FastQCFastQC Report
Fri 27 May 2016
SRR939035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455144
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109440.24564862549897376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101920.22876926088135421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.1860321462112111No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54920.1232732320212321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.11979410766520679No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45180.10141086348724082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG124550.023.0447162
GTACATG131000.022.9357431
ACATGGG131550.020.4143073
GTATCAA262900.020.365681
GAGTACT109700.020.21833212-13
AGAGTAC176850.018.68435510-11
GGTATCA205100.018.5373331
GTACTTT118850.018.52328914-15
CATGGGG85750.018.5034684
CAACGCA286700.018.3537125
TCAACGC288800.018.2204594
ATCAACG290850.018.043183
AGTACTT117800.017.9841612-13
TATCAAC292150.017.9791052
AACGCAG293250.017.93536
ACGCAGA316600.016.4181447
CGCAGAG318300.016.1222368
GTGTAGC26650.016.029711
GAGTACA113450.015.1873681
TTTGCGT8450.015.1338513
Produced by FastQC (version 0.11.5)