##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939042_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2675930 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.851731547536744 33.0 31.0 34.0 30.0 34.0 2 31.889130507898187 33.0 31.0 34.0 30.0 34.0 3 31.93953690866353 34.0 31.0 34.0 30.0 34.0 4 35.474804647356244 37.0 35.0 37.0 33.0 37.0 5 35.52640950996476 37.0 35.0 37.0 33.0 37.0 6 35.62830567316783 37.0 35.0 37.0 33.0 37.0 7 35.5469253680029 37.0 35.0 37.0 33.0 37.0 8 35.543794493876895 37.0 35.0 37.0 33.0 37.0 9 37.14051526011517 39.0 37.0 39.0 33.0 39.0 10-11 37.03067662457538 39.0 37.0 39.0 33.0 39.0 12-13 36.92122196768974 39.0 37.0 39.0 33.0 39.0 14-15 38.09442156558654 40.0 38.0 41.0 33.0 41.0 16-17 38.048855724925545 40.0 38.0 41.0 33.0 41.0 18-19 38.02861696681154 40.0 38.0 41.0 33.0 41.0 20-21 37.90964431057614 40.0 38.0 41.0 33.0 41.0 22-23 37.97705769582912 40.0 38.0 41.0 33.0 41.0 24-25 37.89131871909953 40.0 38.0 41.0 32.5 41.0 26-27 37.7917428707029 40.0 37.5 41.0 32.5 41.0 28-29 37.67048689614452 40.0 37.0 41.0 32.0 41.0 30-31 37.49060588281458 40.0 37.0 41.0 32.0 41.0 32-33 37.27241239494307 40.0 36.5 41.0 31.5 41.0 34-35 36.91829905864503 40.0 36.0 41.0 30.5 41.0 36-37 36.628060898453995 39.0 35.5 41.0 30.0 41.0 38-39 36.39910236814865 39.0 35.0 41.0 30.0 41.0 40-41 36.26603965724066 39.0 35.0 41.0 30.0 41.0 42-43 36.03610613880034 39.0 35.0 41.0 29.5 41.0 44-45 35.91010657976853 39.0 35.0 41.0 28.5 41.0 46-47 35.61947024025292 39.0 35.0 41.0 27.0 41.0 48-49 35.37146412649061 39.0 35.0 41.0 26.0 41.0 50-51 34.15640562346549 38.0 34.0 40.0 23.5 40.5 52-53 34.31519415679782 38.0 33.5 39.5 24.0 40.5 54-55 34.82579495726719 39.0 35.0 40.0 24.0 41.0 56-57 34.7051873554241 38.5 34.5 40.5 23.5 41.0 58-59 34.502021166472964 38.0 34.0 40.0 23.0 41.0 60-61 34.18385234292377 38.0 34.0 40.0 23.0 41.0 62-63 33.843014764960216 37.5 34.0 40.0 22.0 41.0 64-65 33.42659972420803 37.0 33.0 39.5 21.5 41.0 66-67 33.00121079400432 36.0 33.0 39.0 20.0 41.0 68-69 32.58066503981793 36.0 32.5 39.0 18.5 40.5 70-71 32.1140255911029 35.0 32.0 38.5 16.5 40.0 72-73 31.6570752971864 35.0 31.5 37.0 12.5 40.0 74-75 31.19974009036111 35.0 31.0 37.0 8.0 39.0 76-77 30.792189257566527 35.0 31.0 36.5 7.0 39.0 78-79 30.345291917202616 35.0 30.5 36.0 2.0 38.5 80-81 29.884412522001696 34.0 30.0 35.5 2.0 37.0 82-83 29.495226893080165 34.0 29.5 35.0 2.0 37.0 84-85 29.12589062494161 34.0 29.0 35.0 2.0 36.0 86-87 28.76340674083403 34.0 29.0 35.0 2.0 36.0 88-89 28.321265317104707 34.0 29.0 35.0 2.0 36.0 90-91 27.86314272047475 34.0 27.5 35.0 2.0 35.0 92-93 27.419271430867028 34.0 26.5 35.0 2.0 35.0 94-95 26.908616443628944 33.5 25.5 35.0 2.0 35.0 96-97 26.281306872750783 33.0 24.5 35.0 2.0 35.0 98-99 25.590000672663336 33.0 22.0 35.0 2.0 35.0 100-101 23.038451117929093 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22896245293750894 1101 2 0.3302239208482405 1101 3 0.2734164163596269 1101 4 0.1862842798310922 1101 5 0.02498903080176973 1101 6 0.1320842321381619 1101 7 0.12620588183546744 1101 8 0.2508782093134698 1101 9 0.162587669990792 1101 10-11 0.2610661942352621 1101 12-13 0.37721838607770763 1101 14-15 0.132009586932881 1101 16-17 0.2975703733805517 1101 18-19 0.3329370501922 1101 20-21 0.4976372781472733 1101 22-23 0.438098975005623 1101 24-25 0.4662663838206811 1101 26-27 0.38748624032759693 1101 28-29 0.3827942323965843 1101 30-31 0.5443432574312439 1101 32-33 0.4061468602735232 1101 34-35 0.29089292821448964 1101 36-37 