##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939040_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3491708 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68634633823905 33.0 31.0 34.0 30.0 34.0 2 31.734570588376805 33.0 31.0 34.0 30.0 34.0 3 31.787035742965905 33.0 31.0 34.0 30.0 34.0 4 35.32320428856021 37.0 35.0 37.0 33.0 37.0 5 35.36480627818821 37.0 35.0 37.0 33.0 37.0 6 35.451480765287364 37.0 35.0 37.0 33.0 37.0 7 35.35617325389179 37.0 35.0 37.0 33.0 37.0 8 35.3421417254822 37.0 35.0 37.0 33.0 37.0 9 36.919747584849596 39.0 37.0 39.0 33.0 39.0 10-11 36.80201422913943 39.0 37.0 39.0 32.0 39.0 12-13 36.672717478093816 39.0 37.0 39.0 32.0 39.0 14-15 37.83632637666151 40.0 38.0 41.0 32.0 41.0 16-17 37.77031040396276 40.0 38.0 41.0 32.0 41.0 18-19 37.716669034180406 40.0 37.5 41.0 32.0 41.0 20-21 37.58687267091062 40.0 37.0 41.0 32.0 41.0 22-23 37.6805196769031 40.0 37.5 41.0 32.0 41.0 24-25 37.60908687095255 40.0 37.0 41.0 32.0 41.0 26-27 37.51347034173533 40.0 37.0 41.0 32.0 41.0 28-29 37.38846046118404 40.0 37.0 41.0 31.0 41.0 30-31 37.209985199220554 40.0 37.0 41.0 31.0 41.0 32-33 37.00978446651324 40.0 36.0 41.0 30.5 41.0 34-35 36.672343162715784 39.5 36.0 41.0 30.0 41.0 36-37 36.396017794156904 39.0 35.5 41.0 30.0 41.0 38-39 36.18219980021239 39.0 35.0 41.0 29.5 41.0 40-41 36.04146552346302 39.0 35.0 41.0 29.0 41.0 42-43 35.83271596593988 39.0 35.0 41.0 27.5 41.0 44-45 35.745003734562 39.0 35.0 41.0 27.0 41.0 46-47 35.50781694803804 39.0 35.0 41.0 26.0 41.0 48-49 35.308976724285074 39.0 35.0 41.0 25.5 41.0 50-51 34.133011551939624 38.0 34.0 40.0 23.5 40.5 52-53 34.290642287384856 38.0 33.5 39.5 24.0 40.5 54-55 34.791896687810095 39.0 34.0 40.0 24.0 41.0 56-57 34.68192815664999 38.5 34.0 40.0 23.5 41.0 58-59 34.47866072993504 38.0 34.0 40.0 23.0 41.0 60-61 34.180453949757535 38.0 34.0 40.0 22.5 41.0 62-63 33.82836766419185 37.5 33.5 40.0 22.0 41.0 64-65 33.412291491728396 37.0 33.0 40.0 20.5 41.0 66-67 33.00823207438881 36.0 32.5 39.0 19.5 41.0 68-69 32.60290766009071 36.0 32.0 39.0 18.5 41.0 70-71 32.16098038553052 35.0 32.0 38.5 16.5 40.0 72-73 31.710260852282033 35.0 31.0 37.5 13.0 40.0 74-75 31.241967827779412 35.0 31.0 37.0 9.0 39.0 76-77 30.822156234140998 35.0 31.0 36.5 7.0 39.0 78-79 30.38027306407065 35.0 30.0 36.0 4.5 38.5 80-81 29.92631700588938 34.0 30.0 35.5 2.0 37.5 82-83 29.52929053632205 34.0 29.5 35.0 2.0 37.0 84-85 29.158295596309884 34.0 29.0 35.0 2.0 36.5 86-87 28.76354423107545 34.0 29.0 35.0 2.0 36.0 88-89 28.321171329332238 34.0 28.5 35.0 2.0 36.0 90-91 27.866165927964197 34.0 27.0 35.0 2.0 35.0 92-93 27.418595999436377 34.0 26.5 35.0 2.0 35.0 94-95 26.911727297929836 33.0 25.5 35.0 2.0 35.0 96-97 26.284383459327067 33.0 25.0 35.0 2.0 35.0 98-99 25.546967844962982 33.0 22.0 35.0 2.0 35.0 100-101 22.86689035852941 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3656742402245854 1101 2 0.3837812215588734 1101 3 0.32786733976146465 1101 4 0.3636642292355532 1101 5 0.21429429094671804 1101 6 0.34814777842847633 1101 7 0.2930675973160817 1101 8 0.36143998136239475 1101 9 0.40658556732190476 1101 10-11 0.4752472440666722 1101 12-13 0.606768558617425 1101 14-15 0.42266638002104884 1101 16-17 0.591003666197885 1101 18-19 0.7299947008683176 1101 20-21 0.7160037101474543 1101 22-23 0.702753851652588 1101 24-25 0.7006254750380165 1101 26-27 0.7347252322710602 1101 28-29 0.738913400684055 1101 30-31 0.7509482620996621 1101 