##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939036_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 718097 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.895445879874167 31.0 31.0 34.0 30.0 34.0 2 32.1347673085948 33.0 31.0 34.0 30.0 34.0 3 32.21243926656148 34.0 31.0 34.0 30.0 34.0 4 35.85050766122126 37.0 35.0 37.0 35.0 37.0 5 35.76416556537627 37.0 35.0 37.0 33.0 37.0 6 35.83648727121823 37.0 35.0 37.0 35.0 37.0 7 35.78450543589515 37.0 35.0 37.0 33.0 37.0 8 35.76773750621434 37.0 35.0 37.0 33.0 37.0 9 37.37337295657829 39.0 37.0 39.0 34.0 39.0 10-11 37.28782741050304 39.0 37.0 39.0 33.5 39.0 12-13 37.25275972466115 39.0 37.0 39.0 33.0 39.0 14-15 38.35204784311869 40.0 38.0 41.0 33.0 41.0 16-17 38.31936771773173 40.0 38.0 41.0 33.5 41.0 18-19 38.41321297819097 40.0 38.0 41.0 33.5 41.0 20-21 38.41739695333639 40.0 38.0 41.0 34.0 41.0 22-23 38.32246757749998 40.0 38.0 41.0 34.0 41.0 24-25 38.221681054230835 40.0 37.0 41.0 33.0 41.0 26-27 38.001400924944676 40.0 37.0 41.0 33.0 41.0 28-29 37.81714378419629 40.0 37.0 41.0 33.0 41.0 30-31 37.623197144675444 40.0 36.0 41.0 33.0 41.0 32-33 37.35835687936309 39.0 36.0 41.0 32.0 41.0 34-35 37.22159750005918 39.0 35.0 41.0 32.0 41.0 36-37 37.053993402005574 39.5 35.0 41.0 31.0 41.0 38-39 36.88607667209305 39.0 35.0 41.0 31.0 41.0 40-41 36.76153848296261 39.0 35.0 41.0 31.0 41.0 42-43 36.56204941672225 39.0 35.0 41.0 30.5 41.0 44-45 36.27789490834804 39.0 35.0 41.0 30.0 41.0 46-47 35.91851031267363 39.0 35.0 41.0 29.5 41.0 48-49 35.579520594014454 39.0 35.0 40.5 27.5 41.0 50-51 35.3339653278039 39.0 35.0 40.0 27.0 41.0 52-53 35.15863246887259 38.5 35.0 40.0 26.5 41.0 54-55 34.97365328082418 38.0 35.0 40.0 26.0 41.0 56-57 34.69371477669452 38.0 34.0 40.0 24.5 41.0 58-59 34.43580393735108 38.0 34.0 40.0 24.0 41.0 60-61 33.99392004144287 38.0 33.0 40.0 22.5 41.0 62-63 33.71488113722798 37.0 33.0 40.0 22.0 41.0 64-65 33.474753410750914 37.0 33.0 39.5 22.0 41.0 66-67 32.97691467865762 36.5 32.5 39.0 20.5 40.5 68-69 32.51322801794187 36.0 32.0 39.0 18.5 40.0 70-71 32.0019133905308 35.0 31.5 38.0 16.0 40.0 72-73 31.420177914682835 35.0 31.0 37.5 11.5 39.5 74-75 30.903945427985356 35.0 30.5 37.0 7.5 39.0 76-77 28.865225032272797 32.5 28.0 35.0 4.5 37.5 78-79 29.836700334355942 34.0 29.0 36.0 2.0 38.0 80-81 29.762718685637175 34.0 29.5 36.0 2.0 37.0 82-83 29.350781301133413 34.0 29.0 35.0 2.0 37.0 84-85 28.86096168066431 34.0 29.0 35.0 2.0 36.5 86-87 28.26071965208043 34.0 28.5 35.0 2.0 36.0 88-89 27.570917995758236 33.0 26.5 35.0 2.0 35.5 90-91 27.00765565097751 33.0 25.5 35.0 2.0 35.0 92-93 26.591396426945103 33.0 25.0 35.0 2.0 35.0 94-95 26.101652701515256 32.0 24.5 34.5 2.0 35.0 96-97 25.33655341827079 32.0 21.5 34.0 2.0 35.0 98-99 24.33886786882552 32.0 9.5 34.0 2.0 35.0 100-101 20.070770383388314 27.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21635557031749642 1101 2 0.25596719645331945 1101 3 0.24668228361187516 1101 4 0.20087688867582898 1101 5 0.22303310085091965 1101 6 0.13390449314626096 1101 7 0.2059069043743662 1101 8 0.276457431424177 1101 9 0.41839851614248147 1101 10-11 0.389208695097615 1101 12-13 0.29673545434553006 1101 14-15 0.36669097235162695 1101 16-17 0.4496665660964254 1101 18-19 0.31583488995286757 1101 20-21 0.20746873583117775 1101 22-23 0.3130745996191706 1101 24-25 0.4338314429031911 1101 26-27 0.5359067544676321 1101 28-29 0.31863841823750505 1101 30-31 0.4467063130190567 1101 32-33 