##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939041_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3473404 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685768197422473 33.0 31.0 34.0 30.0 34.0 2 31.747684692019703 33.0 31.0 34.0 30.0 34.0 3 31.799854839805562 34.0 31.0 34.0 30.0 34.0 4 35.333569317015815 37.0 35.0 37.0 33.0 37.0 5 35.3787097613753 37.0 35.0 37.0 33.0 37.0 6 35.46917865010808 37.0 35.0 37.0 33.0 37.0 7 35.387044236719944 37.0 35.0 37.0 33.0 37.0 8 35.37505686064736 37.0 35.0 37.0 33.0 37.0 9 36.9518682537361 39.0 37.0 39.0 33.0 39.0 10-11 36.83000134738141 39.0 37.0 39.0 33.0 39.0 12-13 36.717410355950534 39.0 37.0 39.0 32.0 39.0 14-15 37.873816146926764 40.0 38.0 41.0 32.0 41.0 16-17 37.79298780101595 40.0 38.0 41.0 32.0 41.0 18-19 37.713241102964126 40.0 37.5 41.0 32.0 41.0 20-21 37.83580789910992 40.0 38.0 41.0 32.0 41.0 22-23 37.771358730513356 40.0 38.0 41.0 32.0 41.0 24-25 37.67893729033536 40.0 37.5 41.0 32.0 41.0 26-27 37.54566169095216 40.0 37.0 41.0 32.0 41.0 28-29 37.41832335081091 40.0 37.0 41.0 31.5 41.0 30-31 37.227241345953416 40.0 37.0 41.0 31.0 41.0 32-33 37.012491636446555 40.0 36.0 41.0 30.5 41.0 34-35 36.67313448133301 39.5 36.0 41.0 30.0 41.0 36-37 36.39770236920324 39.0 35.5 41.0 30.0 41.0 38-39 36.15189249508551 39.0 35.0 41.0 29.5 41.0 40-41 36.009099862843485 39.0 35.0 41.0 29.0 41.0 42-43 35.79393845345949 39.0 35.0 40.5 27.5 41.0 44-45 35.71595702659408 39.0 35.0 41.0 27.0 41.0 46-47 35.48983533156523 39.0 35.0 41.0 26.0 41.0 48-49 35.23318162816649 39.0 35.0 41.0 25.0 41.0 50-51 34.11671547565443 38.0 34.0 40.0 23.5 40.5 52-53 34.22968289896597 38.0 33.5 39.5 23.5 40.5 54-55 34.72736125714141 38.5 34.0 40.0 24.0 41.0 56-57 34.61737549101689 38.0 34.0 40.0 23.0 41.0 58-59 34.4011445256584 38.0 34.0 40.0 23.0 41.0 60-61 34.06215962784634 38.0 34.0 40.0 22.0 41.0 62-63 33.68474067514174 37.5 33.0 40.0 21.5 41.0 64-65 33.29147948237521 37.0 33.0 40.0 20.0 41.0 66-67 32.85067055257609 36.0 32.0 39.0 18.5 41.0 68-69 32.39923184864185 36.0 32.0 39.0 16.5 40.5 70-71 31.926194735769293 35.0 31.0 38.5 13.0 40.0 72-73 31.473810273725718 35.0 31.0 37.5 9.5 40.0 74-75 31.00580180134531 35.0 31.0 37.0 7.5 39.0 76-77 30.562773722837886 35.0 30.0 36.5 4.5 39.0 78-79 30.146442509998835 34.5 30.0 36.0 2.0 38.5 80-81 29.672209596119544 34.0 29.5 35.5 2.0 37.5 82-83 29.190580623503628 34.0 29.0 35.0 2.0 37.0 84-85 28.753063709260424 34.0 29.0 35.0 2.0 36.5 86-87 28.30665249421029 34.0 28.5 35.0 2.0 36.0 88-89 27.802306037535512 34.0 27.0 35.0 2.0 36.0 90-91 27.311393232690467 33.5 26.0 35.0 2.0 35.0 92-93 26.777023922353976 33.0 25.0 35.0 2.0 35.0 94-95 26.207943705943794 33.0 24.5 35.0 2.0 35.0 96-97 25.515020999572755 32.5 21.5 35.0 2.0 35.0 98-99 24.622075491362363 32.0 10.0 34.5 2.0 35.0 100-101 21.377652585187327 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18916963065052173 1101 2 0.3351347776845728 1101 3 0.34591219285658426 1101 4 0.32470714999572436 1101 5 0.17022015101969856 1101 6 0.27587858219377637 1101 7 0.3343177033949516 1101 8 0.3377950959146787 1101 9 0.4191428044501393 1101 10-11 0.3550437494730474 1101 12-13 0.4606358248029423 1101 14-15 0.5731502872284722 1101 16-17 0.5718656616574762 1101 18-19 0.5821541354386213 1101 20-21 0.5603193359343166 1101 22-23 0.6648567226904163 1101 24-25 0.5863325428962156 1101 26-27 0.6092769475336652 1101 28-29 0.5742006992052353 1101 30-31 0.5246565044971234 1101 32-33 0.5443702365172953 1101 34-35 0.45509141200125924 1101 36-37 0.5219798333692225 1101 38-39 0.6577744327813804 1101 40-41 0.5161245950781534 1101 42-43 0.4721473726243417 1101 44-45 0.5512309815406198 1101 46-47 0.4534468406951433 