FastQCFastQC Report
Fri 27 May 2016
SRR939040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491708
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT258200.7394661867487201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142860.4091407414365691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123750.3544110790478471No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT114490.3278911065873779No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT92960.2662307386528312No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC82850.23727642746758892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.2278827439178763No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT67520.19337241258432836No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC59020.16902902533659747No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC58980.16891446822013753No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT56640.1622128769072328No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG54570.15628454613043244No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA52070.14912472635168805No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA51320.14697678041806475No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC46530.13325856572199052No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46180.13225619095296628No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA39960.11444255934345027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT8400.027.1156043
GTACATG142050.024.5222021
TACATGG143650.023.651832
ACATGGG144300.022.229913
GAGTACT124350.021.25710312-13
CTTTGCG11150.020.42792
TTGCGTT11500.020.218814
GTACTTT132500.019.80632614-15
AGTACTT128650.019.31085812-13
CATGGGA96050.018.9216384
AGTACAT107950.017.7149432
GGACCGT3551.6079866E-917.3769026
ACTTTTT161000.016.46164716-17
CATGGGG60250.016.3031184
TACTTTT151400.016.23672914-15
AGAGTAC249200.015.50140410-11
ATGGGAG41550.015.1893075
CTATACT12050.014.9642134
TACCTGG24050.014.7980172
CTAGACT13150.014.7950174
Produced by FastQC (version 0.11.5)