##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939042_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2675930 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11199881910214 33.0 31.0 34.0 30.0 34.0 2 32.3272290381288 34.0 31.0 34.0 30.0 34.0 3 32.420944120361895 34.0 31.0 34.0 30.0 34.0 4 35.99641059370013 37.0 35.0 37.0 35.0 37.0 5 35.91952181110866 37.0 35.0 37.0 35.0 37.0 6 35.978879866065256 37.0 35.0 37.0 35.0 37.0 7 35.9189108085787 37.0 35.0 37.0 35.0 37.0 8 35.898711849712065 37.0 35.0 37.0 35.0 37.0 9 37.55000766088799 39.0 37.0 39.0 35.0 39.0 10-11 37.472303834554715 39.0 37.0 39.0 34.0 39.0 12-13 37.407097719297596 39.0 37.0 39.0 34.0 39.0 14-15 38.60606667588465 40.0 38.0 41.0 34.0 41.0 16-17 38.620929732840544 40.0 38.0 41.0 34.0 41.0 18-19 38.747605318524776 40.0 38.0 41.0 34.0 41.0 20-21 38.74448640285807 40.0 38.0 41.0 34.0 41.0 22-23 38.67829633062151 40.0 38.0 41.0 34.0 41.0 24-25 38.59417155904676 40.0 38.0 41.0 34.0 41.0 26-27 38.41208439682653 40.0 38.0 41.0 34.0 41.0 28-29 38.23641705874219 40.0 38.0 41.0 33.5 41.0 30-31 38.06609552566771 40.0 37.5 41.0 33.0 41.0 32-33 37.87235914242899 40.0 37.0 41.0 33.0 41.0 34-35 37.79406169070192 40.0 37.0 41.0 33.0 41.0 36-37 37.663707010273065 40.0 37.0 41.0 33.0 41.0 38-39 37.51289140597848 40.0 37.0 41.0 33.0 41.0 40-41 37.37486500020554 40.0 36.5 41.0 32.0 41.0 42-43 37.16785510084344 40.0 36.0 41.0 31.5 41.0 44-45 36.874680578340985 40.0 36.0 41.0 31.0 41.0 46-47 36.5040042153569 39.5 36.0 41.0 30.0 41.0 48-49 36.152045270242496 39.0 35.0 41.0 29.5 41.0 50-51 35.85112241351605 39.0 35.0 41.0 28.0 41.0 52-53 35.64305718012056 39.0 35.0 40.0 28.0 41.0 54-55 35.39958556464481 38.5 35.0 40.0 27.5 41.0 56-57 35.08964995347412 38.0 34.5 40.0 26.5 41.0 58-59 34.78428434226605 38.0 34.0 40.0 26.0 41.0 60-61 34.31622445280706 37.5 34.0 40.0 26.0 41.0 62-63 34.024959359923464 37.0 33.5 40.0 25.5 41.0 64-65 33.77226422215828 36.5 33.0 39.5 25.0 41.0 66-67 33.28797857193574 36.0 33.0 39.0 23.0 40.0 68-69 32.84732242622191 35.5 32.5 39.0 22.0 40.0 70-71 32.35445004166776 35.0 32.0 37.5 21.5 40.0 72-73 31.83322097364281 35.0 31.0 37.0 20.0 39.0 74-75 31.39162422036451 35.0 31.0 37.0 18.5 39.0 76-77 29.423546206365636 32.5 28.5 35.0 13.5 37.0 78-79 30.40542297444253 34.0 30.0 35.0 14.5 37.0 80-81 30.397544778824557 34.0 31.0 35.0 13.0 37.0 82-83 30.082716476141005 34.0 30.0 35.0 9.0 37.0 84-85 29.697482931167855 34.0 30.0 35.0 6.5 36.0 86-87 29.17361795712145 34.0 29.0 35.0 2.0 36.0 88-89 28.60921249808478 34.0 29.0 35.0 2.0 35.0 90-91 28.14404599522409 33.5 28.0 35.0 2.0 35.0 92-93 27.802925151255828 33.0 27.0 35.0 2.0 35.0 94-95 27.408420437006946 33.0 27.0 35.0 2.0 35.0 96-97 26.776393627635997 33.0 25.5 35.0 2.0 35.0 98-99 25.911541968586622 32.5 24.5 34.0 2.0 35.0 100-101 21.691627023128408 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11838130676424896 1101 2 0.12923061105195188 1101 3 0.1506049520715962 1101 4 -0.02986546827067116 1101 5 0.1284061116882924 1101 6 0.13200429703523042 1101 7 0.08227629192698771 1101 8 0.182467143745626 1101 9 0.2825059744088918 1101 10-11 0.1966250989167122 1101 12-13 0.21038352781697967 1101 14-15 0.2473424664079218 1101 16-17 0.18449505425680712 1101 18-19 0.24198635452988526 1101 20-21 0.21833727938710012 1101 22-23 0.25077540519151853 1101 24-25 0.1447730030733183 1101 26-27 0.2750559605507803 1101 28-29 0.22553560867657296 1101 30-31 0.15794026424796925 1101 32-33 0.20457753939993495 1101 34-35 0.26782658519253033 1101 36-37 0.18902421020738558 1101 38-39 0.295966079569034 1101 40-41 0.4084950959498528 1101 42-43 