##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939044_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1453372 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.989582845960978 33.0 31.0 34.0 30.0 34.0 2 32.18602601398678 33.0 31.0 34.0 30.0 34.0 3 32.30951332487484 34.0 31.0 34.0 30.0 34.0 4 35.773025075479644 37.0 35.0 37.0 35.0 37.0 5 35.721007422738296 37.0 35.0 37.0 33.0 37.0 6 35.85645863550419 37.0 35.0 37.0 35.0 37.0 7 35.83113752019442 37.0 35.0 37.0 35.0 37.0 8 35.85963538584753 37.0 35.0 37.0 35.0 37.0 9 37.41827556881514 39.0 37.0 39.0 34.0 39.0 10-11 37.31406652942262 39.0 37.0 39.0 33.5 39.0 12-13 37.30899074703517 39.0 37.0 39.0 34.0 39.0 14-15 38.17555691178858 40.0 37.5 41.0 33.0 41.0 16-17 38.31377100976212 40.0 37.5 41.0 33.5 41.0 18-19 38.62812996259733 40.0 38.0 41.0 34.0 41.0 20-21 38.604779781088396 40.0 38.0 41.0 34.0 41.0 22-23 38.59018785280024 40.0 38.0 41.0 34.0 41.0 24-25 38.42017872918977 40.0 38.0 41.0 33.5 41.0 26-27 38.072944160201246 40.0 37.5 41.0 33.5 41.0 28-29 37.96956801149327 40.0 37.0 41.0 33.0 41.0 30-31 38.04592045257512 40.0 37.0 41.0 33.0 41.0 32-33 37.91030101034009 40.0 37.0 41.0 33.0 41.0 34-35 37.785132436843426 40.0 37.0 41.0 33.0 41.0 36-37 37.5988143434716 40.0 36.5 41.0 32.5 41.0 38-39 37.38924342838585 39.0 36.5 41.0 32.0 41.0 40-41 37.47666736389583 40.0 37.0 41.0 32.5 41.0 42-43 37.38097128608504 40.0 37.0 41.0 32.0 41.0 44-45 37.23260734347434 40.0 37.0 41.0 31.0 41.0 46-47 37.012089471931475 40.0 36.0 41.0 31.0 41.0 48-49 36.71564540943406 39.0 36.0 41.0 30.5 41.0 50-51 36.46258769262102 39.0 36.0 40.0 30.0 41.0 52-53 36.32335114478606 39.0 35.5 40.0 29.0 41.0 54-55 36.2486393710626 39.0 35.5 40.0 29.0 41.0 56-57 35.93482707799517 38.5 35.0 40.0 28.5 41.0 58-59 35.725089309550484 39.0 35.0 40.0 28.0 41.0 60-61 34.974872916225166 38.0 34.0 40.0 27.0 41.0 62-63 34.770013114329984 38.0 34.0 40.0 26.5 41.0 64-65 34.45824296876505 37.0 33.5 39.5 26.0 40.5 66-67 33.8289832197125 36.5 33.0 39.0 26.0 40.0 68-69 33.32772304681802 36.0 32.5 38.5 26.0 39.5 70-71 32.571975378636715 35.0 31.5 37.5 25.0 39.0 72-73 31.901339436840672 35.0 31.0 37.0 23.0 38.5 74-75 31.506395472047075 34.0 31.0 36.5 22.0 37.0 76-77 29.462204101909215 32.5 28.5 34.0 20.0 36.0 78-79 30.484017856405657 34.0 30.0 35.0 20.0 36.0 80-81 30.43583920703027 34.0 30.5 35.0 20.0 36.0 82-83 30.107267788288205 34.0 30.0 35.0 19.0 36.0 84-85 29.723539809491307 34.0 29.0 35.0 17.0 35.0 86-87 29.379309289018916 34.0 29.0 35.0 9.0 35.0 88-89 28.703951913205977 33.5 29.0 35.0 4.0 35.0 90-91 28.10318074106285 33.0 27.5 35.0 2.0 35.0 92-93 27.86732371340579 33.0 27.0 35.0 2.0 35.0 94-95 27.455448088995798 33.0 27.0 34.0 2.0 35.0 96-97 26.666653478944138 32.0 25.0 34.0 2.0 35.0 98-99 25.828028887304832 32.0 24.5 34.0 2.0 35.0 100-101 21.433094555282473 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1811127513834876 1101 2 0.11901906842912524 1101 3 0.23419649293055045 1101 4 0.11859368999964914 1101 5 0.22584225005165592 1101 6 0.12966428530562268 1101 7 0.17838715439339836 1101 8 0.19020092501823171 1101 9 0.2099456646151765 1101 10-11 0.250295360064122 1101 12-13 0.19155150972374457 1101 14-15 0.2950800508684992 1101 16-17 0.28268161981687 1101 18-19 0.3270782402666228 1101 20-21 0.37316602896770235 1101 22-23 0.2713419264562802 1101 24-25 0.24841922934999872 1101 26-27 0.1978084911300968 1101 28-29 0.2785883141892924 1101 30-31 0.14863442967567408 1101 32-33 0.31462276441477854 1101 34-35 0.43903709960354576 1101 36-37 0.494689009508555 1101 38-39 0.3578950203332809 1101 40-41 0.3591657436284734 1101 42-43 0.34006584462807155 1101 44-45 0.4172389594737922 1101 46-47 0.40370303299385313 1101 48-49 0.2656406046750348 1101 50-51 0.36981537300822964 1101 52-53 0.47512667687193755 1101 54-55 0.3638686133306308 1101 56-57 0.1391372185944988 1101 58-59 