##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939045_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442877 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0070241607566 33.0 31.0 34.0 30.0 34.0 2 32.21059106216261 34.0 31.0 34.0 30.0 34.0 3 32.32873904012608 34.0 31.0 34.0 30.0 34.0 4 35.761140415988336 37.0 35.0 37.0 35.0 37.0 5 35.72426686405009 37.0 35.0 37.0 33.0 37.0 6 35.85951124038986 37.0 35.0 37.0 35.0 37.0 7 35.8324216132075 37.0 35.0 37.0 35.0 37.0 8 35.86185586158765 37.0 35.0 37.0 35.0 37.0 9 37.41593635493531 39.0 37.0 39.0 34.0 39.0 10-11 37.29889519342258 39.0 37.0 39.0 33.5 39.0 12-13 37.277742662749496 39.0 37.0 39.0 33.5 39.0 14-15 38.20752669839494 40.0 37.5 41.0 33.5 41.0 16-17 38.49368934427536 40.0 38.0 41.0 34.0 41.0 18-19 38.684754833572086 40.0 38.0 41.0 34.0 41.0 20-21 38.63587228849029 40.0 38.0 41.0 34.0 41.0 22-23 38.61603587831811 40.0 38.0 41.0 34.0 41.0 24-25 38.4287995442439 40.0 38.0 41.0 34.0 41.0 26-27 38.06935241188265 40.0 37.5 41.0 33.5 41.0 28-29 37.983729382338204 40.0 37.0 41.0 33.0 41.0 30-31 38.033080782353586 40.0 37.0 41.0 33.0 41.0 32-33 37.970373427534014 40.0 37.0 41.0 33.0 41.0 34-35 37.764356906375255 40.0 37.0 41.0 33.0 41.0 36-37 37.53690404656807 39.5 36.5 41.0 32.0 41.0 38-39 37.31233847375764 39.0 36.0 41.0 31.5 41.0 40-41 37.391605798692474 40.0 36.5 41.0 32.0 41.0 42-43 37.27005385767463 40.0 36.0 41.0 31.5 41.0 44-45 37.128725109624725 40.0 36.0 41.0 31.0 41.0 46-47 36.891432533750276 39.0 36.0 41.0 31.0 41.0 48-49 36.54339281865329 39.0 36.0 41.0 30.0 41.0 50-51 36.289733636339065 39.0 35.0 40.0 30.0 41.0 52-53 36.10210503043572 39.0 35.0 40.0 28.5 41.0 54-55 35.98210242453099 39.0 35.0 40.0 28.0 41.0 56-57 35.83571988464713 38.5 35.0 40.0 28.0 41.0 58-59 35.61313611624553 38.0 35.0 40.0 28.0 41.0 60-61 34.975027670411265 38.0 34.0 40.0 26.5 41.0 62-63 34.62569262660642 37.5 34.0 40.0 26.0 41.0 64-65 34.136739652790915 37.0 33.0 39.5 26.0 40.5 66-67 33.507712715636885 36.0 32.0 39.0 25.5 40.0 68-69 32.93272295559497 36.0 32.0 38.0 24.5 39.0 70-71 32.10399743013438 35.0 31.5 37.0 22.5 39.0 72-73 31.41191106379823 34.5 30.5 36.5 21.5 38.5 74-75 30.959672931233918 34.0 30.0 36.0 20.0 37.0 76-77 29.001152558395482 31.5 27.5 34.0 18.0 36.0 78-79 29.982637466672486 34.0 29.0 35.0 18.0 36.0 80-81 29.92794361542945 34.0 29.5 35.0 18.0 36.0 82-83 29.490754236154572 33.5 29.0 35.0 13.0 35.5 84-85 29.036318757593335 33.0 29.0 35.0 7.0 35.0 86-87 28.551449291935487 33.0 29.0 35.0 2.0 35.0 88-89 27.712487620219882 32.5 26.5 34.5 2.0 35.0 90-91 26.987786900754536 32.0 25.0 34.0 2.0 35.0 92-93 26.678073390871155 32.0 25.0 34.0 2.0 35.0 94-95 26.083956913860295 32.0 25.0 34.0 2.0 35.0 96-97 24.988356595884472 31.0 19.5 34.0 2.0 35.0 98-99 23.79052822936397 31.0 4.5 34.0 2.0 35.0 100-101 18.264524626839293 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2211183356771187 1101 2 0.20548047176343687 1101 3 0.23822806925473117 1101 4 0.24904128047037233 1101 5 0.2729879723690729 1101 6 0.2596869766529437 1101 7 0.28777289437552867 1101 8 0.2539896614543409 1101 9 0.3587462206256191 1101 10-11 0.38547848290658493 1101 12-13 0.23705883743278378 1101 14-15 0.44351954185644615 1101 16-17 0.32638477208794114 1101 18-19 0.31473525297262483 1101 20-21 0.3390622798986769 1101 22-23 0.4096776854078925 1101 24-25 0.44965166310640825 1101 26-27 0.38753217627389347 1101 28-29 0.44471235424810374 1101 30-31 0.3776120012932651 1101 32-33 0.4550796503131238 1101 34-35 0.6609336408373849 1101 36-37 0.7502257917615722 1101 38-39 0.7787690042021325 1101 40-41 0.7688430990288353 1101 42-43 0.6738273504941006 1101 44-45 0.7326167015380847 1101 46-47 0.858050882802182 1101 48-49 0.8048487760269225 1101 50-51 0.6598513645536102 1101 52-53 0.9199765091907253 1101 54-55 0.8581960928665566 1101 56-57 0.7583233141444623 1101 58-59 