##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939045_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442877 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88864885918897 33.0 31.0 34.0 30.0 34.0 2 31.873930348879355 33.0 31.0 34.0 30.0 34.0 3 31.96514810340729 34.0 31.0 34.0 30.0 34.0 4 35.353583846717356 37.0 35.0 37.0 33.0 37.0 5 35.45741598209688 37.0 35.0 37.0 33.0 37.0 6 35.63583382367312 37.0 35.0 37.0 33.0 37.0 7 35.6245875428051 37.0 35.0 37.0 33.0 37.0 8 35.65012055774678 37.0 35.0 37.0 33.0 37.0 9 37.160994318989076 39.0 37.0 39.0 33.0 39.0 10-11 37.06637987853435 39.0 37.0 39.0 33.5 39.0 12-13 37.05229309220398 39.0 37.0 39.0 33.0 39.0 14-15 37.972332707500364 40.0 37.5 41.0 33.0 41.0 16-17 38.00347257597148 40.0 37.5 41.0 33.0 41.0 18-19 38.07986162368657 40.0 38.0 41.0 33.0 41.0 20-21 38.198116332854426 40.0 38.0 41.0 33.0 41.0 22-23 38.197655794638074 40.0 38.0 41.0 33.5 41.0 24-25 38.03997707358285 40.0 38.0 41.0 33.0 41.0 26-27 37.75518287421589 40.0 37.5 41.0 32.5 41.0 28-29 37.71310617606352 40.0 37.0 41.0 33.0 41.0 30-31 37.757581207545755 40.0 37.0 41.0 33.0 41.0 32-33 37.572913699504525 40.0 37.0 41.0 33.0 41.0 34-35 37.176036488210705 40.0 36.0 41.0 31.0 41.0 36-37 36.78644333508677 39.5 35.5 41.0 30.5 41.0 38-39 36.46720233256196 39.0 35.0 40.5 30.0 41.0 40-41 36.55806593354804 39.0 35.0 40.5 30.0 41.0 42-43 36.4698231380776 39.0 35.0 41.0 30.0 41.0 44-45 36.50226977074276 39.0 36.0 41.0 30.0 41.0 46-47 36.285449140848456 39.0 36.0 41.0 30.0 41.0 48-49 35.996098073501756 39.0 35.5 41.0 28.0 41.0 50-51 34.75881520046407 38.0 34.0 40.0 26.0 40.5 52-53 34.982925433006415 38.0 34.5 39.5 27.5 40.5 54-55 35.65500385687761 39.0 35.0 40.0 28.0 41.0 56-57 35.56275864124247 39.0 35.0 40.5 27.5 41.0 58-59 35.442611185846054 39.0 35.0 40.0 27.0 41.0 60-61 34.84523455568285 38.0 34.0 40.0 26.0 41.0 62-63 34.56077926254282 38.0 34.0 40.0 26.0 41.0 64-65 34.08192867444696 37.5 33.5 39.5 25.0 41.0 66-67 33.41703658731825 37.0 33.0 39.0 22.0 40.5 68-69 32.92902686784805 36.0 32.5 39.0 22.0 39.5 70-71 32.13804087250681 35.5 31.5 37.5 21.5 39.0 72-73 31.52616404586115 35.0 31.0 37.0 19.5 39.0 74-75 31.125598023947987 35.0 31.0 37.0 18.0 37.5 76-77 30.682152394140317 34.5 31.0 36.0 15.5 37.0 78-79 30.19130875327557 34.0 30.5 35.5 9.0 36.5 80-81 29.671706597305246 34.0 29.0 35.0 4.5 36.0 82-83 29.161752872906007 34.0 29.0 35.0 2.0 36.0 84-85 28.690633366530896 34.0 29.0 35.0 2.0 35.0 86-87 28.449655791865837 34.0 29.0 35.0 2.0 35.0 88-89 27.79070807837397 34.0 27.0 35.0 2.0 35.0 90-91 27.149390072750485 33.0 25.5 35.0 2.0 35.0 92-93 26.640579896969733 33.0 25.0 35.0 2.0 35.0 94-95 26.023062949925738 33.0 24.0 35.0 2.0 35.0 96-97 25.12706696412792 32.0 19.5 34.0 2.0 35.0 98-99 24.358177793394724 32.0 7.5 34.0 2.0 35.0 100-101 21.122013172293965 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1877877439089808 1101 2 0.23615572042379185 1101 3 0.35976663569409695 1101 4 0.22388128773843619 1101 5 0.16093842618241894 1101 6 0.2912200770246045 1101 7 0.34370268357320555 1101 8 0.338563832432591 1101 9 0.38576404090071037 1101 10-11 0.3347836834172071 1101 12-13 0.4078884557636755 1101 14-15 0.5790566200445113 1101 16-17 0.5166626754345245 1101 18-19 0.5737115710235372 1101 20-21 0.6014047235173408 1101 22-23 0.6700286748129187 1101 24-25 0.645644929763634 1101 26-27 0.5215155942262442 1101 28-29 0.5114593940011503 1101 30-31 0.6406313840040454 1101 32-33 0.5978329440669157 1101 34-35 0.46835691321580697 1101 36-37 0.5596872886901068 1101 38-39 0.782944660854902 1101 40-41 0.5582868342231038 1101 42-43 0.5828429064680805 1101 44-45 0.5679047623612732 1101 46-47 0.5385293003092713 1101 48-49 0.690624737234927 1101 50-51 0.6516191114459602 1101 52-53 0.6002037417140613 1101 54-55 0.7920221561349976 1101 56-57 0.9670165656464746 1101 