##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939037_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715964 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.966908950729366 33.0 31.0 34.0 30.0 34.0 2 32.05439239961786 33.0 31.0 34.0 30.0 34.0 3 32.06979680542597 34.0 31.0 34.0 30.0 34.0 4 35.62039990837528 37.0 35.0 37.0 33.0 37.0 5 35.67372242179774 37.0 35.0 37.0 35.0 37.0 6 35.79399103865558 37.0 35.0 37.0 35.0 37.0 7 35.749506958450425 37.0 35.0 37.0 35.0 37.0 8 35.75407981406887 37.0 35.0 37.0 35.0 37.0 9 37.37064852422748 39.0 37.0 39.0 35.0 39.0 10-11 37.28666525132549 39.0 37.0 39.0 34.0 39.0 12-13 37.232133878239694 39.0 37.0 39.0 34.0 39.0 14-15 38.40929432206089 40.0 38.0 41.0 34.0 41.0 16-17 38.3315138749993 40.0 38.0 41.0 34.0 41.0 18-19 38.27662145024051 40.0 38.0 41.0 34.0 41.0 20-21 38.36554351894788 40.0 38.0 41.0 34.0 41.0 22-23 38.29362160667296 40.0 38.0 41.0 34.0 41.0 24-25 38.19866571503595 40.0 38.0 41.0 34.0 41.0 26-27 38.04991521920097 40.0 38.0 41.0 34.0 41.0 28-29 37.93658968886704 40.0 37.5 41.0 33.5 41.0 30-31 37.747577392159386 40.0 37.0 41.0 33.0 41.0 32-33 37.50070394600846 40.0 36.0 41.0 33.0 41.0 34-35 37.1745506757323 40.0 35.5 41.0 32.5 41.0 36-37 36.886517338860614 39.5 35.0 41.0 31.5 41.0 38-39 36.65127785754591 39.0 35.0 41.0 31.0 41.0 40-41 36.56467992804107 39.0 35.0 41.0 31.0 41.0 42-43 36.35743137923136 39.0 35.0 41.0 30.5 41.0 44-45 36.18882792989592 39.0 35.0 41.0 30.0 41.0 46-47 35.918092948807484 39.0 35.0 41.0 29.5 41.0 48-49 35.64048262203127 39.0 35.0 41.0 27.5 41.0 50-51 34.554372147202926 38.0 34.0 40.0 25.5 40.5 52-53 34.73509492097368 38.5 34.5 39.5 26.0 40.5 54-55 35.301462084685824 39.0 35.0 40.5 27.0 41.0 56-57 35.25267611220676 39.0 35.0 41.0 26.0 41.0 58-59 35.11593809185937 39.0 35.0 41.0 26.0 41.0 60-61 34.82040018771893 39.0 35.0 40.5 25.5 41.0 62-63 34.498906369593996 38.0 35.0 40.0 23.5 41.0 64-65 34.13977378750887 38.0 34.0 40.0 22.0 41.0 66-67 33.70962366822913 37.0 34.0 40.0 21.5 41.0 68-69 33.243725243168655 37.0 33.5 39.0 19.5 41.0 70-71 32.73194951142795 36.0 33.0 39.0 16.0 40.5 72-73 32.229685431111065 36.0 32.5 38.5 10.5 40.0 74-75 31.710106932750808 35.0 32.0 37.5 6.0 40.0 76-77 31.23531211066478 35.0 31.5 37.0 2.0 39.0 78-79 30.76368783905336 35.0 31.0 36.5 2.0 39.0 80-81 30.21614843763094 35.0 31.0 36.0 2.0 38.5 82-83 29.656887078121244 34.5 30.0 35.5 2.0 37.0 84-85 29.148033281002956 34.0 29.5 35.0 2.0 37.0 86-87 28.691668854858627 34.0 29.0 35.0 2.0 36.0 88-89 28.10963260722606 34.0 29.0 35.0 2.0 36.0 90-91 27.57362940036091 34.0 27.5 35.0 2.0 36.0 92-93 27.022801425770012 34.0 26.0 35.0 2.0 35.0 94-95 26.445209535674977 33.5 25.0 35.0 2.0 35.0 96-97 25.756005888564232 33.0 22.0 35.0 2.0 35.0 98-99 24.94959942119995 33.0 10.0 35.0 2.0 35.0 100-101 21.894799179847034 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10524865452371301 1101 2 0.20133045804220728 1101 3 0.3391015377893538 1101 4 0.16668010632965036 1101 5 0.13544843190027933 1101 6 0.15537912138424304 1101 7 0.2727103851041903 1101 8 0.33357444084631993 1101 9 0.2987698921502471 1101 10-11 0.18795665565470188 1101 12-13 0.2683533993474825 1101 14-15 0.3735860336873529 1101 16-17 0.49396525619285114 1101 18-19 0.44518896550106746 1101 20-21 0.5606095653277876 1101 22-23 0.5955850462422774 1101 24-25 0.5957070100665973 1101 26-27 0.5162288142999714 1101 28-29 0.5382835342172498 1101 30-31 0.5088584318879157 1101 32-33 0.4662818306066896 1101 34-35 0.5620127471746343 1101 36-37 0.43288484066209065 1101 38-39 0.5275495950054747 1101 40-41 0.26305549490076174 1101 42-43 0.1587932498580784 