0.7691527065610941 1101 60-61 0.9414610181037801 1101 62-63 1.1306924270615468 1101 64-65 0.9408264499227954 1101 66-67 0.6552488199268041 1101 68-69 0.7531464274293143 1101 70-71 0.8682315348057585 1101 72-73 0.8619159842649431 1101 74-75 1.054678398642782 1101 76-77 0.8599839273130208 1101 78-79 1.0356932913296397 1101 80-81 1.0606354742504323 1101 82-83 1.0079562128724753 1101 84-85 1.1384962726480445 1101 86-87 1.2101699564904784 1101 88-89 1.473449808490006 1101 90-91 1.425505382590952 1101 92-93 1.2573797151193276 1101 94-95 1.3034908731906754 1101 96-97 1.4447041722453662 1101 98-99 1.628271552805952 1101 100-101 1.2568085361527928 1102 1 0.15997858624356098 1102 2 0.08446946184653825 1102 3 0.15173094802158005 1102 4 0.08047581917346491 1102 5 0.13280960403462672 1102 6 0.05180958346568332 1102 7 0.143435158454011 1102 8 0.14865840386600127 1102 9 0.07218529091243653 1102 10-11 0.16478149342612625 1102 12-13 0.1682446412276164 1102 14-15 0.28699290920127396 1102 16-17 0.19121625566464928 1102 18-19 0.20241608059224347 1102 20-21 0.2773715915056201 1102 22-23 0.3181615545650587 1102 24-25 0.32237826444816164 1102 26-27 0.31583153257630414 1102 28-29 0.3181789567124085 1102 30-31 0.31439168183728583 1102 32-33 0.257451198820867 1102 34-35 0.3595298542767438 1102 36-37 0.3678381138880553 1102 38-39 0.4768786779812757 1102 40-41 0.4885277197081095 1102 42-43 0.5735413939724836 1102 44-45 0.4601601045979322 1102 46-47 0.5796512349304592 1102 48-49 0.6625709314537502 1102 50-51 0.515032997164468 1102 52-53 0.6724309200996146 1102 54-55 0.7239341885969992 1102 56-57 0.7139530831636662 1102 58-59 0.6498278948108833 1102 60-61 0.5844271671143062 1102 62-63 0.6363365234478096 1102 64-65 0.6100568782963052 1102 66-67 0.5185902573309846 1102 68-69 0.4074917553783095 1102 70-71 0.672979358375315 1102 72-73 0.6445018820000463 1102 74-75 0.6915868777853795 1102 76-77 0.5170874949751187 1102 78-79 0.8539933270742068 1102 80-81 0.8901359661074686 1102 82-83 1.0295488843503797 1102 84-85 1.0113197683483577 1102 86-87 1.1822764930193586 1102 88-89 0.9971381158814481 1102 90-91 0.8791172084292462 1102 92-93 1.2007544156478147 1102 94-95 1.4351576038627627 1102 96-97 1.6369561684759368 1102 98-99 2.157597457571793 1102 100-101 2.078905471868705 1103 1 0.10931194577404568 1103 2 0.04198095584700212 1103 3 0.16115729973683557 1103 4 0.12082270799197659 1103 5 0.05507629720664653 1103 6 0.08665218095953975 1103 7 0.11859910254312211 1103 8 0.16861890010170555 1103 9 0.2718555535234728 1103 10-11 0.18614074421839888 1103 12-13 0.14184455755083292 1103 14-15 0.1225018582254691 1103 16-17 0.07520197513475324 1103 18-19 0.20013721730207124 1103 20-21 0.23497352346853262 1103 22-23 0.2473076006057866 1103 24-25 0.20968794021217008 1103 26-27 0.3027090709069782 1103 28-29 0.1932020718465779 1103 30-31 0.31802364729403365 1103 32-33 0.32758020438467383 1103 34-35 0.414608884920483 1103 36-37 0.42685971960396785 1103 38-39 0.31366647180352913 1103 40-41 0.3143621182638725 1103 42-43 0.417430157012312 1103 44-45 0.30805587614643315 1103 46-47 0.3526580169108229 1103 48-49 0.4303990565621021 1103 50-51 0.5790639413945868 1103 52-53 0.5734432474006681 1103 54-55 0.5580998593866298 1103 56-57 0.7106606212400735 1103 58-59 0.5807231786300591 1103 60-61 0.39906083846795326 1103 62-63 0.5221153994512946 1103 64-65 0.6884246955573019 1103 66-67 0.5902142341973544 1103 68-69 0.5366088497134811 1103 70-71 0.5427252883780653 1103 72-73 0.4866549874027406 1103 74-75 0.5449693153687427 1103 76-77 0.34013854243742614 1103 78-79 0.6979557696042029 1103 80-81 0.5362181242288528 1103 82-83 0.7002665745937122 1103 84-85 0.9405562523515307 1103 86-87 0.9321773868815946 1103 88-89 0.8734912180480272 1103 90-91 0.9167408455031527 1103 92-93 1.025489689573714 1103 94-95 1.281150999868931 1103 96-97 0.8893118895872227 