##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939043_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2664365 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13651470425411 33.0 31.0 34.0 30.0 34.0 2 32.36207013678681 34.0 31.0 34.0 30.0 34.0 3 32.449948111463705 34.0 31.0 34.0 30.0 34.0 4 36.00025559561096 37.0 35.0 37.0 35.0 37.0 5 35.9332670261019 37.0 35.0 37.0 35.0 37.0 6 35.99113522358986 37.0 35.0 37.0 35.0 37.0 7 35.92477945026301 37.0 35.0 37.0 35.0 37.0 8 35.90785796991028 37.0 35.0 37.0 35.0 37.0 9 37.56042659320326 39.0 37.0 39.0 35.0 39.0 10-11 37.46847485235694 39.0 37.0 39.0 34.0 39.0 12-13 37.391409022412475 39.0 37.0 39.0 34.0 39.0 14-15 38.63672132008941 40.0 38.0 41.0 34.0 41.0 16-17 38.75422736749657 40.0 38.0 41.0 34.5 41.0 18-19 38.81716994480861 40.0 38.0 41.0 34.0 41.0 20-21 38.789984480354605 40.0 38.0 41.0 34.0 41.0 22-23 38.708758372069894 40.0 38.0 41.0 34.0 41.0 24-25 38.607640282018416 40.0 38.0 41.0 34.0 41.0 26-27 38.42573258543781 40.0 38.0 41.0 34.0 41.0 28-29 38.26715896658303 40.0 38.0 41.0 34.0 41.0 30-31 38.070485838089 40.0 37.5 41.0 33.0 41.0 32-33 37.93213692568398 40.0 37.0 41.0 33.0 41.0 34-35 37.79058556166291 40.0 37.0 41.0 33.0 41.0 36-37 37.6310002570969 40.0 37.0 41.0 33.0 41.0 38-39 37.462715506321395 40.0 37.0 41.0 32.5 41.0 40-41 37.307843895262096 40.0 36.0 41.0 32.0 41.0 42-43 37.08373233397076 40.0 36.0 41.0 31.0 41.0 44-45 36.79146776061088 40.0 36.0 41.0 31.0 41.0 46-47 36.40690277045375 39.0 35.5 41.0 30.0 41.0 48-49 35.99943175953745 39.0 35.0 41.0 29.0 41.0 50-51 35.70691008927081 39.0 35.0 40.0 28.0 41.0 52-53 35.46227506366432 39.0 35.0 40.0 27.5 41.0 54-55 35.166722652489426 38.0 34.5 40.0 27.0 41.0 56-57 35.007017619582896 38.0 34.0 40.0 26.5 41.0 58-59 34.72805884328911 38.0 34.0 40.0 26.0 41.0 60-61 34.31754827135171 37.5 34.0 40.0 26.0 41.0 62-63 33.91723712779593 37.0 33.0 40.0 25.0 41.0 64-65 33.497255818928714 36.0 33.0 39.0 23.5 41.0 66-67 33.014836555802226 36.0 32.5 39.0 22.0 40.0 68-69 32.501778284882135 35.0 32.0 38.5 21.5 40.0 70-71 31.940660344960243 35.0 31.0 37.5 19.5 39.5 72-73 31.4161241046178 35.0 31.0 37.0 18.0 39.0 74-75 30.914644952924995 34.5 30.5 36.5 15.5 39.0 76-77 29.028452370452246 32.5 28.0 34.5 10.5 36.5 78-79 29.993525661836873 34.0 29.5 35.0 9.0 37.0 80-81 29.972864265969562 34.0 30.0 35.0 7.0 37.0 82-83 29.566607615698302 34.0 29.5 35.0 4.5 36.5 84-85 29.119627378380965 34.0 29.0 35.0 2.0 36.0 86-87 28.47460670741434 33.5 28.5 35.0 2.0 35.5 88-89 27.779464337656442 33.0 26.5 35.0 2.0 35.0 90-91 27.19174005813768 33.0 25.5 35.0 2.0 35.0 92-93 26.805302389124613 32.0 25.0 34.0 2.0 35.0 94-95 26.245503900554166 32.0 25.0 34.0 2.0 35.0 96-97 25.366340760368793 32.0 21.5 34.0 2.0 35.0 98-99 24.171354525374714 31.5 10.0 34.0 2.0 35.0 100-101 18.712809243478276 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1234473400518965 1101 2 0.10412909701076956 1101 3 0.2018270329315257 1101 4 0.10820533089603401 1101 5 0.1562912420373479 1101 6 0.16515043295007814 1101 7 0.195054017085603 1101 8 0.2214633916459121 1101 9 0.22466595406014278 1101 10-11 0.28904087522441557 1101 12-13 0.2587663788863708 1101 14-15 0.21283431837394318 1101 16-17 0.22501878585170232 1101 18-19 0.2043367260322242 1101 20-21 0.33117547529464275 1101 22-23 0.2663584689059988 1101 24-25 0.22989408346379037 1101 26-27 0.16543090765079427 1101 28-29 0.27855062687973486 1101 30-31 0.22730858679775423 1101 32-33 0.34727647900093217 1101 34-35 0.4457751493765869 