##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939037_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715964 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.919164930080285 31.0 31.0 34.0 30.0 34.0 2 32.175399880440914 33.0 31.0 34.0 30.0 34.0 3 32.242123905671235 34.0 31.0 34.0 30.0 34.0 4 35.85154421172014 37.0 35.0 37.0 35.0 37.0 5 35.777990234145854 37.0 35.0 37.0 35.0 37.0 6 35.84052829471873 37.0 35.0 37.0 35.0 37.0 7 35.78338296338922 37.0 35.0 37.0 33.0 37.0 8 35.77719410473152 37.0 35.0 37.0 33.0 37.0 9 37.37849668419083 39.0 37.0 39.0 34.0 39.0 10-11 37.29135543127867 39.0 37.0 39.0 33.5 39.0 12-13 37.234138867317355 39.0 37.0 39.0 33.0 39.0 14-15 38.38159460531536 40.0 38.0 41.0 33.0 41.0 16-17 38.44721312803437 40.0 38.0 41.0 34.0 41.0 18-19 38.47853313853769 40.0 38.0 41.0 34.0 41.0 20-21 38.453261616505856 40.0 38.0 41.0 34.0 41.0 22-23 38.35117198630098 40.0 38.0 41.0 34.0 41.0 24-25 38.23182380678358 40.0 37.0 41.0 33.0 41.0 26-27 38.0030895408149 40.0 37.0 41.0 33.0 41.0 28-29 37.83967140805963 40.0 37.0 41.0 33.0 41.0 30-31 37.616402500684394 40.0 36.0 41.0 33.0 41.0 32-33 37.37855394963992 39.5 36.0 41.0 32.0 41.0 34-35 37.2019654619506 39.0 35.0 41.0 32.0 41.0 36-37 37.01535342559123 39.0 35.0 41.0 31.0 41.0 38-39 36.8191103183959 39.0 35.0 41.0 31.0 41.0 40-41 36.68064455754758 39.0 35.0 41.0 31.0 41.0 42-43 36.452167986099866 39.0 35.0 41.0 30.0 41.0 44-45 36.163990787246284 39.0 35.0 41.0 30.0 41.0 46-47 35.79990195037739 39.0 35.0 40.0 28.5 41.0 48-49 35.42096110977647 39.0 35.0 40.0 27.0 41.0 50-51 35.18484169595119 38.0 35.0 40.0 26.5 41.0 52-53 34.987464453520005 38.0 34.5 40.0 26.0 41.0 54-55 34.72403640406501 38.0 34.0 40.0 25.0 41.0 56-57 34.621467001134135 38.0 34.0 40.0 24.5 41.0 58-59 34.39482990764898 38.0 34.0 40.0 24.0 41.0 60-61 33.9948356341939 38.0 33.5 40.0 23.0 41.0 62-63 33.59243202172176 37.0 33.0 40.0 22.0 41.0 64-65 33.17033537999117 37.0 32.5 39.0 21.0 40.5 66-67 32.67845939181299 36.0 32.0 39.0 19.0 40.0 68-69 32.13095490834735 35.5 31.0 38.5 16.0 40.0 70-71 31.547983557832517 35.0 31.0 38.0 12.0 40.0 72-73 30.971826097401546 35.0 30.5 37.0 7.5 39.0 74-75 30.384865021146314 34.5 29.5 37.0 4.5 39.0 76-77 28.429425920856353 32.5 27.0 35.0 2.0 37.0 78-79 29.380024693979024 34.0 29.0 36.0 2.0 38.0 80-81 29.296899704454415 34.0 29.0 35.5 2.0 37.0 82-83 28.78395841131677 34.0 29.0 35.0 2.0 37.0 84-85 28.242853690967703 33.5 27.5 35.0 2.0 36.0 86-87 27.500441362973557 33.0 26.0 35.0 2.0 36.0 88-89 26.66625835935885 32.5 25.0 35.0 2.0 35.0 90-91 25.972981881770593 32.0 23.5 34.0 2.0 35.0 92-93 25.48216390768251 32.0 22.0 34.0 2.0 35.0 94-95 24.805756993368384 31.0 19.0 34.0 2.0 35.0 96-97 23.76226388477633 31.0 4.5 34.0 2.0 35.0 98-99 22.412739327675695 31.0 2.0 34.0 2.0 35.0 100-101 17.017548368353715 22.5 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18248597008241774 1101 2 0.31593000006397887 1101 3 0.18510524249351334 1101 4 0.07150239950233583 1101 5 0.19964151591481993 1101 6 0.1000814321657657 1101 7 0.19057624982584542 1101 8 0.17160054189390195 1101 9 0.24046947097637883 1101 10-11 0.3375486881388028 1101 12-13 0.24485022513334798 1101 14-15 0.22802303695634407 1101 16-17 0.3582608834693417 1101 18-19 0.3020411365435436 1101 20-21 0.33404861146005516 1101 22-23 0.2075087196855918 1101 24-25 0.26590117420827397 1101 26-27 0.36876369073956994 1101 28-29 0.09333880838445907 1101 30-31 0.16923140368173506 1101 32-33 0.36101987308305894 1101 34-35 0.3636986924919512 1101 36-37 0.46516569269646624 1101 38-39 0.16019149771386765 