1103 98-99 1.2504731998565397 1103 100-101 1.4985280826306457 1104 1 0.14394885686951397 1104 2 0.16106829019562952 1104 3 0.11670390707532619 1104 4 0.1379018674694521 1104 5 0.14874932035678512 1104 6 0.13401086974079846 1104 7 0.19425265357308064 1104 8 0.18573067807994192 1104 9 0.15983293652990938 1104 10-11 0.2776572907964905 1104 12-13 0.17804125160954243 1104 14-15 0.39860425107369934 1104 16-17 0.3370616121020902 1104 18-19 0.3413885417256921 1104 20-21 0.4239327795867496 1104 22-23 0.29058138455433635 1104 24-25 0.23678097942411114 1104 26-27 0.2610608693111516 1104 28-29 0.26376179407193234 1104 30-31 0.1813976825175132 1104 32-33 0.33132570809306117 1104 34-35 0.336853971337014 1104 36-37 0.48245008072450446 1104 38-39 0.5540611669105289 1104 40-41 0.4456943253216181 1104 42-43 0.3731609464025283 1104 44-45 0.380864587760648 1104 46-47 0.45375203017868415 1104 48-49 0.3172623490510702 1104 50-51 0.4360163498771996 1104 52-53 0.31151162477647887 1104 54-55 0.6378526561418809 1104 56-57 0.5327331670353246 1104 58-59 0.5581846381842368 1104 60-61 0.6815407106034286 1104 62-63 0.631070190209229 1104 64-65 0.699751751141477 1104 66-67 0.5208847355999566 1104 68-69 0.44370292371924336 1104 70-71 0.5910324444272206 1104 72-73 0.5740225169224864 1104 74-75 0.6230012050147273 1104 76-77 0.6301532882493213 1104 78-79 1.0148984937664132 1104 80-81 1.018661216192779 1104 82-83 0.8738433956621137 1104 84-85 1.0883259342424125 1104 86-87 1.109405230483759 1104 88-89 1.138261775014552 1104 90-91 1.0431395043203828 1104 92-93 1.1478956751810792 1104 94-95 1.2817884666036825 1104 96-97 1.3234036780483684 1104 98-99 1.6992438760493123 1104 100-101 1.5840034579121216 1105 1 0.027034389980265416 1105 2 0.05688956833382264 1105 3 0.05307595023165135 1105 4 0.1941940788489731 1105 5 0.0924707563679874 1105 6 -0.004792920085016306 1105 7 0.005159866601204044 1105 8 0.1047295928394334 1105 9 0.14186423600170173 1105 10-11 0.11194985373279565 1105 12-13 0.062182819814829315 1105 14-15 0.10667073242699132 1105 16-17 0.044896360760823484 1105 18-19 0.12175016482623846 1105 20-21 0.11160924034886222 1105 22-23 0.12667431127548667 1105 24-25 0.047623503820183544 1105 26-27 -0.03647681386413382 1105 28-29 0.2493823573052012 1105 30-31 0.16060943106488423 1105 32-33 0.1926330080277765 1105 34-35 0.2813516709328354 1105 36-37 0.22805643975351586 1105 38-39 0.21386337800618094 1105 40-41 0.3622154198205223 1105 42-43 0.41242776490096134 1105 44-45 0.36681432605828235 1105 46-47 0.21958464111013143 1105 48-49 0.3091533424282673 1105 50-51 0.3396745056670696 1105 52-53 0.2388056965682992 1105 54-55 0.373400069360855 1105 56-57 0.42835382852981496 1105 58-59 0.18080535208471105 1105 60-61 0.3718505718804366 1105 62-63 0.35222233568823214 1105 64-65 0.06357863235240302 1105 66-67 0.22515478580051962 1105 68-69 0.29315282469377735 1105 70-71 0.47481590509980975 1105 72-73 0.45059169390840026 1105 74-75 0.44527696746715506 1105 76-77 0.1896559118237171 1105 78-79 0.3126209626121508 1105 80-81 0.41219601048838683 1105 82-83 0.4174315239606905 1105 84-85 0.5683802726933358 1105 86-87 0.7136513704557679 1105 88-89 1.0339509464104957 1105 90-91 0.5736954662649048 1105 92-93 0.711886106722492 1105 94-95 1.0442411647474223 1105 96-97 1.2387450920927385 1105 98-99 1.076139225673625 1105 100-101 0.9570570670464846 1106 1 0.11106408047090355 1106 2 0.059735119216348664 1106 3 0.05434594441747009 1106 4 -0.031193285292417272 1106 5 0.025849641149704894 1106 6 -0.007530250911607084 1106 7 0.12499554187313322 1106 8 0.03322796529467098 1106 9 -0.07115951723959313 1106 10-11 0.09801864349305589 1106 12-13 0.17541548020331987 1106 14-15 0.09975525865656465 1106 16-17 -0.013483585001829113 1106 18-19 0.011418756819651321 1106 20-21 0.09127607111391711 1106 22-23 0.05842299884206881 1106 24-25 