0.4171659329540489 1101 60-61 0.7363709834592314 1101 62-63 0.5645826647886452 1101 64-65 0.41551036830363586 1101 66-67 0.55075925789302 1101 68-69 0.5053310936114102 1101 70-71 0.7126232309201797 1101 72-73 0.4573421317542632 1101 74-75 0.47545065515869567 1101 76-77 0.3892542408206694 1101 78-79 0.5780145437972379 1101 80-81 0.6538228582215879 1101 82-83 0.8343498701295431 1101 84-85 0.8290359559477096 1101 86-87 0.8294117313656635 1101 88-89 0.8674106285686953 1101 90-91 0.9250022153862894 1101 92-93 1.0250796739143766 1101 94-95 0.8887754906652638 1101 96-97 0.6639112274536814 1101 98-99 0.6798646628674625 1101 100-101 1.2194802369393685 1102 1 -0.04513333298538669 1102 2 -0.021513528634969248 1102 3 0.026372846874565425 1102 4 -0.033676622653601385 1102 5 -0.008434268435159709 1102 6 0.0994652285090396 1102 7 0.07673224717679972 1102 8 0.07021453555204005 1102 9 0.05318453331899775 1102 10-11 0.11906712993214086 1102 12-13 0.1847117233499418 1102 14-15 0.15542660442820022 1102 16-17 0.25750624173278425 1102 18-19 0.18156990819809238 1102 20-21 0.165188689081873 1102 22-23 0.15893995764106705 1102 24-25 0.22810589119124103 1102 26-27 0.11555845729554193 1102 28-29 0.25371758412442347 1102 30-31 0.27575566479326596 1102 32-33 0.21707348239235813 1102 34-35 0.20193820351428116 1102 36-37 0.1484516043206625 1102 38-39 0.09611799090351525 1102 40-41 0.2431808730189502 1102 42-43 0.07063919110436956 1102 44-45 0.07314587087436308 1102 46-47 0.04905246591567192 1102 48-49 -0.05010295840272505 1102 50-51 0.09254019749315745 1102 52-53 0.1542991629915491 1102 54-55 -0.024079269940962433 1102 56-57 0.03166523278802913 1102 58-59 -0.025920776118603328 1102 60-61 -0.15863120003421471 1102 62-63 0.1341204929222286 1102 64-65 -0.013671295611516143 1102 66-67 -0.055022643713336095 1102 68-69 0.048691931108542974 1102 70-71 0.20339228635242534 1102 72-73 0.19521771591410086 1102 74-75 0.2468961497377684 1102 76-77 -0.002934605883844199 1102 78-79 0.004824227001648751 1102 80-81 0.2138130393501747 1102 82-83 0.11011605167894345 1102 84-85 0.026795532771885178 1102 86-87 0.1997503016130544 1102 88-89 0.2516394499150927 1102 90-91 0.33003833206647926 1102 92-93 0.5500484831077372 1102 94-95 0.4316774383852504 1102 96-97 0.0527281246793585 1102 98-99 0.6082904599795533 1102 100-101 1.1069407381730265 1103 1 -0.0026008853034511503 1103 2 0.06799818397377067 1103 3 0.05059287971528903 1103 4 0.021504088662474885 1103 5 0.10392401860672607 1103 6 0.07829999425686651 1103 7 0.14333078681276135 1103 8 0.038176270869790585 1103 9 -0.0015600121514225407 1103 10-11 0.16295283810388383 1103 12-13 0.04343602286432002 1103 14-15 0.15633878606534068 1103 16-17 0.09325732041077117 1103 18-19 0.14600683392216496 1103 20-21 0.1629767441424974 1103 22-23 0.13115035225378335 1103 24-25 0.15952304260793682 1103 26-27 0.006230676751918907 1103 28-29 0.14552798354097973 1103 30-31 0.27914755025251026 1103 32-33 0.21771681336744564 1103 34-35 0.15980753062883224 1103 36-37 0.1921528017842391 1103 38-39 0.1462751525392605 1103 40-41 0.22345651454849502 1103 42-43 0.2747402418328093 1103 44-45 0.3079106360981356 1103 46-47 0.38601814910751386 1103 48-49 0.2622368229793821 1103 50-51 0.127360315929387 1103 52-53 0.3065087606053609 1103 54-55 0.3253373377696249 1103 56-57 0.4255049770496342 1103 58-59 0.20889038972705265 1103 60-61 0.25219936017960265 1103 62-63 0.27305241611337294 1103 64-65 0.2897082527412067 1103 66-67 0.2377972148794072 1103 68-69 0.3171956802787932 1103 70-71 0.27022774210479383 1103 72-73 0.43693111073644886 1103 74-75 0.37975996336666995 1103 76-77 0.1969251550601534 1103 78-79 0.1963400643527322 1103 80-81 0.367684871006535 1103 82-83 0.5108239068129237 1103 84-85 0.4619176373730802 1103 86-87 0.5393837476180181 1103 88-89 0.7378849408664259 1103 90-91 0.6203866751821572 1103 92-93 0.5669838912585234 