##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939035_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4455144 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20962734313414 33.0 31.0 34.0 31.0 34.0 2 32.448473719367996 34.0 31.0 34.0 31.0 34.0 3 32.544992934010665 34.0 31.0 34.0 31.0 34.0 4 36.087168450671854 37.0 35.0 37.0 35.0 37.0 5 36.00877076027172 37.0 35.0 37.0 35.0 37.0 6 36.043179300152815 37.0 35.0 37.0 35.0 37.0 7 35.98352847854076 37.0 35.0 37.0 35.0 37.0 8 35.950564785335786 37.0 35.0 37.0 35.0 37.0 9 37.635707173550394 39.0 37.0 39.0 35.0 39.0 10-11 37.54728926831545 39.0 37.0 39.0 35.0 39.0 12-13 37.46432113080969 39.0 37.0 39.0 34.0 39.0 14-15 38.80156612221737 40.0 38.0 41.0 34.5 41.0 16-17 38.88725717956591 40.0 38.0 41.0 35.0 41.0 18-19 38.90896568101951 40.0 38.0 41.0 35.0 41.0 20-21 38.87254620726064 40.0 38.0 41.0 34.5 41.0 22-23 38.80164165288484 40.0 38.0 41.0 34.0 41.0 24-25 38.733406933647935 40.0 38.0 41.0 34.0 41.0 26-27 38.581612737994554 40.0 38.0 41.0 34.0 41.0 28-29 38.431517140635634 40.0 38.0 41.0 34.0 41.0 30-31 38.25166145022473 40.0 38.0 41.0 34.0 41.0 32-33 38.1818103971499 40.0 38.0 41.0 33.0 41.0 34-35 38.10083377776341 40.0 38.0 41.0 33.0 41.0 36-37 37.98220989040983 40.0 37.5 41.0 33.0 41.0 38-39 37.84759976332976 40.0 37.0 41.0 33.0 41.0 40-41 37.69097923658585 40.0 37.0 41.0 33.0 41.0 42-43 37.52029934385959 40.0 37.0 41.0 32.0 41.0 44-45 37.29155062103492 40.0 37.0 41.0 31.5 41.0 46-47 37.02511377858943 40.0 36.0 41.0 31.0 41.0 48-49 36.72649829051541 39.5 36.0 41.0 30.5 41.0 50-51 36.4763705729826 39.0 36.0 41.0 30.0 41.0 52-53 36.22147600616276 39.0 35.0 40.0 30.0 41.0 54-55 35.92320753268581 39.0 35.0 40.0 29.0 41.0 56-57 35.78123973097166 38.5 35.0 40.0 28.5 41.0 58-59 35.4994863241233 38.0 35.0 40.0 28.0 41.0 60-61 35.13600357249956 38.0 34.0 40.0 28.0 41.0 62-63 34.71706223188296 37.0 34.0 40.0 26.5 41.0 64-65 34.30843357700671 37.0 33.5 39.5 26.0 41.0 66-67 33.84661966032972 36.0 33.0 39.0 26.0 40.5 68-69 33.35186943452332 35.5 32.5 39.0 25.5 40.0 70-71 32.828253587313895 35.0 32.0 38.0 24.0 40.0 72-73 32.31799881215961 35.0 31.5 37.5 22.5 39.5 74-75 31.789806794123827 35.0 31.0 37.0 21.5 39.0 76-77 29.889776851208403 32.5 28.5 35.0 19.5 37.0 78-79 30.861250612774807 34.0 30.5 36.0 20.0 38.0 80-81 30.847179462661586 34.0 31.0 35.5 20.0 37.0 82-83 30.434440166243782 34.0 30.0 35.0 18.0 37.0 84-85 29.9737970310275 34.0 30.0 35.0 14.5 36.0 86-87 29.276160097182043 34.0 29.0 35.0 7.0 36.0 88-89 28.57393229040408 33.0 28.5 35.0 2.0 35.0 90-91 27.98832776224517 33.0 27.0 35.0 2.0 35.0 92-93 27.569905147847074 33.0 27.0 35.0 2.0 35.0 94-95 26.998915859958736 32.5 25.5 34.0 2.0 35.0 96-97 26.134896649805256 32.0 24.5 34.0 2.0 35.0 98-99 24.893833173518072 32.0 12.5 34.0 2.0 35.0 100-101 19.216994220613294 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09173072653126724 1101 2 0.11943334013080431 1101 3 0.15362110286312713 1101 4 0.10617141215394099 1101 5 0.11515078946850821 1101 6 0.10702874260741879 1101 7 0.12435327587377287 1101 8 0.12314276471741437 1101 9 0.1376985725435489 1101 10-11 0.21311486486245457 1101 12-13 0.15092068479106757 1101 14-15 0.2531646424129832 1101 16-17 0.19024704771266698 1101 18-19 0.18172268281094972 1101 20-21 0.31583094926149613 1101 22-23 0.2202132622448687 1101 24-25 0.22368214374738926 1101 26-27 0.1316833376402755 