0.5348162890915233 1101 34-35 0.7089580367872514 1101 36-37 0.750002843097981 1101 38-39 0.6723598229512362 1101 40-41 0.5308143261138341 1101 42-43 0.532261036261886 1101 44-45 0.8202449501759617 1101 46-47 0.8677253012233166 1101 48-49 0.6105228041737263 1101 50-51 0.7330688567684547 1101 52-53 0.6961301235712156 1101 54-55 0.6414637098648583 1101 56-57 0.5017781693761734 1101 58-59 0.45413073789299574 1101 60-61 0.6213848168028235 1101 62-63 0.47764301666291686 1101 64-65 0.418400569781717 1101 66-67 0.38167694102055094 1101 68-69 0.5059863818999943 1101 70-71 0.614655186978446 1101 72-73 0.6439459047890033 1101 74-75 0.5803735152184011 1101 76-77 0.6253941396862146 1101 78-79 0.5861152406922692 1101 80-81 0.9795336136275132 1101 82-83 0.6706121377834542 1101 84-85 0.5756927300723085 1101 86-87 0.8215691483944205 1101 88-89 0.9007312069842186 1101 90-91 0.6450449261881559 1101 92-93 0.5298547978534565 1101 94-95 0.5777385103623338 1101 96-97 0.5253335784221633 1101 98-99 0.6085459089951542 1101 100-101 1.1738532796899364 1102 1 0.05728846362730522 1102 2 0.050709256712963224 1102 3 0.059207593497980326 1102 4 0.23542401044721828 1102 5 0.15459749618319307 1102 6 0.1324661934120499 1102 7 0.21489744138614242 1102 8 0.14680099421829595 1102 9 0.19810824956828554 1102 10-11 0.2935233615656827 1102 12-13 0.21863438237003407 1102 14-15 0.3329025653267621 1102 16-17 0.23770587031310697 1102 18-19 0.20072203695268342 1102 20-21 0.04592416948552369 1102 22-23 0.34908096723741266 1102 24-25 0.30403885596519586 1102 26-27 0.46702895408873246 1102 28-29 0.2589233784631588 1102 30-31 0.21346785781813793 1102 32-33 0.3697446301128622 1102 34-35 0.3869615929717014 1102 36-37 0.6425271754988202 1102 38-39 0.5865552362180395 1102 40-41 0.5183467344047727 1102 42-43 0.4590020073236687 1102 44-45 0.3929174879604176 1102 46-47 0.24432709700337796 1102 48-49 0.31770894994935617 1102 50-51 0.445124973731545 1102 52-53 0.6399596315237588 1102 54-55 0.5766273455514508 1102 56-57 0.5069297494112774 1102 58-59 0.5186180003291696 1102 60-61 0.12871944724357576 1102 62-63 0.4926555610570347 1102 64-65 0.47510286035549854 1102 66-67 0.37948109021272813 1102 68-69 0.4748197299731842 1102 70-71 0.3576044927311841 1102 72-73 0.33637900523259745 1102 74-75 0.3157380008516917 1102 76-77 -0.15377797684864447 1102 78-79 -0.04260443980969697 1102 80-81 0.27678455271156466 1102 82-83 0.46642361346036054 1102 84-85 0.4678132679684808 1102 86-87 0.4924172797684392 1102 88-89 0.6620479261713221 1102 90-91 0.8174570339721967 1102 92-93 0.5672761917474283 1102 94-95 0.476524566767889 1102 96-97 -0.018812681300321543 1102 98-99 0.40593183413839995 1102 100-101 0.930187151415943 1103 1 0.13282312506942873 1103 2 0.24010666397117575 1103 3 0.2509166790804329 1103 4 0.23613353547742832 1103 5 0.18377499645784923 1103 6 0.12956400715943772 1103 7 0.16195314138980876 1103 8 0.29499559633497086 1103 9 0.2562114831713913 1103 10-11 0.30846458542769994 1103 12-13 0.17044161128569613 1103 14-15 0.4273655235426048 1103 16-17 0.23771731426521114 1103 18-19 0.35388102549042344 1103 20-21 0.4021194673944848 1103 22-23 0.38193426492664884 1103 24-25 0.46321736330763486 1103 26-27 0.4151123209909997 1103 28-29 0.49748858152464237 1103 30-31 0.4240617531563302 1103 32-33 0.368163075932614 1103 34-35 0.5986838619869701 1103 36-37 0.5959640231935524 1103 38-39 0.3259581365732558 1103 40-41 0.41673588370715464 1103 42-43 0.3190836713658527 