##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4470512 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18356980140082 33.0 31.0 34.0 30.0 34.0 2 32.41422884000759 34.0 31.0 34.0 31.0 34.0 3 32.517553917761546 34.0 31.0 34.0 31.0 34.0 4 36.08102919755053 37.0 35.0 37.0 35.0 37.0 5 35.99231519790127 37.0 35.0 37.0 35.0 37.0 6 36.02838310242764 37.0 35.0 37.0 35.0 37.0 7 35.97271252151879 37.0 35.0 37.0 35.0 37.0 8 35.935386819227865 37.0 35.0 37.0 35.0 37.0 9 37.624763338069556 39.0 37.0 39.0 35.0 39.0 10-11 37.54502045850677 39.0 37.0 39.0 35.0 39.0 12-13 37.476786104141986 39.0 37.0 39.0 34.0 39.0 14-15 38.759872023607144 40.0 38.0 41.0 34.0 41.0 16-17 38.74966021789003 40.0 38.0 41.0 34.5 41.0 18-19 38.83368079539883 40.0 38.0 41.0 34.5 41.0 20-21 38.82394958340342 40.0 38.0 41.0 34.0 41.0 22-23 38.76838726749867 40.0 38.0 41.0 34.0 41.0 24-25 38.71532354683312 40.0 38.0 41.0 34.0 41.0 26-27 38.56458253551271 40.0 38.0 41.0 34.0 41.0 28-29 38.38978980483667 40.0 38.0 41.0 34.0 41.0 30-31 38.23984501104124 40.0 38.0 41.0 33.5 41.0 32-33 38.10795575540341 40.0 38.0 41.0 33.0 41.0 34-35 38.10123829216877 40.0 38.0 41.0 33.0 41.0 36-37 38.010471395670116 40.0 38.0 41.0 33.0 41.0 38-39 37.894563307290085 40.0 37.0 41.0 33.0 41.0 40-41 37.75129280494046 40.0 37.0 41.0 33.0 41.0 42-43 37.6009083523319 40.0 37.0 41.0 32.5 41.0 44-45 37.37692002616255 40.0 37.0 41.0 32.0 41.0 46-47 37.11836910403104 40.0 36.5 41.0 31.0 41.0 48-49 36.865366875203556 40.0 36.0 41.0 31.0 41.0 50-51 36.61542525777808 39.0 36.0 41.0 30.0 41.0 52-53 36.399948484647844 39.0 35.5 41.0 30.0 41.0 54-55 36.15543342686475 39.0 35.0 40.0 29.5 41.0 56-57 35.87209418071129 38.5 35.0 40.0 29.0 41.0 58-59 35.57029754086333 38.0 35.0 40.0 28.0 41.0 60-61 35.16383179376322 38.0 34.0 40.0 28.0 41.0 62-63 34.84784639880175 37.0 34.0 40.0 27.0 41.0 64-65 34.59764888227568 37.0 34.0 40.0 27.0 41.0 66-67 34.14353288840294 36.5 33.5 39.0 26.0 41.0 68-69 33.7262331473442 36.0 33.0 39.0 26.0 40.0 70-71 33.269585452404556 35.0 32.5 38.5 25.5 40.0 72-73 32.75884138103197 35.0 32.0 37.5 24.5 40.0 74-75 32.29399742132445 35.0 32.0 37.0 23.5 39.0 76-77 30.31316178102195 33.0 29.5 35.0 20.5 37.5 78-79 31.30557584903027 34.0 31.0 36.0 22.0 38.0 80-81 31.30805106887086 34.0 31.0 36.0 22.0 37.0 82-83 30.99190003292688 34.0 31.0 35.0 20.5 37.0 84-85 30.593287189476285 34.0 31.0 35.0 19.5 36.5 86-87 30.03130021796161 34.0 30.0 35.0 16.5 36.0 88-89 29.465625861198895 34.0 29.5 35.0 7.5 35.5 90-91 29.00759622164083 34.0 29.0 35.0 4.5 35.0 92-93 28.65187097137867 34.0 29.0 35.0 2.0 35.0 94-95 28.25890222417477 33.0 29.0 35.0 2.0 35.0 96-97 27.644935859695714 33.0 27.5 35.0 2.0 35.0 98-99 26.746554309662965 33.0 26.0 34.5 2.0 35.0 100-101 22.355736882039462 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20453821914457393 1101 2 0.17088135828559103 1101 3 0.2084547213097423 1101 4 0.13217996666711684 1101 5 0.114416539451895 1101 6 0.18481602729733737 1101 7 0.15708196770646055 1101 8 0.2033030843955217 1101 9 0.2845033623825515 1101 10-11 0.2620057993930516 1101 12-13 0.2328138280841472 1101 14-15 0.28029411615883504 1101 16-17 0.2939102586181761 1101 18-19 0.3467247769378261 1101 20-21 0.23708509049220794 1101 22-23 0.2913789441260306 1101 24-25 0.26679899105135974 1101 26-27 0.2948587586740601 1101 28-29 0.2872845134792783 1101 30-31 0.2562578565480038 1101 32-33 0.3034081064690852 1101 34-35 0.39751256879900865 