##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939043_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2664365 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84771043006495 33.0 31.0 34.0 30.0 34.0 2 31.897617255893994 33.0 31.0 34.0 30.0 34.0 3 31.94782283958842 34.0 31.0 34.0 30.0 34.0 4 35.48164084125111 37.0 35.0 37.0 33.0 37.0 5 35.534208713896184 37.0 35.0 37.0 33.0 37.0 6 35.63959067169851 37.0 35.0 37.0 33.0 37.0 7 35.567424508278705 37.0 35.0 37.0 33.0 37.0 8 35.57018426529398 37.0 35.0 37.0 33.0 37.0 9 37.16305461151156 39.0 37.0 39.0 33.0 39.0 10-11 37.05373456714827 39.0 37.0 39.0 33.0 39.0 12-13 36.95865318753249 39.0 37.0 39.0 33.0 39.0 14-15 38.1303357460408 40.0 38.0 41.0 33.0 41.0 16-17 38.07249738680699 40.0 38.0 41.0 33.0 41.0 18-19 38.03305496806931 40.0 38.0 41.0 33.0 41.0 20-21 38.1331153952255 40.0 38.0 41.0 33.0 41.0 22-23 38.06413291722418 40.0 38.0 41.0 33.0 41.0 24-25 37.96691650731037 40.0 38.0 41.0 33.0 41.0 26-27 37.82868694792192 40.0 37.5 41.0 32.5 41.0 28-29 37.70868236896972 40.0 37.0 41.0 32.0 41.0 30-31 37.52341815029097 40.0 37.0 41.0 32.0 41.0 32-33 37.29209042304639 40.0 36.5 41.0 31.5 41.0 34-35 36.934674115596025 40.0 36.0 41.0 30.5 41.0 36-37 36.643865423843955 39.0 35.5 41.0 30.0 41.0 38-39 36.388992686812806 39.0 35.0 41.0 30.0 41.0 40-41 36.25911690027455 39.0 35.0 41.0 30.0 41.0 42-43 36.02080139170121 39.0 35.0 41.0 29.5 41.0 44-45 35.90315666209397 39.0 35.0 41.0 28.5 41.0 46-47 35.62958584878573 39.0 35.0 41.0 27.0 41.0 48-49 35.33318576846641 39.0 35.0 41.0 26.0 41.0 50-51 34.166196635971424 38.0 34.0 40.0 23.5 40.5 52-53 34.28797931214379 38.0 33.5 39.5 24.0 40.5 54-55 34.79716667949023 39.0 35.0 40.0 24.0 41.0 56-57 34.678228771208154 38.5 34.5 40.0 23.5 41.0 58-59 34.46933509485375 38.0 34.0 40.0 23.0 41.0 60-61 34.11058863931931 38.0 34.0 40.0 22.5 41.0 62-63 33.74762635749981 37.0 33.5 40.0 22.0 41.0 64-65 33.35412058783237 37.0 33.0 39.5 20.5 41.0 66-67 32.899705558360054 36.0 33.0 39.0 19.5 41.0 68-69 32.43967174167203 36.0 32.0 39.0 17.5 40.5 70-71 31.94957335049815 35.0 31.5 38.5 14.0 40.0 72-73 31.491034073784935 35.0 31.0 37.0 10.0 39.5 74-75 31.036260609938957 35.0 31.0 37.0 7.5 39.0 76-77 30.616400718370045 35.0 31.0 36.0 4.0 39.0 78-79 30.212684448264408 34.5 30.0 36.0 2.0 38.5 80-81 29.74496193276822 34.0 30.0 35.5 2.0 37.0 82-83 29.268796880307313 34.0 29.0 35.0 2.0 37.0 84-85 28.842307266459365 34.0 29.0 35.0 2.0 36.0 86-87 28.434000033779157 34.0 29.0 35.0 2.0 36.0 88-89 27.9392603866212 34.0 27.5 35.0 2.0 35.5 90-91 27.454359481527494 34.0 26.5 35.0 2.0 35.0 92-93 26.937979593636758 33.0 25.5 35.0 2.0 35.0 94-95 26.380079868936875 33.0 25.0 35.0 2.0 35.0 96-97 25.700683089591706 33.0 22.0 35.0 2.0 35.0 98-99 24.88001043400585 32.0 10.5 35.0 2.0 35.0 100-101 21.8061326057053 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04994493375843945 1101 2 0.195682708721705 1101 3 0.32279246223101055 1101 4 0.15957983920652907 1101 5 0.08751173930679101 1101 6 0.22866658619579994 1101 7 0.23815270042860703 1101 8 0.1514172427910978 1101 9 0.12350302948503611 1101 10-11 0.1292432565352044 1101 12-13 0.18392559508692585 1101 14-15 0.34464992912688786 1101 16-17 0.30630059572457924 1101 18-19 0.3324762937406618 1101 20-21 0.4329254157373015 1101 22-23 0.4606555029550208 1101 24-25 0.4205574904334526 1101 26-27 0.4253567307392885 1101 28-29 0.39180617585787303 1101 30-31 0.337840302258833 1101 32-33 0.3091280511962111 1101 34-35 0.29991136612672165 1101 36-37 0.24932588247053644 1101 38-39 0.3356512971590533 1101 40-41 0.3019681705158561 1101 42-43 0.3759103547498839 