##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2026483 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.901518048757378 33.0 31.0 34.0 30.0 34.0 2 31.93553067062492 33.0 31.0 34.0 30.0 34.0 3 31.970026395484197 34.0 31.0 34.0 30.0 34.0 4 35.51151724440817 37.0 35.0 37.0 33.0 37.0 5 35.574021593075294 37.0 35.0 37.0 33.0 37.0 6 35.70323807305564 37.0 35.0 37.0 33.0 37.0 7 35.64845597026967 37.0 35.0 37.0 33.0 37.0 8 35.65123072831107 37.0 35.0 37.0 33.0 37.0 9 37.22772902610089 39.0 37.0 39.0 34.0 39.0 10-11 37.119303739532974 39.0 37.0 39.0 33.0 39.0 12-13 37.02876288624183 39.0 37.0 39.0 33.0 39.0 14-15 38.14528693307568 40.0 38.0 41.0 33.0 41.0 16-17 38.10989877536599 40.0 38.0 41.0 33.0 41.0 18-19 38.11776091879379 40.0 38.0 41.0 33.0 41.0 20-21 38.23941824333093 40.0 38.0 41.0 33.5 41.0 22-23 38.16412967688355 40.0 38.0 41.0 33.0 41.0 24-25 38.060829772566564 40.0 38.0 41.0 33.0 41.0 26-27 37.908919788619 40.0 37.5 41.0 33.0 41.0 28-29 37.78152098981339 40.0 37.0 41.0 33.0 41.0 30-31 37.56313376426054 40.0 37.0 41.0 32.5 41.0 32-33 37.26700692776599 40.0 36.0 41.0 32.0 41.0 34-35 36.869815586905986 39.0 35.0 41.0 31.0 41.0 36-37 36.5565575926371 39.0 35.0 41.0 30.0 41.0 38-39 36.28190416598609 39.0 35.0 41.0 30.0 41.0 40-41 36.15083398182961 39.0 35.0 41.0 30.0 41.0 42-43 35.88153145128777 39.0 35.0 41.0 29.5 41.0 44-45 35.63998143581762 39.0 35.0 41.0 27.5 41.0 46-47 35.240187063005216 39.0 35.0 41.0 25.0 41.0 48-49 34.85852928447956 39.0 35.0 41.0 23.0 41.0 50-51 33.67271844866204 38.0 33.5 40.0 21.5 40.5 52-53 33.797764649395035 38.0 33.5 39.5 22.0 40.5 54-55 34.29377941981255 38.0 34.0 40.0 22.0 41.0 56-57 34.14870393682059 38.0 34.0 40.0 20.5 41.0 58-59 33.95046911323707 38.0 34.0 40.0 19.5 41.0 60-61 33.57505885813007 38.0 34.0 40.0 17.5 41.0 62-63 33.220942144592385 37.0 33.0 40.0 13.5 41.0 64-65 32.832188081518574 36.5 33.0 39.5 10.0 41.0 66-67 32.34751710229003 36.0 32.0 39.0 7.5 41.0 68-69 31.86137386792783 35.5 32.0 39.0 4.0 40.0 70-71 31.345897794356034 35.0 31.0 38.0 2.0 40.0 72-73 30.890447390873746 35.0 31.0 37.0 2.0 39.5 74-75 30.437223258226197 35.0 30.5 37.0 2.0 39.0 76-77 30.016708504339785 35.0 30.0 36.0 2.0 39.0 78-79 29.609187691187145 34.5 29.5 36.0 2.0 38.5 80-81 29.101289771490805 34.0 29.0 35.5 2.0 37.0 82-83 28.632523687590766 34.0 29.0 35.0 2.0 37.0 84-85 28.199386572697627 34.0 28.5 35.0 2.0 36.0 86-87 27.786798359522386 34.0 27.0 35.0 2.0 36.0 88-89 27.25890964789737 34.0 26.0 35.0 2.0 35.5 90-91 26.735708614382652 33.0 25.0 35.0 2.0 35.0 92-93 26.205936837367993 33.0 24.5 35.0 2.0 35.0 94-95 25.645020955024048 33.0 21.5 35.0 2.0 35.0 96-97 24.970732545005312 32.5 15.5 35.0 2.0 35.0 98-99 24.147035035576415 32.0 2.0 35.0 2.0 35.0 100-101 21.092440203051297 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2214382864984259 1101 2 0.26245361593899474 1101 3 0.37201885507566246 1101 4 0.33855843270566766 1101 5 0.20890973322050144 1101 6 0.4135792404706251 1101 7 0.4270894111572616 1101 8 0.34040585552416047 1101 9 0.4562411344116555 1101 10-11 0.4455519906707508 1101 12-13 0.38005854959735785 1101 14-15 0.6659367851160809 1101 16-17 0.5598103104169923 1101 18-19 