1101 36-37 0.4262303056515293 1101 38-39 0.454119624178837 1101 40-41 0.4408419985847303 1101 42-43 0.4061372986981482 1101 44-45 0.3730053083804563 1101 46-47 0.37035205491009293 1101 48-49 0.1581353709092994 1101 50-51 0.23439212451406632 1101 52-53 0.29659794100831505 1101 54-55 0.28459171920690096 1101 56-57 0.09381971942688949 1101 58-59 0.1553053935011377 1101 60-61 0.22564673165670257 1101 62-63 0.14202922525251438 1101 64-65 0.16080351670687065 1101 66-67 0.28305006197789595 1101 68-69 0.32267880464296894 1101 70-71 0.32874146585768216 1101 72-73 0.21700521498547687 1101 74-75 0.12583460546425584 1101 76-77 -0.032574092244679775 1101 78-79 0.12126498522009399 1101 80-81 0.31879434828831776 1101 82-83 0.25981227534738593 1101 84-85 0.19937115086516144 1101 86-87 0.4325379341241202 1101 88-89 0.47920647610682465 1101 90-91 0.4532040288511503 1101 92-93 0.43516160930388637 1101 94-95 0.39809835739771415 1101 96-97 0.2995619250842765 1101 98-99 0.3397075206914586 1101 100-101 0.7007760270987795 1102 1 0.18407591589502204 1102 2 0.13579980575148198 1102 3 0.16105885995460767 1102 4 0.11581497477582303 1102 5 0.11788308792524305 1102 6 0.08528956915989028 1102 7 0.18096897352987185 1102 8 0.17355099146004704 1102 9 0.1998830968404235 1102 10-11 0.24426732260087647 1102 12-13 0.25681774622673714 1102 14-15 0.2691538837176779 1102 16-17 0.262385009741827 1102 18-19 0.1880757846127068 1102 20-21 0.22553826962766976 1102 22-23 0.26625133548254354 1102 24-25 0.18029941762333834 1102 26-27 0.19620722143505276 1102 28-29 0.2350998586268247 1102 30-31 0.16172480981638415 1102 32-33 0.15919244807509614 1102 34-35 0.15345259423106228 1102 36-37 0.15076686231471825 1102 38-39 0.1471063368247556 1102 40-41 0.20752745320627497 1102 42-43 0.2459033715800345 1102 44-45 0.2639187702063239 1102 46-47 0.18991748951391685 1102 48-49 0.08004323648660261 1102 50-51 0.10210587806562899 1102 52-53 0.0350219561138303 1102 54-55 0.1045081469676461 1102 56-57 0.0972540721775701 1102 58-59 0.02098970632741981 1102 60-61 -0.033138387941434644 1102 62-63 0.03853765463926351 1102 64-65 -0.03732161619655727 1102 66-67 0.007130242137307619 1102 68-69 0.05033066042320655 1102 70-71 0.007770350630295297 1102 72-73 0.03221329585566224 1102 74-75 0.06380617245655174 1102 76-77 -0.1443642171247994 1102 78-79 -0.12543384300932203 1102 80-81 0.1505004739302045 1102 82-83 0.23425109194534954 1102 84-85 0.10159735271782111 1102 86-87 0.15044225731793404 1102 88-89 0.31521709300932343 1102 90-91 0.37214937554442784 1102 92-93 0.2777354566415049 1102 94-95 0.20869433694617 1102 96-97 0.1657830610211093 1102 98-99 0.22766034242877708 1102 100-101 0.698256562350533 1103 1 0.09112810558543316 1103 2 0.1394035842431336 1103 3 0.09037719290602553 1103 4 0.09401168684634342 1103 5 0.11926681745406853 1103 6 0.03483222295199795 1103 7 0.04912641683398533 1103 8 0.10117166934131205 1103 9 0.08015049446940026 1103 10-11 0.16407480124453855 1103 12-13 0.09499474441811628 1103 14-15 0.2083484187115019 1103 16-17 0.19234006062978892 1103 18-19 0.13480745115540316 1103 20-21 0.15913434535271165 1103 22-23 0.1558015230429035 1103 24-25 0.1801620786734759 1103 26-27 0.15750087484681075 1103 28-29 0.23240780952079376 1103 30-31 0.2134095342896032 1103 32-33 0.2372900292751865 1103 34-35 0.1436182804293864 1103 36-37 0.1027813392464978 1103 38-39 0.13560174679638948 1103 40-41 0.20229720463750311 1103 42-43 