1101 40-41 0.3036418099878304 1101 42-43 0.2551870304974031 1101 44-45 0.5142902396746152 1101 46-47 0.692748249184227 1101 48-49 0.12197337978177103 1101 50-51 -0.02942665154003521 1101 52-53 0.15147361099782586 1101 54-55 -0.14265504279156005 1101 56-57 0.2651924142576405 1101 58-59 0.16760721744556406 1101 60-61 0.17463046287274864 1101 62-63 0.21846584864852758 1101 64-65 0.32012973936274847 1101 66-67 0.038691206987209625 1101 68-69 0.3176948863296829 1101 70-71 0.4618153820868969 1101 72-73 0.4913809798389899 1101 74-75 0.4436141464534451 1101 76-77 0.1632325768026952 1101 78-79 0.43267863662046935 1101 80-81 0.5529061413071581 1101 82-83 0.09578106149638899 1101 84-85 0.21967490266973044 1101 86-87 0.7587187083280149 1101 88-89 0.5550782325892669 1101 90-91 0.8701899100841821 1101 92-93 0.6874818937642964 1101 94-95 0.347463741991195 1101 96-97 0.7303820857379044 1101 98-99 0.9217249510773478 1101 100-101 1.3517306051962308 1102 1 0.07526727169660319 1102 2 0.06144809654461625 1102 3 0.10427136554866223 1102 4 0.06320943221618336 1102 5 0.09867550667111402 1102 6 0.16389679855993222 1102 7 0.13243988892331515 1102 8 0.09485647419923282 1102 9 0.2137931812291285 1102 10-11 0.18400966145788544 1102 12-13 0.145486134953984 1102 14-15 0.15458112011271652 1102 16-17 0.02074752450530326 1102 18-19 0.1976353386374896 1102 20-21 0.21285562705592298 1102 22-23 0.39580534189177285 1102 24-25 0.3778458229889594 1102 26-27 0.39817076092791837 1102 28-29 0.3812574470886787 1102 30-31 0.30032390091741235 1102 32-33 0.18189640618322045 1102 34-35 0.2667542519029098 1102 36-37 0.37260946938548045 1102 38-39 0.4018950605669218 1102 40-41 0.2900908469857981 1102 42-43 0.21490772983627693 1102 44-45 0.12370420331972554 1102 46-47 0.08064630004775353 1102 48-49 0.12947753204851153 1102 50-51 -0.14300791799295354 1102 52-53 -0.3331375564021428 1102 54-55 -0.2170800868587861 1102 56-57 -0.1975771360946652 1102 58-59 -0.4562413670825549 1102 60-61 -0.3078849086105393 1102 62-63 -0.22756162339595676 1102 64-65 -0.34726906793043355 1102 66-67 -0.37327726817090223 1102 68-69 -0.4823312532882582 1102 70-71 -0.3269892277388209 1102 72-73 -0.21821249127095044 1102 74-75 -0.2312205725109635 1102 76-77 -0.6550762593134642 1102 78-79 -0.4114239879822321 1102 80-81 -0.39534551150319786 1102 82-83 -0.2901472650591508 1102 84-85 0.1848305606247358 1102 86-87 0.4961046077390634 1102 88-89 0.47344628766289887 1102 90-91 0.5687920188342481 1102 92-93 0.6070262316454631 1102 94-95 0.9236873305674855 1102 96-97 0.7711529500108298 1102 98-99 0.5966024649914878 1102 100-101 0.5386867045175237 1103 1 0.10105867828478665 1103 2 0.179003568316503 1103 3 0.1376033024874843 1103 4 0.0909208812547675 1103 5 0.2848498046337724 1103 6 0.2414036758028928 1103 7 0.31841170968211685 1103 8 0.2581927399610393 1103 9 0.30226873734324045 1103 10-11 0.269238600337701 1103 12-13 0.2768275115196843 1103 14-15 0.48000736953670753 1103 16-17 0.37621371081225874 1103 18-19 0.29255525541237404 1103 20-21 0.4014456875198036 1103 22-23 0.48128214079700626 1103 24-25 0.3421888891920233 1103 26-27 0.5043211805444656 1103 28-29 0.40975436803878296 1103 30-31 0.44058125642549584 1103 32-33 0.36567588756229696 1103 34-35 0.5088157491171046 1103 36-37 0.6150619570623022 1103 38-39 0.552189001611147 1103 40-41 0.4658620058536016 1103 42-43 0.5260348994842516 1103 44-45 0.531625949371417 1103 46-47 0.543746575137483 1103 48-49 0.6748394076856243 1103 50-51 0.6649354721736316 1103 52-53 0.620435817147353 1103 54-55 0.7058563911549811 1103 56-57 0.6451434593279544 