58-59 0.8283417432026781 1101 60-61 0.8001642200072183 1101 62-63 0.7363456265610395 1101 64-65 0.6668948567640811 1101 66-67 0.7693561963723923 1101 68-69 0.5764129165060368 1101 70-71 0.5649574379388511 1101 72-73 0.5351866236229554 1101 74-75 0.3562112098669594 1101 76-77 0.37089684016871516 1101 78-79 0.4579531776883208 1101 80-81 0.274263755017472 1101 82-83 0.1740933469072914 1101 84-85 0.21526556602708524 1101 86-87 0.044653511712578364 1101 88-89 0.13321189743485817 1101 90-91 0.20770384737370406 1101 92-93 0.1440558662778031 1101 94-95 -0.04455585168581777 1101 96-97 0.00972158212210772 1101 98-99 0.07539542825028889 1101 100-101 0.26036621770270685 1102 1 0.17545321022785387 1102 2 0.23650903034098647 1102 3 0.30844512071535135 1102 4 0.21537585367829593 1102 5 0.24778826651350983 1102 6 0.25419756976491215 1102 7 0.3645237450981682 1102 8 0.3332009660138695 1102 9 0.28105314353488353 1102 10-11 0.36230345370667294 1102 12-13 0.2993951334983578 1102 14-15 0.5202594689749844 1102 16-17 0.5136019362605566 1102 18-19 0.27828894323670994 1102 20-21 0.43016994080588944 1102 22-23 0.45449130992589204 1102 24-25 0.5843743138641031 1102 26-27 0.6101813175514934 1102 28-29 0.5551079392564304 1102 30-31 0.5503347041128777 1102 32-33 0.33865028343054604 1102 34-35 0.4816600950729324 1102 36-37 0.5236925115323601 1102 38-39 0.5868617925455624 1102 40-41 0.5856810554199186 1102 42-43 0.45391403224464 1102 44-45 0.6751222104356387 1102 46-47 0.7868512259074052 1102 48-49 0.7638364011887475 1102 50-51 0.7666622560223928 1102 52-53 0.8013951287508974 1102 54-55 0.8489945619214865 1102 56-57 0.9444487469807257 1102 58-59 0.9164441203639626 1102 60-61 0.9034527200057383 1102 62-63 0.9003815720047896 1102 64-65 0.67562421132358 1102 66-67 0.7525928815218563 1102 68-69 0.8378714129301059 1102 70-71 0.7238435073069311 1102 72-73 0.729520371514468 1102 74-75 0.6763495797375398 1102 76-77 0.5811954106815165 1102 78-79 0.4771326479006923 1102 80-81 0.6577013187151728 1102 82-83 0.6740233348250371 1102 84-85 0.3166253365528853 1102 86-87 0.35098798040359114 1102 88-89 0.34662851658981 1102 90-91 0.5071180500778851 1102 92-93 0.47423786815069846 1102 94-95 0.4417073051205662 1102 96-97 0.2309435145146601 1102 98-99 0.2349139696357021 1102 100-101 0.4770417958932036 1103 1 0.17365658642435378 1103 2 0.17896737109042604 1103 3 0.32966596964176986 1103 4 0.2773567744465453 1103 5 -8.86119991605483E-6 1103 6 0.14354484460616845 1103 7 0.17037568804077097 1103 8 -0.02625670670819602 1103 9 0.2410726295929848 1103 10-11 0.2644212622994999 1103 12-13 0.2900496847526952 1103 14-15 0.36414043065703083 1103 16-17 0.3544955557900664 1103 18-19 0.39952955560284664 1103 20-21 0.4211232551693982 1103 22-23 0.5156660903899066 1103 24-25 0.38866858570270324 1103 26-27 0.42428478551507 1103 28-29 0.38635936950535665 1103 30-31 0.376940859210805 1103 32-33 0.41623045249463786 1103 34-35 0.4282467987854588 1103 36-37 0.3387777948198831 1103 38-39 0.4982562823598755 1103 40-41 0.3758109509163958 1103 42-43 0.286059729246837 1103 44-45 0.5329326409087827 1103 46-47 0.5213557067761627 1103 48-49 0.5197059116333236 1103 50-51 0.4426866031932164 1103 52-53 0.49391327991000367 1103 54-55 0.5823623560673425 1103 56-57 0.30847628172854513 1103 58-59 0.4904983646493193 1103 60-61 0.47195702057645406 1103 62-63 0.6389736754126503 1103 64-65 0.5168362418263115 1103 66-67 0.44720237444516187 1103 68-69 0.31253838163451775 1103 70-71 0.3232189363816147 1103 72-73 0.4047946325814138 1103 74-75 0.3812115014835662 1103 76-77 0.44396750470463786 1103 78-79 0.14988793722126914 1103 80-81 0.23073618558355946 1103 82-83 0.23142254706126764 1103 84-85 0.16893118676219032 1103 86-87 0.37622421707482445 1103 88-89 0.3994902279881174 1103 90-91 0.47335695192409233 1103 92-93 0.4178089136713332 1103 94-95 