0.23961293636372005 1103 44-45 0.24022889928881597 1103 46-47 0.3386466775624939 1103 48-49 0.21498671821493787 1103 50-51 0.17522189954220835 1103 52-53 0.25463427502571534 1103 54-55 0.25054898274894555 1103 56-57 0.16936241323534773 1103 58-59 0.14244342340706595 1103 60-61 0.18362550280513545 1103 62-63 0.24900997054253793 1103 64-65 0.22024803641512136 1103 66-67 0.3041442470967439 1103 68-69 0.22123298705231065 1103 70-71 0.1849945217073241 1103 72-73 0.32477391650652976 1103 74-75 0.2753005669927475 1103 76-77 0.2146261222850221 1103 78-79 0.35367101279117463 1103 80-81 0.350316193466778 1103 82-83 0.3054004507912076 1103 84-85 0.1879479179894865 1103 86-87 0.09594765742651745 1103 88-89 0.25813579001875553 1103 90-91 0.3474950466463014 1103 92-93 0.29028149896157984 1103 94-95 0.161877793209122 1103 96-97 0.04647125896619997 1103 98-99 0.07539808835333517 1103 100-101 0.6006244959822133 1104 1 0.02886844715045811 1104 2 -0.004287576048746189 1104 3 -0.020315061652226518 1104 4 0.028355867685114333 1104 5 0.04676711911580611 1104 6 0.001050468217002276 1104 7 -0.017169350320429544 1104 8 0.034894704951135225 1104 9 0.04268835751398825 1104 10-11 0.006437685220149092 1104 12-13 0.03168221293711326 1104 14-15 0.027655843688748405 1104 16-17 0.061840238465784125 1104 18-19 0.005963607512867952 1104 20-21 0.022136233368243552 1104 22-23 -0.011046794676502714 1104 24-25 -0.008092314115501154 1104 26-27 0.03503067777312907 1104 28-29 -0.011045776482447422 1104 30-31 0.044848938757546364 1104 32-33 0.011727436944077851 1104 34-35 -0.04865835063411339 1104 36-37 0.028201673678459827 1104 38-39 0.20469702754236607 1104 40-41 0.09915333340668298 1104 42-43 0.07408536295472601 1104 44-45 0.0719799391361775 1104 46-47 0.04711660344035806 1104 48-49 0.032809160823724426 1104 50-51 0.021791561385668956 1104 52-53 -0.04284968076631657 1104 54-55 0.030799142354446474 1104 56-57 -0.0348156863294804 1104 58-59 -0.04354616280458856 1104 60-61 0.19003141382740552 1104 62-63 0.1322574488193453 1104 64-65 0.0663747705341109 1104 66-67 0.1376905966735933 1104 68-69 0.16071634486193176 1104 70-71 0.05650923860363832 1104 72-73 0.007574363151206853 1104 74-75 0.040068040020045714 1104 76-77 -0.12962273344414754 1104 78-79 0.03421526397953656 1104 80-81 0.13632877355156836 1104 82-83 0.258237341008023 1104 84-85 0.09136997202961439 1104 86-87 0.06600103983669925 1104 88-89 0.18906222782798565 1104 90-91 0.36817925907153537 1104 92-93 0.2686723540382694 1104 94-95 0.28867780362966755 1104 96-97 0.29167611808008687 1104 98-99 0.45192521343902214 1104 100-101 1.0427303488957627 1105 1 0.014621259815115195 1105 2 0.01277960909842335 1105 3 0.0184769297338363 1105 4 -0.019752757577194302 1105 5 0.06339854383882226 1105 6 0.09488428425362372 1105 7 0.06770340934856733 1105 8 0.1151071136694668 1105 9 -0.015757761814889193 1105 10-11 0.013862633904153654 1105 12-13 0.0540549592244588 1105 14-15 0.10355938417662003 1105 16-17 0.06554077636575784 1105 18-19 0.07391279402560258 1105 20-21 0.1163587063806446 1105 22-23 -0.01094198950385561 1105 24-25 0.014718922454932226 1105 26-27 0.029224214280766603 1105 28-29 0.07209775136457353 1105 30-31 0.07309866989320568 1105 32-33 0.1110026647481277 1105 34-35 0.15971031028550442 1105 36-37 0.17603948622857502 1105 38-39 0.22608488354541123 1105 40-41 0.258076086106513 1105 42-43 0.13551743946100459 1105 44-45 0.24656970021678148 1105 46-47 0.24197570096826126 