##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939039_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3252748 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16923567395937 33.0 31.0 34.0 30.0 34.0 2 32.41407019541631 34.0 31.0 34.0 31.0 34.0 3 32.502346016352945 34.0 31.0 34.0 30.0 34.0 4 36.05734320642116 37.0 35.0 37.0 35.0 37.0 5 35.98041102477044 37.0 35.0 37.0 35.0 37.0 6 36.01812344516083 37.0 35.0 37.0 35.0 37.0 7 35.953241382363466 37.0 35.0 37.0 35.0 37.0 8 35.922477548214616 37.0 35.0 37.0 35.0 37.0 9 37.6007090005128 39.0 37.0 39.0 35.0 39.0 10-11 37.50895273780816 39.0 37.0 39.0 34.5 39.0 12-13 37.41967022960279 39.0 37.0 39.0 34.0 39.0 14-15 38.740859267302596 40.0 38.0 41.0 34.5 41.0 16-17 38.828263824925884 40.0 38.0 41.0 35.0 41.0 18-19 38.85928882286608 40.0 38.0 41.0 35.0 41.0 20-21 38.83168247278916 40.0 38.0 41.0 34.0 41.0 22-23 38.75695181428134 40.0 38.0 41.0 34.0 41.0 24-25 38.68422008098999 40.0 38.0 41.0 34.0 41.0 26-27 38.525195311779456 40.0 38.0 41.0 34.0 41.0 28-29 38.36508269315668 40.0 38.0 41.0 34.0 41.0 30-31 38.175136684428054 40.0 38.0 41.0 33.5 41.0 32-33 38.09490314036009 40.0 38.0 41.0 33.0 41.0 34-35 38.00230020893103 40.0 37.0 41.0 33.0 41.0 36-37 37.87341718448524 40.0 37.0 41.0 33.0 41.0 38-39 37.73587025493521 40.0 37.0 41.0 33.0 41.0 40-41 37.57076309016253 40.0 37.0 41.0 32.5 41.0 42-43 37.39000131581051 40.0 37.0 41.0 32.0 41.0 44-45 37.1440477405566 40.0 36.5 41.0 31.0 41.0 46-47 36.83974165843773 40.0 36.0 41.0 31.0 41.0 48-49 36.521214831274975 39.0 36.0 41.0 30.0 41.0 50-51 36.26021720711226 39.0 35.5 41.0 30.0 41.0 52-53 36.00206148770209 39.0 35.0 40.0 29.0 41.0 54-55 35.69588375736454 38.5 35.0 40.0 28.0 41.0 56-57 35.562549573468345 38.0 35.0 40.0 28.0 41.0 58-59 35.28396005469837 38.0 34.5 40.0 28.0 41.0 60-61 34.91900463853948 38.0 34.0 40.0 27.0 41.0 62-63 34.49750364922213 37.0 34.0 40.0 26.0 41.0 64-65 34.08249624625086 37.0 33.0 39.5 26.0 41.0 66-67 33.62383759824001 36.0 33.0 39.0 25.0 40.5 68-69 33.134274773207146 36.0 32.0 39.0 23.5 40.0 70-71 32.601077765630784 35.0 32.0 38.0 22.5 40.0 72-73 32.08079645272244 35.0 31.0 37.5 21.5 39.5 74-75 31.53932767001932 35.0 31.0 37.0 20.0 39.0 76-77 29.631416728255616 32.5 28.5 35.0 18.0 37.0 78-79 30.61054345433461 34.0 30.0 36.0 18.0 38.0 80-81 30.580352366675807 34.0 30.0 35.5 18.0 37.0 82-83 30.15846401258259 34.0 30.0 35.0 14.5 37.0 84-85 29.697603072847944 34.0 29.5 35.0 8.5 36.0 86-87 28.97871553529508 34.0 29.0 35.0 4.0 36.0 88-89 28.26981678260966 33.0 27.5 35.0 2.0 35.0 90-91 27.666750544462715 33.0 26.5 35.0 2.0 35.0 92-93 27.235212042248584 33.0 26.0 34.0 2.0 35.0 94-95 26.648329965924198 32.0 25.0 34.0 2.0 35.0 96-97 25.756705407243352 32.0 24.0 34.0 2.0 35.0 98-99 24.480899227360986 32.0 10.5 34.0 2.0 35.0 100-101 18.847358448917653 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16028388473244348 1101 2 0.19244963603569687 1101 3 0.1842059877220592 1101 4 0.14293894092379844 1101 5 0.10436444574820314 1101 6 0.14872221543777187 1101 7 0.23706658971030947 1101 8 0.09210812238346477 1101 9 0.20215633806921574 1101 10-11 0.24923532214708644 1101 12-13 0.31760768211970003 1101 14-15 0.2797497558172637 1101 16-17 0.2864621259679794 1101 18-19 0.2857409782862703 1101 20-21 0.2954545216296438 1101 22-23 0.26939615312482346 1101 24-25 0.2504038682527536 1101 26-27 0.277227390147857 