FastQCFastQC Report
Fri 27 May 2016
SRR939034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470512
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT109560.24507260018539262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106250.23766852655803186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87380.19545859624132536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.12267051290769379No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52450.11732436911029429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA270250.021.2274361
GAGTACT118750.020.63985312-13
GTACATG134700.020.3393461
TACATGG131000.020.0310762
AGAGTAC194000.019.17715610-11
GTACTTT125200.019.10275814-15
AGTACTT127050.018.99267612-13
TGTACGC4250.018.9806212
GGTATCA210750.018.6971191
CAACGCA303550.018.6961155
TCAACGC304150.018.6748354
TATCAAC307050.018.6066362
ATCAACG306000.018.5619323
AACGCAG309700.018.3401726
ACATGGG139150.017.7666263
GTGTACG5900.016.9591481
ACGCAGA337150.016.6076937
TACACCG4901.8189894E-1216.4627845
CGCAGAG338450.016.3195768
TACTTTT149800.015.77565414-15
Produced by FastQC (version 0.11.5)