0.3159394145381995 1101 38-39 0.3894977900891661 1101 40-41 0.19578738680494467 1101 42-43 0.19302916958265826 1101 44-45 0.38809963167301476 1101 46-47 0.333907178253007 1101 48-49 0.43341186669139375 1101 50-51 0.3307914632625142 1101 52-53 0.27213518899946365 1101 54-55 0.18301673536795704 1101 56-57 0.19801204113858262 1101 58-59 0.19649602012467682 1101 60-61 0.07169595358754322 1101 62-63 0.04173701758041659 1101 64-65 0.12600205969618372 1101 66-67 0.1086175576867845 1101 68-69 -0.02566022246285371 1101 70-71 -0.11257108685258288 1101 72-73 -0.1729241601568674 1101 74-75 -0.13830533313374005 1101 76-77 -0.21671219157067867 1101 78-79 -0.21316353822710354 1101 80-81 -0.1997415866542127 1101 82-83 -0.3678367145188126 1101 84-85 -0.39392283425842933 1101 86-87 -0.3866336081227395 1101 88-89 -0.419381431319362 1101 90-91 -0.2912598353384759 1101 92-93 -0.3757935409361828 1101 94-95 -0.3937952494995969 1101 96-97 -0.5548136688976548 1101 98-99 -0.5373119707022376 1101 100-101 -0.18158167006397363 1102 1 0.1366393645988424 1102 2 0.11896419005597636 1102 3 0.15335861740625845 1102 4 0.14850179395538277 1102 5 0.2522537994757812 1102 6 0.21988364113084913 1102 7 0.2094936529307816 1102 8 0.1046488099539431 1102 9 0.2540662297033549 1102 10-11 0.1654863822120376 1102 12-13 0.19326150097266037 1102 14-15 0.31584508121807175 1102 16-17 0.2974873850184494 1102 18-19 0.3709971402752501 1102 20-21 0.33577628853546315 1102 22-23 0.3119665906686251 1102 24-25 0.3453289082290212 1102 26-27 0.32935907612128545 1102 28-29 0.46661264084252707 1102 30-31 0.30187046459622735 1102 32-33 0.21786286433506774 1102 34-35 0.1992850042904024 1102 36-37 0.310285303522555 1102 38-39 0.30905879792587143 1102 40-41 0.31141604805620915 1102 42-43 0.24107015607020088 1102 44-45 0.4031060429307871 1102 46-47 0.3242175547272623 1102 48-49 0.27611767782941143 1102 50-51 0.08929793310031187 1102 52-53 0.18548655196126873 1102 54-55 0.18690091615891902 1102 56-57 0.10999279434746256 1102 58-59 0.11678700010333642 1102 60-61 0.03965707598072754 1102 62-63 -0.07777029190433637 1102 64-65 -0.060361162465284224 1102 66-67 -0.09747736798167494 1102 68-69 -0.072608203404819 1102 70-71 -0.0604820582395007 1102 72-73 -0.20700731775013637 1102 74-75 -0.21894691453393378 1102 76-77 -0.18050957629821696 1102 78-79 -0.218493669185996 1102 80-81 -0.4124614244526441 1102 82-83 -0.22406832690132816 1102 84-85 -0.3025205912945417 1102 86-87 -0.3251706372044687 1102 88-89 -0.29897947474668385 1102 90-91 -0.2849237552530788 1102 92-93 -0.2708121238204022 1102 94-95 -0.3100300512387726 1102 96-97 -0.7953240310523562 1102 98-99 -0.5420785944102242 1102 100-101 -0.14756701855000642 1103 1 -0.0013956167689741505 1103 2 0.18843182057055685 1103 3 0.2543133099313657 1103 4 0.19870382930816533 1103 5 0.13621739350386974 1103 6 0.17484336067733608 1103 7 0.21745234590522244 1103 8 0.21068084601448334 1103 9 0.20721694879078 1103 10-11 0.3232304430609432 1103 12-13 0.3615448075865473 1103 14-15 0.31337199058689436 1103 16-17 0.3836994705180672 1103 18-19 0.36232614525357576 1103 20-21 0.35796495182957244 1103 22-23 0.5005157671059024 1103 24-25 0.5606144101876964 1103 26-27 0.4309767225116943 1103 28-29 0.35338713328673776 1103 30-31 0.3480611745412503 1103 32-33 0.39114413533645376 1103 34-35 0.3703431721612347 1103 36-37 0.44970154699609566 1103 38-39 0.5674879219242186 1103 40-41 0.5119243667961229 1103 42-43 0.32873444168708943 1103 44-45 0.4455985611620221 1103 46-47 0.49321235174065947 