1101 44-45 0.2823828476713075 1101 46-47 0.22406615076301506 1101 48-49 0.3380813355635155 1101 50-51 0.3183757656377324 1101 52-53 0.49441666841193666 1101 54-55 0.5524447333375733 1101 56-57 0.35782434037341915 1101 58-59 0.36957510241145286 1101 60-61 0.5620883877691654 1101 62-63 0.6503507431061664 1101 64-65 0.37916820843622645 1101 66-67 0.5391711766986091 1101 68-69 0.5977746932035686 1101 70-71 0.37504044380798973 1101 72-73 0.37644580549153517 1101 74-75 0.31209811868944826 1101 76-77 0.30118774916171986 1101 78-79 -0.0036952351140939754 1101 80-81 0.0737975559745152 1101 82-83 0.12908683085129624 1101 84-85 0.2660053489431533 1101 86-87 0.2810947076815218 1101 88-89 0.0392625540392082 1101 90-91 -0.13606675367100252 1101 92-93 -0.15628863597344633 1101 94-95 -0.15157960190581576 1101 96-97 -0.21562163595321593 1101 98-99 -0.21031577806955326 1101 100-101 0.3678490482204815 1102 1 0.01306139307664722 1102 2 0.0959415329125477 1102 3 0.30861302765504917 1102 4 0.16325651035098332 1102 5 0.2146907993631615 1102 6 0.21463971718247166 1102 7 0.2409727250857756 1102 8 0.23211571137054676 1102 9 0.2892694133094551 1102 10-11 0.3333056542528823 1102 12-13 0.2855211139121039 1102 14-15 0.32571467761949435 1102 16-17 0.19217874053452988 1102 18-19 0.11016696658365532 1102 20-21 0.04657282181526767 1102 22-23 0.20491573322844658 1102 24-25 0.24827100586575312 1102 26-27 0.4048636322710806 1102 28-29 0.22869908474535805 1102 30-31 0.1994787042025905 1102 32-33 0.4494541623994053 1102 34-35 0.17484915017814728 1102 36-37 0.013769608898520858 1102 38-39 0.05528072243264859 1102 40-41 0.18533986618511733 1102 42-43 0.16500314405045202 1102 44-45 -0.04302995010058197 1102 46-47 0.2620877286706431 1102 48-49 0.07549730710140778 1102 50-51 -0.04712896031265501 1102 52-53 -0.1987308191775341 1102 54-55 0.01643451806741325 1102 56-57 -0.08907406672915386 1102 58-59 0.014499621373083471 1102 60-61 -0.06690253044853023 1102 62-63 0.1467444011259431 1102 64-65 8.620094142628432E-4 1102 66-67 0.0027191324879609624 1102 68-69 0.2006005478302768 1102 70-71 -0.26641190112393076 1102 72-73 -0.31237476569381784 1102 74-75 -0.1733955499712323 1102 76-77 -0.2700542759415683 1102 78-79 -0.46244899991305033 1102 80-81 -0.3611263615445779 1102 82-83 -0.284919609210327 1102 84-85 -0.3659789025153444 1102 86-87 -0.46126003237963786 1102 88-89 -0.6496667394508719 1102 90-91 -0.8420561960412378 1102 92-93 -0.7728574525658587 1102 94-95 -0.8101938783010496 1102 96-97 -0.6911602740097251 1102 98-99 -0.3549708045098896 1102 100-101 -0.21557103163258162 1103 1 0.33113213442248934 1103 2 0.4101372794948759 1103 3 0.55485323543855 1103 4 0.47914887626220093 1103 5 0.303375814653684 1103 6 0.32259497670239057 1103 7 0.3703347124344418 1103 8 0.26564314354068586 1103 9 0.4277428748318073 1103 10-11 0.4726344074122224 1103 12-13 0.47631398671452274 1103 14-15 0.5528138076657072 1103 16-17 0.6400897343484999 1103 18-19 0.5746915640729426 1103 20-21 0.5163129527970796 1103 22-23 0.5434017623013858 1103 24-25 0.6311728532956948 1103 26-27 0.7519689064531363 1103 28-29 0.6555105575226534 1103 30-31 0.6758866189666719 1103 32-33 0.7151356727397058 1103 34-35 0.6138022732144748 1103 36-37 0.6708919190935916 1103 38-39 0.6804186163904475 1103 40-41 0.776796724576748 1103 42-43 0.6699359076548248 1103 44-45 0.6348986382842057 1103 46-47 0.7983136932301491 1103 48-49 0.8261396485757189 1103 50-51 0.8209501594002404 1103 52-53 0.6736352316791638 1103 54-55 0.7680997185198422 1103 56-57 0.5506736047830003 1103 58-59 0.7008694145670518 1103 60-61 0.7078939450517296 1103 62-63 