1101 48-49 0.3964807512223345 1101 50-51 0.4584010131842646 1101 52-53 0.4240953763490367 1101 54-55 0.6423180861494657 1101 56-57 0.6931962811893442 1101 58-59 0.589710267560811 1101 60-61 0.6847186608706295 1101 62-63 0.6227322125088577 1101 64-65 0.5447223647237394 1101 66-67 0.46640361342883097 1101 68-69 0.4358505818631002 1101 70-71 0.5338598102033103 1101 72-73 0.44967714658922375 1101 74-75 0.27265832271586987 1101 76-77 0.254770397954708 1101 78-79 0.26967968954972577 1101 80-81 0.21998261760695925 1101 82-83 0.2509750858296016 1101 84-85 0.13940053614834724 1101 86-87 0.08003255207403015 1101 88-89 0.03858153604839387 1101 90-91 0.004829866190693366 1101 92-93 -0.07807727671011122 1101 94-95 -0.1592768351063505 1101 96-97 -0.23521272102190238 1101 98-99 -0.342749376000544 1101 100-101 0.0719696987626115 1102 1 0.28969459664163466 1102 2 0.29732288257191897 1102 3 0.38511809742000835 1102 4 0.20767737673822495 1102 5 0.3005289213804403 1102 6 0.3055812714939137 1102 7 0.33505943646539293 1102 8 0.3997583797441706 1102 9 0.489578490171624 1102 10-11 0.4462920183470658 1102 12-13 0.47756394534568614 1102 14-15 0.49425586261428833 1102 16-17 0.5186115571007335 1102 18-19 0.47375563838772194 1102 20-21 0.3918467883036598 1102 22-23 0.46546744437674903 1102 24-25 0.42690159291410623 1102 26-27 0.4634307102565529 1102 28-29 0.39901237992864935 1102 30-31 0.4808420942420284 1102 32-33 0.42979903608713954 1102 34-35 0.45454354969974275 1102 36-37 0.45061851540084064 1102 38-39 0.4490062005874762 1102 40-41 0.5235859350223961 1102 42-43 0.3670883523026234 1102 44-45 0.48280557844858407 1102 46-47 0.566430448547699 1102 48-49 0.6114362606613994 1102 50-51 0.5837821389540423 1102 52-53 0.56817232712811 1102 54-55 0.6873994639320671 1102 56-57 0.6478347879510409 1102 58-59 0.5005762639319187 1102 60-61 0.649573112055279 1102 62-63 0.673582348295092 1102 64-65 0.6133338243079152 1102 66-67 0.5525870803751118 1102 68-69 0.5312094000299652 1102 70-71 0.4813650807652152 1102 72-73 0.4022838455050426 1102 74-75 0.430768324338171 1102 76-77 0.36417487527642933 1102 78-79 0.44975073331082527 1102 80-81 0.3848947314426603 1102 82-83 0.27411761785471356 1102 84-85 0.23957534583475137 1102 86-87 0.26748468363615174 1102 88-89 0.010575839200914317 1102 90-91 0.12105404983367762 1102 92-93 0.06954222611395977 1102 94-95 0.04845415970957134 1102 96-97 0.056084533300115424 1102 98-99 -0.21460031833520787 1102 100-101 0.132049262482429 1103 1 0.32568067147873947 1103 2 0.30655043222004963 1103 3 0.4174018788865048 1103 4 0.3921459571008725 1103 5 0.3127092564984011 1103 6 0.4231303454880688 1103 7 0.30211949101169466 1103 8 0.3567329190986115 1103 9 0.4797640715597211 1103 10-11 0.48612349170944924 1103 12-13 0.42576662825128153 1103 14-15 0.4454743999170603 1103 16-17 0.528346557342168 1103 18-19 0.576090414907938 1103 20-21 0.661910311593445 1103 22-23 0.5586109822213388 1103 24-25 0.6607896444749315 1103 26-27 0.7299182681402456 1103 28-29 0.6265286637838798 1103 30-31 0.6424205987546685 1103 32-33 0.5890649498936327 1103 34-35 0.5323305879372882 1103 36-37 0.5521976054516671 1103 38-39 0.7597886773303912 1103 40-41 0.6735094728593793 1103 42-43 0.689756446440434 1103 44-45 0.8117806603764066 1103 46-47 0.8346365040012031 1103 48-49 0.8136822939252966 1103 50-51 0.811429265881614 1103 52-53 0.667263735343667 1103 54-55 0.711343793698056 1103 56-57 0.6132773407606891 1103 58-59 0.5445110672956446 1103 60-61 0.8229727392119202 1103 62-63 0.7332882332201152 1103 64-65 0.7090681785562367 1103 66-67 0.769562678641158 1103 68-69 0.8049387156080527 1103 70-71 0.7206557677802223 1103 72-73 0.6703379556980025 1103 74-75 