1101 28-29 0.1939000077506563 1101 30-31 0.0805096918137238 1101 32-33 0.16279394232810063 1101 34-35 0.20507423657133472 1101 36-37 0.2524165140485337 1101 38-39 0.17684511377176904 1101 40-41 0.2026862838383181 1101 42-43 0.22308673170364557 1101 44-45 0.18759031369209112 1101 46-47 0.1521314171308603 1101 48-49 0.15522742623074492 1101 50-51 0.14672726361660438 1101 52-53 0.047191616761814714 1101 54-55 0.08886714885787939 1101 56-57 0.027634393906119215 1101 58-59 0.060946883478017355 1101 60-61 0.1174026750447652 1101 62-63 0.04634788256343114 1101 64-65 0.05001287021146794 1101 66-67 -0.07228520350783185 1101 68-69 0.08125773400151814 1101 70-71 0.08765409978846606 1101 72-73 0.1016508083675518 1101 74-75 0.13158369699600314 1101 76-77 -0.2196350637197675 1101 78-79 0.17178790579179903 1101 80-81 0.18750845391945958 1101 82-83 0.1258709669801874 1101 84-85 0.21997513654192602 1101 86-87 0.2617601753122756 1101 88-89 0.35381768985573103 1101 90-91 0.49153117577029093 1101 92-93 0.27145872072213706 1101 94-95 0.12699365012846542 1101 96-97 0.25978897100670295 1101 98-99 0.6149852231165411 1101 100-101 0.9678277548301857 1102 1 0.13113368479073984 1102 2 0.11947443800350754 1102 3 0.1124187854918759 1102 4 0.1016789792326449 1102 5 0.09679513146055285 1102 6 0.05102802968757203 1102 7 0.08923236998838036 1102 8 0.07767861584123636 1102 9 0.1393028211450016 1102 10-11 0.1895423419099771 1102 12-13 0.1456184627263113 1102 14-15 0.23551951830874174 1102 16-17 0.23112529434023088 1102 18-19 0.19489464048573524 1102 20-21 0.15810873652339552 1102 22-23 0.18504314627967489 1102 24-25 0.18050680041910283 1102 26-27 0.11328562738231085 1102 28-29 0.17924957050865942 1102 30-31 0.18929307667746542 1102 32-33 0.2698023047013365 1102 34-35 0.12653569481928884 1102 36-37 0.17418370110135584 1102 38-39 0.17355418222913244 1102 40-41 0.24477092302822712 1102 42-43 0.3000438106123937 1102 44-45 0.2177710345785968 1102 46-47 0.2850451286576785 1102 48-49 0.2998132616729592 1102 50-51 0.17032934244125641 1102 52-53 0.1965379756751986 1102 54-55 0.2437190122020212 1102 56-57 0.32859354906269544 1102 58-59 0.22240551319023893 1102 60-61 0.1309656291717829 1102 62-63 0.24332184440671512 1102 64-65 0.25010457394078145 1102 66-67 0.025202678584577143 1102 68-69 0.1158973056578887 1102 70-71 0.23741409179078232 1102 72-73 0.29132730309240884 1102 74-75 0.25682355885465924 1102 76-77 0.08711832482667958 1102 78-79 0.3331143542472539 1102 80-81 0.2815736407525371 1102 82-83 0.32818481896748253 1102 84-85 0.45195103517465185 1102 86-87 0.5861091498159645 1102 88-89 0.35701330296501155 1102 90-91 0.45062119609733386 1102 92-93 0.5783071592833231 1102 94-95 0.6968769440997029 1102 96-97 0.8287899133349974 1102 98-99 1.0729817059975773 1102 100-101 0.8833071164806121 1103 1 0.05586424672963375 1103 2 0.09834313264698835 1103 3 0.07458564170860171 1103 4 0.11376778936494958 1103 5 0.08989113204491872 1103 6 0.08685565031859 1103 7 0.12030465203319096 1103 8 0.1264305876779801 1103 9 0.14182256433402074 1103 10-11 0.14702702405095636 1103 12-13 0.0736799897062852 1103 14-15 0.17626725724169745 1103 16-17 0.13945385641765284 1103 18-19 0.0968848983770485 1103 20-21 0.1487301750724228 1103 22-23 0.19055882251478096 1103 24-25 0.150279101588346 1103 26-27 0.06890219144924714 1103 28-29 0.05839181650074465 1103 30-31 0.1328197372813733 1103 32-33 