##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939038_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3260464 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811502595949534 33.0 31.0 34.0 30.0 34.0 2 31.88715471172201 33.0 31.0 34.0 30.0 34.0 3 31.934432951874335 34.0 31.0 34.0 30.0 34.0 4 35.47616075503364 37.0 35.0 37.0 33.0 37.0 5 35.50711187119379 37.0 35.0 37.0 33.0 37.0 6 35.583948787657214 37.0 35.0 37.0 33.0 37.0 7 35.49329083222511 37.0 35.0 37.0 33.0 37.0 8 35.47739984247641 37.0 35.0 37.0 33.0 37.0 9 37.09301283498299 39.0 37.0 39.0 33.0 39.0 10-11 36.98020511804455 39.0 37.0 39.0 33.0 39.0 12-13 36.85883696308255 39.0 37.0 39.0 33.0 39.0 14-15 38.083102589079346 40.0 38.0 41.0 33.0 41.0 16-17 38.008701675589734 40.0 38.0 41.0 33.0 41.0 18-19 37.95076544320072 40.0 38.0 41.0 33.0 41.0 20-21 37.83103693216671 40.0 38.0 41.0 32.0 41.0 22-23 37.906684140662186 40.0 38.0 41.0 32.5 41.0 24-25 37.84758196992821 40.0 38.0 41.0 32.0 41.0 26-27 37.77034342351273 40.0 38.0 41.0 32.0 41.0 28-29 37.64256743825419 40.0 37.5 41.0 32.0 41.0 30-31 37.468206825776946 40.0 37.0 41.0 32.0 41.0 32-33 37.29383455851682 40.0 37.0 41.0 31.0 41.0 34-35 36.984015005226254 40.0 36.0 41.0 30.5 41.0 36-37 36.728507813611806 40.0 36.0 41.0 30.0 41.0 38-39 36.532738898512605 39.0 36.0 41.0 30.0 41.0 40-41 36.38771536811939 39.0 35.5 41.0 30.0 41.0 42-43 36.188903481222304 39.0 35.0 41.0 29.5 41.0 44-45 36.12989516216096 39.0 35.0 41.0 29.0 41.0 46-47 35.916087403510666 39.0 35.0 41.0 28.0 41.0 48-49 35.753642579706444 39.0 35.0 41.0 27.0 41.0 50-51 34.599907405817085 38.0 34.0 40.0 25.5 40.5 52-53 34.749868576987815 38.5 34.5 39.5 26.0 40.5 54-55 35.2514129890715 39.0 35.0 40.5 26.0 41.0 56-57 35.16143024428425 39.0 35.0 41.0 26.0 41.0 58-59 34.967499871183975 38.5 35.0 40.5 26.0 41.0 60-61 34.7046820636572 38.0 34.0 40.0 26.0 41.0 62-63 34.35353863744547 38.0 34.0 40.0 24.5 41.0 64-65 33.95103948395075 37.0 34.0 40.0 23.0 41.0 66-67 33.57805698820781 37.0 33.0 39.5 22.5 41.0 68-69 33.1858963938875 36.0 33.0 39.0 22.0 41.0 70-71 32.773042119158504 36.0 32.5 39.0 21.0 40.0 72-73 32.33684408108785 35.0 32.0 38.0 20.0 40.0 74-75 31.8656962628632 35.0 31.5 37.0 18.5 39.5 76-77 31.44343228448466 35.0 31.0 37.0 17.5 39.0 78-79 31.01247199785061 35.0 31.0 36.0 13.5 39.0 80-81 30.57372892324528 34.5 31.0 36.0 9.0 37.5 82-83 30.189833103509194 34.0 30.0 35.5 7.0 37.0 84-85 29.837157686758694 34.0 30.0 35.0 2.0 37.0 86-87 29.44388743442651 34.0 30.0 35.0 2.0 36.0 88-89 29.027552673484507 34.0 29.0 35.0 2.0 36.0 90-91 28.602691518753158 34.0 29.0 35.0 2.0 35.5 92-93 28.183320073461935 34.0 29.0 35.0 2.0 35.0 94-95 27.708774272618868 34.0 27.5 35.0 2.0 35.0 96-97 27.121224310404898 33.5 26.5 35.0 2.0 35.0 98-99 26.41400518453815 33.0 25.0 35.0 2.0 35.0 100-101 23.79112359467855 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2527657207510181 1101 2 0.2613057781356005 1101 3 0.2691606680467551 1101 4 0.1931391237963851 1101 5 -0.005767849922641233 1101 6 0.13189356560778265 1101 7 0.10620731673186867 1101 8 0.17430419384520235 1101 9 0.12982064470004673 1101 10-11 0.28591518586382136 1101 12-13 0.27079560176406403 1101 14-15 0.08192557230345443 1101 16-17 0.2807961610898815 1101 18-19 0.3043861922531548 1101 20-21 0.4237392695440647 1101 22-23 0.4270161187427064 1101 24-25 0.4172918692341483 