##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939038_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3260464 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1435050348662 33.0 31.0 34.0 30.0 34.0 2 32.377409473007525 34.0 31.0 34.0 30.0 34.0 3 32.474556995568726 34.0 31.0 34.0 30.0 34.0 4 36.05511546822783 37.0 35.0 37.0 35.0 37.0 5 35.96537302666123 37.0 35.0 37.0 35.0 37.0 6 36.00410309698252 37.0 35.0 37.0 35.0 37.0 7 35.941897226897765 37.0 35.0 37.0 35.0 37.0 8 35.910147758110504 37.0 35.0 37.0 35.0 37.0 9 37.59220466780188 39.0 37.0 39.0 35.0 39.0 10-11 37.509626543952024 39.0 37.0 39.0 34.5 39.0 12-13 37.43714038860727 39.0 37.0 39.0 34.0 39.0 14-15 38.70277221278934 40.0 38.0 41.0 34.0 41.0 16-17 38.68963711913396 40.0 38.0 41.0 34.0 41.0 18-19 38.78428975139735 40.0 38.0 41.0 34.0 41.0 20-21 38.78625910299884 40.0 38.0 41.0 34.0 41.0 22-23 38.72719312343274 40.0 38.0 41.0 34.0 41.0 24-25 38.665291811226865 40.0 38.0 41.0 34.0 41.0 26-27 38.513342732813484 40.0 38.0 41.0 34.0 41.0 28-29 38.33291227874315 40.0 38.0 41.0 34.0 41.0 30-31 38.170583082653266 40.0 38.0 41.0 33.0 41.0 32-33 38.02691610764603 40.0 37.0 41.0 33.0 41.0 34-35 38.00654416058573 40.0 37.0 41.0 33.0 41.0 36-37 37.90952959456077 40.0 37.0 41.0 33.0 41.0 38-39 37.79067273860407 40.0 37.0 41.0 33.0 41.0 40-41 37.63646462589374 40.0 37.0 41.0 33.0 41.0 42-43 37.47765701446174 40.0 37.0 41.0 32.0 41.0 44-45 37.23836039901069 40.0 37.0 41.0 31.5 41.0 46-47 36.942677943998156 40.0 36.0 41.0 31.0 41.0 48-49 36.67431706039386 40.0 36.0 41.0 30.5 41.0 50-51 36.41503233895544 39.0 36.0 41.0 30.0 41.0 52-53 36.19001007218604 39.0 35.0 41.0 29.5 41.0 54-55 35.94013505439716 39.0 35.0 40.0 29.0 41.0 56-57 35.670161976945614 38.5 35.0 40.0 28.0 41.0 58-59 35.36777679495924 38.0 34.5 40.0 28.0 41.0 60-61 34.96542301954568 38.0 34.0 40.0 27.0 41.0 62-63 34.656995752751754 37.5 34.0 40.0 26.0 41.0 64-65 34.396590178575806 37.0 34.0 40.0 26.0 41.0 66-67 33.942105479465496 36.5 33.0 39.0 26.0 41.0 68-69 33.5268374378616 36.0 33.0 39.0 25.5 40.0 70-71 33.07139719990774 35.0 32.5 38.5 24.5 40.0 72-73 32.5572404725217 35.0 32.0 37.5 23.5 39.5 74-75 32.08180476766497 35.0 31.5 37.0 22.0 39.0 76-77 30.085531077785248 33.0 29.0 35.0 20.0 37.5 78-79 31.082251636576878 34.0 31.0 36.0 20.0 38.0 80-81 31.081646354629278 34.0 31.0 36.0 20.0 37.0 82-83 30.762303616908515 34.0 31.0 35.0 19.5 37.0 84-85 30.354975702844747 34.0 30.5 35.0 18.0 36.5 86-87 29.774942155472353 34.0 30.0 35.0 10.5 36.0 88-89 29.19814235029125 34.0 29.0 35.0 6.0 35.5 90-91 28.729007129046664 34.0 29.0 35.0 2.0 35.0 92-93 28.367183474499335 33.0 29.0 35.0 2.0 35.0 94-95 27.963095590075522 33.0 28.0 35.0 2.0 35.0 96-97 27.32678799704582 33.0 27.0 35.0 2.0 35.0 98-99 26.405675081828843 33.0 25.5 34.0 2.0 35.0 100-101 22.013158863278356 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14491589044632036 1101 2 0.0982484226164928 1101 3 0.13622195517869784 1101 4 0.05059908948403091 1101 5 0.10306977504692583 1101 6 0.07191980705534462 1101 7 0.07940525649735264 1101 8 0.13376074085677914 1101 9 0.08978934986212295 1101 10-11 0.222656305104735 1101 12-13 0.18731110547103214 1101 14-15 0.21127420368584637 1101 16-17 0.15125994796773767 1101 18-19 