0.8237723150521816 1103 64-65 0.8766352628738332 1103 66-67 0.7092388984522344 1103 68-69 0.5453703521954054 1103 70-71 0.49081793144836894 1103 72-73 0.41766028380836673 1103 74-75 0.44037569268024157 1103 76-77 0.5039776735648083 1103 78-79 0.5467288029998301 1103 80-81 0.3107488566420642 1103 82-83 0.3951140438973475 1103 84-85 0.44228914937467323 1103 86-87 0.5718100805886763 1103 88-89 0.380697859155525 1103 90-91 0.1521766946350347 1103 92-93 0.019631341368203437 1103 94-95 0.11827581295612788 1103 96-97 0.2566529721885935 1103 98-99 0.5082279163927375 1103 100-101 0.3779105987976443 1104 1 0.1493143675068609 1104 2 0.048738070423610225 1104 3 0.24971128586539493 1104 4 0.33169064190906283 1104 5 0.11896801855173322 1104 6 0.06123935314430895 1104 7 0.20792961361399165 1104 8 0.25178477592083937 1104 9 0.21315838625417882 1104 10-11 0.27183982753321345 1104 12-13 0.26281343105627997 1104 14-15 0.4194946225094256 1104 16-17 0.25894925436018923 1104 18-19 0.4487499686606 1104 20-21 0.29997135190802027 1104 22-23 0.4507973795612443 1104 24-25 0.3971289163600531 1104 26-27 0.4246668040796706 1104 28-29 0.2751242666498186 1104 30-31 0.45135276717110173 1104 32-33 0.5390022782497397 1104 34-35 0.37679748793587464 1104 36-37 0.6741802292764021 1104 38-39 0.2983105836488207 1104 40-41 0.24012898538698124 1104 42-43 0.2922548851523743 1104 44-45 0.4942954239096622 1104 46-47 0.5566434324701177 1104 48-49 0.5926068715852253 1104 50-51 0.35280295446389687 1104 52-53 0.5043568571677852 1104 54-55 0.4377101187991528 1104 56-57 0.410218718071107 1104 58-59 0.21329242680523208 1104 60-61 0.3384131603299352 1104 62-63 0.42260417765970004 1104 64-65 0.31994214824393197 1104 66-67 0.1061100975558773 1104 68-69 0.041019787751608305 1104 70-71 0.19385796394352894 1104 72-73 0.23646549307587605 1104 74-75 0.07683942428475987 1104 76-77 0.03979424016735322 1104 78-79 0.08971872356466193 1104 80-81 0.14611313797171732 1104 82-83 0.2768832049789083 1104 84-85 0.5055440516039269 1104 86-87 0.5224740180263474 1104 88-89 0.6910849030764901 1104 90-91 0.8507189760057621 1104 92-93 0.9031279492286579 1104 94-95 1.0164226299585835 1104 96-97 0.8077267503679941 1104 98-99 0.6290773090480108 1104 100-101 1.1682003854111862 1105 1 0.027244745383619318 1105 2 0.10626988845620389 1105 3 -0.02804180839039816 1105 4 0.06339267410464089 1105 5 0.10045610662448468 1105 6 0.1332405788088451 1105 7 0.10798761381096966 1105 8 0.09530462183730037 1105 9 -0.10767025052232526 1105 10-11 0.18474541853333193 1105 12-13 0.22341816518557067 1105 14-15 0.2815920409534911 1105 16-17 0.13188833226648455 1105 18-19 0.09482510627427843 1105 20-21 -0.031920001685250554 1105 22-23 0.08493760871674283 1105 24-25 0.16825247866649562 1105 26-27 0.14281648836161054 1105 28-29 -0.052386430556317976 1105 30-31 0.04984366770624149 1105 32-33 0.24222074201055932 1105 34-35 0.17492616082570578 1105 36-37 0.2639448360241161 1105 38-39 0.21206100770687186 1105 40-41 0.04164246222520518 1105 42-43 0.20853871404085567 1105 44-45 0.22527737818799665 1105 46-47 0.3128489063754998 1105 48-49 0.3228610875429325 1105 50-51 0.2101301302920433 1105 52-53 0.025833345201142777 1105 54-55 0.05666086240545809 1105 56-57 0.04291660270543218 1105 58-59 -0.04204851586212044 1105 60-61 0.19673728294016257 1105 62-63 0.21512762396573493 1105 64-65 0.23243079443178516 1105 66-67 0.2575551779354015 1105 68-69 0.35409281328263376 1105 70-71 0.3781136463213244 1105 72-73 0.34948599012663095 1105 74-75 0.19141170019060283 1105 76-77 0.3575412061004428 1105 78-79 0.38641927839673684 1105 80-81 0.5773566403081425 1105 82-83 