0.2941712443455131 1101 44-45 0.40945018566187485 1101 46-47 0.2069448395847644 1101 48-49 -0.027820219066647667 1101 50-51 0.11540087734172744 1101 52-53 0.10983004615872716 1101 54-55 0.25274958799683134 1101 56-57 -0.05726535218265383 1101 58-59 -0.0438719030128496 1101 60-61 0.1596595260254503 1101 62-63 0.08381787553533826 1101 64-65 0.007572257288735784 1101 66-67 0.050979899286105024 1101 68-69 0.18000322065045538 1101 70-71 0.10049132581071518 1101 72-73 0.07622985265821924 1101 74-75 0.23654396129444422 1101 76-77 -0.01115569080080192 1101 78-79 0.2354616440265005 1101 80-81 0.4513059178038077 1101 82-83 0.32100827392102715 1101 84-85 0.22265114398332386 1101 86-87 0.5677353189688894 1101 88-89 0.7102018931663316 1101 90-91 0.7101839443688753 1101 92-93 0.6205056523875889 1101 94-95 0.5593354263602563 1101 96-97 0.4608213315960228 1101 98-99 0.41317108004007963 1101 100-101 0.80455050307582 1102 1 0.04070159627972458 1102 2 0.06222418026749921 1102 3 0.06270172626514636 1102 4 0.04235718787762721 1102 5 0.06429678835261399 1102 6 0.11071761943362901 1102 7 0.12506997309546364 1102 8 0.1212090401761472 1102 9 0.09030785678020692 1102 10-11 0.23371145888442868 1102 12-13 0.20525370798308984 1102 14-15 0.22989603458204044 1102 16-17 0.24651290814521332 1102 18-19 0.1566481200015204 1102 20-21 0.17353283902156136 1102 22-23 0.1490466437033291 1102 24-25 0.20471354435270683 1102 26-27 0.12631998721119686 1102 28-29 0.13675286895411176 1102 30-31 0.12163334399490111 1102 32-33 0.10216306622957916 1102 34-35 0.2654812689905981 1102 36-37 0.14232551979628028 1102 38-39 0.17236174855285924 1102 40-41 0.12889705252252526 1102 42-43 0.08472798234962653 1102 44-45 0.06950528342542128 1102 46-47 0.19966334306051436 1102 48-49 0.03969260344891268 1102 50-51 0.18460291983176802 1102 52-53 0.13104447506862016 1102 54-55 -0.005178404140423254 1102 56-57 0.12498783066511976 1102 58-59 0.025959711973229105 1102 60-61 -0.09640008890854546 1102 62-63 0.025808196814203654 1102 64-65 0.0429257877112903 1102 66-67 0.042590264044363835 1102 68-69 0.015389458251014787 1102 70-71 -0.12451697302550002 1102 72-73 -0.006565520821951054 1102 74-75 -0.06015586817260754 1102 76-77 -0.18373015251550484 1102 78-79 -0.14718060778947972 1102 80-81 0.011323752165548484 1102 82-83 -0.03478301197550593 1102 84-85 -0.03909772997851135 1102 86-87 0.1607721072216428 1102 88-89 0.21331216680011877 1102 90-91 0.3499239792565305 1102 92-93 0.372897181716656 1102 94-95 0.2734247348556593 1102 96-97 0.2576620785563719 1102 98-99 0.13785181892940201 1102 100-101 0.5053598836897386 1103 1 0.022834632485057682 1103 2 0.075781619278672 1103 3 0.10172022988542295 1103 4 0.03332711990050541 1103 5 0.06428250335257957 1103 6 -0.01592604838297973 1103 7 0.046424230057937166 1103 8 0.10700163366335858 1103 9 0.11629551843608965 1103 10-11 0.162956555071176 1103 12-13 0.12113778390916252 1103 14-15 0.15188662336429104 1103 16-17 0.1452590422670781 1103 18-19 0.15707261176394383 1103 20-21 0.16768410548421286 1103 22-23 0.11853550692251247 1103 24-25 0.08459682291604764 1103 26-27 0.05108300230719465 1103 28-29 0.16862124306786797 1103 30-31 0.12974462674245046 1103 32-33 0.07137126166396968 1103 34-35 0.10146901505783745 1103 36-37 0.08470323857267914 1103 38-39 0.16176438881046096 1103 40-41 0.12471869001208091 1103 42-43 0.15881372611438138 1103 44-45 0.24519049267144766 1103 46-47 0.21061766547426686 1103 48-49 0.154645459702202 1103 50-51 0.12065108495598054 1103 52-53 0.25676968231474717 1103 54-55 0.2565455226502493 1103 56-57 0.12621406805454427 1103 58-59 0.10611674308270835 1103 60-61 