32-33 0.7822258467087551 1101 34-35 0.6689475952732593 1101 36-37 0.58207829811208 1101 38-39 0.7375744926975045 1101 40-41 0.7462841913970024 1101 42-43 0.5727390322050212 1101 44-45 0.701131038815241 1101 46-47 0.699824607685116 1101 48-49 0.8039687543705796 1101 50-51 0.7076296879449941 1101 52-53 0.6380405358196768 1101 54-55 0.6808223494739991 1101 56-57 0.6284548132361536 1101 58-59 0.5951748450863761 1101 60-61 0.6548630857822744 1101 62-63 0.6729734098109788 1101 64-65 0.7579078392279541 1101 66-67 0.7121909830222748 1101 68-69 0.6579182899478866 1101 70-71 0.5702367118262686 1101 72-73 0.5243604334518253 1101 74-75 0.578038712947297 1101 76-77 0.5746330235577872 1101 78-79 0.5198207095575889 1101 80-81 0.35508349369726844 1101 82-83 0.4808978937938093 1101 84-85 0.36562054833668967 1101 86-87 0.31781781656416896 1101 88-89 0.36446526676023794 1101 90-91 0.4394714492585443 1101 92-93 0.5311857189439841 1101 94-95 0.5835812985445834 1101 96-97 0.4284280728590488 1101 98-99 0.2856712864118869 1101 100-101 0.5653645081152945 1102 1 0.16555815836271748 1102 2 0.12730078162199732 1102 3 0.0658739403381432 1102 4 0.13033643555359475 1102 5 0.19007864473555003 1102 6 0.17140738423506008 1102 7 0.20637620256222533 1102 8 0.11610836076911824 1102 9 0.2633111391546592 1102 10-11 0.13097107104858452 1102 12-13 0.21315643311636023 1102 14-15 0.4202571890426938 1102 16-17 0.30863342246777137 1102 18-19 0.3539351264231527 1102 20-21 0.5000438382796801 1102 22-23 0.4098602520679364 1102 24-25 0.4745796908876727 1102 26-27 0.35413966356143334 1102 28-29 0.35378692802780876 1102 30-31 0.43379507686668717 1102 32-33 0.3812700292526827 1102 34-35 0.3562319680591557 1102 36-37 0.4040684443449436 1102 38-39 0.44321091921548117 1102 40-41 0.3952034438097414 1102 42-43 0.3255910314540529 1102 44-45 0.5198574509781224 1102 46-47 0.36950561573197405 1102 48-49 0.32126667963953537 1102 50-51 0.19929420021677657 1102 52-53 0.2003292398450256 1102 54-55 0.3255256918531586 1102 56-57 0.37451779786469075 1102 58-59 0.2754761451456318 1102 60-61 0.23693540526527102 1102 62-63 0.20407263797249442 1102 64-65 0.32634862182555224 1102 66-67 0.322131960188905 1102 68-69 0.3070682940770766 1102 70-71 0.36500396146924885 1102 72-73 0.3042398313087489 1102 74-75 0.18596572183189153 1102 76-77 0.20102133094842856 1102 78-79 0.10421364443051928 1102 80-81 0.019850669953029865 1102 82-83 -0.024909125498762563 1102 84-85 -0.01916416282003297 1102 86-87 -0.06284206831821848 1102 88-89 -5.698285878779075E-4 1102 90-91 0.06912790324192386 1102 92-93 -0.13481896093120938 1102 94-95 -0.13446072669374942 1102 96-97 0.02124734698140429 1102 98-99 -0.23891501171096863 1102 100-101 -0.02409437446487317 1103 1 0.03423224849385775 1103 2 0.20562276331476426 1103 3 0.2959730706909873 1103 4 0.3173109003098631 1103 5 0.3084877077622252 1103 6 0.31548118412618464 1103 7 0.30615831263791904 1103 8 0.20673284327472174 1103 9 0.3697606529256703 1103 10-11 0.3975183670630784 1103 12-13 0.4198165131725915 1103 14-15 0.4322934732428081 1103 16-17 0.5274838630289835 1103 18-19 0.47390277179856355 1103 20-21 0.5556501772858056 1103 22-23 0.5267551410541742 1103 24-25 0.6049798368006876 1103 26-27 0.4805746420080155 1103 28-29 0.49819528294721493 1103 30-31 0.5158406858785938 1103 32-33 0.5765021536258033 1103 34-35 0.4394265487528273 1103 36-37 0.4190676769955033 1103 38-39 0.48906898149253664 1103 40-41 0.4998111323246448 1103 