0.5873906187429725 1103 76-77 0.6433826801203502 1103 78-79 0.6412665890387785 1103 80-81 0.5818678791933358 1103 82-83 0.818750646999689 1103 84-85 0.6378658404586375 1103 86-87 0.6914932324937979 1103 88-89 0.763753904788036 1103 90-91 0.8736475277746649 1103 92-93 0.6020095110684025 1103 94-95 0.5753631568081126 1103 96-97 0.5875131058150274 1103 98-99 0.3728134050831642 1103 100-101 0.41952403966423546 1104 1 0.30984625034203006 1104 2 0.3319788188054815 1104 3 0.2590597344554091 1104 4 0.17529862456638057 1104 5 0.2442213310689425 1104 6 0.2429622021208786 1104 7 0.2409577263969993 1104 8 0.28885877971909935 1104 9 0.3681683161443985 1104 10-11 0.38110571324101983 1104 12-13 0.3874308368299282 1104 14-15 0.4385367761005483 1104 16-17 0.42787937342789206 1104 18-19 0.47027841626911027 1104 20-21 0.46799436598993793 1104 22-23 0.4519929255057349 1104 24-25 0.46740377401206246 1104 26-27 0.4238326635417238 1104 28-29 0.5243820943433732 1104 30-31 0.5983148144717632 1104 32-33 0.6497724113635925 1104 34-35 0.6315065944928548 1104 36-37 0.6570607395242263 1104 38-39 0.6380908190331382 1104 40-41 0.6047456584454309 1104 42-43 0.693870494709401 1104 44-45 0.7250561113583629 1104 46-47 0.7176346773934696 1104 48-49 0.7526411162377542 1104 50-51 0.6114459704828263 1104 52-53 0.7411830049561132 1104 54-55 0.7485995170909021 1104 56-57 0.6955509779123261 1104 58-59 0.7069317462766165 1104 60-61 0.8060879387642643 1104 62-63 0.8674012367468649 1104 64-65 0.8727581911401217 1104 66-67 0.7910564645858855 1104 68-69 0.8308423095776405 1104 70-71 0.7117762575613824 1104 72-73 0.7155574896715748 1104 74-75 0.566602268670902 1104 76-77 0.681457990625816 1104 78-79 0.6373598920344996 1104 80-81 0.6486733656683406 1104 82-83 0.8003113697282984 1104 84-85 0.6919579792056645 1104 86-87 0.7398664410944349 1104 88-89 0.5991649629333899 1104 90-91 0.6959551509917681 1104 92-93 0.6371036891296846 1104 94-95 0.6849290870881077 1104 96-97 0.49305506865640325 1104 98-99 0.44666859967861683 1104 100-101 0.8227753676236595 1105 1 0.1595932669952944 1105 2 0.24030025341137673 1105 3 0.25655775236569767 1105 4 0.2530106524623221 1105 5 0.19370635909671563 1105 6 0.29504854881854214 1105 7 0.19861082077789405 1105 8 0.2399551027802289 1105 9 0.22700583677762864 1105 10-11 0.3319368911871905 1105 12-13 0.35333443764170624 1105 14-15 0.39443254139141715 1105 16-17 0.4405673470173568 1105 18-19 0.40782411573788835 1105 20-21 0.48379677346301975 1105 22-23 0.3566269305524088 1105 24-25 0.4304980063631092 1105 26-27 0.33916438273299576 1105 28-29 0.3694560211833746 1105 30-31 0.3711612995350677 1105 32-33 0.48511348861774906 1105 34-35 0.5022907778811856 1105 36-37 0.5085945148898645 1105 38-39 0.6490894208939864 1105 40-41 0.7559704691953186 1105 42-43 0.6350756795218544 1105 44-45 0.7227425902572335 1105 46-47 0.7407089867257213 1105 48-49 0.7621677727912797 1105 50-51 0.6597368242760879 1105 52-53 0.5199128150282917 1105 54-55 0.5801760164698138 1105 56-57 0.47991790681518864 1105 58-59 0.4931280464996135 1105 60-61 0.7109408556390377 1105 62-63 0.6680424682976494 1105 64-65 0.6720379545148205 1105 66-67 0.6360811873254164 1105 68-69 0.661123812735056 1105 70-71 0.5799555016007218 1105 72-73 0.5477847829075913 1105 74-75 0.39062993065915563 1105 76-77 0.4112984207996284 1105 78-79 0.4603054351642193 1105 80-81 0.5078111241523366 1105 82-83 0.5918583837821032 1105 84-85 0.5295244743697367 1105 86-87 0.5005930278338369 1105 88-89 0.6693280606007441 1105 90-91 0.7450519308154888 1105 92-93 0.8283215927379217 1105 94-95 0.7872177276252295 1105 96-97 0.8351810200130458 1105 98-99 0.7862371447040779 1105 100-101 0.955906345225582 1106 1 