0.09354753334091725 1106 26-27 0.06197691251313131 1106 28-29 -0.11281213323297123 1106 30-31 0.13797021975567958 1106 32-33 0.07633305174019256 1106 34-35 -0.04992159107428762 1106 36-37 0.06975543097283321 1106 38-39 0.04513133510860712 1106 40-41 0.04076896328466262 1106 42-43 0.05269156720201096 1106 44-45 0.041693692525342385 1106 46-47 -0.0847280342169654 1106 48-49 0.16713945206578984 1106 50-51 0.07635813388237267 1106 52-53 0.09187946445113937 1106 54-55 0.20408448694597325 1106 56-57 0.35107569025429086 1106 58-59 0.32816711687796385 1106 60-61 0.3778477666468234 1106 62-63 0.3577054851889301 1106 64-65 0.06634036574062208 1106 66-67 0.2888831364844222 1106 68-69 0.07290962664258416 1106 70-71 0.27784744594121236 1106 72-73 0.2710790236740088 1106 74-75 0.30933507102738034 1106 76-77 -0.03026633949296098 1106 78-79 0.2809947122461125 1106 80-81 0.2657832931464412 1106 82-83 0.2719625491653517 1106 84-85 0.19875838508092514 1106 86-87 0.5768946406536593 1106 88-89 0.8218322099114808 1106 90-91 0.869200938804596 1106 92-93 0.7718028833775215 1106 94-95 1.2255357524714476 1106 96-97 1.3202337332307863 1106 98-99 1.4284777337646624 1106 100-101 1.3647029200963807 1107 1 -0.008649813357088476 1107 2 0.03577395710279063 1107 3 -0.019847205334301066 1107 4 0.06964601667143455 1107 5 -0.01717913798152182 1107 6 0.039334009510987755 1107 7 -0.00846501103669084 1107 8 0.02536755209828101 1107 9 0.05039015814111991 1107 10-11 0.10818974139765913 1107 12-13 0.1657992019529715 1107 14-15 0.12019575431247631 1107 16-17 0.11724806203380211 1107 18-19 -0.020170728722348485 1107 20-21 -0.009080923290397891 1107 22-23 0.015734311648010646 1107 24-25 0.06272785049451812 1107 26-27 0.1115409718423237 1107 28-29 0.0028012527039749102 1107 30-31 0.01830049702763148 1107 32-33 0.014086524916336884 1107 34-35 0.05560316669956933 1107 36-37 0.1966357666119194 1107 38-39 0.07801505941149145 1107 40-41 0.15196421792978043 1107 42-43 0.10454971516141853 1107 44-45 0.07787110000348463 1107 46-47 -0.005212905232248488 1107 48-49 -0.053524043813787614 1107 50-51 -0.007924920653870515 1107 52-53 -0.2501090214452617 1107 54-55 -0.05115451189689679 1107 56-57 0.05707591289705505 1107 58-59 0.009518589128809651 1107 60-61 0.19349138297818058 1107 62-63 0.17174004792568098 1107 64-65 0.07817464598659285 1107 66-67 0.14679466560109233 1107 68-69 0.09530003380310603 1107 70-71 0.3223752339035926 1107 72-73 0.38011474558297564 1107 74-75 0.29271677980042554 1107 76-77 -0.027407728885972205 1107 78-79 0.23971669168331644 1107 80-81 0.363750707640893 1107 82-83 0.38345832048467443 1107 84-85 0.4573740474891217 1107 86-87 0.305634298743815 1107 88-89 0.49927875266757127 1107 90-91 0.41334471905414105 1107 92-93 0.6260499984685133 1107 94-95 0.49578603154088086 1107 96-97 0.6357974330633986 1107 98-99 0.8257623090123047 1107 100-101 0.8849927744287704 1108 1 0.012822627529999409 1108 2 -0.0396427877728982 1108 3 0.0770320186084632 1108 4 0.05562449284852988 1108 5 -0.06572361121620673 1108 6 0.08494184306680097 1108 7 0.05671045855825696 1108 8 0.021382824092668784 1108 9 0.16221997669967436 1108 10-11 0.10953747078429643 1108 12-13 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-0.9252316410874357 2316 62-63 -1.1092069150305264 2316 64-65 -1.0981393892161506 2316 66-67 -1.1965198228401093 2316 68-69 -1.1283474892059786 2316 70-71 -1.083356868369485 2316 72-73 -1.1274656981346247 2316 74-75 -1.25228210237427 2316 76-77 -1.1661532642021264 2316 78-79 -1.3731859035853269 2316 80-81 -1.5097709225841207 2316 82-83 -1.4960315602439778 2316 84-85 -1.9629282662260046 2316 86-87 -1.8293116485998944 2316 88-89 -2.078158646061322 2316 90-91 -2.2510503257016303 2316 92-93 -2.29590258374866 2316 94-95 -2.115737505299098 2316 96-97 -1.9701962281137746 2316 98-99 -2.3948057987191795 2316 100-101 -1.49720452397683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 46.0 6 121.0 7 179.0 8 337.0 9 666.0 10 1165.0 11 1645.0 12 2742.0 13 3732.0 14 4451.0 15 5860.0 16 7587.0 17 10411.0 18 10688.0 19 8930.0 20 8802.0 21 9563.0 22 10886.0 23 12250.0 24 14262.0 25 16865.0 26 20009.0 27 24063.0 28 29596.0 29 35623.0 30 43831.0 31 53378.0 32 65684.0 33 83787.0 34 113878.0 35 161994.0 36 241199.0 37 297578.0 38 136286.0 39 4773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.37170761300682 18.15991579058683 14.148767644216619 16.31960895218973 2 13.263362019077164 27.919704867428063 32.433395223570685 26.383537889924092 3 32.354732939814 23.812216841768215 30.905752881222725 12.927297337195062 4 11.406516286558038 15.174058495630604 44.22123299491225 29.19819222289911 5 9.709975278558048 43.938811139133826 34.67509704569412 11.676116536614 6 24.301932874389152 38.652428446776824 15.5133112524491 21.532327426384924 7 22.805270303705722 36.82455261259276 21.560604264951206 18.80957281875032 8 21.293152500178465 23.957274251374162 20.413313123710473 34.336260124736896 9 18.240917278465176 27.002578875399635 28.031287490201866 26.725216355933316 10-11 25.139322340019284 22.110166008606416 26.96550017776983 25.785011473604474 12-13 35.617138536410245 21.826254074325117 23.511532861082408 19.045074528182234 14-15 25.573797419094156 25.93374087882758 32.966863508760866 15.525598193317395 16-17 17.64191171247035 28.67093948230663 32.82724898940806 20.859899815814963 18-19 16.564105087557948 37.081572667864734 27.87681244295236 18.477509801624954 20-21 21.89122842764837 27.541467498615614 23.015960473415266 27.55134360032075 22-23 18.233848068823608 27.674119138360375 25.98634533643547 28.10568745638055 24-25 26.64380029884758 27.395146914911606 24.943654201747066 21.017398584493748 26-27 25.4359953883787 20.935991403283648 34.76763257285014 18.860380635487513 28-29 17.855991882883988 33.45396038608974 29.371353206129143 19.318694524897133 30-31 19.883954072315245 28.486177269441537 26.759522814487998 24.87034584375522 32-33 21.080279192197256 28.789529530237157 28.392371629736978 21.73781964782861 34-35 26.11861983110752 24.369321816907686 27.01489506083415 22.497163291150645 36-37 23.822970557020167 23.065389022641142 33.94476174499203 19.16687867534666 38-39 23.028273203548974 26.31985341643063 32.21216911174779 18.439704268272607 40-41 20.194469290063722 29.383555638573235 30.415508471903735 20.006466599459312 42-43 17.699499311067555 31.554977363648206 24.80676623149794 25.9387570937863 44-45 21.74298904459164 25.855754829507454 25.33861824764754 27.06263787825337 46-47 25.450043039459075 29.324460689934313 25.332810760835063 19.892685509771553 48-49 23.915225174898843 26.535034522335906 27.92147260183777 21.62826770092748 50-51 17.535618271651828 29.085696622493103 34.31177743786858 19.06690766798649 52-53 20.67106205172028 32.31744632425356 26.143739209925727 20.86775241410044 54-55 16.246741752762016 26.075264904770123 30.384953117971943 27.29304022449592 56-57 23.616392804099032 25.89912376453433 26.320746674872492 24.163736756494146 58-59 28.228000224552925 24.785998986739596 25.28070847832822 21.705292310379264 60-61 21.423299158129797 26.29789189380251 33.08299702188502 19.195811926182667 62-63 21.37197268765455 26.291251289102785 30.871793876623162 21.4649821466195 64-65 19.472863843778242 33.04218946656391 27.925380768356444 19.559565921301406 66-67 20.786794668714478 26.35202827198968 25.839116770622418 27.022060288673426 68-69 17.665091070388954 29.068365168695077 28.1333689113138 25.133174849602174 70-71 24.68381736066808 28.164260766600606 24.027836062199366 23.12408581053195 72-73 21.915728090474794 22.85666068556086 31.561699299385882 23.665911924578463 74-75 18.050256536073412 33.68350178151013 26.461853643796385 21.80438803862006 76-77 21.6061763741895 24.956672083017573 