0.2321833497512671 1103 96-97 0.05941159367012716 1103 98-99 -0.16291453687236412 1103 100-101 0.4149580127778876 1104 1 0.24178257482116194 1104 2 0.4333613080984726 1104 3 0.4042127411975365 1104 4 0.31288619520945815 1104 5 0.21979990440353703 1104 6 0.26921436745374194 1104 7 0.24679464906278525 1104 8 0.3348132251078937 1104 9 0.3497040472830122 1104 10-11 0.47095148615239424 1104 12-13 0.4356597969378484 1104 14-15 0.6863600408996859 1104 16-17 0.633936283475073 1104 18-19 0.6496836498995577 1104 20-21 0.6563448247716579 1104 22-23 0.6176613303383434 1104 24-25 0.5460002686148755 1104 26-27 0.5379298917276643 1104 28-29 0.6772781539375501 1104 30-31 0.6625442339883776 1104 32-33 0.786956585312673 1104 34-35 0.7096443553223182 1104 36-37 0.872285430923391 1104 38-39 0.9079474638919507 1104 40-41 0.8361848151739437 1104 42-43 0.9223513543905284 1104 44-45 1.0038934582159342 1104 46-47 0.9750349049459217 1104 48-49 0.968547729901033 1104 50-51 0.8961802133413883 1104 52-53 0.9105749788164985 1104 54-55 1.0383749018176047 1104 56-57 1.1524338731784525 1104 58-59 1.0409993960688126 1104 60-61 1.0455047099493484 1104 62-63 0.9590410269928498 1104 64-65 1.0493498572384397 1104 66-67 0.7733482961202682 1104 68-69 0.724890220530618 1104 70-71 0.9317901785616058 1104 72-73 0.935020453836529 1104 74-75 0.8768243131308431 1104 76-77 0.9587158473473636 1104 78-79 0.9745356574130213 1104 80-81 1.0155977706627297 1104 82-83 1.1481320489430153 1104 84-85 0.9679899742987779 1104 86-87 1.1034894032364804 1104 88-89 1.1140325116880874 1104 90-91 1.116741413820062 1104 92-93 0.8940123113943379 1104 94-95 0.9224462211241473 1104 96-97 0.8537634753077121 1104 98-99 0.850870435221946 1104 100-101 1.210314038089841 1105 1 0.1676652334638895 1105 2 0.17908476173992582 1105 3 0.21721237217208866 1105 4 0.3214469304653633 1105 5 0.24961017291867904 1105 6 0.3282557092215228 1105 7 0.27568617523411376 1105 8 0.3099323941237273 1105 9 0.2901419210850449 1105 10-11 0.20580124475734607 1105 12-13 0.20784854362698724 1105 14-15 0.391098196602222 1105 16-17 0.3653597281638383 1105 18-19 0.5251962458953017 1105 20-21 0.505457362247391 1105 22-23 0.40348382036483343 1105 24-25 0.40419405282888476 1105 26-27 0.3416916039183846 1105 28-29 0.2699122750346703 1105 30-31 0.25840841952301474 1105 32-33 0.28188903618752903 1105 34-35 0.48291097293115826 1105 36-37 0.45804890963237455 1105 38-39 0.6646442385222215 1105 40-41 0.5218128025877817 1105 42-43 0.41032991379379524 1105 44-45 0.5394157056464053 1105 46-47 0.5692854230008777 1105 48-49 0.5640330638690614 1105 50-51 0.4383331820309948 1105 52-53 0.5379812867496696 1105 54-55 0.5895198633101231 1105 56-57 0.7912630471510198 1105 58-59 0.6830788326171771 1105 60-61 0.7654236772021576 1105 62-63 0.6827504205464763 1105 64-65 0.6407608991785096 1105 66-67 0.7981461509687975 1105 68-69 0.7729312194759501 1105 70-71 0.6190765968967114 1105 72-73 0.4793520919819727 1105 74-75 0.5740776938624741 1105 76-77 0.27958183433758066 1105 78-79 0.23069035768356727 1105 80-81 0.5417704636532115 1105 82-83 0.5924209434606951 1105 84-85 0.6391981286693245 1105 86-87 0.6805993444326042 1105 88-89 0.5723645712649557 1105 90-91 0.7433875620896799 1105 92-93 0.7333339378139421 1105 94-95 0.6089552840833825 1105 96-97 0.7321652239205925 1105 98-99 0.6823944884751576 1105 100-101 1.1437944720987048 1106 1 0.08349761545098389 1106 2 0.15500367733252673 1106 3 0.20596066929767787 1106 4 0.1959541048184832 1106 5 0.14192060745403268 1106 6 0.20180906170909907 1106 7 0.15379325791847975 1106 8 0.08960060416400495 1106 9 0.08929929002206904 1106 10-11 0.06779414891681768 1106 12-13 0.1947512644065128 1106 14-15 0.18169981523474377 1106 16-17 0.2510130377877857 1106 18-19 0.36289321700691346 1106 20-21 0.2753714440108297 1106 22-23 0.38303288224548027 1106 24-25 0.27031205088516685 