0.18850955474318098 1106 2 0.14134671325324177 1106 3 0.1668703297481251 1106 4 0.19586688827270393 1106 5 0.10036580567120268 1106 6 0.16030094818397345 1106 7 0.14556191916616257 1106 8 0.08282385583584073 1106 9 0.21236238325153067 1106 10-11 0.2585688270335922 1106 12-13 0.27269566810505097 1106 14-15 0.25009627739002127 1106 16-17 0.25659177922876353 1106 18-19 0.2811641974990877 1106 20-21 0.21701935638750314 1106 22-23 0.38187267219465326 1106 24-25 0.29520073539468683 1106 26-27 0.23440197909537375 1106 28-29 0.1756536902546273 1106 30-31 0.06949319519929276 1106 32-33 0.15677508035781784 1106 34-35 0.22057062720257647 1106 36-37 0.21599932713572656 1106 38-39 0.3554657781760753 1106 40-41 0.4002130806192099 1106 42-43 0.32186418070872946 1106 44-45 0.40123329166741684 1106 46-47 0.26518419214912825 1106 48-49 0.3389845194141188 1106 50-51 0.13076897092015116 1106 52-53 0.11724841053623436 1106 54-55 0.21742924306188627 1106 56-57 0.16718582756470113 1106 58-59 0.3110444875383962 1106 60-61 0.45814869361146293 1106 62-63 0.4012147101271992 1106 64-65 0.21677009907267575 1106 66-67 0.19427776068418012 1106 68-69 0.2903919439194098 1106 70-71 0.38845575421324696 1106 72-73 0.24309852584509883 1106 74-75 0.23238165563532576 1106 76-77 0.10377551106619975 1106 78-79 0.2179010446951004 1106 80-81 0.2172605104898544 1106 82-83 0.3576096588630797 1106 84-85 0.43050095555441104 1106 86-87 0.5295349396525637 1106 88-89 0.5682584710666099 1106 90-91 0.5454718481109673 1106 92-93 0.49515411290384037 1106 94-95 0.5522407348661851 1106 96-97 0.6184050995646722 1106 98-99 0.6678413748073311 1106 100-101 0.6615281756640776 1107 1 0.04854073138235648 1107 2 0.17990403678274092 1107 3 0.14365052922786603 1107 4 0.14697180970802748 1107 5 0.1996835972639417 1107 6 0.14734216902625974 1107 7 0.04025499055872217 1107 8 0.14065523537171032 1107 9 0.20502167340509203 1107 10-11 0.2367684256547804 1107 12-13 0.25674605718734966 1107 14-15 0.31243621642368424 1107 16-17 0.2425078915098524 1107 18-19 0.266945135116778 1107 20-21 0.2336901160960494 1107 22-23 0.2709966430466295 1107 24-25 0.2907294130188802 1107 26-27 0.2291365544540085 1107 28-29 0.36172402592055874 1107 30-31 0.4320799159810136 1107 32-33 0.3504775487140819 1107 34-35 0.4449310655108789 1107 36-37 0.39991490653925865 1107 38-39 0.49390153575467366 1107 40-41 0.33532116038816895 1107 42-43 0.44668079765927615 1107 44-45 0.3081618643498487 1107 46-47 0.5410606462435439 1107 48-49 0.7060269638239092 1107 50-51 0.6304526943612174 1107 52-53 0.7887108882917175 1107 54-55 0.7784093102086302 1107 56-57 0.6849720498121741 1107 58-59 0.6436988625656639 1107 60-61 0.6838284644428327 1107 62-63 0.6605277708239683 1107 64-65 0.639882307339164 1107 66-67 0.5756555846006535 1107 68-69 0.7415183299268051 1107 70-71 0.6952058233430733 1107 72-73 0.7759179868948962 1107 74-75 0.8243221520178885 1107 76-77 0.7991778953195805 1107 78-79 0.9356733366960093 1107 80-81 0.9214259149180215 1107 82-83 0.9045329079840343 1107 84-85 0.9311652275743256 1107 86-87 1.1186971643694292 1107 88-89 1.0309838077953444 1107 90-91 1.0673249984583855 1107 92-93 0.9612356136232947 1107 94-95 1.0094183921758635 1107 96-97 1.0496781902736068 1107 98-99 0.9228785971561386 1107 100-101 0.9644495560637374 1108 1 0.2620744017719403 1108 2 0.2901012720541658 1108 3 0.26739190721022155 1108 4 0.3556628356613345 1108 5 0.2628028335779504 1108 6 0.28804004023207597 1108 7 0.3715733253595488 1108 8 0.35612244297725937 1108 9 0.3755587801814926 1108 10-11 0.47178693003215955 1108 12-13 0.43254930299936234 1108 14-15 0.6064572268311323 1108 16-17 0.6802544981471286 1108 18-19 0.6561506111758248 1108 