1101 26-27 0.4026863824441804 1101 28-29 0.3629643811176848 1101 30-31 0.2590757047131049 1101 32-33 0.32681690714289147 1101 34-35 0.27309207050301865 1101 36-37 0.2701623605320833 1101 38-39 0.33267392842255106 1101 40-41 0.3192895891736427 1101 42-43 0.23458523432490352 1101 44-45 0.31328734649446943 1101 46-47 0.18700072383524713 1101 48-49 0.29781268195266364 1101 50-51 0.17099484059236403 1101 52-53 0.13339059490500915 1101 54-55 0.10080122921610979 1101 56-57 0.1650525606685136 1101 58-59 0.1634240751340954 1101 60-61 0.06204565920801741 1101 62-63 0.12517459458116775 1101 64-65 0.008870059650362805 1101 66-67 -0.013203373308748212 1101 68-69 -0.026576421636001157 1101 70-71 -0.057158758261124376 1101 72-73 -0.05339752197248515 1101 74-75 -0.20145142116671977 1101 76-77 -0.23864016091615525 1101 78-79 -0.4137740968765691 1101 80-81 -0.43716923650049466 1101 82-83 -0.428692874644387 1101 84-85 -0.5100871992582832 1101 86-87 -0.6921295739814148 1101 88-89 -0.7212845045732763 1101 90-91 -0.7768277069915861 1101 92-93 -0.6498181535581793 1101 94-95 -0.7381841161958 1101 96-97 -0.7981296121476831 1101 98-99 -0.9566285691038132 1101 100-101 -0.5828246157111892 1102 1 0.31797406497241454 1102 2 0.2642005501251248 1102 3 0.21687798241583423 1102 4 0.34502771007788624 1102 5 0.2963411106758116 1102 6 0.36899226400886675 1102 7 0.4103593611383758 1102 8 0.307863740721281 1102 9 0.43779044590183247 1102 10-11 0.3643406660514259 1102 12-13 0.48202927209492685 1102 14-15 0.6000420122726595 1102 16-17 0.5333689691380812 1102 18-19 0.5144052390979397 1102 20-21 0.598759739095243 1102 22-23 0.600805511740667 1102 24-25 0.5062289236705695 1102 26-27 0.5838320708050233 1102 28-29 0.511041985396254 1102 30-31 0.5218386069825272 1102 32-33 0.5208526057820606 1102 34-35 0.3947994407673576 1102 36-37 0.4010987723310606 1102 38-39 0.5212365626682853 1102 40-41 0.4188582284662772 1102 42-43 0.43412093522233874 1102 44-45 0.595492934988421 1102 46-47 0.5936456982620228 1102 48-49 0.486194816373569 1102 50-51 0.5128594118480123 1102 52-53 0.47328531463184476 1102 54-55 0.5025688370627464 1102 56-57 0.5959438717069787 1102 58-59 0.5476014185509044 1102 60-61 0.3280919161997602 1102 62-63 0.3140912923796151 1102 64-65 0.4128793172427976 1102 66-67 0.5375706305870764 1102 68-69 0.3653562846741778 1102 70-71 0.2981274831679599 1102 72-73 0.19983469919802843 1102 74-75 0.23371365471418315 1102 76-77 0.25841264310587775 1102 78-79 0.20343965040303047 1102 80-81 0.14677995771860708 1102 82-83 0.08410309506390234 1102 84-85 -0.09284515915538094 1102 86-87 -0.11840531577278668 1102 88-89 0.014365194787245628 1102 90-91 0.02028774772925246 1102 92-93 0.12749942225335786 1102 94-95 0.13088866636627117 1102 96-97 -0.10725596718680919 1102 98-99 -0.04666168816014249 1102 100-101 0.20779655532134456 1103 1 0.14359261837603654 1103 2 0.265554522210536 1103 3 0.39477797771185763 1103 4 0.3281539561881388 1103 5 0.30630056330505795 1103 6 0.29405147349961425 1103 7 0.18751019283576653 1103 8 0.2573844553905218 1103 9 0.38243981845749175 1103 10-11 0.4081809770664009 1103 12-13 0.423956502845904 1103 14-15 0.4877919830469679 1103 16-17 0.4946350380468658 1103 18-19 0.559776965833457 1103 20-21 0.5138879983763971 1103 22-23 0.5672389300347831 1103 24-25 0.5798694787991323 1103 26-27 0.5055013675163949 1103 28-29 