1101 28-29 0.3129542486562187 1101 30-31 0.2335879032765078 1101 32-33 0.26338444549634943 1101 34-35 0.37119391261669676 1101 36-37 0.3898649644454224 1101 38-39 0.35105198853667474 1101 40-41 0.33907298082881 1101 42-43 0.3121667364364029 1101 44-45 0.42460765337822437 1101 46-47 0.4002414721669041 1101 48-49 0.3325908890545932 1101 50-51 0.31652745469430243 1101 52-53 0.320162280239785 1101 54-55 0.3623869521110237 1101 56-57 0.28166170500193033 1101 58-59 0.324089392525174 1101 60-61 0.34530282274885593 1101 62-63 0.3178797081085065 1101 64-65 0.3599618988176161 1101 66-67 0.30074317515347104 1101 68-69 0.47321689506526354 1101 70-71 0.5213959470629703 1101 72-73 0.46377673500978744 1101 74-75 0.43059469137214634 1101 76-77 0.17823766219030546 1101 78-79 0.5498993165079753 1101 80-81 0.7081711122142806 1101 82-83 0.6353220576281124 1101 84-85 0.6649360551231673 1101 86-87 0.8656640808946747 1101 88-89 0.9521702757860844 1101 90-91 0.8040621762523337 1101 92-93 0.7965493513744732 1101 94-95 0.8311390284976774 1101 96-97 0.8893333529443481 1101 98-99 1.0465666284084705 1101 100-101 1.4512283540847193 1102 1 0.19122424791527237 1102 2 0.1393364563115398 1102 3 0.13684004930430405 1102 4 0.13028119659441728 1102 5 0.10725705435039146 1102 6 0.14599816652303588 1102 7 0.14127893849516937 1102 8 0.12587802308648577 1102 9 0.19397242101745604 1102 10-11 0.20466094535155577 1102 12-13 0.26493089210020315 1102 14-15 0.34270024768287044 1102 16-17 0.26751293016869226 1102 18-19 0.21515577636108674 1102 20-21 0.21924822056205784 1102 22-23 0.2262802951004943 1102 24-25 0.22010409850661716 1102 26-27 0.19217470358626798 1102 28-29 0.22775454429081066 1102 30-31 0.15939476896353 1102 32-33 0.26055790607337315 1102 34-35 0.29584195821264814 1102 36-37 0.3168657390649088 1102 38-39 0.29008279476276044 1102 40-41 0.32567748250508544 1102 42-43 0.31528997644229406 1102 44-45 0.2407810997420441 1102 46-47 0.41872796163048775 1102 48-49 0.4202272691653448 1102 50-51 0.419344311373699 1102 52-53 0.39447778133509104 1102 54-55 0.3931593108095939 1102 56-57 0.45712911946066725 1102 58-59 0.4310301874340041 1102 60-61 0.4176225904696125 1102 62-63 0.590965056607331 1102 64-65 0.4866566010244 1102 66-67 0.4076312257595731 1102 68-69 0.36939190043963777 1102 70-71 0.4953611371556619 1102 72-73 0.4647657739663913 1102 74-75 0.5503038270851199 1102 76-77 0.4391349682556118 1102 78-79 0.64979180696292 1102 80-81 0.701147902944431 1102 82-83 0.5585072538680116 1102 84-85 0.8119748456152465 1102 86-87 0.9486158785981793 1102 88-89 0.6820795822351435 1102 90-91 0.8368214961151033 1102 92-93 0.7671154765181605 1102 94-95 0.8531740436080817 1102 96-97 0.6959229929417177 1102 98-99 0.9828267486521582 1102 100-101 0.9906813146057765 1103 1 0.07670621101792108 1103 2 0.11360255715590739 1103 3 0.015072632695776633 1103 4 0.060430293219596365 1103 5 0.053699332956675505 1103 6 0.02742180586552223 1103 7 0.039835269812456886 1103 8 0.07478353460838605 1103 9 0.10614560006105478 1103 10-11 0.07890296028045185 1103 12-13 0.10910649372353731 1103 14-15 0.1268343830640788 1103 16-17 0.09298610256789175 1103 18-19 0.14728398337231852 1103 20-21 0.16318355315751631 1103 22-23 0.13786837436457944 1103 24-25 0.14806076484106256 1103 26-27 0.14355448558031725 1103 28-29 0.11949458338780516 1103 30-31 0.16892553511420516 1103 32-33 0.10042208511521977 