26.622780929126733 26.8143706136662 78-79 20.91406281946698 25.95868683347276 30.046512659469254 23.08073768759101 80-81 23.87323763075475 26.630271687113144 27.262655826257753 22.233834855874356 82-83 20.589974058772857 25.199549233926383 31.417993356329056 22.792483350971704 84-85 19.692918971235585 27.042092653113414 29.296235679214405 23.968752696436596 86-87 20.295140237743762 28.63653201549441 27.824872082311288 23.243455664450536 88-89 20.20786392892965 27.022623987147977 25.415947116264725 27.353564967657647 90-91 21.16448717962097 22.956080840167818 28.298267785229996 27.581164194981213 92-93 23.01433178295863 28.49161778862647 28.098895470646497 20.3951549577684 94-95 21.097452747772927 27.09761839003346 27.675564335904486 24.129364526289123 96-97 14.80645959426895 27.383345912368135 31.710464578754806 26.099729914608105 98-99 20.013175066204532 28.984521896183807 24.844945203229383 26.157357834382278 100-101 19.454191614068915 24.768118242196625 27.648248039149774 28.129442104584683 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6319.0 1 3903.5 2 1339.5 3 1108.0 4 837.5 5 811.0 6 1473.5 7 2534.0 8 3326.5 9 3437.0 10 2583.5 11 1659.0 12 1437.0 13 1375.0 14 1315.5 15 1221.0 16 1207.5 17 1270.0 18 1350.0 19 1521.5 20 1751.5 21 2067.5 22 2448.0 23 2809.0 24 3193.5 25 4019.5 26 5487.5 27 6758.5 28 7361.5 29 8095.5 30 9392.0 31 10713.5 32 12767.5 33 15796.5 34 18989.0 35 21112.0 36 22897.5 37 25050.5 38 24032.0 39 21438.5 40 19932.0 41 18584.0 42 17795.5 43 17808.5 44 19532.0 45 22948.0 46 41418.5 47 131491.0 48 323075.0 49 308378.5 50 126996.0 51 60657.5 52 31005.0 53 13377.5 54 6348.5 55 4468.5 56 3581.0 57 2845.0 58 2261.0 59 1823.5 60 1462.0 61 1192.0 62 886.5 63 559.0 64 292.5 65 182.5 66 153.5 67 124.0 68 96.5 69 68.5 70 53.5 71 63.5 72 61.5 73 61.5 74 56.0 75 44.5 76 41.5 77 28.5 78 18.0 79 15.0 80 10.0 81 8.5 82 6.0 83 4.0 84 3.5 85 1.5 86 1.5 87 1.0 88 1.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3817373206447951 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.9701873409861E-4 16-17 0.0023910562022958297 18-19 2.7722390751255994E-4 20-21 0.0 22-23 0.0 24-25 6.930597687813999E-5 26-27 3.465298843906999E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01275229974557776 36-37 0.02259374846227364 38-39 0.010430549520160069 40-41 0.0054405191849339896 42-43 0.0045048884970790995 44-45 0.0019405673525879198 46-47 0.0010395896531721 48-49 0.0029801570057600194 50-51 5.544478150251199E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.930597687814E-4 60-61 0.00124750758380652 62-63 9.009776994158198E-4 64-65 3.465298843907E-4 66-67 0.0024603621791739697 68-69 0.0025643211444911795 70-71 3.465298843906999E-5 72-73 0.0 74-75 0.0 76-77 0.08694434799362662 78-79 0.00304946298263816 80-81 2.7722390751255994E-4 82-83 0.0 84-85 3.465298843906999E-5 86-87 0.09183041936353549 88-89 0.4308059522745182 90-91 0.3810096078875746 92-93 0.0 94-95 5.8910080346419E-4 96-97 0.0 98-99 0.0 100-101 5.544478150251199E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1442877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.944515052604501 #Duplication Level Percentage of deduplicated Percentage of total 1 72.42390926590868 11.547641114587433 2 13.002121372607045 4.146250398826474 3 4.99888319857471 2.3911430521765857 4 2.46811130182965 1.5741135121410461 5 1.460611382998033 1.1644370091108807 6 0.9671507132646185 0.9252449463470933 7 0.655445937016003 0.7315537326244085 8 0.48995091569032123 0.6249623800209348 9 0.38691061089153866 0.5552191853435287 >10 2.556140196869395 7.975741932663545 >50 0.3140611547611443 3.4266760061250885 >100 0.2145867364158871 6.374067565581493 >500 0.026497552472407114 2.8329035267566467 >1k 0.019547374774726557 7.743560534985414 >5k 0.00564701937936545 6.43148473402512 >10k+ 0.010425266546520831 41.55500036868432 