1103 94-95 0.634772414453451 1103 96-97 0.4459916823951815 1103 98-99 0.6857309143344423 1103 100-101 0.9323412028343121 1104 1 0.034679759240709984 1104 2 0.04857903026407939 1104 3 -0.04964538944189911 1104 4 0.003962174161095788 1104 5 -0.01604863261592726 1104 6 -0.16412800323870158 1104 7 -0.07632033148701112 1104 8 -0.05810207794234401 1104 9 -0.04687082731021519 1104 10-11 -0.12686322071555622 1104 12-13 -0.09946623025752643 1104 14-15 -0.04152298738764415 1104 16-17 -0.025950630300556554 1104 18-19 4.4579266855038213E-4 1104 20-21 0.10474645787166281 1104 22-23 -0.08001297081831638 1104 24-25 -0.045853122649980094 1104 26-27 -0.12533504075129542 1104 28-29 -0.019073122149656285 1104 30-31 -0.06322984765698436 1104 32-33 -0.12580790705760592 1104 34-35 0.0933780461250251 1104 36-37 -0.018935518679079166 1104 38-39 0.29543876475185726 1104 40-41 0.16058340374463143 1104 42-43 0.14518954006926776 1104 44-45 0.04242259738374088 1104 46-47 -0.027349489766884005 1104 48-49 -0.09809065580248699 1104 50-51 -0.08829827219542352 1104 52-53 -0.09553429958804571 1104 54-55 -0.06068668697906077 1104 56-57 -0.09404787696267647 1104 58-59 0.05045009615161433 1104 60-61 0.2357428095169709 1104 62-63 0.19540813935591927 1104 64-65 0.09887906085626952 1104 66-67 -0.014781950917665654 1104 68-69 -0.11126786410535772 1104 70-71 -0.00211542635323525 1104 72-73 -0.11540766626449184 1104 74-75 0.0611703493800988 1104 76-77 0.2833800643613458 1104 78-79 0.24395829672603142 1104 80-81 0.4248844149037154 1104 82-83 0.45092155151360913 1104 84-85 0.3966458018309744 1104 86-87 0.33687289941460463 1104 88-89 0.4795398136446103 1104 90-91 0.536821268559315 1104 92-93 0.668188226626711 1104 94-95 0.7238515998164203 1104 96-97 0.8919585529819862 1104 98-99 0.8597809899892468 1104 100-101 1.491219337725962 1105 1 0.06326489233807564 1105 2 0.07213036259629746 1105 3 0.09499304321416702 1105 4 0.17441385772031026 1105 5 0.0746794345069901 1105 6 0.01564671406066509 1105 7 0.08155886774470389 1105 8 0.09163912596891066 1105 9 -0.012165085725918345 1105 10-11 0.1089630823303267 1105 12-13 0.06079819842123868 1105 14-15 0.2921290732102477 1105 16-17 0.17973609110359945 1105 18-19 0.07325302746395579 1105 20-21 0.24302732660723336 1105 22-23 0.04092260342893894 1105 24-25 0.18617144338455915 1105 26-27 0.1169995685361016 1105 28-29 0.1271359566658461 1105 30-31 0.019259380928986047 1105 32-33 0.1746722216037 1105 34-35 0.1864208046938245 1105 36-37 0.24312804703254898 1105 38-39 0.2286233412782721 1105 40-41 0.23231323588509412 1105 42-43 0.10557957864049428 1105 44-45 0.22120194511101232 1105 46-47 0.35987748898080696 1105 48-49 0.17666975012019037 1105 50-51 0.10268859101623917 1105 52-53 0.26651061274106524 1105 54-55 0.3786932160651517 1105 56-57 0.31915406758713516 1105 58-59 0.17332938420896227 1105 60-61 0.1773762710727098 1105 62-63 0.035300461790875204 1105 64-65 0.18302044618081226 1105 66-67 0.22098269669015735 1105 68-69 0.1638596006752806 1105 70-71 0.4789762093028287 1105 72-73 0.2943933016213869 1105 74-75 0.3736782522487445 1105 76-77 0.2504949869628703 1105 78-79 0.4406383603878865 1105 80-81 0.36897338265583457 1105 82-83 0.5983963955723794 1105 84-85 0.37424136514745143 1105 86-87 0.3452831191692134 1105 88-89 0.42937501597372574 1105 90-91 0.6329716179575513 1105 92-93 0.8296342974510438 1105 94-95 0.8843508939139042 1105 96-97 0.6887827231677122 1105 98-99 1.0615771487509527 1105 100-101 1.7596838351662143 1106 1 0.0017402769817209673 1106 2 -0.008518781585628687 1106 3 -0.00961023148639839 1106 4 -0.03800698395487956 1106 5 0.08303761482981287 1106 6 0.04377024084337222 1106 7 -0.03378014315124034 1106 8 0.06748601545236 1106 9 0.006577809667746237 1106 10-11 0.06599207626705805 1106 12-13 0.05471349212592003 1106 14-15 0.10420981215052905 1106 16-17 0.06592922619162778 1106 