0.10304472413704957 1103 34-35 0.13467202571483483 1103 36-37 0.13662244207606022 1103 38-39 0.1356857268182381 1103 40-41 0.14815733471204595 1103 42-43 0.21687754018454086 1103 44-45 0.23454068906008985 1103 46-47 0.23550433347409694 1103 48-49 0.06811833664234968 1103 50-51 0.09145389278683069 1103 52-53 0.09357987778226828 1103 54-55 0.297296620835958 1103 56-57 0.31651363332363047 1103 58-59 0.2690138771584998 1103 60-61 0.2739633304586988 1103 62-63 0.25797135330618914 1103 64-65 0.26968249272605505 1103 66-67 0.24774568666956043 1103 68-69 0.24636434345026004 1103 70-71 0.3541811636477945 1103 72-73 0.31252861116510644 1103 74-75 0.2957254186516032 1103 76-77 0.17874526123360468 1103 78-79 0.41696038089426324 1103 80-81 0.37740945722710606 1103 82-83 0.3085471511353042 1103 84-85 0.4620272598291777 1103 86-87 0.49050077568268335 1103 88-89 0.440230664442371 1103 90-91 0.4824888614733567 1103 92-93 0.5149040828752263 1103 94-95 0.5819671752073603 1103 96-97 0.37229700036388635 1103 98-99 0.6939428831826362 1103 100-101 0.9891740946539436 1104 1 0.06746951666330858 1104 2 0.0453987750032141 1104 3 0.07734547230732858 1104 4 0.08409118927690429 1104 5 0.018324723996052228 1104 6 0.12195893286045845 1104 7 0.09757307958960126 1104 8 0.13748975169063726 1104 9 0.13219545754643747 1104 10-11 0.16072454643276757 1104 12-13 0.12923836512999998 1104 14-15 0.18472901506137163 1104 16-17 0.15294177287244537 1104 18-19 0.17337987198769866 1104 20-21 0.20980671203643197 1104 22-23 0.15478184783545146 1104 24-25 0.18013796769987067 1104 26-27 0.1052403839793854 1104 28-29 0.18185870545505622 1104 30-31 0.07556917179526579 1104 32-33 0.18533587265294926 1104 34-35 0.27444445620226077 1104 36-37 0.26546110296066416 1104 38-39 0.23977093133916583 1104 40-41 0.28573668964720866 1104 42-43 0.22655090562746238 1104 44-45 0.2708040140907002 1104 46-47 0.3016627471044586 1104 48-49 0.22196793348836508 1104 50-51 0.1870535756900935 1104 52-53 0.163315435160996 1104 54-55 0.30105510774187394 1104 56-57 0.1776536865360825 1104 58-59 0.27213708622178245 1104 60-61 0.27578206851335096 1104 62-63 0.3892528403385356 1104 64-65 0.369998020591531 1104 66-67 0.2611458155016422 1104 68-69 0.28507464752535583 1104 70-71 0.3581583697818971 1104 72-73 0.3642946997177887 1104 74-75 0.40119919034009754 1104 76-77 0.2954609243912252 1104 78-79 0.49517523054555923 1104 80-81 0.5152220665290379 1104 82-83 0.5119886797335376 1104 84-85 0.6009926440505673 1104 86-87 0.6617833101678734 1104 88-89 0.815341292997136 1104 90-91 0.6968226307070395 1104 92-93 0.727661481722194 1104 94-95 0.8925219468395689 1104 96-97 0.8386925674696215 1104 98-99 0.9240162464106731 1104 100-101 0.9878576263880738 1105 1 -0.0174320192952635 1105 2 0.04973986466549718 1105 3 0.017966972130992076 1105 4 0.024271904301329528 1105 5 0.002700296471211061 1105 6 -0.008010320095841905 1105 7 -0.046487294666142986 1105 8 0.045819044683959476 1105 9 0.12257354795699626 1105 10-11 0.1302428221437637 1105 12-13 0.07945853837348693 1105 14-15 0.055884201655395316 1105 16-17 0.005304461439443742 1105 18-19 -0.018468424539769046 1105 20-21 0.07819204606297347 1105 22-23 0.05710353734717444 1105 24-25 -0.028847973265534677 1105 26-27 -0.006508368460274028 1105 28-29 0.07068355205137777 1105 30-31 0.11623348011715251 1105 32-33 0.12210705207034067 1105 34-35 0.1809387380253611 1105 36-37 