42-43 0.42184319414333515 1103 44-45 0.5350669700296109 1103 46-47 0.46438382641301956 1103 48-49 0.3710518451187923 1103 50-51 0.2541770849655549 1103 52-53 0.3075152691405094 1103 54-55 0.45034214606432243 1103 56-57 0.4828583498804946 1103 58-59 0.40611633848970996 1103 60-61 0.4026012056900399 1103 62-63 0.43906741113326575 1103 64-65 0.23747444241132598 1103 66-67 0.41672888715262957 1103 68-69 0.29041811726017386 1103 70-71 0.29864992556102976 1103 72-73 0.44863078675443546 1103 74-75 0.34357057465240004 1103 76-77 0.3994569643242585 1103 78-79 0.3080480126360321 1103 80-81 0.1517412463887311 1103 82-83 0.29900740740653475 1103 84-85 0.2799746798490723 1103 86-87 0.18926151872556218 1103 88-89 0.23590469284496152 1103 90-91 0.2868674830329745 1103 92-93 0.28690957649142135 1103 94-95 0.33208582296262534 1103 96-97 0.2844936876108548 1103 98-99 0.28714949322269234 1103 100-101 0.4956414334188146 1104 1 0.12173212823563873 1104 2 0.1089332315521645 1104 3 0.1772246540908604 1104 4 0.24385525635115357 1104 5 0.20308121894944264 1104 6 0.3040018156261368 1104 7 0.16194273294520656 1104 8 0.29178009673735517 1104 9 0.3368541560956757 1104 10-11 0.18894988882661323 1104 12-13 0.26399296885824697 1104 14-15 0.36435527634642995 1104 16-17 0.23342590177457367 1104 18-19 0.2621973178478356 1104 20-21 0.29987024661868134 1104 22-23 0.22117163214383595 1104 24-25 0.3394442387645782 1104 26-27 0.33823284570298284 1104 28-29 0.3736359892317509 1104 30-31 0.4114466109208408 1104 32-33 0.44142170889487176 1104 34-35 0.36237021338953923 1104 36-37 0.4137506602972465 1104 38-39 0.3457798711147291 1104 40-41 0.4185534558416961 1104 42-43 0.39722329028576553 1104 44-45 0.4640989074055497 1104 46-47 0.6083120691357848 1104 48-49 0.5453547483125263 1104 50-51 0.638267724386516 1104 52-53 0.6130356024000818 1104 54-55 0.5742844349470104 1104 56-57 0.6028058210906764 1104 58-59 0.6082415417260947 1104 60-61 0.46180448697561616 1104 62-63 0.48528601171243224 1104 64-65 0.4707215105159577 1104 66-67 0.5807787349859055 1104 68-69 0.48442540971853987 1104 70-71 0.5399921355543214 1104 72-73 0.5319655631863895 1104 74-75 0.5943413496604997 1104 76-77 0.5336811705398432 1104 78-79 0.44076337907084095 1104 80-81 0.40303281965485027 1104 82-83 0.25343627707548677 1104 84-85 0.20954056371490992 1104 86-87 0.2908880512161254 1104 88-89 0.441049820462986 1104 90-91 0.3574122367281056 1104 92-93 0.37825194682900687 1104 94-95 0.33148068360739913 1104 96-97 0.2890121897386244 1104 98-99 0.30241170424219277 1104 100-101 0.6736829241076236 1105 1 0.10414842401930002 1105 2 0.14368974084761632 1105 3 0.18016493256065402 1105 4 0.11499718071846132 1105 5 0.16354065073514334 1105 6 0.2382983975435664 1105 7 0.18644110058798447 1105 8 0.174427373944269 1105 9 0.15909456970286584 1105 10-11 0.12067447932133035 1105 12-13 0.2802831771043657 1105 14-15 0.3053718689544951 1105 16-17 0.23212178596880761 1105 18-19 0.1120634194212542 1105 20-21 0.29940823952286166 1105 22-23 0.3056672917285468 1105 24-25 0.25868994724478256 1105 26-27 0.20591593388036955 1105 28-29 0.34476937348129866 1105 30-31 0.3509120437986084 1105 32-33 0.1566682528021417 1105 34-35 0.2670122762571978 1105 36-37 0.36148814858223943 1105 38-39 0.25457172322290234 1105 40-41 0.2478791582331965 1105 42-43 0.19152371304372195 1105 44-45 0.10833521290636128 1105 46-47 0.25434631279552633 1105 48-49 0.25041001128178664 1105 50-51 0.22298175433976297 