0.49690226717802943 1103 30-31 0.47151640641571646 1103 32-33 0.481620257378772 1103 34-35 0.4935298937061674 1103 36-37 0.48940944784805396 1103 38-39 0.5511323327484874 1103 40-41 0.5824797437642033 1103 42-43 0.5071243112589414 1103 44-45 0.5856923351303536 1103 46-47 0.6716877341877137 1103 48-49 0.538484005352224 1103 50-51 0.4120439961964806 1103 52-53 0.4404645105228653 1103 54-55 0.6117359974320351 1103 56-57 0.6514144557633443 1103 58-59 0.4915348017527563 1103 60-61 0.49402287755315655 1103 62-63 0.4294770163748325 1103 64-65 0.3582514833013306 1103 66-67 0.4690848344893155 1103 68-69 0.2964403806347491 1103 70-71 0.25720770579971486 1103 72-73 0.37347352918013144 1103 74-75 0.4127446924562044 1103 76-77 0.2163418394522978 1103 78-79 0.2330128931029556 1103 80-81 0.16481127416286157 1103 82-83 0.17949581822471927 1103 84-85 0.24654858152038983 1103 86-87 0.3655450360124384 1103 88-89 0.35652661684796527 1103 90-91 0.24595951279301786 1103 92-93 0.18615799123258725 1103 94-95 0.21405315500875233 1103 96-97 0.093223462799088 1103 98-99 0.17456744975631722 1103 100-101 0.4163137212250376 1104 1 0.17440105446333476 1104 2 0.08455232329378859 1104 3 0.1234819243162768 1104 4 0.23632296950778908 1104 5 0.17350263807324495 1104 6 0.1699326693360419 1104 7 0.22716933149868623 1104 8 0.22000255735442664 1104 9 0.34535649104196153 1104 10-11 0.1296650286540526 1104 12-13 0.27635181410708043 1104 14-15 0.21785521443533895 1104 16-17 0.2630549342332671 1104 18-19 0.3322700691549727 1104 20-21 0.32669064183858865 1104 22-23 0.2750652293965743 1104 24-25 0.26816415285900774 1104 26-27 0.1928721331187191 1104 28-29 0.2387483338155718 1104 30-31 0.3121629272064297 1104 32-33 0.2808708858495592 1104 34-35 0.3227593517550602 1104 36-37 0.24362786745182774 1104 38-39 0.2907406339933942 1104 40-41 0.37612123386448104 1104 42-43 0.2602987780090942 1104 44-45 0.39660841746609066 1104 46-47 0.3103084131116134 1104 48-49 0.32287988388713984 1104 50-51 0.22778613477669296 1104 52-53 0.2619649161361295 1104 54-55 0.22203951394438093 1104 56-57 0.25098488584706047 1104 58-59 0.2524474587242551 1104 60-61 0.15030867156512073 1104 62-63 0.09828669394779865 1104 64-65 0.07416409641145094 1104 66-67 0.1832367268359647 1104 68-69 0.10800362706130073 1104 70-71 0.08093010759827735 1104 72-73 0.13981883471844725 1104 74-75 0.06433925172461841 1104 76-77 -0.03139073651350799 1104 78-79 -0.025306460288533117 1104 80-81 -0.06671102662923545 1104 82-83 -0.10140425679911047 1104 84-85 -0.11147649339900667 1104 86-87 -0.08502570486043126 1104 88-89 -0.0556195785331397 1104 90-91 -0.1915902489770751 1104 92-93 -0.19847918750375015 1104 94-95 -0.20391595071219015 1104 96-97 -0.0778270609056868 1104 98-99 -0.048436032836566056 1104 100-101 0.09597120359903144 1105 1 -0.031887420852491744 1105 2 0.0912031432900946 1105 3 0.1248179234345308 1105 4 0.11721076665311614 1105 5 0.13245460207703985 1105 6 0.17619448139982552 1105 7 0.11824184257730508 1105 8 0.09929055937726616 1105 9 0.15916189630718947 1105 10-11 0.12081874513859248 1105 12-13 0.21480184441728767 1105 14-15 0.06562384872574256 1105 16-17 0.19649648741226144 1105 18-19 0.21974689076566278 1105 20-21 0.19439127267978762 1105 22-23 0.19675854040517748 1105 24-25 0.17397358111460193 1105 26-27 0.09890159398614173 1105 28-29 0.2084575828033408 1105 30-31 