0.12831572287312554 1105 38-39 0.16806402760625616 1105 40-41 0.17756116558869905 1105 42-43 0.23973421478618206 1105 44-45 0.20933445532730133 1105 46-47 0.19273964775570107 1105 48-49 0.13294654317532917 1105 50-51 0.21263631747044798 1105 52-53 0.19147688602406276 1105 54-55 0.20390178858288266 1105 56-57 0.21214136018360108 1105 58-59 0.13565150658302372 1105 60-61 0.21486343455512724 1105 62-63 0.15179489302907 1105 64-65 0.14641939984560537 1105 66-67 0.2613321544096294 1105 68-69 0.22156353698368036 1105 70-71 0.36886046624071867 1105 72-73 0.3460216579228046 1105 74-75 0.33626811663014067 1105 76-77 0.05349962758930715 1105 78-79 0.10592021979342192 1105 80-81 0.18914226739743611 1105 82-83 0.17461652362154112 1105 84-85 0.11448988032313423 1105 86-87 0.3249911591942478 1105 88-89 0.466335654952708 1105 90-91 0.4404898332385514 1105 92-93 0.5969457773532554 1105 94-95 0.6792161013048563 1105 96-97 0.4879027737169004 1105 98-99 0.755602455803011 1105 100-101 0.6767205949129824 1106 1 0.026311901484987743 1106 2 0.07108705283825856 1106 3 0.05813012962777009 1106 4 0.005627879408585557 1106 5 0.06007615764902141 1106 6 0.033364321233264604 1106 7 0.12688873118467825 1106 8 0.03883821011871902 1106 9 0.02789044701107457 1106 10-11 0.11007846951017797 1106 12-13 0.13676370824774864 1106 14-15 0.06968291995044495 1106 16-17 -0.0016201699857774088 1106 18-19 2.759513875076891E-4 1106 20-21 0.10077933902506686 1106 22-23 0.06073626666623966 1106 24-25 0.15202277957717314 1106 26-27 0.06130659724405518 1106 28-29 0.030491144865976594 1106 30-31 0.05061442795248894 1106 32-33 0.16022778855235487 1106 34-35 0.01764138946316507 1106 36-37 0.06864955640347148 1106 38-39 0.19631728595322784 1106 40-41 0.318798016281832 1106 42-43 0.2551177194816887 1106 44-45 0.23970109935415707 1106 46-47 0.24515951823370585 1106 48-49 0.38678991125674855 1106 50-51 0.36698186716859027 1106 52-53 0.382224438631539 1106 54-55 0.45952536925541665 1106 56-57 0.4099985279947518 1106 58-59 0.2711476548364189 1106 60-61 0.3628179272881624 1106 62-63 0.45572723210261046 1106 64-65 0.4341412881202089 1106 66-67 0.45046012168803173 1106 68-69 0.42230281056247065 1106 70-71 0.5417950678186827 1106 72-73 0.5667667890934496 1106 74-75 0.5151468836688373 1106 76-77 0.4049024344550354 1106 78-79 0.6075907323841001 1106 80-81 0.483268197312011 1106 82-83 0.46261256622068103 1106 84-85 0.5012324890224917 1106 86-87 0.7188221702389868 1106 88-89 0.8565842898806757 1106 90-91 1.0845909665245728 1106 92-93 0.9550123466922678 1106 94-95 0.990156064378084 1106 96-97 0.8532525428603144 1106 98-99 1.0017897315542612 1106 100-101 1.162142495428725 1107 1 0.08290689602990398 1107 2 0.07185999560943657 1107 3 0.10472902538602824 1107 4 0.08620531188122982 1107 5 0.11850496050550419 1107 6 0.08826171932233251 1107 7 0.10430337726528194 1107 8 0.09796108511459067 1107 9 0.04304667407491536 1107 10-11 0.12723896807201385 1107 12-13 0.14124404679372304 1107 14-15 0.1821326306655564 1107 16-17 0.16025815183126468 1107 18-19 0.12406719018805035 1107 20-21 0.19016647824548016 1107 22-23 0.1648645174018526 1107 24-25 0.13464823003506154 1107 26-27 0.20888135176516442 1107 28-29 0.17266968819258466 1107 30-31 0.19245252098651378 1107 32-33 0.20769457864723506 1107 34-35 0.17898467640071658 1107 36-37 0.30148692870485405 1107 38-39 0.18003465296483512 1107 40-41 0.22347386512635126 