0.17432819999476123 1101 20-21 0.22115498249599597 1101 22-23 0.2695582020815408 1101 24-25 0.18684587976947142 1101 26-27 0.13557997817427037 1101 28-29 0.14197723759890835 1101 30-31 0.11883043123519599 1101 32-33 0.1520472283107921 1101 34-35 0.24870909264613061 1101 36-37 0.11428565680463976 1101 38-39 0.10097331207965254 1101 40-41 0.2489606968322704 1101 42-43 0.16857835183681402 1101 44-45 0.2758471764638202 1101 46-47 0.1940162320443264 1101 48-49 -0.01680435631606514 1101 50-51 0.08153619995611194 1101 52-53 0.15673805707685773 1101 54-55 0.1972144440526904 1101 56-57 -0.007335870669940903 1101 58-59 0.08914447044861618 1101 60-61 0.14330995438588445 1101 62-63 0.14435900540296842 1101 64-65 0.1673403061634815 1101 66-67 0.23834893180886496 1101 68-69 0.09267296575701067 1101 70-71 0.07752675525789954 1101 72-73 0.0908643317425728 1101 74-75 0.024257020148972686 1101 76-77 -0.13738531504038676 1101 78-79 0.1464029725499536 1101 80-81 0.2596542382374345 1101 82-83 0.24832227449024202 1101 84-85 0.15866875089824006 1101 86-87 0.34892656987837256 1101 88-89 0.4821099070866701 1101 90-91 0.5957695022167648 1101 92-93 0.6157322436058728 1101 94-95 0.4895311266742013 1101 96-97 0.3171525058865967 1101 98-99 0.4262411211369752 1101 100-101 0.878603410815419 1102 1 0.19594201340041195 1102 2 0.15335361578517848 1102 3 0.17662649416053 1102 4 0.1273013528453717 1102 5 0.16458392178117975 1102 6 0.18788876689243494 1102 7 0.13477144047620015 1102 8 0.1834654566929501 1102 9 0.17752000734603968 1102 10-11 0.19935263245753987 1102 12-13 0.23452802077257928 1102 14-15 0.31247040618890054 1102 16-17 0.2689211541517693 1102 18-19 0.16923694354176888 1102 20-21 0.28419829664698426 1102 22-23 0.2841859465983134 1102 24-25 0.3098782058524918 1102 26-27 0.2529253499910027 1102 28-29 0.2836020134661936 1102 30-31 0.21402319988636265 1102 32-33 0.26355047085545635 1102 34-35 0.28848776985824287 1102 36-37 0.2768669623781008 1102 38-39 0.2814105507259086 1102 40-41 0.2757333857731652 1102 42-43 0.25967140328716454 1102 44-45 0.2786856531549873 1102 46-47 0.4536246619507196 1102 48-49 0.37317124152709624 1102 50-51 0.38096121365087043 1102 52-53 0.31445328739075507 1102 54-55 0.3213275108205238 1102 56-57 0.31967464574344007 1102 58-59 0.3397458139221925 1102 60-61 0.2247186014727518 1102 62-63 0.3352481356035142 1102 64-65 0.3701885579121864 1102 66-67 0.411946694965998 1102 68-69 0.35899021444286205 1102 70-71 0.4005344985229442 1102 72-73 0.36477063829999423 1102 74-75 0.47260051889693244 1102 76-77 0.30174075610267437 1102 78-79 0.26651439530150967 1102 80-81 0.496431456610928 1102 82-83 0.5035543498414192 1102 84-85 0.5406463837599844 1102 86-87 0.4912514076220944 1102 88-89 0.6048746288717588 1102 90-91 0.6956902694071552 1102 92-93 0.6894961827411379 1102 94-95 0.6216405453776588 1102 96-97 0.5081539040746925 1102 98-99 0.6982867793661214 1102 100-101 1.051673869301105 1103 1 0.16108291125448915 1103 2 0.15629911583709344 1103 3 0.15349560194157164 1103 4 0.10285367026889247 1103 5 0.09629126707974933 1103 6 0.09502306182883302 1103 7 0.09620455928060778 1103 8 0.14202047402424256 1103 9 0.16236143477721754 1103 10-11 0.17609364007807926 1103 12-13 0.08589100969489039 1103 14-15 0.2594349574295549 