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 101023 7.001497702160337 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 54556 3.7810568745638053 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 39277 2.7221308538427045 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 32107 2.2252069996264408 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 31594 2.189653033487955 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 27187 1.8842215933859918 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 26103 1.8090939144500884 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 25387 1.75947083500534 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 25320 1.7548273345545047 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 24144 1.673323505745812 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 23873 1.654541586011836 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 23299 1.6147599552837837 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 20404 1.4141191522215686 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 17563 1.2172208719107727 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 16932 1.1734888005006665 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 14021 0.9717391018084008 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 13830 0.9585016602246761 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 13466 0.9332742846410332 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 13335 0.9241952016699969 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 12655 0.8770671373928616 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12246 0.8487209928497023 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11132 0.7715141346074544 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10505 0.7280592871048607 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 10020 0.6944458883189627 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9603 0.6655452959607784 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9395 0.6511296527701252 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8809 0.6105163503195352 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8529 0.591110676793656 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 8229 0.570318883730214 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7544 0.5228442895686881 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7241 0.5018445785746117 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6989 0.4843794724013204 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5756 0.3989252029105738 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5359 0.37141073008995223 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5231 0.3625395650495503 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5171 0.3583812064368619 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5003 0.3467378023213344 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4955 0.3434111154311837 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4880 0.3382131671653232 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4776 0.3310053455699966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4606 0.3192233295007128 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4578 0.3172827621481249 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4554 0.31561941870304955 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 4538 0.3145105230729993 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3993 0.276738765674413 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3907 0.270778451662893 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3331 0.23085820898108433 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3331 0.23085820898108433 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3319 0.23002653725854666 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3285 