##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939039_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3252748 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.810004648377312 33.0 31.0 34.0 30.0 34.0 2 31.89859620234952 33.0 31.0 34.0 30.0 34.0 3 31.943732499412803 34.0 31.0 34.0 30.0 34.0 4 35.48515901016617 37.0 35.0 37.0 33.0 37.0 5 35.520098390653075 37.0 35.0 37.0 33.0 37.0 6 35.59987309192105 37.0 35.0 37.0 33.0 37.0 7 35.51821413770756 37.0 35.0 37.0 33.0 37.0 8 35.5033058201865 37.0 35.0 37.0 33.0 37.0 9 37.11607785171184 39.0 37.0 39.0 33.0 39.0 10-11 37.002736762884794 39.0 37.0 39.0 33.0 39.0 12-13 36.8973970624223 39.0 37.0 39.0 33.0 39.0 14-15 38.11916293546257 40.0 38.0 41.0 33.0 41.0 16-17 38.03323144000089 40.0 38.0 41.0 33.0 41.0 18-19 37.9434596531917 40.0 38.0 41.0 33.0 41.0 20-21 38.052493614629846 40.0 38.0 41.0 33.0 41.0 22-23 37.99106663043064 40.0 38.0 41.0 33.0 41.0 24-25 37.914581609150176 40.0 38.0 41.0 32.5 41.0 26-27 37.80241245248633 40.0 38.0 41.0 32.0 41.0 28-29 37.67584208798222 40.0 37.5 41.0 32.0 41.0 30-31 37.484889391984865 40.0 37.0 41.0 32.0 41.0 32-33 37.301099870017595 40.0 37.0 41.0 31.0 41.0 34-35 36.99149027222521 40.0 36.0 41.0 30.5 41.0 36-37 36.73058134229888 40.0 36.0 41.0 30.0 41.0 38-39 36.50682561329682 39.0 35.5 41.0 30.0 41.0 40-41 36.36396410050824 39.0 35.5 41.0 30.0 41.0 42-43 36.16115696635583 39.0 35.0 41.0 29.5 41.0 44-45 36.110599868173004 39.0 35.0 41.0 29.0 41.0 46-47 35.912446183965066 39.0 35.0 41.0 28.0 41.0 48-49 35.69191403699272 39.0 35.0 41.0 27.0 41.0 50-51 34.59512710483567 38.0 34.0 40.0 25.5 40.5 52-53 34.69841469428311 38.0 34.5 39.5 25.5 40.5 54-55 35.19717251382524 39.0 35.0 40.5 26.0 41.0 56-57 35.11671039379626 39.0 35.0 41.0 26.0 41.0 58-59 34.9135100536531 38.5 34.5 40.0 26.0 41.0 60-61 34.609683873451004 38.0 34.0 40.0 25.0 41.0 62-63 34.235434930710895 38.0 34.0 40.0 23.5 41.0 64-65 33.85206631439017 37.0 33.5 40.0 23.0 41.0 66-67 33.45033998944892 36.5 33.0 39.5 22.0 41.0 68-69 33.0181727880549 36.0 33.0 39.0 21.5 41.0 70-71 32.576448897977954 35.5 32.0 39.0 20.0 40.0 72-73 32.135778271172555 35.0 32.0 38.0 18.5 40.0 74-75 31.663192783455713 35.0 31.0 37.0 17.5 39.5 76-77 31.23123202289264 35.0 31.0 37.0 13.5 39.0 78-79 30.83425460564421 35.0 31.0 36.0 9.5 39.0 80-81 30.37916909025845 34.5 30.5 36.0 7.0 37.5 82-83 29.911255037279247 34.0 30.0 35.0 2.0 37.0 84-85 29.499112750203828 34.0 29.5 35.0 2.0 37.0 86-87 29.05198051001799 34.0 29.0 35.0 2.0 36.0 88-89 28.57693587083906 34.0 29.0 35.0 2.0 36.0 90-91 28.12302582308866 34.0 28.5 35.0 2.0 35.5 92-93 27.624070324537897 34.0 27.0 35.0 2.0 35.0 94-95 27.091127409808568 33.0 26.0 35.0 2.0 35.0 96-97 26.440773616646602 33.0 25.0 35.0 2.0 35.0 98-99 25.587107731677953 33.0 22.0 35.0 2.0 35.0 100-101 22.40037300768458 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23626409250288916 1101 2 0.2836815290756043 1101 3 0.35380328769027614 1101 4 0.3117756424288842 1101 5 0.20998938638819453 1101 6 0.33401793421372616 1101 7 0.3147247571485181 1101 8 0.340298484823947 1101 9 0.3314759141148258 1101 10-11 0.29450718391108666 1101 12-13 0.30063166353329507 1101 14-15 0.4286944477715693 1101 16-17 0.4124595998142553 