1107 42-43 0.24796289420976336 1107 44-45 0.2524212765618188 1107 46-47 0.2794146840352738 1107 48-49 0.2395717937574986 1107 50-51 0.1966172293532722 1107 52-53 0.11851913232015931 1107 54-55 0.14625510774187234 1107 56-57 0.22265200106645722 1107 58-59 0.17680994200538436 1107 60-61 0.31763801523077007 1107 62-63 0.4054634968561359 1107 64-65 0.3215777677710818 1107 66-67 0.3214010282242157 1107 68-69 0.4406051083110043 1107 70-71 0.5484333459950221 1107 72-73 0.46705085032265714 1107 74-75 0.4270364065967307 1107 76-77 0.36936050302381673 1107 78-79 0.5926293014983948 1107 80-81 0.6092549331866444 1107 82-83 0.4344637646866438 1107 84-85 0.6171296292347463 1107 86-87 0.6575693914510055 1107 88-89 0.862594194995232 1107 90-91 0.9122141489889479 1107 92-93 0.9968268208549951 1107 94-95 1.157720424479912 1107 96-97 1.3037626231988604 1107 98-99 1.2950338351017194 1107 100-101 1.172422258711034 1108 1 0.07765487688340045 1108 2 0.02412799264308063 1108 3 0.08737075666088856 1108 4 0.035692374511853586 1108 5 0.029335945471459013 1108 6 0.06980001905940725 1108 7 0.0609304115806637 1108 8 0.10644524880365935 1108 9 0.071636601938458 1108 10-11 0.08553463314314769 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#Quality Count 2 6.0 3 12.0 4 29.0 5 57.0 6 155.0 7 456.0 8 1109.0 9 2091.0 10 3761.0 11 5546.0 12 8228.0 13 10931.0 14 13726.0 15 17954.0 16 22954.0 17 31509.0 18 31956.0 19 24790.0 20 24168.0 21 26711.0 22 28795.0 23 32177.0 24 38039.0 25 44864.0 26 53769.0 27 65604.0 28 79171.0 29 96572.0 30 117399.0 31 142426.0 32 177404.0 33 226202.0 34 303894.0 35 438473.0 36 691647.0 37 1015269.0 38 645513.0 39 31777.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.76789920022824 19.90469174341409 15.052020432727248 23.275388623630416 2 15.456761891422587 21.0268399854191 39.55405257383375 23.962345549324557 3 19.226943057283894 24.41712321756603 30.299492002952093 26.05644172219798 4 13.363451327274719 17.44192780300704 37.16916894268738 32.025451927030865 5 13.844647894658399 37.237898483191564 34.54988211379924 14.367571508350796 6 31.489217856931223 36.58548410556426 17.493822870820786 14.431475166683724 7 27.930747019624956 32.72850439851103 21.234487594564843 18.106260987299176 8 25.522923613692395 35.345815982603476 20.05946384673537 19.071796556968753 9 25.516414284252093 17.431086402594396 20.91380211279366 36.13869720035986 10-11 24.621965081263365 26.64866724846604 27.626435868290677 21.102931801979913 12-13 25.620586001260566 23.943793960419686 27.993158021379333 22.442462016940418 14-15 22.551235616497394 25.514757904641506 26.26438125799083 25.669625220870262 16-17 22.014402360835224 28.551502474672752 27.001121315922767 22.432973848569265 18-19 21.99387940030334 27.702660692116194 28.68126530402443 21.622194603556043 20-21 22.81473954601692 26.624908196008928 28.64058939509026 21.919762862883893 22-23 22.443943450537176 26.443455026369517 28.635179917865727 22.477421605227573 24-25 22.56804137514468 26.632505364311342 28.607718929501292 22.19173433104268 26-27 22.314553693438416 26.73071846835927 28.82337809956311 22.1313497386392 28-29 22.143268545304036 27.235853655908766 28.561602049226693 22.05927574956051 30-31 22.465772150125787 26.73927038048602 28.389621076221104 22.40533639316709 32-33 22.33676397440801 26.742222024697742 28.453895092953225 22.46711890794102 34-35 22.729396198307256 