18-19 0.038393772649151 1106 20-21 -0.021576784829726137 1106 22-23 0.17885961962691965 1106 24-25 0.17865918315545315 1106 26-27 0.16453078798756593 1106 28-29 0.1571212219418996 1106 30-31 0.1281361337386855 1106 32-33 0.20253707438911306 1106 34-35 0.1150818835939873 1106 36-37 0.009903591472436801 1106 38-39 0.05450308797163217 1106 40-41 0.1631212579422865 1106 42-43 0.2524411028476834 1106 44-45 0.10567721955035125 1106 46-47 0.219264446913364 1106 48-49 0.23893518331495756 1106 50-51 0.3327747748655909 1106 52-53 0.28294176872301335 1106 54-55 0.27763178532753585 1106 56-57 0.31792906508712804 1106 58-59 0.12606653265212486 1106 60-61 0.29988315373982033 1106 62-63 0.3645899093203582 1106 64-65 0.27522267516495447 1106 66-67 0.2289158761456207 1106 68-69 0.19729946483826666 1106 70-71 0.22410528099481297 1106 72-73 0.3233561158312135 1106 74-75 0.5383148127315209 1106 76-77 0.16879941207394467 1106 78-79 0.3558830853771191 1106 80-81 0.252842023204078 1106 82-83 0.40302456828293387 1106 84-85 0.527232493700776 1106 86-87 0.4340123818819954 1106 88-89 0.5750855174237266 1106 90-91 0.6506930826952342 1106 92-93 0.9534126112843353 1106 94-95 0.7488399030751474 1106 96-97 0.4529720378397819 1106 98-99 0.7016488631830242 1106 100-101 1.1138973131529042 1107 1 -0.1542596432122103 1107 2 -0.10868138645579961 1107 3 -0.1750980959756845 1107 4 -0.1147554850970991 1107 5 -0.13109304766033603 1107 6 -0.11412008566321674 1107 7 -0.15315605967408885 1107 8 -0.09850163455811156 1107 9 -0.04977325723967141 1107 10-11 -0.1574444729184279 1107 12-13 -0.0816815678782703 1107 14-15 -0.11150899115742874 1107 16-17 -0.14261201858356287 1107 18-19 -0.16134935155901786 1107 20-21 -0.1250144313486814 1107 22-23 -0.12334203036359526 1107 24-25 -0.269603039643151 1107 26-27 -0.11264367805993203 1107 28-29 -0.09432227037177654 1107 30-31 -0.22433386416640388 1107 32-33 -0.16831226172412528 1107 34-35 -0.2108780338067291 1107 36-37 -0.23543523517879805 1107 38-39 -0.24335761053099958 1107 40-41 -0.3715729351684587 1107 42-43 -0.28973590701834695 1107 44-45 -0.44329128697878417 1107 46-47 -0.6730706787313139 1107 48-49 -0.6374078032277524 1107 50-51 -0.42553054321147243 1107 52-53 -0.5374811036970115 1107 54-55 -0.4164151805453926 1107 56-57 -0.3684260918814317 1107 58-59 -0.42639099555433546 1107 60-61 -0.4547655499103129 1107 62-63 -0.33720971758625495 1107 64-65 -0.35254584732540195 1107 66-67 -0.21085049158079983 1107 68-69 -0.522111366840754 1107 70-71 -0.5123750717480178 1107 72-73 -0.2906787793734438 1107 74-75 -0.3301233824541754 1107 76-77 -0.611251564054065 1107 78-79 -0.27972189452172813 1107 80-81 -0.28804013315596677 1107 82-83 -0.20355494350342695 1107 84-85 -0.3236967482413071 1107 86-87 -0.2175228874136188 1107 88-89 -0.12811520482121708 1107 90-91 -0.09096967679919743 1107 92-93 -0.0082695011823688 1107 94-95 -0.2778108356298006 1107 96-97 -0.09363171504071843 1107 98-99 -0.24153869106016046 1107 100-101 0.2151791981723079 1108 1 0.015095807538592965 1108 2 -0.0220331509703513 1108 3 -6.252819679914978E-4 1108 4 -0.08705450390775127 1108 5 -0.048103943741004684 1108 6 -0.05394227897377846 1108 7 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-1.4093472396991302 2316 58-59 -1.3170127301415917 2316 60-61 -1.2948808943359964 2316 62-63 -1.4932884090097787 2316 64-65 -1.4196385247629024 2316 66-67 -1.4909666398003978 2316 68-69 -1.6921921481284343 2316 70-71 -1.67885887530079 2316 72-73 -1.5912092833522244 2316 74-75 -1.7614371835955644 2316 76-77 -1.7679994139053647 2316 78-79 -1.806890128586513 2316 80-81 -2.023573852310374 2316 82-83 -2.0774175751246773 2316 84-85 -2.366161180360912 2316 86-87 -2.446489610345857 2316 88-89 -2.9381746855703526 2316 90-91 -2.9921492443580746 2316 92-93 -3.6214115315657764 2316 94-95 -3.69386059047266 2316 96-97 -4.093609610708267 2316 98-99 -4.082080046256692 2316 100-101 -5.0701013259013195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 5.0 5 51.0 6 127.0 7 198.0 8 353.0 9 638.0 10 1131.0 11 1687.0 12 2482.0 13 3690.0 14 4383.0 15 5676.0 16 7105.0 17 9648.0 18 10082.0 19 7877.0 20 7830.0 21 8636.0 22 9369.0 23 10719.0 24 12370.0 25 14794.0 26 17679.0 27 21527.0 28 26666.0 29 32686.0 30 39962.0 31 48739.0 32 60571.0 33 77518.0 34 103507.0 35 151032.0 36 235460.0 37 326051.0 38 183848.0 39 9271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.34239058734467 18.165805907499415 14.195722612904275 16.29608089225165 2 13.28221542729597 28.00556223733497 32.45046691418302 26.261755421186038 3 32.32448402748918 23.844067451416432 30.8977329960946 12.933715524999794 4 11.407265311289883 15.17381647644237 44.237332217766685 29.181585994501063 5 9.765888128584354 43.965074155494314 34.61800717644914 11.65103053947219 6 24.263437027822196 38.69353475916696 15.4989225057315 21.54410570727935 7 22.799531021651717 36.78996155148166 21.589930176169624 18.820577250697 8 21.302323149200618 23.979132665277714 20.403792009203425 34.314752176318244 9 18.308801875913396 26.965566971153976 28.018910506050755 26.70672064688187 10-11 25.137851836969478 22.162116787718492 26.928377593623654 25.771653781688375 12-13 35.61008468582029 21.844579364402232 23.4985949915094 19.04674095826808 14-15 25.594090913782246 25.94403982509435 32.98172160277427 15.48014765834913 16-17 17.633400615204234 28.721732755307865 32.77457385185901 20.870292777628887 18-19 16.591313166897397 37.068245432002264 27.847791205555083 18.492650195545256 20-21 21.927077169506497 27.60198352520896 22.97918220524408 27.49175710004046 22-23 18.266073654921108 27.70268038740254 26.004009985055443 28.02723597262091 24-25 26.626665437341572 27.420027357070314 24.94364828825655 21.00965891733156 26-27 25.420229645266318 20.986574669114308 34.76566907852911 18.82752660709027 28-29 17.886404857118478 33.458226799470474 29.363714176411822 19.291654166999226 30-31 19.865010472198446 28.48912735349243 26.751375422121797 24.894486752187326 32-33 21.09191590315487 28.770335468138917 28.396652749605742 21.741095879100463 34-35 26.124239509322678 24.355758949397867 27.00524585471514 22.514755686564307 36-37 23.8494059263551 23.090910091786046 33.937579432879346 19.12210454897951 38-39 23.03526914203825 26.340119014645442 32.200738723456965 18.423873119859344 40-41 20.153604482905962 29.399047575142273 30.423169203691074 20.024178738260687 42-43 17.714383221856558 31.55937213381066 24.79673062619421 25.929514018138573 44-45 21.737393836998447 25.879157115565825 25.332908113679515 27.050540933756213 46-47 25.42165822994711 29.283547399180797 25.403011898561207 19.891782472310886 48-49 23.914521304996395 26.561510918088192 27.92978103274601 21.594186744169406 50-51 17.565845559574587 29.08726175084958 34.3113179570417 19.03557473253413 52-53 20.629439675458176 32.26279988881029 26.219371227737977 20.888389207993548 54-55 16.27467021519611 26.09118656476112 30.362082109742033 27.272061110300733 56-57 23.583845016967437 25.956671794970593 26.33252188703236 24.126961301029606 58-59 28.211123113866392 24.75178378527147 25.330438910944906 21.706654189917227 60-61 21.39292682247148 26.339857520854615 33.03683148178793 19.230384174885977 62-63 21.410347798086107 26.278440757080773 30.82393908785913 21.48727235697399 64-65 19.439832554568042 33.01405217807728 27.96653843586866 19.57957683148602 66-67 20.828645877412622 26.3740868491833 25.77220030729069 27.025066966113393 68-69 17.63363744796915 29.08223067552119 28.159875377104328 25.12425649940534 70-71 24.675960456097958 28.163952518694458 24.03514034947694 23.124946675730644 72-73 21.8770211618223 22.880996744123323 31.572164593786038 23.66981750026834 74-75 18.101422072256792 33.672246334730545 26.44904401626012 21.777287576752546 76-77 