0.4572922705191296 1105 84-85 0.5763546317153292 1105 86-87 0.5205107660756809 1105 88-89 0.5013969003625185 1105 90-91 0.5621001467342701 1105 92-93 0.6130886623027791 1105 94-95 0.3940115727134845 1105 96-97 0.44174113003947113 1105 98-99 0.7407802167182602 1105 100-101 0.6566627235851676 1106 1 0.28207156138429923 1106 2 0.2444879260960704 1106 3 0.20203161179320261 1106 4 0.1489555087435761 1106 5 0.35754858185719485 1106 6 0.2970562909418817 1106 7 0.4253231130575301 1106 8 0.41844961334107467 1106 9 0.4202283505399649 1106 10-11 0.3641161637428567 1106 12-13 0.38004361537846876 1106 14-15 0.39790585613126694 1106 16-17 0.44964490214390196 1106 18-19 0.5118688484593577 1106 20-21 0.38281426427321463 1106 22-23 0.47854887716529504 1106 24-25 0.5522870620309916 1106 26-27 0.5885614307048641 1106 28-29 0.42905974400630953 1106 30-31 0.2924508857545689 1106 32-33 0.5468332822206605 1106 34-35 0.5808393176798603 1106 36-37 0.5499586650950974 1106 38-39 0.5624804752769492 1106 40-41 0.4593894137379664 1106 42-43 0.3531358648674967 1106 44-45 0.36354564506555676 1106 46-47 0.23388533860343586 1106 48-49 0.31194851847773464 1106 50-51 0.003145060131416244 1106 52-53 0.2025333911555478 1106 54-55 0.29509584760386787 1106 56-57 0.3463150915534783 1106 58-59 0.0776541697990325 1106 60-61 0.3023982248986812 1106 62-63 0.3441152376494685 1106 64-65 0.22053394604927234 1106 66-67 0.24958155362872247 1106 68-69 0.25862840657542563 1106 70-71 0.33022060253639296 1106 72-73 0.28833670241996145 1106 74-75 0.22274085131648746 1106 76-77 0.0042896644903294145 1106 78-79 0.29395152663604307 1106 80-81 0.221713332018183 1106 82-83 0.2102883911120479 1106 84-85 0.5171012305547116 1106 86-87 0.2144768453096333 1106 88-89 0.03775966232936767 1106 90-91 0.05234071272159113 1106 92-93 -0.11410245460310264 1106 94-95 0.17181345577554197 1106 96-97 0.33918905728188875 1106 98-99 0.4632455104524347 1106 100-101 0.855978750986818 1107 1 -0.18939022977432884 1107 2 -0.023761394129827806 1107 3 -0.030887910334826074 1107 4 0.011751871798317381 1107 5 -0.039761440248277324 1107 6 0.031911580056060984 1107 7 0.02923114772088553 1107 8 -0.05282762376167227 1107 9 -0.08952430601313921 1107 10-11 0.13341744208727846 1107 12-13 0.04114905316119888 1107 14-15 0.27330931180343754 1107 16-17 0.09070879475805782 1107 18-19 0.09035353662451229 1107 20-21 0.20607607265689154 1107 22-23 0.21360820104126077 1107 24-25 0.24313133531864395 1107 26-27 0.12750131119585006 1107 28-29 0.10657408795765377 1107 30-31 0.20368038487485762 1107 32-33 0.09098784009066208 1107 34-35 0.3349948598381616 1107 36-37 0.40184685814683974 1107 38-39 0.49059154400898564 1107 40-41 0.33133863266419183 1107 42-43 0.31600666472478167 1107 44-45 0.06478748880159202 1107 46-47 0.3146215862503752 1107 48-49 0.4038451600040389 1107 50-51 0.422842514515807 1107 52-53 0.25559678342314385 1107 54-55 0.2788725207914453 1107 56-57 -0.008843809488638499 1107 58-59 -0.032560487073965305 1107 60-61 -0.1525808384689853 1107 62-63 -0.19828810729027424 1107 64-65 -0.2776246145931367 1107 66-67 -0.33177705130666624 1107 68-69 -0.22058260432966392 1107 70-71 -0.2033866910398956 1107 72-73 -0.28553926084967785 1107 74-75 -0.20007044011077113 1107 76-77 -0.0063570539334953935 1107 78-79 -0.0239387334211294 1107 80-81 -0.03557962909012957 1107 82-83 0.051414006804886725 1107 84-85 0.055788887169335055 1107 86-87 0.10042243877395762 1107 88-89 0.1815529043699584 1107 90-91 0.5062913998778633 1107 92-93 0.4523564862206655 1107 94-95 0.7319514201853181 1107 96-97 0.3456269179312734 1107 98-99 0.28498884907631705 1107 100-101 