1101 18-19 0.496998081021232 1101 20-21 0.4759978480175704 1101 22-23 0.5558872892501796 1101 24-25 0.5350771998878443 1101 26-27 0.5378822990498691 1101 28-29 0.6068227545395857 1101 30-31 0.5202274564076461 1101 32-33 0.46917730465100504 1101 34-35 0.39965992439093867 1101 36-37 0.3484593102751319 1101 38-39 0.5610336034430361 1101 40-41 0.5007476110878955 1101 42-43 0.4704799922486771 1101 44-45 0.5103543878437264 1101 46-47 0.46798981985738664 1101 48-49 0.4182424198788368 1101 50-51 0.42033241853857817 1101 52-53 0.5215085467216696 1101 54-55 0.4105322521858952 1101 56-57 0.47406090394402867 1101 58-59 0.4253976796166654 1101 60-61 0.5073370731727991 1101 62-63 0.5104879304689334 1101 64-65 0.42312789080863666 1101 66-67 0.523454539893109 1101 68-69 0.3917712820617254 1101 70-71 0.3654062412661716 1101 72-73 0.36010819247707815 1101 74-75 0.1997487243592353 1101 76-77 0.08205160954657842 1101 78-79 0.061881147611547505 1101 80-81 0.07707501339256595 1101 82-83 0.20086649172985815 1101 84-85 -1.3937126043117587E-4 1101 86-87 -0.11373106503936015 1101 88-89 -0.13859322628800186 1101 90-91 -0.04354241937988945 1101 92-93 -0.16500386183586713 1101 94-95 -0.3676127495535617 1101 96-97 -0.5454044501976121 1101 98-99 -0.7453328325724335 1101 100-101 -0.3789447454436292 1102 1 0.17286588478760834 1102 2 0.2478937979112068 1102 3 0.30973969782641575 1102 4 0.13356630151280768 1102 5 0.23168117488864937 1102 6 0.25502362482431096 1102 7 0.30005562271472996 1102 8 0.24640385439383294 1102 9 0.3151465000718261 1102 10-11 0.381900829188659 1102 12-13 0.34270351748553196 1102 14-15 0.42245618476693636 1102 16-17 0.5124602438499579 1102 18-19 0.4915785206355565 1102 20-21 0.43196257351623046 1102 22-23 0.417632479413939 1102 24-25 0.4797444288643007 1102 26-27 0.5066431255119994 1102 28-29 0.357396390471969 1102 30-31 0.37265855791300595 1102 32-33 0.3690110990250588 1102 34-35 0.3286674254080637 1102 36-37 0.2774761387058007 1102 38-39 0.31405701791598517 1102 40-41 0.3291058797067379 1102 42-43 0.2288918757288556 1102 44-45 0.3818753736358502 1102 46-47 0.3477151541785375 1102 48-49 0.380134272516365 1102 50-51 0.2165453109784039 1102 52-53 0.3475683851886373 1102 54-55 0.35514174003093757 1102 56-57 0.30307431232306214 1102 58-59 0.31695839130720316 1102 60-61 0.3679463176167701 1102 62-63 0.32016416706188977 1102 64-65 0.2521472953820947 1102 66-67 0.22190114800138616 1102 68-69 0.3333765654640857 1102 70-71 0.36531374441528897 1102 72-73 0.21820047879491966 1102 74-75 0.18515520859945767 1102 76-77 0.3047389439849866 1102 78-79 0.23664300986075304 1102 80-81 0.15718694679661027 1102 82-83 0.1561210013122185 1102 84-85 0.024924943476705153 1102 86-87 0.040751778738012945 1102 88-89 -0.0235668963320812 1102 90-91 0.09884376840848219 1102 92-93 0.12497187800569165 1102 94-95 0.019783716156197784 1102 96-97 -0.19748448562401322 1102 98-99 -0.3729274081040508 1102 100-101 -0.1598583478328699 1103 1 0.19856699912183373 1103 2 0.26887520935459364 1103 3 0.2672245599815426 1103 4 0.30508118757025215 1103 5 0.17831120123889832 1103 6 0.24966833352898732 1103 7 0.11178135451565652 1103 8 0.09377171407829366 1103 9 0.30377584974336713 1103 10-11 0.3417114465749336 1103 