21.627707118807532 24.963411378725382 26.63079850855514 26.778082993911944 78-79 20.92878524176372 25.97653112091437 29.996700774715894 23.097982862606017 80-81 23.83388423610748 26.65807515212898 27.250456180523635 22.257584431239902 82-83 20.59710108630137 25.198091059962625 31.399325155569258 22.805482698166745 84-85 19.698948376602825 27.066435847119664 29.261710009550207 23.972905766727308 86-87 20.332398215018415 28.641526763527146 27.781971245025687 23.244103776428748 88-89 20.214037611529026 27.075339829706845 25.4123455971212 27.29827696164293 90-91 21.230349443318353 22.944139941610466 28.27626623115533 27.549244383915855 92-93 22.973058514956943 28.51627112673149 28.05534302298379 20.455327335327773 94-95 21.079393300545217 27.121101823896428 27.716235072644857 24.083269802913502 96-97 14.864175411037914 27.365590232588062 31.799167930115214 25.971066426258815 98-99 20.03723066083563 29.00062750624066 24.85750379118354 26.104638041740174 100-101 19.339210943404925 25.049806707658796 27.66253018418935 27.94845216474693 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6642.0 1 4022.5 2 1333.5 3 1158.0 4 856.5 5 793.0 6 1417.0 7 2573.0 8 3397.0 9 3375.5 10 2519.0 11 1661.5 12 1466.5 13 1431.0 14 1375.0 15 1277.0 16 1198.5 17 1244.5 18 1367.0 19 1496.5 20 1744.0 21 2088.0 22 2447.5 23 2800.0 24 3157.5 25 3963.0 26 5426.0 27 6851.5 28 7512.5 29 8187.5 30 9390.5 31 10693.0 32 12964.0 33 15998.0 34 19145.5 35 21304.0 36 23197.5 37 25267.5 38 24103.5 39 21605.0 40 20142.0 41 18871.0 42 17958.5 43 17849.5 44 19848.0 45 23178.5 46 41072.5 47 131817.5 48 328270.5 49 311765.5 50 125528.5 51 60570.5 52 31292.0 53 13398.5 54 6291.0 55 4488.0 56 3583.0 57 2775.0 58 2218.0 59 1830.0 60 1459.0 61 1169.0 62 856.5 63 538.5 64 290.0 65 191.5 66 142.0 67 125.0 68 116.0 69 85.0 70 65.5 71 61.5 72 65.5 73 62.0 74 54.0 75 49.5 76 44.0 77 29.5 78 20.5 79 20.0 80 14.5 81 8.0 82 5.5 83 7.0 84 6.0 85 3.5 86 2.0 87 0.5 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3866181541958975 2 0.0 3 0.0 4 0.0 5 4.8163856190982076E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011696936503524217 16-17 0.0023049845462827134 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023393873007048435 36-37 0.006949356393270271 38-39 0.013038643925987291 40-41 0.001995359756483543 42-43 3.4402754422130057E-4 44-45 3.784302986434306E-4 46-47 2.0641652653278033E-4 48-49 2.752220353770404E-4 50-51 6.19249579598341E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.376110176885202E-4 60-61 0.002511401072815494 62-63 0.0 64-65 2.0641652653278033E-4 66-67 0.0017201377211065027 68-69 0.0018921514932171528 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12106329281147565 78-79 3.440275442213005E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14039764079671274 88-89 0.41992002047651944 90-91 0.3201864354067644 92-93 0.0 94-95 0.0 96-97 5.848468251762109E-4 98-99 0.0 100-101 2.4081928095491038E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1453372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.935535021844025 #Duplication Level Percentage of deduplicated Percentage of total 1 72.50066884581413 11.553369474995872 2 13.105293338331666 4.176797219290469 3 4.918082498723234 2.3511682769616673 4 2.420610522352545 1.5429489501277256 5 1.4090493708386236 1.1226977798253088 6 0.9265563808038455 0.8859102993607638 7 0.6564289628714299 0.7322382709033295 8 0.4908765913182774 0.625790488988466 9 0.388554175398962 0.5572636802958495 >10 2.596501358821248 8.10022402176545 >50 0.31185530588314375 3.4109523203063152 >100 0.2132380940024149 6.378571131546715 >500 0.026816961111865566 2.855131205096708 >1k 0.01903139175680782 7.435417008662032 >5k 0.006055442831711578 6.785373738129549 >10k+ 0.010380759140076993 41.48614613374376 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 101206 6.963530328092188 