##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939040_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3491708 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.150431250264916 33.0 31.0 34.0 30.0 34.0 2 32.37939942286125 34.0 31.0 34.0 30.0 34.0 3 32.48037951627112 34.0 31.0 34.0 30.0 34.0 4 36.042046184847074 37.0 35.0 37.0 35.0 37.0 5 35.96270707630764 37.0 35.0 37.0 35.0 37.0 6 36.00913621643047 37.0 35.0 37.0 35.0 37.0 7 35.952152929168186 37.0 35.0 37.0 35.0 37.0 8 35.92474112955608 37.0 35.0 37.0 35.0 37.0 9 37.600622961599306 39.0 37.0 39.0 35.0 39.0 10-11 37.52109311546097 39.0 37.0 39.0 35.0 39.0 12-13 37.451873553000425 39.0 37.0 39.0 34.0 39.0 14-15 38.69340362939856 40.0 38.0 41.0 34.0 41.0 16-17 38.69688573614975 40.0 38.0 41.0 34.0 41.0 18-19 38.81037861699775 40.0 38.0 41.0 34.0 41.0 20-21 38.80662901937963 40.0 38.0 41.0 34.0 41.0 22-23 38.7492167157162 40.0 38.0 41.0 34.0 41.0 24-25 38.684427936127534 40.0 38.0 41.0 34.0 41.0 26-27 38.519731460935446 40.0 38.0 41.0 34.0 41.0 28-29 38.34688825640632 40.0 38.0 41.0 34.0 41.0 30-31 38.1916486143744 40.0 38.0 41.0 33.5 41.0 32-33 38.02392554016544 40.0 37.0 41.0 33.0 41.0 34-35 37.978839009447526 40.0 37.0 41.0 33.0 41.0 36-37 37.8717640478528 40.0 37.0 41.0 33.0 41.0 38-39 37.74074049147294 40.0 37.0 41.0 33.0 41.0 40-41 37.601209350839184 40.0 37.0 41.0 33.0 41.0 42-43 37.4297691845939 40.0 37.0 41.0 32.0 41.0 44-45 37.179150289772224 40.0 36.5 41.0 31.0 41.0 46-47 36.86635151049286 40.0 36.0 41.0 31.0 41.0 48-49 36.56975153707011 39.5 36.0 41.0 30.0 41.0 50-51 36.29537464186581 39.0 35.5 41.0 30.0 41.0 52-53 36.07733822530406 39.0 35.0 41.0 29.0 41.0 54-55 35.84249971647114 39.0 35.0 40.0 28.5 41.0 56-57 35.556686154741456 38.5 35.0 40.0 28.0 41.0 58-59 35.25264970610372 38.0 34.5 40.0 28.0 41.0 60-61 34.81873283791199 38.0 34.0 40.0 26.5 41.0 62-63 34.51632796900543 37.0 34.0 40.0 26.0 41.0 64-65 34.26225875703237 37.0 34.0 39.5 26.0 41.0 66-67 33.799096602579596 36.0 33.0 39.0 25.5 41.0 68-69 33.37564366779811 36.0 33.0 39.0 25.0 40.0 70-71 32.89853432761274 35.0 32.0 38.5 24.0 40.0 72-73 32.37562390669552 35.0 32.0 37.5 22.5 39.5 74-75 31.91571274001148 35.0 31.0 37.0 21.5 39.0 76-77 29.941257688214478 33.0 29.0 35.0 18.5 37.5 78-79 30.91341357868413 34.0 30.5 36.0 20.0 38.0 80-81 30.90365445793291 34.0 31.0 35.5 19.5 37.0 82-83 30.581779461512816 34.0 31.0 35.0 18.0 37.0 84-85 30.1770769491607 34.0 30.0 35.0 15.0 36.5 86-87 29.62149927771738 34.0 29.5 35.0 7.0 36.0 88-89 29.044716224838965 34.0 29.0 35.0 2.0 35.5 90-91 28.571012524529543 34.0 29.0 35.0 2.0 35.0 92-93 28.21712325314717 33.0 29.0 35.0 2.0 35.0 94-95 27.819040423769685 33.0 27.5 35.0 2.0 35.0 96-97 27.18028411883239 33.0 26.5 35.0 2.0 35.0 98-99 26.294520618562608 33.0 25.0 34.5 2.0 35.0 100-101 21.98969859449874 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2042246689527687 1101 2 0.18464398090871015 1101 3 0.2136314915040245 1101 4 0.15493573785208525 1101 5 0.15223946967692115 1101 6 0.13694113982135292 1101 7 0.12034166915409372 1101 8 0.21203099127684766 1101 9 0.31730622769013905 1101 10-11 0.2729941866693295 1101 12-13 0.35334954016170883 1101 14-15 0.41377230415476873 1101 16-17 0.2905585197045255 1101 18-19 0.34623973718836254 1101 20-21 0.26196574243195414 1101 22-23 0.32505623798574845 1101 24-25 