1106 26-27 0.2228898545730047 1106 28-29 0.3060344321362436 1106 30-31 0.23985080189206798 1106 32-33 0.4680752765379097 1106 34-35 0.30097820517139695 1106 36-37 0.36362903279103875 1106 38-39 0.3728272662223091 1106 40-41 0.4929509380623145 1106 42-43 0.30945449841976114 1106 44-45 0.36283293427931085 1106 46-47 0.34807089689862636 1106 48-49 0.4179115857952951 1106 50-51 0.2526466637036151 1106 52-53 0.28639352877215885 1106 54-55 0.3356048436395369 1106 56-57 0.3575939840820723 1106 58-59 0.5006182268394994 1106 60-61 0.7005701562308104 1106 62-63 0.664159382015896 1106 64-65 0.3785208925579866 1106 66-67 0.3604596835685001 1106 68-69 0.40158682930657363 1106 70-71 0.23667572434599293 1106 72-73 0.3818458215846121 1106 74-75 0.5442004705769143 1106 76-77 0.36564118136747226 1106 78-79 0.5472186370695553 1106 80-81 0.4179746770207231 1106 82-83 0.4651292246291341 1106 84-85 0.5564087248981728 1106 86-87 0.4321345838535642 1106 88-89 0.780214450949714 1106 90-91 0.8145592245619362 1106 92-93 0.8016876349081237 1106 94-95 0.6212966143837235 1106 96-97 1.0136063331125058 1106 98-99 1.2497317658283045 1106 100-101 1.3522899586857804 1107 1 0.0011968986025401307 1107 2 0.09244463359343413 1107 3 0.11738714560818053 1107 4 0.16929263314978016 1107 5 0.15944198064927662 1107 6 0.17704771331095515 1107 7 0.10034826957658538 1107 8 0.04703651521720076 1107 9 0.14390498252789286 1107 10-11 0.17518226326532726 1107 12-13 0.26499888810347016 1107 14-15 0.3573769607501802 1107 16-17 0.09105416542234934 1107 18-19 0.10297999662223134 1107 20-21 0.2067875133037944 1107 22-23 0.19588759149751667 1107 24-25 0.1715891318546312 1107 26-27 0.14802137210816824 1107 28-29 0.0636785856698765 1107 30-31 0.18719885075500287 1107 32-33 0.21858618041648725 1107 34-35 0.2428949960107758 1107 36-37 0.2313815255219609 1107 38-39 0.5988586997047207 1107 40-41 0.25642363419476766 1107 42-43 0.15040202723821494 1107 44-45 -0.030821951044345042 1107 46-47 0.3566852826854401 1107 48-49 0.48060901529260036 1107 50-51 0.5218901991482383 1107 52-53 0.63429992076437 1107 54-55 0.5743955972240329 1107 56-57 0.5600811999955084 1107 58-59 0.5720653639905109 1107 60-61 0.6142529331784985 1107 62-63 0.6144441707146271 1107 64-65 0.6831222445511003 1107 66-67 0.497950785517439 1107 68-69 0.6414330934528181 1107 70-71 0.3635353787186233 1107 72-73 0.4915670891777353 1107 74-75 0.32029869689022306 1107 76-77 0.27556328703699506 1107 78-79 0.4313266923462429 1107 80-81 0.386511066347353 1107 82-83 0.5254128184102491 1107 84-85 0.7264523955625393 1107 86-87 0.6217092850986283 1107 88-89 0.5968165546174973 1107 90-91 0.4349910902572809 1107 92-93 0.5356014394747497 1107 94-95 0.6889542286132553 1107 96-97 0.7634102440676536 1107 98-99 0.7125907645281941 1107 100-101 0.7734682904378047 1108 1 0.24219691852617942 1108 2 0.1993522644524255 1108 3 0.24882444748593358 1108 4 0.2370718079126064 1108 5 0.3133746450376549 1108 6 0.3756177202632571 1108 7 0.20024487637096655 1108 8 0.2828139199218995 1108 9 0.44880798061627303 1108 10-11 0.3467565244390869 1108 12-13 0.3892010249662903 1108 14-15 0.4887122850493597 1108 16-17 0.4203575930224588 1108 18-19 0.3564018817970549 1108 20-21 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2316 90-91 -2.84036063785177 2316 92-93 -2.975247370682677 2316 94-95 -3.072872627781013 2316 96-97 -3.2933638790706787 2316 98-99 -3.4721165301676145 2316 100-101 -3.0977602708334757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4345.0 3 900.0 4 823.0 5 831.0 6 1015.0 7 1248.0 8 1531.0 9 1913.0 10 2548.0 11 3573.0 12 5201.0 13 7931.0 14 6803.0 15 6874.0 16 8377.0 17 10934.0 18 10043.0 19 8253.0 20 8588.0 21 9449.0 22 10306.0 23 12009.0 24 14168.0 25 16311.0 26 18990.0 27 22662.0 28 27047.0 29 32619.0 30 39362.0 31 48144.0 32 58893.0 33 75771.0 34 100324.0 35 147104.0 36 213324.0 37 291122.0 38 194141.0 39 19396.