##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939035_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4455144 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.794687219986603 33.0 31.0 34.0 30.0 34.0 2 31.89618046016021 33.0 31.0 34.0 30.0 34.0 3 31.948929821348088 34.0 31.0 34.0 30.0 34.0 4 35.47466097616598 37.0 35.0 37.0 33.0 37.0 5 35.50344837338591 37.0 35.0 37.0 33.0 37.0 6 35.577948995588024 37.0 35.0 37.0 33.0 37.0 7 35.50591092005107 37.0 35.0 37.0 33.0 37.0 8 35.4886764602895 37.0 35.0 37.0 33.0 37.0 9 37.10068002291284 39.0 37.0 39.0 33.0 39.0 10-11 36.98284443780044 39.0 37.0 39.0 33.0 39.0 12-13 36.87685639341848 39.0 37.0 39.0 33.0 39.0 14-15 38.09784161858741 40.0 38.0 41.0 33.0 41.0 16-17 38.004268099976116 40.0 38.0 41.0 33.0 41.0 18-19 37.90077907695015 40.0 38.0 41.0 33.0 41.0 20-21 38.011851581003896 40.0 38.0 41.0 33.0 41.0 22-23 37.95191681795246 40.0 38.0 41.0 33.0 41.0 24-25 37.87556631166131 40.0 38.0 41.0 32.0 41.0 26-27 37.758560553822726 40.0 38.0 41.0 32.0 41.0 28-29 37.63063853828294 40.0 37.5 41.0 32.0 41.0 30-31 37.44930118981564 40.0 37.0 41.0 31.5 41.0 32-33 37.261801189815635 40.0 37.0 41.0 31.0 41.0 34-35 36.94616414194468 40.0 36.5 41.0 30.5 41.0 36-37 36.69297602950657 40.0 36.0 41.0 30.0 41.0 38-39 36.45866328899807 39.0 35.5 41.0 30.0 41.0 40-41 36.319295852165496 39.0 35.5 41.0 30.0 41.0 42-43 36.1207363892166 39.0 35.0 41.0 29.0 41.0 44-45 36.07688550583326 39.0 35.0 41.0 29.0 41.0 46-47 35.904507688191444 39.0 35.0 41.0 28.0 41.0 48-49 35.689741678383456 39.0 35.0 41.0 27.0 41.0 50-51 34.60133937309321 38.0 34.0 40.0 25.5 40.5 52-53 34.711013156926015 38.0 34.5 39.5 26.0 40.5 54-55 35.20284866213079 39.0 35.0 40.0 26.0 41.0 56-57 35.097092147863236 39.0 35.0 40.5 26.0 41.0 58-59 34.88227260443209 38.0 34.0 40.0 26.0 41.0 60-61 34.56972479452965 38.0 34.0 40.0 25.5 41.0 62-63 34.187714471182076 37.5 34.0 40.0 23.5 41.0 64-65 33.80373182101409 37.0 33.5 40.0 23.0 41.0 66-67 33.385968781256004 36.5 33.0 39.0 22.0 41.0 68-69 32.937763291152876 36.0 32.5 39.0 21.5 41.0 70-71 32.4966674477862 35.0 32.0 39.0 20.0 40.0 72-73 32.05787815163775 35.0 31.5 38.0 18.5 40.0 74-75 31.587882456773563 35.0 31.0 37.0 17.5 39.0 76-77 31.150877390270665 35.0 31.0 36.5 13.5 39.0 78-79 30.74530396772809 35.0 31.0 36.0 9.5 39.0 80-81 30.28743335793411 34.0 30.0 36.0 7.0 37.5 82-83 29.82191417381795 34.0 30.0 35.0 2.0 37.0 84-85 29.395376557974334 34.0 29.0 35.0 2.0 36.5 86-87 28.947844334549007 34.0 29.0 35.0 2.0 36.0 88-89 28.462297178273026 34.0 29.0 35.0 2.0 36.0 90-91 27.995252342011838 34.0 27.5 35.0 2.0 35.0 92-93 27.480636203902726 33.5 26.5 35.0 2.0 35.0 94-95 26.93206179194208 33.0 25.5 35.0 2.0 35.0 96-97 26.271306943165023 33.0 24.5 35.0 2.0 35.0 98-99 25.375399538151854 32.5 21.0 35.0 2.0 35.0 100-101 22.068350764868654 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15612301722009292 1101 2 0.2877457279758495 1101 3 0.32026843011307093 1101 4 0.25459808295549635 1101 5 0.1379298292259108 1101 6 0.3014031960494066 1101 7 0.27522574510336284 1101 8 0.3006202412760146 1101 9 0.37950742781301017 1101 10-11 0.3177359871997325 1101 12-13 0.2943138151209652 1101 14-15 0.4055008234200912 1101 16-17 0.39852136831956386 1101 18-19 0.5282968951978404 1101 20-21 0.4491693308325395 1101 22-23 0.4905517339972718 1101 24-25 0.5257048948551883 1101 26-27 0.5397774646029134 