1105 48-49 0.12176332724148864 1105 50-51 0.1646880616208577 1105 52-53 0.3482065733833295 1105 54-55 0.3101011002949221 1105 56-57 0.2621783000589062 1105 58-59 0.18702502966845458 1105 60-61 0.1772071253196401 1105 62-63 0.11343566022004836 1105 64-65 0.08067668886486246 1105 66-67 0.26103563307277966 1105 68-69 0.14335013310752487 1105 70-71 0.17942847053480904 1105 72-73 0.14572444158915232 1105 74-75 0.27648640299964455 1105 76-77 0.1678291198731472 1105 78-79 0.3375859867455553 1105 80-81 0.2590353173553126 1105 82-83 0.3197501892229404 1105 84-85 0.18378268238130246 1105 86-87 0.13204823727786774 1105 88-89 0.21191240503329567 1105 90-91 0.31656787686580756 1105 92-93 0.43524861420003447 1105 94-95 0.46255121760838946 1105 96-97 0.28648885082732534 1105 98-99 0.30045837458784064 1105 100-101 0.9738095467069101 1106 1 -0.036243251475610805 1106 2 -0.13540679203481432 1106 3 -0.1412780906811335 1106 4 -0.09102140185683538 1106 5 -0.04490055623617195 1106 6 -0.029638463850702124 1106 7 -0.057301381918826166 1106 8 -0.06408232887698517 1106 9 -0.139313298853466 1106 10-11 -0.02395827685327845 1106 12-13 -0.028747619727298 1106 14-15 -0.053237028622305615 1106 16-17 -0.10905311164356846 1106 18-19 -0.16727544028819352 1106 20-21 -0.13967308263693923 1106 22-23 0.032581154400816104 1106 24-25 -0.09040408812752077 1106 26-27 -0.08931184521427582 1106 28-29 -0.07104094541069372 1106 30-31 -0.07955402571548831 1106 32-33 -0.0956775077374914 1106 34-35 -0.1724256575505123 1106 36-37 -0.16529081957927616 1106 38-39 -0.019345809730147323 1106 40-41 -0.02444297480507629 1106 42-43 0.07807128914020467 1106 44-45 -0.0833068725688193 1106 46-47 -0.05114476815931113 1106 48-49 -0.16617955251853544 1106 50-51 -0.055790752644618635 1106 52-53 0.03301086052606905 1106 54-55 0.056498692162264774 1106 56-57 -0.062212375718111446 1106 58-59 0.008658956007927543 1106 60-61 0.042066303721441045 1106 62-63 0.07274634266318003 1106 64-65 -0.021870807177130303 1106 66-67 0.11300973939724912 1106 68-69 0.12512748499487003 1106 70-71 0.09148027871746223 1106 72-73 0.1408042266070666 1106 74-75 0.21707197920162713 1106 76-77 0.036632761236365496 1106 78-79 0.26884223622476 1106 80-81 0.25827694305317195 1106 82-83 0.27293231987872346 1106 84-85 0.20241706701591866 1106 86-87 0.25124163782782105 1106 88-89 0.2886924650282978 1106 90-91 0.5717632772491079 1106 92-93 0.48475865502996385 1106 94-95 0.4896861438645317 1106 96-97 0.3819572492941177 1106 98-99 0.5192372263501959 1106 100-101 0.9378886859453068 1107 1 -0.12547357650448276 1107 2 -0.10599992848094786 1107 3 -0.10229194978028744 1107 4 -0.10032349722063572 1107 5 -0.14623738184250357 1107 6 -0.024668574393515996 1107 7 -0.07419092159874907 1107 8 -0.05140249056647406 1107 9 -0.06967710020637696 1107 10-11 -0.0722442445153817 1107 12-13 -0.04944738049285036 1107 14-15 -0.03845378328325921 1107 16-17 -0.09880294665429545 1107 18-19 -0.10918890341309861 1107 20-21 -0.07481992309231345 1107 22-23 -0.07712165866633569 1107 24-25 -0.1088179224782948 1107 26-27 -0.16915613658534312 1107 28-29 -0.05353022110777772 1107 30-31 -0.11471365283973256 1107 32-33 -0.07408556746361228 1107 34-35 -0.12640787995605507 1107 36-37 -0.03723150593466329 1107 38-39 -0.024049860216869945 1107 40-41 -0.10091571032727131 1107 42-43 -0.05956709561478846 1107 44-45 -0.07118655413951558 1107 46-47 -0.18166139082883603 1107 48-49 -0.24329100277426363 1107 