1101 44-45 0.41601660985439537 1101 46-47 0.315003218187492 1101 48-49 0.3016456224214039 1101 50-51 0.32021772273841975 1101 52-53 0.32996407210219303 1101 54-55 0.3662926107975082 1101 56-57 0.39435483586641595 1101 58-59 0.2805292452339003 1101 60-61 0.3382820776326767 1101 62-63 0.26940270612033146 1101 64-65 0.06160457058354041 1101 66-67 0.14056644369274807 1101 68-69 0.17511842771877184 1101 70-71 0.08260835533654642 1101 72-73 0.06644124675083773 1101 74-75 -0.0854496630478998 1101 76-77 -0.11125456758644603 1101 78-79 -0.12882102549922436 1101 80-81 -0.07699635803036742 1101 82-83 -0.05168074526560318 1101 84-85 -0.08225238792293865 1101 86-87 -0.16313812330992405 1101 88-89 -0.3944949589880551 1101 90-91 -0.3310029892601456 1101 92-93 -0.4817563843573076 1101 94-95 -0.4249231338688091 1101 96-97 -0.5768802602287302 1101 98-99 -0.9234686805726575 1101 100-101 -0.5214274918975583 1102 1 0.1569577302956553 1102 2 0.2800698269102213 1102 3 0.32294796633415856 1102 4 0.19278890418498662 1102 5 0.31605757017769776 1102 6 0.2849449041338872 1102 7 0.3187122588374294 1102 8 0.2056979570440376 1102 9 0.29390010803181355 1102 10-11 0.38614919038288065 1102 12-13 0.3585265128647208 1102 14-15 0.3724033637099211 1102 16-17 0.5050221900835297 1102 18-19 0.47084998393061284 1102 20-21 0.36396440577694733 1102 22-23 0.40135834776934587 1102 24-25 0.47372623228719846 1102 26-27 0.5602273621196829 1102 28-29 0.5123852717887232 1102 30-31 0.4230503103814769 1102 32-33 0.3730439481007082 1102 34-35 0.40597865723849935 1102 36-37 0.4354182498838952 1102 38-39 0.5542737062110774 1102 40-41 0.42694978662513705 1102 42-43 0.4834488630978555 1102 44-45 0.45681754706176747 1102 46-47 0.5216863865043209 1102 48-49 0.560216900644896 1102 50-51 0.4660949075542078 1102 52-53 0.34111297419600106 1102 54-55 0.477203129486341 1102 56-57 0.39210795336949644 1102 58-59 0.27945977504075614 1102 60-61 0.3597917357530278 1102 62-63 0.30583890288800575 1102 64-65 0.23890117391517407 1102 66-67 0.27848778897526927 1102 68-69 0.3492140414910949 1102 70-71 0.36859305049453894 1102 72-73 0.15710030754653204 1102 74-75 0.15689420543543164 1102 76-77 0.1444776644675052 1102 78-79 0.03267049160015745 1102 80-81 0.17154743543320095 1102 82-83 0.12071326232812396 1102 84-85 0.05361620200228501 1102 86-87 -0.17687358823008736 1102 88-89 -0.2831339506468318 1102 90-91 -0.3526281791707966 1102 92-93 -0.3480407335608291 1102 94-95 -0.44351683706578626 1102 96-97 -0.4797176872666107 1102 98-99 -0.5866135672199491 1102 100-101 -0.40421821373344 1103 1 0.22586866866742739 1103 2 0.09553875611996432 1103 3 0.30718443234670545 1103 4 0.26927165479324344 1103 5 0.16238215351609853 1103 6 0.3038397326261517 1103 7 0.267007482342585 1103 8 0.16871582043496147 1103 9 0.4317143857755923 1103 10-11 0.4324140962430931 1103 12-13 0.3867536729732777 1103 14-15 0.4302157940524225 1103 16-17 0.48159928649558736 1103 18-19 0.5290444750948353 1103 20-21 0.4993678586273447 1103 22-23 0.4429200638058717 1103 24-25 0.4939423579726281 1103 26-27 0.6015238730642665 1103 28-29 0.6154572603219961 1103 30-31 0.4514980103500008 1103 32-33 0.4902168094719812 1103 34-35 0.5090119753062865 1103 36-37 0.44020080703850084 1103 38-39 0.5907925571342432 1103 40-41 0.5501608976461867 1103 42-43 0.5180794470117576 1103 44-45 0.5392574913222603 1103 46-47 0.6375574547818417 1103 48-49 0.5397066936683999 1103 50-51 0.3532307634852714 1103 52-53 0.4382525924762888 1103 54-55 0.48754891437182835 1103 56-57 0.40271193538047356 1103 58-59 0.42397662388078317 1103 60-61 0.4569963676980109 1103 62-63 0.506508053921003 