1103 16-17 0.22618504401842188 1103 18-19 0.20010458184091817 1103 20-21 0.2691768250027664 1103 22-23 0.21784485042739732 1103 24-25 0.22729035544818998 1103 26-27 0.23574155192357438 1103 28-29 0.30370966689091716 1103 30-31 0.3213307381013024 1103 32-33 0.33315552268431503 1103 34-35 0.29012944493901927 1103 36-37 0.24800799460072653 1103 38-39 0.3396510127822765 1103 40-41 0.33213678116140954 1103 42-43 0.43282001308165263 1103 44-45 0.4609182305419992 1103 46-47 0.5223238511640247 1103 48-49 0.38897010059452697 1103 50-51 0.3909115583640599 1103 52-53 0.4604591822483357 1103 54-55 0.5471835772444535 1103 56-57 0.46197158287985474 1103 58-59 0.42404059494700164 1103 60-61 0.28896996666900776 1103 62-63 0.4031103073314526 1103 64-65 0.4090542323303268 1103 66-67 0.42359191570491106 1103 68-69 0.5596061024416414 1103 70-71 0.4728054373808632 1103 72-73 0.5215589663662712 1103 74-75 0.6028697563961245 1103 76-77 0.544299294018554 1103 78-79 0.6005468415559676 1103 80-81 0.5499645726186664 1103 82-83 0.5995714474357392 1103 84-85 0.590687149720722 1103 86-87 0.46931750987656073 1103 88-89 0.6238772777752004 1103 90-91 0.7960121967364842 1103 92-93 0.7622790936332535 1103 94-95 0.7248590578920968 1103 96-97 0.5987246821725343 1103 98-99 0.7454790713689086 1103 100-101 0.976813496333012 1104 1 -0.049045535727138656 1104 2 -0.020482261543634195 1104 3 -2.3088105316304564E-4 1104 4 0.013943850919261536 1104 5 0.05853706954651017 1104 6 -0.007710921878128829 1104 7 -0.02319444803212889 1104 8 -0.0026292114688359902 1104 9 -0.049086612890484105 1104 10-11 -0.02480433795447823 1104 12-13 0.05265046056722866 1104 14-15 0.07627681546408382 1104 16-17 0.008573220496529643 1104 18-19 0.04509066316575172 1104 20-21 -0.010043672794552094 1104 22-23 -0.02816355591771469 1104 24-25 -0.04433862664216548 1104 26-27 0.043183082626946145 1104 28-29 -0.11778171581165964 1104 30-31 -0.18507239552215538 1104 32-33 -0.030897671133651272 1104 34-35 0.015016042407758334 1104 36-37 -0.07441710270885693 1104 38-39 0.14766702301826484 1104 40-41 0.005837576013007606 1104 42-43 -0.20772764208944494 1104 44-45 -0.019378719296462066 1104 46-47 -0.11350839329335827 1104 48-49 -0.13620643098190044 1104 50-51 -0.18524321755445783 1104 52-53 -0.21698256985053632 1104 54-55 -0.2179137678476195 1104 56-57 -0.254160023791421 1104 58-59 -0.14013318631258187 1104 60-61 -0.09558904483770903 1104 62-63 -0.006795564077030747 1104 64-65 -0.049922122630974286 1104 66-67 -0.06431633967353889 1104 68-69 -0.02227357453815415 1104 70-71 -0.1590491028558887 1104 72-73 -0.19667715067424751 1104 74-75 -0.2780039078162524 1104 76-77 -0.280292450602996 1104 78-79 -0.12813747186318736 1104 80-81 -0.006745465285913355 1104 82-83 0.0835206396763688 1104 84-85 -0.04546047888290872 1104 86-87 -0.1995989492492356 1104 88-89 -0.026419037117697997 1104 90-91 0.17654922492389247 1104 92-93 0.06775182313892358 1104 94-95 0.17331489742798567 1104 96-97 0.06625536215746308 1104 98-99 0.2189519750668829 1104 100-101 0.7468218007526524 1105 1 -0.08281345035285881 1105 2 -0.01676561492168105 1105 3 0.018630735858309322 1105 4 -0.008255129879955803 1105 5 -0.018387628242955145 1105 6 0.004838636111607286 1105 7 -0.021153777252784778 1105 8 0.08816321389836901 