1101 36-37 0.47817124012777157 1101 38-39 0.41393794595997946 1101 40-41 0.40666218972616264 1101 42-43 0.3739212251452315 1101 44-45 0.375105579141092 1101 46-47 0.40038782653127925 1101 48-49 0.22416152917882926 1101 50-51 0.0719237761740743 1101 52-53 0.0639726163882699 1101 54-55 0.17551764995135244 1101 56-57 -0.0403516164031501 1101 58-59 0.06756125062963036 1101 60-61 0.07074129336648127 1101 62-63 0.14629648652883276 1101 64-65 0.13726578485903929 1101 66-67 0.16532781669554453 1101 68-69 0.25271322737100377 1101 70-71 0.3378920187269294 1101 72-73 0.34816626009436646 1101 74-75 0.22269568263167727 1101 76-77 -0.17929182570381386 1101 78-79 0.221560507501966 1101 80-81 0.22836326577508714 1101 82-83 0.17542560370719684 1101 84-85 0.2769738947941889 1101 86-87 0.47047330940224796 1101 88-89 0.5200769480630001 1101 90-91 0.5183783802091959 1101 92-93 0.47706251784943987 1101 94-95 0.4377821166142617 1101 96-97 0.5582660891126245 1101 98-99 1.005346963838516 1101 100-101 1.5217194951828859 1102 1 0.07686694611716405 1102 2 0.08660941781472786 1102 3 0.06472705964719694 1102 4 0.053190610290698714 1102 5 -0.016435024865714354 1102 6 0.09242915836964016 1102 7 0.10804660416115297 1102 8 0.07347779382202901 1102 9 0.136708313242913 1102 10-11 0.152979844755059 1102 12-13 0.1538835837793897 1102 14-15 0.2964649984901939 1102 16-17 0.2929486113362998 1102 18-19 0.1342133144678499 1102 20-21 0.17824680344135402 1102 22-23 0.15619882252435247 1102 24-25 0.27151082690428296 1102 26-27 0.25111501776253675 1102 28-29 0.32771437910940904 1102 30-31 0.25586382887142634 1102 32-33 0.28270348449789395 1102 34-35 0.30088563487321096 1102 36-37 0.24530823080966968 1102 38-39 0.36035902408578124 1102 40-41 0.369388429643692 1102 42-43 0.43519102240296803 1102 44-45 0.3450705181942766 1102 46-47 0.3027236600737595 1102 48-49 0.25494965500694633 1102 50-51 0.07247478691701303 1102 52-53 0.14623091376338948 1102 54-55 0.08344100565568624 1102 56-57 0.0032178672827498644 1102 58-59 -0.07378928058047762 1102 60-61 0.027692967683542236 1102 62-63 0.03158792348120443 1102 64-65 0.08692563228287042 1102 66-67 -0.09870195913568125 1102 68-69 -0.05111851516547716 1102 70-71 0.08544173281526568 1102 72-73 0.21837093182891465 1102 74-75 0.15171919107410403 1102 76-77 0.12377335918753118 1102 78-79 0.2809645928409985 1102 80-81 0.3104088834603189 1102 82-83 0.2831801649360095 1102 84-85 0.3944730987211251 1102 86-87 0.35504929255248285 1102 88-89 0.35271000695351873 1102 90-91 0.5771747851971973 1102 92-93 0.7401474628934146 1102 94-95 0.835130181347143 1102 96-97 0.8095986682004614 1102 98-99 1.1092517022273185 1102 100-101 1.2533099682122 1103 1 0.1274712185787905 1103 2 0.1812622212039443 1103 3 0.12855663130677897 1103 4 0.10917159725553915 1103 5 0.18630156070442894 1103 6 0.16126495107702965 1103 7 0.15101877898813143 1103 8 0.174982699736276 1103 9 0.14706710086133512 1103 10-11 0.08062655948793207 1103 12-13 0.1525499593199342 1103 14-15 0.2489051386360117 1103 16-17 0.19616986111170576 1103 18-19 0.2543323080495625 1103 20-21 0.18011126025133706 1103 22-23 0.24814165372929864 1103 24-25 0.13004734060476864 1103 26-27 0.1878901576179075 1103 28-29 0.26759202170379126 1103 30-31 0.18370820203161742 1103 32-33 0.23080130287571876 1103 34-35 0.18432989671650546 1103 36-37 0.1347060556719697 1103 38-39 0.27281812458036114 1103 40-41 0.3169242597719375 1103 42-43 0.4203815006198397 1103 44-45 0.40729560326930425 1103 46-47 0.38731310806890207 