1103 48-49 0.33820872912099986 1103 50-51 0.2888618185424079 1103 52-53 0.23015850667522386 1103 54-55 0.2607017715706945 1103 56-57 0.4043012932082348 1103 58-59 0.27958405505577844 1103 60-61 0.16710102845843267 1103 62-63 0.1434736783646784 1103 64-65 0.07910913674702869 1103 66-67 0.17684349389730158 1103 68-69 0.1251890495896717 1103 70-71 0.0725737889681426 1103 72-73 0.11463585333806137 1103 74-75 0.0549728310372295 1103 76-77 -0.06390858816580547 1103 78-79 0.021198566981414757 1103 80-81 0.004143881645688907 1103 82-83 -0.13524387202017607 1103 84-85 -0.23965263079646348 1103 86-87 -0.18004299098937437 1103 88-89 -0.2468424712072519 1103 90-91 -0.14045630330100423 1103 92-93 -0.3049696691898838 1103 94-95 -0.42505449482179003 1103 96-97 -0.4285248385444511 1103 98-99 -0.5850050195175385 1103 100-101 -0.2995884759186964 1104 1 0.1516426698605713 1104 2 0.08972258897635754 1104 3 0.1767480078509429 1104 4 0.2071999258208166 1104 5 0.13852776342052664 1104 6 0.10207362177123258 1104 7 0.04940976656801155 1104 8 0.0976144859349759 1104 9 0.23945898185273506 1104 10-11 0.21457972324967756 1104 12-13 0.2995027056759767 1104 14-15 0.24900640700909804 1104 16-17 0.24255020412449113 1104 18-19 0.2650867806315276 1104 20-21 0.3365681641925704 1104 22-23 0.2436814459147385 1104 24-25 0.2926860381712544 1104 26-27 0.3198242012253658 1104 28-29 0.2954171437793178 1104 30-31 0.412029273413701 1104 32-33 0.2984729840802487 1104 34-35 0.29044374008280727 1104 36-37 0.30854218852782367 1104 38-39 0.3750210914745651 1104 40-41 0.3175932043448668 1104 42-43 0.407998788246708 1104 44-45 0.42583565657710665 1104 46-47 0.5428276132461818 1104 48-49 0.6675140002354496 1104 50-51 0.6727451090228271 1104 52-53 0.5894962027515191 1104 54-55 0.6094539143485918 1104 56-57 0.688469641092702 1104 58-59 0.6748576424593509 1104 60-61 0.5151983783590879 1104 62-63 0.3758134217812312 1104 64-65 0.2566245982822366 1104 66-67 0.27058210058070387 1104 68-69 0.20541285843084012 1104 70-71 0.22288777168859752 1104 72-73 0.29109426938137517 1104 74-75 0.14425527022333995 1104 76-77 0.1507879836756203 1104 78-79 0.12466958905906367 1104 80-81 -0.060941179445535454 1104 82-83 -0.2398559342585571 1104 84-85 -0.06702292538650667 1104 86-87 -0.04863821869360052 1104 88-89 -0.017101397289717823 1104 90-91 -0.17514784976602726 1104 92-93 -0.06728732447195185 1104 94-95 -0.027255981583053313 1104 96-97 -0.06356148628463743 1104 98-99 -0.07931189741742273 1104 100-101 0.19135321159697938 1105 1 0.0763012884755021 1105 2 0.016652690057711084 1105 3 0.12803361143092573 1105 4 0.06998989824700885 1105 5 0.1255637416371087 1105 6 0.09578777385465287 1105 7 0.09594270738012511 1105 8 0.0607856388630168 1105 9 0.0965008010242343 1105 10-11 0.06691524965167162 1105 12-13 0.10734877762994444 1105 14-15 0.17235655306379982 1105 16-17 0.21525834614432426 1105 18-19 0.14419905414422374 1105 20-21 0.14991920897430333 1105 22-23 0.1580857124478925 1105 24-25 0.12646816978219988 1105 26-27 0.09442076451170323 1105 28-29 0.2244315053755841 1105 30-31 0.24437236268843776 1105 32-33 0.18358005977511027 1105 34-35 0.18844816527287378 1105 36-37 0.24393466015510512 1105 38-39 0.0974980386637938 1105 40-41 0.037904188561959984 1105 42-43 0.05036786352750511 1105 44-45 -0.14524578951939304 1105 46-47 -0.011995352173791218 1105 48-49 -0.0024396744713044427 1105 50-51 -0.009544018091240503 1105 52-53 -0.0014949760037481497 1105 54-55 -0.0741769829309149 1105 56-57 -0.17961757951765378 