1103 44-45 0.43922200694822777 1103 46-47 0.5849975294932079 1103 48-49 0.5780268171628933 1103 50-51 0.6532080659789585 1103 52-53 0.9358704785593162 1103 54-55 0.681437925256283 1103 56-57 0.48605140319968143 1103 58-59 0.4156544744933015 1103 60-61 0.46704386955286736 1103 62-63 0.4716673529052784 1103 64-65 0.5745485153157617 1103 66-67 0.6884861072413386 1103 68-69 0.37267787989811296 1103 70-71 0.5982296071707012 1103 72-73 0.6562443328042811 1103 74-75 0.3404844028731553 1103 76-77 0.6360423988791908 1103 78-79 0.4832016721762393 1103 80-81 0.26477526937762264 1103 82-83 0.44277583084052097 1103 84-85 0.5667073244375196 1103 86-87 0.6291290203477793 1103 88-89 0.6362463917662247 1103 90-91 0.5014963828570984 1103 92-93 0.8025524029753228 1103 94-95 0.7028630514055259 1103 96-97 0.874721943521152 1103 98-99 0.9126059470032359 1103 100-101 1.4216980277480182 1104 1 -0.013261212279971346 1104 2 -0.05658008301677597 1104 3 -0.02468573055812584 1104 4 -0.04699899464305446 1104 5 -0.04363464209636447 1104 6 -0.039110691635741546 1104 7 -0.06093842487525336 1104 8 -0.04385982535178101 1104 9 -0.02993309514186393 1104 10-11 -0.05298119770483112 1104 12-13 0.08610426188810294 1104 14-15 0.015467933181156468 1104 16-17 5.18519084486968E-4 1104 18-19 -0.09867921547343883 1104 20-21 -0.09260944826816342 1104 22-23 0.05211498781821433 1104 24-25 -0.16630070898153093 1104 26-27 0.1026123209909997 1104 28-29 0.06525966586199416 1104 30-31 0.145447295324999 1104 32-33 0.13698837713742762 1104 34-35 0.14537060897492893 1104 36-37 0.32789173403692473 1104 38-39 0.3605966907901248 1104 40-41 0.20062142587583054 1104 42-43 0.1368547557032045 1104 44-45 0.21181236839400697 1104 46-47 0.011955360818511451 1104 48-49 0.21356898583758976 1104 50-51 0.18859963224401355 1104 52-53 0.27547891229425403 1104 54-55 0.3967993710394211 1104 56-57 0.13891284898281242 1104 58-59 0.09487134196317726 1104 60-61 0.030296881601067582 1104 62-63 0.3195589191703405 1104 64-65 0.451807551460341 1104 66-67 0.37598610724133863 1104 68-69 -0.01587633696934887 1104 70-71 -0.07872822415460234 1104 72-73 -0.08622554671378779 1104 74-75 0.02572536672857595 1104 76-77 -0.20281302280755753 1104 78-79 0.15940649145334973 1104 80-81 0.21959454648605714 1104 82-83 0.4006071561417279 1104 84-85 0.12770130034113336 1104 86-87 0.1464482974562138 1104 88-89 0.3711861508023695 1104 90-91 0.6543578286402294 1104 92-93 0.6158054150235124 1104 94-95 0.6034654610440775 1104 96-97 0.6066496543645243 1104 98-99 0.9540216096538359 1104 100-101 1.8697401964227147 1105 1 -0.026685333361662344 1105 2 -0.07398645815128191 1105 3 -0.02732954152673983 1105 4 -0.00791954360316538 1105 5 0.13784456903806586 1105 6 0.03774284255960225 1105 7 0.04264869079092648 1105 8 0.10678888956645949 1105 9 0.007408805403215979 1105 10-11 -0.10605586827583835 1105 12-13 0.04806337212112766 1105 14-15 0.10622738386824437 1105 16-17 -0.02518091114006893 1105 18-19 0.045753566900572196 1105 20-21 0.059916835490589904 1105 22-23 -0.07103500600774737 1105 24-25 0.08621631372128746 1105 26-27 0.05431150425405917 1105 28-29 0.13085660706752122 1105 30-31 -0.005453893618181382 1105 32-33 0.19557337599082558 1105 34-35 0.13027288278465932 1105 36-37 0.04163690505775719 1105 38-39 0.03655234356227055 1105 40-41 0.09125056027580314 1105 42-43 0.12197312081813294 1105 44-45 -0.05836240096159884 1105 46-47 -0.19475802273032627 1105 48-49 -0.0725359022359342 1105 50-51 -0.02286445249905711 1105 52-53 0.18696306265852058 1105 54-55 -0.09129465954852378 1105 56-57 0.019843232302328317 1105 58-59 -0.08452854161211576 1105 60-61 -0.18760914955137054 1105 62-63 -0.15959047489200628 1105 64-65 0.025810003435147166 1105 66-67 -0.1976337749227568 1105 68-69 -0.07241177594340087 1105 70-71 -0.06738894316534427 1105 72-73 0.05350063473234812 1105 74-75 0.06092840435492164 1105 76-77 -0.08129914886375644 1105 78-79 0.2395774681860523 1105 80-81 0.08794753383809706 1105 82-83 0.0056980908909700645 1105 84-85 -0.07644214018524664 1105 86-87 0.22568104102198205 1105 88-89 -0.08875867976440688 1105 90-91 0.25658665610234976 1105 92-93 0.20370496343678823 1105 94-95 0.1149381453390923 1105 96-97 -0.009497217162579119 1105 98-99 0.05522179840297525 1105 100-101 0.40812286325402525 1106 1 0.12570876454362434 1106 2 0.03491806817830678 1106 3 0.07604096476944022 1106 4 0.07385667473047874 1106 5 -0.19425253215882066 1106 6 -0.028650348058789632 1106 7 -0.0033025952129932534 1106 8 -0.008062450831673118 1106 9 -0.21908397177615058 1106 10-11 0.10891307542292594 1106 12-13 0.0328781415477124 1106 14-15 -0.039922129271289464 1106 16-17 -0.043333098840960815 1106 18-19 0.03761485950489174 1106 20-21 -0.2865979573706525 1106 22-23 0.014948701527686126 1106 24-25 0.09527090788240855 1106 26-27 0.07070716733673521 1106 28-29 0.041196452656755866 1106 30-31 0.14474293603763044 1106 32-33 0.3991980086148814 1106 34-35 0.15199401891423747 1106 36-37 0.19548545694618014 1106 38-39 0.3425907463073443 1106 40-41 0.35674592386779125 1106 42-43 0.50370762975448 1106 44-45 0.6281506289251197 1106 46-47 0.7561280869653899 1106 48-49 1.0083781785556027 1106 50-51 0.958302894632773 1106 52-53 0.6381609178495111 1106 54-55 0.4803638049866663 1106 56-57 0.38545297424155933 1106 58-59 0.47824625129212706 1106 60-61 0.7252418597515486 1106 62-63 0.6475669866826834 1106 64-65 0.4921694395412999 1106 66-67 0.787122945342638 1106 68-69 0.5602552820176072 1106 70-71 0.7268719297650392 1106 72-73 0.62467643124209 1106 74-75 0.7400960653822217 1106 76-77 0.45712187095250556 1106 78-79 0.6341236419938383 1106 80-81 0.7572285627272315 1106 82-83 0.9966123221803294 1106 84-85 0.9113546361513336 1106 86-87 0.7382228847923393 1106 88-89 0.9759145357076306 1106 90-91 1.451365241551784 1106 92-93 1.5806482666060582 1106 94-95 1.5753109929514473 1106 96-97 1.3249369002644222 1106 98-99 1.379482262839268 1106 100-101 1.7209003436194337 1107 1 -0.1314679447894065 1107 2 0.04860029514824049 1107 3 -0.08049480376080709 1107 4 -0.07303662154551205 1107 5 -0.08352165797102629 1107 6 -0.05995372040317193 1107 7 -0.0498511573502185 1107 8 0.019106632755331532 1107 9 0.20330673111595843 1107 10-11 -0.04833169842013518 1107 12-13 0.09917801613254795 1107 14-15 0.29681758069826714 1107 16-17 0.1556390010121973 1107 18-19 0.004036367890066117 1107 20-21 0.07323957012300042 1107 22-23 0.09357266401657682 1107 24-25 -0.09737371344573376 1107 26-27 0.08460682260259489 1107 28-29 0.2109260222747693 1107 30-31 0.2974469247439657 1107 32-33 0.015534751475982489 1107 34-35 -0.005402009395552909 1107 36-37 0.15870856358688457 1107 38-39 0.17418150213852357 1107 40-41 0.2430120182883897 1107 42-43 0.17749586089650649 1107 44-45 0.36349312575822523 1107 46-47 0.4183642582106586 1107 48-49 0.29038267837598397 1107 50-51 0.4028870393833017 1107 52-53 0.3155382914417473 1107 54-55 0.28153035506134927 1107 56-57 0.34839562989186135 