FastQCFastQC Report
Fri 27 May 2016
SRR939041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3473404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT238640.687049361375757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141840.40836021378451803No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT125040.3599926757728154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113300.3261929795670184No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT102870.29616479971808635No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC87730.2525764351051591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72570.20893049008983694No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT64510.18572558792469865No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT57640.16594671970205596No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC52650.15158040930453237No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC51690.14881654998957797No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49500.14251149592733814No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG47300.1361776516639009No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC46050.13257887651422065No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA46050.13257887651422065No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA45100.12984380740046364No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35710.10280980847606556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG143550.024.3039471
TACATGG143500.023.7977662
ACATGGG149500.021.8922143
GAGTACT118150.021.31737112-13
AGTACTT123850.019.93489812-13
GTACTTT127650.019.7313114-15
CATGGGG61700.017.5793634
CATGGGA97850.017.4742774
TATCACG2453.8701E-617.3991992
GTATAGG8850.017.1632441
TTTGCGT10600.016.5328873
TACTTTT149500.016.4516514-15
AGTACAT110250.016.2822132
ACTTTTT159300.016.17072916-17
GTAAGCG4400.015.67460194-95
AGAGTAC254500.015.18849610-11
CTTTGCG11550.015.17303852
GCTTATA17300.014.816321
ATGGGAG40650.014.7977615
GGACCGT4502.401066E-914.7288266
Produced by FastQC (version 0.11.5)