1105 58-59 -0.2633024613675232 1105 60-61 -0.09421038412153848 1105 62-63 -0.2757529607715625 1105 64-65 -0.2062122682712939 1105 66-67 -0.285965403309028 1105 68-69 -0.22552775421117843 1105 70-71 -0.4831053964444152 1105 72-73 -0.25027114516687377 1105 74-75 -0.2618175038510202 1105 76-77 -0.33209196011308606 1105 78-79 -0.3173833285433929 1105 80-81 -0.4645796827613431 1105 82-83 -0.4418553167113046 1105 84-85 -0.3716959359663612 1105 86-87 -0.22994907449909263 1105 88-89 -0.22586836384551034 1105 90-91 -0.1452721130372403 1105 92-93 -0.11515132163671993 1105 94-95 -0.06855595708773166 1105 96-97 -0.3653426923454788 1105 98-99 -0.4188237606912857 1105 100-101 -0.054080584516544405 1106 1 0.12264938110691048 1106 2 0.23898579136967157 1106 3 0.23871110282908958 1106 4 0.07718550965430637 1106 5 0.13187300342828934 1106 6 0.10995392294672257 1106 7 0.15210407429287898 1106 8 0.13471508417399747 1106 9 0.20343466113904185 1106 10-11 0.2292265423545743 1106 12-13 0.2360019520691239 1106 14-15 0.09734177557189128 1106 16-17 0.18517389176591337 1106 18-19 0.34193509140119716 1106 20-21 0.30819971770989696 1106 22-23 0.231303798838411 1106 24-25 0.338928541962062 1106 26-27 0.3949366196883801 1106 28-29 0.3626854761049074 1106 30-31 0.3355432648696919 1106 32-33 0.48325380034442844 1106 34-35 0.4007202012798672 1106 36-37 0.27622539182614503 1106 38-39 0.28995788902050634 1106 40-41 0.2802619535154136 1106 42-43 0.4609848054458894 1106 44-45 0.4811668442390271 1106 46-47 0.42466971203594284 1106 48-49 0.42775275831322546 1106 50-51 0.3064559645146474 1106 52-53 0.26104167532512434 1106 54-55 0.22582034797364514 1106 56-57 0.250399937545815 1106 58-59 0.44155983579997127 1106 60-61 0.4227461693024992 1106 62-63 0.3870428927136871 1106 64-65 0.1122787451242715 1106 66-67 0.3209145500747752 1106 68-69 0.3031495020666668 1106 70-71 0.43405712717632383 1106 72-73 0.44849856677775435 1106 74-75 0.4097808164782215 1106 76-77 0.33504994549510414 1106 78-79 0.4550501187613172 1106 80-81 0.4210521266184557 1106 82-83 0.384568383296763 1106 84-85 0.38675347552808503 1106 86-87 0.5108991520369379 1106 88-89 0.672771162219103 1106 90-91 0.4646013508458502 1106 92-93 0.6338986003447182 1106 94-95 0.582653697960172 1106 96-97 0.7419062859757695 1106 98-99 0.6392880702092221 1106 100-101 1.0758792470965233 1107 1 -0.030753694319642477 1107 2 0.025663140822725694 1107 3 0.0886828035778997 1107 4 0.06488597474634616 1107 5 -0.02837574047579494 1107 6 -9.127000044770739E-4 1107 7 -0.01781331505415551 1107 8 0.06789874269540519 1107 9 0.04551094318196647 1107 10-11 0.12258888422979197 1107 12-13 0.10328302370603382 1107 14-15 0.055500717483880635 1107 16-17 0.08871687116319293 1107 18-19 0.1950353431270173 1107 20-21 0.09086103429817882 1107 22-23 0.10039812147303451 1107 24-25 0.21938056278915496 1107 26-27 0.24442498949228053 1107 28-29 0.25297260855843007 1107 30-31 0.3383784011041655 1107 32-33 0.3205263025917304 1107 34-35 0.14348731798425263 1107 36-37 0.12966921527934971 1107 38-39 0.245311151974839 1107 40-41 0.2521209175746364 1107 42-43 0.1906800565275617 1107 44-45 0.3351749156776265 1107 46-47 0.42805129161185107 1107 48-49 0.3684636557686076 1107 50-51 0.4185823022151922 1107 52-53 0.32772491623353517 1107 54-55 0.37211748866362626 1107 56-57 0.5146874405675348 1107 58-59 0.37668745746141497 1107 60-61 0.3509161558838798 1107 62-63 0.2757002488543421 1107 64-65 0.30197405410721245 1107 66-67 0.42557886015929824 1107 68-69 