FastQCFastQC Report
Fri 27 May 2016
SRR939034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470512
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108960.24373047203541787No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102660.2296381264606828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83010.18568342954900915No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56480.12633899651762484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.11915861091525981No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45530.10184515778058531No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA268500.020.9068091
TACATGG131900.020.4522652
GTACATG137300.020.4423831
GAGTACT110850.020.066712-13
AGAGTAC184850.019.01006110-11
ACATGGG135500.018.826333
CAACGCA297700.018.5370585
TCAACGC299150.018.5102834
GTACTTT119250.018.4357414-15
TATCAAC303450.018.3881472
GGTATCA209950.018.3592531
ATCAACG302150.018.3578263
AACGCAG303200.018.2303856
AGTACTT118400.018.04761512-13
CATGGGG84400.017.1591284
ACGCAGA328200.016.3803027
GTATAGG9500.015.9700671
CGCAGAG329200.015.9287448
GTACCGT3905.806214E-915.7746936
AGTACAT107500.015.3208242
Produced by FastQC (version 0.11.5)