1105 52-53 0.1705613931132106 1105 54-55 0.21017841712869512 1105 56-57 0.126029321724765 1105 58-59 0.10922591198312404 1105 60-61 0.2964454170608164 1105 62-63 0.2002469705419827 1105 64-65 0.14913465928835024 1105 66-67 0.15040932400622609 1105 68-69 0.18791022025625637 1105 70-71 0.04627558250515307 1105 72-73 0.24606454536365163 1105 74-75 0.2100029521405844 1105 76-77 0.08413928369596135 1105 78-79 -0.07263726042025453 1105 80-81 0.025013135789652807 1105 82-83 -0.005697976230599977 1105 84-85 -0.0046527137521898965 1105 86-87 0.057792926694130387 1105 88-89 -0.03138188293208799 1105 90-91 0.036192876059907064 1105 92-93 0.10499884340331533 1105 94-95 -0.027802866886819544 1105 96-97 0.004151871550654107 1105 98-99 0.0040907887415677635 1105 100-101 0.3162930672895534 1106 1 0.30288215495915694 1106 2 0.28020467528747517 1106 3 0.23883361318329932 1106 4 0.23079573212186233 1106 5 0.19817545127227731 1106 6 0.21316173830250307 1106 7 0.2772656185433533 1106 8 0.2531392947086957 1106 9 0.27713929266006687 1106 10-11 0.3395607605135993 1106 12-13 0.35307169962916873 1106 14-15 0.3616282093729666 1106 16-17 0.4497148378206859 1106 18-19 0.342817581414522 1106 20-21 0.47080597451366657 1106 22-23 0.43057038161861527 1106 24-25 0.4736386449422767 1106 26-27 0.448915852653883 1106 28-29 0.43299533519424216 1106 30-31 0.406554000907569 1106 32-33 0.5430327454217618 1106 34-35 0.5060912024776272 1106 36-37 0.4021949745449973 1106 38-39 0.47084259799758854 1106 40-41 0.5168873655646635 1106 42-43 0.5892937003758476 1106 44-45 0.6561264573825198 1106 46-47 0.6774300158245836 1106 48-49 0.6820232729745115 1106 50-51 0.6255212917635689 1106 52-53 0.47416162746026913 1106 54-55 0.3586377926525657 1106 56-57 0.5505827056719923 1106 58-59 0.6413225576749255 1106 60-61 0.6403038306557107 1106 62-63 0.5410586132353146 1106 64-65 0.3730123944174153 1106 66-67 0.491910700535918 1106 68-69 0.5685110024500233 1106 70-71 0.5973286752467786 1106 72-73 0.46709693399263585 1106 74-75 0.4953254126463662 1106 76-77 0.4258931536195192 1106 78-79 0.5035614783225348 1106 80-81 0.41624784749611976 1106 82-83 0.40950744900733227 1106 84-85 0.46451094724648456 1106 86-87 0.5251119687746346 1106 88-89 0.6792100507594512 1106 90-91 0.6651095048496849 1106 92-93 0.5603085496226718 1106 94-95 0.6092371881398044 1106 96-97 0.7634405522078609 1106 98-99 0.7320913728809764 1106 100-101 1.0877565611690514 1107 1 0.008501931596509138 1107 2 -0.11048294598466413 1107 3 0.07848808987210987 1107 4 0.0451180328459273 1107 5 0.016269533547763615 1107 6 -0.04943116712293261 1107 7 -0.14265369091145885 1107 8 0.016214832320969208 1107 9 0.07092948165740864 1107 10-11 0.029863021414158197 1107 12-13 0.11157586849385126 1107 14-15 0.023206231973503577 1107 16-17 0.0786922335584066 1107 18-19 0.07524089304041581 1107 20-21 0.02811782673369123 1107 22-23 0.010657340114278213 1107 24-25 0.0898195115660485 1107 26-27 0.08036781830009687 1107 28-29 0.06709076660371949 1107 30-31 0.11999592563592643 1107 32-33 0.12856663758523723 1107 34-35 -0.0024585360810789325 1107 36-37 -0.08163134156367846 1107 38-39 0.08113260759601815 1107 40-41 0.149541845980373 1107 42-43 0.15622914353519235 1107 44-45 0.30714188230043504 1107 46-47 0.32462300737504535 1107 48-49 0.30035218316765366 1107 50-51 0.4535473650823221 1107 52-53 0.23194472791382736 1107 54-55 0.3047421428473598 1107 56-57 0.3744218727731763 1107 58-59 