1105 9 -0.017763775207285448 1105 10-11 -0.060674313673892755 1105 12-13 -0.08716101646347596 1105 14-15 -0.005101820544439306 1105 16-17 0.024804576480931928 1105 18-19 0.021120480623778803 1105 20-21 0.03364495715192817 1105 22-23 -0.0060330567232824706 1105 24-25 0.017693956013907552 1105 26-27 0.04892398875609416 1105 28-29 0.08694951212429203 1105 30-31 0.018948082503754904 1105 32-33 -0.030010449395888372 1105 34-35 0.0852832341270755 1105 36-37 0.1221139595745484 1105 38-39 0.09113140610030968 1105 40-41 0.17518670901156952 1105 42-43 0.17217770741797978 1105 44-45 -0.02275579441065645 1105 46-47 0.11841063956808995 1105 48-49 0.053146016688423 1105 50-51 0.0627779071971446 1105 52-53 0.13196990472616932 1105 54-55 0.14808090112283878 1105 56-57 0.1097184015044732 1105 58-59 0.06741713348435496 1105 60-61 -0.017335439220438786 1105 62-63 -0.016427359964573895 1105 64-65 -0.07042486675776871 1105 66-67 0.07887748028711172 1105 68-69 0.02882869564729873 1105 70-71 0.10711478456521917 1105 72-73 0.1484340731771283 1105 74-75 0.27167726776571754 1105 76-77 -0.009788288218121721 1105 78-79 0.12904863925692567 1105 80-81 0.12951550392735456 1105 82-83 0.14555180407436197 1105 84-85 0.05692580113148438 1105 86-87 -0.013306899923030358 1105 88-89 0.18829267558697538 1105 90-91 0.2908731858674969 1105 92-93 0.28511243673409936 1105 94-95 0.36784216364232236 1105 96-97 0.12271378531380961 1105 98-99 0.3709071856660522 1105 100-101 0.7407322057423293 1106 1 0.04271042047200524 1106 2 -0.03532424424219727 1106 3 -0.005996470453396796 1106 4 0.042379210873271234 1106 5 -0.0014138550426707752 1106 6 0.005500220935864775 1106 7 -0.01276308134202253 1106 8 0.04206258131016227 1106 9 -0.02548571680141265 1106 10-11 0.09987929859518374 1106 12-13 0.0935747168441523 1106 14-15 0.07364897905311807 1106 16-17 0.0032944065559945557 1106 18-19 -0.06482092152181451 1106 20-21 0.006640566236150391 1106 22-23 0.1836109700141435 1106 24-25 0.0802951753055865 1106 26-27 0.1269288624176923 1106 28-29 0.18362912105237683 1106 30-31 0.16227051688099436 1106 32-33 0.10853152678706124 1106 34-35 0.13556208754999233 1106 36-37 0.10437534094455003 1106 38-39 0.08511241809805625 1106 40-41 0.10366066662712825 1106 42-43 0.25621859662621915 1106 44-45 0.2010284588349336 1106 46-47 0.27855808017880435 1106 48-49 0.1262583092610683 1106 50-51 0.15419162355131277 1106 52-53 0.3315927695119356 1106 54-55 0.2916698016333754 1106 56-57 0.18734349122269833 1106 58-59 0.220326274507471 1106 60-61 0.34945150895767796 1106 62-63 0.27458572117257063 1106 64-65 0.1879391427091477 1106 66-67 0.20779033032761873 1106 68-69 0.18748219328416127 1106 70-71 0.18280012829059444 1106 72-73 0.3588893439091052 1106 74-75 0.4059442349668885 1106 76-77 0.25836367156261986 1106 78-79 0.4685349570773383 1106 80-81 0.4740340087854804 1106 82-83 0.5504778627096591 1106 84-85 0.46974557044908494 1106 86-87 0.39136016080018265 1106 88-89 0.4033848155131601 1106 90-91 0.7573205590053185 1106 92-93 0.7856043435721247 1106 94-95 0.8759638044364166 1106 96-97 0.7345205252739326 1106 98-99 0.8375722325585144 1106 100-101 1.1062707660604651 1107 1 -0.040857029487689545 1107 2 0.03018008928616922 1107 3 0.05370341491409647 1107 4 -0.031053272558615674 