1101 28-29 0.5348328886469673 1101 30-31 0.4734161189505812 1101 32-33 0.4144155494153168 1101 34-35 0.42435737304962373 1101 36-37 0.3650787603229517 1101 38-39 0.5901709429001656 1101 40-41 0.47941766763987914 1101 42-43 0.5313628485390112 1101 44-45 0.6290147113296953 1101 46-47 0.6930880042062952 1101 48-49 0.578254928016861 1101 50-51 0.4841743506540297 1101 52-53 0.5570055568046968 1101 54-55 0.6523825424997369 1101 56-57 0.6026623695170983 1101 58-59 0.5723647923064235 1101 60-61 0.6643033356650747 1101 62-63 0.6662977073719176 1101 64-65 0.3697649162237937 1101 66-67 0.44417265874529477 1101 68-69 0.41950797180734867 1101 70-71 0.4070086177130676 1101 72-73 0.2966789235706955 1101 74-75 0.1673389675597683 1101 76-77 0.0938689717641168 1101 78-79 0.10136134461970414 1101 80-81 0.08002955589294558 1101 82-83 0.15769039428965215 1101 84-85 0.12350224049093939 1101 86-87 0.1325369606958624 1101 88-89 0.07148684573896347 1101 90-91 0.01688258943595855 1101 92-93 0.15710612719991346 1101 94-95 -0.09346581787941588 1101 96-97 -0.14739841806193255 1101 98-99 -0.19991693960639267 1101 100-101 0.15960243705029242 1102 1 0.1764474363115056 1102 2 0.21473133083631168 1102 3 0.25251187649358897 1102 4 0.0579670706319817 1102 5 0.2346273377881687 1102 6 0.20505946322446533 1102 7 0.28581026660661735 1102 8 0.304314576175841 1102 9 0.33008130085148224 1102 10-11 0.40960318345036484 1102 12-13 0.4005630598208043 1102 14-15 0.409074727833719 1102 16-17 0.48820742471204426 1102 18-19 0.39534572436180326 1102 20-21 0.3920028460343943 1102 22-23 0.4322452156837855 1102 24-25 0.46865332304945184 1102 26-27 0.5249666919736455 1102 28-29 0.42502781305372395 1102 30-31 0.4657619533143418 1102 32-33 0.43958292631764095 1102 34-35 0.3779752325030188 1102 36-37 0.29203829675325466 1102 38-39 0.41501768481710144 1102 40-41 0.402580631501543 1102 42-43 0.2981399068355941 1102 44-45 0.48414870649983044 1102 46-47 0.5693608900249529 1102 48-49 0.5603649763095504 1102 50-51 0.46457979160676643 1102 52-53 0.4597930423891228 1102 54-55 0.4565912629076294 1102 56-57 0.4830496951956107 1102 58-59 0.5083897499887371 1102 60-61 0.5930715960344699 1102 62-63 0.5266101239757504 1102 64-65 0.4576292154621129 1102 66-67 0.3780824777249734 1102 68-69 0.5526431585175544 1102 70-71 0.4797507787263058 1102 72-73 0.4941028444890847 1102 74-75 0.3988161145776594 1102 76-77 0.3910399051534732 1102 78-79 0.3324379514123166 1102 80-81 0.40083230292486505 1102 82-83 0.4173606819831086 1102 84-85 0.22107336217893803 1102 86-87 0.20024868840628685 1102 88-89 0.10025577417247433 1102 90-91 0.10790510212291338 1102 92-93 0.22042762983357633 1102 94-95 0.0891558303325759 1102 96-97 0.10823205014722248 1102 98-99 -0.19522492253114976 1102 100-101 -0.02355106773931226 1103 1 0.28970066140076156 1103 2 0.3149043016676636 1103 3 0.3939376932352836 1103 4 0.3710965188628279 1103 5 0.1868756749207705 1103 6 0.27371253650031946 1103 7 0.2729617393644759 1103 8 0.21382556066283342 1103 9 0.36989047740678416 1103 10-11 0.36673013959633494 1103 12-13 0.39604606733370673 1103 14-15 0.5513860538181348 1103 16-17 0.5984958619062866 1103 18-19 0.6424144160266962 1103 20-21 0.6544018961486628 1103 22-23 0.562229680931587 1103 24-25 0.5666968106633732 1103 26-27 0.6329165242862658 1103 28-29 0.5671095395848837 1103 30-31 0.5609112467390176 1103 32-33 0.5577579126598735 1103 34-35 