0.3850264861213546 1107 60-61 0.3191924078411219 1107 62-63 0.288105138029465 1107 64-65 0.48257769341729784 1107 66-67 0.31558834085924303 1107 68-69 0.4042098941747412 1107 70-71 0.519449983466302 1107 72-73 0.40923951597488895 1107 74-75 0.4272087149279322 1107 76-77 0.5257460939489711 1107 78-79 0.44082132688206954 1107 80-81 0.32853262386411686 1107 82-83 0.3773710739900977 1107 84-85 0.4427701375018209 1107 86-87 0.4829611029335048 1107 88-89 0.4803243398908563 1107 90-91 0.7006215947097338 1107 92-93 0.47676422352391157 1107 94-95 0.5303091228663845 1107 96-97 0.6684780531861705 1107 98-99 0.6816416559043503 1107 100-101 0.5776913338270973 1108 1 0.23233390610442228 1108 2 0.192403147110074 1108 3 0.2002145361746308 1108 4 0.18230574177076164 1108 5 0.2537705103392014 1108 6 0.23366400275195076 1108 7 0.2743803361608599 1108 8 0.20880267843073597 1108 9 0.2959261388600467 1108 10-11 0.3510704224543346 1108 12-13 0.29516603285529186 1108 14-15 0.37652072408537407 1108 16-17 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-2.039480223408134 2316 64-65 -2.463298033540106 2316 66-67 -2.537945145703052 2316 68-69 -2.6630610126087504 2316 70-71 -2.549901626695089 2316 72-73 -2.4819350242125076 2316 74-75 -2.4361828085266595 2316 76-77 -2.5229590040152985 2316 78-79 -2.44681482308113 2316 80-81 -2.400299064018224 2316 82-83 -2.576020038730423 2316 84-85 -2.656873529733172 2316 86-87 -2.615060583825599 2316 88-89 -2.8955032054449674 2316 90-91 -3.046158962648022 2316 92-93 -3.2935901645409977 2316 94-95 -3.649103502260818 2316 96-97 -3.734469047618031 2316 98-99 -4.022451895905984 2316 100-101 -4.512994139430759 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11415.0 3 3210.0 4 3165.0 5 3687.0 6 4203.0 7 5273.0 8 6670.0 9 8216.0 10 10003.0 11 12853.0 12 16385.0 13 22936.0 14 22408.0 15 21163.0 16 25950.0 17 33075.0 18 29412.0 19 22181.0 20 22452.0 21 24243.0 22 26705.0 23 29665.0 24 33580.0 25 38265.0 26 44290.0 27 51765.0 28 59903.0 29 71349.0 30 84937.0 31 102405.0 32 126322.0 33 159262.0 34 211957.0 35 300646.0 36 452560.0 37 683900.0 38 608796.0 39 96476.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.09251198995483 18.594417429929237 13.593373451587695 22.71969712852824 2 15.20741554166871 20.913625324012884 39.69503590620273 24.183923228115674 3 20.513356334412297 24.49878630896893 29.774873786452293 25.212983570166475 4 12.602202549333452 16.086585939589725 37.75835717904192 33.5528543320349 5 13.36162656149835 37.91948155903849 34.24765968768313 14.471232191780036 6 31.59053327910282 35.87910035825959 17.42470429037285 15.105662072264733 7 27.956797217354612 33.20590434971803 20.97684979614325 17.860448636784113 8 25.68968388670383 34.36688336351266 20.01303678189848 19.93039596788503 9 26.02858710490061 17.406733007952585 21.183249981510286 35.38142990563652 10-11 25.06713179272271 26.126163054009275 27.314255330126752 21.492449823141264 12-13 26.67141845454716 23.844209772563456 27.519573892762 21.964797880127385 14-15 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26.762273043702166 29.435719513280468 21.654883281264087 40-41 22.172058000857408 26.915484851547205 29.10846844419031 21.80398870340507 42-43 21.737575912479706 27.102996180526805 28.50611439700202 22.65331350999147 44-45 22.17812473265217 26.643053161927305 28.498321091623147 22.68050101379738 46-47 22.639693282570907 27.126845537163767 28.19355352498804 22.039907655277283 48-49 