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 54657 3.7607026969007245 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 39016 2.684515733067652 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 32043 2.2047349198966266 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 31727 2.1829923791018406 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 27461 1.8894680783722269 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 25800 1.7751821281819107 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 25752 1.771879463757386 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 25365 1.7452517318346576 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 24158 1.662203482659636 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 23850 1.6410113859356035 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 23599 1.6237412032156944 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 20526 1.4123018745372828 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 17687 1.2169630349284284 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17112 1.177399867342979 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 14398 0.990661716339657 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 13868 0.9541947966521991 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 13711 0.9433923317636502 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 13474 0.9270854261675607 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 12700 0.8738299623221033 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12122 0.834060378210121 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11146 0.7669062015781232 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10459 0.7196368170021165 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 10055 0.6918393914290354 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9760 0.6715417663199786 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9329 0.6418865920081025 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8694 0.5981950938919973 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8607 0.5922090146225467 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 8200 0.5642051725229328 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7721 0.5312473337865323 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7294 0.5018673815100332 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 7118 0.4897576119534434 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5817 0.400241644947061 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5371 0.369554388002521 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5264 0.3621921985561852 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5163 0.35524284216291496 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5059 0.3480870692431119 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5047 0.34726140313698073 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4944 0.34017443572602196 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4908 0.3376974374076286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4692 0.3228354474972684 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4656 0.32035844917887507 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4571 0.31450998092711296 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 4527 0.31148253853796554 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4125 0.28382272398257297 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3925 0.27006162221372093 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3435 0.23634692288003345 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3341 0.22987920504867304 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3301 0.22712698469490264 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3280 0.22568206900917318 