0.46198324939491187 1103 36-37 0.41933033165568645 1103 38-39 0.667354073487779 1103 40-41 0.6873758228793392 1103 42-43 0.5637026423892664 1103 44-45 0.6308812846587912 1103 46-47 0.6610975140986071 1103 48-49 0.6660153272509817 1103 50-51 0.5291896687447633 1103 52-53 0.628419775259232 1103 54-55 0.6537718052611368 1103 56-57 0.689338775859305 1103 58-59 0.6636652894263051 1103 60-61 0.7821140640731628 1103 62-63 0.7378694270382837 1103 64-65 0.5903499329329165 1103 66-67 0.637604442798434 1103 68-69 0.44858652918645703 1103 70-71 0.49639957583792693 1103 72-73 0.5112857188874855 1103 74-75 0.5715300427601271 1103 76-77 0.5578546639599828 1103 78-79 0.5606752327845861 1103 80-81 0.5145397330586654 1103 82-83 0.6528012694456713 1103 84-85 0.6713955460603778 1103 86-87 0.6208365315614337 1103 88-89 0.6492879341708466 1103 90-91 0.7368886378623003 1103 92-93 0.6404918857670516 1103 94-95 0.5132591159273474 1103 96-97 0.629172997758829 1103 98-99 0.42971949489850303 1103 100-101 0.6301379601608339 1104 1 0.29354324446562075 1104 2 0.3336933908082109 1104 3 0.2910720560563398 1104 4 0.3721795020454408 1104 5 0.32511130289874757 1104 6 0.33085408844706166 1104 7 0.3175179377474322 1104 8 0.3434173955141304 1104 9 0.4389626095316501 1104 10-11 0.3832320208585642 1104 12-13 0.41974053502924136 1104 14-15 0.5260277741879023 1104 16-17 0.5118219076748929 1104 18-19 0.49939107490129686 1104 20-21 0.5482712746261384 1104 22-23 0.5734163158024472 1104 24-25 0.511520271023052 1104 26-27 0.4907667911329483 1104 28-29 0.6241516527263968 1104 30-31 0.5462347943095267 1104 32-33 0.5972300425698691 1104 34-35 0.6499254752183674 1104 36-37 0.7497847462310645 1104 38-39 0.7727400575535839 1104 40-41 0.7440471746287685 1104 42-43 0.6646417969148715 1104 44-45 0.7561701416985542 1104 46-47 0.7614965613318034 1104 48-49 0.7313235506325313 1104 50-51 0.5546704588287525 1104 52-53 0.7501013800492728 1104 54-55 0.788462589798705 1104 56-57 0.7401161289136695 1104 58-59 0.6771631266899618 1104 60-61 0.7734692327222987 1104 62-63 0.8106657846709808 1104 64-65 0.8331940304207919 1104 66-67 0.6402208831014704 1104 68-69 0.7087973486821824 1104 70-71 0.7241282827827717 1104 72-73 0.7071434812513502 1104 74-75 0.7709389932677517 1104 76-77 0.6618573873373812 1104 78-79 0.6199453750259138 1104 80-81 0.7339716444709197 1104 82-83 0.9120896844326403 1104 84-85 0.967669544035612 1104 86-87 0.8178003487564602 1104 88-89 0.8389295022677423 1104 90-91 0.9238257473169682 1104 92-93 1.020287673991465 1104 94-95 1.0447219230456 1104 96-97 1.0791996916561963 1104 98-99 1.1963675350542466 1104 100-101 1.3152664830862086 1105 1 0.2277936506911793 1105 2 0.2003514935274744 1105 3 0.27177333545613536 1105 4 0.27338290523675823 1105 5 0.21653118290008422 1105 6 0.3074779398722356 1105 7 0.257896342843722 1105 8 0.29785770109195653 1105 9 0.2729507701553402 1105 10-11 0.3032888924373296 1105 12-13 0.31314729016305876 1105 14-15 0.46627774817013545 1105 16-17 0.41210286021961196 1105 18-19 0.44356624763567254 1105 20-21 0.521584238801637 1105 22-23 0.33742640552608805 1105 24-25 0.42741356940828723 1105 26-27 0.3303795377482217 1105 28-29 0.3849330387507095 1105 30-31 0.4688436266838565 1105 32-33 0.44375056243968203 1105 34-35 0.48337586040494074 1105 36-37 0.4770394301595928 1105 38-39 0.5600975526821301 1105 40-41 0.5994678779828462 1105 42-43 0.475015406425392 