12-13 0.32872122828320016 1103 14-15 0.3212745760900617 1103 16-17 0.31860282984672494 1103 18-19 0.41830706755982305 1103 20-21 0.5161079868760012 1103 22-23 0.4664768363156213 1103 24-25 0.45015241192459854 1103 26-27 0.5529720123492936 1103 28-29 0.5295111294785713 1103 30-31 0.4730209831854708 1103 32-33 0.4745370243875726 1103 34-35 0.42643067003879054 1103 36-37 0.42432744074405093 1103 38-39 0.5763116563125195 1103 40-41 0.4760822011973218 1103 42-43 0.3594542578127786 1103 44-45 0.5864351740243734 1103 46-47 0.5558325448214916 1103 48-49 0.48058237545545524 1103 50-51 0.3238380103669414 1103 52-53 0.3978795350004134 1103 54-55 0.4664350618939537 1103 56-57 0.3590154902336167 1103 58-59 0.3556616379662074 1103 60-61 0.497532804849925 1103 62-63 0.3717417945249011 1103 64-65 0.1728763292702311 1103 66-67 0.23749273480423483 1103 68-69 0.30182587401034766 1103 70-71 0.2260942023886443 1103 72-73 0.010665783711239385 1103 74-75 0.021211212068532603 1103 76-77 0.10205760458077862 1103 78-79 0.057998080842075694 1103 80-81 0.08538184950000272 1103 82-83 0.2410650529216518 1103 84-85 0.1676000056804412 1103 86-87 0.26622589096876226 1103 88-89 0.32724347888364136 1103 90-91 0.309181784288576 1103 92-93 0.29376966734703913 1103 94-95 0.2263244847373258 1103 96-97 0.21823513683472484 1103 98-99 0.2362085632271551 1103 100-101 0.31285965374429736 1104 1 0.19205815550469296 1104 2 0.23401603682693306 1104 3 0.19436553738951545 1104 4 0.1622463873379374 1104 5 0.13109375665907663 1104 6 0.17877937034248959 1104 7 0.21082137871216844 1104 8 0.11776830864373267 1104 9 0.26016526187505207 1104 10-11 0.28199915064680425 1104 12-13 0.2789415216400215 1104 14-15 0.35974189787777533 1104 16-17 0.36598432037924056 1104 18-19 0.4597599610589924 1104 20-21 0.3143380355275269 1104 22-23 0.40877337624934285 1104 24-25 0.32753737941269634 1104 26-27 0.3690496790061957 1104 28-29 0.42496387907400646 1104 30-31 0.49613412082120334 1104 32-33 0.411443744605954 1104 34-35 0.4344899256342316 1104 36-37 0.3273300740962881 1104 38-39 0.48933617251159944 1104 40-41 0.3748000361268069 1104 42-43 0.43683407664916984 1104 44-45 0.5084479650847626 1104 46-47 0.489418169268923 1104 48-49 0.6114858755045773 1104 50-51 0.46905777808735394 1104 52-53 0.5046298266855302 1104 54-55 0.6437117568990303 1104 56-57 0.4444385155210284 1104 58-59 0.5300386080256132 1104 60-61 0.578799963431841 1104 62-63 0.5427047130305809 1104 64-65 0.5188408419191077 1104 66-67 0.46385417618542846 1104 68-69 0.639616309668142 1104 70-71 0.41255176198805543 1104 72-73 0.41870461703968687 1104 74-75 0.3894231779659272 1104 76-77 0.36018094904152065 1104 78-79 0.2932432083649168 1104 80-81 0.1779862744968561 1104 82-83 0.36707593359529156 1104 84-85 0.497865008291388 1104 86-87 0.38599882465757673 1104 88-89 0.2906980690682026 1104 90-91 0.39626314935771134 1104 92-93 0.25079942956981327 1104 94-95 0.1952803769491176 1104 96-97 0.2506895227966126 1104 98-99 0.31034710116517417 1104 100-101 0.6383912358754174 1105 1 0.1352957817206537 1105 2 0.178625358136987 1105 3 0.26836106825659556 1105 4 0.2284083746714245 1105 5 0.2067024077694981 1105 6 0.1931903653632574 1105 7 0.22172681243861803 1105 8 0.2791605951315006 1105 