0.6376753608951446 1101 20-21 0.5493634979112514 1101 22-23 0.6430155502669734 1101 24-25 0.5827121790377987 1101 26-27 0.6447591713876051 1101 28-29 0.6245888773726378 1101 30-31 0.5242575438512844 1101 32-33 0.6614265152608851 1101 34-35 0.5081819114871635 1101 36-37 0.5390452929059961 1101 38-39 0.746317764223889 1101 40-41 0.6294826411136398 1101 42-43 0.8283619795311452 1101 44-45 0.8053746979947292 1101 46-47 0.7700981976683323 1101 48-49 0.7568376607173875 1101 50-51 0.8032785008043959 1101 52-53 0.7473177912344013 1101 54-55 0.8226872361581528 1101 56-57 0.9176028899096593 1101 58-59 0.8423439049219965 1101 60-61 0.8496053749913131 1101 62-63 0.9410064242521301 1101 64-65 0.8131051405722971 1101 66-67 0.9186764553294324 1101 68-69 0.74507339725157 1101 70-71 0.7258673874348993 1101 72-73 0.7329068516296466 1101 74-75 0.42650463223432666 1101 76-77 0.3672452954811831 1101 78-79 0.4918366794871183 1101 80-81 0.42190833058242916 1101 82-83 0.37154164036870085 1101 84-85 0.4131420470559739 1101 86-87 0.47265381381302163 1101 88-89 0.5165304246272058 1101 90-91 0.462069856262346 1101 92-93 0.2773651853311456 1101 94-95 0.00461767338568464 1101 96-97 -0.05648698108412731 1101 98-99 0.10658313598419866 1101 100-101 0.6019275641311488 1102 1 0.29717330416892906 1102 2 0.2924440142916751 1102 3 0.3094620983501777 1102 4 0.18970947355894197 1102 5 0.3117072252239481 1102 6 0.27211458436577374 1102 7 0.29907805113597874 1102 8 0.37319866238645005 1102 9 0.43113195905434054 1102 10-11 0.4460495700144804 1102 12-13 0.42042075149802116 1102 14-15 0.4649531946783938 1102 16-17 0.596806874646802 1102 18-19 0.6176371278296102 1102 20-21 0.5658682090809677 1102 22-23 0.5087052782395816 1102 24-25 0.6017085466073695 1102 26-27 0.6396121678534996 1102 28-29 0.5770458290231346 1102 30-31 0.5002674520572228 1102 32-33 0.4297269080029764 1102 34-35 0.397476653139222 1102 36-37 0.30671912031927206 1102 38-39 0.40703343399663083 1102 40-41 0.5072637929642667 1102 42-43 0.5413941913226665 1102 44-45 0.6231305153783424 1102 46-47 0.7066289952413101 1102 48-49 0.5943796679244002 1102 50-51 0.6148147932960271 1102 52-53 0.5252150593922167 1102 54-55 0.6895323430776941 1102 56-57 0.47291315036880377 1102 58-59 0.42898864975532547 1102 60-61 0.5082199613974652 1102 62-63 0.6312149130338227 1102 64-65 0.5732143448502356 1102 66-67 0.45184233824717523 1102 68-69 0.554134373496165 1102 70-71 0.5911240290562993 1102 72-73 0.4492143241405948 1102 74-75 0.25531680434674797 1102 76-77 0.2791092586478747 1102 78-79 0.1957894058343861 1102 80-81 0.24256199453634153 1102 82-83 0.26451725449816266 1102 84-85 0.1412896232184373 1102 86-87 0.21710351217091173 1102 88-89 0.25452099071951295 1102 90-91 0.2869310372649707 1102 92-93 0.43191020606723285 1102 94-95 0.1620336445113324 1102 96-97 0.09406294458986508 1102 98-99 -0.0758564054503772 1102 100-101 0.24666921619289894 1103 1 0.2106393839084788 1103 2 0.2220998824164866 1103 3 0.28990550344451904 1103 4 0.2401554006057367 1103 5 0.12812972103979803 1103 6 0.26746387478006284 1103 7 0.16391286325051624 1103 8 0.14952555327761274 1103 9 0.4141557800415683 1103 10-11 0.30015404762642106 1103 12-13 