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.397353608614395 17.531369582628816 13.605513593485805 16.46576321527098 2 12.922207601314911 27.352164476189618 32.96411881363939 26.761509108856085 3 32.316313866359756 23.982789755159153 30.832963918008527 12.867932460472561 4 11.239465992508688 14.683679720383545 44.82889240552434 29.24796188158343 5 9.978441980340364 43.57190088723012 34.68260031209518 11.76705682033434 6 24.183459772824985 38.22198798940405 15.986644106347864 21.607908131423102 7 22.764489806391126 37.1032245492642 21.722487241952496 18.409798402392184 8 20.914327877333267 23.506800525096374 20.682292421538936 34.896579176031416 9 18.421180380255983 26.978812407418708 28.559282540070015 26.040724672255294 10-11 24.970475382745143 21.959172464679682 26.937269116799595 26.133083035775584 12-13 35.59149019976332 21.794149434322343 23.589810624802578 19.024549741111755 14-15 25.957606450221522 25.528185739973193 33.08678771118242 15.427420098622871 16-17 17.61695039607002 28.49720847436588 33.03687892802087 20.848962201543227 18-19 16.428710131559786 37.03210280665497 28.139091356723277 18.40009570506197 20-21 21.834592035275787 27.599087451205722 23.043313471746046 27.523007041772445 22-23 18.057142738233182 27.59527922642518 26.21271642861528 28.134861606726364 24-25 26.52311250452205 27.025004049470482 25.08646096397168 21.365422482035786 26-27 25.445840045920164 20.735381589968316 34.90983312321275 18.908945240898774 28-29 17.871554682018537 33.13911123273114 29.690073551215846 19.299260534034477 30-31 19.695292962592912 28.378725648874926 26.985033072757986 24.94094831577418 32-33 21.0777543167078 28.75993892646937 28.44219150359412 21.720115253228713 34-35 26.115935247684526 24.235118748616 27.048382773828894 22.60056322987058 36-37 23.808691352177714 22.916467880163953 34.0529114532367 19.221929314421626 38-39 23.175705079777327 26.10774377952701 32.2175067975226 18.49904434317306 40-41 20.130189732686734 29.320188354280923 30.748613588296635 19.8010083247357 42-43 17.58310860569047 31.385186951927146 24.87507812513601 26.156626317246374 44-45 21.45980719488193 25.847439920771887 25.570670834221303 27.122082050124874 46-47 25.479771829383203 29.08740110157576 25.357796235644454 20.07503083339659 48-49 23.777206468257727 26.39201553540881 28.07688717882994 21.753890817503525 50-51 17.62841720355215 28.827581403447677 34.55716524464565 18.98683614835452 52-53 20.648068373591947 32.12360152365404 26.442703824249055 20.785626278504957 54-55 16.196277488699483 25.861393544262405 30.79737207458394 27.144956892454168 56-57 23.482043822862 25.79796141321036 26.470295385502347 24.249699378425294 58-59 28.205808303633244 24.576741097021916 25.57249902276003 21.64495157658481 60-61 21.642187537511994 25.741445700382137 33.44912686872778 19.167239893378092 62-63 21.56084591606437 25.959259875671037 31.172281339722247 21.307612868542346 64-65 19.670288758536458 32.626897140415295 28.422410139303278 19.28040396174497 66-67 20.844812873339023 26.161717838421485 26.18536772261019 26.8081015656293 68-69 17.556834431129694 28.836182587761876 28.668630612686368 24.938352368422063 70-71 24.88671359168684 27.7750162758319 24.372609786538415 22.96566034594285 72-73 22.096274952274904 22.583365231332184 31.98955394233144 23.330805874061475 74-75 18.454901988102037 33.259748944648 27.094821460913664 21.190527606336293 76-77 22.052533657497378 24.744158448525436 27.00152552565519 26.201782368321997 78-79 21.40791387119044 25.773330469243117 30.51290076519253 22.30585489437391 80-81 24.404368354186666 26.54787979818975 27.64929916165396 21.39845268596962 82-83 21.274212435361253 24.811041243097396 31.711852998844332 22.202893322697022 84-85 20.600282257179956 26.753878772874202 29.758423928277566 