0.321747443625263 1101 26-27 0.32886187084287144 1101 28-29 0.406790591593861 1101 30-31 0.3120785966191377 1101 32-33 0.3570635534889064 1101 34-35 0.4996487559231326 1101 36-37 0.43395479774646617 1101 38-39 0.4433740851122323 1101 40-41 0.5304198368212099 1101 42-43 0.5009257971208925 1101 44-45 0.5480920102205076 1101 46-47 0.5078372876361996 1101 48-49 0.3259099597641324 1101 50-51 0.41917869811204156 1101 52-53 0.5551399458518986 1101 54-55 0.6301099586650096 1101 56-57 0.47501449570258814 1101 58-59 0.40080861721203576 1101 60-61 0.5190189000274543 1101 62-63 0.4910715974650941 1101 64-65 0.38229801448084544 1101 66-67 0.4685494119304323 1101 68-69 0.6047885157519701 1101 70-71 0.6142055273517215 1101 72-73 0.5210925669333548 1101 74-75 0.5728493460526032 1101 76-77 0.2977397991291646 1101 78-79 0.47777159971016303 1101 80-81 0.6940288404302706 1101 82-83 0.6128040091125619 1101 84-85 0.5575704082771828 1101 86-87 0.5838433140622108 1101 88-89 0.7878459119391366 1101 90-91 0.7272977028643837 1101 92-93 0.7685211400453582 1101 94-95 0.7959448749055973 1101 96-97 0.7144813223103732 1101 98-99 0.6740025961824081 1101 100-101 1.1749889538318214 1102 1 0.062131496351689464 1102 2 0.07078147864787354 1102 3 0.08060990832244386 1102 4 0.0856958241591741 1102 5 0.10302330557492212 1102 6 0.13243469999984825 1102 7 0.08384592565933247 1102 8 0.12615094333919785 1102 9 0.1607546578864003 1102 10-11 0.1271715033854406 1102 12-13 0.0821708475745524 1102 14-15 0.11560016616115121 1102 16-17 0.2108787629562272 1102 18-19 0.08418302967535851 1102 20-21 0.17806334074690966 1102 22-23 0.2629303328263788 1102 24-25 0.15278316861715524 1102 26-27 0.1304885961276554 1102 28-29 0.16114126936199824 1102 30-31 0.17724314145069542 1102 32-33 0.11255786426074366 1102 34-35 0.16937960225335758 1102 36-37 0.2294094719875588 1102 38-39 0.13197185690901136 1102 40-41 0.17549448137857127 1102 42-43 0.21275187832599585 1102 44-45 0.17891964063911558 1102 46-47 0.2355175166637835 1102 48-49 0.14103528888027483 1102 50-51 0.18699360848162883 1102 52-53 0.20729966208301676 1102 54-55 0.1557316668649733 1102 56-57 0.21992776269078007 1102 58-59 0.2696692768962663 1102 60-61 0.13762379595834062 1102 62-63 0.10093087328929329 1102 64-65 0.046033264230096904 1102 66-67 0.19827482203262292 1102 68-69 0.19278968660327678 1102 70-71 0.28561596563413616 1102 72-73 0.33287078852318785 1102 74-75 0.24926460699015607 1102 76-77 0.06803784506900357 1102 78-79 0.08536218857014788 1102 80-81 0.35070642259446316 1102 82-83 0.36025912144749483 1102 84-85 0.36633366918538357 1102 86-87 0.35010752459175265 1102 88-89 0.4241054043206631 1102 90-91 0.4529186307445343 1102 92-93 0.44645946449620766 1102 94-95 0.4748081105741626 1102 96-97 0.30486102871875786 1102 98-99 0.2744298955967892 1102 100-101 0.8037720069947305 1103 1 0.013809659265810126 1103 2 0.03414844940882489 1103 3 0.05324440788699292 1103 4 0.04646285640138359 1103 5 0.02344996119548881 1103 6 -0.0070712015937672845 1103 7 0.049764694840156665 1103 8 0.09750515883984434 1103 9 0.035935778011342734 1103 10-11 0.10110116752894527 1103 12-13 0.09410581365957427 1103 14-15 0.16291889793077274 1103 16-17 0.10844633518853186 1103 18-19 0.055947089079872114 1103 20-21 0.07543308142944483 1103 22-23 0.15793038647087343 1103 24-25 0.1278994022328419 1103 26-27 