0.2360035517823107 1103 14-15 0.4710534537516935 1103 16-17 0.40940058810715385 1103 18-19 0.5885767843855376 1103 20-21 0.4493522863902726 1103 22-23 0.3490972125771066 1103 24-25 0.41089189255046676 1103 26-27 0.47431767258790103 1103 28-29 0.41573429911506565 1103 30-31 0.4567912845078794 1103 32-33 0.368600512331561 1103 34-35 0.24378944402351976 1103 36-37 0.3158824052769802 1103 38-39 0.4922289999456808 1103 40-41 0.298693740003273 1103 42-43 0.33986439789349987 1103 44-45 0.4908432191406362 1103 46-47 0.5198870139037624 1103 48-49 0.5303335391214077 1103 50-51 0.36792539237436017 1103 52-53 0.36065021082399795 1103 54-55 0.3416863197381801 1103 56-57 0.4140903289918185 1103 58-59 0.4053539431042452 1103 60-61 0.6629812929883556 1103 62-63 0.557686816190845 1103 64-65 0.45551081182411934 1103 66-67 0.48701612369779923 1103 68-69 0.46895829536149236 1103 70-71 0.37187751966775906 1103 72-73 0.46059463457001826 1103 74-75 0.3628270641078508 1103 76-77 0.4171592162332196 1103 78-79 0.2418287941462829 1103 80-81 0.28921582217235553 1103 82-83 0.3077830682863656 1103 84-85 0.28762304825913176 1103 86-87 0.19120363368316262 1103 88-89 0.3071816505539786 1103 90-91 0.21372792838540278 1103 92-93 0.2854747565865239 1103 94-95 -0.07934127811057934 1103 96-97 0.051129569988219714 1103 98-99 0.21466506553570497 1103 100-101 0.4490656441911547 1104 1 0.16361023648694584 1104 2 0.2139535930933576 1104 3 0.1764305014723213 1104 4 0.3320217777944592 1104 5 0.2559134370166021 1104 6 0.1960411366135233 1104 7 0.28010255426540454 1104 8 0.28760608784885733 1104 9 0.2867862256694522 1104 10-11 0.25953315521510234 1104 12-13 0.4469797231846613 1104 14-15 0.5441188107306658 1104 16-17 0.49692160173920996 1104 18-19 0.44941751299885624 1104 20-21 0.4672508176304504 1104 22-23 0.39756148134715374 1104 24-25 0.4206458011138068 1104 26-27 0.36002462505847177 1104 28-29 0.4042636059791036 1104 30-31 0.44411463388916417 1104 32-33 0.4594066247013018 1104 34-35 0.545854224342861 1104 36-37 0.5415982576387819 1104 38-39 0.6425495238059469 1104 40-41 0.5864271677058213 1104 42-43 0.5403968080529253 1104 44-45 0.49039253020460905 1104 46-47 0.5045103962846511 1104 48-49 0.5359872200208642 1104 50-51 0.441605880723948 1104 52-53 0.34103072839544524 1104 54-55 0.43025051506467804 1104 56-57 0.451800423646219 1104 58-59 0.3105844069541277 1104 60-61 0.404784032065983 1104 62-63 0.25719576663700394 1104 64-65 0.25457840655352726 1104 66-67 0.18199721281781933 1104 68-69 0.3897867630135572 1104 70-71 0.37263195489132883 1104 72-73 0.34672338744081443 1104 74-75 0.2693594564897204 1104 76-77 0.3417206938151587 1104 78-79 0.18471018670703998 1104 80-81 0.12823917855355305 1104 82-83 0.33328136775159223 1104 84-85 0.46930124441451326 1104 86-87 0.4841631086229121 1104 88-89 0.4384935178533773 1104 90-91 0.29688371759242216 1104 92-93 0.3350641504939311 1104 94-95 0.3876934303540942 1104 96-97 0.2493346474817173 1104 98-99 0.34485680763663495 1104 100-101 0.4985760112866693 1105 1 0.2055758338185001 1105 2 0.2770438498979999 1105 3 0.2951824949079729 1105 4 0.2792864114751694 1105 5 0.26923913940374433 1105 6 0.28203859422939104 1105 7 0.32491972996442087 