22.887415041668277 86-87 21.375915729392236 28.347176128899044 28.346479723638534 21.930428418070182 88-89 21.339630144742625 26.948990116717585 25.72390464523327 25.987475093306518 90-91 22.058384093782323 22.754054741182657 28.79783932881278 26.38972183622224 92-93 24.23165362086344 28.30142875751524 28.465299463702205 19.00161815791912 94-95 22.35874247029393 26.995132641950676 28.065366243092036 22.580758644663355 96-97 16.703294636550698 26.646412734413204 31.989765994290913 24.66052663474519 98-99 21.845421884605404 28.984833365550895 25.222344707003053 23.94740004284065 100-101 21.547531370408482 24.32870721010121 28.043116791765254 26.080644627725054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7845.0 1 4602.0 2 1340.0 3 1228.5 4 896.0 5 835.0 6 1502.5 7 2634.5 8 3558.5 9 3523.5 10 2598.0 11 1819.0 12 1657.5 13 1646.0 14 1625.5 15 1901.5 16 2586.0 17 2492.5 18 1805.0 19 1673.0 20 1853.5 21 2132.0 22 2475.5 23 2918.5 24 3395.0 25 4115.0 26 5588.5 27 6998.0 28 7533.5 29 8189.0 30 9399.0 31 10710.0 32 12839.0 33 15809.0 34 18959.0 35 21120.0 36 22965.5 37 25015.0 38 23784.5 39 21185.0 40 19730.0 41 18646.5 42 17826.0 43 17681.5 44 19576.0 45 23082.0 46 45203.5 47 130266.5 48 320255.0 49 315906.0 50 128649.0 51 48615.0 52 20311.0 53 11987.5 54 8444.5 55 6163.0 56 4525.0 57 3370.5 58 2617.5 59 2001.0 60 1660.5 61 1443.0 62 1067.5 63 659.5 64 380.5 65 289.0 66 234.5 67 213.5 68 201.0 69 162.5 70 125.5 71 107.0 72 99.5 73 96.5 74 82.0 75 69.5 76 70.0 77 60.5 78 39.5 79 29.5 80 25.0 81 17.0 82 11.5 83 9.5 84 7.0 85 5.5 86 5.0 87 3.5 88 3.0 89 2.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3147184410036337 2 0.08718691891270011 3 0.09772142739817741 4 0.10215700991837834 5 0.114354861848931 6 0.05662298310944037 7 0.058632856438906436 8 0.05932591620768783 9 0.06244468516720413 10-11 0.0596031401152004 12-13 0.05859820345046736 14-15 0.056692289086318515 16-17 0.057801184716368754 18-19 0.06563276010359857 20-21 0.06802381630589441 22-23 0.0836176611034759 24-25 0.09221160223636526 26-27 0.08625128822484522 28-29 0.08514239259479499 30-31 0.07841971283761541 32-33 0.06989507768160419 34-35 0.31641643743714815 36-37 0.4908249282509874 38-39 0.4637955972685129 40-41 0.44598396121083084 42-43 0.4767904679331641 44-45 0.48673587561517717 46-47 0.48056764367302274 48-49 0.49824066777694836 50-51 0.49394369721050374 52-53 0.49331994341860047 54-55 0.4845527373435157 56-57 0.46337976140724396 58-59 0.44588000224551366 60-61 0.4045043340492641 62-63 0.47460732966150265 64-65 0.49876046260353446 66-67 0.5095375420080852 68-69 0.5198987855513671 70-71 0.5174730763606323 72-73 0.5067306499445206 74-75 0.5084979523549131 76-77 0.4836517596440999 78-79 0.4645233100257333 80-81 0.4695826463378375 82-83 0.4792854831007771 84-85 0.4818498042452683 86-87 0.4803943787308274 88-89 0.48638934573078646 90-91 0.49983470524514567 92-93 0.5055177953491532 94-95 0.5125870049907234 96-97 0.5039237578809559 98-99 0.5076316276439363 100-101 0.4858002449273223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1442877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.200096027590426 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8092573135859 12.867264095463993 2 11.943330969741707 4.108528791377042 3 4.492925680669686 2.3183625945703707 4 2.262558078926375 1.5566486490213673 5 1.3329046228621455 1.1463043754424056 6 0.8622678059312898 0.8898653438110736 7 0.5918979213356829 0.7126490759853438 8 0.4559093205825696 0.6273347275114965 9 0.34467312903549313 0.5335569825786494 >10 2.3639254878733045 8.038458595552186 >50 0.294185891835848 3.4988445798649597 >100 0.19329909750818192 6.459202482269129 >500 0.019773811644887087 2.338300986725294 >1k 0.018159622939182017 7.456145520512285 >5k 0.005246113293541472 6.35231030940173 >10k+ 0.009685132234230408 41.09622288991269 