50-51 -0.13821258897525013 1107 52-53 -0.0991989645852911 1107 54-55 -0.26168395096593855 1107 56-57 -0.1938614435287036 1107 58-59 -0.11145798014122477 1107 60-61 -0.2262970026636424 1107 62-63 -0.21495370683360449 1107 64-65 -0.2808074512952601 1107 66-67 -0.12889815533959137 1107 68-69 -0.2109923797139217 1107 70-71 -0.2884378453544727 1107 72-73 -0.2353160505749159 1107 74-75 -0.35468061937945805 1107 76-77 -0.4835598637430074 1107 78-79 -0.2691063283685402 1107 80-81 -0.11779734336821335 1107 82-83 -0.02148506174147613 1107 84-85 -0.1043196513473923 1107 86-87 -0.1293209756023046 1107 88-89 -0.08206937295223327 1107 90-91 0.15352989991863453 1107 92-93 0.2712736690540538 1107 94-95 0.17245569509370284 1107 96-97 0.06919883484106037 1107 98-99 -0.13936325211870226 1107 100-101 0.42939359437626123 1108 1 -0.09730245785814162 1108 2 -0.11213259510010687 1108 3 -0.059394823832505494 1108 4 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173.0 7 457.0 8 1112.0 9 2114.0 10 3412.0 11 5359.0 12 7927.0 13 10748.0 14 13228.0 15 17057.0 16 22003.0 17 30089.0 18 29520.0 19 22140.0 20 21364.0 21 23259.0 22 24981.0 23 28510.0 24 33107.0 25 39516.0 26 47900.0 27 59061.0 28 72070.0 29 88492.0 30 107607.0 31 131915.0 32 163753.0 33 208904.0 34 281363.0 35 405451.0 36 649924.0 37 1039297.0 38 816507.0 39 62075.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.78405000130268 19.879753766763006 15.101393327230209 23.234802904704107 2 15.460488642016843 21.099395326530832 39.550212593098955 23.889903438353368 3 19.19462468728414 24.47196204819493 30.272326749150878 26.061086515370054 4 13.339791952241711 17.441648741799597 37.18350381343345 32.03505549252524 5 13.824285677536826 37.22156047596519 34.582143588126144 14.372010258371837 6 31.466261582565934 36.64624991499855 17.46960974492407 14.417878757511444 7 27.93541321441481 32.71043674639505 21.24946762250051 18.104682416689634 8 25.496497940280666 35.336869691883166 20.05168535505553 19.11494701278064 9 25.5266958236551 17.44035135125462 20.92225677953666 36.11069604555362 10-11 24.63407994431063 26.657203917582596 27.62436383125691 21.084352306849862 12-13 25.621282305024568 23.9467873031098 27.986358162107607 22.445572229758024 14-15 22.541317858727865 25.504958769380536 26.28633179911526 25.66739157277634 16-17 22.004410785060685 28.57155479837849 26.991675923842262 22.432358492718556 18-19 21.99621654074522 27.73559270168607 28.673572512499685 21.59461824506902 20-21 22.857180564552785 26.639622038817922 28.635310675824154 21.86788672080513 22-23 22.461510001538972 26.47486462400727 28.583191366000136 22.480434008453617 24-25 22.569294076383198 26.659765145468796 28.607998367972172 22.162942410175837 26-27 22.31541935241422 26.72033986263766 28.83643976349913 22.127801021449 28-29 22.18039007612551 27.214489078655866 28.55910016570809 22.046020679510534 30-31 22.440930703239363 26.795219429005 28.384858378637617 22.378991489118025 32-33 22.32615637761402 26.74770809249589 28.444885060145236 22.48125046974485 34-35 22.700344195924362 26.80928789896428 28.36987987032088 22.120488034790476 36-37 22.676039670584004 26.564969820236794 28.572479975231612 22.186510533947594 38-39 22.322816396256286 26.903044208540678 28.4948241564422 22.27931523876084 40-41 22.53334232580415 26.865565804809922 28.373706060064112 22.227385809321813 42-43 22.398319198028847 26.872542912010665 28.0508155155425 22.678322374417988 44-45 