1107 58-59 0.5678361626191943 1107 60-61 0.6726021541935765 1107 62-63 0.6359834154386732 1107 64-65 0.5633976888338665 1107 66-67 0.6829747915924713 1107 68-69 0.7211931857567464 1107 70-71 0.5046665135946782 1107 72-73 0.8213608072859024 1107 74-75 0.7624943309976686 1107 76-77 0.2408500414667074 1107 78-79 0.4727855441448021 1107 80-81 0.6419632056721554 1107 82-83 0.9699316644754923 1107 84-85 0.7983549449625365 1107 86-87 0.7652690741251185 1107 88-89 0.6031731115207819 1107 90-91 0.6412022018411889 1107 92-93 0.9310481456024853 1107 94-95 0.707768941058557 1107 96-97 0.7802065428627252 1107 98-99 0.8319442443988265 1107 100-101 1.7164036743688378 1108 1 0.05077552538800134 1108 2 -0.029395394897214544 1108 3 -0.11809523354899198 1108 4 -0.006027090147433967 1108 5 -0.01937939325289051 1108 6 -0.02415212318472726 1108 7 -0.00784419140127568 1108 8 0.1292821094761436 1108 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70-71 -0.9718938619122426 2316 72-73 -0.7352819257126662 2316 74-75 -1.115081663043405 2316 76-77 -1.0181428189697748 2316 78-79 -1.2347406401663967 2316 80-81 -1.1157020709162566 2316 82-83 -1.137315490762301 2316 84-85 -1.424786395424217 2316 86-87 -1.6283468690421294 2316 88-89 -1.8443451005311822 2316 90-91 -1.7467495524781071 2316 92-93 -2.21868929344436 2316 94-95 -2.3698795127116306 2316 96-97 -2.536616444268496 2316 98-99 -2.838347097761421 2316 100-101 -3.8074852342254957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 18.0 5 78.0 6 224.0 7 294.0 8 435.0 9 723.0 10 1121.0 11 1658.0 12 2301.0 13 3117.0 14 3656.0 15 4823.0 16 6196.0 17 8313.0 18 7918.0 19 5415.0 20 5015.0 21 5378.0 22 5745.0 23 6578.0 24 7665.0 25 8584.0 26 10042.0 27 11943.0 28 14383.0 29 16815.0 30 20440.0 31 23982.0 32 28895.0 33 35920.0 34 48529.0 35 69585.0 36 93216.0 37 145826.0 38 107008.0 39 6254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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18.814285833651308 18-19 16.155895373466258 33.20101601872727 33.70248030558546 16.94060830222101 20-21 17.566359419409913 31.183322030310666 32.82035713838103 18.429961411898393 22-23 17.285687031139247 30.66424173892942 33.13417268140655 18.915898548524783 24-25 18.46407936532251 31.529097043992664 32.90815864709085 17.09866494359397 26-27 17.9552344599685 30.146902159457568 35.15193629829953 16.7459270822744 28-29 16.36122975029836 31.63249533141066 34.781303918551394 17.22497099973959 30-31 17.111267697817983 31.07901857269979 33.62484455442649 18.184869175055738 32-33 17.234579729479442 31.894368031059873 33.570952113711655 17.30010012574903 34-35 18.361141322890656 30.340115781132717 33.73549428405509 17.56324861192153 36-37 18.118843940296014 30.06514238363328 34.14164203372363 17.674371642347076 38-39 17.13576804586047 31.293140428187026 34.13741427624359 17.433677249708914 40-41 16.667525435813737 31.34628078687392 34.486242518263936 17.49995125904841 42-43 16.53511453251877 31.557402731958117 32.85911434869856 19.04836838682455 44-45 17.132299388239094 30.809883427030837 32.88023384222891 19.17758334250116 46-47 18.36404312251817 31.55278525707307 32.37231641840512 17.71085520200364 48-49 17.692276627122112 31.15028276281763 32.683371095387365 18.47406951467289 50-51 16.255842760547257 31.32408982445781 33.82997523289228 18.590092182102648 52-53 17.00766052497086 32.53773515277184 32.29229477354731 18.16230954870999 54-55 16.57603359991756 30.9902422653207 