1103 64-65 0.425869834964665 1103 66-67 0.33530584798133134 1103 68-69 0.39840059389582905 1103 70-71 0.2685418332360925 1103 72-73 0.15129914668955635 1103 74-75 0.054948219801257636 1103 76-77 0.11085842298952997 1103 78-79 0.04835246299008844 1103 80-81 0.1082715775567813 1103 82-83 0.2895939397941518 1103 84-85 0.2058627534073203 1103 86-87 0.2789062278808707 1103 88-89 0.2568338329714841 1103 90-91 0.13765033599140253 1103 92-93 0.15309793433788954 1103 94-95 0.03411170761430782 1103 96-97 -0.07793957198066437 1103 98-99 -0.01559441301226272 1103 100-101 0.28532735257104136 1104 1 0.21677218079608096 1104 2 0.3246887839199246 1104 3 0.30166110637780363 1104 4 0.3075479370915346 1104 5 0.2693678442018381 1104 6 0.24420947879982435 1104 7 0.24372784671543712 1104 8 0.33401710003325746 1104 9 0.3982582637170893 1104 10-11 0.355971864832739 1104 12-13 0.37460285584104014 1104 14-15 0.4917897119538637 1104 16-17 0.4041498064282223 1104 18-19 0.5250440637620528 1104 20-21 0.44979717472158853 1104 22-23 0.46428970197968056 1104 24-25 0.4998473500993015 1104 26-27 0.6139067732203998 1104 28-29 0.5413316924894289 1104 30-31 0.4834486344158293 1104 32-33 0.5799616064789248 1104 34-35 0.6326573104458362 1104 36-37 0.5722663809510706 1104 38-39 0.6189573529145136 1104 40-41 0.497767800836975 1104 42-43 0.5980614237024469 1104 44-45 0.6615004949982577 1104 46-47 0.6836921432689209 1104 48-49 0.775813378859489 1104 50-51 0.8049478278625202 1104 52-53 0.7551149199931899 1104 54-55 0.8565489223718217 1104 56-57 0.8514444204038227 1104 58-59 0.8629234552850065 1104 60-61 0.7537693053340959 1104 62-63 0.7161340244197021 1104 64-65 0.7174812794827332 1104 66-67 0.7660436903308749 1104 68-69 0.769542515706604 1104 70-71 0.7760998231587735 1104 72-73 0.761666749532754 1104 74-75 0.5973787465605547 1104 76-77 0.6674885974826275 1104 78-79 0.6410828393495898 1104 80-81 0.4736828403417661 1104 82-83 0.6156861716711752 1104 84-85 0.642957587280879 1104 86-87 0.6031290455837812 1104 88-89 0.47962795257932456 1104 90-91 0.5784431649342956 1104 92-93 0.4183534139972487 1104 94-95 0.5472916900409963 1104 96-97 0.5640506553656977 1104 98-99 0.6144834968838531 1104 100-101 0.9958742385085273 1105 1 0.16829637398320685 1105 2 0.2493193746547817 1105 3 0.24589806586277874 1105 4 0.1917038153224624 1105 5 0.24351255536187466 1105 6 0.19236643214130567 1105 7 0.2246974300460991 1105 8 0.25648835042522933 1105 9 0.3354121325152022 1105 10-11 0.2925713797530918 1105 12-13 0.35826869484323254 1105 14-15 0.4168564264032213 1105 16-17 0.24523766791814694 1105 18-19 0.28134457955913916 1105 20-21 0.34175712365384214 1105 22-23 0.17354877162508586 1105 24-25 0.366445649120827 1105 26-27 0.2872746690114738 1105 28-29 0.2750962432788029 1105 30-31 0.33844117903611703 1105 32-33 0.3703482463104848 1105 34-35 0.4547317938488149 1105 36-37 0.2987775352230386 1105 38-39 0.466404445316428 1105 40-41 0.5029310512545422 1105 42-43 0.19043685806631316 1105 44-45 0.3592163650378737 1105 46-47 0.247223257480222 1105 48-49 0.26618569988237795 1105 50-51 0.24989224848673075 1105 52-53 0.2654798976270456 1105 54-55 0.26567888275911145 1105 56-57 0.20093990237972292 1105 58-59 0.13118377602632592 1105 60-61 0.1684874407852206 1105 62-63 0.2082254824103984 1105 64-65 0.37684962003680056 1105 66-67 0.303554249452354 1105 68-69 0.23885714872913866 1105 70-71 0.1775902591544991 1105 72-73 0.14123140975302206 1105 74-75 0.04235508452923398 1105 76-77 0.026652235067981422 1105 78-79 0.05013792596638211 1105 80-81 0.2150996344644973 1105 82-83 0.22013700136214354 1105 84-85 0.026174103666715354 