1105 8 0.3095456001349248 1105 9 0.3127173515022932 1105 10-11 0.39367047485067275 1105 12-13 0.28552735576219135 1105 14-15 0.45164317840677626 1105 16-17 0.5122653316947634 1105 18-19 0.5082675272391413 1105 20-21 0.462374843539898 1105 22-23 0.31264904889362555 1105 24-25 0.4276563264540414 1105 26-27 0.39842568168438675 1105 28-29 0.2886427122532993 1105 30-31 0.3735656420493427 1105 32-33 0.35122305817155564 1105 34-35 0.4994175049668428 1105 36-37 0.46670645529654564 1105 38-39 0.5734593813571038 1105 40-41 0.562955504503698 1105 42-43 0.4892327500262965 1105 44-45 0.5571440694688832 1105 46-47 0.6224778872615104 1105 48-49 0.6237126154358563 1105 50-51 0.6198568982060237 1105 52-53 0.6468969911856206 1105 54-55 0.7390894866537607 1105 56-57 0.710703984582814 1105 58-59 0.65289534795874 1105 60-61 0.6912488309393368 1105 62-63 0.6549526044769607 1105 64-65 0.6734273856299282 1105 66-67 0.7281235185266439 1105 68-69 0.7290144332146831 1105 70-71 0.6404022905093392 1105 72-73 0.6103075420628592 1105 74-75 0.4063803307587719 1105 76-77 0.4347368063304309 1105 78-79 0.6366793962727151 1105 80-81 0.6788569302047343 1105 82-83 0.7752426835153194 1105 84-85 0.7476129084079055 1105 86-87 0.8875222966642262 1105 88-89 0.8399297941684232 1105 90-91 0.9157749974749905 1105 92-93 0.9326118147767382 1105 94-95 0.8433057663639509 1105 96-97 0.848330050757987 1105 98-99 1.0547874977251048 1105 100-101 1.0112079912922916 1106 1 0.17003754874330923 1106 2 0.2304397657753654 1106 3 0.15647171226077816 1106 4 0.2330585745816478 1106 5 0.210615531730312 1106 6 0.2699706166019382 1106 7 0.17881109539500528 1106 8 0.21435452161602342 1106 9 0.19003851359521207 1106 10-11 0.14956280830368485 1106 12-13 0.17905958381302156 1106 14-15 0.27561335717319224 1106 16-17 0.2983179448787183 1106 18-19 0.3545964822303276 1106 20-21 0.3150814670797928 1106 22-23 0.3091071353229964 1106 24-25 0.3408540254414305 1106 26-27 0.29460143706489816 1106 28-29 0.2263417578933371 1106 30-31 0.12493731606090108 1106 32-33 0.27220385870704433 1106 34-35 0.04217531507558192 1106 36-37 0.1279956461717191 1106 38-39 0.24238207512839693 1106 40-41 0.2599098083377669 1106 42-43 0.21135126816825078 1106 44-45 0.17106264861531173 1106 46-47 -0.06214988481475103 1106 48-49 -0.07006966742368803 1106 50-51 -0.3048997458275622 1106 52-53 -0.20897728451861042 1106 54-55 0.055298937475789955 1106 56-57 0.07701931947727303 1106 58-59 0.0654694985826012 1106 60-61 0.22176867698776448 1106 62-63 0.29396867428472007 1106 64-65 0.11291552523698556 1106 66-67 0.14998853976700843 1106 68-69 0.05801059713455814 1106 70-71 0.09812915355535168 1106 72-73 0.09280979530981526 1106 74-75 0.20018956785330744 1106 76-77 0.07143674479639728 1106 78-79 0.24742092369348612 1106 80-81 0.1554422257957846 1106 82-83 0.34564420629721226 1106 84-85 0.2317375788937106 1106 86-87 0.26289113015483423 1106 88-89 0.3790851893047993 1106 90-91 0.3894571525004977 1106 92-93 0.4467351190164486 1106 94-95 0.30621602411845217 1106 96-97 0.4218228377199722 1106 98-99 0.3991277173624006 1106 100-101 0.39548390204358697 1107 1 0.12851048629054418 1107 2 0.17047928397865775 1107 3 0.12767480370042605 