1107 5 -0.10399354289192075 1107 6 -0.008772429665683035 1107 7 -0.00828157741319302 1107 8 -0.026381164382378586 1107 9 -0.1268804350060364 1107 10-11 0.028636494198089224 1107 12-13 0.05199484664707654 1107 14-15 0.06809586602211937 1107 16-17 0.06695525977686145 1107 18-19 0.07775173457541484 1107 20-21 -0.03031302866695995 1107 22-23 -0.009437712974239787 1107 24-25 -0.10070097597048999 1107 26-27 -0.021551358667892373 1107 28-29 0.054077343296910385 1107 30-31 0.04692405307937264 1107 32-33 0.12487039781121467 1107 34-35 0.04325480678345173 1107 36-37 0.16036059310208373 1107 38-39 0.1690617984164149 1107 40-41 0.08844972086328085 1107 42-43 -0.018105614330998776 1107 44-45 0.10858058186671116 1107 46-47 -0.13369872383261594 1107 48-49 0.06931801273902494 1107 50-51 0.1369052817149523 1107 52-53 0.20172678643280761 1107 54-55 -0.11525546430026168 1107 56-57 0.13899170010670758 1107 58-59 0.05443232055195324 1107 60-61 0.002157044000988151 1107 62-63 0.05168204646557939 1107 64-65 0.059536360224875295 1107 66-67 0.11242678136877515 1107 68-69 0.052803751750325034 1107 70-71 0.061735972340663636 1107 72-73 0.10552945394699975 1107 74-75 0.016157917890218698 1107 76-77 -0.3189899214762342 1107 78-79 -0.030261139397289583 1107 80-81 0.1739927814738529 1107 82-83 0.2553905153774245 1107 84-85 0.26086685234466245 1107 86-87 0.3384838033137534 1107 88-89 0.4434407996001575 1107 90-91 0.7274925602038849 1107 92-93 0.7904076749253299 1107 94-95 0.8336039069007519 1107 96-97 0.5982035262804359 1107 98-99 0.4648264081588991 1107 100-101 0.936479636865176 1108 1 0.03454875077762409 1108 2 -0.002769833448141412 1108 3 0.014547522152220438 1108 4 -0.054837654904737576 1108 5 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-1.9017483939123423 2316 92-93 -2.2939055084030144 2316 94-95 -2.435776220163021 2316 96-97 -2.6239120453347056 2316 98-99 -3.011784630064902 2316 100-101 -3.7081027652577596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 30.0 5 93.0 6 173.0 7 373.0 8 886.0 9 1703.0 10 2936.0 11 4535.0 12 6351.0 13 9048.0 14 10721.0 15 14001.0 16 18544.0 17 25928.0 18 25046.0 19 17660.0 20 16442.0 21 17653.0 22 19163.0 23 21014.0 24 25168.0 25 29587.0 26 36296.0 27 43548.0 28 53635.0 29 65634.0 30 79783.0 31 97125.0 32 121375.0 33 154494.0 34 206027.0 35 294439.0 36 466590.0 37 748316.0 38 584067.0 39 42067.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.64996829696337 20.29958214812632 14.894914980739522 23.155534574170787 2 15.333983138596224 21.25323266872445 39.88116415332296 23.531620039356362 3 18.66580952895048 25.194297498760914 30.694342891073173 25.445550081215433 4 12.954076475004783 17.536062351861574 37.23721531659297 32.27264585654066 5 13.400923247640584 37.76365633301201 34.6203450017743 14.21507541757311 6 31.265273899665814 36.83932716325039 17.555476766497037 14.339922170586764 7 27.74626556220219 33.26732023417526 21.415724878422214 17.57068932520034 8 25.37476261047507 35.95804155482165 20.12618449398613 18.54101134071715 9 26.008476094200088 17.040120669941455 20.98308706981583 35.96831616604263 10-11 24.54698165659857 26.89558909406759 27.702790155020878 20.854639094312958 12-13 25.380421314266925 24.405974119021096 28.222639477080563 21.990965089631416 