9 0.2593681756679658 1105 10-11 0.2471524960670095 1105 12-13 0.2915600307311408 1105 14-15 0.3761456084162589 1105 16-17 0.35582530338410123 1105 18-19 0.3136833752108501 1105 20-21 0.39681740368342844 1105 22-23 0.2586132138685713 1105 24-25 0.3069201187493249 1105 26-27 0.22112578626197887 1105 28-29 0.37561659985453133 1105 30-31 0.36651085050253585 1105 32-33 0.4573825435435168 1105 34-35 0.47724974720954805 1105 36-37 0.4700552360470027 1105 38-39 0.5058479282557755 1105 40-41 0.564755217059421 1105 42-43 0.46897981398372224 1105 44-45 0.6096241569032159 1105 46-47 0.6606131896942529 1105 48-49 0.630155980599028 1105 50-51 0.626411515748913 1105 52-53 0.4917332741685314 1105 54-55 0.6292399797970347 1105 56-57 0.573415217781232 1105 58-59 0.3971970808561309 1105 60-61 0.623774019808657 1105 62-63 0.6147294567665185 1105 64-65 0.6276716373787892 1105 66-67 0.6892992798534721 1105 68-69 0.5379983566638629 1105 70-71 0.4872479367406868 1105 72-73 0.434785043548942 1105 74-75 0.4749459081224394 1105 76-77 0.34786869222307004 1105 78-79 0.3797662348739941 1105 80-81 0.4429325989160553 1105 82-83 0.564472608315139 1105 84-85 0.651637073996497 1105 86-87 0.5265236965574758 1105 88-89 0.4364082592533123 1105 90-91 0.45724950521752916 1105 92-93 0.6070389497171043 1105 94-95 0.5207285037539506 1105 96-97 0.2445227495395379 1105 98-99 0.2778691381291942 1105 100-101 0.46006736084422784 1106 1 0.2052225884475014 1106 2 0.17821524475754913 1106 3 0.1693545823588849 1106 4 0.1671265434367939 1106 5 0.15550301179967363 1106 6 0.15371963442073877 1106 7 0.2566479254384717 1106 8 0.21749670656351583 1106 9 0.25670243870165166 1106 10-11 0.27877475441368205 1106 12-13 0.2566277778306443 1106 14-15 0.2419151692566217 1106 16-17 0.2906440943335511 1106 18-19 0.4151412581346179 1106 20-21 0.3260245728283735 1106 22-23 0.34359642149981795 1106 24-25 0.31340931717158327 1106 26-27 0.37831184735814105 1106 28-29 0.2954833748945518 1106 30-31 0.3244542185483468 1106 32-33 0.3866095169562911 1106 34-35 0.3398875519064646 1106 36-37 0.33837005789110464 1106 38-39 0.5051320864918694 1106 40-41 0.41924542219862815 1106 42-43 0.3444852354020398 1106 44-45 0.4102432465670418 1106 46-47 0.35182215772686476 1106 48-49 0.34906121717751404 1106 50-51 0.10052424329170151 1106 52-53 0.18134242869133743 1106 54-55 0.21216416245840009 1106 56-57 0.17669860035017848 1106 58-59 0.20363223013080045 1106 60-61 0.5214202000605042 1106 62-63 0.47921565979326175 1106 64-65 0.18985889498279818 1106 66-67 0.287336594394759 1106 68-69 0.27675448360922417 1106 70-71 0.38409451967930863 1106 72-73 0.21510785311439662 1106 74-75 0.33671253132974144 1106 76-77 0.20823725789897196 1106 78-79 0.38782966629346305 1106 80-81 0.26879965451653476 1106 82-83 0.33532877156832086 1106 84-85 0.4902027911585485 1106 86-87 0.5578748839883687 1106 88-89 0.6074073845648158 1106 90-91 0.7160628169862129 1106 92-93 0.5668542336607914 1106 94-95 0.5898761363187681 1106 96-97 0.6697756665431491 1106 98-99 0.9240625928186823 1106 100-101 0.9756502252014982 1107 1 0.10197530247071285 1107 2 0.14410393734400984 1107 3 0.1407873977423506 1107 4 0.22201591674237164 1107 5 0.1664754761209366 1107 6 0.1477983617514198 1107 