1103 48-49 0.21976848134312377 1103 50-51 0.19633316385171895 1103 52-53 0.2754708179011658 1103 54-55 0.3921089989388449 1103 56-57 0.33644549008596414 1103 58-59 0.2517628458560708 1103 60-61 0.3119676651082628 1103 62-63 0.34721077465292893 1103 64-65 0.3296694318367912 1103 66-67 0.2081770607351956 1103 68-69 0.16895881901083953 1103 70-71 0.08265779703323162 1103 72-73 0.0704084431936387 1103 74-75 0.07187253213477263 1103 76-77 -0.04598162541858386 1103 78-79 0.23291924133491193 1103 80-81 0.18240888278485556 1103 82-83 0.23369210110711336 1103 84-85 0.21241009862250593 1103 86-87 0.2700888445019487 1103 88-89 0.188338913683733 1103 90-91 0.2069797171844634 1103 92-93 0.22429307864205583 1103 94-95 0.48841872448717183 1103 96-97 0.14850890746425804 1103 98-99 0.5695023476357015 1103 100-101 0.877636696832667 1104 1 0.1589653432532927 1104 2 0.11765213935072438 1104 3 0.06881887762378369 1104 4 0.07880713774148518 1104 5 0.08542369520824877 1104 6 0.1351453192365426 1104 7 0.1146768274440646 1104 8 0.12720759676975035 1104 9 0.13668278137376433 1104 10-11 0.14274089333548545 1104 12-13 0.1318482369222309 1104 14-15 0.22434217138663826 1104 16-17 0.25043670542257956 1104 18-19 0.21290186329015626 1104 20-21 0.1536508788651787 1104 22-23 0.16762567775059978 1104 24-25 0.31495867738965444 1104 26-27 0.1238077430611284 1104 28-29 0.1194462192315342 1104 30-31 0.05781311989173332 1104 32-33 0.1878015268754183 1104 34-35 0.26494012256954136 1104 36-37 0.22353693723079715 1104 38-39 0.1876970714084294 1104 40-41 0.2942568733284503 1104 42-43 0.15180877538347204 1104 44-45 0.19162264083325198 1104 46-47 0.28942357192161694 1104 48-49 0.19217485146796065 1104 50-51 0.1301049188227097 1104 52-53 0.19095401392357303 1104 54-55 0.2955194610582268 1104 56-57 0.16911454652722568 1104 58-59 0.20931681911743993 1104 60-61 0.2320251883648936 1104 62-63 0.30077041990673337 1104 64-65 0.3388915862072466 1104 66-67 0.2554055810971718 1104 68-69 0.3398630911384757 1104 70-71 0.3887025556402186 1104 72-73 0.258742192081975 1104 74-75 0.24741196474885996 1104 76-77 0.1240783978347153 1104 78-79 0.3355678544700922 1104 80-81 0.4018385068786898 1104 82-83 0.3025360093152365 1104 84-85 0.40126726347962105 1104 86-87 0.4701194111278575 1104 88-89 0.7120367987532177 1104 90-91 0.6118188440656276 1104 92-93 0.7654253571323508 1104 94-95 0.6748135592940621 1104 96-97 0.5534300342360865 1104 98-99 0.7523181872145805 1104 100-101 1.0651427801578919 1105 1 -0.009126751484423323 1105 2 0.004170221046322808 1105 3 0.05133057265075536 1105 4 0.072488463455187 1105 5 0.004856318413999361 1105 6 -0.0029267131703605287 1105 7 0.046950622264340325 1105 8 0.07499066121878428 1105 9 0.09379834935850795 1105 10-11 0.14921915434417343 1105 12-13 0.15564713685375153 1105 14-15 0.1258034898049445 1105 16-17 0.030429969982961325 1105 18-19 0.03573404365274513 1105 20-21 0.07583007355618321 1105 22-23 0.018076120273782692 1105 24-25 -0.0023084459856832495 1105 26-27 -0.006334229126785829 1105 28-29 0.12176154659629645 1105 30-31 0.17486042155623238 1105 32-33 0.12256615445840424 1105 34-35 0.0822996382578367 1105 36-37 0.09912200855286812 1105 38-39 0.16990607161961435 1105 40-41 0.04225439993423663 1105 42-43 0.12438057740954633 1105 44-45 0.13615565568642296 1105 46-47 0.16289105942665572 1105 48-49 0.18355764841926003 1105 50-51 0.23979787236898176 1105 52-53 0.23302035400408272 1105 54-55 0.11918053245708649 1105 56-57 0.0961882939914318 1105 58-59 