22.535663073531435 26.97274215491606 28.285961421150162 22.205633350402344 50-51 21.790652455710788 27.38679245283019 28.805185078496326 22.017370012962697 52-53 22.371686458399047 27.737260971729267 27.743281599147778 22.147770970723908 54-55 21.808916881892525 27.03283842787213 28.236399005052366 22.92184568518298 56-57 22.422682119348323 27.180048774602188 27.89639154907595 22.500877556973535 58-59 22.97770909947941 26.91349773796894 27.75550916107104 22.353284001480617 60-61 22.357582780613587 26.920882496287824 28.83619258749971 21.885342135598883 62-63 22.11215043443011 27.05031785724829 28.554489351116153 22.28304235720545 64-65 21.845526614208076 27.69183054214896 28.223071754873626 22.239571088769345 66-67 22.056358174136694 27.122810728073567 27.779513065467665 23.04131803232207 68-69 21.687377975784404 27.259930615397536 28.214123782027055 22.838567626791004 70-71 22.42698458435384 27.0998703356865 27.78282668203429 22.69031839792537 72-73 22.279037223661923 26.61149040866897 28.397107712263093 22.712364655406013 74-75 21.742089797861034 27.877248425421552 27.781207855303293 22.599453921414124 76-77 22.193280268978206 26.852624277403365 27.8232543321231 23.130841121495326 78-79 22.108035596775313 27.01077113194946 28.065925052781456 22.81526821849377 80-81 22.396773611089912 27.06892664022838 27.849447471251885 22.68485227742982 82-83 22.203420999992368 26.931161940975457 28.206379240301654 22.65903781873052 84-85 22.066795634600584 27.018513312348198 27.968201991897836 22.946489061153382 86-87 22.01775210560537 27.07951436967208 28.050112579772506 22.85262094495004 88-89 22.12537015059512 26.95272442360207 27.57005496088214 23.35185046492067 90-91 22.174865332245282 26.493006063740214 27.88300633023076 23.44912227378374 92-93 22.493695495115222 26.90728830297351 27.89715139383401 22.701864808077257 94-95 22.324013947385556 26.684343881077275 27.882886101537473 23.108756069999696 96-97 21.659060592178996 26.572210169667322 28.438377831750174 23.33035140640351 98-99 22.154068672328737 26.838233440394493 27.470213634919755 23.537484252357014 100-101 22.305160762534637 25.925212727346047 28.15575328812817 23.61387322199114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4758.0 1 2845.0 2 1136.0 3 1329.5 4 1120.0 5 1116.0 6 1876.0 7 3496.5 8 5391.5 9 6163.0 10 5417.0 11 4731.0 12 4901.0 13 5233.5 14 5637.5 15 6115.5 16 6629.0 17 6884.5 18 7033.0 19 7308.0 20 7787.5 21 8746.0 22 10094.5 23 11882.0 24 14096.0 25 16895.5 26 20853.5 27 25491.0 28 29679.0 29 34150.5 30 39083.5 31 44329.5 32 49442.5 33 54580.0 34 61314.5 35 67567.5 36 74551.0 37 82074.0 38 86862.0 39 89983.0 40 93031.0 41 97343.0 42 101574.5 43 106589.0 44 113598.0 45 118261.5 46 123441.0 47 143171.0 48 187178.5 49 187380.0 50 146065.0 51 128323.5 52 120483.0 53 116837.0 54 113066.5 55 105612.0 56 96497.0 57 86695.5 58 77874.0 59 68659.0 60 57723.5 61 46400.0 62 36619.5 63 28417.0 64 21051.0 65 15412.0 66 11161.5 67 8520.5 68 6284.5 69 4005.5 70 2702.0 71 1823.5 72 1224.5 73 829.5 74 540.0 75 333.5 76 216.5 77 135.5 78 92.0 79 65.0 80 49.5 81 34.5 82 25.0 83 24.0 84 19.0 85 17.5 86 16.0 87 18.5 88 18.0 89 9.0 90 7.5 91 7.0 92 5.0 93 4.0 94 3.0 95 3.5 96 3.