0.30709771975173794 1105 32-33 0.20572812804265794 1105 34-35 0.18231026640996362 1105 36-37 0.23370897560575798 1105 38-39 0.1569267678056363 1105 40-41 0.06466466660014447 1105 42-43 0.1128349881641455 1105 44-45 0.1344829186444585 1105 46-47 0.2758745250097405 1105 48-49 0.24084662046718108 1105 50-51 0.21112079181757792 1105 52-53 0.2592888657336587 1105 54-55 0.217303747582541 1105 56-57 0.1865838121851624 1105 58-59 0.15811485856281138 1105 60-61 0.2534520987898432 1105 62-63 0.05516962201051001 1105 64-65 0.10485033882174832 1105 66-67 -0.019233797708814393 1105 68-69 0.09132470934620329 1105 70-71 -0.12492802769219225 1105 72-73 0.042315414690371256 1105 74-75 -6.05014784717639E-4 1105 76-77 0.0011809787197165633 1105 78-79 -0.15806109029336213 1105 80-81 -0.15247167491095226 1105 82-83 -0.17598335832045464 1105 84-85 0.04825968628375321 1105 86-87 0.08852977901681314 1105 88-89 0.12409641325879761 1105 90-91 0.07502444190749813 1105 92-93 0.09332214614510903 1105 94-95 0.09404426998497328 1105 96-97 0.027159899224244555 1105 98-99 0.10132614554301966 1105 100-101 0.5101611188925546 1106 1 0.24916026503452215 1106 2 0.305047194085148 1106 3 0.2765817380505773 1106 4 0.14016329903731872 1106 5 0.17540284869802036 1106 6 0.19756255596892203 1106 7 0.22746427926471569 1106 8 0.17622219986068188 1106 9 0.23927679140556535 1106 10-11 0.2306743874096 1106 12-13 0.25779121604313815 1106 14-15 0.31927969237981557 1106 16-17 0.3979851130126093 1106 18-19 0.36163482920999 1106 20-21 0.4245939143987414 1106 22-23 0.3252547632776057 1106 24-25 0.36532295127469894 1106 26-27 0.3150556153942432 1106 28-29 0.4064286762066871 1106 30-31 0.36563413559504454 1106 32-33 0.34823742249291456 1106 34-35 0.43348138307433715 1106 36-37 0.18727396585923373 1106 38-39 0.25998832028751906 1106 40-41 0.23014959513225364 1106 42-43 0.26663812505604056 1106 44-45 0.34368173583754213 1106 46-47 0.2872187530505883 1106 48-49 0.19620946051022514 1106 50-51 0.12458770739839053 1106 52-53 0.0820250586854101 1106 54-55 -0.15364578795384176 1106 56-57 0.04138217419514234 1106 58-59 0.11879278186358277 1106 60-61 0.1357805149135558 1106 62-63 0.09028270880358491 1106 64-65 -0.014369357526355486 1106 66-67 0.1567007655376642 1106 68-69 0.14797047030418042 1106 70-71 0.18194596210641123 1106 72-73 0.014359970695657864 1106 74-75 -0.03255048043926578 1106 76-77 -0.048467258102952826 1106 78-79 0.1449279308239788 1106 80-81 0.1989619049201501 1106 82-83 0.1844743221575733 1106 84-85 0.15973792408625442 1106 86-87 0.33405711111327463 1106 88-89 0.4672809942298457 1106 90-91 0.37200154493868 1106 92-93 0.439134346071981 1106 94-95 0.553764667748414 1106 96-97 0.5775477886770446 1106 98-99 0.6121423205551082 1106 100-101 0.9529924828725456 1107 1 0.1182019912296326 1107 2 0.07109598834649589 1107 3 0.1200416552192145 1107 4 0.20712340525347628 1107 5 0.15285084897921308 1107 6 0.13199132272855962 1107 7 0.06374563401398348 1107 8 0.1933159986943167 1107 9 0.16402180058024385 1107 10-11 0.2040219402492056 1107 12-13 0.14343426597867648 1107 14-15 0.12310789003554845 1107 16-17 0.21645488065408358 1107 18-19 0.32428658426422174 1107 20-21 0.18982898872344123 1107 22-23 0.2914228312780125 1107 24-25 0.3406771070867407 1107 26-27 0.3463227347780915 1107 28-29 0.27893637064453713 1107 30-31 0.36976143030857145 1107 