26.77760137854801 28.362781179299688 22.130221243845046 36-37 22.698053262333055 26.533424396175448 28.568687898049134 22.199834443442366 38-39 22.338158945978265 26.89679486503153 28.503941851101988 22.261104337888217 40-41 22.546894036453466 26.824258688409223 28.371390106193072 22.257457168944235 42-43 22.402142991986565 26.84850118734614 28.0448374405401 22.7045183801272 44-45 22.594509431751504 26.796082116102642 28.06755826747935 22.541850184666508 46-47 22.84759998967472 27.047127046958703 27.76250060324326 22.34277236012332 48-49 22.591330879833464 27.01631752123711 27.84955049908569 22.542801099843736 50-51 22.45069122241415 27.198863239092958 27.87738676525598 22.473058773236918 52-53 22.71861470695448 27.295526250105496 27.391550082331793 22.59430896060823 54-55 22.631142786854923 27.127675334399964 27.481580842280295 22.75960103646481 56-57 22.62965013027637 27.21351992213944 27.457462654405784 22.6993672931784 58-59 22.697297427727612 27.0407055273077 27.579794651419746 22.682202393544937 60-61 22.580451833275443 27.171016857750352 27.856639818992846 22.391891489981365 62-63 22.330927054869814 27.125968402598243 27.870040087931052 22.673064454600887 64-65 22.348930710546185 27.329539485911454 27.60187856276436 22.719651240778003 66-67 22.43828195531421 27.159587233990973 27.36034961025971 23.041781200435103 68-69 22.322613330286583 27.075711548353144 27.619603546156522 22.98207157520375 70-71 22.646093283456878 26.923600518636725 27.42301226571591 23.007293932190482 72-73 22.527610779808686 26.814946946720465 27.574855043967155 23.082587229503694 74-75 22.218855327684135 27.395399565087008 27.225169377241233 23.160575729987627 76-77 22.31306270809394 27.02195220833597 27.374853248099374 23.290131835470717 78-79 22.37737067535986 27.067537108187118 27.4260459725918 23.12904624386122 80-81 22.49467720298591 26.967858061140536 27.338038097433532 23.199426638440027 82-83 22.40780544916169 26.90594288310322 27.56187005403192 23.12438161370317 84-85 22.33950130607641 27.041129482535613 27.359503494561054 23.259865716826923 86-87 22.196345538797743 26.945307322997813 27.489732422337283 23.368614715867164 88-89 22.272631149884166 26.876787239280624 27.206155542910427 23.644426067924787 90-91 22.21851918826942 26.75894206648448 27.354878906846352 23.66765983839975 92-93 22.322589348402655 26.81495816970226 27.290015766044824 23.572436715850262 94-95 22.245634835214734 26.734231165578514 27.340895301812463 23.67923869739429 96-97 21.988772977932925 26.62166475427057 27.587750250048032 23.80181201774847 98-99 21.99524863842785 26.82085023514392 27.187190806851586 23.99671031957665 100-101 22.271920235773145 25.918985529041798 27.9476693160469 23.861424919138162 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 607.0 1 450.5 2 349.0 3 404.5 4 361.5 5 383.0 6 655.5 7 1341.0 8 2210.0 9 2631.5 10 2520.0 11 2418.5 12 2607.0 13 2885.0 14 3203.5 15 3527.0 16 3798.0 17 4216.5 18 4639.0 19 5142.0 20 6077.5 21 7405.0 22 9202.5 23 11563.5 24 14535.5 25 18152.0 26 23642.5 27 30899.5 28 37420.5 29 44383.0 30 51618.0 31 59551.5 32 68346.0 33 75711.5 34 83412.5 35 91798.0 36 101436.0 37 110114.0 38 118054.5 39 125364.0 40 130386.5 41 136267.5 42 140326.5 43 145562.5 44 153816.0 45 159881.0 46 164540.0 47 175206.5 48 191667.5 49 191367.5 50 176087.0 51 166950.5 52 162735.5 53 159016.0 54 153737.5 55 142960.5 56 130010.5 57 116749.0 58 105691.0 59 94708.0 60 79275.0 61 64843.0 62 51474.5 63 37533.5 64 26650.0 65 19408.0 66 13566.0 67 9871.0 68 7316.5 69 5299.5 70 3678.5 71 2238.0 72 1395.0 73 867.5 74 517.0 75 346.0 76 223.5 77 111.5 78 59.0 79 23.5 80 10.5 81 7.0 82 6.0 83 5.0 84 4.0 85 2.5 86 2.0 87 3.5 88 3.