0.03357230857547222 1103 62-63 0.11549283595886806 1103 64-65 0.1538642342954546 1103 66-67 0.19674359533883745 1103 68-69 0.18150712639357636 1103 70-71 0.1176722162163415 1103 72-73 0.2262704023169526 1103 74-75 0.377198238148047 1103 76-77 0.17787027395668176 1103 78-79 0.3241834863434754 1103 80-81 0.34725419590310125 1103 82-83 0.46881697036304004 1103 84-85 0.3828849046559917 1103 86-87 0.287713000436721 1103 88-89 0.4591472776419785 1103 90-91 0.5346700990933932 1103 92-93 0.4528553591324602 1103 94-95 0.46780689886434246 1103 96-97 0.19343834169126595 1103 98-99 0.3048538584378164 1103 100-101 0.43917764085382416 1104 1 0.027114307873077337 1104 2 0.08441407799731593 1104 3 0.1103113667234652 1104 4 0.028934445062070324 1104 5 0.0835637696330025 1104 6 0.015285162688229548 1104 7 -0.04182087099471943 1104 8 -0.010405907834140748 1104 9 0.1014735187940019 1104 10-11 0.021911546960964756 1104 12-13 0.09645550943842096 1104 14-15 0.05687331612943325 1104 16-17 -0.004499876345654741 1104 18-19 0.08639081980562935 1104 20-21 0.03822972595291674 1104 22-23 0.07540547618220472 1104 24-25 -0.011522083020409468 1104 26-27 -0.01574711358797032 1104 28-29 -0.004574015850259627 1104 30-31 0.019634449894340378 1104 32-33 -0.12001031316376043 1104 34-35 -0.060423835398587755 1104 36-37 -0.1382798007024988 1104 38-39 0.025098466997178548 1104 40-41 -0.019292037148119334 1104 42-43 0.0442740820610652 1104 44-45 0.08379962359919091 1104 46-47 -0.016539757858545556 1104 48-49 -0.1791705714710261 1104 50-51 -0.16790922258052632 1104 52-53 -0.12091612072672575 1104 54-55 -0.055048952042163535 1104 56-57 -0.1436526974879655 1104 58-59 -0.07166325795351014 1104 60-61 0.01560619061040569 1104 62-63 0.09747792638862762 1104 64-65 -0.053255176085805545 1104 66-67 -0.08991987894766851 1104 68-69 -0.02435117197773451 1104 70-71 -0.10997908305483861 1104 72-73 -0.18324985700645158 1104 74-75 -0.07976756608858793 1104 76-77 -0.32484290483069955 1104 78-79 -0.21592861673999408 1104 80-81 -0.003244432051531021 1104 82-83 0.10502469609255272 1104 84-85 -0.009510216437778496 1104 86-87 -0.10508238664281677 1104 88-89 0.03812165585097915 1104 90-91 0.2280491895225154 1104 92-93 0.18960152485926685 1104 94-95 0.09515553125313048 1104 96-97 0.10342506147456376 1104 98-99 0.39742583718057034 1104 100-101 0.9042368121975493 1105 1 -0.058033680712625824 1105 2 -0.08955974299383485 1105 3 -0.07444224564277135 1105 4 -0.03090381798685371 1105 5 0.002893229858784707 1105 6 0.02882757620656662 1105 7 -0.03273494112903563 1105 8 0.01648961615069311 1105 9 -0.07173599883314807 1105 10-11 -0.06111973136030713 1105 12-13 -0.10739435659267116 1105 14-15 -0.014405988653571455 1105 16-17 0.010585557447370775 1105 18-19 -0.09582947684211973 1105 20-21 -0.04363921929765979 1105 22-23 -0.017862657562560003 1105 24-25 0.0038812966556847073 1105 26-27 -0.05656273226131958 1105 28-29 7.739753286983841E-5 1105 30-31 -0.04808987306591206 1105 32-33 0.0666521306677339 1105 34-35 0.16704050725966368 1105 36-37 0.16760525812250648 1105 38-39 0.2013888609617993 1105 40-41 0.17157507719662846 1105 42-43 0.06885588495065065 1105 44-45 0.045194981327234984 1105 46-47 0.20735734760837943 1105 48-49 0.06472128345686201 1105 50-51 0.11509637342117429 1105 52-53 0.12109488197361173 1105 54-55 0.09523531855452205 1105 56-57 0.06363961679271313 1105 58-59 -0.04271068191358296 1105 60-61 0.0020912938630388567 1105 62-63 0.014189054093733944 1105 64-65 0.014731442593259203 1105 66-67 0.11555848662704449 1105 68-69 0.11304476722515489 1105 70-71 0.11977670521740436 1105 72-73 0.02099295159721848 1105 74-75 