FastQCFastQC Report
Fri 27 May 2016
SRR939038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260464
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105990.3250764308392916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80280.2462226235284303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52150.15994655975345842No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40330.1236940509080916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA178300.026.0859641
GGTATCA131900.024.9102251
GTACATG109900.023.684481
ATCAACG206150.022.2941573
TACATGG114300.022.0717162
TCAACGC208650.022.0497194
TATCAAC210050.021.9703562
CAACGCA209800.021.9291925
GAGTACT92950.021.61607412-13
AACGCAG213400.021.4904026
ACATGGG115150.021.3333263
ACGCAGA230850.019.6817267
GTACTTT101450.019.52518714-15
CGCAGAG230450.019.2033378
CATGGGG56850.018.8162524
AGTACTT102400.018.4425712-13
GTGTAGC14400.018.108791
GCAGAGT242250.017.662549
AGAGTAC178150.017.2959210-11
CATGGGA66250.016.5036984
Produced by FastQC (version 0.11.5)