0.2276701340446899 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3176 0.2201157825649726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3126 0.21665048372106563 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2928 0.2029279002991939 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2871 0.19897745961713992 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2692 0.18657168975595287 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2542 0.17617579322423185 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2525 0.17499759161730347 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2391 0.16571059071563274 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2281 0.15808693325903733 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2170 0.1503939698255638 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 2148 0.1488692383342447 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1902 0.13181996802222226 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1883 0.13050315446153762 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1730 0.1198993399991822 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1692 0.11726571287781287 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1625 0.1126222124269775 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1541 0.10680051036921373 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1473 0.10208770394150021 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0395896531721E-4 0.0 12-13 0.0 0.0 0.0 2.0791793063441999E-4 0.0 14-15 0.0 0.0 0.0 4.8514183814698E-4 0.0 16-17 0.0 0.0 0.0 9.3563068785489E-4 0.0 18-19 0.0 0.0 0.0 0.00152473149131908 0.0 20-21 0.0 0.0 0.0 0.00235640321385676 0.0 22-23 0.0 0.0 0.0 0.00447023550864003 0.0 24-25 0.0 0.0 0.0 0.00731178056064377 0.0 26-27 0.0 0.0 0.0 0.01015332561264751 0.0 28-29 0.0 0.0 0.0 0.026232312248375987 0.0 30-31 0.0 0.0 0.0 0.06130113654871482 0.0 32-33 0.0 0.0 0.0 0.14429504386028746 0.0 34-35 0.0 0.0 0.0 0.2783328031426102 0.0 36-37 0.0 0.0 0.0 0.4374939790432587 0.0 38-39 0.0 0.0 0.0 0.6385159649783038 0.0 40-41 0.0 0.0 0.0 0.8505922542254121 0.0 42-43 0.0 0.0 0.0 1.073410969888632 0.0 44-45 0.0 0.0 0.0 1.3451250522393803 0.0 46-47 0.0 0.0 0.0 1.707491352346735 0.0 48-49 0.0 0.0 0.0 2.0319472831017475 0.0 50-51 0.0 0.0 0.0 2.374942562671662 0.0 52-53 0.0 0.0 0.0 2.6751414015193253 0.0 54-55 0.0 0.0 0.0 3.0266959692336908 0.0 56-57 0.0 0.0 0.0 3.5497828297214524 0.0 58-59 0.0 0.0 0.0 4.0308702682210615 0.0 60-61 0.0 0.0 0.0 4.658193317933545 0.0 62-63 0.0 0.0 0.0 5.204393721710167 0.0 64-65 0.0 0.0 0.0 5.660461702556766 0.0 66-67 0.0 0.0 0.0 6.232582541685812 0.0 68-69 0.0 0.0 0.0 6.932850132062539 0.0 70-71 0.0 0.0 0.0 7.631315767040434 0.0 72-73 0.0 0.0 0.0 8.295336331509894 0.0 74-75 0.0 0.0 0.0 9.111622127180626 0.0 76-77 0.0 0.0 0.0 9.735098695176372 0.0 78-79 0.0 0.0 0.0 10.782450617758826 0.0 80-81 0.0 0.0 0.0 11.835173753549332 0.0 82-83 0.0 0.0 0.0 12.634652849827116 0.0 84-85 0.0 0.0 0.0 13.73301397139188 0.0 86-87 0.0 0.0 0.0 14.852478762916036 0.0 88-89 0.0 0.0 0.0 15.874534003937965 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCG 30 1.5029291E-6 47.454674 94-95 GTAAGCG 900 0.0 43.763752 94-95 CTCCGAC 335 0.0 38.24705 94-95 TTACTCG 80 5.4569682E-11 32.625084 94-95 AGGCTTA 265 0.0 32.23336 6 AACTTCG 30 0.0038342597 31.8628 88-89 GCAAGCG 45 2.4300223E-5 31.636448 94-95 GCTTATG 450 0.0 28.583742 1 CTTTGCG 1450 0.0 28.472805 2 TTTGCGT 1460 0.0 28.277784 3 CTTATGT 440 0.0 28.041397 2 GTACCAG 85 6.2989665E-4 28.023275 1 GCTTTGC 1495 0.0 27.723364 1 AAGCAGG 1460 0.0 27.302689 94-95 GTTGATC 1565 0.0 26.83539 94-95 CCACTGT 845 0.0 26.394909 94-95 AGACGGC 210 0.0 25.987083 94-95 TTGCGTT 1590 0.0 25.965765 4 ATGCCGC 55 9.543231E-5 25.884365 94-95 ACGAGAG 55 9.543231E-5 25.884365 94-95 >>END_MODULE