0.07614047697344262 1103 28-29 0.1751588078449302 1103 30-31 0.15880394421483146 1103 32-33 0.17700980649449605 1103 34-35 0.13047400582691893 1103 36-37 0.21429208151678125 1103 38-39 0.25310610667681743 1103 40-41 0.1529975216217352 1103 42-43 0.26334763537335704 1103 44-45 0.21764634435313468 1103 46-47 0.29108723747453524 1103 48-49 0.28206833248670904 1103 50-51 0.17028209035655806 1103 52-53 0.16254051884617127 1103 54-55 0.14512260598407067 1103 56-57 0.14864491768557286 1103 58-59 0.13362717670048596 1103 60-61 0.19275260570838526 1103 62-63 0.21848157782803668 1103 64-65 0.19591139794295032 1103 66-67 0.25945057864150556 1103 68-69 0.3044553107009307 1103 70-71 0.22922902932693745 1103 72-73 0.3116634889541601 1103 74-75 0.2547605549652623 1103 76-77 0.052894938479923326 1103 78-79 0.21348407631508692 1103 80-81 0.20757018844699004 1103 82-83 0.3019210650666686 1103 84-85 0.24289293958300107 1103 86-87 0.1813559531580502 1103 88-89 0.31540818641720847 1103 90-91 0.4315274005457681 1103 92-93 0.35159445502445763 1103 94-95 0.17994445387651226 1103 96-97 0.03249610437848105 1103 98-99 0.06830751992022854 1103 100-101 0.6304273010295098 1104 1 -0.022794376920693082 1104 2 -0.03033432801085212 1104 3 -0.013520389485925932 1104 4 0.04204440911136231 1104 5 0.009161360712532485 1104 6 -0.04183576807090361 1104 7 -0.059685676998256554 1104 8 0.032695920443160276 1104 9 0.02239566467999765 1104 10-11 0.007730441906893759 1104 12-13 0.03782015709780495 1104 14-15 0.012241443300190724 1104 16-17 -0.021355896162624788 1104 18-19 0.06512295149163805 1104 20-21 0.052894125783886636 1104 22-23 0.07750714167965356 1104 24-25 0.0024370494689875954 1104 26-27 0.014859133673112979 1104 28-29 0.026316012594968186 1104 30-31 -0.12619937491199806 1104 32-33 -0.10432932488841118 1104 34-35 0.01858191112116714 1104 36-37 0.042667248675705594 1104 38-39 0.07353621420370615 1104 40-41 -0.027570674408785578 1104 42-43 -0.029225858432532448 1104 44-45 -0.02375294820785001 1104 46-47 0.01771753805594045 1104 48-49 -0.00223588553021159 1104 50-51 -0.17060634529246954 1104 52-53 -0.1554276624326718 1104 54-55 -0.021902269829269017 1104 56-57 -0.02931062846311505 1104 58-59 -0.06532513370040505 1104 60-61 0.0024608111671469146 1104 62-63 -0.024233179298462915 1104 64-65 0.07395641620616544 1104 66-67 0.011704859032363402 1104 68-69 0.07973855889144943 1104 70-71 -0.020422863216730036 1104 72-73 0.004883170253847879 1104 74-75 -0.0472597831046393 1104 76-77 -0.15325330302423268 1104 78-79 0.14032314516372324 1104 80-81 0.15183900244440451 1104 82-83 0.2325606143067951 1104 84-85 0.12695632047445216 1104 86-87 -0.03065768252047718 1104 88-89 0.06706117571583192 1104 90-91 0.1956526101959284 1104 92-93 0.12091546108867846 1104 94-95 0.273871574189247 1104 96-97 0.28223297939651815 1104 98-99 0.17451701294364597 1104 100-101 0.960342094073134 1105 1 -0.059130852250369514 1105 2 0.011735088544234884 1105 3 -0.017439816793590524 1105 4 -0.04834950713884467 1105 5 -0.004115026702613989 1105 6 0.003659607433206702 1105 7 -0.007603790986259185 1105 8 -0.0398990629235243 1105 9 -0.08530203462112951 1105 10-11 -0.06376525573222835 1105 12-13 -0.07176340375075796 1105 14-15 -3.902315983239646E-4 1105 16-17 0.0034303132729149866 1105 18-19 0.04780098197233684 1105 20-21 0.014149823989392019 1105 22-23 -0.01763220383758579 1105 24-25 -0.05118573043226604 1105 26-27 -0.018882473338102557 1105 28-29 0.006305366209822694 1105 30-31 -0.08280677109646462 1105 32-33 -0.05651063911076193 1105 34-35 0.029196127010031603 1105 36-37 0.028003468244342855 1105 38-39 0.18523608703117134 1105 40-41 0.14304114316721694 1105 42-43 0.10442322326142772 1105 44-45 0.10294925655851017 1105 46-47 0.08124048265315054 1105 48-49 0.037210387394246425 1105 50-51 -0.01753168165363661 1105 52-53 0.10286195995252001 1105 54-55 0.1641925599922871 1105 56-57 0.18156664960223168 1105 58-59 0.0784418193183285 1105 60-61 -0.00670862034420594 1105 62-63 -0.07619563640982108 1105 64-65 -0.06318667988299609 1105 66-67 0.025041292767681966 1105 68-69 -0.12607111064581744 1105 70-71 -0.05137419693532763 1105 72-73 -0.05428139383287345 1105 74-75 -0.09672927806951392 1105 76-77 -0.3077716005583966 1105 78-79 -0.08411172471231865 1105 80-81 0.03335088648287865 1105 82-83 0.09906560872695636 1105 84-85 -0.08441154974407894 1105 86-87 -0.18000296965870533 1105 88-89 0.030062199644977028 1105 90-91 0.10593199151970367 1105 92-93 0.16465789998701297 1105 94-95 -0.10051086024875744 1105 96-97 -0.039936856553570266 1105 98-99 0.16559405011572537 1105 100-101 0.7342167233744981 1106 1 -0.02183113170404738 1106 2 -0.08410991749590835 1106 3 -0.09654629089012445 1106 4 -0.011465942713286381 1106 5 -0.0015456774304496435 1106 6 -0.02362903520309345 1106 7 -7.971843350205177E-4 1106 8 0.0378476452435379 1106 9 -0.012004982451259139 1106 10-11 0.09888326343980935 1106 12-13 0.03653500507424923 1106 14-15 0.04285111994126822 1106 16-17 -0.02762723828429614 1106 18-19 -0.06614016166201253 1106 20-21 -0.09672614316745154 1106 22-23 0.07352983608782182 1106 24-25 0.03136215430907896 1106 26-27 0.03938983931819706 1106 28-29 0.11730395079511169 1106 30-31 0.046654766434492956 1106 32-33 0.06983845504110775 1106 34-35 0.060084917582230446 1106 36-37 -0.10343697113909656 1106 38-39 -0.007398139735890652 1106 40-41 0.04997408292654626 1106 42-43 0.0874823163936469 1106 44-45 -0.004457994028655321 1106 46-47 -0.04442302539778353 1106 48-49 -0.09974437982274509 1106 50-51 0.030921472267102956 1106 52-53 0.11061122588976957 1106 54-55 0.05561504313779153 1106 56-57 -0.032048464745649596 1106 58-59 0.06787684590703691 1106 60-61 0.040026679822226185 1106 62-63 0.10916138855782265 1106 64-65 0.002089429679436705 1106 66-67 -0.020868262404974303 1106 68-69 0.019641167860790176 1106 70-71 0.06699387656254174 1106 72-73 0.19018155219990263 1106 74-75 0.28344376890877143 1106 76-77 0.24867277053257908 1106 78-79 0.2752115204067458 1106 80-81 0.3010527299784549 1106 82-83 0.3122373474403659 1106 84-85 0.1605719370842671 1106 86-87 0.15707942683369325 1106 88-89 0.3410323549603831 1106 90-91 0.5318745211889748 1106 92-93 0.37098181392841667 1106 94-95 0.483219733966191 1106 96-97 0.4150535604493797 1106 98-99 0.3517978179877126 1106 100-101 0.8509696859809353 1107 1 -0.07901008643352014 1107 2 -0.07574745513325354 1107 3 -0.07519646573206273 1107 4 -0.05310901986361927 1107 5 -0.11437183531572259 1107 6 -0.10827059144371987 1107 7 -0.12205339680566851 1107 8 -0.0994231806995387 1107 9 -0.049653850510225084 1107 10-11 -0.0644634223220848 1107 12-13 -0.024711256601506193 1107 14-15 -0.10129657939904035 1107 16-17 -0.1472582728383216 1107 18-19 -0.06093149481441884 1107 20-21 -0.0824582550296995 1107 22-23 -0.1160931992061549 1107 24-25 -0.22372462631231826 1107 26-27 -0.17014554292344997 1107 28-29 0.020301064488620568 1107 30-31 -0.15247471688649483 1107 32-33 -0.013071368124762728 1107 34-35 -0.12631351129669355 1107 36-37 -0.08141207077409263 1107 38-39 -0.13322851612123543 1107 40-41 -0.04813230147228609 1107 42-43 -0.06180671167762597 1107 44-45 -0.12383085678460048 1107 46-47 -0.2432302174813259 1107 48-49 -0.2297897835007774 1107 50-51 -0.04282621663791275 1107 52-53 -0.028702782505220625 1107 54-55 -0.06576559662005366 1107 56-57 -0.009158756618695918 1107 58-59 -0.0817669142805002 1107 60-61 -0.08407348812496451 1107 62-63 -0.1603784388465357 1107 64-65 -0.18196597154901184 1107 66-67 0.0023153617782014635 1107 68-69 0.01577900923963682 1107 70-71 -0.10623728240182118 1107 72-73 -0.015455080142110944 1107 74-75 -0.1064689134589365 1107 76-77 -0.3185044122303573 1107 78-79 -0.0975266388418845 1107 80-81 0.16506624096922096 1107 82-83 0.1483864304730922 1107 84-85 0.10658150570358771 1107 86-87 0.009782082377963519 1107 88-89 0.12452692708467339 1107 90-91 0.3921934988828646 1107 92-93 0.4515030397497952 1107 94-95 0.35085120761555544 1107 96-97 0.22651403356783462 1107 98-99 0.023018489485107096 1107 100-101 0.33651646979390293 1108 1 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7311.0 13 10173.0 14 12186.0 15 16024.0 16 21215.0 17 29636.0 18 29301.0 19 20360.0 20 18891.0 21 19915.0 22 21336.0 23 23650.0 24 27397.0 25 32247.0 26 38576.0 27 47492.0 28 57237.0 29 70270.0 30 85457.0 31 103922.0 32 129378.0 33 164221.0 34 219633.0 35 315841.0 36 498042.0 37 792144.0 38 618492.0 39 49721.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71141307442219 19.403181216136243 14.213906505119425 22.67149920432214 2 15.576302485774871 21.53395988438896 39.06884539027891 23.82089223955726 3 20.540176899099237 24.59343679368378 30.041486859725957 24.824899447491028 4 12.938223929377829 16.63042843215985 37.37649310881666 33.05485452964566 5 13.281912285605621 38.13971777611796 34.23090097190592 14.347468966370498 6 31.57660949884698 36.401841161975746 17.024103962874328 14.997445376302945 7 27.908777022591806 33.32240267513779 20.76894746066968 17.99987284160073 8 25.728497342847685 34.84455172081973 19.655881877866076 19.771069058466516 9 25.805078775201135 17.42313503878331 21.00728354146452 35.76450264455103 10-11 25.11160727071107 26.402050228713282 27.124332847993017 21.362009652582632 12-13 26.60451561241662 23.986899820947226 27.505936922560537 21.90264764407562 14-15 22.837699845303227 25.46430869244641 26.970937541482666 24.727053920767702 16-17 21.70999487487844 28.625944742912644 27.457248051730648 22.20681233047826 18-19 21.483382917471907 27.957778829157537 29.26832656109847 21.29051169227209 20-21 22.748523072376038 26.288538445941068 28.968902897951377 21.994035583731513 22-23 22.06653591881108 26.488641089117415 29.060147641211692 22.38467535085981 24-25 22.56537488243576 26.484502713285302 29.152208603926788 21.79791380035215 26-27 22.292156159678875 26.23087325744306 29.805026651713145 21.67194393116492 28-29 21.620779859026012 27.413245895704907 29.364611817483024 21.601362427786057 30-31 21.988365006466747 26.67190383617416 29.062825413808945 22.27690574355015 32-33 21.915678516072937 