0.31925351303316774 1107 70-71 0.2656949662426129 1107 72-73 0.1754112994142396 1107 74-75 0.20636920061269137 1107 76-77 0.3238218737959464 1107 78-79 0.30753538650407464 1107 80-81 0.2709660708392576 1107 82-83 0.3230933626332444 1107 84-85 0.34382054402964357 1107 86-87 0.465924331856602 1107 88-89 0.47707648436012207 1107 90-91 0.4821599143729607 1107 92-93 0.32963178255570824 1107 94-95 0.37451393584758463 1107 96-97 0.4649261707232668 1107 98-99 0.4287984569753043 1107 100-101 0.840460961003398 1108 1 0.12653173743347423 1108 2 0.09762628137661977 1108 3 0.005345799708937449 1108 4 0.06381708569755062 1108 5 0.07072566879183739 1108 6 0.1402027503339056 1108 7 0.14782538358469566 1108 8 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-2.203208672278688 2316 74-75 -2.2073921894751543 2316 76-77 -2.160727669430827 2316 78-79 -1.979938733578738 2316 80-81 -1.8451420575017359 2316 82-83 -2.0035459912168676 2316 84-85 -2.132450934839479 2316 86-87 -2.185527867790146 2316 88-89 -2.3452317118242725 2316 90-91 -2.6953897692194637 2316 92-93 -2.6601917076362405 2316 94-95 -2.737260987828588 2316 96-97 -3.031574338102221 2316 98-99 -3.4468378673161446 2316 100-101 -4.168436759224463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7872.0 3 1844.0 4 1810.0 5 2102.0 6 2415.0 7 3134.0 8 3780.0 9 4735.0 10 6336.0 11 8273.0 12 11669.0 13 17831.0 14 17128.0 15 16535.0 16 21320.0 17 29044.0 18 24919.0 19 16529.0 20 16584.0 21 17782.0 22 19157.0 23 21107.0 24 24434.0 25 28136.0 26 32213.0 27 37753.0 28 45063.0 29 53096.0 30 63799.0 31 77458.0 32 96084.0 33 122908.0 34 165202.0 35 237300.0 36 357693.0 37 533457.0 38 459387.0 39 70031.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.16823455529028 18-19 20.78130272109098 28.129000256614944 29.992245440595884 21.09745158169819 20-21 22.57075918124271 25.985963754600256 29.166526386510363 22.276750677646675 22-23 21.752829141126327 26.15144771813045 29.722368547074034 22.37335459366919 24-25 22.76886390568412 26.158284724968766 29.494554079604097 21.57829728974302 26-27 22.228090676790593 25.38871183742747 31.088976872052598 21.29422061372934 28-29 21.07351033543246 27.657023854912204 30.105410298727993 21.164055510927344 30-31 21.622036700772743 26.298142476307916 29.625519078344702 22.45430174457464 32-33 21.631805901785555 26.606313377080053 29.98797061845536 21.773910102679032 34-35 22.761259606163474 26.155735901566562 29.546817345552544 21.536187146717413 36-37 22.289825867519035 25.594867911648784 30.597951141565645 21.517355079266544 38-39 21.701297803220378 26.630559785688835 30.276732145622148 21.391410265468632 40-41 21.762123557345518 26.97745100985814 29.675421525607113 21.58500390718923 42-43 21.31812469843026 27.238474211183743 28.609498563738878 22.833902526647115 44-45 21.96152337616428 26.252974771457303 28.882143703520224 22.903358148858192 46-47 22.707207029451613 27.191789702740337 28.60309192142979 21.497911346378256 48-49 22.406771741481897 26.837476362489504 28.701994544459758 22.05375735156884 50-51 20.885916675922726 27.507522779772138 29.67058390603437 21.93597663827077 52-53 21.981150958662354 28.21529894120975 27.736921586371704 22.066628513756193 54-55 21.207568841605813 26.85388754532076 28.574201825628137 23.364341787445287 56-57 22.260711944633417 26.920060052985058 28.170305936930085 22.64892206545144 58-59 23.250569847108327 26.582238053970013 27.955638626099706 22.21155347282196 60-61 22.03007929627583 26.92085187306963 29.531858866861832 21.51720996379271 