7 0.005866967473863838 1107 8 0.10551506630586971 1107 9 0.16965410370914924 1107 10-11 0.138750880059483 1107 12-13 0.2163887195052112 1107 14-15 0.26462846452739797 1107 16-17 0.2009486276081276 1107 18-19 0.3457427797189183 1107 20-21 0.2452124906541684 1107 22-23 0.22893897470805058 1107 24-25 0.21802374684306614 1107 26-27 0.19936633243150936 1107 28-29 0.1950340707626239 1107 30-31 0.29850952402597386 1107 32-33 0.4760088905373081 1107 34-35 0.3391633041404418 1107 36-37 0.24853116797479657 1107 38-39 0.43949679454993884 1107 40-41 0.21319951497316225 1107 42-43 0.29612761784027697 1107 44-45 0.11034550199017445 1107 46-47 0.40886071475971875 1107 48-49 0.4672978005183879 1107 50-51 0.4073282374996836 1107 52-53 0.4440233307818886 1107 54-55 0.42316938392820447 1107 56-57 0.3357493119574144 1107 58-59 0.3865840525300044 1107 60-61 0.419099557744957 1107 62-63 0.3795426902446266 1107 64-65 0.38833086745498946 1107 66-67 0.2906423120419106 1107 68-69 0.49036227528556253 1107 70-71 0.4331982722910368 1107 72-73 0.432790516024653 1107 74-75 0.4637408185538625 1107 76-77 0.7067786794608502 1107 78-79 0.7102290142983279 1107 80-81 0.6198096665963497 1107 82-83 0.7766974596691121 1107 84-85 0.7100589843253857 1107 86-87 0.7165447568552175 1107 88-89 0.8253330209191496 1107 90-91 0.8536225766490837 1107 92-93 0.785285048202212 1107 94-95 0.720671643179081 1107 96-97 0.7985059483136503 1107 98-99 0.7990751841550541 1107 100-101 0.823520469512772 1108 1 0.19718675643273897 1108 2 0.14301202086514664 1108 3 0.26772615408884093 1108 4 0.17536757719206264 1108 5 0.128905899457898 1108 6 0.16967180346082245 1108 7 0.2519769075059841 1108 8 0.22505538272385195 1108 9 0.264372368817682 1108 10-11 0.25134076509406356 1108 12-13 0.22925271190236884 1108 14-15 0.32644468863953335 1108 16-17 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-1.813422502311564 2316 80-81 -1.82984054981425 2316 82-83 -1.730546220399809 2316 84-85 -1.742429895341484 2316 86-87 -1.7401259834536447 2316 88-89 -1.6835776440286416 2316 90-91 -1.9570705763184186 2316 92-93 -1.9380829571121971 2316 94-95 -2.0331070610082236 2316 96-97 -2.1435347491864647 2316 98-99 -2.186015798237026 2316 100-101 -2.118577269497468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9126.0 3 2624.0 4 2711.0 5 2944.0 6 3446.0 7 4282.0 8 5193.0 9 6280.0 10 8076.0 11 10641.0 12 14015.0 13 19861.0 14 17199.0 15 17352.0 16 21974.0 17 28684.0 18 24917.0 19 18787.0 20 19217.0 21 20270.0 22 22390.0 23 25554.0 24 28782.0 25 33321.0 26 38366.0 27 45292.0 28 53288.0 29 63484.0 30 76430.0 31 92994.0 32 115413.0 33 147553.0 34 198295.0 35 280713.0 36 430588.0 37 658503.0 38 595909.0 39 88261.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87431802192508 19.52709606957942 14.31169293413809 23.28689297435741 2 15.012991120990351 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22.57034370258025 26.796126510606257 29.12365032767456 21.509879459138933 22-23 22.321112917235503 26.690395510473213 29.020834429469307 21.967657142821977 24-25 22.226677256176387 26.855638744681524 29.066881000640972 21.850802998501116 26-27 22.19202006675791 26.916797540394445 29.092121975955337 21.79906041689231 28-29 21.798197024538183 