1103 58-59 0.6969640565137212 1103 60-61 0.6789226542621236 1103 62-63 0.8072225638326174 1103 64-65 0.7136032597195694 1103 66-67 0.947973761670049 1103 68-69 1.0899565994759044 1103 70-71 0.9261526599072738 1103 72-73 0.8695509036433826 1103 74-75 0.8119838736091545 1103 76-77 0.6137227017690172 1103 78-79 0.6631260238871235 1103 80-81 0.3780580604739576 1103 82-83 0.5840201347146348 1103 84-85 0.8834816435286754 1103 86-87 0.6997931838355846 1103 88-89 0.8491532123832712 1103 90-91 0.8734608638763497 1103 92-93 0.7807691274883908 1103 94-95 0.9688816388634685 1103 96-97 0.6201747756570093 1103 98-99 1.1236122257891061 1103 100-101 1.6425714740426116 1104 1 0.08990215608926633 1104 2 0.12843524517213467 1104 3 0.14509499043346352 1104 4 0.16050252878716265 1104 5 0.06774830971912849 1104 6 0.05694339452800534 1104 7 0.10514619632007083 1104 8 0.11637323875273609 1104 9 0.11017985027991983 1104 10-11 0.13825272341314587 1104 12-13 -0.015290128290544658 1104 14-15 0.11358441662959251 1104 16-17 0.08323462957661576 1104 18-19 0.24746592792999422 1104 20-21 0.298186814673123 1104 22-23 0.3723457414167868 1104 24-25 0.30161100461833 1104 26-27 0.108880772894409 1104 28-29 0.47815509918006427 1104 30-31 0.3507677157072635 1104 32-33 0.12475525198966864 1104 34-35 0.40116067254579946 1104 36-37 0.24481671438683605 1104 38-39 0.2515505080725333 1104 40-41 0.5268531997543775 1104 42-43 0.4427124054801084 1104 44-45 0.647222260804746 1104 46-47 0.6242942008679861 1104 48-49 0.49851090527016595 1104 50-51 0.5342257128980208 1104 52-53 0.5609005163046206 1104 54-55 0.6029101473818841 1104 56-57 0.6423412074858348 1104 58-59 0.7719083195420211 1104 60-61 0.8684386268694269 1104 62-63 0.5783313017011267 1104 64-65 0.597463840844533 1104 66-67 0.6693539391612191 1104 68-69 0.4049303911188957 1104 70-71 0.42042949409292163 1104 72-73 0.4007715946677308 1104 74-75 0.6493437324925004 1104 76-77 0.09470481345290338 1104 78-79 0.3972874819708636 1104 80-81 0.5732401497945929 1104 82-83 0.5805492674085571 1104 84-85 0.7471388560183208 1104 86-87 0.8870112186537895 1104 88-89 0.8754517069715924 1104 90-91 1.13409766876158 1104 92-93 1.1204885371899351 1104 94-95 1.0863582886664211 1104 96-97 1.371100682566567 1104 98-99 1.892549674872786 1104 100-101 2.273958976638344 1105 1 -0.10512514777704851 1105 2 0.0031767065582357645 1105 3 -0.07146023560315484 1105 4 -0.10301972468464271 1105 5 -0.05713377478605963 1105 6 -0.06063723121751963 1105 7 -0.02975569579955817 1105 8 -0.021605946836949386 1105 9 -0.14582645313771536 1105 10-11 -0.08248289417952037 1105 12-13 0.05498969530130182 1105 14-15 0.07499787048661233 1105 16-17 -0.20198735796974887 1105 18-19 -0.120012376713305 1105 20-21 -0.06475324814218197 1105 22-23 0.03274255031161033 1105 24-25 -0.18114433304130984 1105 26-27 -0.39089707540771457 1105 28-29 -0.22828182834157928 1105 30-31 -0.39815709196189175 1105 32-33 -0.34095678250402983 1105 34-35 -0.18601143483116545 1105 36-37 -0.1266460943547827 1105 38-39 -0.11755180208626115 1105 40-41 -0.12259470403096628 1105 42-43 -0.09033457467944572 1105 44-45 -0.15408547948569407 1105 46-47 -0.40379978133798744 1105 48-49 -0.5421111194838844 1105 50-51 -0.5012156904878751 1105 52-53 -0.4502037855590473 1105 54-55 -0.8139427400430179 1105 56-57 -0.5439565195630465 1105 58-59 -0.49987403745465997 1105 60-61 -0.42963204582342485 1105 62-63 -0.5215034524546454 1105 64-65 -0.47413685515783044 1105 66-67 -0.41808518799053473 1105 68-69 -0.13669295779059354 1105 70-71 -0.32492556560350394 1105 72-73 -0.3199268152513959 1105 