26.843338560956415 29.24146291728862 21.999520005682033 34-35 22.64478817556789 26.526512923235018 29.06632397931274 21.76237492188435 36-37 22.36517911650911 26.300814574089635 29.59154725255778 21.74245905684347 38-39 22.04929239902345 26.855009862461166 29.549231174858075 21.546466563657308 40-41 22.116492764381878 26.968758878410455 29.10573499491353 21.809013362294134 42-43 21.723946540439204 27.190159710810192 28.579187819294177 22.506705929456427 44-45 22.188025175187274 26.66711439611512 28.554308341056426 22.59055208764118 46-47 22.59538657321456 27.25982003892374 28.19747231971136 21.947321068150337 48-49 22.536139625950163 27.084152836132425 28.24481939387413 22.134888144043284 50-51 21.735283411552402 27.51825835576994 28.75350582483148 21.992952407846175 52-53 22.365887983760384 27.859617699990952 27.64679921688755 22.127695099361112 54-55 21.769460676551418 27.13986965691289 28.157423243868045 22.933246422667647 56-57 22.405023558671 27.29217334324634 27.821599056965816 22.48120404111684 58-59 23.025481291485892 27.023950231678047 27.678660159423824 22.27190831741224 60-61 22.289454585862963 27.123010451875945 28.689302564825176 21.89823239743592 62-63 22.078750571353616 27.15367378944631 28.433792287327574 22.333783351872494 64-65 21.8271329151989 27.782856488243553 28.100380903284933 22.289629693272616 66-67 22.030745270371476 27.22589748884413 27.7093209846666 23.034036256117794 68-69 21.66483468016322 27.367945181312248 28.077694366226225 22.889525772298303 70-71 22.35375924905519 27.28436913968751 27.640784968273408 22.72108664298389 72-73 22.19757494040166 26.817104981287095 28.17171424414642 22.813605834164825 74-75 21.660932128345213 28.072307306338327 27.50083913087807 22.76592143443839 76-77 22.01344581671365 27.07548468312434 27.622593789518056 23.28847571064396 78-79 21.975933827189344 27.239793384666406 27.845299690180102 22.938973097964148 80-81 22.163121887626342 27.30307058894959 27.68725219863746 22.846555324786607 82-83 21.942885831232164 27.189916797166312 27.994007517237986 22.873189854363538 84-85 21.747250915597753 27.32920393114201 27.745304017403516 23.17824113585672 86-87 21.66237155907386 27.408924358781306 27.73604090032339 23.19266318182144 88-89 21.72132833388707 27.251426409905694 27.338974250550503 23.688271005656738 90-91 21.7665488685809 26.82460115989639 27.626620489970303 23.782229481552406 92-93 22.009601031930508 27.325165792786798 27.533373351952683 23.131859823330014 94-95 21.768100310793457 27.122299459175853 27.56115345269421 23.54844677733648 96-97 21.05884783922062 27.05727295782001 28.07201037090476 23.811868832054607 98-99 21.452781847737555 27.35018220309373 27.151311048919325 24.04572490024939 100-101 21.406096712448072 26.582784925916492 27.876137178392828 24.13498118324261 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1845.0 1 1309.0 2 942.5 3 1132.0 4 984.0 5 968.0 6 1645.0 7 3134.0 8 4824.0 9 5586.0 10 4949.5 11 4188.0 12 4243.5 13 4479.0 14 4783.5 15 5115.5 16 5305.0 17 5602.5 18 6009.0 19 6380.5 20 7152.0 21 8323.5 22 9898.5 23 11985.5 24 14163.5 25 17249.0 26 21340.0 27 25858.5 28 29815.5 29 34308.0 30 39621.5 31 45023.5 32 50026.0 33 54996.0 34 61472.5 35 67871.0 36 75421.0 37 82751.5 38 87518.0 39 90684.5 40 93805.0 41 98335.0 42 102757.5 43 107912.0 44 114373.0 45 118988.0 46 123589.5 47 145715.0 48 