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39848319156462736 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.978385434904013E-4 16-17 0.0024802789763922334 18-19 5.611490896815008E-4 20-21 0.0 22-23 0.0 24-25 1.2345279972993017E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013512470079530539 36-37 0.024174302783479054 38-39 0.011469887393089875 40-41 0.006430768567749999 42-43 0.005633936860402268 44-45 0.0019303528685043624 46-47 0.0013243118516483418 48-49 0.003310779629120854 50-51 6.621559258241709E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.192776709349911E-4 60-61 0.001604886396489092 62-63 0.001402872724203752 64-65 8.080546891413611E-4 66-67 0.0028281914119947637 68-69 0.002772076503026614 70-71 5.611490896815008E-5 72-73 0.0 74-75 0.0 76-77 0.09381290481295329 78-79 0.0036699150465170152 80-81 3.254664720152704E-4 82-83 0.0 84-85 1.458987633171902E-4 86-87 0.09244370103413044 88-89 0.43841456078636293 90-91 0.3892466775484698 92-93 0.0 94-95 9.651764342521812E-4 96-97 0.0 98-99 0.0 100-101 3.1424349022164043E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4455144.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.06307366316418 #Duplication Level Percentage of deduplicated Percentage of total 1 67.60966698595945 30.466994037302896 2 15.56779156483855 14.030650761182114 3 6.3296692100242415 8.55703049624354 4 3.2275136543401612 5.817667422175956 5 1.8967464649483579 4.273661283515706 6 1.2138789427590029 3.282066972942768 7 0.8197424946950391 2.5858081496287966 8 0.587975131861539 2.119677334334817 9 0.45269388368254215 1.835980004452525 >10 2.1218307912590366 16.701963313820738 >50 0.11588466937965637 3.5959129540831345 >100 0.05292392157765488 4.170751979596925 >500 0.002537791000454332 0.7859716385468748 >1k 8.956907015494656E-4 0.8677056907482007 >5k 1.4928178359156964E-4 0.43315771211966453 >10k+ 9.952118906104643E-5 0.47500024930531937 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10745 0.241181878745109 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10438 0.2342909679238202 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8636 0.1938433415395776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5342 0.11990633748314308 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5339 0.1198389995923813 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.489192717452006E-5 2 0.0 0.0 0.0 0.0 4.489192717452006E-5 3 0.0 0.0 0.0 0.0 4.489192717452006E-5 4 0.0 0.0 0.0 0.0 4.489192717452006E-5 5 0.0 0.0 0.0 0.0 4.489192717452006E-5 6 0.0 0.0 0.0 0.0 4.489192717452006E-5 7 0.0 0.0 0.0 0.0 6.733789076178009E-5 8 0.0 0.0 0.0 0.0 1.1222981793630015E-4 9 0.0 0.0 0.0 4.489192717452006E-5 1.1222981793630015E-4 10-11 0.0 2.244596358726003E-5 0.0 5.6114908968150075E-5 1.1222981793630015E-4 12-13 0.0 4.489192717452006E-5 0.0 8.978385434904013E-5 1.1222981793630015E-4 14-15 0.0 4.489192717452006E-5 0.0 1.1222981793630015E-4 1.3467578152356018E-4 16-17 0.0 4.489192717452006E-5 0.0 1.458987633171902E-4 1.7956770869808025E-4 18-19 0.0 4.489192717452006E-5 0.0 2.132366540789703E-4 