FastQCFastQC Report
Fri 27 May 2016
SRR939039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252748
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104900.3224965475345769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80410.24720636212826816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51350.15786651778742158No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41190.12663138982792396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA176900.025.7570381
GTACATG113800.025.1911031
TACATGG118200.023.6004542
GGTATCA132050.023.3988821
ACATGGG116050.023.2622783
ATCAACG208000.021.517463
TCAACGC211000.021.2788644
CAACGCA210350.021.2770715
TATCAAC211550.021.2011532
AACGCAG212300.021.071266
GAGTACT94300.020.5073312-13
CATGGGG60000.020.3653094
ACGCAGA230300.019.0752147
AGTACTT101750.018.72665612-13
CGCAGAG230250.018.6270458
GTACTTT103800.018.54009814-15
AGAGTAC177450.017.84714910-11
GCAGAGT240900.017.1943869
CATGGGA67200.016.2804074
AGTACAT89450.015.8841312
Produced by FastQC (version 0.11.5)