22.560632233884483 26.81084152267169 28.109522486032034 22.519003757411788 46-47 22.87016089020875 27.057800220198985 27.72988357907009 22.342155310522173 48-49 22.616154981200665 27.00363616760394 27.84641595739719 22.533792893798203 50-51 22.40335184433756 27.216701330993846 27.90974554489951 22.470201279769082 52-53 22.705430608395638 27.32967722712745 27.399836976167386 22.565055188309525 54-55 22.626882558418366 27.134319290497377 27.49663796898431 22.742160182099948 56-57 22.63886105215689 27.225986643140654 27.43958633820914 22.695565966493323 58-59 22.724104485758208 27.05032810618221 27.563625935688684 22.6619414723709 60-61 22.611055125754547 27.16338416008357 27.814207405800133 22.41135330836175 62-63 22.316191076100456 27.13298834674865 27.879356995350868 22.671463581800026 64-65 22.30944086368824 27.302481913664284 27.64993730010634 22.738139922541137 66-67 22.453898734078113 27.152925623384185 27.365118670740074 23.02805697179763 68-69 22.29941548435746 27.08663673607163 27.65438674174779 22.95956103782312 70-71 22.658892314795263 26.91929917647017 27.445681836890273 22.9761266718443 72-73 22.501762661636967 26.82475743270569 27.58771254836135 23.08576735729599 74-75 22.23178239986829 27.39567637890246 27.222575400759464 23.14996582046978 76-77 22.336706340118727 27.009948804102034 27.368276415966868 23.285068439812374 78-79 22.40891361587685 27.053586068163604 27.428767059858284 23.108733256101264 80-81 22.466162712458885 26.998037361268683 27.32506925381254 23.21073067245989 82-83 22.39411279960774 26.91323723099278 27.590698783495043 23.101951185904433 84-85 22.361006971908363 27.059652227753777 27.331243043302422 23.248097757035435 86-87 22.181431408110452 26.98452363457803 27.482074244259813 23.351970713051706 88-89 22.278105930587873 26.923041494636387 27.19503600194922 23.60381657282652 90-91 22.256378213318097 26.769536387128067 27.309661896087306 23.664423503466526 92-93 22.33939870869377 26.86109555236626 27.29097919880318 23.50852654013679 94-95 22.249778101479205 26.754508208455764 27.32661270118501 23.66910098888002 96-97 22.024407825312313 26.641002081985377 27.575574726945636 23.75901536575668 98-99 22.01961430815978 26.853344762300157 27.196292057822458 23.930748871717604 100-101 22.166792267633177 26.173988766822198 27.74702063994084 23.912198325603782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 696.0 1 491.5 2 334.5 3 390.0 4 373.0 5 404.5 6 681.0 7 1339.0 8 2170.0 9 2670.5 10 2558.5 11 2361.0 12 2523.5 13 2863.0 14 3233.5 15 3565.0 16 3838.0 17 4192.0 18 4726.5 19 5332.0 20 6097.5 21 7359.5 22 9209.5 23 11561.5 24 14537.0 25 18211.0 26 23904.0 27 31293.5 28 37622.0 29 44415.0 30 51603.5 31 59667.0 32 68569.5 33 75989.0 34 83798.0 35 92305.5 36 101976.5 37 110935.0 38 118508.0 39 125048.5 40 130527.0 41 136963.5 42 140632.0 43 146228.0 44 154953.5 45 160871.0 46 164932.0 47 175253.0 48 192967.0 49 193105.0 50 177426.0 51 167645.0 52 163507.0 53 159996.0 54 154519.0 55 143510.0 56 130044.0 57 117091.5 58 105827.0 59 94261.5 60 79090.5 61 64866.5 62 51225.0 63 37336.0 64 26493.0 65 19235.5 66 13464.0 67 9781.0 68 7251.5 69 5204.5 70 3643.5 71 2179.5 72 1355.0 73 849.5 74 510.5 75 332.5 76 194.0 77 104.5 78 50.0 79 26.5 80 17.0 81 8.5 82 4.5 83 4.5 84 4.5 85 2.5 86 2.5 87 2.5 88 1.5 89 1.0 90 2.5 91 2.