0.19107785239987862 1105 76-77 -0.019431180713073815 1105 78-79 0.2077719249472949 1105 80-81 0.14519821891263973 1105 82-83 0.12973182746840806 1105 84-85 -0.06228098570341345 1105 86-87 -0.04604704293059925 1105 88-89 0.1162295132687845 1105 90-91 0.18260065613557686 1105 92-93 0.16715500798146365 1105 94-95 0.09482031994514273 1105 96-97 -0.0706698487244175 1105 98-99 0.20872584197480748 1105 100-101 0.31220392711266953 1106 1 -0.04037746440433665 1106 2 -0.0996094871355453 1106 3 -0.09717529141356351 1106 4 0.010475708491618718 1106 5 0.023347347958157627 1106 6 0.017727438043387167 1106 7 0.06936035877382807 1106 8 0.04935892674133413 1106 9 -0.03368039587699201 1106 10-11 0.060037858332549376 1106 12-13 0.035869041441735305 1106 14-15 0.027768193516095607 1106 16-17 0.009439420873057713 1106 18-19 -0.02125082654670507 1106 20-21 0.013898231260355942 1106 22-23 0.17895224186802494 1106 24-25 0.12037164307728432 1106 26-27 0.14366943405750732 1106 28-29 0.1939143062045261 1106 30-31 0.18756418877252656 1106 32-33 0.1868092594167905 1106 34-35 0.27590241395948567 1106 36-37 0.20953944662601742 1106 38-39 0.3181734076789198 1106 40-41 0.3760127932546524 1106 42-43 0.44752427923183546 1106 44-45 0.3238973540930985 1106 46-47 0.3597720692592077 1106 48-49 0.23424618146891873 1106 50-51 0.21342457499365963 1106 52-53 0.30689743848721207 1106 54-55 0.33379472077311334 1106 56-57 0.3581740740651469 1106 58-59 0.4943266911206976 1106 60-61 0.3592979865148962 1106 62-63 0.395934330619518 1106 64-65 0.3940177024797791 1106 66-67 0.41499238347940093 1106 68-69 0.4139336959672093 1106 70-71 0.51221478295286 1106 72-73 0.4031641587751089 1106 74-75 0.5547810183969162 1106 76-77 0.3341596373586775 1106 78-79 0.48140226662450303 1106 80-81 0.5655734787370861 1106 82-83 0.6467958194815147 1106 84-85 0.523164706751416 1106 86-87 0.605300715314133 1106 88-89 0.6615305857353242 1106 90-91 0.921243055226526 1106 92-93 0.8624354024557768 1106 94-95 0.855141846979631 1106 96-97 0.7819767393468844 1106 98-99 0.7510684170761088 1106 100-101 1.1510361468733912 1107 1 -0.11310523718812249 1107 2 -0.059029518952300464 1107 3 -0.14012313249191521 1107 4 -0.06759707631392331 1107 5 -0.09720442230113946 1107 6 0.009964323765075278 1107 7 -0.11837904492982432 1107 8 -0.11233952049105511 1107 9 -0.06575886369328998 1107 10-11 -0.04932593686483244 1107 12-13 -0.1207753477069815 1107 14-15 -0.103571815834961 1107 16-17 -0.08820595114990937 1107 18-19 -0.12403522678351919 1107 20-21 -0.0710326707981821 1107 22-23 -0.09504805632357005 1107 24-25 -0.15959907334973877 1107 26-27 -0.19455706417432594 1107 28-29 -0.09958019534505524 1107 30-31 -0.12096821640326993 1107 32-33 -0.1131180744752811 1107 34-35 -0.2427108214048772 1107 36-37 -0.09753189209502722 1107 38-39 -0.020414684054081533 1107 40-41 -0.12800367386972056 1107 42-43 -0.22690774538779834 1107 44-45 -0.1370456985492936 1107 46-47 -0.34369151300621326 1107 48-49 -0.24890034785590842 1107 50-51 -0.2269655493638183 1107 52-53 -0.15262515097388274 1107 54-55 -0.23124781495559432 1107 56-57 -0.15794195141721445 1107 58-59 -0.1668436240504505 1107 60-61 -0.18577323929864775 1107 62-63 -0.29812239237062244 1107 64-65 -0.34253291554402665 1107 66-67 -0.16565563318458487 1107 68-69 -0.21119111748210173 1107 70-71 -0.3284448718686477 1107 72-73 -0.1546150231087431 1107 74-75 -0.17336544578896707 1107 76-77 -0.4668213550982294 1107 78-79 -0.19178647273432858 1107 80-81 -0.051280498574293176 1107 82-83 0.004132780330120767 1107 84-85 -0.09111548623355503 1107 86-87 -0.13008073108882456 1107 88-89 0.12399282324348704 1107 90-91 