FastQCFastQC Report
Fri 27 May 2016
SRR939035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455144
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107450.241181878745109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104380.2342909679238202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86360.1938433415395776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53420.11990633748314308No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53390.1198389995923813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130850.020.6800771
TACATGG128550.020.3036232
GAGTACT120700.020.05257812-13
GTATCAA278750.019.586071
GGTATCA223600.018.9198951
AGAGTAC194150.018.5895610-11
ACATGGG132750.018.5888163
GTACTTT130200.018.37074714-15
TATAGGG11350.017.5604782
AGTACTT131650.017.51953912-13
CAACGCA313700.017.1395075
TCAACGC313250.017.118684
TATCAAC315800.017.040562
ATCAACG315250.016.9799693
AACGCAG319700.016.8920576
CATGGGG80650.016.3577424
ACGCAGA344250.015.5219897
CGCAGAG344750.015.18287858
ACTTTTT160350.014.87158816-17
TACTTTT159900.014.409471514-15
Produced by FastQC (version 0.11.5)