62-63 21.474336268884926 26.998719035448914 29.189587451245085 22.337357244421078 64-65 21.143497315317024 28.163597898118326 28.54273359279473 22.150171193769918 66-67 21.745617026492415 27.065474738002916 27.776221609697753 23.412686625806913 68-69 21.16923980462663 27.19719374024651 28.48278827225863 23.150778182868226 70-71 22.328972803823547 27.11774745599225 27.879159185488906 22.6741205546953 72-73 22.087169634483935 26.19346630944211 29.09058680205259 22.62877725402136 74-75 21.171333738593596 28.390605480637543 27.91527053002253 22.52279025074633 76-77 21.71878382929587 26.82345542952681 27.899696889509006 23.558063851668308 78-79 21.79805424711103 27.141518507989304 28.267249499394314 22.793177745505353 80-81 22.429145857238225 26.972269731903854 27.903520033699674 22.69506437715825 82-83 21.942888110847367 26.629089165262332 28.70486528172603 22.723157442164272 84-85 21.70996017164175 26.949269537798976 28.280276778242325 23.06049351231695 86-87 21.647796385497568 27.05417844982634 28.220201533137136 23.07782363153896 88-89 21.726099807382536 27.00262304805438 27.504661327603984 23.766615816959096 90-91 22.027638561209926 26.08041955938896 28.008637943193627 23.883303936207483 92-93 22.553303635508513 26.81357177310601 27.974553959298515 22.65857063208696 94-95 22.231201168754318 26.352875982264166 28.01145595779732 23.404466891184192 96-97 21.23646783304314 26.258357833605167 28.920740547619374 23.584433785732315 98-99 22.0217763539166 26.802720474633762 27.40582506094676 23.76967811050288 100-101 22.07734069839819 25.54948565827021 28.20095411067443 24.172219532657166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5442.0 1 3362.5 2 1480.5 3 1658.0 4 1400.5 5 1381.5 6 2247.0 7 3966.0 8 5925.0 9 6845.5 10 6092.0 11 5243.5 12 5403.0 13 5719.5 14 6028.5 15 6299.0 16 6797.5 17 7174.5 18 7146.5 19 7260.0 20 7741.5 21 8563.5 22 9681.0 23 11148.5 24 12511.5 25 14528.5 26 17724.0 27 20837.5 28 22973.5 29 25839.0 30 29166.0 31 32686.0 32 36586.0 33 40193.0 34 45365.0 35 49788.5 36 54290.5 37 59368.5 38 61869.5 39 62461.0 40 63870.5 41 67580.5 42 70488.0 43 73935.5 44 78645.0 45 81509.0 46 88380.0 47 114377.5 48 165435.0 49 167186.5 50 120634.0 51 96931.0 52 87615.0 53 84781.5 54 82774.0 55 77896.0 56 70620.0 57 62839.0 58 57493.5 59 51801.5 60 44232.5 61 35763.0 62 28644.5 63 22973.0 64 17579.5 65 13265.5 66 9427.0 67 7133.5 68 5134.5 69 3044.0 70 2029.0 71 1409.0 72 977.5 73 637.0 74 439.5 75 313.0 76 216.0 77 131.0 78 69.5 79 41.0 80 32.5 81 29.5 82 27.5 83 23.0 84 16.0 85 13.5 86 10.5 87 8.5 88 10.0 89 7.5 90 6.0 91 3.5 92 3.5 93 4.5 94 2.5 95 2.0 96 1.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2834902258280299 2 0.08673620012481642 3 0.09017425717414133 4 0.10456177852185969 5 0.1060939561199284 6 0.08284970085166653 7 0.09219224718135377 8 0.08879156031734761 9 0.09342546329687249 10-11 0.08757702929448828 12-13 0.09138878819700068 14-15 0.09964759915244419 16-17 0.08882893050266637 18-19 0.09955417368914732 20-21 0.10119846184317227 22-23 0.11338114225708446 24-25 0.12627385619205286 26-27 0.1288897691643653 28-29 0.12074306876487802 30-31 0.11373615901761258 32-33 0.10805589084916271 34-35 0.3785039220009492 36-37 0.5818724705055812 38-39 0.5652614231313974 40-41 0.5295355259666732 42-43 0.5550967327246975 44-45 0.5766780147462751 46-47 0.5652240529460786 48-49 0.5866558542263811 50-51 0.5796302593864563 52-53 0.5823209127294062 54-55 0.5721935925080253 56-57 0.5461092031555385 58-59 