0.29309051140610975 1107 92-93 0.22538574776454467 1107 94-95 0.2251869086517324 1107 96-97 0.24745223190584653 1107 98-99 0.12281280897513724 1107 100-101 0.7588842312818791 1108 1 0.00595865266382134 1108 2 -0.03254962076786683 1108 3 -0.03783860565302888 1108 4 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-1.3630689577112989 2316 60-61 -1.1664837819288678 2316 62-63 -1.2414137914652628 2316 64-65 -1.2603587685577935 2316 66-67 -1.234386259800253 2316 68-69 -1.4571787068189934 2316 70-71 -1.4075493974912305 2316 72-73 -1.4418552004373488 2316 74-75 -1.64941701846913 2316 76-77 -1.6000932784585835 2316 78-79 -1.6749809025573512 2316 80-81 -1.7263188067002986 2316 82-83 -1.8552965748066796 2316 84-85 -1.968164824760418 2316 86-87 -1.8843484317824668 2316 88-89 -2.1900109900234064 2316 90-91 -2.5216633808164595 2316 92-93 -2.7113880061978612 2316 94-95 -2.804305377085946 2316 96-97 -2.897069121219044 2316 98-99 -3.176047012526542 2316 100-101 -3.839576425395432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 19.0 5 70.0 6 215.0 7 460.0 8 952.0 9 1858.0 10 3021.0 11 4671.0 12 6936.0 13 9649.0 14 11258.0 15 15067.0 16 19694.0 17 27807.0 18 26678.0 19 17115.0 20 15299.0 21 16089.0 22 17664.0 23 19066.0 24 21802.0 25 26145.0 26 30872.0 27 37361.0 28 45741.0 29 55476.0 30 66973.0 31 81785.0 32 101218.0 33 128444.0 34 173732.0 35 250219.0 36 389965.0 37 597572.0 38 426023.0 39 29005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.297090190183916 19.44418283800917 14.325497522284802 21.93322944952211 2 15.707922105585723 22.466021158998927 37.77613764186656 24.049919093548784 3 20.988366661310273 24.901660357333714 30.837577963549123 23.272395017806893 4 12.786619978848476 16.80156057893891 37.818814393500574 32.59300504871204 5 12.721839757421566 38.68881632396807 34.256841951061276 14.33250196754908 6 30.526882242809044 36.68272338962529 16.912251067853045 15.878143299712624 7 26.90574865560758 34.30829655484261 21.037919527042934 17.748035262506868 8 25.747086059799773 33.14458898401677 19.987294136991625 21.121030819191834 9 25.503694042818758 17.575123415037012 21.813164021480382 35.10801852066384 10-11 25.182273078892198 26.020299484665145 27.074811373989604 21.722616062453053 12-13 27.166031996352668 24.023703908547684 27.379677345819957 21.43058674927969 14-15 23.00572033763707 25.501038719439617 27.686721918045244 23.80651902487807 16-17 21.12463988226593 28.696726025753954 28.035406173938032 22.14322791804208 18-19 20.751458371482066 28.22885875191055 29.936003557641644 21.083679318965743 20-21 22.548553213275387 26.03593890722104 29.192056593408644 22.223451286094928 22-23 21.73014615479478 26.212494347759467 29.724114606884335 22.333244890561414 24-25 22.738020052841442 26.281068637819377 29.554528705907853 21.42638260343133 26-27 22.16571434977746 25.454178547271418 31.117779613069104 21.262327489882022 28-29 21.034967282402754 27.763357038487552 30.071601275070726 21.13007440403897 30-31 21.621641821721795 26.33376059911881 29.65731540062707 22.387282178532324 32-33 21.69346731790443 26.637505465389605 30.011584757448812 21.657442459257155 34-35 22.719283821710324 26.199647217540463 29.60975308964935 21.471315871099865 36-37 22.294850499425294 25.674102220126837 30.613061765864995 21.417985514582867 38-39 21.688218347844806 26.686921434722287 30.327304244606033 21.297555972826867 40-41 21.76258485338669 27.01533391719427 29.666606935214045 21.555474294204995 42-43 21.31241487723169 27.30715050873262 28.615768553143234 22.764666060892456 44-45 22.01914968636003 26.235701872086164 28.90925115992882 22.83589728162498 46-47 