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2816673100958041 2 0.08692021211395684 3 0.09095835046916867 4 0.10479112228170281 5 0.10630900407479663 6 0.08296799159608995 7 0.09247623226226248 8 0.089268633001385 9 0.09399411405535629 10-11 0.08729252274245154 12-13 0.0915454556910257 14-15 0.10000836266950157 16-17 0.08906815804758014 18-19 0.09950717528498948 20-21 0.10149760518348042 22-23 0.11341154529531106 24-25 0.12518228901156683 26-27 0.12836124899332932 28-29 0.12144486308706226 30-31 0.11395569159849564 32-33 0.10845695000841994 34-35 0.3801148320535394 36-37 0.5823511015239533 38-39 0.565024337659392 40-41 0.5283660603922207 42-43 0.5547571560966724 44-45 0.5758499851648534 46-47 0.5646949859495697 48-49 0.587262737892172 50-51 0.5787425523554661 52-53 0.5816351195460789 54-55 0.571854805728314 56-57 0.5441176639054583 58-59 0.5393778632119295 60-61 0.48676750747771574 62-63 0.5622033686665666 64-65 0.5951242200092333 66-67 0.6089283525426524 68-69 0.6112051752322931 70-71 0.6073818314704437 72-73 0.5983174996305533 74-75 0.5950955807301184 76-77 0.5785134381225463 78-79 0.5547714757362299 80-81 0.5504183053107533 82-83 0.5645231502748798 84-85 0.5740457105806098 86-87 0.5649241001824895 88-89 0.5770241956085674 90-91 0.5918450225505684 92-93 0.6007231990762114 94-95 0.5999928974587795 96-97 0.5956397270333029 98-99 0.6000072170983369 100-101 0.576451410026268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3491708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.47269682227015 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18091213801904 34.50330773598846 2 14.480216533700446 14.037902919177705 3 5.733144756100798 8.337029628019858 4 2.7731548317231516 5.376891735973197 5 1.6041417260395379 3.8878537773133823 6 0.974786480237024 2.8350317713786257 7 0.6937907393673733 2.3540935717217333 8 0.48497280283679983 1.8806351711163838 9 0.34503365811725567 1.505224071305689 >10 1.6060852368564853 13.361605175809224 >50 0.08213238139660142 2.7270364268969156 >100 0.03707082189964509 3.1451210088286143 >500 0.0020125764450891434 0.664406988330834 >1k 0.0018349961647957584 2.06601421136397 >5k 4.143539726958164E-4 1.263165191876778 >10k+ 2.959671233541546E-4 2.0546806148986025 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23543 0.6742545482039163 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14305 0.40968488773975376 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12428 0.3559289608409409 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11200 0.3207599260877485 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10134 0.2902304545511824 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8962 0.25666521942842874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7324 0.20975408023809552 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6459 0.18498110380363994 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5597 0.1602940452065293 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5452 0.15614134973485755 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5209 0.149182004909918 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5021 0.14379782043630224 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4841 0.1386427501956063 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4693 0.1344041368865896 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4616 0.13219891239473633 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4266 0.12217516470449417 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.863927911497754E-5 0.0 10-11 0.0 0.0 0.0 5.727855822995508E-5 0.0 12-13 0.0 0.0 0.0 5.727855822995508E-5 0.0 14-15 0.0 0.0 0.0 7.159819778744385E-5 