0.19598166283970286 1108 1 0.3535167107796724 1108 2 0.3699952196267873 1108 3 0.40855186557553935 1108 4 0.23849945972237663 1108 5 0.33004248132034775 1108 6 0.4346193298226382 1108 7 0.4216183248139487 1108 8 0.40277150477863444 1108 9 0.41940193114839275 1108 10-11 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-1.0810498675900675 2316 62-63 -1.5098499961967775 2316 64-65 -1.3698898225631169 2316 66-67 -1.3915040081271286 2316 68-69 -1.3844349377158487 2316 70-71 -1.2115769954635525 2316 72-73 -1.1613105599353517 2316 74-75 -1.1148047300531658 2316 76-77 -1.5990384661210157 2316 78-79 -1.423225570797591 2316 80-81 -1.1974582956510922 2316 82-83 -0.8441847987550766 2316 84-85 -1.083656041745627 2316 86-87 -1.1281034055260264 2316 88-89 -1.1517457452785784 2316 90-91 -1.2831191756731144 2316 92-93 -1.5364043483018222 2316 94-95 -1.4140107382629665 2316 96-97 -1.2094854572074603 2316 98-99 -1.2729808010478862 2316 100-101 -1.537708747286615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3054.0 3 580.0 4 501.0 5 499.0 6 524.0 7 655.0 8 815.0 9 888.0 10 1168.0 11 1760.0 12 2665.0 13 4367.0 14 4093.0 15 4370.0 16 5882.0 17 8150.0 18 6854.0 19 4051.0 20 4005.0 21 4282.0 22 4658.0 23 5354.0 24 6149.0 25 6987.0 26 8352.0 27 9910.0 28 11489.0 29 13412.0 30 16401.0 31 19699.0 32 24173.0 33 31498.0 34 42362.0 35 66139.0 36 83013.0 37 137042.0 38 146386.0 39 23774.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.37197990479153 24.019125751959226 16.230971613976987 19.377922729272264 2 13.879690524947408 25.271140123426587 39.720298292575535 21.128871059050468 3 18.63028520464223 29.880086000341095 32.93399278111571 18.555636013900966 4 10.498532822419088 19.919644242638597 43.439081025388674 26.142741909553642 5 9.544508883880384 41.133316928308176 37.7026626474355 11.619511540375935 6 23.585381565837594 42.4409984670571 19.468794061627936 14.504825905477373 7 21.925520780202596 36.693361877785215 26.148637035028905 15.232480306983282 8 21.442395972057053 34.60489853982876 23.79524343873192 20.157462049382268 9 23.074837613335273 20.119316466734492 26.832928997059714 29.972916922870528 10-11 20.956866723682076 28.659606817254428 30.863853347419496 19.519673111644 12-13 23.784306875349355 28.402948574622695 30.00670765790945 17.806036892118502 14-15 21.07806421320932 29.93211443008541 30.5271262800161 18.462695076689172 16-17 16.441181628704644 32.67847997255471 32.139635595310544 18.7407028034301 18-19 16.243216765821025 32.97451893715472 34.07003833409498 16.712225962929267 20-21 17.568705439904182 31.07626235698668 33.09362974495361 18.261402458155523 22-23 17.33202409739597 30.329941433823016 33.43597556714144 18.902058901639574 24-25 18.53367777144071 31.216940092372035 33.10058599210476 17.148796144082485 26-27 18.01257342945705 29.94698751393456 35.307084522940045 16.733354533668347 28-29 16.50496632737784 31.334129138489665 35.03609078888147 17.12481374525103 30-31 17.155220791055843 30.838696421820046 33.968494461267305 18.037588325856806 32-33 17.13565960539326 31.799279547463566 33.80529612975133 17.25976471739184 34-35 18.45458207099452 30.21305360488941 33.75332294275257 17.5790413813635 36-37 18.220168615604837 29.813245762682133 34.357867851115856 17.60871777059717 38-39 17.39151785213204 31.15761058669031 34.17804990291044 17.272821658267205 40-41 16.738639320565056 31.198655045368373 34.73554345607611 17.327162177990466 42-43 16.603851156708092 31.44075300034097 32.917385209683594 19.038010633267337 44-45 17.115157519679876 30.636470863121822 33.083868994033764 19.16450262316454 46-47 18.36617137147981 31.40418921782277 