32.79313240411811 19.64059173064363 56-57 17.886441525309255 31.23596115845074 31.539402058496275 19.33819525774373 58-59 18.34726833181079 30.46131714979613 32.29909371448943 18.892320803903655 60-61 17.325891250051352 31.946089459578587 32.34611536203911 18.381903928330956 62-63 17.164742367674563 31.254760847072195 32.83644131642382 18.74405546882942 64-65 17.00723233501997 31.982420141081707 32.18441305427071 18.825934469627605 66-67 17.144108494799678 31.03530871538776 31.11872554678758 20.701857243024982 68-69 16.45159156503605 31.088489135997605 32.019092513743196 20.440826785223145 70-71 18.057518691764482 31.023594305504687 31.408918293768114 19.509968708962717 72-73 17.675676127319846 29.675935145251962 32.42173411112984 20.226654616298354 74-75 16.538573479627406 32.273773598831355 31.039121455736478 20.14853146580476 76-77 17.258338985957558 30.49259047277782 31.68846035064628 20.560610190618338 78-79 17.021934362627892 30.404318636618733 32.04922176251955 20.52452523823383 80-81 17.6967735556617 30.66298842635466 30.940040133853785 20.70019788412986 82-83 17.297036472788495 30.189932557857784 31.847229552553486 20.665801416800235 84-85 16.67608972046952 30.147668072697698 31.507721101745307 21.668521105087475 86-87 16.84123772296682 30.69575548988901 31.245615967847872 21.217390819296295 88-89 17.321610633504097 29.84232675032831 30.813344465842356 22.022718150325236 90-91 17.161603898399093 29.05190274678987 31.516064271054972 22.270429083756067 92-93 17.8397904461375 30.30008480748422 31.04288139346077 20.8172433529175 94-95 17.242935146644534 29.121483587871833 31.401328789843152 22.234252475640478 96-97 16.548135130129385 29.136198236161608 31.5798232262395 22.735843407469506 98-99 17.174211840461666 29.453054392373172 30.38029681226909 22.992436954896064 100-101 16.762080888793577 28.664720782846885 31.539123544590776 23.034074783768766 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10803.0 1 6499.0 2 2041.0 3 1712.5 4 1325.5 5 1357.5 6 2188.5 7 3566.0 8 4659.5 9 4689.5 10 3656.5 11 2644.0 12 2357.0 13 2217.5 14 2043.0 15 1959.0 16 1898.5 17 1868.0 18 1860.5 19 1879.5 20 2028.0 21 2341.0 22 2617.5 23 2891.5 24 3227.0 25 3870.5 26 5484.0 27 7191.5 28 7560.0 29 8244.5 30 10796.0 31 13351.5 32 17549.0 33 23886.5 34 28633.5 35 31467.0 36 36193.0 37 39968.5 38 38450.5 39 35920.0 40 33368.5 41 29822.5 42 25981.0 43 24737.5 44 24471.0 45 23108.5 46 22324.5 47 26424.0 48 39981.5 49 38604.0 50 21987.5 51 11467.5 52 5320.5 53 2728.0 54 1840.5 55 1506.0 56 1216.0 57 1013.5 58 944.5 59 831.0 60 662.5 61 566.5 62 454.0 63 313.0 64 218.5 65 167.0 66 126.5 67 86.5 68 59.0 69 45.0 70 47.5 71 46.0 72 32.0 73 28.5 74 24.0 75 14.5 76 6.5 77 4.5 78 4.0 79 3.5 80 6.0 81 5.0 82 2.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3865773008381876 2 0.0 3 0.0 4 0.0 5 2.78513905503017E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.051701928848052E-4 16-17 0.0020888542912726273 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002506625149527153 36-37 0.007589503924957213 38-39 0.01295089660589029 40-41 0.0020888542912726273 42-43 5.57027811006034E-4 44-45 8.355417165090511E-4 46-47 4.8739933463027977E-4 48-49 5.57027811006034E-4 50-51 0.0010444271456363137 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.392569527515085E-4 60-61 0.0025762536259029073 62-63 0.0 64-65 3.481423818787713E-4 66-67 0.0022977397203998904 68-69 0.0020192258148968734 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12233723299220021 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14009249446801755 88-89 0.4280758727581371 90-91 0.3225191025724937 92-93 0.0 94-95 0.0 96-97 5.57027811006034E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 718097.