32-33 0.3499058857744899 1107 34-35 0.1474794341468737 1107 36-37 0.14553767267808837 1107 38-39 0.20397447311520267 1107 40-41 0.14966579466933894 1107 42-43 0.2386573447898499 1107 44-45 0.3772998120814606 1107 46-47 0.3516157943938367 1107 48-49 0.3255643744542738 1107 50-51 0.32993744142106607 1107 52-53 0.266876439373128 1107 54-55 0.30045901913515394 1107 56-57 0.36397797429142287 1107 58-59 0.29073547959010426 1107 60-61 0.152975974122306 1107 62-63 0.30831489018667213 1107 64-65 0.4413302163545154 1107 66-67 0.3309447550985922 1107 68-69 0.3031763942202659 1107 70-71 0.41323637501842825 1107 72-73 0.2975701431804012 1107 74-75 0.2996197462177399 1107 76-77 0.18962711768001128 1107 78-79 0.25892207860417926 1107 80-81 0.27740147739118015 1107 82-83 0.389088353541986 1107 84-85 0.3976475246124451 1107 86-87 0.43802756532639364 1107 88-89 0.503345667705851 1107 90-91 0.6435463107498798 1107 92-93 0.5969006161499024 1107 94-95 0.5211394065336812 1107 96-97 0.42375418661983133 1107 98-99 0.6150691101321151 1107 100-101 0.6813706707312086 1108 1 0.19776497482945743 1108 2 0.14541589370956132 1108 3 0.10208740099806235 1108 4 0.10810260289157014 1108 5 0.1866243351595145 1108 6 0.1818834737277939 1108 7 0.12677866339818422 1108 8 0.14948694462841416 1108 9 0.26284478623207974 1108 10-11 0.2334681243560084 1108 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-2.0012765095755327 2316 90-91 -2.22787261364736 2316 92-93 -2.4385756197696793 2316 94-95 -2.6855844653324006 2316 96-97 -3.1001820787571788 2316 98-99 -3.4326908850298814 2316 100-101 -4.381286842726073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10182.0 3 2580.0 4 2491.0 5 2903.0 6 3360.0 7 4091.0 8 5300.0 9 6270.0 10 7820.0 11 9920.0 12 13035.0 13 19261.0 14 18164.0 15 17060.0 16 21136.0 17 27928.0 18 24240.0 19 17737.0 20 17997.0 21 19172.0 22 21400.0 23 23975.0 24 27316.0 25 31458.0 26 36977.0 27 42853.0 28 51180.0 29 61839.0 30 73196.0 31 90177.0 32 112259.0 33 143472.0 34 193356.0 35 274061.0 36 422054.0 37 665994.0 38 634825.0 39 103408.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8435522457288 19.5293175368803 14.37081269219862 23.256317525192284 2 14.9780236039816 20.646239270429085 40.73758912274304 23.63814800284628 3 18.718309465694176 24.998871826955753 30.435315162430825 25.84750354491925 4 12.605588483087422 16.90573241993206 37.79057557544034 32.698103521540176 5 13.525834046429713 37.51620778262182 34.65878683653252 14.29917133441595 6 31.31545149483264 36.391583654515564 17.898762215463652 14.394202635188147 7 27.80662475617191 33.13725779129359 21.57328288847179 17.482834564062703 8 25.37827054482808 35.60487523802595 20.410631022405674 18.6062231947403 9 26.228329533234007 17.016821234056543 21.17769207130536 35.57715716140409 10-11 24.59037403541128 26.673376092750324 27.909728800843318 20.82652107099508 12-13 25.51930190793412 24.281769482263734 28.223441649142732 21.975486960659413 14-15 22.578738694926816 25.52045244239931 26.591322026571568 25.30948683610231 16-17 21.858490657263225 28.71821154092034 27.31644415915676 22.106853642659676 18-19 21.741503110654822 27.60162741404252 29.38715310611103 21.26971636919162 20-21 22.559732647041443 26.819388615854358 29.113395509986972 21.50748322711723 22-23 22.29806972190399 26.689104643654076 29.034283677154747 21.97854195728719 24-25 