32.651735310585025 17.5779041001124 48-49 17.702375923303528 31.00250798921579 32.91788475600086 18.377231331479827 50-51 16.394087964785456 31.226523461977568 34.124650110554185 18.254738462682788 52-53 17.13321577507405 32.28160091614045 32.56256188123154 18.02262142755396 54-55 16.70945511212584 30.790069525669207 33.13018469312651 19.370290669078443 56-57 17.927182068679116 31.057124097176626 31.959316127077127 19.056377707067135 58-59 18.6036496365724 30.119005618872002 32.65489773316285 18.62244701139275 60-61 17.575070479908405 31.39060487571628 33.03823267729702 17.996091967078293 62-63 17.482723541586275 30.89914757778791 33.181043252534465 18.437085628091346 64-65 17.33985764500231 31.483499634042534 32.75449131210663 18.42215140884852 66-67 17.360745590949602 30.579405015053585 31.72684590605723 20.33300348793959 68-69 16.831212694633642 30.55186674228344 32.6896081216906 19.927312441392324 70-71 18.566019196013407 30.448526960397253 31.95957256408907 19.025881279500272 72-73 18.151852007803836 29.043749210493075 33.12904402992407 19.67535475177902 74-75 17.094864641998704 31.651560608453078 31.94855869607172 19.3050160534765 76-77 17.744610747352734 29.96714948457948 32.40925764290184 19.878982125165937 78-79 17.716358952882224 29.796080428589566 32.81899218035893 19.668568438169277 80-81 18.649662278769444 30.042527339003644 31.529510970678444 19.778299411548467 82-83 18.195926557358646 29.3467477706917 32.70540857204998 19.75191709989967 84-85 17.869413637610602 29.42918544369208 32.19811700025048 20.503283918446844 86-87 18.108473330002976 29.800136395017933 32.08145532922838 20.009934945750707 88-89 18.725485931117134 29.074545222361465 31.54268658056893 20.657282265952475 90-91 18.770736376446283 28.040001908729067 32.1882596320644 21.001002082760248 92-93 19.609641621758563 29.16999719989586 31.826095988131485 19.394265190214092 94-95 19.267036945021104 28.09504559701419 32.003461358688554 20.634456099276147 96-97 18.75575448288252 27.706431546917283 32.260753865328255 21.277060104871943 98-99 19.429432021620695 28.25722789384188 31.132384655818424 21.180955428719002 100-101 19.674053221929242 26.886715006013183 32.16175671939837 21.277475052659202 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11715.0 1 6857.5 2 2030.5 3 1866.5 4 1425.5 5 1399.0 6 2229.0 7 3685.0 8 5034.0 9 5114.5 10 3893.0 11 2865.5 12 2574.0 13 2419.0 14 2276.5 15 2178.0 16 2117.0 17 2029.5 18 1859.5 19 1859.5 20 2053.0 21 2315.5 22 2636.0 23 2950.5 24 3208.5 25 3752.0 26 5319.5 27 7067.5 28 7387.5 29 8026.5 30 10771.5 31 13353.0 32 17232.0 33 23416.5 34 28347.5 35 31414.0 36 36118.0 37 39982.5 38 38401.0 39 35533.5 40 32567.0 41 29218.0 42 25608.5 43 23970.0 44 23637.5 45 22315.0 46 22165.5 47 25798.5 48 38699.5 49 37196.0 50 20116.5 51 10433.5 52 5892.5 53 4229.0 54 3156.5 55 2349.5 56 1757.0 57 1412.0 58 1198.0 59 1005.5 60 809.5 61 700.0 62 582.5 63 452.0 64 355.0 65 268.5 66 211.5 67 179.0 68 144.0 69 106.0 70 96.5 71 92.0 72 84.0 73 77.5 74 61.5 75 48.0 76 32.5 77 24.5 78 17.5 79 10.0 80 8.0 81 4.0 82 4.0 83 4.0 84 3.