14-15 22.440251225109627 25.699721431808303 26.50276152860557 25.3572658144765 16-17 21.7392531200001 28.904008478775378 27.267126971378918 22.0896114298456 18-19 21.656595502971356 27.693113618184405 29.381431599919523 21.26885927892472 20-21 22.473396424557976 26.88827418428788 29.13063907468385 21.507690316470292 22-23 22.182072858341634 26.808837637833143 29.052444682720008 21.95664482110522 24-25 22.134809646725127 26.990345545910028 29.15629493225504 21.7185498751098 26-27 22.109291806319593 27.01147750749587 29.124980370891997 21.75425031529255 28-29 21.747257445566028 27.134389461131914 29.326669455635763 21.791683637666296 30-31 22.000580285505375 26.990682921203852 29.185784599983318 21.822952193307454 32-33 21.8076016174385 27.14316121877132 29.043749601283743 22.00548756250644 34-35 22.238438762742536 27.082263356038705 28.94198038055894 21.737317500659813 36-37 22.288390359770972 26.8440896346343 28.924689889893695 21.942830115701028 38-39 22.02210415887019 27.144127950135278 29.054959785522787 21.778808105471743 40-41 22.06891665462817 26.97410695033973 29.062794348990128 21.894182046041973 42-43 21.96981454059631 27.08540615940651 28.78229161427094 22.16248768572624 44-45 22.068480497993143 27.19004897028674 28.591007397299727 22.15046313442039 46-47 22.227025578048686 27.338648138356277 28.18845362544825 22.245872658146784 48-49 22.30458466967627 27.336983541857528 28.14549082596805 22.212940962498156 50-51 22.107498565762604 27.483837704901187 28.11235649383282 22.29630723550339 52-53 22.33591599232502 27.5701709940671 27.877489216258788 22.216423797349087 54-55 22.145022916983596 27.346736538112363 27.977904371893082 22.53033617301096 56-57 22.13433425426565 27.80622635305895 27.766584756034725 22.292854636640673 58-59 22.343364030452165 27.35161747214655 27.920862651811497 22.38415584558979 60-61 22.31215557094232 27.399541924209696 28.079616547470348 22.208685957377636 62-63 22.18596800946123 27.33607854587568 28.186187610107027 22.291765834556063 64-65 21.9964167539373 27.298673205229402 28.133925623491102 22.5709844173422 66-67 21.995328502346563 27.47275228073275 27.81901236056012 22.712906856360572 68-69 21.888209627880702 27.33768432520367 28.00037786705091 22.773728179864715 70-71 21.931264997865334 27.333364208284465 27.943645444329395 22.791725349520807 72-73 22.082240441851223 27.17573326986588 27.840301257735096 22.901725030547798 74-75 21.81988514518179 27.549330402053208 27.6532113220695 22.977573130695507 76-77 21.940179088399237 27.214533432480565 27.847403135298354 22.99788434382184 78-79 21.895658022644955 27.327450423812707 27.782294009605696 22.99459754393665 80-81 21.773158666987275 27.450479440963004 27.780601165969017 22.995760726080704 82-83 21.82253814181049 27.340142384642185 27.789296247405275 23.048023226142046 84-85 21.72322712350144 27.119514277722434 27.76469852143744 23.392560077338686 86-87 21.64904580747643 27.043453728331762 28.033959950037758 23.27354051415405 88-89 21.764194324395845 26.956870916494953 27.82442464725016 23.454510111859044 90-91 21.581964997078703 27.01517457991278 27.98645725252965 23.416403170478866 92-93 21.6692317412491 27.00144826012494 27.84395104500464 23.485368953621325 94-95 21.568111164545904 26.817394702103748 