1103 34-35 0.0611211056674108 1103 36-37 0.01880373728491236 1103 38-39 -0.03735637409393178 1103 40-41 0.050886911944282076 1103 42-43 0.0929699825971042 1103 44-45 -0.005586451327964426 1103 46-47 0.12477805071777226 1103 48-49 -0.07574609906851748 1103 50-51 -0.018046296605511714 1103 52-53 0.029940729241317854 1103 54-55 0.14945049947355926 1103 56-57 0.1345729219054519 1103 58-59 -0.050717846788103316 1103 60-61 0.021206233728783275 1103 62-63 0.1384915729708922 1103 64-65 -6.86272254839082E-4 1103 66-67 0.05256909633733642 1103 68-69 0.059449007227229345 1103 70-71 0.11256556231971615 1103 72-73 0.06421787403091628 1103 74-75 0.13408631247184033 1103 76-77 -0.16166972425773452 1103 78-79 0.2062591812571739 1103 80-81 0.1748557875628407 1103 82-83 0.2234826608806415 1103 84-85 0.22723843120811082 1103 86-87 0.2621091494042993 1103 88-89 0.27123342347967494 1103 90-91 0.4046912127318514 1103 92-93 0.4866887580252488 1103 94-95 0.7131920359180768 1103 96-97 0.47865317298721166 1103 98-99 0.5634685574300988 1103 100-101 0.8163175368501463 1104 1 0.09377108316763838 1104 2 0.09452195555211063 1104 3 0.05424353174084473 1104 4 -0.003867103242960468 1104 5 -0.01565580595782734 1104 6 0.08168364000913897 1104 7 0.13476132741875801 1104 8 0.06745650776301204 1104 9 0.013866035598205428 1104 10-11 0.09856499330420831 1104 12-13 0.11215443090222976 1104 14-15 0.13295793950277357 1104 16-17 0.1689714769502686 1104 18-19 0.1797064864701312 1104 20-21 0.15128435126315054 1104 22-23 0.1465271570595874 1104 24-25 0.0779897694580498 1104 26-27 8.822233273875213E-4 1104 28-29 0.06234573435919799 1104 30-31 0.018624974492816193 1104 32-33 0.08995513471978 1104 34-35 0.11909847831260123 1104 36-37 0.22505236933770334 1104 38-39 0.19439359445405557 1104 40-41 0.24668475045843508 1104 42-43 0.14881928177728554 1104 44-45 0.26321269783041856 1104 46-47 0.25465989885537965 1104 48-49 0.20995818478203176 1104 50-51 0.25557632058124824 1104 52-53 0.2567489615691727 1104 54-55 0.3524831630369647 1104 56-57 0.23713804911808012 1104 58-59 0.29059812273532515 1104 60-61 0.3824888468827936 1104 62-63 0.34995585382273475 1104 64-65 0.34472233680781983 1104 66-67 0.3750398158043282 1104 68-69 0.4357383243996864 1104 70-71 0.38213000792841 1104 72-73 0.4604899916907499 1104 74-75 0.30660815223373206 1104 76-77 0.20459749464311727 1104 78-79 0.4012660877748928 1104 80-81 0.4072788507650209 1104 82-83 0.44879929218892656 1104 84-85 0.6666796236961616 1104 86-87 0.5645650778074476 1104 88-89 0.48479572604288634 1104 90-91 0.68895742861503 1104 92-93 0.7878962999598293 1104 94-95 1.0292326495167181 1104 96-97 0.7867708948617889 1104 98-99 0.8784659766415075 1104 100-101 0.8839461540472442 1105 1 -0.03584164875166351 1105 2 0.02960328644763166 1105 3 0.05898708884023307 1105 4 0.014910621263268808 1105 5 -0.039077238890598665 1105 6 0.01985325416321615 1105 7 0.03611829004341871 1105 8 0.08634728055341867 1105 9 0.07326684137061079 1105 10-11 0.13025438601138006 1105 12-13 0.10747655251408617 1105 14-15 0.06976150033212036 1105 16-17 -0.046681218282628834 1105 18-19 0.07594540624800317 1105 20-21 0.12194417002823599 1105 22-23 0.09338200902381999 1105 24-25 -0.044790886213483816 1105 26-27 -0.015844132549467815 1105 28-29 0.1510826763243358 1105 30-31 0.08109490279763776 1105 32-33 0.23260599563250395 1105 34-35 0.1838173436229127 1105 36-37 