1105 44-45 0.5255610764567891 1105 46-47 0.47245605947999536 1105 48-49 0.577849661793465 1105 50-51 0.5160140702476212 1105 52-53 0.5054171525701463 1105 54-55 0.5265678906124123 1105 56-57 0.4709756502811686 1105 58-59 0.42804396576799775 1105 60-61 0.5879524973979429 1105 62-63 0.6471858448748549 1105 64-65 0.6615905065699721 1105 66-67 0.7025390256554473 1105 68-69 0.6661803311072063 1105 70-71 0.5494160291676451 1105 72-73 0.37748604251897433 1105 74-75 0.38312749091985765 1105 76-77 0.4508953323676259 1105 78-79 0.4749077891869504 1105 80-81 0.527859486655128 1105 82-83 0.6219876801767441 1105 84-85 0.7121494786618996 1105 86-87 0.6899391530360965 1105 88-89 0.7604047354892032 1105 90-91 0.7221452917958899 1105 92-93 0.7645295151457496 1105 94-95 0.6928226373147766 1105 96-97 0.7089920743733877 1105 98-99 0.8201835018935597 1105 100-101 0.9945354141864406 1106 1 0.2797995423985533 1106 2 0.26333897580886045 1106 3 0.32739902648002683 1106 4 0.27019080744198476 1106 5 0.22297579139792134 1106 6 0.3373955601744285 1106 7 0.34636462303255655 1106 8 0.2713799535169912 1106 9 0.41686372133247573 1106 10-11 0.3494458305208852 1106 12-13 0.4611264127740924 1106 14-15 0.44401963721781357 1106 16-17 0.4537338600346601 1106 18-19 0.5928491682832373 1106 20-21 0.4325649163479852 1106 22-23 0.5522757835711047 1106 24-25 0.5413683334253534 1106 26-27 0.48973293483712865 1106 28-29 0.34337253888610775 1106 30-31 0.41827250054405596 1106 32-33 0.48843178539157606 1106 34-35 0.32600198383217815 1106 36-37 0.41303225452756465 1106 38-39 0.5083932061291918 1106 40-41 0.5243942603436338 1106 42-43 0.4681391053267987 1106 44-45 0.4566890488743809 1106 46-47 0.3069123895035233 1106 48-49 0.3783749862012158 1106 50-51 0.28951391249066205 1106 52-53 0.3821305624261342 1106 54-55 0.44862482384652225 1106 56-57 0.4401912824300851 1106 58-59 0.5141939484396119 1106 60-61 0.7022915531184921 1106 62-63 0.6894954101299007 1106 64-65 0.4609978368043741 1106 66-67 0.5325170916693196 1106 68-69 0.5977634505487828 1106 70-71 0.644172609705187 1106 72-73 0.4944494787133493 1106 74-75 0.5355056846919872 1106 76-77 0.4796415983168387 1106 78-79 0.6848213232803069 1106 80-81 0.7055194711074115 1106 82-83 0.7560457551874897 1106 84-85 0.652383945259384 1106 86-87 0.714761750940955 1106 88-89 0.7906986926730184 1106 90-91 0.8115156651853859 1106 92-93 0.8202139242361604 1106 94-95 0.906360016475972 1106 96-97 0.8501467525767872 1106 98-99 0.9091273492160923 1106 100-101 0.9889444318818477 1107 1 0.16587125860181473 1107 2 0.14675665028897455 1107 3 0.22036274519593135 1107 4 0.23900728510918867 1107 5 0.2613007823061153 1107 6 0.17795823415862344 1107 7 0.14477567867444208 1107 8 0.20706219638405088 1107 9 0.22098816342590055 1107 10-11 0.2212397142118263 1107 12-13 0.4019689161628506 1107 14-15 0.48316560210690085 1107 16-17 0.3703367099040662 1107 18-19 0.337787839887028 1107 20-21 0.34587344942564613 1107 22-23 0.406565914823787 1107 24-25 0.33406965256444465 1107 26-27 0.3171337613653833 1107 28-29 0.49048687088597376 1107 30-31 0.554127957283562 1107 32-33 0.49348976800143163 1107 34-35 0.48259721641722564 1107 36-37 0.466416960448818 1107 38-39 0.649916107573965 1107 40-41 0.4667410715976459 1107 42-43 0.519698069852204 1107 44-45 0.36740357924102796 1107 46-47 0.5382103033462116 1107 48-49 0.5896053230941263 1107 50-51 0.5803480448012976 