22.72125950000729 27.246340416133673 28.585244919595848 21.447155164263194 48-49 22.451491027860484 26.91255071849054 28.64025396849124 21.995704285157736 50-51 20.894106216103854 27.544622628729453 29.63983314103133 21.921438014135365 52-53 22.005377569667367 28.237173618143974 27.65808522644464 22.099363585744022 54-55 21.169742855754823 26.980021898928598 28.47494142223451 23.375293823082067 56-57 22.21197863920207 27.059041155785092 28.061459006775213 22.667521198237623 58-59 23.21171183796257 26.715565645524446 27.819053224585893 22.253669291927093 60-61 21.972365182304703 27.14389205577938 29.3645023372829 21.519240424633022 62-63 21.39530929433879 27.201365506571545 29.011838874708978 22.391486324380683 64-65 21.034308342521747 28.288738448614097 28.40189564462512 22.275057564239038 66-67 21.644246080461485 27.205668845008148 27.59895870120173 23.55112637332864 68-69 21.04738097765075 27.311254897446975 28.30077835960004 23.340585765302233 70-71 22.168722649695617 27.224927408415017 27.754799266049563 22.851550675839803 72-73 21.837678863049483 26.32467964408636 28.929418930988476 22.908222561875686 74-75 20.91497161734425 28.520701214157324 27.648798735392926 22.9155284331055 76-77 21.40828881037166 26.910440093527217 27.72702594598276 23.954245150118368 78-79 21.449283865394758 27.195438891584732 28.12298609622791 23.2322911467926 80-81 21.912251067853045 27.012571330341228 27.8295209515944 23.245656650211327 82-83 21.407379864196745 26.764171708527503 28.546841658787788 23.281606768487965 84-85 21.059650289805788 27.021222528242518 28.169888599477567 23.74923858247413 86-87 20.948022819195337 27.137783822443517 28.01979519910657 23.89439815925457 88-89 20.96158379213479 27.065657528672904 27.33221154062701 24.640547138565296 90-91 21.14171670906913 26.17031727789329 27.804065011689584 24.883901001348004 92-93 21.5605602538183 26.877235204209377 27.769298897953238 23.79290564401909 94-95 21.105802468674444 26.457754874006422 27.946844648402614 24.489598008916527 96-97 19.920296466649837 26.432476499173273 28.795255756298822 24.851971277878064 98-99 20.67541004435841 26.907280833205654 27.285130776963523 25.13217834547242 100-101 20.44802094528843 25.681222635620443 28.260854942068608 25.609901477022518 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3033.0 1 2060.5 2 1281.5 3 1450.5 4 1259.5 5 1257.5 6 2052.0 7 3654.0 8 5347.0 9 6125.0 10 5420.5 11 4619.0 12 4747.0 13 5010.0 14 5331.0 15 5564.5 16 5697.0 17 6025.0 18 6307.5 19 6601.0 20 7165.5 21 8032.5 22 9359.0 23 11009.5 24 12416.0 25 14516.5 26 17831.5 27 21102.5 28 23148.5 29 25781.0 30 29123.5 31 32933.5 32 37124.0 33 40629.0 34 45728.5 35 50034.0 36 54355.0 37 59251.5 38 61831.5 39 62874.0 40 64305.5 41 67535.0 42 70962.0 43 75419.0 44 79806.5 45 82170.5 46 87378.5 47 115734.5 48 169244.0 49 167065.5 50 121321.0 51 100523.0 52 89820.5 53 85317.5 54 83167.5 55 78456.0 56 70828.5 57 63100.0 58 57472.0 59 51721.0 60 44288.5 61 35486.5 62 28214.5 63 22479.0 64 17355.5 65 12904.0 66 8847.0 67 6807.0 68 4892.0 69 2716.0 70 1733.0 71 1174.0 72 749.5 73 501.5 74 347.5 75 231.5 76 139.0 77 63.0 78 30.5 79 16.0 80 9.5 81 6.0 82 5.0 83 4.0 84 2.0 85 1.5 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4025890064388829 2 0.0 3 0.0 4 0.0 5 4.484422238249879E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014574372274312108 16-17 0.0025785427869936804 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022422111191249395 36-37 0.00764220289768417 38-39 0.012930084120287151 40-41 0.0024103769530593104 42-43 5.792378724406094E-4 44-45 4.2975713116561343E-4 46-47 4.11072038506239E-4 