20-21 0.40775064075220513 1108 22-23 0.496481492760779 1108 24-25 0.5446475534205462 1108 26-27 0.6885012669843178 1108 28-29 0.7423094054519552 1108 30-31 0.6997171994465603 1108 32-33 0.7086942017423112 1108 34-35 0.789766423999346 1108 36-37 0.7981662969365004 1108 38-39 0.8194294006181195 1108 40-41 0.8197858469521293 1108 42-43 0.9018814668155883 1108 44-45 0.8150026491448941 1108 46-47 0.984842464184915 1108 48-49 1.0734807046675598 1108 50-51 1.0101370253559097 1108 52-53 0.9844935272201951 1108 54-55 1.1011846612235061 1108 56-57 1.1633515313536407 1108 58-59 1.173106427620496 1108 60-61 1.06847687233725 1108 62-63 1.1677398226430213 1108 64-65 1.248228881487215 1108 66-67 1.2285186833033563 1108 68-69 1.255236445416493 1108 70-71 1.234335982681415 1108 72-73 1.2190110620634727 1108 74-75 1.2169116979605512 1108 76-77 1.2499006642013448 1108 78-79 1.2193470323871516 1108 80-81 1.3541342359279085 1108 82-83 1.3587007310439745 1108 84-85 1.4498417906442356 1108 86-87 1.4482536561989612 1108 88-89 1.4758900152906307 1108 90-91 1.4320527636739477 1108 92-93 1.5820138697244488 1108 94-95 1.506060101001367 1108 96-97 1.5905942735610878 1108 98-99 1.6400521587265082 1108 100-101 1.55544808654329 1109 1 -0.014474746835006158 1109 2 -0.029009053942740337 1109 3 0.06794085102481873 1109 4 0.12101248919211827 1109 5 0.1014432406996022 1109 6 0.0873068697870707 1109 7 0.10489442419503092 1109 8 0.10381247522859383 1109 9 0.1928877240860274 1109 10-11 0.08545100762078306 1109 12-13 0.08210847190219539 1109 14-15 0.16515734718122843 1109 16-17 0.08292624264930026 1109 18-19 0.1699674354899301 1109 20-21 0.15405408697155565 1109 22-23 0.21640897309188745 1109 24-25 0.14933596635940205 1109 26-27 0.0781437469366395 1109 28-29 0.14022803024366937 1109 30-31 0.19782849163438954 1109 32-33 0.2728623123910623 1109 34-35 0.10663536346902447 1109 36-37 0.15314369367289515 1109 38-39 0.29028571111099666 1109 40-41 0.37213429110106233 1109 42-43 0.19487151466850605 1109 44-45 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2316 50-51 -2.1917112650998476 2316 52-53 -2.2967526656519937 2316 54-55 -2.143531173657358 2316 56-57 -1.969085864621519 2316 58-59 -1.7062569348465146 2316 60-61 -1.920604010225901 2316 62-63 -2.1436474606448215 2316 64-65 -2.263784086533864 2316 66-67 -2.3360371198992738 2316 68-69 -2.4788351182507 2316 70-71 -2.3067011350096607 2316 72-73 -2.29959198463569 2316 74-75 -2.2491150144974448 2316 76-77 -2.3542620594232027 2316 78-79 -2.321675534837105 2316 80-81 -2.2604035935998503 2316 82-83 -2.156494281129305 2316 84-85 -2.2104918922423735 2316 86-87 -2.2355141582270974 2316 88-89 -2.477952642045363 2316 90-91 -2.730437796848154 2316 92-93 -2.724555779658413 2316 94-95 -2.70979409496476 2316 96-97 -2.818129915120277 2316 98-99 -2.778656472317195 2316 100-101 -2.5664859248540175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10318.0 3 3167.0 4 3286.0 5 3739.0 6 4437.0 7 5479.0 8 6672.0 9 8405.0 10 10559.0 11 13210.0 12 17176.0 13 23344.0 14 21457.0 15 21815.0 16 26357.0 17 33919.0 18 30512.0 19 23608.0 20 24145.0 21 25703.0 22 28350.0 23 31166.0 24 35307.0 25 40170.0 26 46353.0 27 53917.0 28 62815.0 29 73737.0 30 87553.0 31 105026.0 32 130013.0 33 165421.0 34 218303.0 35 307795.0 36 458649.0 37 669231.0 38 562132.0 39 80145.