74-75 -0.4412230279229128 1105 76-77 -0.6963762883319724 1105 78-79 -0.5190346489344861 1105 80-81 -0.5467272387738973 1105 82-83 -0.40661080219167545 1105 84-85 -0.22518082355794888 1105 86-87 -0.38174199597976965 1105 88-89 -0.39126274602088884 1105 90-91 -0.2968087758263458 1105 92-93 -0.23549369067423243 1105 94-95 -0.2863017316883649 1105 96-97 -0.14124241595716214 1105 98-99 0.29130212346600715 1105 100-101 0.2393295760101921 1106 1 0.0481338747833604 1106 2 0.07110373523164526 1106 3 -0.04893830038817981 1106 4 -0.02025262804144745 1106 5 0.04312592105987534 1106 6 0.08812253409438853 1106 7 0.1760324223139591 1106 8 0.1959037516807527 1106 9 0.15655783259184375 1106 10-11 0.22347621708358645 1106 12-13 0.07859850572334182 1106 14-15 0.16719523932951574 1106 16-17 0.26510928522098 1106 18-19 0.2691381079454018 1106 20-21 0.12462059631817368 1106 22-23 -0.06413148117250955 1106 24-25 0.20782554010315124 1106 26-27 0.2998222713984475 1106 28-29 0.27508459929091345 1106 30-31 0.005215747913716484 1106 32-33 0.1365330380600298 1106 34-35 0.20647982848760194 1106 36-37 0.47336459271706843 1106 38-39 0.41607293207282936 1106 40-41 0.2656730284930049 1106 42-43 0.044778868588231546 1106 44-45 0.3189863055759119 1106 46-47 0.017664133763908296 1106 48-49 0.028589204334394935 1106 50-51 -0.3278564231935235 1106 52-53 -0.4061324386673846 1106 54-55 -0.4356214652890813 1106 56-57 -0.2076525364913664 1106 58-59 -0.1429066009625899 1106 60-61 -0.37077192890686206 1106 62-63 -0.2891804036467889 1106 64-65 -0.10163682844015653 1106 66-67 -0.3170079311481402 1106 68-69 -0.4594906194592703 1106 70-71 -0.2115122217255916 1106 72-73 -0.17518859507634232 1106 74-75 -0.4134195418000779 1106 76-77 -0.5169959247577864 1106 78-79 -0.15915391665632228 1106 80-81 -0.24787930511923761 1106 82-83 -0.41264044812898604 1106 84-85 -0.10121185881460093 1106 86-87 -0.07667311780170039 1106 88-89 0.22690848224427995 1106 90-91 -0.15699836448095184 1106 92-93 -0.01507069637044367 1106 94-95 0.1282198615403054 1106 96-97 0.2911201750165411 1106 98-99 0.1918279274009862 1106 100-101 0.20941218707558562 1107 1 -0.006106562240645985 1107 2 0.09321126052580553 1107 3 -0.04981595745393719 1107 4 0.032576995755363214 1107 5 -0.0591252283017738 1107 6 0.05667042177068282 1107 7 0.06755154826594634 1107 8 0.016759180235773385 1107 9 0.06052820551406768 1107 10-11 0.07183635176703262 1107 12-13 0.1004240734321229 1107 14-15 0.1659269428454806 1107 16-17 0.0015958271291012238 1107 18-19 -0.03661157113560165 1107 20-21 0.019681109542496245 1107 22-23 0.12222710325687558 1107 24-25 0.09978964638987975 1107 26-27 -0.018052609154061372 1107 28-29 0.08216716877551278 1107 30-31 0.06710492525375145 1107 32-33 0.2454291587337778 1107 34-35 0.19138740791760966 1107 36-37 0.0754465675274858 1107 38-39 0.024251342529254316 1107 40-41 -0.02204776992885371 1107 42-43 0.06464303680309769 1107 44-45 -0.012021646653295193 1107 46-47 0.20548889763725242 1107 48-49 0.17017087360532202 1107 50-51 -0.19010014938092468 1107 52-53 -0.11751552777418084 1107 54-55 -0.30617627579307793 1107 56-57 -0.316136486440314 1107 58-59 -0.10733483199145155 1107 60-61 -0.07728074033772714 1107 62-63 0.01972720039043452 1107 64-65 -0.3341564834471029 1107 66-67 -0.32532414196629134 1107 68-69 -0.46011502734834053 1107 70-71 -0.23205308814537062 1107 72-73 -0.3796816660968432 1107 74-75 -0.27224799265333033 1107 76-77 -0.3448264713248754 1107 78-79 0.1041042416582485 1107 80-81 0.10608717166180881 1107 82-83 -0.14720660255562024 1107 84-85 -0.03117051870608023 1107 86-87 -0.3316714568151973 1107 88-89 -0.03595288485014336 1107 90-91 0.2303447133065326 1107 92-93 0.2771686005196159 1107 94-95 0.13636689780722477 1107 96-97 0.30567226232291134 1107 98-99 0.8121602477376122 1107 100-101 0.550655712304124 1108 1 0.17247420339894148 1108 2 0.1631334516244678 1108 3 0.16989148107703045 1108 4 0.0831278827768358 1108 5 0.05158750123448641 1108 6 0.11864365550862743 1108 7 0.16841678324882992 1108 8 0.22105423158965465 1108 9 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-0.9130839507667652 2316 70-71 -0.8161957074572435 2316 72-73 -0.7497978729569468 2316 74-75 -1.0894154256642743 2316 76-77 -0.6646761092932714 2316 78-79 -0.7479037785309615 2316 80-81 -0.9411503554244938 2316 82-83 -0.884632941015532 2316 84-85 -1.4662426607704546 2316 86-87 -1.5562780447333857 2316 88-89 -1.6759824815944988 2316 90-91 -1.8421821748072027 2316 92-93 -2.2351820944753626 2316 94-95 -1.7970767626758324 2316 96-97 -1.5642936485697483 2316 98-99 -1.3402646373107459 2316 100-101 -0.6063821028219252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 25.0 5 87.0 6 213.0 7 313.0 8 400.0 9 707.0 10 1204.0 11 1736.0 12 2297.0 13 3131.0 14 3641.0 15 5101.0 16 6167.0 17 8595.0 18 7961.0 19 5663.0 20 5411.0 21 5837.0 22 6581.0 23 7372.0 24 8502.0 25 9773.0 26 11158.0 27 13154.0 28 15572.0 29 18344.0 30 21927.0 31 25906.0 32 31721.0 33 39240.0 34 53641.0 35 73302.0 36 98938.0 37 140797.0 38 78629.0 39 2910.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.67162747923805 25.1204221363644 16.81087416097033 19.397076223427224 2 13.947628651719917 26.1035471057204 39.02654323401735 20.922281008542328 3 18.424110709477013 30.179729707080245 33.14258817482443 18.25357140861831 4 10.674559056041923 20.904123671022564 42.556469319686464 25.864847953249047 5 9.240688079288903 41.65251325485639 37.665999966478765 11.440798699375947 6 23.365141264085903 43.01473258431988 19.107524959355498 14.512601192238716 7 21.756959847143154 37.06359537630384 25.687464732863663 15.491980043689345 8 21.556251431636227 35.223279382762264 23.38092418054539 19.83954500505612 9 22.569849880720263 20.257862127146055 26.469347620830096 30.702940371303583 10-11 20.92647116335458 29.059072802543145 30.591971104692416 19.42248492940986 12-13 23.634149203032553 28.51798414445419 29.908556854813927 17.939309797699327 14-15 20.7805367927638 30.20283369369067 30.24103436524032 18.7755951483052 16-17 16.473892766404404 32.97788535807618 31.809928333084247 18.738293542435166 18-19 16.122118468826145 33.180601443794735 33.75395709679515 16.94332299058398 20-21 17.521132347436463 31.184109815577322 32.87127565073104 18.423482186255175 22-23 17.287391544826274 30.69309350749479 33.17827432664212 18.84124062103681 24-25 18.4393349386282 31.604382343246307 32.89858149292422 17.057701225201267 26-27 17.975833980479468 30.16359761105307 35.05986334508439 16.800705063383077 28-29 16.375613857679998 31.652778631327834 34.76236235341442 17.209245157577755 30-31 17.106516528764015 31.213021883781867 33.60881273360113 18.071648853852988 32-33 17.189272086306016 31.89929940611539 33.596451776904985 17.31497673067361 34-35 18.359651371264178 30.362813866087063 33.688112916710026 17.58942184593873 36-37 18.095342539240594 30.104979194854494 34.16360660707438 17.63607165883054 38-39 17.226420932134552 31.26787536431695 34.15566120426962 17.350042499278874 40-41 16.708791627061753 31.28628607306207 34.52831335104002 17.476608948836155 42-43 16.59955135403921 31.608169490957245 32.85256141644737 18.939717738556176 44-45 17.170793776721226 