1107 52-53 0.6149095355474401 1107 54-55 0.6275511041387816 1107 56-57 0.5934958217232449 1107 58-59 0.6510337804890654 1107 60-61 0.633221033728141 1107 62-63 0.6203894492081545 1107 64-65 0.6052215416345987 1107 66-67 0.47129123786563554 1107 68-69 0.6367222365440881 1107 70-71 0.5971431257894295 1107 72-73 0.7347397025372047 1107 74-75 0.7459132490785443 1107 76-77 0.8939763488706127 1107 78-79 0.9508069218520667 1107 80-81 0.8988438206296792 1107 82-83 0.9371789055825666 1107 84-85 0.9226298539445459 1107 86-87 1.0960708394455985 1107 88-89 1.1444380519483168 1107 90-91 1.0973194539908846 1107 92-93 1.0385319855314954 1107 94-95 1.0290582014199359 1107 96-97 1.1384257620653315 1107 98-99 1.23911686222565 1107 100-101 1.2515704113178252 1108 1 0.25605147838203735 1108 2 0.3174785811188805 1108 3 0.3337767275326122 1108 4 0.34868921054572155 1108 5 0.28738963823546015 1108 6 0.2127328043736867 1108 7 0.3641009129493682 1108 8 0.3471598704890866 1108 9 0.30620841556640244 1108 10-11 0.37335362238953707 1108 12-13 0.4195321333138011 1108 14-15 0.5508577749647117 1108 16-17 0.5332026439700002 1108 18-19 0.5830355982636206 1108 20-21 0.41413308672085947 1108 22-23 0.4617408356085235 1108 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7 6143.0 8 7695.0 9 9439.0 10 11675.0 11 14894.0 12 19341.0 13 26631.0 14 23206.0 15 23599.0 16 28184.0 17 35496.0 18 31397.0 19 25860.0 20 26784.0 21 29239.0 22 31896.0 23 35304.0 24 40533.0 25 47331.0 26 54351.0 27 63543.0 28 75358.0 29 88991.0 30 106876.0 31 130612.0 32 161444.0 33 206743.0 34 277869.0 35 396138.0 36 605190.0 37 896731.0 38 773119.0 39 113713.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75907824736109 19.21136248422709 14.591226355839506 23.438332912572317 2 15.096848576808148 20.489249368769556 40.311289448954184 24.10261260546811 3 19.254593213594738 24.227821327232192 30.023854401201056 26.493731057972013 4 12.943194485295026 16.79460357384483 37.63942531413879 32.62277662672135 5 13.917163092899665 36.98094347988179 34.58780536421232 14.514088063006225 6 31.526015663075434 36.152731829911666 17.814715744602484 14.506536762410416 7 27.96772717002507 32.599102419496475 21.3650392798154 18.06813113066305 8 25.527874979224595 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22.47326896063393 32-33 22.318824921032903 26.66268929753761 28.450506238524444 22.567979542905046 34-35 22.73905500928984 26.697311143111257 28.332043531460638 22.231590316138263 36-37 22.698837159721503 26.449376639731582 28.54567243969499 22.30611376085192 38-39 22.371304584253497 26.780142378235507 28.500321454496735 22.348231583014254 40-41 22.537013380177672 26.765327671722805 28.3671605542395 22.330498393860022 42-43 22.379014930847468 26.753784142321603 28.082133533761727 22.785067393069205 44-45 22.57893355970914 26.711235719069915 28.131483705250943 22.578347015970007 46-47 22.835089726574274 26.90824973767738 27.807506233651715 22.44915430209663 48-49 22.588370480623595 26.872836440240683 27.907554700002553 22.63123837913317 50-51 22.408352157838184 27.10151131867419 27.954099229816887 22.536037293670734 52-53 22.663490676884702 27.221019469256554 27.49651206653515 22.618977787323598 54-55 22.61815613367637 27.015513070094016 27.559919378462038 22.806411417767578 56-57 22.637995243783177 27.111304224660877 27.54628334854986 22.704417183006086 58-59 22.727734492198955 