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4059490277623682 2 0.0 3 0.0 4 0.0 5 3.8026964249285094E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010737025199798144 16-17 0.0023710930649554233 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020802986324608905 36-37 0.006665903144874681 38-39 0.011542302089783005 40-41 0.002359908663705634 42-43 6.039576674886456E-4 44-45 3.2434763624390224E-4 46-47 2.2368802499579468E-4 48-49 1.9013482124642547E-4 50-51 4.026384449924304E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.7895041999663574E-4 60-61 0.002169773842459208 62-63 0.0 64-65 2.2368802499579468E-4 66-67 0.0012190997362270808 68-69 0.0013085749462253989 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12534358480639354 78-79 6.71064074987384E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14638144355724803 88-89 0.43625875514929835 90-91 0.3349392642274531 92-93 0.0 94-95 0.0 96-97 4.3619164874179965E-4 98-99 0.0 100-101 1.1184401249789734E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4470512.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.153542911307994 #Duplication Level Percentage of deduplicated Percentage of total 1 67.81841933613082 30.622419076710628 2 15.560876218830902 14.052573841690663 3 6.140207998853602 8.317564360817782 4 3.2497125351380425 5.869441376190844 5 1.871722353235969 4.225744779744735 6 1.2078351328472134 3.272282130048122 7 0.7964657912927534 2.517427659916835 8 0.5984418636511699 2.161741629623698 9 0.43709721426812337 1.7762839038782003 >10 2.1468221087765573 16.884708527677123 >50 0.11636370459412818 3.586488084919992 >100 0.05246698097683646 4.148522637428186 >500 0.0022800422120119413 0.7065191431086089 >1k 0.0010408885780204262 0.9395932859812042 >5k 1.4869836828853387E-4 0.43568821157138726 >10k+ 9.913224552568926E-5 0.48300135069204847 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10956 0.24507260018539262 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10625 0.23766852655803186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8738 0.19545859624132536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5484 0.12267051290769379 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5245 0.11732436911029429 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.2368802499579467E-5 0.0 0.0 0.0 5 0.0 2.2368802499579467E-5 0.0 0.0 0.0 6 0.0 2.2368802499579467E-5 0.0 2.2368802499579467E-5 4.4737604999158934E-5 7 0.0 2.2368802499579467E-5 0.0 2.2368802499579467E-5 4.4737604999158934E-5 8 0.0 2.2368802499579467E-5 0.0 2.2368802499579467E-5 4.4737604999158934E-5 9 0.0 2.2368802499579467E-5 0.0 2.2368802499579467E-5 4.4737604999158934E-5 10-11 0.0 2.2368802499579467E-5 0.0 2.2368802499579467E-5 4.4737604999158934E-5 12-13 0.0 2.2368802499579467E-5 0.0 5.592200624894866E-5 4.4737604999158934E-5 14-15 0.0 2.2368802499579467E-5 0.0 7.829080874852813E-5 5.592200624894866E-5 16-17 0.0 2.2368802499579467E-5 0.0 1.9013482124642547E-4 8.947520999831787E-5 18-19 0.0 2.2368802499579467E-5 0.0 2.684256299949536E-4 1.1184401249789734E-4 20-21 0.0 4.4737604999158934E-5 0.0 3.467164387434817E-4 1.1184401249789734E-4 22-23 0.0 4.4737604999158934E-5 0.0 6.039576674886455E-4 1.342128149974768E-4 24-25 0.0 4.4737604999158934E-5 0.0 8.388300937342301E-4 1.5658161749705627E-4 26-27 0.0 4.4737604999158934E-5 0.0 0.0011855465324777117 1.5658161749705627E-4 28-29 0.0 6.71064074987384E-5 0.0 0.003444795584935238 1.5658161749705627E-4 30-31 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.010703471996048774 1.67766018746846E-4 