0.0 16-17 0.0 0.0 0.0 1.1455711645991016E-4 0.0 18-19 0.0 2.863927911497754E-5 0.0 1.2887675601739893E-4 0.0 20-21 0.0 2.863927911497754E-5 0.0 3.436713493797305E-4 0.0 22-23 2.863927911497754E-5 2.863927911497754E-5 0.0 6.014248614145284E-4 0.0 24-25 5.727855822995508E-5 2.863927911497754E-5 0.0 0.0010882926063691466 0.0 26-27 5.727855822995508E-5 2.863927911497754E-5 0.0 0.0017040371073411638 0.0 28-29 5.727855822995508E-5 2.863927911497754E-5 0.0 0.00442476862326403 0.0 30-31 5.727855822995508E-5 2.863927911497754E-5 0.0 0.013532059381826889 0.0 32-33 5.727855822995508E-5 2.863927911497754E-5 0.0 0.030328996582761218 0.0 34-35 5.727855822995508E-5 2.863927911497754E-5 0.0 0.055144931935889256 0.0 36-37 8.591783734493262E-5 2.863927911497754E-5 0.0 0.09618501890765208 0.0 38-39 8.591783734493262E-5 2.863927911497754E-5 0.0 0.15629886576998991 0.0 40-41 8.591783734493262E-5 2.863927911497754E-5 0.0 0.22425987510983164 0.0 42-43 8.591783734493262E-5 2.863927911497754E-5 0.0 0.2953425658732059 0.0 44-45 8.591783734493262E-5 2.863927911497754E-5 0.0 0.3836088241055666 0.0 46-47 1.2887675601739893E-4 2.863927911497754E-5 0.0 0.4902471798901855 0.0 48-49 1.431963955748877E-4 2.863927911497754E-5 0.0 0.6047040588731933 0.0 50-51 1.431963955748877E-4 2.863927911497754E-5 0.0 0.7304877727461747 0.0 52-53 1.431963955748877E-4 2.863927911497754E-5 0.0 0.8555984635599541 0.0 54-55 1.431963955748877E-4 2.863927911497754E-5 0.0 0.9950431135707797 0.0 56-57 1.431963955748877E-4 2.863927911497754E-5 0.0 1.1722200138155883 0.0 58-59 1.431963955748877E-4 2.863927911497754E-5 0.0 1.3641891017232828 0.0 60-61 1.431963955748877E-4 2.863927911497754E-5 0.0 1.5778094846419002 0.0 62-63 1.431963955748877E-4 2.863927911497754E-5 0.0 1.7957543987068794 0.0 64-65 1.431963955748877E-4 2.863927911497754E-5 0.0 2.0145155322266355 0.0 66-67 1.431963955748877E-4 2.863927911497754E-5 0.0 2.272240404982318 0.0 68-69 1.431963955748877E-4 2.863927911497754E-5 0.0 2.5760172385548845 0.0 70-71 1.431963955748877E-4 2.863927911497754E-5 0.0 2.906700674855973 0.0 72-73 1.431963955748877E-4 2.863927911497754E-5 0.0 3.2421382314901477 0.0 74-75 1.431963955748877E-4 2.863927911497754E-5 0.0 3.5924252543454376 0.0 76-77 1.431963955748877E-4 2.863927911497754E-5 0.0 3.9645354078863413 0.0 78-79 1.431963955748877E-4 5.727855822995508E-5 0.0 4.4064108453513295 0.0 80-81 1.7183567468986524E-4 8.591783734493262E-5 0.0 4.8942380061563 0.0 82-83 1.7183567468986524E-4 8.591783734493262E-5 0.0 5.3709960855833305 0.0 84-85 2.1479459336233155E-4 8.591783734493262E-5 0.0 5.8655964358989925 0.0 86-87 2.5775351203479786E-4 8.591783734493262E-5 0.0 6.388406476142908 0.0 88-89 2.5775351203479786E-4 1.1455711645991016E-4 0.0 6.960905665651309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14505 0.0 26.048386 1 TACATGG 14515 0.0 25.366182 2 ACATGGG 14890 0.0 23.678835 3 TTTGCGT 855 0.0 22.694284 3 GAGTACT 12060 0.0 21.583889 12-13 GTACCGT 230 9.39508E-8 20.575588 6 CATGGGG 6145 0.0 20.409048 4 CATGGGA 9730 0.0 19.552937 4 ACCGTCC 365 5.456968E-12 19.448717 8 GTACTTT 13255 0.0 19.442177 14-15 AGTACTT 12670 0.0 19.069242 12-13 TATGCCG 125 0.0061628767 18.930355 5 CTTTGCG 1085 0.0 17.883259 2 TTGCGTT 1135 0.0 17.095695 4 GTGTAGC 1915 0.0 17.081285 1 AGTACAT 10825 0.0 16.656725 2 ACTTTTT 16190 0.0 16.444635 16-17 GTATTAG 910 0.0 16.149553 1 GTACACG 510 3.6379788E-12 15.802253 1 TACTTTT 15285 0.0 15.714383 14-15 >>END_MODULE