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30197049013637556 2 0.07807655133498333 3 0.08631718913241448 4 0.0900883284634423 5 0.1027984647272768 6 0.04846612399506121 7 0.04958349861166203 8 0.050700873228262874 9 0.054192668905140476 10-11 0.0513992323636384 12-13 0.05084054505533797 14-15 0.048605795822136304 16-17 0.050072350006424904 18-19 0.05768446458201809 20-21 0.059709706074607106 22-23 0.07458475565810571 24-25 0.08324440893676219 26-27 0.0791240900380466 28-29 0.07626081758300697 30-31 0.07018509310523993 32-33 0.061665111653658566 34-35 0.3039957316289646 36-37 0.47439536066059185 38-39 0.449743283181836 40-41 0.4298400478236336 42-43 0.46021867021246876 44-45 0.4707638931566392 46-47 0.4644088250247219 48-49 0.480610756965434 50-51 0.4773284690291691 52-53 0.4762110944125682 54-55 0.46783078478806195 56-57 0.4469498466403338 58-59 0.43277315619221074 60-61 0.3935253727841065 62-63 0.45938063925001815 64-65 0.4817979674955724 66-67 0.4901084412065411 68-69 0.5028185774703756 70-71 0.49953628953411067 72-73 0.48801336380041455 74-75 0.4890609025034779 76-77 0.46678324608499866 78-79 0.44799738534339717 80-81 0.4533747506857887 82-83 0.4607773575207692 84-85 0.4643389891111844 86-87 0.4648278405059473 88-89 0.46971635445357585 90-91 0.4815884597549598 92-93 0.4875245124056517 94-95 0.492622534093893 96-97 0.48549927091306266 98-99 0.48864188702225253 100-101 0.46971635445357585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 715964.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.389780122111226 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78336808539434 14.306432838445948 2 9.86668218612076 3.826255962472665 3 3.9479527486639667 2.296498071872368 4 2.141900175586987 1.6612389379257237 5 1.3774618185370644 1.3354340894018575 6 0.9966738002279515 1.159517150393339 7 0.7371137123001781 1.000473096454552 8 0.591855150634441 0.9180752987952663 9 0.4648965272701461 0.8112817298971096 >10 4.507277172588778 18.822044375914064 >50 0.8583533331543414 11.803035777439778 >100 0.6724128126221666 24.466743060192552 >500 0.030990086755362446 3.89153760025269 >1k 0.018738191991614504 7.24241307125453 >5k 0.004324198151911039 6.459018939287546 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9542 1.3327485739506457 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9415 1.3150102519121072 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9069 1.2666837997441212 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7255 1.0133191054298818 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5570 0.7779720768083311 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5370 0.7500377113933102 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4266 0.5958400143023951 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3697 0.5163667446966608 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3206 0.4477878776027845 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2792 0.3899637411936913 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2672 0.3732031219446788 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2473 0.345408428356733 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2460 0.34359269460475667 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2420 0.33800582152175246 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2358 0.32934616824309604 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2205 0.30797637870060507 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2128 0.297221648015822 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2049 0.2861875736768888 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.23911816795257865 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1686 0.2354867004486259 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1664 0.23241392025297364 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1598 0.22319557966601675 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1474 0.20587627310870377 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1458 0.2036415238755021 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1406 0.1963785888675967 