30.85946502373746 32.86719733168333 19.102543867857985 46-47 18.29456595598767 31.63535316641956 32.378133648807854 17.69194722878491 48-49 17.70465814651861 31.10440673231371 32.745373280257 18.445561840910678 50-51 16.299561009608066 31.41588717821222 33.76468830006104 18.519863512118675 52-53 16.99855020643496 32.47383946678883 32.33800861495829 18.18960171181791 54-55 16.58910224536429 30.980188948047665 32.75269426954428 19.67801453704376 56-57 17.88127615354962 31.24263231112179 31.630780318563502 19.24531121676509 58-59 18.378597701968193 30.488972506164796 32.368126453903535 18.76430333796348 60-61 17.30920320720049 31.953840448689615 32.42181907573348 18.315137268376418 62-63 17.184970605776602 31.350067112969075 32.78674557270897 18.678216708545346 64-65 16.969453600759817 31.918682607966925 32.299499972065476 18.81236381920778 66-67 17.093863093217795 31.051879538569192 31.19399899713247 20.66025837108054 68-69 16.55981562958307 31.10775892171241 31.971157203715343 20.361268244989176 70-71 18.009507467908627 31.086431082031417 31.478071158655435 19.42599029140452 72-73 17.681615276745756 29.73403690688359 32.39296947891235 20.191378337458307 74-75 16.546851517674074 32.304347704633194 31.035359319742334 20.113441457950398 76-77 17.214397496087635 30.483721144793428 31.70017075306786 20.601710606051075 78-79 16.9452984122949 30.464955813274596 32.141914325103116 20.447831449327385 80-81 17.691614167482 30.751358134876988 30.98377221743846 20.57325548020255 82-83 17.230754618947323 30.175679224095063 31.908936762183576 20.68462939477404 84-85 16.68613928802137 30.12336539972415 31.519248563996022 21.67124674825846 86-87 16.85537369443371 30.681043768054963 31.246920216033956 21.216662321477372 88-89 17.35651303139231 29.877182475957525 30.786848412853136 21.97945607979703 90-91 17.110165951254796 28.995699117674274 31.561088510139594 22.333046420931336 92-93 17.720932896067403 30.37527026498539 31.114204066126227 20.78959277282098 94-95 17.194432358858787 29.108670404779048 31.423888395765953 22.27300884059621 96-97 16.549784626042648 29.158239799766466 31.494879630819426 22.797095943371453 98-99 17.076556921856405 29.468311255873203 30.461587454117804 22.993544368152588 100-101 16.69121421313416 28.455320322784523 31.840577129229043 23.012888334852278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10732.0 1 6520.5 2 2109.5 3 1733.5 4 1377.5 5 1367.5 6 2138.5 7 3546.5 8 4621.0 9 4604.5 10 3652.5 11 2658.0 12 2353.5 13 2283.5 14 2128.0 15 1973.0 16 1867.0 17 1817.0 18 1812.0 19 1913.0 20 2072.0 21 2357.0 22 2687.0 23 2984.5 24 3161.5 25 3739.5 26 5440.0 27 7138.5 28 7538.0 29 8398.5 30 11054.0 31 13429.0 32 17479.5 33 23772.5 34 28450.0 35 31579.0 36 36363.0 37 39949.5 38 38508.0 39 35927.5 40 33171.0 41 29675.5 42 26065.5 43 24497.5 44 24085.5 45 23019.0 46 22343.0 47 26391.5 48 39277.5 49 37880.0 50 21919.5 51 11418.5 52 5290.5 53 2806.5 54 1840.5 55 1415.0 56 1163.0 57 1055.5 58 968.5 59 801.5 60 681.5 61 600.5 62 472.5 63 312.5 64 219.5 65 176.5 66 114.0 67 88.5 68 71.0 69 57.5 70 48.5 71 39.5 72 30.0 73 24.0 74 20.0 75 14.5 76 10.5 77 8.5 78 7.5 79 4.0 80 2.5 81 2.