0.2606355409403136 1105 38-39 0.29453478771595343 1105 40-41 0.32974596599786565 1105 42-43 0.35350214195423035 1105 44-45 0.1987732597761891 1105 46-47 0.24542272568414347 1105 48-49 0.22196961820021244 1105 50-51 0.1974357583459394 1105 52-53 0.10506594725482898 1105 54-55 0.31790656824715313 1105 56-57 0.3110935553572389 1105 58-59 0.3635361944168807 1105 60-61 0.38587273756616725 1105 62-63 0.34413184466645674 1105 64-65 0.2583976238142114 1105 66-67 0.3997289436676823 1105 68-69 0.3054520929568483 1105 70-71 0.4847257966562779 1105 72-73 0.43633725771060483 1105 74-75 0.3695537987890205 1105 76-77 0.12089620440239912 1105 78-79 0.26458166408269435 1105 80-81 0.1539350911580435 1105 82-83 0.16311872022847496 1105 84-85 0.20611154648780072 1105 86-87 0.3040726641776139 1105 88-89 0.4437605827163935 1105 90-91 0.2703065268261007 1105 92-93 0.6195626146368625 1105 94-95 0.7363861788798935 1105 96-97 0.7774301473209597 1105 98-99 0.9604133153018033 1105 100-101 0.8013874817748601 1106 1 0.062448170204326914 1106 2 0.06389396358855493 1106 3 0.09370644586167032 1106 4 0.0048768714901896715 1106 5 0.049007605893450545 1106 6 0.08125267323802632 1106 7 0.10393800519203467 1106 8 0.06501849670049609 1106 9 0.06057931970969577 1106 10-11 0.13062509714963255 1106 12-13 0.055273121424683325 1106 14-15 -0.010390910012304744 1106 16-17 -0.06821217070287844 1106 18-19 -0.011127976816048601 1106 20-21 0.07636155322430938 1106 22-23 0.04078703105606962 1106 24-25 0.1760528993723085 1106 26-27 0.15715790563976384 1106 28-29 0.053326953716116066 1106 30-31 0.039693462037156735 1106 32-33 0.14992235230736384 1106 34-35 0.039068569011988075 1106 36-37 -0.021805293809578075 1106 38-39 0.09370012034513309 1106 40-41 0.2058892498755469 1106 42-43 0.10729857883637095 1106 44-45 0.16919269766505352 1106 46-47 0.23117438016569025 1106 48-49 0.2675745148054247 1106 50-51 0.24082030771631935 1106 52-53 0.33524992065109416 1106 54-55 0.43001811578564286 1106 56-57 0.4422413364540603 1106 58-59 0.3727633328652118 1106 60-61 0.3962237682132681 1106 62-63 0.39151334913066904 1106 64-65 0.3645148733314798 1106 66-67 0.3360699814113133 1106 68-69 0.2677858196249474 1106 70-71 0.3248968972784638 1106 72-73 0.35042366208074327 1106 74-75 0.38701373640557435 1106 76-77 0.2859284688553885 1106 78-79 0.43206326261718075 1106 80-81 0.45404318945322686 1106 82-83 0.47528328544227705 1106 84-85 0.5273736972312868 1106 86-87 0.7459632290943574 1106 88-89 0.6947751388532417 1106 90-91 0.8978047526937765 1106 92-93 0.8896959221146226 1106 94-95 0.9182415499623637 1106 96-97 0.7835310995761695 1106 98-99 0.9467473390339194 1106 100-101 1.3008016055185259 1107 1 -0.028601652139791156 1107 2 -0.028855098947104807 1107 3 0.016928804927239582 1107 4 0.018180306957624737 1107 5 0.04735174679050402 1107 6 0.011441090782476238 1107 7 -0.01582980269564871 1107 8 0.007116547797714645 1107 9 -0.05081849569344854 1107 10-11 -0.009010708282737312 1107 12-13 -8.658928310723013E-4 1107 14-15 -0.015676123455129698 1107 16-17 -0.0014244763733017862 1107 18-19 -0.008747771577262142 1107 20-21 0.05961355111526245 1107 22-23 -0.00636599893098122 1107 24-25 -0.038325275513479085 1107 26-27 0.0034699720748037066 1107 28-29 0.009758716542556556 1107 30-31 0.030849081117750643 1107 32-33 0.07758286233517708 1107 34-35 -0.005504712565702619 1107 36-37 -0.09340056694320253 