0.010332014653172905 1105 60-61 0.027547240930125838 1105 62-63 0.14334599761867395 1105 64-65 -0.006502237026658975 1105 66-67 -0.052732381824320385 1105 68-69 0.029804248034224656 1105 70-71 0.16607579818643714 1105 72-73 0.17333617786235678 1105 74-75 0.20470415803837838 1105 76-77 -0.16763565155637394 1105 78-79 -0.03727240522029973 1105 80-81 -0.0035438453096077183 1105 82-83 0.02694357860547214 1105 84-85 0.004309330379655307 1105 86-87 0.23345766138543667 1105 88-89 0.3601117589165348 1105 90-91 0.2337610184767165 1105 92-93 0.3770701482857248 1105 94-95 0.3641975625449909 1105 96-97 0.3453836636941183 1105 98-99 0.6316169495575537 1105 100-101 0.8843171848474185 1106 1 -0.060666981020723654 1106 2 -0.010519040204819419 1106 3 -0.04489869057261586 1106 4 -0.026662722532933003 1106 5 -0.09240760281848992 1106 6 9.138099235954655E-4 1106 7 0.037813924522531295 1106 8 0.025522023341501665 1106 9 -0.009097782810712829 1106 10-11 -0.006520390982799995 1106 12-13 0.02241974724129392 1106 14-15 7.460298751880146E-4 1106 16-17 -0.12017800537942946 1106 18-19 -0.06156363291801625 1106 20-21 -0.061915600829962614 1106 22-23 -0.02295557940232129 1106 24-25 0.027549857448271098 1106 26-27 0.009384001031527589 1106 28-29 0.010230116204354545 1106 30-31 0.08261959807769159 1106 32-33 0.1327378894827902 1106 34-35 0.007982140855858688 1106 36-37 -0.04186683322183171 1106 38-39 0.047827804747633706 1106 40-41 0.23563076816326145 1106 42-43 0.15650124705476998 1106 44-45 0.19026735930695793 1106 46-47 0.24775994459023565 1106 48-49 0.35259641775272854 1106 50-51 0.2183552678222469 1106 52-53 0.1785961290493958 1106 54-55 0.15587751571595732 1106 56-57 0.15531020006815055 1106 58-59 0.2607211492269812 1106 60-61 0.24750992042128672 1106 62-63 0.31154240058913985 1106 64-65 0.37528846470510757 1106 66-67 0.4131371784460285 1106 68-69 0.24899776094090242 1106 70-71 0.23645795412852166 1106 72-73 0.3311750018112001 1106 74-75 0.30479063238811577 1106 76-77 0.04001808694215825 1106 78-79 0.3551626062716835 1106 80-81 0.2711411680787421 1106 82-83 0.2209612982616953 1106 84-85 0.2827688732792737 1106 86-87 0.4544053140619724 1106 88-89 0.389354501518806 1106 90-91 0.6376265736192828 1106 92-93 0.701099397855252 1106 94-95 0.8167971028655501 1106 96-97 0.604507845411618 1106 98-99 0.5327149462351173 1106 100-101 0.8301076286732254 1107 1 -0.01205156111817729 1107 2 -0.022745748471130867 1107 3 -0.08150294796894997 1107 4 -0.11176242347421805 1107 5 -0.04854977480224676 1107 6 -0.058941747951763546 1107 7 -0.02936136740523665 1107 8 -0.05609189391597624 1107 9 -0.16473240775226827 1107 10-11 -0.0725850194236628 1107 12-13 -0.02542905902335235 1107 14-15 -0.01651101064917526 1107 16-17 -0.06413757805187004 1107 18-19 -0.01780813767737044 1107 20-21 0.010723320323812402 1107 22-23 -0.048865914718597026 1107 24-25 -0.05379381894491786 1107 26-27 -0.017957081755106685 1107 28-29 -0.08252616417568959 1107 30-31 -0.033946402151677546 1107 32-33 -0.007852494176901814 1107 34-35 -0.011709566954657191 1107 36-37 0.033626966529396896 1107 38-39 -0.0322374470997957 1107 40-41 0.04310003157311826 1107 42-43 0.10482481270506838 1107 44-45 -0.1269560113817576 1107 46-47 -0.0950454437597088 1107 48-49 0.0590560271826277 1107 50-51 -0.10721866319723006 1107 52-53 -0.09935806821172832 1107 54-55 -0.11102459475051063 1107 56-57 -0.04622361532705099 1107 58-59 -0.10523761772187612 1107 60-61 -0.04729433873940536 1107 62-63 -0.0606785147010811 1107 64-65 0.007590775720288434 