26.91195407713471 27.648012494569652 22.712298936096683 60-61 22.60493941675034 26.988288865118008 27.961074161805012 22.44569755632664 62-63 22.332128328706855 26.980137048410867 28.019594521131342 22.668140101750932 64-65 22.356953429909314 27.1959393144378 27.735270267892076 22.711836987760815 66-67 22.4431832284752 26.986550539724917 27.501589682699784 23.068676549100097 68-69 22.321977644284097 26.925151387748297 27.820806283485787 22.932064684481823 70-71 22.671445560999167 26.75583182323883 27.574408355129094 22.998314260632906 72-73 22.456177522172275 26.615216131396753 27.837724804169472 23.090881542261496 74-75 22.221619248690217 27.242366740561636 27.4672219639214 23.06879204682675 76-77 22.328726594983568 26.839440315523245 27.535622637193498 23.29621045229969 78-79 22.35490631419501 26.860025675747607 27.6760155497957 23.109052460261683 80-81 22.470452238637645 26.752351415360327 27.587289951505582 23.189906394496447 82-83 22.356279433416667 26.667371967104724 27.851870666547264 23.12447793293134 84-85 22.337380685230077 26.792152170686272 27.62261274588757 23.247854398196083 86-87 22.20845067626637 26.66167913380698 27.81404510457634 23.315825085350312 88-89 22.312003398339584 26.625846285592726 27.518237537710768 23.543912778356926 90-91 22.240274849177148 26.475780631025426 27.68945941808444 23.59448510171299 92-93 22.373186811599894 26.494166974665568 27.66988790645137 23.462758307283167 94-95 22.281452647909177 26.370710105070167 27.747718778647485 23.600118468373175 96-97 22.0692372918452 26.20811125115582 28.043512989184364 23.67913846781461 98-99 22.08960844732785 26.373146573665913 27.6713121915469 23.86593278745934 100-101 22.337499412046103 25.51656755390085 28.222452653778568 23.92348038027448 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3140.0 1 1799.0 2 497.5 3 534.0 4 487.0 5 529.5 6 898.5 7 1801.5 8 2881.5 9 3439.0 10 3407.0 11 3302.0 12 3605.0 13 4179.5 14 4656.5 15 5062.0 16 5447.5 17 5766.5 18 6075.5 19 6498.5 20 7132.5 21 8132.5 22 9693.5 23 11808.5 24 14604.5 25 18194.0 26 23726.0 27 31142.5 28 37557.0 29 43834.0 30 50716.5 31 58669.5 32 67566.0 33 75477.0 34 83258.0 35 91373.0 36 100959.5 37 109710.5 38 117285.0 39 124432.5 40 129529.5 41 135353.0 42 139182.5 43 144296.0 44 152459.5 45 158438.0 46 163291.0 47 173031.5 48 189631.5 49 189018.0 50 173249.5 51 164333.5 52 160097.5 53 157246.0 54 152546.0 55 142097.0 56 129750.0 57 117021.5 58 105645.0 59 94778.5 60 79890.0 61 65698.0 62 52236.5 63 38571.0 64 27870.0 65 20568.5 66 14771.0 67 10727.0 68 7968.0 69 5876.5 70 4211.5 71 2656.5 72 1773.0 73 1166.0 74 736.0 75 515.5 76 354.0 77 213.5 78 129.0 79 83.5 80 63.0 81 62.0 82 42.0 83 32.5 84 30.5 85 29.5 86 33.0 87 28.0 88 19.0 89 11.5 90 12.0 91 13.0 92 9.5 93 8.5 94 8.5 95 7.5 96 4.0 97 2.0 98 3.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3316840039289415 2 0.08792083937129754 3 0.09860511803883332 4 0.10421660893564831 5 0.1160680777097216 6 0.05912266808884292 7 0.061277480593219885 8 0.06204064335518673 9 0.06540753789327573 10-11 0.06236610982720199 12-13 0.0609071221940301 14-15 0.0586288568899232 16-17 0.060738777467125644 18-19 0.06886421628571378 20-21 0.0706711163544882 22-23 0.08703422380960077 24-25 0.09581059557221944 26-27 0.09080514569226046 28-29 0.08845954249739178 30-31 0.08239913232883157 32-33 0.0738472202020855 34-35 0.32635308757696724 