0.5407652666549573 60-61 0.4883722668380713 62-63 0.5622157530279193 64-65 0.5954004775909684 66-67 0.6077513238388149 68-69 0.6093021865295429 70-71 0.6053783170710744 72-73 0.5971195061156309 74-75 0.5945970186066153 76-77 0.5797610550350719 78-79 0.5569465568979757 80-81 0.5522005433624945 82-83 0.5649624616488473 84-85 0.575071096777569 86-87 0.5671486174899941 88-89 0.5783223029003001 90-91 0.5906731491481466 92-93 0.5989319601035902 94-95 0.5980724458412588 96-97 0.5951388862937371 98-99 0.5989132750109307 100-101 0.577070401692122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2675930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.48262523538135 #Duplication Level Percentage of deduplicated Percentage of total 1 66.6584609498234 24.985341105495326 2 14.749303727888044 11.056852482304812 3 6.493510528886028 7.301814648487137 4 3.427842649094606 5.139381655274797 5 2.1183640744727965 3.970092335777965 6 1.3962331130222925 3.140068950998667 7 0.9762586105117165 2.561491494143736 8 0.7038867424787493 2.1106818381187473 9 0.5292986343045645 1.7855552112513435 >10 2.6859489245794363 17.718416352399004 >50 0.16571871298128654 4.281024835089813 >100 0.08697481834432515 5.822897352046761 >500 0.0035993474397825412 0.9424421950351483 >1k 0.0030994380731455013 2.8390565201713027 >5k 8.998368599454681E-4 2.4851347733162563 >10k+ 5.998912399636454E-4 3.8597482500891402 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 33350 1.2462956803802792 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17405 0.6504280754728263 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14646 0.5473237341783977 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13484 0.5038995788380115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12088 0.4517308001330379 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12020 0.44918962753136293 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8849 0.33068876988560986 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8054 0.3009794725572044 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7912 0.2956729062419421 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7776 0.29059056103859215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7741 0.289282604552436 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7064 0.2639829890916429 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7012 0.2620397394550679 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6689 0.24996916959711202 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5216 0.19492288662259477 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4652 0.1738461021028203 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4389 0.16401774336398933 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4222 0.15777692241575825 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4083 0.15258246665645214 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4077 0.15235824554453967 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3973 0.14847174627138976 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3380 0.1263112263773716 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3185 0.11902404024021557 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3029 0.1131942913304907 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3000 0.11211055595624697 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2915 0.10893409020415333 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2837 0.10601921574929091 