1107 66-67 -0.04272328434512218 1107 68-69 -0.09925890612764476 1107 70-71 -0.05482229358737456 1107 72-73 0.030093450234598862 1107 74-75 -0.052377660918789815 1107 76-77 -0.0070180853415386935 1107 78-79 0.2897390279768217 1107 80-81 0.1579114221757294 1107 82-83 0.04606414564177186 1107 84-85 0.2233795470582649 1107 86-87 0.14216213845245917 1107 88-89 0.41084658984257416 1107 90-91 0.5015527999217504 1107 92-93 0.535304863248836 1107 94-95 0.5530471653532381 1107 96-97 0.569578993156032 1107 98-99 0.47930120717781577 1107 100-101 0.5469124053789933 1108 1 0.12564731531321627 1108 2 0.14208770631154977 1108 3 0.1821753663639214 1108 4 0.11963974046894066 1108 5 0.09444374474012562 1108 6 0.14638566403985465 1108 7 0.16286417825647703 1108 8 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9 1816.0 10 3148.0 11 4645.0 12 7025.0 13 9995.0 14 11670.0 15 15523.0 16 20080.0 17 28583.0 18 28256.0 19 18623.0 20 16842.0 21 18307.0 22 19476.0 23 21411.0 24 24541.0 25 28751.0 26 33873.0 27 40830.0 28 50053.0 29 60333.0 30 72448.0 31 87792.0 32 108222.0 33 138059.0 34 187088.0 35 269360.0 36 410602.0 37 577844.0 38 332735.0 39 14712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.260319623072064 19.45949999171024 14.431382626769299 21.8487977584484 2 15.697436349749378 22.40751548680455 37.816440315046925 24.07860784839915 3 20.929452233459003 24.914491820752787 30.851478682537863 23.30457726325034 4 12.781919894609034 16.857487619001148 37.77688117056034 32.583711315829476 5 12.726146755418272 38.72416129171491 34.234573716439 14.315118236427818 6 30.513649593805653 36.664420978356944 16.908794403169235 15.913135024668168 7 26.898229033934918 34.301268782618 21.018141283195057 17.78236090025203 8 25.65729545313799 33.16602642655943 20.054159246199376 21.12251887410321 9 25.438969510558803 17.563884828092245 21.794423812052777 35.202721849296175 10-11 25.14940708198764 25.9983335616554 27.126651190809064 21.72560816554789 12-13 27.12727047532902 24.06639105377829 27.383466604613105 21.422871866279582 14-15 23.029097659531185 25.487325557042688 27.716040532394913 23.767536251031213 16-17 21.130085429472548 28.68433132417747 28.06396269493372 22.121620551416264 18-19 20.74564791315268 28.153015702868313 30.011346089896463 21.089990294082543 20-21 22.52258981033004 26.037479849795353 29.24169548841844 22.198234851456164 22-23 21.67674098706446 26.261754677005587 29.748514186307055 22.312990149622895 24-25 22.757582723822303 26.228262858376066 29.60514944944616 21.409004968355468 26-27 22.16675349164003 25.47399679623355 31.13947643790413 21.21977327422229 28-29 20.97439727664941 27.78144135657089 30.159325017405646 21.084836349374054 30-31 21.621211808442162 26.319085410595022 29.690526635802527 22.369176145160292 32-33 21.664486660048453 26.625537416983036 30.076810046671532 21.633165876296978 34-35 22.699053962873602 26.21201329200462 29.580584889494492 21.50834785562728 36-37 22.24633414241016 25.6587600382996 30.661283749831767 21.433622069458472 38-39 21.711875655012328 26.67152013909534 30.33409006963776 21.282514136254576 40-41 21.75949298195897 26.985123141800276 29.726485011476218 21.528898864764535 42-43 21.28586192963554 27.293417212525128 28.68498328878323 22.73573756905611 44-45 21.986852155244407 26.271687094056073 28.917920741353782 22.82354000934574 46-47 22.680950406011256 27.278082523406404 28.63162853019609 21.40933854038625 48-49 22.44488000126113 26.901021590089325 28.66140470326295 21.9926937053866 