48-49 2.8027638989061744E-4 50-51 6.539782430781074E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.8685092659374497E-4 60-61 0.0024851173236968083 62-63 0.0 64-65 2.4290620457186846E-4 66-67 0.0015321775980687087 68-69 0.0017190285246624537 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1247790487793029 78-79 5.605527797812349E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14832226553011477 88-89 0.43880071601275067 90-91 0.3372659225017097 92-93 0.0 94-95 0.0 96-97 5.044975018031115E-4 98-99 0.0 100-101 2.2422111191249395E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2675930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.954179612661555 #Duplication Level Percentage of deduplicated Percentage of total 1 64.75026354449152 23.280426054458196 2 15.664053820774814 11.263764090690703 3 6.736547492568579 7.266211155511064 4 3.6657700863522096 5.271990244137168 5 2.2750828064112985 4.089936792769498 6 1.4355500204752325 3.096841396747589 7 1.0232472589879433 2.575301101647129 8 0.7298808231646945 2.099381296952055 9 0.5565342972283538 1.8008760674839146 >10 2.8806263915563326 18.331983854171487 >50 0.17880408095679684 4.425142025460612 >100 0.09415999525690937 5.975654311293041 >500 0.004479268311398352 1.0992490815647917 >1k 0.003437578006421598 2.9552759124342014 >5k 9.375212744786174E-4 2.591943192152801 >10k+ 6.25014182985745E-4 3.876023422525733 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34962 1.3065364191141025 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16842 0.6293886611383706 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14339 0.5358510872855419 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12928 0.48312175580078703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12607 0.47112592631346856 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11812 0.4414166289850631 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9027 0.3373406628723472 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8440 0.31540436409024153 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8264 0.30882721147414166 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8260 0.3086777307328667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7922 0.2960466080951295 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7542 0.28184593767400495 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7511 0.28068746192912375 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6619 0.2473532566247996 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5620 0.21002044149136936 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4517 0.16880112708478923 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4438 0.16584888244460805 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4404 0.1645782961437706 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4305 0.16087864779721445 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4273 0.15968280186701447 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3790 0.14163300235805867 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3467 0.12956243250010277 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3182 0.1189119296842593 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3072 0.1148012092991969 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3021 0.11289532984794072 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3008 0.11240951743879699 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2910 0.10874723927755958 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2754 0.10291749036783474 