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08072634210522 18.585790603999705 13.586231100186163 22.747251953708904 2 15.1997766188005 20.868852586562376 39.728484647297165 24.202886147339957 3 20.51228699138023 24.53176288858853 29.78054312334831 25.17540699668293 4 12.653346959690998 16.052356570192746 37.770343996361696 33.52395247375456 5 13.349586273510003 37.92350145748981 34.269472326141056 14.457439942859136 6 31.646023970397813 35.86721649840913 17.412401173601737 15.074358357591322 7 27.965973525169414 33.1910810282151 20.952600713796603 17.890344732818885 8 25.745981867123906 34.3104701918379 20.03085907611752 19.912688864920675 9 25.977579194912582 17.395930053172563 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22.08326118455001 34-35 22.70874493085162 26.48153443554816 28.93699525146442 21.8727253821358 36-37 22.42190160735362 26.218131217394802 29.512079096532368 21.847888078719212 38-39 22.160074958567687 26.76478220156368 29.422481726824156 21.652661113044473 40-41 22.183203945398063 26.935298416064917 29.06318198731365 21.818315651223365 42-43 21.741753149315674 27.14198526772818 28.48581576544902 22.630445817507134 44-45 22.19981094280629 26.610552307993668 28.530897714965754 22.658739034234284 46-47 22.657816546280205 27.161737575114657 28.167234928570284 22.013210950034857 48-49 22.541976801439134 26.98945521276006 28.261010794231016 22.207557191569784 50-51 21.760720891003317 27.398577680730952 28.811391286243598 22.029310142022137 52-53 22.385823398406796 27.74308070906157 27.707937538620957 22.16315835391068 54-55 21.7537326172387 27.044599285795712 28.268083540258914 22.933584556706673 56-57 22.490253238615036 27.18634448175289 27.840004859820922 22.483397419811148 58-59 22.99336874917299 26.914172626549444 27.789995206063356 22.30246341821421 60-61 22.3788420039828 26.93707659606947 28.80126815909927 21.88281324084846 62-63 22.12526023795499 27.023433277823617 28.569585227857928 22.28172125636346 64-65 21.838827169970095 27.664764819497663 28.227071668042903 22.269336342489336 66-67 22.075483711325887 27.11385213793822 27.777670053236825 23.03299409749907 68-69 21.704297075180108 27.23750190490511 28.209325518145047 22.848875501769736 70-71 22.4662849574499 27.0861589598337 27.756205090266644 22.691350992449753 72-73 22.252069341591742 26.634545802618607 28.39329261719347 22.720092238596173 74-75 21.75043931950046 27.874489486430292 27.773761454720574 22.601309739348675 76-77 22.15453855651848 26.88015786157744 27.796339468938726 23.16896411296535 78-79 22.12808641305684 27.022912246140674 28.038870254578907 22.81013108622358 80-81 22.385630912752603 27.089362437061908 27.858917996378306 22.666088653807183 82-83 22.16813231164931 26.885720196100838 28.220655395738607 22.72549209651124 84-85 22.081130778690618 27.039441068411573 27.952423807076247 22.92700434582156 86-87 22.01704387229696 27.078040184699574 28.00111853214789 22.903797410855578 88-89 22.130045426926305 26.922065333757704 27.549246260235527 23.398642979080467 90-91 22.192928602940338 26.461027099146015 27.877254060082613 23.468790237831037 92-93 22.481286335027235 26.914324254047145 27.840099183039328 22.76429022788629 94-95 22.285796232522372 26.68970535161031 27.86768244875916 23.156815967108155 96-97 21.69469016432677 26.519703556048295 28.390160246309282 23.39544603331565 98-99 22.136572705427884 26.80162796304062 27.496663879693035 23.56513545183846 100-101 22.38513063858106 25.747663551401867 28.21692080668962 23.65028500332745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3801.0 1 2350.0 2 1034.0 3 1217.5 4 1099.0 5 1116.5 6 1870.0 7 3363.5 8 5167.5 9 5983.0 10 5344.5 11 4760.5 12 4852.5 13 5179.0 14 5570.5 15 5928.0 16 6352.5 17 6665.5 18 6885.5 19 7205.0 20 7684.0 21 8587.0 22 9998.0 23 11862.0 24 14045.5 25 16857.5 26 20862.0 27 25478.5 28 29427.0 29 33530.5 30 38842.0 31 44409.5 32 49239.5 33 54390.0 34 61119.0 35 67536.0 36 74806.0 37 82171.5 38 86875.0 39 89444.5 40 92119.5 41 96638.0 42 100979.5 43 106207.0 44 112614.5 45 117234.0 46 122417.0 