1107 38-39 -0.04215479204670913 1107 40-41 -0.053754041499786354 1107 42-43 0.06216439257116235 1107 44-45 0.03279458715246619 1107 46-47 0.0530152335435119 1107 48-49 0.03383224175443189 1107 50-51 0.003106992771584771 1107 52-53 -0.03922247284390323 1107 54-55 -0.2340909684700705 1107 56-57 0.053468037563284554 1107 58-59 0.07743216104462647 1107 60-61 0.1606297325719268 1107 62-63 0.14023409886605265 1107 64-65 0.09993661424112332 1107 66-67 0.04707731783214797 1107 68-69 0.041410560420480635 1107 70-71 0.11305951635513622 1107 72-73 0.16440196366686166 1107 74-75 0.11821693696589008 1107 76-77 0.056539699540472554 1107 78-79 0.3171861560275815 1107 80-81 0.17082193212959496 1107 82-83 0.13857034233544496 1107 84-85 0.2441822951466328 1107 86-87 0.4351452053832965 1107 88-89 0.49832530549170784 1107 90-91 0.5276140529696036 1107 92-93 0.6773688562501299 1107 94-95 0.672367524315387 1107 96-97 0.8627786098361447 1107 98-99 1.0011598646592574 1107 100-101 1.0571690836705905 1108 1 0.0030311409321726046 1108 2 0.02314040346197288 1108 3 0.060186041062095796 1108 4 0.05158540260833888 1108 5 0.0016744044166614458 1108 6 0.03833748995743491 1108 7 0.06019707032916699 1108 8 0.08370916125745964 1108 9 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-0.9865627016695413 2316 50-51 -1.074604096318886 2316 52-53 -1.0438069771192602 2316 54-55 -0.998029175867643 2316 56-57 -1.0459735000170554 2316 58-59 -1.1226601073455456 2316 60-61 -0.9343808709951062 2316 62-63 -0.8530964626022879 2316 64-65 -0.9131974893235366 2316 66-67 -0.9031665089825651 2316 68-69 -0.8565435191914759 2316 70-71 -0.9532893457338041 2316 72-73 -0.8217145824736427 2316 74-75 -1.1557463355599857 2316 76-77 -1.083198627462803 2316 78-79 -1.0699005297107789 2316 80-81 -1.105880989689613 2316 82-83 -1.0903250484265108 2316 84-85 -1.3101423665096341 2316 86-87 -1.2768247937804098 2316 88-89 -1.6000828576769912 2316 90-91 -1.7390294052449242 2316 92-93 -1.6665504635436683 2316 94-95 -1.608614615301402 2316 96-97 -1.533838623484936 2316 98-99 -1.6237813186798888 2316 100-101 -1.0115963897266056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 27.0 5 81.0 6 167.0 7 421.0 8 937.0 9 1808.0 10 2961.0 11 4573.0 12 6812.0 13 9073.0 14 11292.0 15 14729.0 16 19539.0 17 27472.0 18 26910.0 19 19177.0 20 18743.0 21 20649.0 22 22084.0 23 24498.0 24 28788.0 25 33746.0 26 40497.0 27 48995.0 28 59452.0 29 71626.0 30 87012.0 31 105619.0 32 131055.0 33 166989.0 34 222912.0 35 319445.0 36 498200.0 37 731550.0 38 454120.0 39 20779.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.69700400344819 20.267151693679885 14.89967305121642 23.136171251655497 2 15.355816066907119 21.24861809153368 39.893806713584944 23.501759127974253 3 18.682326451357437 25.240965485183604 30.660782821171512 25.415925242287447 4 12.946253444779614 17.508980099288355 37.28244548916793 32.2623209667641 5 13.440297250201983 37.75788963670103 34.624139343103124 14.177673769993865 6 31.272942139999778 36.820067217011584 17.587006432714738 14.319984210273898 7 27.755608488576428 33.261691345287126 21.40282616421561 17.579874001920835 8 25.38997795095101 35.95599782091942 20.115268689735572 18.538755538393996 9 26.012620713316863 17.018994401041827 21.04678874600799 35.92159613963332 10-11 24.581046548948766 26.883146189006958 27.688864922828326 20.84694233921595 12-13 25.37422204240845 