27.820564803046437 23.79392933030391 96-97 21.535207623852095 26.68329831006534 27.84628141225226 23.935212653830305 98-99 21.299054367721894 26.68077304334598 27.739456715363215 24.280715873568916 100-101 21.397344420535042 26.215090772579053 28.369804714432583 24.017760092453326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 920.0 1 635.0 2 389.5 3 453.0 4 423.5 5 448.0 6 739.5 7 1406.0 8 2287.5 9 2839.5 10 2768.0 11 2659.5 12 2925.5 13 3246.5 14 3574.0 15 3807.0 16 3970.5 17 4218.5 18 4453.0 19 4845.0 20 5567.5 21 6387.0 22 7590.0 23 9437.0 24 11427.5 25 14394.0 26 18966.0 27 23463.0 28 27061.5 29 31901.0 30 38149.0 31 45171.5 32 51928.0 33 58750.0 34 66932.0 35 74257.0 36 82927.0 37 92575.0 38 97489.5 39 98307.0 40 98782.5 41 102254.0 42 105541.0 43 109766.0 44 115252.5 45 117650.5 46 117890.0 47 121314.5 48 136709.5 49 139704.5 50 127173.0 51 119931.0 52 113330.0 53 109433.5 54 105257.0 55 96781.5 56 87500.0 57 78423.5 58 69981.0 59 61310.0 60 51352.5 61 40542.0 62 32182.5 63 25890.5 64 19643.0 65 14438.0 66 10230.5 67 7744.5 68 5537.5 69 3237.0 70 2092.5 71 1469.5 72 992.0 73 674.5 74 478.5 75 331.5 76 204.5 77 87.0 78 38.0 79 23.5 80 19.0 81 11.0 82 4.5 83 4.0 84 5.0 85 5.5 86 4.0 87 2.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40304079419370986 2 0.0 3 0.0 4 0.0 5 5.213981813631434E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001272824972151203 16-17 0.002683667109957356 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00231562133487749 36-37 0.007698290795420529 38-39 0.01361769367795504 40-41 0.00279101379435565 42-43 6.134096251331099E-4 44-45 5.367334219914711E-4 46-47 3.680457750798659E-4 48-49 3.3737529382321045E-4 50-51 6.287448657614377E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.8402288753993295E-4 60-61 0.0026529966287007004 62-63 0.0 64-65 3.8338101570819366E-4 66-67 0.0016408707472310691 68-69 0.0017788879128860188 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12352536326118002 78-79 7.667620314163873E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.147386997678858 88-89 0.43935464400158997 90-91 0.33889348264541486 92-93 0.0 94-95 0.0 96-97 3.8338101570819366E-4 98-99 0.0 100-101 2.4536385005324396E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3260464.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.89361198561372 #Duplication Level Percentage of deduplicated Percentage of total 1 66.77626824820936 27.307228035895314 2 15.413109565409968 12.605954441192532 3 6.42240875809184 7.87906475299245 4 3.327715839400987 5.443292813393793 5 1.983981985724961 4.0566094755341995 6 1.2733979648238962 3.1244305366067158 7 0.8885062342409522 2.5433960432893823 8 0.6293797984439394 2.0590090615320182 9 0.5001690741163749 1.8408348039706837 >10 2.5102074161128995 18.38410502254712 >50 0.17488398599460836 4.934363540142826 >100 0.09342501464062401 6.871997452724996 >500 0.00504126081552876 1.3521022606601878 >1k 0.0012791258778853682 0.8390523298465218 >5k 1.504853973982786E-4 0.437018601371893 >10k+ 7.52426986991393E-5 0.3215408282993932 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10450 0.3205065291320499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8736 0.2679373242581424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5467 