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 97145 6.73272912382691 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 56271 3.899916624909815 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 40164 2.783605255333615 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 33253 2.3046316491287895 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 31901 2.210929968389544 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 26976 1.8695980322647046 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 25303 1.7536491329475763 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 24647 1.7081844121155165 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 23211 1.6086610293185075 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 22711 1.5740080408794372 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 22494 1.5589686438968808 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 22372 1.550513314717748 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 20627 1.4295743850653935 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 18726 1.2978237230200496 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 16383 1.1354398191945674 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 14766 1.0233720545826153 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 13715 0.95053147288369 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 13195 0.9144923649070572 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 12857 0.8910669447222458 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 12723 0.8817799438205751 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11307 0.7836426805611288 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10894 0.7550193121104571 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10343 0.7168317188506019 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 10091 0.6993666126773107 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9194 0.6371991514176191 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8964 0.621258776735647 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8531 0.5912492887474123 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8182 0.5670615028169415 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 8159 0.5654674653487443 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7926 0.5493191727361376 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6958 0.4822309871180981 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6439 0.44626118511834345 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5641 0.39095501556958767 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5563 0.3855491493730928 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5450 0.3777175739858629 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5330 0.3694008567604862 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5181 0.3590742662056433 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4825 0.33440133843702546 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4744 0.32878755430989615 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4675 0.3240054419053045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4648 0.3221341805295947 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4288 0.2971840288534643 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4125 0.2858871546223275 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 4052 0.28082781831022324 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4001 0.27729321348943814 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3463 0.24000659792899878 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3270 0.22663054439151778 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3221 0.2232345515244889 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.2226801037094638 