1105 86-87 0.12048720138522029 1105 88-89 0.26641825162644395 1105 90-91 0.22268564396004464 1105 92-93 0.314581407828161 1105 94-95 0.2314894847844755 1105 96-97 0.11031056654108795 1105 98-99 0.26536606345431935 1105 100-101 0.5317085370948824 1106 1 0.057465840952282576 1106 2 0.11316551099600503 1106 3 0.07501640676893473 1106 4 0.18741726663512992 1106 5 0.1436490938088255 1106 6 0.16183112557456525 1106 7 0.08038160144736395 1106 8 0.12496920849351056 1106 9 0.1253962788844163 1106 10-11 0.1439052845251112 1106 12-13 0.18124925328547903 1106 14-15 0.20303001228212025 1106 16-17 0.2054412619134922 1106 18-19 0.11495844883115325 1106 20-21 0.11832826397743901 1106 22-23 0.2368055170036385 1106 24-25 0.17020880042817765 1106 26-27 0.17938616023887022 1106 28-29 0.028786574081117067 1106 30-31 0.22221965299141289 1106 32-33 0.22835888113841207 1106 34-35 0.048570117156693016 1106 36-37 0.10521115098867995 1106 38-39 0.35924793432887725 1106 40-41 0.20850409553093385 1106 42-43 0.14490014818775876 1106 44-45 0.23253447686285966 1106 46-47 0.22214607533035036 1106 48-49 0.23446668083526134 1106 50-51 0.06645663774267518 1106 52-53 0.18002389714158085 1106 54-55 0.21474852495253316 1106 56-57 0.1874100801072629 1106 58-59 0.23083294807235433 1106 60-61 0.3653428844972453 1106 62-63 0.366344210175491 1106 64-65 0.10187408317521118 1106 66-67 0.0728361330760876 1106 68-69 0.09347366532993817 1106 70-71 0.13751053587241557 1106 72-73 0.08602550218954619 1106 74-75 0.18149345107427806 1106 76-77 0.18571156102318298 1106 78-79 0.2356299574309091 1106 80-81 0.22174526409637352 1106 82-83 0.23606648683976061 1106 84-85 0.21445265726942964 1106 86-87 0.37898975389563105 1106 88-89 0.3817909636592738 1106 90-91 0.4915207434434734 1106 92-93 0.3504610567726445 1106 94-95 0.38424225158439285 1106 96-97 0.35949778350826733 1106 98-99 0.4997096548705997 1106 100-101 0.6935145614745828 1107 1 0.08730172623371146 1107 2 0.058249567961247806 1107 3 0.11480503665657338 1107 4 -5.977421719620679E-4 1107 5 0.09112738485146821 1107 6 0.09276414759669649 1107 7 0.011749831825497381 1107 8 0.12947302685871165 1107 9 0.13278688132452743 1107 10-11 0.037171023335403675 1107 12-13 0.20237964817824405 1107 14-15 0.22326970034358595 1107 16-17 0.17557626472176224 1107 18-19 0.0743026368882127 1107 20-21 0.15507572844767736 1107 22-23 0.20652780702295814 1107 24-25 0.08401754988049248 1107 26-27 0.10963711078685634 1107 28-29 0.12437435531246166 1107 30-31 0.2919732603687848 1107 32-33 0.34702211514817805 1107 34-35 0.24051788039158595 1107 36-37 0.29142548522280975 1107 38-39 0.3999674101968651 1107 40-41 0.20558966984422256 1107 42-43 0.2724657455553299 1107 44-45 0.03800883926791698 1107 46-47 0.16229948627583468 1107 48-49 0.3503920720081837 1107 50-51 0.3460930035176517 1107 52-53 0.3551235098649528 1107 54-55 0.4720622307810842 1107 56-57 0.34295207624332136 1107 58-59 0.4551230144416252 1107 60-61 0.34908153011708265 1107 62-63 0.3608338254571848 1107 64-65 0.24039266921855074 1107 66-67 0.21390921573332378 1107 68-69 0.3782029980790682 1107 70-71 0.15986304287839204 1107 72-73 0.46211109708702836 1107 74-75 0.41492289291956297 1107 76-77 0.5811208480166279 1107 78-79 0.6007715324734733 1107 80-81 0.6099518527980088 1107 82-83 0.6002448693349791 1107 84-85 0.6889138663841088 1107 86-87 0.671454032993573 1107 88-89 0.5812193116024709 1107 90-91 0.4693761366453977 1107 92-93 0.5230242575536685 1107 94-95 0.6221724241680313 1107 96-97 0.6547548946069632 1107 98-99 0.43117330762138195 1107 100-101 0.3336276862411722 1108 1 0.24334548989676463 1108 2 