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36873362347827543 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011173746166008346 16-17 0.0018157337519763565 18-19 4.888513947628652E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01026587929002017 36-37 0.021718969110178725 38-39 0.009707191981719751 40-41 0.005377365342391517 42-43 0.004469498466403338 44-45 9.777027895257304E-4 46-47 7.681950489130737E-4 48-49 0.0019554055790514608 50-51 4.19015481225313E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.888513947628652E-4 60-61 0.001745897838438804 62-63 9.777027895257304E-4 64-65 5.586873083004173E-4 66-67 0.002374421060276774 68-69 0.0019554055790514608 70-71 6.983591353755216E-5 72-73 0.0 74-75 0.0 76-77 0.08429194763982546 78-79 0.003491795676877608 80-81 6.983591353755216E-5 82-83 0.0 84-85 2.095077406126565E-4 86-87 0.08568866591057651 88-89 0.41273024900693334 90-91 0.36621953059092355 92-93 0.0 94-95 7.681950489130737E-4 96-97 0.0 98-99 0.0 100-101 9.078668759881783E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 715964.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.663214247674333 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37948329817613 13.50833803929827 2 10.528329263174836 3.929849294173824 3 4.108834501996969 2.300521758570171 4 2.2486982547654253 1.6787174922823394 5 1.5187750062382666 1.4172611667718844 6 1.014368705776921 1.135882828923048 7 0.7769643233594123 1.015045614075922 8 0.600891774472249 0.897165754131263 9 0.48261397701631437 0.8106415246779886 >10 4.661322020813094 18.827402103224454 >50 0.9165503879321703 12.065925836469784 >100 0.7033121344132571 24.502732830027615 >500 0.035913811118974834 4.39080839739325 >1k 0.02020151875442334 7.874102999637704 >5k 0.0037410219915598782 5.645604360342485 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9942 1.3886173047806873 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9861 1.3773038867876037 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8860 1.2374923878854245 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6456 0.9017213155968736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5311 0.7417970735958792 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4893 0.6834142498784855 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3735 0.5216742741255147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3686 0.5148303545988345 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3006 0.4198535121877636 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2782 0.38856702292294026 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2768 0.3866116173438888 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2629 0.3671972333804493 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2552 0.35644250269566624 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2449 0.34205630450693053 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2343 0.32725109083696946 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2312 0.3229212641976412 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2286 0.3192897966936885 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1968 0.27487415568380535 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.24680011844170935 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1668 0.23297260756127403 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1598 0.22319557966601675 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1521 0.2124408489812337 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1501 0.2096474124397316 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1474 0.20587627310870377 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1473 0.20573660128162868 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1386 0.1935851523260946 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1241 0.1733327374002045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1131 0.157968836421943 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 