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.755754217204306 #Duplication Level Percentage of deduplicated Percentage of total 1 72.25368036350437 13.551722701863289 2 10.644208835245077 3.9928032950090246 3 4.201430646063416 2.3640300167458594 4 2.2489364735015203 1.6872199898840283 5 1.41464574635841 1.326637396155594 6 1.0838342887970502 1.2196877719713544 7 0.775659476711986 1.018365495101872 8 0.5754247510019267 0.8634020160230498 9 0.47723891800386975 0.8055878264068591 >10 4.670304999380313 18.933371456148798 >50 0.898676694174014 11.923667912765326 >100 0.6979796656984276 24.533958191482167 >500 0.034936149401305745 4.365802921781051 >1k 0.018583058192183907 7.0556896078051174 >5k 0.0037166116384367816 4.95134889883615 >10k+ 7.433223276873563E-4 1.4067045020204634 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10090 1.4051026532627207 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9729 1.3548308933194262 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8807 1.2264359828825353 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6503 0.9055879637430597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5364 0.7469742945590916 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5112 0.7118815424657114 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3717 0.5176180933773571 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3691 0.5139974126058179 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3058 0.425847761514113 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2873 0.4000852252550839 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2763 0.384766960452418 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2630 0.3662457857364674 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2504 0.3486994096897773 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2480 0.3453572428237411 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2456 0.34201507595770486 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2378 0.33115303364308724 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2335 0.3251649846747724 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1946 0.27099403005443556 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1750 0.24369966731513987 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1702 0.23701533358306748 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1577 0.2196082144891289 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1522 0.21194908208779592 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1494 0.2080498874107537 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1452 0.20220109539519035 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1421 0.19788412985989356 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1384 0.19273162260808777 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1191 0.16585503072704663 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1190 0.1657157737742951 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1037 0.1444094600033143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.1441309460978113 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1023 0.14245986266479319 