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1370 0.19135040309289295 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1350 0.18855696655139084 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1119 0.15629277449704176 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1115 0.15573408718874132 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1084 0.15140426054941308 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1061 0.1481918085266857 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.14023051438340475 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 975 0.13618003139822674 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 936 0.13073283014229767 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 935 0.13059315831522256 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 917 0.12807906542787068 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 844 0.11788302205138806 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 825 0.11522925733696107 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 789 0.11020107156225732 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 745 0.10405551117095274 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 733 0.10237944924605148 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 720 0.10056371549407511 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.586873083004173E-4 0.0 18-19 0.0 0.0 0.0 0.0011173746166008346 0.0 20-21 0.0 0.0 0.0 0.0019554055790514608 0.0 22-23 0.0 0.0 0.0 0.0048186780340911 0.0 24-25 0.0 0.0 0.0 0.0076819504891307375 0.0 26-27 0.0 0.0 0.0 0.01319898765859736 0.0 28-29 0.0 0.0 0.0 0.03268320753557441 0.0 30-31 0.0 0.0 0.0 0.08436178355336302 0.0 32-33 0.0 0.0 0.0 0.18736975602125244 0.0 34-35 0.0 0.0 0.0 0.3391231961383533 0.0 36-37 0.0 0.0 0.0 0.5581984569056545 0.0 38-39 0.0 0.0 0.0 0.8456430770262192 0.0 40-41 0.0 0.0 0.0 1.1483119262979704 0.0 42-43 0.0 0.0 0.0 1.4486761904229821 0.0 44-45 0.0 0.0 0.0 1.8086803247090635 0.0 46-47 0.0 0.0 0.0 2.230489242475879 0.0 48-49 0.0 0.0 0.0 2.636026392388444 0.0 50-51 0.0 0.0 0.0 3.066634635260991 0.0 52-53 0.0 0.0 0.0 3.464280326943813 0.0 54-55 0.0 0.0 0.0 3.9000564274181384 0.0 56-57 0.0 0.0 0.0 4.477040745065394 0.0 58-59 0.0 0.0 0.0 5.0537457190585 0.0 60-61 0.0 0.0 0.0 5.67912632478728 0.0 62-63 0.0 0.0 0.0 6.2759440418792005 0.0 64-65 0.0 0.0 0.0 6.882119771385153 0.0 66-67 0.0 0.0 0.0 7.591443145186071 0.0 68-69 0.0 0.0 0.0 8.367040800934125 0.0 70-71 0.0 0.0 0.0 9.221064187584851 0.0 72-73 0.0 0.0 0.0 10.025154896056227 0.0 74-75 0.0 0.0 0.0 10.939795855657547 0.0 76-77 0.0 0.0 0.0 11.829714901866573 0.0 78-79 0.0 0.0 0.0 12.915733193289046 0.0 80-81 0.0 0.0 0.0 14.05343006072931 0.0 82-83 0.0 0.0 0.0 15.098035655424017 0.0 84-85 0.0 0.0 0.0 16.216457810727913 0.0 86-87 0.0 0.0 0.0 17.363861870149897 0.0 88-89 0.0 0.0 0.0 18.53005179031348 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 105 4.6826244E-8 36.164696 1 ATGTTGC 95 8.916395E-7 34.887077 7 CTTGCAG 55 0.0026156832 34.446033 3 GGTACTC 180 0.0 34.20683 3 TACTCCC 180 0.0 34.204433 5 GTAAGCG 35 2.0372831E-4 33.974617 94-95 AGGCACT 85 1.6678247E-5 33.421238 8 GGCATTC 190 0.0 32.395145 7 CGCCGTC 30 0.0039251214 31.709644 94-95 CATTCCG 180 7.2759576E-12 31.5645 9 CGCAGAA 135 1.2341843E-8 31.564497 9 GAGGCAC 95 3.5668305E-5 29.90321 7 TAGGCAT 175 1.5643309E-10 29.769135 5 ATGGATT 65 0.005925476 29.146646 3 GTGGTAC 215 1.8189894E-12 28.70047 1 TGGTACT 215 1.8189894E-12 28.638273 2 CATATAG 270 0.0 28.067139 3 TATAGGT 360 0.0 27.628592 3 CCTTTAG 190 3.9835868E-10 27.48041 1 CTTTAGG 190 4.0927262E-10 27.420856 2 >>END_MODULE