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3229 0.22217298805811586 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3215 0.22120971093429623 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2885 0.19850389301569038 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2820 0.1940315349408135 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2563 0.17634851916783864 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2556 0.1758668806059288 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2513 0.17290824372562563 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2481 0.1707064674426093 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2424 0.1667845534384865 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 2229 0.15336747921385577 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2118 0.14573006773214292 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1992 0.13706057361776613 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1867 0.1284598850122336 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1757 0.120891279039365 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1721 0.11841428072097164 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1551 0.10671734421744741 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1493 0.10272662470448034 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1480 0.10183215308950495 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0641652653278033E-4 0.0 10-11 0.0 0.0 0.0 3.096247897991705E-4 0.0 12-13 0.0 0.0 0.0 4.472358074876907E-4 0.0 14-15 0.0 0.0 0.0 6.19249579598341E-4 0.0 16-17 0.0 0.0 0.0 0.0010320826326639017 0.0 18-19 0.0 0.0 0.0 0.0017545404755286327 0.0 20-21 0.0 0.0 0.0 0.002683414844926144 0.0 22-23 0.0 0.0 0.0 0.004816385619098208 0.0 24-25 0.0 0.0 0.0 0.006570926094626841 0.0 26-27 0.0 0.0 0.0 0.010011201536839844 0.0 28-29 0.0 0.0 0.0 0.026146093360818842 0.0 30-31 0.0 0.0 0.0 0.06285383232923161 0.0 32-33 0.0 0.0 0.0 0.14779423299747071 0.0 34-35 0.0 0.0 0.0 0.28574927823021223 0.0 36-37 0.0 0.0 0.0 0.4527058454408094 0.0 38-39 0.0 0.0 0.0 0.6647300209443969 0.0 40-41 0.0 0.0 0.0 0.8871094255290455 0.0 42-43 0.0 0.0 0.0 1.1304057048023493 0.0 44-45 0.0 0.0 0.0 1.4124050828005492 0.0 46-47 0.0 0.0 0.0 1.784195649840509 0.0 48-49 0.0 0.0 0.0 2.1177647567174818 0.0 50-51 0.0 0.0 0.0 2.4574575538815937 0.0 52-53 0.0 0.0 0.0 2.7682520373311172 0.0 54-55 0.0 0.0 0.0 3.1265567246376014 0.0 56-57 0.0 0.0 0.0 3.6603498622513713 0.0 58-59 0.0 0.0 0.0 4.155783928684466 0.0 60-61 0.0 0.0 0.0 4.782842933536631 0.0 62-63 0.0 0.0 0.0 5.338172195418654 0.0 64-65 0.0 0.0 0.0 5.812104540337918 0.0 66-67 0.0 0.0 0.0 6.407478608367301 0.0 68-69 0.0 0.0 0.0 7.112459851985589 0.0 70-71 0.0 0.0 0.0 7.818989219552874 0.0 72-73 0.0 0.0 0.0 8.489292486713655 0.0 74-75 0.0 0.0 0.0 9.309144527347438 0.0 76-77 0.0 0.0 0.0 9.92808448215598 0.0 78-79 0.0 0.0 0.0 10.984558667705171 0.0 80-81 0.0 0.0 0.0 12.04664050222517 0.0 82-83 0.0 0.0 0.0 12.854795606355427 0.0 84-85 0.0 0.0 0.0 13.948355961171675 0.0 86-87 0.0 0.0 0.0 15.05622786182753 0.0 88-89 0.0 0.0 0.0 16.111497950971945 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTGCA 30 1.308051E-4 63.26753 2 GTAAGCG 505 0.0 41.344563 94-95 GCAAGCG 45 2.4308652E-5 31.634855 94-95 GCCACGA 30 0.00397143 31.634853 92-93 CTCCGAC 235 0.0 31.298313 94-95 GCTTATG 335 0.0 31.292963 1 AGTACTC 210 4.3655746E-11 27.114656 5 GCGTATC 35 0.008436309 27.114656 78-79 CTTATGT 370 0.0 26.93145 2 ATGCCGC 55 9.546527E-5 25.883062 94-95 AAGCAGG 1160 0.0 25.771498 94-95 CGTGCAC 95 0.0012341667 24.974026 3 GACGTGG 115 1.2910608E-4 24.756859 7 AGTGGAC 115 1.2910608E-4 24.756859 8 CTTTGCG 1415 0.0 24.47984 2 GCAGAGA 2185 0.0 24.106194 94-95 GCTTTGC 1445 0.0 23.742884 1 CGTGGAA 100 0.0016635587 23.726141 9 TTAGGCA 220 2.1955202E-9 23.725325 4 GCTTTCC 410 0.0 23.244219 1 >>END_MODULE