1.7956770869808025E-4 20-21 0.0 8.978385434904013E-5 0.0 3.2546647201527046E-4 1.7956770869808025E-4 22-23 0.0 8.978385434904013E-5 0.0 5.050341807133507E-4 1.9079069049171026E-4 24-25 0.0 1.2345279972993017E-4 0.0 6.621559258241709E-4 2.244596358726003E-4 26-27 0.0 1.5712174511082021E-4 0.0 9.427304706649212E-4 2.6935156304712035E-4 28-29 0.0 1.5712174511082021E-4 0.0 0.003400563483469895 2.6935156304712035E-4 30-31 0.0 1.6834472690445022E-4 0.0 0.009921115905568934 2.6935156304712035E-4 32-33 0.0 2.0201367228534026E-4 0.0 0.022984666713354272 2.6935156304712035E-4 34-35 0.0 2.0201367228534026E-4 0.0 0.04275956063373036 2.917975266343804E-4 36-37 0.0 2.0201367228534026E-4 0.0 0.0763162761966841 3.1424349022164043E-4 38-39 0.0 2.0201367228534026E-4 0.0 0.12419351652830975 3.1424349022164043E-4 40-41 0.0 2.0201367228534026E-4 0.0 0.1768966390311963 3.1424349022164043E-4 42-43 0.0 2.0201367228534026E-4 0.0 0.23430219090561383 3.1424349022164043E-4 44-45 0.0 2.0201367228534026E-4 0.0 0.307431140272907 3.2546647201527046E-4 46-47 0.0 2.0201367228534026E-4 0.0 0.3928717006678123 3.591354173961605E-4 48-49 0.0 2.0201367228534026E-4 0.0 0.4867070514443529 3.591354173961605E-4 50-51 0.0 2.0201367228534026E-4 0.0 0.5885443882397516 3.591354173961605E-4 52-53 0.0 2.0201367228534026E-4 0.0 0.6918294896865287 3.815813809834205E-4 54-55 0.0 2.0201367228534026E-4 0.0 0.8122520843321787 4.1525032636431056E-4 56-57 0.0 2.4690559945986034E-4 0.0 0.9587344427026376 4.264733081579406E-4 58-59 0.0 3.1424349022164043E-4 0.0 1.1153848225781253 4.264733081579406E-4 60-61 0.0 3.1424349022164043E-4 0.0 1.2903174397954364 4.264733081579406E-4 62-63 0.0 3.1424349022164043E-4 0.0 1.4748344834645075 4.264733081579406E-4 64-65 0.0 3.1424349022164043E-4 0.0 1.6766798110229435 4.264733081579406E-4 66-67 0.0 3.1424349022164043E-4 0.0 1.9083109322616734 4.3769628995157057E-4 68-69 0.0 3.1424349022164043E-4 0.0 2.1723091329932323 4.489192717452006E-4 70-71 0.0 3.1424349022164043E-4 0.0 2.4602010619634296 4.938111989197207E-4 72-73 0.0 3.1424349022164043E-4 0.0 2.7556123887353583 5.835950532687608E-4 74-75 0.0 3.2546647201527046E-4 0.0 3.0762642015611616 5.835950532687608E-4 76-77 0.0 3.3668945380890044E-4 0.0 3.421303553824523 5.835950532687608E-4 78-79 0.0 3.3668945380890044E-4 0.0 3.8181706360108674 5.835950532687608E-4 80-81 0.0 3.3668945380890044E-4 0.0 4.247719041180263 6.060410168560208E-4 82-83 0.0 3.3668945380890044E-4 0.0 4.694606953220816 6.284869804432809E-4 84-85 0.0 3.3668945380890044E-4 0.0 5.1542890645061075 6.284869804432809E-4 86-87 0.0 3.3668945380890044E-4 0.0 5.64915522371443 6.397099622369108E-4 88-89 0.0 3.3668945380890044E-4 0.0 6.184266995634709 6.509329440305409E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13085 0.0 20.680077 1 TACATGG 12855 0.0 20.303623 2 GAGTACT 12070 0.0 20.052578 12-13 GTATCAA 27875 0.0 19.58607 1 GGTATCA 22360 0.0 18.919895 1 AGAGTAC 19415 0.0 18.58956 10-11 ACATGGG 13275 0.0 18.588816 3 GTACTTT 13020 0.0 18.370747 14-15 TATAGGG 1135 0.0 17.560478 2 AGTACTT 13165 0.0 17.519539 12-13 CAACGCA 31370 0.0 17.139507 5 TCAACGC 31325 0.0 17.11868 4 TATCAAC 31580 0.0 17.04056 2 ATCAACG 31525 0.0 16.979969 3 AACGCAG 31970 0.0 16.892057 6 CATGGGG 8065 0.0 16.357742 4 ACGCAGA 34425 0.0 15.521989 7 CGCAGAG 34475 0.0 15.1828785 8 ACTTTTT 16035 0.0 14.871588 16-17 TACTTTT 15990 0.0 14.4094715 14-15 >>END_MODULE