0.2725020858532048 1108 3 0.245505138554325 1108 4 0.2203875088391598 1108 5 0.22700960840705875 1108 6 0.28470434962657265 1108 7 0.35632807837615843 1108 8 0.32564355671206613 1108 9 0.30397794783937115 1108 10-11 0.3725320870222504 1108 12-13 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-1.9297974902726551 2316 80-81 -1.8308693174361181 2316 82-83 -1.7758435575329656 2316 84-85 -1.8475170549801305 2316 86-87 -1.6701913435130784 2316 88-89 -1.8731541466962867 2316 90-91 -1.9974578673634973 2316 92-93 -2.0712886646972244 2316 94-95 -2.0643852671923817 2316 96-97 -2.252182713619991 2316 98-99 -2.252382802725588 2316 100-101 -2.0845603151077547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7159.0 3 1850.0 4 1831.0 5 2078.0 6 2414.0 7 3131.0 8 3857.0 9 4799.0 10 6234.0 11 8654.0 12 12209.0 13 17973.0 14 16107.0 15 17247.0 16 21600.0 17 29329.0 18 25152.0 19 17247.0 20 17287.0 21 18415.0 22 19902.0 23 22284.0 24 25184.0 25 28988.0 26 33614.0 27 39218.0 28 46149.0 29 55142.0 30 65803.0 31 79817.0 32 99153.0 33 126125.0 34 168905.0 35 241455.0 36 360435.0 37 524307.0 38 432511.0 39 60798.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29538174318701 18.797725194007302 13.855523536364625 22.05136952644106 2 15.425318314917181 21.9542723412088 38.438843529365364 24.181565814508662 3 21.01437895924797 24.765929598175074 30.655164200546547 23.56452724203041 4 12.543586341061685 16.284243995595247 38.24984380113094 32.92232586221212 5 12.833100369735776 38.41979288784682 34.286229596897826 14.460877145519586 6 30.570182652825938 36.27906924343921 17.25073661235676 15.900011491378086 7 26.95389163493958 34.183574218852684 21.25394413579018 17.60859001041755 8 25.62929225065561 32.756921022400164 20.32381037504605 21.289976351898176 9 25.63448516736708 17.61821602803159 21.94118022616057 34.80611857844076 10-11 25.133070987529717 25.728954110014364 27.371397572565865 21.76657732989005 12-13 27.17323584252004 23.93795663611629 27.469050609974783 21.419756911388887 14-15 23.191103119721383 25.32044421311474 27.804196681320054 23.684255985843823 16-17 21.2058349079393 28.490963861642975 28.102712315499772 22.20048891491795 18-19 20.799507549289668 28.11761354449774 30.024207527863254 21.058671378349334 20-21 22.550995631454207 25.980087183126443 29.233839732602856 22.235077452816494 22-23 21.740365952744156 26.16596679296823 29.719977897539092 22.373689356748518 24-25 22.770037886543236 26.143499274013436 29.524023299190986 21.56243954025234 26-27 22.223271967085175 25.380457903717947 31.096130855392843 21.300139273804035 28-29 21.056612420492527 27.66134159500468 30.119933849030417 21.162112135472377 30-31 21.63258874567496 26.28704258962275 29.633095083062056 22.447273581640236 32-33 21.640242293582375 26.651073391368364 29.963070041099016 21.74561427395025 34-35 22.744356124837456 26.144885866764767 29.549557453315295 21.561200555082486 36-37 22.310239924256283 25.59562361257542 30.57186882908438 21.52226763408392 38-39 21.769872025003696 26.598518422426093 30.28404068530882 21.34756886726139 40-41 21.797466598095802 26.960773593983056 29.646367377963717 21.595392429957425 42-43 21.312845227159965 27.25804562792567 28.59638281233352 22.832726332580844 44-45 21.96567134229231 26.24932294707747 28.849262747831546 22.935742962798678 46-47 22.71348886083579 27.205758940543223 28.571606380366614 21.50914581825437 48-49 22.49488328263613 26.87910513889877 28.601106165354423 22.02490541311068 50-51 20.903676574462946 27.5274480805912 29.68891075099412 21.879964593951737 52-53 22.0220335763926 28.19206491740191 27.742479770365858 