FastQCFastQC Report
Fri 27 May 2016
SRR939039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252748
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103660.3186843862481816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85970.26429960144468617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53710.16512192152604505No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42950.13204219939571096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114850.024.3107191
GTATCAA197200.024.0407141
GGTATCA149900.023.4895111
TACATGG117600.023.1616632
ACATGGG117750.021.761963
GAGTACT103850.021.47879612-13
TATACCG2751.2769306E-920.7068355
TCAACGC230600.020.3517684
ATCAACG230100.020.3341223
CAACGCA231250.020.2945635
CATGGGG60400.020.1125974
AACGCAG234100.020.0880326
TATCAAC235600.019.9399952
GTACTTT113600.019.61443714-15
AGTACTT111700.019.35324512-13
ACGCAGA256500.018.1487547
TCTACGG2654.0533087E-717.9068552
CGCAGAG256050.017.9026588
AGAGTAC191450.017.4392310-11
GCAGAGT267250.016.7092559
Produced by FastQC (version 0.11.5)