191430.5 49 189006.0 50 146863.5 51 129067.0 52 121053.5 53 116922.0 54 112990.0 55 105483.0 56 96505.5 57 87178.0 58 78076.5 59 68213.5 60 56928.5 61 45693.5 62 35929.5 63 27723.0 64 20503.0 65 14652.0 66 10484.0 67 8032.0 68 5648.0 69 3508.0 70 2303.0 71 1477.5 72 932.5 73 633.0 74 423.0 75 259.5 76 165.5 77 73.0 78 33.0 79 20.5 80 9.0 81 4.5 82 4.0 83 5.5 84 4.5 85 4.0 86 3.0 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40581858505923174 2 0.0 3 0.0 4 0.0 5 3.4367134937973053E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011026122459266352 16-17 0.0023913798061006245 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0025775351203479787 36-37 0.007202778697416851 38-39 0.01258696317103263 40-41 0.0026348136785779336 42-43 6.30064140529506E-4 44-45 4.5822846583964066E-4 46-47 2.4343387247730908E-4 48-49 2.1479459336233158E-4 50-51 5.441463031845733E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.5775351203479786E-4 60-61 0.0024629780038880685 62-63 0.0 64-65 2.2911423291982033E-4 66-67 0.0014033246766338996 68-69 0.0014892425139788321 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12538276396537168 78-79 5.727855822995508E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1484373836529286 88-89 0.43883967387880085 90-91 0.3362824153680663 92-93 0.0 94-95 0.0 96-97 4.725481053971294E-4 98-99 0.0 100-101 1.8615531424735402E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3491708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.31390518298054 #Duplication Level Percentage of deduplicated Percentage of total 1 69.07498213453499 31.99132173094929 2 15.409171206544645 14.27317788416445 3 6.145838639119746 8.539133640062703 4 3.027617293640298 5.608831210720357 5 1.7077286762964026 3.9545791996124233 6 1.097143461532045 3.0487798949713314 7 0.7350698854263671 2.3830769883550795 8 0.5171925582238229 1.9162565682337023 9 0.3858985651326753 1.6085222600222666 >10 1.7608999771978235 14.096080281321175 >50 0.09243380859487976 2.927733854090104 >100 0.04120123790080247 3.384779568586442 >500 0.0021021397349143835 0.687750487234465 >1k 0.0018548291730387317 1.8723432851022233 >5k 6.182763910129106E-4 1.8770107565487133 >10k+ 2.4731055640516423E-4 1.830622390025313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 25820 0.7394661867487201 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14286 0.4091407414365691 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12375 0.3544110790478471 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11449 0.3278911065873779 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9296 0.2662307386528312 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8285 0.23727642746758892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7957 0.2278827439178763 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6752 0.19337241258432836 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5902 0.16902902533659747 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5898 0.16891446822013753 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5664 0.1622128769072328 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5457 0.15628454613043244 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5207 0.14912472635168805 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5132 0.14697678041806475 