36-37 0.5080643857976308 38-39 0.4801303841132857 40-41 0.4623644039339693 42-43 0.4932051579028646 44-45 0.5027222464638629 46-47 0.49714442451242874 48-49 0.5145288233107617 50-51 0.5107915703734829 52-53 0.509972292702548 54-55 0.5012969277760719 56-57 0.47962534993257233 58-59 0.4618818157168433 60-61 0.4222197980581548 62-63 0.4930480361577538 64-65 0.5159429190167591 66-67 0.5261782784125496 68-69 0.5380970850773847 70-71 0.5354260154105008 72-73 0.5233164180551739 74-75 0.5253141088144401 76-77 0.4995012506890911 78-79 0.48007426920431756 80-81 0.4858204358826561 82-83 0.4948212672811474 84-85 0.49654960647736635 86-87 0.49584255862436766 88-89 0.5025875706823393 90-91 0.5171662240322648 92-93 0.5232154112190313 94-95 0.5293094903329725 96-97 0.5213299502777015 98-99 0.5241581416896962 100-101 0.5027559154092438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4455144.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.64357290040573 #Duplication Level Percentage of deduplicated Percentage of total 1 69.02090261681504 32.19381502859217 2 15.214515796682477 14.193187534138671 3 5.953629693092959 8.330956818354048 4 3.009761101683313 5.615440454366842 5 1.8107081971277057 4.222894989704418 6 1.106999489109217 3.09806468225866 7 0.7708697861385125 2.5169284746530316 8 0.5659908581325241 2.111986868181407 9 0.39853793134059823 1.6730309748655876 >10 1.9855854889941567 16.124874411447212 >50 0.11047940894192694 3.5138150560575334 >100 0.04864870365908042 3.9307551688433007 >500 0.0022151816747727 0.6953703974674551 >1k 9.149660644380106E-4 0.8747271376166248 >5k 1.444683259638663E-4 0.42940088564864687 >10k+ 9.63122173092442E-5 0.4747511178045614 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10944 0.24564862549897376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10192 0.22876926088135421 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8288 0.1860321462112111 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5492 0.1232732320212321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5337 0.11979410766520679 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4518 0.10141086348724082 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.244596358726003E-5 5 0.0 0.0 0.0 0.0 2.244596358726003E-5 6 0.0 0.0 0.0 0.0 2.244596358726003E-5 7 0.0 2.244596358726003E-5 0.0 0.0 2.244596358726003E-5 8 0.0 2.244596358726003E-5 0.0 0.0 6.733789076178009E-5 9 0.0 2.244596358726003E-5 0.0 2.244596358726003E-5 8.978385434904013E-5 10-11 0.0 2.244596358726003E-5 0.0 3.3668945380890044E-5 8.978385434904013E-5 12-13 0.0 4.489192717452006E-5 0.0 6.733789076178009E-5 1.2345279972993017E-4 14-15 0.0 4.489192717452006E-5 0.0 7.856087255541011E-5 1.3467578152356018E-4 16-17 0.0 4.489192717452006E-5 0.0 1.2345279972993017E-4 1.6834472690445022E-4 18-19 0.0 4.489192717452006E-5 0.0 1.9079069049171026E-4 2.0201367228534026E-4 20-21 0.0 4.489192717452006E-5 0.0 3.030205084280104E-4 2.132366540789703E-4 22-23 0.0 4.489192717452006E-5 0.0 4.6014225353883064E-4 2.805745448407504E-4 24-25 0.0 7.856087255541011E-5 0.0 6.172639986496509E-4 3.3668945380890044E-4 26-27 0.0 1.1222981793630015E-4 0.0 8.978385434904012E-4 3.3668945380890044E-4 28-29 0.0 1.3467578152356018E-4 0.0 0.0033781175198826347 3.3668945380890044E-4 30-31 2.244596358726003E-5 1.5712174511082021E-4 0.0 0.009887446960188043 3.4791243560253047E-4 32-33 