24.413588141472996 28.21938557797899 21.992804238139566 14-15 22.44675758757986 25.70875363580662 26.52224278964324 25.322245986970277 16-17 21.76447213791121 28.89714534740279 27.252205686247233 22.08617682843877 18-19 21.645767603441715 27.68557253697847 29.39776111659794 21.270898742981874 20-21 22.479054633190152 26.887527100162696 29.16596981997991 21.467448446667248 22-23 22.174850311183036 26.821198568103032 29.055324912965897 21.948626207748035 24-25 22.14855968640655 26.955405224477225 29.160910633521546 21.73512445559468 26-27 22.079119267634514 27.031486458755253 29.13214136664466 21.757252906965576 28-29 21.71672997723771 27.14337231165771 29.338101199355133 21.80179651174945 30-31 22.028404905636712 26.975053093568885 29.15703890986944 21.839503090924968 32-33 21.801627423950457 27.11598008822079 29.07749078625212 22.00490170157664 34-35 22.231818735964907 27.08641797279096 28.976442642732298 21.705320648511833 36-37 22.315590664303805 26.849490050864222 28.881414493459545 21.953504791372424 38-39 22.01194193220094 27.143974996963788 29.031224586113968 21.812858484721307 40-41 22.08962253926787 26.95180278571041 29.047303765168614 21.911270909853105 42-43 21.944047752640582 27.128923213618666 28.763033823762257 22.163995209978495 44-45 22.05897634391677 27.186326165326342 28.609961465788054 22.144736024968843 46-47 22.236140524605393 27.3269086422205 28.190065426952255 22.246885406221857 48-49 22.3231227933391 27.375630716104887 28.089525101363442 22.211721389192572 50-51 22.113547696857715 27.489078404909108 28.073541302229206 22.323832596003975 52-53 22.323555344588637 27.55897474996526 27.830145464696308 22.28732444074979 54-55 22.132393902017615 27.34861415639945 27.962756413961365 22.556235527621567 56-57 22.193034935383864 27.755070481943267 27.777743618626467 22.2741509640464 58-59 22.306556574444063 27.33784185733358 27.952003357182548 22.403598211039814 60-61 22.290867715517745 27.35688667452475 28.143079200000493 22.209166409957014 62-63 22.189522977543177 27.33494255633421 28.19006068327365 22.285473782848968 64-65 22.032786330856183 27.273388256470916 28.11811010913149 22.575715303541415 66-67 21.977963023910053 27.50296333519283 27.806237307540776 22.71283633335634 68-69 21.872616376181607 27.33346491782272 28.02539027252439 22.768528433471282 70-71 21.9824437675467 27.301100484882323 27.925633956273284 22.790821791297695 72-73 22.108429549414833 27.154163187557106 27.837815902123374 22.899591360904687 74-75 21.831525221136097 27.542803807734263 27.631805476477123 22.99386549465252 76-77 21.924696968834443 27.191284235486634 27.84861911825366 23.03539967742526 78-79 21.84309312771511 27.321090932568413 27.797320937210813 23.038495002505666 80-81 21.790145603354215 27.46993996755352 27.72992070380027 23.00999372529199 82-83 21.849755960191196 27.330875309123236 27.790332973842425 23.029035756843136 84-85 21.75032634005402 27.068931381585138 27.76346848289843 23.417273795462414 86-87 21.685378754181315 27.00593419169275 28.01277276718518 23.295914286940764 88-89 21.80260806653469 26.928668321140925 27.784267425785654 23.48445618653873 90-91 21.610376904273966 26.983778178316587 27.96012752771537 23.44571738969408 92-93 21.702957007428797 27.00066220930733 27.79203922345045 23.504341559813426 94-95 21.611961929087336 26.731355101477394 27.81990577422984 23.83677719520543 96-97 21.490094143489465 