22.226426115266037 26.89911465398292 29.072479586124 21.80197964462704 26-27 22.14933320393788 26.946920429167044 29.074298377724983 21.82944798917009 28-29 21.796748340721468 27.099868092980095 29.269662567686606 21.833720998611835 30-31 22.045351087488037 26.917303622298938 29.101752462805553 21.935592827407476 32-33 21.815581444047265 27.076193306833485 29.02703635449207 22.08118889462718 34-35 22.31730487103882 27.023534225597164 28.892719288496004 21.76644161486801 36-37 22.3725299643667 26.822435096487574 28.80324556126305 22.001789377882673 38-39 22.061006081052007 27.103148084543417 28.98779764250311 21.848048191901462 40-41 22.187140159401725 26.893217309688506 29.010161139211878 21.909481391697895 42-43 22.001185272092115 27.00557491170987 28.762047606115853 22.231192210082153 44-45 22.12747178708747 27.112561511730554 28.596061799323873 22.163904901858103 46-47 22.300166274590126 27.23454711265046 28.19851097796326 22.26677563479616 48-49 22.350049171792747 27.279122235291847 28.12685165545036 22.24397693746505 50-51 22.166384745596957 27.43018481538052 28.14111643211636 22.262314006906166 52-53 22.366242990775305 27.49406999267436 27.91930901705106 22.220377999499284 54-55 22.162925360297844 27.265937891095792 28.060291335422573 22.510845413183787 56-57 22.235935334759024 27.70718968519001 27.827708824156623 22.22916615589434 58-59 22.374077059010094 27.23331020022194 28.005291063212912 22.387321677555057 60-61 22.35876003325605 27.2641043481745 28.210900063106358 22.166235555463093 62-63 22.23701741293187 27.20706023621823 28.299827469980748 22.256094880869153 64-65 22.091140911372086 27.17025586799667 28.262621266582915 22.47598195404833 66-67 22.05947996574024 27.37125472896232 27.92534507789065 22.643920227406788 68-69 21.980606223320827 27.26188850545715 28.09743023586838 22.660075035353643 70-71 22.079636534817553 27.21506653658729 28.043111345156607 22.66218558343855 72-73 22.21257970504697 27.039469522703115 27.985716732158643 22.762234040091272 74-75 22.007512632905073 27.45316869390711 27.81056489457846 22.728753778609363 76-77 22.168407640954133 27.112044498192184 27.904167232251936 22.81538062860175 78-79 22.097778597509418 27.253683279237727 27.85920348300958 22.789334640243275 80-81 22.091949375552346 27.379780969524205 27.83146443919837 22.696805215725078 82-83 22.15726470628153 27.22136505582446 27.893846539179528 22.727523698714478 84-85 22.074106636743288 26.99307829051128 27.88063039613772 23.052184676607705 86-87 22.100257855620285 26.909583657021642 28.143647743219663 22.846510744138406 88-89 22.232358131673895 26.841751219844017 27.94056332861895 22.98532731986313 90-91 22.110968907626287 26.87263271308205 28.112677612083974 22.903720767207684 92-93 22.248498398306957 26.892119960275114 27.93041633836446 22.92896530305347 94-95 22.267690621042206 26.633896300474465 27.913736415728728 23.184676662754608 96-97 22.26526010095517 26.49928948159695 27.954390442165995 23.281059975281888 98-99 22.086324497538413 26.545276699081555 27.8485988777445 23.51979992563553 100-101 22.396133796242882 26.029576810428175 28.287803337070255 23.28648605625869 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3549.0 1 2058.0 2 591.0 3 635.5 4 578.5 5 578.0 6 959.0 7 1787.0 8 2876.0 9 3552.0 10 3459.5 11 3450.0 12 3855.5 13 4179.0 14 4473.5 15 4836.0 16 5154.0 17 