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2750 0.10276800962655973 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.7370185318749E-5 0.0 0.0 0.0 0.0 3 3.7370185318749E-5 0.0 0.0 0.0 0.0 4 3.7370185318749E-5 0.0 0.0 0.0 0.0 5 7.4740370637498E-5 0.0 0.0 0.0 0.0 6 7.4740370637498E-5 0.0 0.0 0.0 0.0 7 1.49480741274996E-4 0.0 0.0 0.0 0.0 8 1.49480741274996E-4 0.0 0.0 0.0 0.0 9 1.49480741274996E-4 0.0 0.0 0.0 0.0 10-11 1.49480741274996E-4 0.0 0.0 5.6055277978123495E-5 0.0 12-13 1.8685092659374497E-4 0.0 0.0 7.4740370637498E-5 0.0 14-15 1.8685092659374497E-4 0.0 0.0 7.4740370637498E-5 0.0 16-17 1.8685092659374497E-4 0.0 0.0 1.3079564861562148E-4 0.0 18-19 2.429062045718685E-4 0.0 0.0 2.429062045718685E-4 0.0 20-21 2.98961482549992E-4 0.0 0.0 3.923869458468645E-4 0.0 22-23 2.98961482549992E-4 0.0 0.0 7.660887990343544E-4 0.0 24-25 4.1107203850623895E-4 0.0 0.0 0.0018124539879593262 0.0 26-27 4.1107203850623895E-4 0.0 0.0 0.0032698912153905375 0.0 28-29 5.97922965099984E-4 0.0 0.0 0.010706558093821587 0.0 30-31 6.72663335737482E-4 0.0 0.0 0.030232479922867938 0.0 32-33 6.72663335737482E-4 0.0 0.0 0.06668709570130758 0.0 34-35 7.100335210562309E-4 0.0 0.0 0.12158389793454985 0.0 36-37 8.221440770124779E-4 0.0 0.0 0.2032564379486758 0.0 38-39 8.595142623312269E-4 0.0 0.0 0.330595344422313 0.0 40-41 8.781993549906014E-4 0.0 0.0 0.4665854487972406 0.0 42-43 9.903099109468483E-4 0.0 0.0 0.6051167257738431 0.0 44-45 0.0012705863008374658 0.0 0.0 0.7736936317467198 0.0 46-47 0.0015134925054093341 0.0 0.0 0.9804254969300392 0.0 48-49 0.0015321775980687087 0.0 0.0 1.1850272615501902 0.0 50-51 0.0015321775980687087 0.0 0.0 1.4093231138333215 0.0 52-53 0.0015321775980687087 0.0 0.0 1.623697181914325 0.0 54-55 0.0016069179687062069 0.0 0.0 1.870415145388706 0.0 56-57 0.0018685092659374498 0.0 0.0 2.1875572230962694 0.0 58-59 0.0018685092659374498 0.0 0.0 2.5113698788832295 0.0 60-61 0.0018871943585968241 0.0 0.0 2.8731693280466977 0.0 62-63 0.0019058794512561987 0.0 0.0 3.2345577051716603 0.0 64-65 0.0019058794512561987 0.0 0.0 3.5991038629560563 0.0 66-67 0.0020927303778499437 0.0 0.0 4.01959318816262 0.0 68-69 0.002130100563168693 0.0 0.0 4.51338413187191 1.86850926593745E-5 70-71 0.0021861558411468162 0.0 0.0 5.035894062998658 3.7370185318749E-5 72-73 0.002223526026465565 0.0 0.0 5.552368709196429 3.7370185318749E-5 74-75 0.0022422111191249395 0.0 0.0 6.107745718311017 3.7370185318749E-5 76-77 0.0022422111191249395 0.0 0.0 6.661086052325734 3.7370185318749E-5 78-79 0.0022422111191249395 0.0 0.0 7.357647621574555 3.7370185318749E-5 80-81 0.0026159129723124295 0.0 0.0 8.103986277667952 3.7370185318749E-5 82-83 0.0026159129723124295 0.0 0.0 8.813066858998553 3.7370185318749E-5 84-85 0.002634598064971804 0.0 0.0 9.549110029036633 3.7370185318749E-5 86-87 0.0026719682502905533 0.0 0.0 10.320337228552317 3.7370185318749E-5 88-89 0.002690653342949928 0.0 0.0 11.150852227076193 3.7370185318749E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 895 0.0 27.570517 3 CGTACCG 70 0.00843407 27.116056 5 GTACATG 11945 0.0 25.603334 1 TACATGG 12055 0.0 24.799215 2 CTTTGCG 1000 0.0 24.20108 2 ACATGGG 12085 0.0 23.677467 3 GAGTACT 12485 0.0 22.197968 12-13 AGTACTT 12470 0.0 21.444523 12-13 CATGGGA 7290 0.0 21.155361 4 CCGTCCG 135 3.8226985E-4 21.09066 9 GTACTTT 13120 0.0 20.834236 14-15 CATGGGG 4655 0.0 20.286074 4 AATACCG 165 6.374995E-5 20.131618 5 ATACCGT 155 9.693166E-4 18.368942 6 ATGGGAG 3215 0.0 18.007088 5 AGTACAT 8990 0.0 17.682747 2 ACTTTTT 15665 0.0 17.56994 16-17 TACTTTT 15250 0.0 17.473055 14-15 TTGCGTT 1440 0.0 17.465378 4 AGAGTAC 22795 0.0 16.987892 10-11 >>END_MODULE