26.627366557041395 27.89349619052816 23.98904310894098 98-99 21.289629568598613 26.641381379682656 27.738561364114283 24.33042768760445 100-101 21.443354592366116 25.919264864798393 28.622528426078365 24.01485211675713 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 921.0 1 620.5 2 356.5 3 418.0 4 397.5 5 426.0 6 702.0 7 1379.0 8 2231.0 9 2726.0 10 2712.0 11 2697.0 12 2934.5 13 3214.0 14 3527.0 15 3813.5 16 3958.5 17 4152.5 18 4504.5 19 4882.5 20 5482.0 21 6379.0 22 7674.5 23 9419.0 24 11363.0 25 14278.0 26 18650.0 27 23323.5 28 27139.0 29 31766.0 30 38210.0 31 45357.0 32 51785.5 33 58496.0 34 66577.0 35 73985.0 36 82899.0 37 92442.5 38 97136.0 39 97576.5 40 98132.0 41 101891.0 42 105159.0 43 109386.5 44 114738.0 45 117210.0 46 117684.0 47 121280.0 48 136357.0 49 139543.0 50 127578.0 51 119929.0 52 113355.0 53 109396.0 54 104790.0 55 96305.5 56 87017.0 57 78505.0 58 69947.0 59 60645.5 60 51184.5 61 40717.0 62 32266.5 63 25823.5 64 19503.5 65 14570.0 66 10331.5 67 7798.0 68 5707.0 69 3362.5 70 2158.0 71 1484.0 72 976.0 73 662.5 74 486.0 75 364.0 76 233.5 77 103.5 78 36.0 79 25.0 80 17.5 81 7.0 82 6.5 83 4.5 84 2.0 85 3.5 86 3.0 87 1.0 88 1.5 89 2.0 90 3.5 91 2.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3932367339861557 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.53040321598845E-4 16-17 0.0023211143316358967 18-19 3.22803979896383E-4 20-21 0.0 22-23 0.0 24-25 4.6114854270911855E-5 26-27 1.537161809030395E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012589355215958936 36-37 0.023272629788720182 38-39 0.010544930009948512 40-41 0.005672127075322158 42-43 0.00470371513563301 44-45 0.0016601347537528268 46-47 0.0011221281205921886 48-49 0.0029513506733383587 50-51 5.533782512509423E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.302363417024621E-4 60-61 0.0014141888643079636 62-63 0.0012143578291340122 64-65 6.302363417024621E-4 66-67 0.0024133440401777205 68-69 0.0026131750753516717 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09095386424032849 78-79 0.0036277018693117325 80-81 2.1520265326425533E-4 82-83 0.0 84-85 1.8445941708364742E-4 86-87 0.09289068811970679 88-89 0.4383063182269269 90-91 0.3894553159359409 92-93 0.0 94-95 8.761822311473252E-4 96-97 1.537161809030395E-5 98-99 0.0 100-101 3.996620703479028E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3252748.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.732156631996254 #Duplication Level Percentage of deduplicated Percentage of total 1 66.64331149680196 27.145258023626546 2 15.365683224745089 12.517548317359086 3 6.434680004860022 7.862951815041985 4 3.4097572863700307 5.555470714620584 5 2.0207042275302185 4.115382055134893 6 1.2991744499772524 3.1750906313256624 7 0.8847066652918468 2.522520732482701 8 0.6622287045280091 2.1579202655231065 9 0.4800606541132353 1.759851518057899 >10 2.5219232086169963 18.38565148429894 >50 0.17774187211991832 4.989021204554678 >100 0.09353261199084376 6.895636503339057 >500 0.0052115804744971095 1.4261451090544046 >1k 0.001057422124832032 0.7429146622357922 >5k 1.5106030354743315E-4 0.42972635423677086 >10k+ 7.553015177371658E-5 0.3189106091078442 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10366 0.3186843862481816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8597 0.26429960144468617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5371 