0 0.0 0.0 2.504212555057461E-4 8 6.260531387643652E-5 0.0 0.0 0.0 2.504212555057461E-4 9 6.260531387643652E-5 0.0 0.0 0.0 2.504212555057461E-4 10-11 6.260531387643652E-5 0.0 0.0 4.695398540732739E-5 2.504212555057461E-4 12-13 9.390797081465477E-5 1.565132846910913E-5 0.0 6.260531387643652E-5 3.2867789785129167E-4 14-15 1.5651328469109128E-4 3.130265693821826E-5 0.0 1.0955929928376391E-4 4.3823719713505563E-4 16-17 1.5651328469109128E-4 3.130265693821826E-5 0.0 1.5651328469109128E-4 5.321451679497104E-4 18-19 1.7216461316020042E-4 3.130265693821826E-5 0.0 1.7216461316020042E-4 5.477964964188196E-4 20-21 1.8781594162930955E-4 3.130265693821826E-5 0.0 2.3476992703663695E-4 7.1996110957902E-4 22-23 2.1911859856752782E-4 3.130265693821826E-5 0.0 4.225858686659465E-4 7.825664234554564E-4 24-25 2.504212555057461E-4 3.130265693821826E-5 0.0 6.417044672334742E-4 8.451717373318929E-4 26-27 2.504212555057461E-4 3.130265693821826E-5 0.0 0.001095592992837639 8.608230658010022E-4 28-29 3.5998055478951E-4 3.130265693821826E-5 0.0 0.0038971807888081734 8.764743942701113E-4 30-31 3.756318832586191E-4 3.130265693821826E-5 0.0 0.011895009636522938 8.921257227392204E-4 32-33 3.756318832586191E-4 3.130265693821826E-5 0.0 0.027624594747977613 0.0010642903358994208 34-35 3.912832117277282E-4 9.390797081465477E-5 0.0 0.05189980520356587 0.0010642903358994208 36-37 4.3823719713505563E-4 1.2521062775287301E-4 0.0 0.08902475633229273 0.001095592992837639 38-39 4.8519118254238306E-4 1.5651328469109128E-4 0.0 0.14367919534642182 0.0011581983067140756 40-41 5.164938394806013E-4 1.5651328469109128E-4 0.0 0.20526717287236623 0.0011895009636522938 42-43 5.790991533570378E-4 1.5651328469109128E-4 0.0 0.27012627804835443 0.0011895009636522938 44-45 7.1996110957902E-4 1.5651328469109128E-4 0.0 0.35235835782505387 0.001205152292121403 46-47 7.825664234554564E-4 1.5651328469109128E-4 0.0 0.4526051166696978 0.0012364549490596212 48-49 8.138690803936747E-4 1.5651328469109128E-4 0.0 0.5596915060553425 0.001330362919874276 50-51 8.138690803936747E-4 1.5651328469109128E-4 0.0 0.6745879083470726 0.00143992221915804 52-53 8.138690803936747E-4 1.5651328469109128E-4 0.0 0.7899381991644069 0.0015651328469109128 54-55 8.138690803936747E-4 1.8781594162930955E-4 0.0 0.9219884574582806 0.0016277381607873495 56-57 8.138690803936747E-4 1.8781594162930955E-4 0.0 1.0847309708800772 0.0016433894892564586 58-59 8.138690803936747E-4 1.8781594162930955E-4 0.0 1.260526692245111 0.0016746921461946768 60-61 8.295204088627838E-4 1.8781594162930955E-4 0.0 1.4513163862835512 0.0016903434746637859 62-63 8.451717373318929E-4 1.8781594162930955E-4 0.0 1.6478344665416857 0.0016903434746637859 64-65 8.764743942701113E-4 1.8781594162930955E-4 0.0 1.8643862472402795 0.0016903434746637859 66-67 8.764743942701113E-4 1.8781594162930955E-4 0.0 2.117468228585774 0.001705994803132895 68-69 9.077770512083295E-4 1.8781594162930955E-4 0.0 2.4066265220525653 0.0017216461316020043 70-71 9.70382365084766E-4 2.0346727009841868E-4 0.0 2.7133925600471045 0.0017372974600711134 72-73 9.70382365084766E-4 2.1911859856752782E-4 0.0 3.0266695506847925 0.0018781594162930956 74-75 0.0010016850220229843 2.1911859856752782E-4 0.0 3.3643782650627605 0.0019094620732313138 76-77 0.0010016850220229843 2.1911859856752782E-4 0.0 3.7347513019557588 0.001956416058638641 78-79 0.0010016850220229843 2.1911859856752782E-4 0.0 4.154864260723587 0.0019720673871077505 80-81 0.0011581983067140756 2.1911859856752782E-4 0.0 4.604871256867412 0.0020033700440459687 82-83 0.0011895009636522938 2.1911859856752782E-4 0.0 5.067336710472649 0.002112929343329732 84-85 0.001205152292121403 2.1911859856752782E-4 0.0 5.541024166590236 0.0021285806717988415 86-87 0.001220803620590512 2.1911859856752782E-4 0.0 6.048017649690088 0.0022381399710826055 88-89 0.001220803620590512 2.1911859856752782E-4 0.0 6.605877950314545 0.0023476992703663694 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9500 0.0 21.328932 2 GTACATG 10080 0.0 21.271324 1 GAGTACT 9645 0.0 20.9371 12-13 GGTATCA 15585 0.0 20.758959 1 GTATCAA 19700 0.0 20.55868 1 ACATGGG 9585 0.0 20.001108 3 TAGGACG 170 8.0201185E-5 19.539522 4 AGAGTAC 14350 0.0 19.512787 10-11 GTACTTT 10220 0.0 19.41085 14-15 AGTACTT 9925 0.0 19.079262 12-13 CAACGCA 22250 0.0 17.978868 5 ATCAACG 22385 0.0 17.934038 3 TATCAAC 22485 0.0 17.896486 2 TCAACGC 22515 0.0 17.76726 4 CATGGGG 6850 0.0 17.665031 4 AACGCAG 22855 0.0 17.544472 6 AGTACAT 7555 0.0 16.393467 2 GAGTACA 8555 0.0 16.151785 1 ACGCAGA 24790 0.0 15.907038 7 CGCAGAG 24705 0.0 15.8273115 8 >>END_MODULE