27.058954931459578 29.283266715722828 21.85958132827941 30-31 22.080787025463717 26.916716983889515 29.130433779329813 21.872062211316955 32-33 21.850254986555004 27.091704249745153 29.006045441799028 22.051995321900815 34-35 22.292329836965354 27.027986467701954 28.873672746989264 21.80601094834342 36-37 22.391612920368967 26.780116310757723 28.817779194945654 22.010491573927656 38-39 22.048899223058203 27.08772314585362 28.967951633616458 21.895425997471715 40-41 22.174500234251923 26.894375390484214 29.00075880946285 21.93036556580101 42-43 22.020104004767425 27.036111273299973 28.726119234456206 22.217665487476392 44-45 22.14290006628746 27.100679851964703 28.540466323236558 22.21595375851128 46-47 22.261059604551757 27.259702578904825 28.200429521401695 22.278808295141726 48-49 22.389116748558642 27.280480292855163 28.080721187055925 22.249681771530266 50-51 22.17529084749617 27.41893471890407 28.12405373371186 22.2817206998879 52-53 22.3589674187601 27.486912288930437 27.90834848118283 22.245771811126634 54-55 22.154348276758036 27.245970379263696 28.052357958784807 22.54732338519346 56-57 22.253895184956324 27.686867948971194 27.797959274133326 22.26127759193915 58-59 22.378260352599096 27.26347339601693 27.968424514855826 22.389841736528147 60-61 22.362721876594204 27.233584084080608 28.22261788719535 22.18107615212984 62-63 22.277877544766167 27.191057610055275 28.254840071955805 22.27622477322275 64-65 22.084250162920398 27.179355814751858 28.242347685263436 22.494046337064304 66-67 22.041354357606615 27.388087525906542 27.92771926170393 22.642838854782912 68-69 21.958779662399905 27.24240611500891 28.104649198759578 22.6941650238316 70-71 22.047689189514955 27.196164689404 28.059879132827177 22.696266988253868 72-73 22.22140330188679 27.023413086903208 28.00987216190024 22.745311449309757 74-75 22.001913830648085 27.39826420226591 27.819520176419772 22.780301790666236 76-77 22.136603312140323 27.114128670067807 27.941406820835287 22.807861196956587 78-79 22.075163913512725 27.15367189126509 27.937006429277027 22.834157765945157 80-81 22.096230339693097 27.298084472035487 27.847231628923936 22.75845355934748 82-83 22.14912964968187 27.16151026517533 27.927254808977064 22.76210527616573 84-85 22.088545229945453 26.978986306351004 27.86436445999996 23.06810400370358 86-87 22.07940058059255 26.880017102965816 28.167296416857607 22.873285899584026 88-89 22.25257240170744 26.81331766287758 27.903699611698958 23.030410323716026 90-91 22.074693797147127 26.89191454516661 28.06283579956508 22.970555858121184 92-93 22.264834640007134 26.835826342611767 27.93813873882866 22.961200278552436 94-95 22.270652681650407 26.57534242341878 27.967256284503677 23.186748610427138 96-97 22.27782617307787 26.4587320280302 27.910534377847775 23.352907421044158 98-99 22.120164426943916 26.45288918363172 27.858512728502095 23.568433660922274 100-101 22.487026845994233 25.815047128480877 28.40062545494092 23.297300570583968 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2654.0 1 1542.5 2 476.0 3 545.0 4 512.0 5 558.0 6 945.5 7 1791.0 8 2864.0 9 3436.5 10 3357.5 11 3374.0 12 3754.5 13 4107.0 14 4373.5 15 4715.0 16 4964.5 17 5082.5 18 5223.5 19 5540.0 20 6005.5 21 6578.0 