32-33 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.024135937897046246 1.7895041999663574E-4 34-35 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.04479352700540788 2.1250362374600494E-4 36-37 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.07962175249725312 2.2368802499579468E-4 38-39 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.12991800491755753 2.2368802499579468E-4 40-41 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.18458735822652977 2.2368802499579468E-4 42-43 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.24507260018539265 2.2368802499579468E-4 44-45 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.3220324651851958 2.2368802499579468E-4 46-47 2.2368802499579467E-5 6.71064074987384E-5 0.0 0.4126932217159914 2.2368802499579468E-4 48-49 2.2368802499579467E-5 7.829080874852813E-5 0.0 0.5077718167404539 2.460568274953741E-4 50-51 4.4737604999158934E-5 8.947520999831787E-5 0.0 0.6114288475235051 2.460568274953741E-4 52-53 4.4737604999158934E-5 8.947520999831787E-5 0.0 0.718564227095241 2.7961003124474335E-4 54-55 4.4737604999158934E-5 1.342128149974768E-4 0.0 0.8444670319641241 3.1316323499411253E-4 56-57 4.4737604999158934E-5 1.5658161749705627E-4 0.0 0.9973018750425007 3.1316323499411253E-4 58-59 4.4737604999158934E-5 1.7895041999663574E-4 0.0 1.1657836954693335 3.1316323499411253E-4 60-61 4.4737604999158934E-5 1.7895041999663574E-4 0.0 1.3444992430397233 3.1316323499411253E-4 62-63 4.4737604999158934E-5 1.7895041999663574E-4 0.0 1.5298583249524886 3.1316323499411253E-4 64-65 4.4737604999158934E-5 2.013192224962152E-4 0.0 1.7309985970287072 3.1316323499411253E-4 66-67 4.4737604999158934E-5 2.013192224962152E-4 0.0 1.9649427179705592 3.243476362439023E-4 68-69 4.4737604999158934E-5 2.013192224962152E-4 0.0 2.233256503953015 3.35532037493692E-4 70-71 4.4737604999158934E-5 2.013192224962152E-4 0.0 2.526813483556246 3.5790083999327147E-4 72-73 4.4737604999158934E-5 2.013192224962152E-4 0.0 2.828892976911817 4.250072474920099E-4 74-75 4.4737604999158934E-5 2.013192224962152E-4 0.0 3.1541353652557023 4.250072474920099E-4 76-77 4.4737604999158934E-5 2.013192224962152E-4 0.0 3.5067795366615724 4.250072474920099E-4 78-79 4.4737604999158934E-5 2.013192224962152E-4 0.0 3.904910667950338 4.250072474920099E-4 80-81 4.4737604999158934E-5 2.013192224962152E-4 0.0 4.335868016907236 4.250072474920099E-4 82-83 4.4737604999158934E-5 2.013192224962152E-4 0.0 4.78402697498631 4.4737604999158935E-4 84-85 4.4737604999158934E-5 2.013192224962152E-4 0.0 5.247933570025088 4.4737604999158935E-4 86-87 4.4737604999158934E-5 2.013192224962152E-4 0.0 5.746903262981958 4.585604512413791E-4 88-89 4.4737604999158934E-5 2.013192224962152E-4 0.0 6.288675659521773 4.921136549907482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 27025 0.0 21.227436 1 GAGTACT 11875 0.0 20.639853 12-13 GTACATG 13470 0.0 20.339346 1 TACATGG 13100 0.0 20.031076 2 AGAGTAC 19400 0.0 19.177156 10-11 GTACTTT 12520 0.0 19.102758 14-15 AGTACTT 12705 0.0 18.992676 12-13 TGTACGC 425 0.0 18.980621 2 GGTATCA 21075 0.0 18.697119 1 CAACGCA 30355 0.0 18.696115 5 TCAACGC 30415 0.0 18.674835 4 TATCAAC 30705 0.0 18.606636 2 ATCAACG 30600 0.0 18.561932 3 AACGCAG 30970 0.0 18.340172 6 ACATGGG 13915 0.0 17.766626 3 GTGTACG 590 0.0 16.959148 1 ACGCAGA 33715 0.0 16.607693 7 TACACCG 490 1.8189894E-12 16.462784 5 CGCAGAG 33845 0.0 16.319576 8 TACTTTT 14980 0.0 15.775654 14-15 >>END_MODULE