FastQCFastQC Report
Fri 27 May 2016
SRR939038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260464
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104500.3205065291320499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87360.2679373242581424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54670.16767552103013558No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43160.13237379710372513No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG550.002593450734.509714
ATCGCGT550.002593450734.509718
GTACATG114100.024.3001941
GTATCAA191550.024.0749591
GGTATCA144350.023.9602991
TACATGG117550.022.766722
ACATGGG119900.022.0038973
GAGTACT100800.021.74930812-13
ATCAACG223650.020.3679033
CAACGCA225250.020.2232255
GTATAGG8250.020.210941
TCAACGC226300.020.1293934
AACGCAG228300.019.953056
TATCAAC229600.019.943412
GTACTTT110800.019.59364914-15
AGTACTT109050.019.53820412-13
ACGCAGA249300.018.0058177
GTGTAGC12250.017.8893341
CATGGGG60950.017.8281294
ACCGTAT1600.001199954917.794073
Produced by FastQC (version 0.11.5)