47 142050.0 48 185891.0 49 185986.5 50 145475.0 51 127764.5 52 119967.5 53 116215.0 54 112389.5 55 105255.5 56 96341.5 57 86621.0 58 77570.5 59 68131.5 60 57488.0 61 46447.0 62 36469.5 63 28465.5 64 21419.5 65 15509.0 66 11205.0 67 8600.5 68 6259.5 69 4040.5 70 2739.5 71 1896.0 72 1287.0 73 881.0 74 594.5 75 376.0 76 241.5 77 140.5 78 90.0 79 67.0 80 51.5 81 46.0 82 38.0 83 29.0 84 22.0 85 20.5 86 22.0 87 18.0 88 13.0 89 8.5 90 5.0 91 5.5 92 8.5 93 6.0 94 3.5 95 3.0 96 1.5 97 3.0 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3281795034496419 2 0.08933599431566268 3 0.10001715895991367 4 0.10531455598024303 5 0.11755039148915589 6 0.058703220241584336 7 0.060660953923010394 8 0.06161103056252598 9 0.06486432329783694 10-11 0.062143649284678665 12-13 0.06048821271582574 14-15 0.05830015742482015 16-17 0.0604594225146283 18-19 0.06893813676727499 20-21 0.0704496223301407 22-23 0.08724870472884813 24-25 0.09574181408209353 26-27 0.09099143088451558 28-29 0.08868821478872023 30-31 0.08295896475042926 32-33 0.07390444647383373 34-35 0.3248686303119361 36-37 0.5031087659252997 38-39 0.4767945220308377 40-41 0.45798012554830936 42-43 0.4888144310307698 44-45 0.4982720121241295 46-47 0.4924419963816475 48-49 0.5100328093132847 50-51 0.5058294399384581 52-53 0.5049945241037322 54-55 0.49663097065587536 56-57 0.47464965204162834 58-59 0.45809528635309915 60-61 0.41837920380122784 62-63 0.4880514906990376 64-65 0.5104070819288513 66-67 0.5207859494605293 68-69 0.5328346486616586 70-71 0.5302579256544876 72-73 0.5187274500749122 74-75 0.5199510336258034 76-77 0.4955225479097738 78-79 0.4755853335805452 80-81 0.4817608317373965 82-83 0.48998043417926623 84-85 0.4921828845708705 86-87 0.4919813531624884 88-89 0.4981856415205372 90-91 0.5118465919887235 92-93 0.517489471423422 94-95 0.5240104519946427 96-97 0.5156612936473846 98-99 0.5194616002054468 100-101 0.4981856415205372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3473404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.21985929467887 #Duplication Level Percentage of deduplicated Percentage of total 1 70.88526007010393 34.1807726664713 2 14.589731022356553 14.070295540904883 3 5.724965636450913 8.281711123696041 4 2.9108566496458943 5.614443922916214 5 1.6297300944596478 3.929267792157396 6 1.0334573388672974 2.9899900480340604 7 0.6545510140209737 2.2093650458096508 8 0.4841122164875902 1.8675058369493371 9 0.34805555967648677 1.5104871102897806 >10 1.615675006854787 13.414885565756393 >50 0.08194365587265284 2.7188600019355893 >100 0.036999694177654 3.115355521959991 >500 0.0021517112458819187 0.705881302198623 >1k 0.0018528624564655906 2.196440222173715 >5k 3.586185399610821E-4 1.1147633034176239 >10k+ 2.988487833009018E-4 2.0799749953294167 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23864 0.687049361375757 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14184 0.40836021378451803 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12504 0.3599926757728154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11330 0.3261929795670184 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10287 0.29616479971808635 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8773 0.2525764351051591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7257 0.20893049008983694 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6451 0.18572558792469865 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5764 0.16594671970205596 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5265 0.15158040930453237 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5169 0.14881654998957797 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4950 