1107 4 0.15896863617567902 1107 5 0.15188085558806108 1107 6 0.2205625659263788 1107 7 0.14244106580268578 1107 8 0.18285270633221984 1107 9 0.2182912488767954 1107 10-11 0.20295048002459026 1107 12-13 0.20830413538747194 1107 14-15 0.258602150395582 1107 16-17 0.2192236201375266 1107 18-19 0.26999967498814215 1107 20-21 0.27188764872349225 1107 22-23 0.20432211723193205 1107 24-25 0.21853422041712633 1107 26-27 0.27684321474566786 1107 28-29 0.3335482698507306 1107 30-31 0.4034451486552868 1107 32-33 0.4534431631537217 1107 34-35 0.40580482213799485 1107 36-37 0.30594365471374374 1107 38-39 0.43302471442542867 1107 40-41 0.3170863573010081 1107 42-43 0.40624355583771177 1107 44-45 0.22856611340828437 1107 46-47 0.37815013639616524 1107 48-49 0.4176303175433276 1107 50-51 0.5034518982244265 1107 52-53 0.5839109833732081 1107 54-55 0.6659612329088489 1107 56-57 0.48098663613154713 1107 58-59 0.5935814283847591 1107 60-61 0.5218766364357847 1107 62-63 0.4552869636551762 1107 64-65 0.6002999205920005 1107 66-67 0.4113137278337007 1107 68-69 0.4336762698398182 1107 70-71 0.3597930984975761 1107 72-73 0.48998266840057525 1107 74-75 0.5896927896474189 1107 76-77 0.6250661283000731 1107 78-79 0.6914809917743518 1107 80-81 0.5950539218660005 1107 82-83 0.5675590475551182 1107 84-85 0.6225376526170869 1107 86-87 0.40490671297110836 1107 88-89 0.5460207678377031 1107 90-91 0.6477492309438233 1107 92-93 0.6522362114816254 1107 94-95 0.8607607719453654 1107 96-97 0.8045951972182408 1107 98-99 0.5114526500743715 1107 100-101 0.8251921077853623 1108 1 0.25394181200350374 1108 2 0.2156676467938894 1108 3 0.1787115841275302 1108 4 0.13112739024651177 1108 5 0.09498202915043663 1108 6 0.16438368585576058 1108 7 0.22953675445877053 1108 8 0.254155701801821 1108 9 0.3498571885732247 1108 10-11 0.27177533465936676 1108 12-13 0.33503972505945256 1108 14-15 0.21333778304943962 1108 16-17 0.3029169266543619 1108 18-19 0.32016476207098776 1108 20-21 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-1.775943268427035 2316 90-91 -1.9871188038116543 2316 92-93 -2.2097767486903273 2316 94-95 -2.3232693243126334 2316 96-97 -2.421046611100941 2316 98-99 -2.5955341629400657 2316 100-101 -2.5624570433594123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5408.0 3 1289.0 4 1262.0 5 1446.0 6 1640.0 7 2198.0 8 2809.0 9 3525.0 10 4522.0 11 6775.0 12 10194.0 13 14955.0 14 14089.0 15 15989.0 16 21474.0 17 30274.0 18 24698.0 19 15486.0 20 14749.0 21 15653.0 22 16298.0 23 17874.0 24 19721.0 25 22592.0 26 26056.0 27 30282.0 28 35459.0 29 41915.0 30 50339.0 31 61313.0 32 74939.0 33 95999.0 34 128920.0 35 187469.0 36 265929.0 37 377369.0 38 317312.0 39 48257.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.466961643447725 19.26287817208123 14.753426407121422 19.516733777349625 2 14.856634851646518 23.20463272790132 38.6947363222166 23.243996098235563 3 21.877375540802127 26.654710035222834 31.344469037148702 20.123445386826333 4 12.040335164789024 16.968322550911687 39.77451508740455 31.21682719689474 5 11.70559474519107 39.81680691116454 34.04070639160203 14.43689195204236 6 27.84315080836483 37.43406809075805 17.555032746860473 