50-51 20.890089682303316 27.517767470071025 29.658777874299147 21.933364973326512 52-53 22.038215484740267 28.275330144330823 27.62662022658307 22.05983414434584 54-55 21.194337112219984 26.996413779643554 28.440022294242716 23.369226813893743 56-57 22.208931584073504 27.024563076004977 28.08254499665023 22.683960343271288 58-59 23.215847341148635 26.69523778067864 27.88725638486893 22.201658493303796 60-61 21.955932186213733 27.091134322739467 29.429080643487442 21.523852847559354 62-63 21.40446994346419 27.17091245112747 29.02586354763244 22.398754057775896 64-65 21.057490082464874 28.29129019067152 28.380486547076366 22.27073317978724 66-67 21.614277250506653 27.179484099824553 27.65602676430853 23.55021188536027 68-69 21.019081624342085 27.315747416479656 28.32104300919081 23.344127949987445 70-71 22.184487555003745 27.24231749115361 27.732715950223017 22.840479003619627 72-73 21.856577458418798 26.319892357090712 28.895121351616616 22.928408832873874 74-75 20.864258463085953 28.485286362791882 27.660999900539153 22.98945527358301 76-77 21.359635257742067 26.91281472177471 27.777693252782893 23.949856767700332 78-79 21.412348818870154 27.164522678060905 28.165434372104038 23.257694130964907 80-81 21.905977244023415 26.995257764074232 27.824726810053463 23.27403818184889 82-83 21.381139855301328 26.712411158458238 28.609880517474238 23.29656846876619 84-85 21.08117207200157 26.98512606317806 28.18059206371815 23.75310980110222 86-87 20.962619197573776 27.08475422482578 28.056103980800284 23.89652259680016 88-89 20.95310703582939 26.983357521156492 27.389128672936653 24.674406770077464 90-91 21.13052222756408 26.14319699543196 27.83494317453954 24.89133760246442 92-93 21.506512808868155 26.8868567182049 27.791349908890112 23.815280564036833 94-95 21.139551577080606 26.364302570526444 27.94315660084355 24.552989251549402 96-97 19.920506387075346 26.389195924732533 28.8060382117315 24.88425947646062 98-99 20.655559579862366 26.836938632657315 27.30547428749439 25.202027499985924 100-101 20.505387615971017 25.405586576243145 28.39128553257435 25.69774027521149 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3006.0 1 2058.5 2 1254.0 3 1421.0 4 1300.0 5 1279.0 6 2026.0 7 3705.5 8 5503.0 9 6242.5 10 5439.5 11 4674.0 12 4748.5 13 4991.5 14 5311.5 15 5532.5 16 5710.0 17 5930.5 18 6265.5 19 6666.5 20 7222.0 21 8112.0 22 9336.5 23 10889.5 24 12340.5 25 14457.5 26 17761.5 27 20979.0 28 23242.5 29 26111.5 30 29375.0 31 32883.0 32 36913.5 33 40366.0 34 45468.0 35 49949.5 36 54162.5 37 58986.5 38 61635.0 39 62468.5 40 63781.0 41 67291.5 42 70530.5 43 74522.0 44 79447.5 45 82482.5 46 87515.0 47 114675.0 48 166678.5 49 165528.5 50 120824.5 51 100167.5 52 89929.5 53 85128.5 54 82715.0 55 77834.0 56 70367.0 57 62696.5 58 57411.0 59 51697.5 60 43866.5 61 35345.5 62 28276.0 63 22516.0 64 17243.0 65 12888.5 66 9081.5 67 6876.0 68 4884.5 69 2748.5 70 1751.5 71 1216.0 72 803.0 73 516.5 74 348.5 75 239.0 76 154.5 77 73.0 78 32.0 79 18.0 80 10.0 81 4.5 82 3.0 83 5.5 84 4.5 85 2.0 86 3.5 87 3.0 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3936772927132732 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010133746690111902 16-17 0.002327008499210881 18-19 4.128563466341886E-4 20-21 0.0 22-23 0.0 24-25 9.38309878714065E-5 26-27 3.7532395148562606E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01341783126561113 36-37 0.02362664274602016 38-39 0.010996991778528843 40-41 0.006005183223770016 42-43 0.005123171937778796 44-45 