22.043421735839633 54-55 21.167854578674238 26.911756302427502 28.60942724303769 23.31096187586057 56-57 22.25369804815311 26.95599034682677 28.166012103988635 22.62429950103148 58-59 23.27217987280407 26.62299235636729 27.935860976186817 22.168966794641822 60-61 21.99908867995782 26.951178421991685 29.551738329789313 21.497994568261188 62-63 21.48635901969444 27.008620536844052 29.1788596391647 22.326160804296805 64-65 21.138114925479268 28.180506590459366 28.524152443027955 22.157226041033415 66-67 21.77443025806369 27.03736079493365 27.770670334786345 23.41753861221632 68-69 21.158605112810438 27.19204064349049 28.499834639146393 23.149519604552676 70-71 22.341452702664462 27.064734227335553 27.90896903742894 22.68484403257105 72-73 22.093263654458777 26.19128013050453 29.093564714279896 22.6218915007568 74-75 21.15300961863136 28.39654265001877 27.925344782378232 22.525102948971643 76-77 21.708089147049257 26.874072956496732 27.868193626561183 23.549644269892834 78-79 21.83189070818169 27.12448284405507 28.2428708749613 22.800755572801943 80-81 22.405197769345968 26.96912315927107 27.928223686914954 22.697455384468014 82-83 21.933411908915186 26.586148696431096 28.702635425015323 22.777803969638395 84-85 21.686553631061987 26.962589926972168 28.29496499476864 23.055891447197205 86-87 21.677357255509946 27.05030457475985 28.18705931141079 23.085278858319413 88-89 21.711158603375388 27.016074279041273 27.491558514057076 23.781208603526263 90-91 22.045113269817055 26.120404788486866 27.938052294453424 23.896429647242652 92-93 22.555473626012322 26.799769792445368 27.93322698212119 22.711529599421123 94-95 22.264822448298546 26.34157227658446 28.02487019811306 23.368735077003933 96-97 21.228214624908162 26.257230353668394 28.884028569623133 23.630526451800314 98-99 22.016181561259778 26.77391747067986 27.438519329233525 23.771381638826846 100-101 22.172013971137382 25.381169894160337 28.239500901485375 24.20731523321691 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4780.0 1 3016.0 2 1471.0 3 1659.5 4 1407.0 5 1459.5 6 2279.5 7 3997.5 8 6028.0 9 6789.5 10 5963.5 11 5215.5 12 5320.0 13 5541.5 14 5873.0 15 6317.5 16 6713.0 17 6749.5 18 6740.0 19 7081.0 20 7623.5 21 8385.0 22 9445.0 23 10894.0 24 12403.5 25 14546.0 26 17694.0 27 20858.0 28 22981.0 29 25900.5 30 29054.0 31 32482.0 32 36560.0 33 40085.0 34 45503.5 35 50031.0 36 54117.5 37 59006.0 38 61556.5 39 62495.5 40 63729.0 41 66983.5 42 70264.5 43 73744.5 44 78382.5 45 81804.5 46 88342.5 47 113539.0 48 164098.0 49 165570.5 50 119867.5 51 97056.0 52 87511.0 53 84297.0 54 82410.0 55 77472.0 56 70167.5 57 62607.0 58 57331.5 59 51438.5 60 43950.0 61 35753.5 62 28483.5 63 22898.5 64 17706.0 65 13216.0 66 9439.0 67 7300.5 68 5310.5 69 3171.5 70 2123.5 71 1493.5 72 1030.0 73 678.0 74 444.5 75 320.5 76 239.0 77 147.0 78 91.5 79 70.0 80 47.5 81 39.0 82 29.5 83 21.0 84 22.0 85 16.5 86 9.5 87 10.0 88 9.0 89 8.0 90 4.0 91 4.5 92 6.0 93 7.0 94 6.5 95 2.5 96 1.5 97 1.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3252557363574435 2 0.08632450884169399 3 0.0960453991851717 4 0.10111227253022766 5 0.11278484742143062 6 0.056261060327695345 7 0.0582127448754206 8 0.05900092517354041 9 0.062116113970871106 10-11 0.05984540406438307 12-13 0.05817521248027204 14-15 0.056261060327695345 16-17 0.0574057983797265 18-19 0.06618837884449015 20-21 0.06787733662617547 22-23 0.08324685243951185 24-25 0.09270501601694962 26-27 0.08791963563550789 28-29 0.08529236797510852 30-31 0.07913705517074425 32-33 0.07103005781865472 34-35 0.3206580179517446 36-37 0.49993150337885384 38-39 0.4727955816864431 40-41 