5331.0 18 5386.5 19 5614.5 20 6106.0 21 6715.0 22 7715.5 23 9326.5 24 11260.0 25 14163.0 26 18441.0 27 22951.5 28 26745.0 29 31452.5 30 37770.5 31 44456.5 32 51202.0 33 58178.5 34 66672.5 35 74231.0 36 82848.5 37 92845.0 38 97111.5 39 97445.0 40 97944.5 41 101310.0 42 104991.0 43 109278.0 44 113814.0 45 116118.0 46 117301.5 47 120661.0 48 135087.5 49 137650.0 50 125771.0 51 119097.5 52 112885.5 53 109129.5 54 104630.0 55 96387.5 56 87446.0 57 78396.0 58 69777.5 59 61026.0 60 51366.0 61 41029.0 62 32700.0 63 26176.0 64 19976.0 65 14961.5 66 10784.0 67 8160.5 68 5984.5 69 3743.0 70 2512.0 71 1731.5 72 1179.5 73 819.0 74 563.5 75 422.0 76 289.0 77 159.5 78 94.5 79 66.0 80 50.5 81 39.0 82 27.0 83 22.0 84 23.0 85 17.5 86 16.0 87 15.0 88 14.5 89 14.0 90 11.0 91 9.0 92 6.5 93 4.5 94 4.0 95 2.5 96 0.0 97 0.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28431536124919643 2 0.08826964505665451 3 0.09155138655111665 4 0.10673327477316112 5 0.10860417412981711 6 0.08305566324302308 7 0.09342228590777263 8 0.09020188537582381 9 0.09486379852683545 10-11 0.0876562354315214 12-13 0.09244083050755966 14-15 0.10115124718444982 16-17 0.08997185676639889 18-19 0.10035381467177677 20-21 0.10263876552539762 22-23 0.11602643059392774 24-25 0.12952144234685614 26-27 0.131760387478592 28-29 0.12278927171102028 30-31 0.11536701524690965 32-33 0.11010702771139323 34-35 0.3824915717517507 36-37 0.5862355787397131 38-39 0.5676646023388082 40-41 0.5298172284680953 42-43 0.5572979796740586 44-45 0.5799021243602137 46-47 0.5685540462952512 48-49 0.5912195319439196 50-51 0.5831378601327909 52-53 0.5861282320553148 54-55 0.5757156036686804 56-57 0.5467166636405125 58-59 0.5409506131642613 60-61 0.48893347695297357 62-63 0.5649349295069659 64-65 0.5985497769642604 66-67 0.6111860152420023 68-69 0.6131489260424283 70-71 0.6096831616604262 72-73 0.6014174669617576 74-75 0.5987798055736853 76-77 0.5822484161763478 78-79 0.5585247989243248 80-81 0.5532494761481801 82-83 0.5678946309482331 84-85 0.578184577409841 86-87 0.5684313643702246 88-89 0.5816196713105865 90-91 0.5953753821541965 92-93 0.6034263834840685 94-95 0.6020462118275189 96-97 0.5987031293705436 98-99 0.6032576958371569 100-101 0.5808375740385417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3260464.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.69138890464706 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84090255208642 29.389137433980316 2 14.415577301442827 12.308420337217967 3 6.09948830490956 7.811868820327214 4 3.1699878707910503 5.413247400598192 5 1.8549274390753114 3.959471434573257 6 1.1679665169040214 2.9917267680453365 7 0.837328736877824 2.502270872296165 8 0.5998453414206126 2.048658460258254 9 0.43619959480525033 1.6759769887692364 >10 2.3238892382997247 17.763351093049046 >50 0.16240098130757544 4.764677395426434 >100 0.08593356456904686 6.647468380216425 >500 0.004470890463783164 1.3209948941909542 >1k 8.653336376981404E-4 0.6687478926894694 >5k 1.4422227294969006E-4 0.40768900901747657 >10k+ 7.211113647484503E-5 0.326292819344275 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10599 0.3250764308392916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8028 0.2462226235284303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5215 0.15994655975345842 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4033 