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 953 0.13271187597218762 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 935 0.13020525082266043 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 934 0.13006599386990894 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 917 0.12769862567313328 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 892 0.12421720185434558 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 879 0.12240686146857598 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 856 0.11920395155529129 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 835 0.1162795555475096 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 794 0.11057002048469776 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 789 0.10987373572094021 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 762 0.10611379799664948 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 740 0.10305014503611629 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 731 0.10179683246135272 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 720 0.10026500598108612 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.392569527515085E-4 0.0 16-17 0.0 0.0 0.0 6.962847637575425E-4 0.0 18-19 0.0 0.0 0.0 0.001114055622012068 0.0 20-21 0.0 0.0 0.0 0.002506625149527153 0.0 22-23 0.0 0.0 0.0 0.005639906586436094 0.0 24-25 0.0 0.0 0.0 0.009051701928848053 0.0 26-27 0.0 0.0 0.0 0.015248636326290182 0.0 28-29 0.0 0.0 0.0 0.03460535275874986 0.0 30-31 0.0 0.0 0.0 0.09225773119787438 0.0 32-33 0.0 0.0 0.0 0.19725747357251178 0.0 34-35 0.0 0.0 0.0 0.3642265599215705 0.0 36-37 0.0 0.0 0.0 0.5999189524534987 0.0 38-39 0.0 0.0 0.0 0.9084427312744656 0.0 40-41 0.0 0.0 0.0 1.21487765580416 0.0 42-43 0.0 0.0 0.0 1.5278576571131754 0.0 44-45 0.0 0.0 0.0 1.8989774361959455 0.0 46-47 0.0 0.0 0.0 2.343973028713391 0.0 48-49 0.0 0.0 0.0 2.7595157757238926 0.0 50-51 0.0 0.0 0.0 3.20332768414295 0.0 52-53 0.0 0.0 0.0 3.615876406669294 0.0 54-55 0.0 0.0 0.0 4.072708840170618 0.0 56-57 0.0 0.0 0.0 4.64895411065636 0.0 58-59 0.0 0.0 0.0 5.222135728181569 0.0 60-61 0.0 0.0 0.0 5.8581918598740845 0.0 62-63 0.0 0.0 0.0 6.4675802851146855 0.0 64-65 0.0 0.0 0.0 7.091451433441444 0.0 66-67 0.0 0.0 0.0 7.794977558742064 0.0 68-69 0.0 0.0 0.0 8.584494852366742 0.0 70-71 0.0 0.0 0.0 9.443013966079791 0.0 72-73 0.0 0.0 0.0 10.248963580129146 0.0 74-75 0.0 0.0 0.0 11.161444763033407 0.0 76-77 0.0 0.0 0.0 12.052550003690309 0.0 78-79 0.0 0.0 0.0 13.144533398691262 0.0 80-81 0.0 0.0 0.0 14.276553167608277 0.0 82-83 0.0 0.0 0.0 15.302250253099512 0.0 84-85 0.0 0.0 0.0 16.40286757917106 0.0 86-87 0.0 0.0 0.0 17.540248740769005 0.0 88-89 0.0 0.0 0.0 18.710007143881676 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACAGT 25 0.0050401627 56.943768 4 TTTCGAG 20 5.4130604E-4 47.45644 58-59 GTAAGCG 65 0.0 47.45314 94-95 TCAGGGC 55 4.9904265E-5 43.139214 3 GGGTATG 115 5.4569682E-11 41.2636 7 CAGGGCC 70 4.3372493E-6 40.674118 4 GGTATGG 60 8.335983E-5 39.544277 8 GGTATGC 75 6.965678E-6 37.962513 8 TGGGTAT 135 3.110472E-10 35.15047 6 CTTTGCG 230 0.0 35.07406 2 GGGAGCT 95 8.7612716E-7 34.96547 7 CTTCAGG 55 0.0025484182 34.629528 1 ATGCACT 55 0.0025914975 34.51137 8 CATATAG 290 0.0 32.7263 3 TGACCGT 75 3.095173E-4 31.635427 5 GGAGCTC 60 0.003969105 31.635424 8 CATTCCG 165 7.6397555E-11 31.635424 9 GTATGGT 90 2.4280365E-5 31.635424 3 GCCGTCC 30 0.0039704153 31.635424 92-93 TACCGCG 60 0.003969105 31.635424 8 >>END_MODULE