2.244596358726003E-5 1.6834472690445022E-4 0.0 0.022603085332370848 4.489192717452006E-4 34-35 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.042041289798938036 4.938111989197207E-4 36-37 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.07498074136324213 4.938111989197207E-4 38-39 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.12221827173263086 4.938111989197207E-4 40-41 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.1743490221640423 4.938111989197207E-4 42-43 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.23098018829469935 4.938111989197207E-4 44-45 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.3032112991185021 4.938111989197207E-4 46-47 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.38736121660713996 4.938111989197207E-4 48-49 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.48042218163992 4.938111989197207E-4 50-51 2.244596358726003E-5 1.7956770869808025E-4 0.0 0.5816871463638437 4.938111989197207E-4 52-53 2.244596358726003E-5 2.0201367228534026E-4 0.0 0.683995848394575 5.723720714751307E-4 54-55 2.244596358726003E-5 2.0201367228534026E-4 0.0 0.802993124352434 6.060410168560208E-4 56-57 2.244596358726003E-5 2.0201367228534026E-4 0.0 0.9481062789440701 6.060410168560208E-4 58-59 2.244596358726003E-5 2.0201367228534026E-4 0.0 1.1030170966415453 6.060410168560208E-4 60-61 2.244596358726003E-5 2.0201367228534026E-4 0.0 1.275738786445511 6.284869804432809E-4 62-63 2.244596358726003E-5 2.0201367228534026E-4 0.0 1.458808065463204 6.284869804432809E-4 64-65 2.244596358726003E-5 2.132366540789703E-4 0.0 1.6586332562987862 6.284869804432809E-4 66-67 2.244596358726003E-5 2.4690559945986034E-4 0.0 1.88814323397852 6.397099622369108E-4 68-69 2.244596358726003E-5 2.4690559945986034E-4 0.0 2.150098852023638 6.509329440305409E-4 70-71 2.244596358726003E-5 2.4690559945986034E-4 0.0 2.4351401436182534 6.958248712050609E-4 72-73 2.244596358726003E-5 2.4690559945986034E-4 0.0 2.7271396839249196 7.40716798379581E-4 74-75 2.244596358726003E-5 2.4690559945986034E-4 0.0 3.0445368320305697 7.40716798379581E-4 76-77 2.244596358726003E-5 2.4690559945986034E-4 0.0 3.3864561953553016 7.40716798379581E-4 78-79 2.244596358726003E-5 2.4690559945986034E-4 0.0 3.7812021339826503 7.40716798379581E-4 80-81 2.244596358726003E-5 2.4690559945986034E-4 0.0 4.209089537846588 8.080546891413611E-4 82-83 2.244596358726003E-5 2.4690559945986034E-4 0.0 4.653710407564828 9.651764342521813E-4 84-85 2.244596358726003E-5 2.581285812534904E-4 0.0 5.111776409471838 9.651764342521813E-4 86-87 2.244596358726003E-5 2.6935156304712035E-4 0.0 5.604790776684211 9.651764342521813E-4 88-89 2.244596358726003E-5 2.6935156304712035E-4 0.0 6.137714067154732 9.876223978394414E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 12455 0.0 23.044716 2 GTACATG 13100 0.0 22.935743 1 ACATGGG 13155 0.0 20.414307 3 GTATCAA 26290 0.0 20.36568 1 GAGTACT 10970 0.0 20.218332 12-13 AGAGTAC 17685 0.0 18.684355 10-11 GGTATCA 20510 0.0 18.537333 1 GTACTTT 11885 0.0 18.523289 14-15 CATGGGG 8575 0.0 18.503468 4 CAACGCA 28670 0.0 18.353712 5 TCAACGC 28880 0.0 18.220459 4 ATCAACG 29085 0.0 18.04318 3 AGTACTT 11780 0.0 17.98416 12-13 TATCAAC 29215 0.0 17.979105 2 AACGCAG 29325 0.0 17.9353 6 ACGCAGA 31660 0.0 16.418144 7 CGCAGAG 31830 0.0 16.122236 8 GTGTAGC 2665 0.0 16.02971 1 GAGTACA 11345 0.0 15.187368 1 TTTGCGT 845 0.0 15.133851 3 >>END_MODULE