0.0018766197574281304 46-47 0.001125971854456878 48-49 0.0032653183779249462 50-51 5.817521248027203E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.131155078226894E-4 60-61 0.0014825296083682228 62-63 0.0013511662253482537 64-65 6.943493102484082E-4 66-67 0.0026460338579736634 68-69 0.0027773972409936326 70-71 3.7532395148562606E-5 72-73 0.0 74-75 0.0 76-77 0.09122248640858141 78-79 0.0035280451439648848 80-81 3.75323951485626E-4 82-83 1.8766197574281303E-5 84-85 2.4396056846565694E-4 86-87 0.09197313431155266 88-89 0.4321855301356984 90-91 0.3855702953611836 92-93 0.0 94-95 0.0010321408665854715 96-97 0.0 98-99 0.0 100-101 5.254535320798764E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2664365.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.876163423037355 #Duplication Level Percentage of deduplicated Percentage of total 1 64.79030580423466 23.244275992612884 2 15.300031068794876 10.978128300032676 3 6.891305648997335 7.417008229845868 4 3.7429053884113093 5.37124341566445 5 2.3151731897530157 4.152976585410693 6 1.4931388391729654 3.2140855804472164 7 0.9894953552879492 2.484950795085281 8 0.7383938945230547 2.1192592024385695 9 0.5550891641308294 1.7923022610043355 >10 2.900340407602106 18.28560108568193 >50 0.17979432707066909 4.4370817510078195 >100 0.0950253233108716 6.115379469064857 >500 0.003977446174315007 0.9966154757088594 >1k 0.003454097993483531 2.932730672094088 >5k 9.420267254955084E-4 2.584103012844655 >10k+ 6.280178169970057E-4 3.8742581710558563 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34384 1.2905138747881766 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17002 0.6381257823158614 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14226 0.5339358533834516 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13150 0.4935509962035982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12706 0.47688661275763644 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11704 0.4392791528187767 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8874 0.33306247454834453 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8472 0.3179744516986224 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8288 0.31106849099128686 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8054 0.3022859105265232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8012 0.30070954993028354 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7568 0.28404516648432176 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7402 0.2778147888896604 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6619 0.24842692348833587 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5526 0.20740401559095697 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4455 0.1672068203868464 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4393 0.1648798118876355 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4359 0.16360371045258437 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4309 0.16172709069515626 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4257 0.15977540614743102 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3827 0.14363647623354908 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3461 0.12989961960917518 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3311 0.12426976033689079 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3061 0.11488666154975012 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3000 0.1125971854456878 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2930 