22 7617.5 23 9275.5 24 11251.5 25 14106.5 26 18293.5 27 22915.5 28 26816.5 29 31301.0 30 37434.5 31 44391.5 32 51231.5 33 58110.5 34 66407.5 35 74299.0 36 82476.5 37 91981.0 38 96764.0 39 97109.5 40 97656.0 41 101205.0 42 104769.5 43 108528.0 44 113850.5 45 116316.5 46 116640.0 47 120175.5 48 134618.0 49 137576.0 50 125833.0 51 118908.5 52 112695.5 53 109101.5 54 105028.5 55 96218.0 56 86920.0 57 78391.0 58 69929.5 59 61075.5 60 51624.0 61 41374.0 62 32824.0 63 26184.5 64 19953.5 65 15030.5 66 10922.5 67 8232.0 68 6056.5 69 3826.5 70 2535.5 71 1792.0 72 1206.0 73 805.5 74 621.0 75 461.5 76 310.5 77 190.5 78 114.0 79 87.0 80 53.5 81 41.5 82 42.5 83 32.0 84 22.0 85 17.0 86 14.5 87 12.0 88 9.5 89 9.5 90 8.0 91 8.5 92 7.5 93 6.0 94 6.0 95 4.5 96 5.0 97 3.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3235725608008982 2 0.08718781780820402 3 0.09662599131565065 4 0.10225200353670189 5 0.11368848739588802 6 0.056229378974331855 7 0.05819694608989076 8 0.05936518906475386 9 0.062316539738092216 10-11 0.0595342768637472 12-13 0.05779728401954286 14-15 0.05584508852207426 16-17 0.05773579754718164 18-19 0.06625167396921004 20-21 0.06734305885362162 22-23 0.0828530215067383 24-25 0.09195301941619824 26-27 0.08595808836097969 28-29 0.08397514962733049 30-31 0.07794947533593134 32-33 0.07017143658223754 34-35 0.32068269659992105 36-37 0.499454614990156 38-39 0.4726926278949368 40-41 0.45441577398556543 42-43 0.4851590101661733 44-45 0.4945818120555297 46-47 0.4884946512917693 48-49 0.5064179579850637 50-51 0.5024674521358556 52-53 0.501575898286618 54-55 0.4927833327389641 56-57 0.471032493141184 58-59 0.45478469281973277 60-61 0.41428047915178184 62-63 0.48454414544256114 64-65 0.5074171131609334 66-67 0.5176392391909856 68-69 0.5298135607185064 70-71 0.5266316357738134 72-73 0.5158715031106006 74-75 0.5173932933015407 76-77 0.4915536032917398 78-79 0.47252354009594355 80-81 0.4779497212818208 82-83 0.48675765844756497 84-85 0.48827944863850503 86-87 0.48827944863850503 88-89 0.4944742107288976 90-91 0.508354781864442 92-93 0.5144111993920217 94-95 0.521143968115575 96-97 0.512397517422192 98-99 0.5159329895829619 100-101 0.4938593460052854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3252748.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.2554156659899 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34599672435878 28.879885006941645 2 14.701867925550543 12.424670805212457 3 6.089337021095628 7.719224008700895 4 3.2170230712543235 5.437465883317236 5 1.8912822491558499 3.995845878989436 6 1.2264800714630384 3.1095255135434217 7 0.84227975337532 2.4913616760145127 8 0.6216494950493706 2.101444624949111 9 0.43603841252541536 1.6582485930839823 >10 2.373136543868149 18.02665450964306 >50 0.16319652952755773 4.796458221433805 >100 0.08600831065128409 6.617299994470723 >500 0.0046069897934625376 1.3338312408567632 >1k 8.7752186501559E-4 0.6768036338385746 >5k 1.46253644169265E-4 0.40713896176126824 >10k+ 7.31268220846325E-5 0.3241414472431469 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10490 0.3224965475345769 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8041 0.24720636212826816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5135 