0.14251149592733814 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4730 0.1361776516639009 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4605 0.13257887651422065 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4605 0.13257887651422065 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4510 0.12984380740046364 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3571 0.10280980847606556 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.879020119744205E-5 0.0 12-13 0.0 0.0 0.0 4.318530179616307E-5 0.0 14-15 2.879020119744205E-5 0.0 0.0 8.637060359232615E-5 0.0 16-17 2.879020119744205E-5 0.0 0.0 1.4395100598721023E-4 0.0 18-19 4.318530179616307E-5 0.0 0.0 1.871363077833733E-4 0.0 20-21 5.75804023948841E-5 0.0 0.0 3.3108731377058355E-4 0.0 22-23 5.75804023948841E-5 0.0 0.0 6.621746275411671E-4 0.0 24-25 5.75804023948841E-5 0.0 0.0 0.0011228178467002398 0.0 26-27 5.75804023948841E-5 0.0 0.0 0.001813782675438849 0.0 28-29 1.4395100598721023E-4 0.0 0.0 0.004937519505361311 0.0 30-31 1.4395100598721023E-4 0.0 0.0 0.012581317923282175 0.0 32-33 1.4395100598721023E-4 0.0 0.0 0.027652988250143087 0.0 34-35 1.4395100598721023E-4 0.0 0.0 0.050670754107498 0.0 36-37 1.4395100598721023E-4 0.0 0.0 0.08906248740428697 0.0 38-39 1.4395100598721023E-4 0.0 0.0 0.14763615174048283 0.0 40-41 1.4395100598721023E-4 0.0 0.0 0.2133497859736443 0.0 42-43 1.4395100598721023E-4 0.0 0.0 0.27899144470381215 0.0 44-45 1.4395100598721023E-4 0.0 0.0 0.36182085354885296 0.0 46-47 2.0153140838209433E-4 0.0 0.0 0.46245700183451166 0.0 48-49 2.0153140838209433E-4 0.0 0.0 0.5728674234267019 0.0 50-51 2.0153140838209433E-4 0.0 0.0 0.6963773865637282 0.0 52-53 2.0153140838209433E-4 0.0 0.0 0.814402816372642 0.0 54-55 2.0153140838209433E-4 0.0 0.0 0.9487378951599066 0.0 56-57 2.0153140838209433E-4 0.0 0.0 1.1227746613984437 0.0 58-59 2.0153140838209433E-4 0.0 0.0 1.309148028850085 0.0 60-61 2.0153140838209433E-4 0.0 0.0 1.5178769875315397 0.0 62-63 2.0153140838209433E-4 0.0 0.0 1.7369848137446724 0.0 64-65 2.0153140838209433E-4 2.879020119744205E-5 0.0 1.9548978466081113 0.0 66-67 2.0153140838209433E-4 2.879020119744205E-5 0.0 2.209849473312059 0.0 68-69 2.0153140838209433E-4 2.879020119744205E-5 0.0 2.508317489125941 0.0 70-71 2.0153140838209433E-4 2.879020119744205E-5 0.0 2.834078615674998 0.0 72-73 2.1592650898081536E-4 2.879020119744205E-5 0.0 3.164460569516244 0.0 74-75 2.303216095795364E-4 2.879020119744205E-5 0.0 3.513743290443611 0.0 76-77 2.303216095795364E-4 2.879020119744205E-5 0.0 3.8796523525625006 0.0 78-79 2.303216095795364E-4 2.879020119744205E-5 0.0 4.315723134999557 0.0 80-81 2.303216095795364E-4 2.879020119744205E-5 0.0 4.790056094828014 0.0 82-83 2.303216095795364E-4 2.879020119744205E-5 0.0 5.264820907674431 0.0 84-85 2.5911181077697845E-4 2.879020119744205E-5 0.0 5.75421114272915 0.0 86-87 2.8790201197442046E-4 2.879020119744205E-5 0.0 6.278912559552531 0.0 88-89 2.8790201197442046E-4 2.879020119744205E-5 0.0 6.847677379308598 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14355 0.0 24.303947 1 TACATGG 14350 0.0 23.797766 2 ACATGGG 14950 0.0 21.892214 3 GAGTACT 11815 0.0 21.317371 12-13 AGTACTT 12385 0.0 19.934898 12-13 GTACTTT 12765 0.0 19.73131 14-15 CATGGGG 6170 0.0 17.579363 4 CATGGGA 9785 0.0 17.474277 4 TATCACG 245 3.8701E-6 17.399199 2 GTATAGG 885 0.0 17.163244 1 TTTGCGT 1060 0.0 16.532887 3 TACTTTT 14950 0.0 16.45165 14-15 AGTACAT 11025 0.0 16.282213 2 ACTTTTT 15930 0.0 16.170729 16-17 GTAAGCG 440 0.0 15.674601 94-95 AGAGTAC 25450 0.0 15.188496 10-11 CTTTGCG 1155 0.0 15.1730385 2 GCTTATA 1730 0.0 14.81632 1 ATGGGAG 4065 0.0 14.797761 5 GGACCGT 450 2.401066E-9 14.728826 6 >>END_MODULE