0.45493016159572736 42-43 0.4856879594199744 44-45 0.49426411171142093 46-47 0.4895725623178506 48-49 0.5065184387274266 50-51 0.503140523164056 52-53 0.5016392273581135 54-55 0.49334456803028115 56-57 0.47270175069857173 58-59 0.45630009401864985 60-61 0.4155211466897366 62-63 0.48439309178734896 64-65 0.5076444105818835 66-67 0.5171588727520441 68-69 0.529225537792307 70-71 0.5268046983052247 72-73 0.5152822529946159 74-75 0.5171964051471927 76-77 0.4927065173127556 78-79 0.4729832436621859 80-81 0.47913855646655024 82-83 0.48754581297982824 84-85 0.4896851595032963 86-87 0.4896288609105734 88-89 0.49441424129201517 90-91 0.5085076556703004 92-93 0.5147005008698133 94-95 0.521081008045069 96-97 0.5122233627900081 98-99 0.5162768614660529 100-101 0.49486463003379794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2664365.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.4312184938911 #Duplication Level Percentage of deduplicated Percentage of total 1 66.43041064091204 24.86571215338887 2 14.949007083454429 11.19119100815017 3 6.4622058339532105 7.256647155696011 4 3.545438724913729 5.308403662757938 5 2.1214499957365396 3.9704229157139377 6 1.3742028858387374 3.086285308485928 7 0.9638340294844685 2.525423750463643 8 0.700417293450277 2.0973978198429726 9 0.5137187561583716 1.7306207105556575 >10 2.6755566015273975 17.684080535798692 >50 0.16767717972015075 4.3018048894150125 >100 0.08793941479968016 5.875985044469334 >500 0.003517958046765791 0.9312888822326633 >1k 0.0031159056985633395 2.830285975183052 >5k 9.046177834538727E-4 2.475649001728369 >10k+ 6.030785223025818E-4 3.8688011861176603 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 33601 1.261126009386852 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17174 0.6445813542814142 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14569 0.5468094649194085 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13595 0.5102529120447086 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12055 0.45245302351592215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11836 0.44423342897838697 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8971 0.3367031168777551 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7946 0.29823241185047844 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7885 0.2959429357464161 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7785 0.2921896962315598 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7538 0.28291919462986487 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7061 0.26501624214400055 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6852 0.25717197155795096 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6657 0.24985315450398124 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5106 0.19164040962856066 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4608 0.17294927684457648 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4311 0.16180215548545338 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4206 0.1578612539948543 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4052 0.15208126514197567 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4027 0.1511429552632616 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3848 0.14442465653166892 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3281 0.1231437884824339 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3214 0.1206291180074802 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3170 0.11897769262094345 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2932 0.11004498257558555 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2905 