FastQCFastQC Report
Fri 27 May 2016
SRR939040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491708
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT235430.6742545482039163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143050.40968488773975376No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT124280.3559289608409409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112000.3207599260877485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT101340.2902304545511824No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC89620.25666521942842874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73240.20975408023809552No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT64590.18498110380363994No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT55970.1602940452065293No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC54520.15614134973485755No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC52090.149182004909918No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50210.14379782043630224No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG48410.1386427501956063No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA46930.1344041368865896No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC46160.13219891239473633No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42660.12217516470449417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG145050.026.0483861
TACATGG145150.025.3661822
ACATGGG148900.023.6788353
TTTGCGT8550.022.6942843
GAGTACT120600.021.58388912-13
GTACCGT2309.39508E-820.5755886
CATGGGG61450.020.4090484
CATGGGA97300.019.5529374
ACCGTCC3655.456968E-1219.4487178
GTACTTT132550.019.44217714-15
AGTACTT126700.019.06924212-13
TATGCCG1250.006162876718.9303555
CTTTGCG10850.017.8832592
TTGCGTT11350.017.0956954
GTGTAGC19150.017.0812851
AGTACAT108250.016.6567252
ACTTTTT161900.016.44463516-17
GTATTAG9100.016.1495531
GTACACG5103.6379788E-1215.8022531
TACTTTT152850.015.71438314-15
Produced by FastQC (version 0.11.5)