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2687 0.10041368795147855 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.7370185318749E-5 0.0 0.0 0.0 0.0 3 3.7370185318749E-5 0.0 0.0 0.0 0.0 4 3.7370185318749E-5 0.0 0.0 0.0 0.0 5 7.4740370637498E-5 0.0 0.0 0.0 0.0 6 7.4740370637498E-5 0.0 0.0 0.0 0.0 7 1.1211055595624699E-4 0.0 0.0 0.0 0.0 8 1.8685092659374497E-4 0.0 0.0 0.0 0.0 9 1.8685092659374497E-4 0.0 0.0 0.0 0.0 10-11 1.8685092659374497E-4 0.0 0.0 5.6055277978123495E-5 0.0 12-13 1.8685092659374497E-4 0.0 0.0 1.1211055595624699E-4 0.0 14-15 2.6159129723124296E-4 0.0 0.0 1.1211055595624699E-4 0.0 16-17 2.6159129723124296E-4 0.0 0.0 1.86850926593745E-4 0.0 18-19 2.802763898906175E-4 0.0 0.0 2.98961482549992E-4 0.0 20-21 2.98961482549992E-4 0.0 0.0 4.2975713116561343E-4 0.0 22-23 3.36331667868741E-4 0.0 0.0 7.660887990343544E-4 0.0 24-25 3.36331667868741E-4 0.0 0.0 0.0018124539879593262 0.0 26-27 3.5501676052811546E-4 0.0 0.0 0.003195150844753039 0.0 28-29 4.1107203850623895E-4 0.0 0.0 0.010575762445205965 0.0 30-31 4.858124091437369E-4 0.0 0.0 0.02978403769904295 0.0 32-33 4.858124091437369E-4 0.0 0.0 0.06575284106833885 0.0 34-35 5.60552779781235E-4 0.0 0.0 0.11997697996584365 0.0 36-37 5.97922965099984E-4 0.0 0.0 0.19998654673328525 0.0 38-39 7.474037063749799E-4 0.0 0.0 0.3253261482923694 0.0 40-41 7.474037063749799E-4 0.0 0.0 0.45914878191880953 0.0 42-43 8.595142623312269E-4 0.0 0.0 0.5953257372203309 0.0 44-45 0.0010837353742437208 0.0 0.0 0.7621649295758858 0.0 46-47 0.0011584757448812187 0.0 0.0 0.9667293240107178 0.0 48-49 0.0012332161155187169 0.0 0.0 1.1689767669557873 0.0 50-51 0.0012332161155187169 0.0 0.0 1.3907688168225625 0.0 52-53 0.0012332161155187169 0.0 0.0 1.6040217793440037 0.0 54-55 0.0012332161155187169 0.0 0.0 1.847824868363522 0.0 56-57 0.001345326671474964 0.0 0.0 2.162294977820795 0.0 58-59 0.001345326671474964 0.0 0.0 2.4845567709170266 0.0 60-61 0.001345326671474964 0.0 0.0 2.8436655667375454 0.0 62-63 0.001345326671474964 0.0 0.0 3.201970903573711 0.0 64-65 0.001345326671474964 0.0 0.0 3.5633779657913323 0.0 66-67 0.0014948074127499598 0.0 0.0 3.9815503395081335 0.0 68-69 0.001569547783387458 0.0 0.0 4.4722769280212855 0.0 70-71 0.0016256030613655812 0.0 0.0 4.991554338117963 0.0 72-73 0.0017003434320030791 0.0 0.0 5.5047590931003425 0.0 74-75 0.0017937688952999519 0.0 0.0 6.055408773772109 0.0 76-77 0.0018311390806187008 0.0 0.0 6.603180950174332 0.0 78-79 0.0018311390806187008 0.0 0.0 7.295538373574795 0.0 80-81 0.0020553601925311945 0.0 0.0 8.03679468446484 0.0 82-83 0.0020553601925311945 0.0 0.0 8.743875960880889 0.0 84-85 0.0021114154705093183 0.0 0.0 9.47808799183835 0.0 86-87 0.0021674707484874416 1.86850926593745E-5 0.0 10.245447377173544 0.0 88-89 0.0021674707484874416 3.7370185318749E-5 0.0 11.072131931702248 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 915 0.0 26.90139 3 CTTTGCG 950 0.0 25.910284 2 GTACATG 11905 0.0 23.299284 1 GAGTACT 11490 0.0 22.761913 12-13 TACATGG 12085 0.0 22.718195 2 AGTACTT 11595 0.0 21.678055 12-13 ACATGGG 12175 0.0 21.306103 3 GTACTTT 12470 0.0 20.93591 14-15 CATGGGG 4480 0.0 18.807648 4 CATGGGA 7630 0.0 18.301638 4 TGCACCG 520 0.0 18.206533 5 AGTACAT 9240 0.0 17.93034 2 ACTTTTT 14945 0.0 17.707493 16-17 TACTTTT 14340 0.0 17.496029 14-15 TTGCGTT 1415 0.0 17.395597 4 AGAGTAC 22655 0.0 16.694569 10-11 GTATAGG 615 0.0 16.1905 1 ATGGGAG 3265 0.0 15.803159 5 TATAGCG 185 0.003217615 15.352535 5 GCGTAGA 530 7.2759576E-12 15.208596 1 >>END_MODULE