0.10903160790657436 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2857 0.10723005293944336 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2792 0.1047904472547868 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.7532395148562606E-5 0.0 0.0 0.0 0.0 3 3.7532395148562606E-5 0.0 0.0 0.0 0.0 4 3.7532395148562606E-5 0.0 0.0 0.0 0.0 5 7.506479029712521E-5 0.0 0.0 0.0 0.0 6 1.1259718544568781E-4 0.0 0.0 0.0 0.0 7 1.87661975742813E-4 0.0 0.0 0.0 0.0 8 1.87661975742813E-4 0.0 0.0 0.0 0.0 9 1.87661975742813E-4 0.0 0.0 3.7532395148562606E-5 0.0 10-11 2.2519437089137562E-4 0.0 0.0 9.383098787140652E-5 0.0 12-13 2.627267660399382E-4 0.0 0.0 1.1259718544568781E-4 0.0 14-15 3.3779155633706343E-4 0.0 0.0 1.1259718544568781E-4 0.0 16-17 3.3779155633706343E-4 0.0 0.0 2.4396056846565691E-4 0.0 18-19 5.254535320798764E-4 0.0 0.0 3.002591611885008E-4 0.0 20-21 6.380507175255643E-4 0.0 0.0 5.066873345055951E-4 0.0 22-23 6.755831126741269E-4 0.0 0.0 9.570760762883464E-4 0.0 24-25 7.50647902971252E-4 0.0 0.0 0.0019141521525766927 0.0 26-27 7.50647902971252E-4 0.0 0.0 0.0032653183779249462 0.0 28-29 0.0010133746690111902 0.0 0.0 0.010602901629468935 0.0 30-31 0.001125971854456878 0.0 0.0 0.02741741465602498 0.0 32-33 0.001125971854456878 0.0 0.0 0.06256650271265386 0.0 34-35 0.0012198028423282847 0.0 0.0 0.11567484184786994 0.0 36-37 0.0012761014350511286 0.0 0.0 0.1931041730393546 0.0 38-39 0.0013886986204968163 0.0 0.0 0.31026154449559273 0.0 40-41 0.001426231015645379 0.0 0.0 0.4391290232381825 0.0 42-43 0.0015763605962396295 0.0 0.0 0.5686157865007235 0.0 44-45 0.001801554967131005 0.0 0.0 0.7319567701872678 0.0 46-47 0.0018766197574281301 0.0 0.0 0.9268624981937534 0.0 48-49 0.0018766197574281301 0.0 0.0 1.1227816008692502 0.0 50-51 0.0018766197574281301 0.0 0.0 1.3414641012023503 0.0 52-53 0.0018953859550024114 0.0 0.0 1.5547419366340574 0.0 54-55 0.0019141521525766927 0.0 0.0 1.7897322626592076 0.0 56-57 0.0021018141283195057 0.0 0.0 2.0923934971372167 0.0 58-59 0.0021018141283195057 0.0 0.0 2.4052823092932085 0.0 60-61 0.0021018141283195057 0.0 0.0 2.762459347724505 0.0 62-63 0.0021018141283195057 0.0 0.0 3.1161458734069845 0.0 64-65 0.0021018141283195057 0.0 0.0 3.4692694131622357 0.0 66-67 0.0024020732895080068 0.0 0.0 3.8810748527322643 0.0 68-69 0.002477138079805132 0.0 0.0 4.3605887331503 0.0 70-71 0.002570969067676538 0.0 0.0 4.874857611475905 0.0 72-73 0.0026460338579736634 0.0 0.0 5.381582478376648 0.0 74-75 0.002664800055547945 0.0 0.0 5.930512523621951 0.0 76-77 0.002664800055547945 0.0 0.0 6.485109960534686 0.0 78-79 0.0027023324506965075 0.0 0.0 7.168725005770606 0.0 80-81 0.003115188797330696 0.0 0.0 7.901676384429311 0.0 82-83 0.003115188797330696 0.0 0.0 8.595950629887422 0.0 84-85 0.003190253587627821 0.0 0.0 9.32856421698979 0.0 86-87 0.0032277859827763837 0.0 0.0 10.087150221534962 0.0 88-89 0.0032277859827763837 0.0 0.0 10.900815015960651 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 880 0.0 32.28985 3 CTTTGCG 985 0.0 28.847782 2 GTACATG 12060 0.0 26.135021 1 TACATGG 12045 0.0 25.674606 2 ACATGGG 12035 0.0 24.633472 3 GTATAGG 630 0.0 24.110792 1 GAGTACT 11710 0.0 22.758142 12-13 CATGGGA 7630 0.0 21.724056 4 GTACTTT 12655 0.0 21.003382 14-15 AGTACTT 11770 0.0 20.993235 12-13 TTGCGTT 1405 0.0 20.898388 4 CATGGGG 4720 0.0 19.665792 4 CTACACG 455 0.0 18.735209 4 ATGGGAG 3465 0.0 18.314663 5 AGTACAT 8825 0.0 17.923761 2 ACTTTTT 15260 0.0 17.731144 16-17 ATGGGGA 2625 0.0 17.139244 5 ATGGGAT 2975 0.0 16.873932 5 AGAGTAC 22860 0.0 16.719963 10-11 CTATGCG 170 0.0018308824 16.705957 9 >>END_MODULE