0.16512192152604505 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4295 0.13204219939571096 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.07432361806079E-5 0.0 0.0 0.0 0.0 7 3.07432361806079E-5 0.0 0.0 3.07432361806079E-5 0.0 8 3.07432361806079E-5 0.0 0.0 3.07432361806079E-5 0.0 9 3.07432361806079E-5 0.0 0.0 3.07432361806079E-5 0.0 10-11 3.07432361806079E-5 0.0 0.0 3.07432361806079E-5 0.0 12-13 6.14864723612158E-5 0.0 0.0 6.14864723612158E-5 0.0 14-15 6.14864723612158E-5 0.0 0.0 6.14864723612158E-5 0.0 16-17 6.14864723612158E-5 0.0 0.0 9.222970854182371E-5 0.0 18-19 6.14864723612158E-5 0.0 0.0 9.222970854182371E-5 0.0 20-21 6.14864723612158E-5 0.0 0.0 1.6908779899334347E-4 0.0 22-23 6.14864723612158E-5 0.0 0.0 2.920607437157751E-4 0.0 24-25 6.14864723612158E-5 0.0 0.0 5.533782512509422E-4 0.0 26-27 6.14864723612158E-5 0.0 0.0 9.53040321598845E-4 0.0 28-29 1.0760132663212765E-4 0.0 0.0 0.0038736477587565958 0.0 30-31 1.229729447224316E-4 0.0 0.0 0.012558611979778329 0.0 32-33 1.229729447224316E-4 0.0 0.0 0.027853371979630764 0.0 34-35 1.229729447224316E-4 0.0 0.0 0.05104914367789942 0.0 36-37 1.5371618090303951E-4 0.0 0.0 0.09006231039109086 0.0 38-39 1.5371618090303951E-4 0.0 0.0 0.14670672305386093 0.0 40-41 1.5371618090303951E-4 0.0 0.0 0.2107909988723381 0.0 42-43 1.5371618090303951E-4 0.0 0.0 0.2755977407410596 0.0 44-45 1.5371618090303951E-4 0.0 0.0 0.35857373519252034 0.0 46-47 1.5371618090303951E-4 0.0 0.0 0.4559068209403249 0.0 48-49 1.5371618090303951E-4 0.0 0.0 0.5611562900046361 0.0 50-51 1.5371618090303951E-4 0.0 0.0 0.6785185941241068 0.0 52-53 1.5371618090303951E-4 0.0 0.0 0.7992780258415346 0.0 54-55 1.5371618090303951E-4 0.0 0.0 0.9320734345236705 0.0 56-57 1.5371618090303951E-4 0.0 0.0 1.0876495812156368 0.0 58-59 1.5371618090303951E-4 0.0 0.0 1.26785106008827 0.0 60-61 1.5371618090303951E-4 0.0 0.0 1.4709716215335464 0.0 62-63 1.5371618090303951E-4 0.0 0.0 1.6803330599234862 0.0 64-65 1.5371618090303951E-4 0.0 0.0 1.9001779418510134 0.0 66-67 1.5371618090303951E-4 0.0 0.0 2.1478454525219908 0.0 68-69 1.5371618090303951E-4 0.0 0.0 2.434802819031785 0.0 70-71 1.5371618090303951E-4 0.0 0.0 2.7539022389684047 0.0 72-73 1.5371618090303951E-4 0.0 0.0 3.08036466397028 0.0 74-75 1.5371618090303951E-4 0.0 0.0 3.425918638640313 0.0 76-77 1.5371618090303951E-4 0.0 0.0 3.798603519239732 0.0 78-79 1.5371618090303951E-4 0.0 0.0 4.222952408240663 0.0 80-81 2.766891256254711E-4 0.0 0.0 4.679182033160885 0.0 82-83 2.766891256254711E-4 0.0 0.0 5.154088174060824 0.0 84-85 2.766891256254711E-4 0.0 0.0 5.647993634920381 0.0 86-87 2.766891256254711E-4 0.0 0.0 6.1605602401415664 0.0 88-89 2.766891256254711E-4 0.0 0.0 6.7179043688598075 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11485 0.0 24.310719 1 GTATCAA 19720 0.0 24.040714 1 GGTATCA 14990 0.0 23.489511 1 TACATGG 11760 0.0 23.161663 2 ACATGGG 11775 0.0 21.76196 3 GAGTACT 10385 0.0 21.478796 12-13 TATACCG 275 1.2769306E-9 20.706835 5 TCAACGC 23060 0.0 20.351768 4 ATCAACG 23010 0.0 20.334122 3 CAACGCA 23125 0.0 20.294563 5 CATGGGG 6040 0.0 20.112597 4 AACGCAG 23410 0.0 20.088032 6 TATCAAC 23560 0.0 19.939995 2 GTACTTT 11360 0.0 19.614437 14-15 AGTACTT 11170 0.0 19.353245 12-13 ACGCAGA 25650 0.0 18.148754 7 TCTACGG 265 4.0533087E-7 17.906855 2 CGCAGAG 25605 0.0 17.902658 8 AGAGTAC 19145 0.0 17.43923 10-11 GCAGAGT 26725 0.0 16.709255 9 >>END_MODULE