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4653 0.13325856572199052 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4618 0.13225619095296628 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3996 0.11444255934345027 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.863927911497754E-5 0.0 10-11 0.0 0.0 0.0 2.863927911497754E-5 0.0 12-13 0.0 0.0 0.0 2.863927911497754E-5 0.0 14-15 0.0 0.0 0.0 4.295891867246631E-5 0.0 16-17 0.0 0.0 0.0 1.002374769024214E-4 0.0 18-19 1.431963955748877E-5 0.0 0.0 1.2887675601739893E-4 0.0 20-21 2.863927911497754E-5 0.0 0.0 3.436713493797305E-4 0.0 22-23 2.863927911497754E-5 0.0 0.0 6.014248614145284E-4 0.0 24-25 2.863927911497754E-5 0.0 0.0 0.0010882926063691466 0.0 26-27 2.863927911497754E-5 0.0 0.0 0.0017183567468986526 0.0 28-29 5.727855822995508E-5 0.0 0.0 0.004439088262821519 0.0 30-31 5.727855822995508E-5 0.0 0.0 0.013761173614746708 0.0 32-33 5.727855822995508E-5 0.0 0.0 0.030916101804618255 0.0 34-35 5.727855822995508E-5 0.0 0.0 0.05611866742579849 0.0 36-37 5.727855822995508E-5 0.0 0.0 0.09778881853809082 0.0 38-39 8.591783734493262E-5 0.0 0.0 0.15906255620458526 0.0 40-41 8.591783734493262E-5 0.0 0.0 0.22829801346504347 0.0 42-43 8.591783734493262E-5 0.0 0.0 0.300383078997442 0.0 44-45 8.591783734493262E-5 0.0 0.0 0.3902244975811265 0.0 46-47 1.1455711645991016E-4 0.0 0.0 0.4982804976819368 0.0 48-49 1.431963955748877E-4 0.0 0.0 0.6142982173767109 0.0 50-51 1.431963955748877E-4 0.0 0.0 0.7413706988098661 0.0 52-53 1.431963955748877E-4 0.0 0.0 0.8677844768233769 0.0 54-55 1.431963955748877E-4 0.0 0.0 1.0091622781744636 0.0 56-57 1.431963955748877E-4 0.0 0.0 1.1880432155266134 0.0 58-59 1.431963955748877E-4 0.0 0.0 1.3812437924362517 0.0 60-61 1.431963955748877E-4 0.0 0.0 1.5966254910204403 0.0 62-63 1.431963955748877E-4 0.0 0.0 1.8159880494016107 0.0 64-65 1.431963955748877E-4 0.0 0.0 2.0371119234483523 0.0 66-67 1.431963955748877E-4 0.0 0.0 2.296469235113589 0.0 68-69 1.431963955748877E-4 0.0 0.0 2.602207859305532 0.0 70-71 1.431963955748877E-4 0.0 0.0 2.935296995052278 0.0 72-73 1.431963955748877E-4 0.0 0.0 3.2730829725738806 0.0 74-75 1.431963955748877E-4 0.0 0.0 3.6270931017141184 0.0 76-77 1.5751603513237646E-4 0.0 0.0 4.003570745320055 0.0 78-79 1.7183567468986524E-4 0.0 0.0 4.449484321140256 0.0 80-81 2.2911423291982033E-4 2.863927911497754E-5 0.0 4.940733875799465 0.0 82-83 2.5775351203479786E-4 2.863927911497754E-5 0.0 5.421172102592772 0.0 84-85 2.7207315159228664E-4 4.295891867246631E-5 0.0 5.919180527123116 0.0 86-87 2.863927911497754E-4 5.727855822995508E-5 0.0 6.444296029335787 0.0 88-89 2.863927911497754E-4 5.727855822995508E-5 0.0 7.020618562606037 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 840 0.0 27.115604 3 GTACATG 14205 0.0 24.522202 1 TACATGG 14365 0.0 23.65183 2 ACATGGG 14430 0.0 22.22991 3 GAGTACT 12435 0.0 21.257103 12-13 CTTTGCG 1115 0.0 20.4279 2 TTGCGTT 1150 0.0 20.21881 4 GTACTTT 13250 0.0 19.806326 14-15 AGTACTT 12865 0.0 19.310858 12-13 CATGGGA 9605 0.0 18.921638 4 AGTACAT 10795 0.0 17.714943 2 GGACCGT 355 1.6079866E-9 17.376902 6 ACTTTTT 16100 0.0 16.461647 16-17 CATGGGG 6025 0.0 16.303118 4 TACTTTT 15140 0.0 16.236729 14-15 AGAGTAC 24920 0.0 15.501404 10-11 ATGGGAG 4155 0.0 15.189307 5 CTATACT 1205 0.0 14.964213 4 TACCTGG 2405 0.0 14.798017 2 CTAGACT 1315 0.0 14.795017 4 >>END_MODULE