0.10996991778528843 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2898 0.10876888114053443 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2735 0.10265110073131872 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.7532395148562606E-5 0.0 0.0 0.0 0.0 6 3.7532395148562606E-5 0.0 0.0 0.0 0.0 7 7.506479029712521E-5 0.0 0.0 0.0 0.0 8 7.506479029712521E-5 0.0 0.0 0.0 0.0 9 7.506479029712521E-5 0.0 0.0 3.7532395148562606E-5 0.0 10-11 9.383098787140652E-5 0.0 0.0 9.383098787140652E-5 0.0 12-13 1.87661975742813E-4 0.0 0.0 1.1259718544568781E-4 0.0 14-15 2.627267660399382E-4 0.0 0.0 1.1259718544568781E-4 0.0 16-17 2.627267660399382E-4 0.0 0.0 2.4396056846565691E-4 0.0 18-19 3.9409014905990736E-4 0.0 0.0 3.002591611885008E-4 0.0 20-21 4.5038874178275124E-4 0.0 0.0 5.066873345055951E-4 0.0 22-23 4.8792113693131383E-4 0.0 0.0 9.007774835655025E-4 0.0 24-25 5.62985927228439E-4 0.0 0.0 0.0018578535598538488 0.0 26-27 5.62985927228439E-4 0.0 0.0 0.003227785982776384 0.0 28-29 6.755831126741268E-4 0.0 0.0 0.010715498814914622 0.0 30-31 8.257126932683773E-4 0.0 0.0 0.027830271002659168 0.0 32-33 8.257126932683773E-4 0.0 0.0 0.06318578723260514 0.0 34-35 8.632450884169399E-4 0.0 0.0 0.11670698271445541 0.0 36-37 8.632450884169399E-4 0.0 0.0 0.19492449420405988 0.0 38-39 9.758422738626277E-4 0.0 0.0 0.3132078375147549 0.0 40-41 9.758422738626277E-4 0.0 0.0 0.44370797544630713 0.0 42-43 0.0010321408665854715 0.0 0.0 0.5742081133778593 0.0 44-45 0.0012010366447540034 0.0 0.0 0.7390879252654947 0.0 46-47 0.0012573352374768473 0.0 0.0 0.9359641040172799 0.0 48-49 0.0012761014350511286 0.0 0.0 1.133365736301145 0.0 50-51 0.0012761014350511286 0.0 0.0 1.353774726811079 0.0 52-53 0.0012761014350511286 0.0 0.0 1.5689104158026397 0.0 54-55 0.0012761014350511286 0.0 0.0 1.8067532038590808 0.0 56-57 0.0013511662253482537 0.0 0.0 2.1131301454567977 0.0 58-59 0.0013511662253482537 0.0 0.0 2.4286837576683373 0.0 60-61 0.0013511662253482537 0.0 0.0 2.78908858208241 0.0 62-63 0.0013511662253482537 0.0 0.0 3.145195947251972 0.0 64-65 0.0013886986204968163 0.0 0.0 3.500871689877325 0.0 66-67 0.001501295805942504 0.0 0.0 3.9178378337802817 0.0 68-69 0.0016514253865367546 0.0 0.0 4.400729629761688 0.0 70-71 0.0017264901768338798 0.0 0.0 4.9190332405657635 0.0 72-73 0.0018766197574281301 0.0 0.0 5.4316131611096825 0.0 74-75 0.0019516845477252553 0.0 0.0 5.987205206493855 0.0 76-77 0.0019516845477252553 0.0 0.0 6.547620164654617 0.0 78-79 0.001989216942873818 0.0 0.0 7.235044747998116 0.0 80-81 0.0024020732895080068 0.0 0.0 7.973363259163065 0.0 82-83 0.0024396056846565694 0.0 0.0 8.673267363893462 0.0 84-85 0.002477138079805132 0.0 0.0 9.409108736978606 0.0 86-87 0.0025146704749536945 0.0 0.0 10.169571361281207 0.0 88-89 0.0025146704749536945 0.0 0.0 10.986219981121206 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12000 0.0 26.04455 1 TACATGG 12160 0.0 25.44447 2 CTTTGCG 910 0.0 25.03106 2 TTTGCGT 910 0.0 25.03106 3 ACATGGG 11955 0.0 24.809032 3 GTAAGCG 495 0.0 23.010288 94-95 CATGGGA 7540 0.0 22.846237 4 GAGTACT 11920 0.0 21.938951 12-13 GTACTTT 12800 0.0 20.505 14-15 AGTACTT 11990 0.0 20.30667 12-13 ATGGGAT 2990 0.0 19.362795 5 CATGGGG 4675 0.0 19.083395 4 ATGGGAG 3110 0.0 18.00533 5 TTGCGTT 1270 0.0 17.935642 4 GTATAGG 665 0.0 17.910704 1 ACTTTTT 15295 0.0 17.391544 16-17 GGACCGA 275 5.9729064E-7 17.256262 6 TACTTTT 14820 0.0 16.525175 14-15 TGGACCG 260 6.6898174E-6 16.426634 5 AGAGTAC 22375 0.0 16.046574 10-11 >>END_MODULE