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3091 0.21422477453033073 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3007 0.20840307247256695 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2974 0.20611597523558833 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2666 0.1847697343571212 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2655 0.18400736861146166 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2508 0.1738193900103751 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2458 0.1703540911664681 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2456 0.17021547921271182 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2305 0.15975027670411268 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2173 0.1506018877561982 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 2046 0.14180002869267444 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2012 0.13944362547881767 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1879 0.13022593055402504 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1849 0.12814675124768085 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1778 0.1232260268893329 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1760 0.1219785193055264 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1652 0.11449347380268728 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1552 0.10756287611487327 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7326494219535E-4 0.0 12-13 0.0 0.0 0.0 2.7722390751256E-4 0.0 14-15 0.0 0.0 0.0 5.5444781502512E-4 0.0 16-17 0.0 0.0 0.0 0.0010395896531721 0.0 18-19 0.0 0.0 0.0 0.00159403746819722 0.0 20-21 0.0 0.0 0.0 0.00249501516761304 0.0 22-23 0.0 0.0 0.0 0.00460884746239631 0.0 24-25 0.0 0.0 0.0 0.007277127572204699 0.0 26-27 0.0 0.0 0.0 0.010084019635769369 0.0 28-29 0.0 0.0 0.0 0.02602439431774157 0.0 30-31 0.0 0.0 0.0 0.060989259652763195 0.0 32-33 0.0 0.0 0.0 0.14235447650769956 0.0 34-35 0.0 0.0 0.0 0.27514472820621577 0.0 36-37 0.0 0.0 0.0 0.4324346427311545 0.0 38-39 0.0 0.0 0.0 0.6313774493598554 0.0 40-41 0.0 0.0 0.0 0.8398498278093005 0.0 42-43 0.0 0.0 0.0 1.0602774872702247 0.0 44-45 0.0 0.0 0.0 1.3302935731874581 0.0 46-47 0.0 0.0 0.0 1.6895411043352966 0.0 48-49 0.0 0.0 0.0 2.010843613142354 0.0 50-51 0.0 0.0 0.0 2.350581511798996 0.0 52-53 0.0 0.0 0.0 2.64814672352529 0.0 54-55 0.0 0.0 0.0 2.9963399513610653 0.0 56-57 0.0 0.0 0.0 3.5153377592130166 0.0 58-59 0.0 0.0 0.0 3.9924054510536937 0.0 60-61 0.0 0.0 0.0 4.613802839743096 0.0 62-63 0.0 0.0 0.0 5.1571270454792755 0.0 64-65 0.0 0.0 0.0 5.610596052192944 0.0 66-67 0.0 0.0 0.0 6.179979305235304 0.0 68-69 0.0 0.0 0.0 6.877336044583149 0.0 70-71 0.0 0.0 0.0 7.573306664393431 0.0 72-73 0.0 0.0 0.0 8.233411441169276 0.0 74-75 0.0 0.0 0.0 9.04408345271288 0.0 76-77 0.0 0.0 0.0 9.663574927038134 0.0 78-79 0.0 0.0 0.0 10.709471424106145 0.0 80-81 0.0 0.0 0.0 11.764620269087386 0.0 82-83 0.0 0.0 0.0 12.565658749844928 0.0 84-85 0.0 0.0 0.0 13.664608972213156 0.0 86-87 0.0 0.0 0.0 14.785182659367361 0.0 88-89 0.0 0.0 0.0 15.812401195666713 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAACGA 25 5.363264E-5 75.748245 6 GTAAGCG 505 0.0 40.499733 94-95 AGTCCCG 30 0.0040111905 31.570528 22-23 GTGTAAC 80 4.513032E-4 29.666307 1 CATATAG 220 1.8189894E-12 27.98782 3 GCTTTGC 1555 0.0 27.777584 1 CTTTGCG 1560 0.0 27.325388 2 TCCCGTA 35 0.008319167 27.192488 30-31 AACGGAC 35 0.0085276915 27.054823 14-15 TTTGCGT 1590 0.0 26.809814 3 CGCCGTT 45 8.881128E-4 26.424246 94-95 CGTATAG 110 9.524383E-5 25.890594 1 TTGCGTT 1675 0.0 25.449314 4 CATTCCG 190 1.2343662E-8 24.917189 9 TTAGGCA 210 1.3096724E-9 24.809717 4 GCATGAT 120 1.7194124E-4 23.733046 1 GACATAT 275 5.2750693E-11 22.438513 1 ATATGAG 380 0.0 22.435581 3 GGTTTGC 215 4.5816705E-8 22.019838 8 TTACTCG 65 2.9164707E-4 21.95245 94-95 >>END_MODULE