0.1236940509080916 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.134096251331099E-5 0.0 10-11 3.0670481256655495E-5 0.0 0.0 9.201144376996648E-5 0.0 12-13 3.0670481256655495E-5 0.0 0.0 1.0734668439829422E-4 0.0 14-15 6.134096251331099E-5 0.0 0.0 1.2268192502662198E-4 0.0 16-17 6.134096251331099E-5 0.0 0.0 1.8402288753993295E-4 0.0 18-19 6.134096251331099E-5 0.0 0.0 1.8402288753993295E-4 0.0 20-21 6.134096251331099E-5 0.0 0.0 2.606990906815717E-4 0.0 22-23 6.134096251331099E-5 0.0 0.0 4.600572188498324E-4 0.0 24-25 6.134096251331099E-5 0.0 0.0 7.360915501597319E-4 0.0 26-27 6.134096251331099E-5 0.0 0.0 0.0014261773784344806 0.0 28-29 7.667620314163873E-5 0.0 0.0 0.004155850210276819 0.0 30-31 9.201144376996648E-5 0.0 0.0 0.013234312662246846 0.0 32-33 9.201144376996648E-5 0.0 0.0 0.02947433248764593 0.0 34-35 9.201144376996648E-5 0.0 0.0 0.05437876326805019 0.0 36-37 1.2268192502662198E-4 0.0 0.0 0.09402036029227742 0.0 38-39 1.5335240628327747E-4 0.0 0.0 0.15043871056389518 0.0 40-41 1.8402288753993298E-4 0.0 0.0 0.21461669259344682 0.0 42-43 2.1469336879658847E-4 0.0 0.0 0.2833799115708684 0.0 44-45 2.1469336879658847E-4 0.0 0.0 0.36611353476069664 0.0 46-47 2.1469336879658847E-4 0.0 0.0 0.4659306160104819 0.0 48-49 2.4536385005324396E-4 0.0 0.0 0.5739060452745376 0.0 50-51 2.4536385005324396E-4 0.0 0.0 0.693612933619264 0.0 52-53 2.4536385005324396E-4 0.0 0.0 0.816064830036461 0.0 54-55 2.4536385005324396E-4 0.0 0.0 0.9512449761751701 0.0 56-57 2.4536385005324396E-4 0.0 0.0 1.113599168707276 0.0 58-59 2.4536385005324396E-4 0.0 0.0 1.29430964427149 0.0 60-61 2.4536385005324396E-4 0.0 0.0 1.4964127805122216 0.0 62-63 2.4536385005324396E-4 0.0 0.0 1.7024264031131766 0.0 64-65 2.4536385005324396E-4 0.0 0.0 1.9234685615298925 0.0 66-67 2.4536385005324396E-4 0.0 0.0 2.1749511725938393 0.0 68-69 2.4536385005324396E-4 0.0 0.0 2.4655693177412785 0.0 70-71 2.4536385005324396E-4 0.0 0.0 2.785401096285682 3.0670481256655495E-5 72-73 2.606990906815717E-4 0.0 0.0 3.1195099838550586 3.0670481256655495E-5 74-75 2.7603433130989947E-4 0.0 0.0 3.4653196600238494 3.0670481256655495E-5 76-77 2.7603433130989947E-4 0.0 0.0 3.8335341227506268 3.0670481256655495E-5 78-79 2.7603433130989947E-4 0.0 0.0 4.257522855642632 3.0670481256655495E-5 80-81 2.7603433130989947E-4 0.0 0.0 4.721260532243264 3.0670481256655495E-5 82-83 2.7603433130989947E-4 0.0 0.0 5.211988232349752 3.0670481256655495E-5 84-85 3.3737529382321045E-4 0.0 0.0 5.704893536625462 3.0670481256655495E-5 86-87 3.680457750798659E-4 0.0 3.0670481256655495E-5 6.220111616015389 3.0670481256655495E-5 88-89 3.680457750798659E-4 0.0 3.0670481256655495E-5 6.79380296792113 3.0670481256655495E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17830 0.0 26.085964 1 GGTATCA 13190 0.0 24.910225 1 GTACATG 10990 0.0 23.68448 1 ATCAACG 20615 0.0 22.294157 3 TACATGG 11430 0.0 22.071716 2 TCAACGC 20865 0.0 22.049719 4 TATCAAC 21005 0.0 21.970356 2 CAACGCA 20980 0.0 21.929192 5 GAGTACT 9295 0.0 21.616074 12-13 AACGCAG 21340 0.0 21.490402 6 ACATGGG 11515 0.0 21.333326 3 ACGCAGA 23085 0.0 19.681726 7 GTACTTT 10145 0.0 19.525187 14-15 CGCAGAG 23045 0.0 19.203337 8 CATGGGG 5685 0.0 18.816252 4 AGTACTT 10240 0.0 18.44257 12-13 GTGTAGC 1440 0.0 18.10879 1 GCAGAGT 24225 0.0 17.66254 9 AGAGTAC 17815 0.0 17.29592 10-11 CATGGGA 6625 0.0 16.503698 4 >>END_MODULE