FastQCFastQC Report
Fri 27 May 2016
SRR939041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3473404
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT255520.7356472209970392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143200.4122756811473702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123540.35567414559319904No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT115650.3329586768484173No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT91970.26478348041287453No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC82850.23852681692080735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78320.2254848557783661No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT68770.19799021363480895No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC59190.17040920088765948No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC57740.1662346217140304No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT56830.16361471340506317No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55800.16064932268172663No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA51360.14786647335006234No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA50240.14464197081594884No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC45260.1303044506196227No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44890.12923921317531736No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA40220.11579418921611191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT7000.027.1179813
GTACATG141300.022.8939421
GTAAGCG4800.022.24586194-95
TACATGG142950.022.1430322
GAGTACT129250.021.42548812-13
ACATGGG146350.020.4288183
GTACTTT138100.019.89809814-15
CTTTGCG10300.019.8119242
AGTACTT133750.019.64012512-13
CATGGGG58700.017.220154
TTGCGTT11350.016.7247474
CATGGGA96100.016.6912444
ACTTTTT166450.016.65135216-17
TACTTTT157600.016.42726314-15
AGTACAT110650.015.5686382
ATGGGAG39650.015.5594985
AGAGTAC259300.015.40148710-11
CGATCGT3950.014.41715524-25
GACCGAT2651.4431778E-414.3264827
CTTACAC15400.014.1753083
Produced by FastQC (version 0.11.5)