0.1574101491136426 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1043 0.14567771563933382 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1040 0.14525870015810852 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1010 0.1410685453458554 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 994 0.1388337961126537 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 979 0.13673871870652712 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 909 0.12696169081126985 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 877 0.12249219234486651 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 877 0.12249219234486651 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 876 0.1223525205177914 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 802 0.11201680531423368 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 776 0.10838533781028095 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 767 0.10712829136660501 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 752 0.10503321396047845 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 732 0.10223977741897637 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 731 0.10210010559190126 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 731 0.10210010559190126 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.586873083004173E-4 0.0 18-19 0.0 0.0 0.0 0.001187210530138387 0.0 20-21 0.0 0.0 0.0 0.002234749233201669 0.0 22-23 0.0 0.0 0.0 0.005237693515316413 0.0 24-25 0.0 0.0 0.0 0.008240637797431156 0.0 26-27 0.0 0.0 0.0 0.013687839053360224 0.0 28-29 0.0 0.0 0.0 0.03324189484387483 0.0 30-31 0.0 0.0 0.0 0.08464112720751323 0.0 32-33 0.0 0.0 0.0 0.18869663837846595 0.0 34-35 0.0 0.0 0.0 0.3410786017174048 0.0 36-37 0.0 0.0 0.0 0.5610617293606941 0.0 38-39 0.0 0.0 0.0 0.8497633959249348 0.0 40-41 0.0 0.0 0.0 1.153968635294512 0.0 42-43 0.0 0.0 0.0 1.4557296176902748 0.0 44-45 0.0 0.0 0.0 1.817828829382483 0.0 46-47 0.0 0.0 0.0 2.2429898709991005 0.0 48-49 0.0 0.0 0.0 2.6490158723064288 0.0 50-51 0.0 0.0 0.0 3.081439848930952 0.0 52-53 0.0 0.0 0.0 3.4810409461928256 0.0 54-55 0.0 0.0 0.0 3.9193311395545027 0.0 56-57 0.0 0.0 0.0 4.498969221916186 0.0 58-59 0.0 0.0 0.0 5.076442390958205 0.0 60-61 0.0 0.0 0.0 5.7055941360180125 0.0 62-63 0.0 0.0 0.0 6.301992837628708 0.0 64-65 0.0 0.0 0.0 6.908308238961736 0.0 66-67 0.0 0.0 0.0 7.616723745886665 0.0 68-69 0.0 0.0 0.0 8.39085484745043 0.0 70-71 0.0 0.0 0.0 9.248370029778034 0.0 72-73 0.0 0.0 0.0 10.054066964260773 0.0 74-75 0.0 0.0 0.0 10.967520713331956 0.0 76-77 0.0 0.0 0.0 11.854017799777642 0.0 78-79 0.0 0.0 0.0 12.932912828019287 0.0 80-81 0.0 0.0 0.0 14.058248738763403 0.0 82-83 0.0 0.0 0.0 15.097826147683403 0.0 84-85 0.0 0.0 0.0 16.20723947014096 0.0 86-87 0.0 0.0 0.0 17.343050767915706 0.0 88-89 0.0 0.0 0.0 18.494784653977014 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 75 2.3301254E-9 50.798702 1 GTAAGCG 100 0.0 42.717728 94-95 GGTGTAC 45 9.65847E-4 42.19035 8 TGCCGTT 25 0.0016219553 37.971313 94-95 CGCAGAA 215 0.0 35.322147 9 CATATAG 205 0.0 32.41453 3 GACTGAA 60 0.0039645955 31.64276 7 ATGTATT 110 2.6766775E-6 30.306044 1 CTTTGCG 270 0.0 29.884829 2 GATCCTC 80 4.511879E-4 29.665087 5 TGACCGT 65 0.005864221 29.2087 5 CTCCGAC 155 0.0 29.090923 94-95 GCTTTGC 285 0.0 28.407167 1 CCCTATA 220 1.8189894E-12 28.046993 2 GTACCCT 85 6.433149E-4 27.920082 8 CGTGCAA 70 0.008420457 27.122366 9 TAGACCC 70 0.008420457 27.122366 5 GACCGTG 70 0.008420457 27.122366 6 AGGGTGT 110 9.520488E-5 25.889532 6 TGGGATA 165 8.462666E-8 25.889528 6 >>END_MODULE