0.16767552103013558 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4316 0.13237379710372513 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0670481256655495E-5 0.0 10-11 3.0670481256655495E-5 0.0 0.0 6.134096251331099E-5 0.0 12-13 3.0670481256655495E-5 0.0 0.0 7.667620314163873E-5 0.0 14-15 3.0670481256655495E-5 0.0 0.0 9.201144376996648E-5 0.0 16-17 3.0670481256655495E-5 0.0 0.0 1.5335240628327747E-4 0.0 18-19 3.0670481256655495E-5 0.0 0.0 1.5335240628327747E-4 0.0 20-21 3.0670481256655495E-5 0.0 0.0 2.1469336879658844E-4 0.0 22-23 3.0670481256655495E-5 0.0 0.0 3.987162563365214E-4 0.0 24-25 3.0670481256655495E-5 0.0 0.0 6.747505876464209E-4 0.0 26-27 3.0670481256655495E-5 0.0 0.0 0.001272824972151203 0.0 28-29 3.0670481256655495E-5 0.0 0.0 0.0039871625633652145 0.0 30-31 3.0670481256655495E-5 0.0 0.0 0.013264983143503502 0.0 32-33 3.0670481256655495E-5 0.0 0.0 0.029903719225239107 0.0 34-35 3.0670481256655495E-5 0.0 0.0 0.05513019005883825 0.0 36-37 3.0670481256655495E-5 0.0 0.0 0.09536986146757026 0.0 38-39 3.0670481256655495E-5 0.0 0.0 0.15278500238002934 0.0 40-41 3.0670481256655495E-5 0.0 0.0 0.21774508168162568 0.0 42-43 4.6005721884983245E-5 0.0 0.0 0.2874897560592603 0.0 44-45 6.134096251331099E-5 0.0 0.0 0.3715728804243813 0.0 46-47 7.667620314163873E-5 0.0 0.0 0.47260144568380447 0.0 48-49 9.201144376996648E-5 0.0 0.0 0.5816810122730998 0.0 50-51 9.201144376996648E-5 0.0 0.0 0.7026147198680923 0.0 52-53 9.201144376996648E-5 0.0 0.0 0.8257567021135641 0.0 54-55 9.201144376996648E-5 0.0 0.0 0.9617496160055746 0.0 56-57 9.201144376996648E-5 0.0 0.0 1.1252846220660617 0.0 58-59 9.201144376996648E-5 0.0 0.0 1.3069918882711173 0.0 60-61 9.201144376996648E-5 0.0 0.0 1.5110426000716464 0.0 62-63 9.201144376996648E-5 0.0 0.0 1.7189271220292572 0.0 64-65 9.201144376996648E-5 0.0 0.0 1.9407053719961331 0.0 66-67 9.201144376996648E-5 0.0 0.0 2.193644830919771 0.0 68-69 9.201144376996648E-5 0.0 0.0 2.4856278124831315 0.0 70-71 1.0734668439829422E-4 0.0 0.0 2.807575854234244 0.0 72-73 1.2268192502662198E-4 0.0 0.0 3.144751789929286 0.0 74-75 1.2268192502662198E-4 0.0 0.0 3.4943799410145306 0.0 76-77 1.2268192502662198E-4 0.0 0.0 3.865630781385717 0.0 78-79 1.2268192502662198E-4 0.0 0.0 4.2937293587661145 0.0 80-81 1.5335240628327747E-4 0.0 0.0 4.760503413011154 0.0 82-83 1.8402288753993295E-4 0.0 0.0 5.25409880311514 0.0 84-85 1.8402288753993295E-4 0.0 0.0 5.748721654341223 0.0 86-87 1.8402288753993295E-4 0.0 0.0 6.266055996937859 0.0 88-89 1.8402288753993295E-4 0.0 0.0 6.842661044562982 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 55 0.0025934507 34.50971 4 ATCGCGT 55 0.0025934507 34.50971 8 GTACATG 11410 0.0 24.300194 1 GTATCAA 19155 0.0 24.074959 1 GGTATCA 14435 0.0 23.960299 1 TACATGG 11755 0.0 22.76672 2 ACATGGG 11990 0.0 22.003897 3 GAGTACT 10080 0.0 21.749308 12-13 ATCAACG 22365 0.0 20.367903 3 CAACGCA 22525 0.0 20.223225 5 GTATAGG 825 0.0 20.21094 1 TCAACGC 22630 0.0 20.129393 4 AACGCAG 22830 0.0 19.95305 6 TATCAAC 22960 0.0 19.94341 2 GTACTTT 11080 0.0 19.593649 14-15 AGTACTT 10905 0.0 19.538204 12-13 ACGCAGA 24930 0.0 18.005817 7 GTGTAGC 1225 0.0 17.889334 1 CATGGGG 6095 0.0 17.828129 4 ACCGTAT 160 0.0011999549 17.79407 3 >>END_MODULE