0.15786651778742158 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4119 0.12663138982792396 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.07432361806079E-5 0.0 8 0.0 0.0 0.0 3.07432361806079E-5 0.0 9 0.0 0.0 0.0 3.07432361806079E-5 0.0 10-11 0.0 0.0 0.0 3.07432361806079E-5 0.0 12-13 0.0 0.0 0.0 6.14864723612158E-5 0.0 14-15 6.14864723612158E-5 0.0 0.0 6.14864723612158E-5 0.0 16-17 6.14864723612158E-5 0.0 0.0 1.229729447224316E-4 0.0 18-19 6.14864723612158E-5 0.0 0.0 1.229729447224316E-4 0.0 20-21 6.14864723612158E-5 0.0 0.0 1.9983103517395135E-4 0.0 22-23 6.14864723612158E-5 0.0 0.0 3.3817559798668694E-4 0.0 24-25 9.222970854182371E-5 0.0 0.0 6.148647236121581E-4 0.0 26-27 9.222970854182371E-5 0.0 0.0 9.99155175869757E-4 0.0 28-29 9.222970854182371E-5 0.0 0.0 0.00381216128639538 0.0 30-31 9.222970854182371E-5 0.0 0.0 0.012435639035055897 0.0 32-33 9.222970854182371E-5 0.0 0.0 0.027576682854005292 0.0 34-35 9.222970854182371E-5 0.0 0.0 0.050449650572377575 0.0 36-37 9.222970854182371E-5 0.0 0.0 0.08878646608959563 0.0 38-39 9.222970854182371E-5 0.0 0.0 0.1446161829935796 0.0 40-41 1.0760132663212765E-4 0.0 0.0 0.20787039143518032 0.0 42-43 1.229729447224316E-4 0.0 0.0 0.27197003887174787 0.0 44-45 1.8445941708364742E-4 0.0 0.0 0.3538085335845261 0.0 46-47 2.459458894448632E-4 0.0 0.0 0.4499733763574676 0.0 48-49 2.459458894448632E-4 0.0 0.0 0.5543005483363606 0.0 50-51 2.459458894448632E-4 0.0 0.0 0.670540724335239 0.0 52-53 2.459458894448632E-4 0.0 0.0 0.7906545480928742 0.0 54-55 2.459458894448632E-4 0.0 0.0 0.9222817138001469 0.0 56-57 2.459458894448632E-4 0.0 0.0 1.0763668135373536 0.0 58-59 2.459458894448632E-4 0.0 0.0 1.255415421053214 0.0 60-61 2.459458894448632E-4 0.0 0.0 1.4565684153829315 0.0 62-63 2.459458894448632E-4 0.0 0.0 1.6639161718030415 0.0 64-65 2.459458894448632E-4 0.0 0.0 1.8814553110170231 0.0 66-67 2.459458894448632E-4 0.0 0.0 2.1270783964819904 0.0 68-69 2.459458894448632E-4 0.0 0.0 2.411714648660148 0.0 70-71 2.459458894448632E-4 0.0 0.0 2.7283238664661384 0.0 72-73 2.6131750753516717E-4 0.0 0.0 3.053018555387629 0.0 74-75 2.766891256254711E-4 0.0 0.0 3.3957287807109555 0.0 76-77 2.766891256254711E-4 0.0 0.0 3.7650933918028695 0.0 78-79 2.766891256254711E-4 0.0 0.0 4.188704443135466 0.0 80-81 3.0743236180607903E-4 0.0 0.0 4.643504507573288 0.0 82-83 3.0743236180607903E-4 0.0 0.0 5.116996459608922 0.0 84-85 3.22803979896383E-4 0.0 0.0 5.609195670860454 0.0 86-87 3.3817559798668694E-4 0.0 0.0 6.120409573689693 0.0 88-89 3.3817559798668694E-4 0.0 0.0 6.6755863042572 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17690 0.0 25.757038 1 GTACATG 11380 0.0 25.191103 1 TACATGG 11820 0.0 23.600454 2 GGTATCA 13205 0.0 23.398882 1 ACATGGG 11605 0.0 23.262278 3 ATCAACG 20800 0.0 21.51746 3 TCAACGC 21100 0.0 21.278864 4 CAACGCA 21035 0.0 21.277071 5 TATCAAC 21155 0.0 21.201153 2 AACGCAG 21230 0.0 21.07126 6 GAGTACT 9430 0.0 20.50733 12-13 CATGGGG 6000 0.0 20.365309 4 ACGCAGA 23030 0.0 19.075214 7 AGTACTT 10175 0.0 18.726656 12-13 CGCAGAG 23025 0.0 18.627045 8 GTACTTT 10380 0.0 18.540098 14-15 AGAGTAC 17745 0.0 17.847149 10-11 GCAGAGT 24090 0.0 17.194386 9 CATGGGA 6720 0.0 16.280407 4 AGTACAT 8945 0.0 15.884131 2 >>END_MODULE