17.167748354016645 7 24.816870253883298 36.32520959443846 21.754816475258476 17.103103676419764 8 24.885512540913066 31.427406186939322 20.740017310750687 22.94706396139693 9 25.316488196899872 18.81168612824049 23.25217390445634 32.619651770403294 10-11 24.565654520834386 26.246625529083712 27.06109042162525 22.126629528456647 12-13 28.107580205731935 25.075838594142063 27.135058858846406 19.681522341279596 14-15 23.49419986817321 25.995033043100445 29.576599016977017 20.93416807174932 16-17 19.520824152832656 29.437122673707417 29.695448076322517 21.34660509713741 18-19 19.203852650820682 29.194604195233765 32.040294232232526 19.561248921713034 20-21 21.82291319472734 26.033705895569003 30.900124187411894 21.243256722291765 22-23 20.41518569496156 26.44683342445106 31.69733802898285 21.440642851604537 24-25 21.908925315162783 26.43413636210974 31.46550446789301 20.19143385483447 26-27 21.30867768125648 25.10014722667129 33.95276201877844 19.63841307329379 28-29 19.509725773967737 28.570529023234588 32.26311791729265 19.656627285505028 30-31 20.2138673787184 26.720236806256363 31.65762651013249 21.408269304892748 32-33 20.38126135735166 27.058742197993208 32.056544797345346 20.503451647309788 34-35 21.907770200700153 26.225838164710353 31.538199559631863 20.328192074957627 36-37 21.19143673442873 25.722599605621472 33.174509152949 19.9114545070008 38-39 20.730061980782363 26.949011170421745 32.51853164663642 19.802395202159477 40-41 20.348876112945334 27.641291950907732 31.850248846720238 20.159583089426704 42-43 19.719671602414515 28.101510026974776 30.223867554141055 21.95495081646966 44-45 20.75028610756851 26.58187238313225 30.48533165668679 22.182509852612455 46-47 21.939247175476346 27.875946807004702 30.051920214969474 20.132885802549477 48-49 21.40928028152181 27.44351636449655 30.157393939604326 20.989809414377312 50-51 19.509505801469633 28.376793526394977 31.58637143092339 20.527329241212005 52-53 20.797553663615854 29.509688869670303 28.77514825323142 20.91760921348242 54-55 19.520022888401193 27.666280565387147 30.05981925303286 22.7538772931788 56-57 21.300350392927893 27.621031309303557 29.04877838390929 22.02983991385926 58-59 22.65801734820322 27.228475836431226 28.952589838909542 21.160916976456008 60-61 20.83991474063475 27.707212759969718 31.171047885559815 20.28182461383572 62-63 20.38290521672907 27.760529050580697 30.64156505838675 21.215000674303482 64-65 19.93289660666903 29.356011248534237 29.85659631414862 20.854495830648116 66-67 20.63861956875659 27.651228933864257 28.865692790960594 22.844458706418557 68-69 19.744656281521447 28.108146450240167 29.765149950012116 22.38204731822627 70-71 21.573925152867563 27.958909596399877 28.73138835118803 21.73577689954453 72-73 20.847000125229847 26.76008069265744 30.656241591931728 21.73667759018099 74-75 19.637386416671337 29.82976821834186 28.99939045287516 21.533454912111637 76-77 20.35391538448761 27.456420043519834 29.019107920218428 23.170556651774127 78-79 20.303031730164093 27.664169039821157 29.889353040368455 22.143446189646294 80-81 21.26460217026208 27.661781201390657 29.12263653997509 21.95098008837218 82-83 20.363077809435342 27.177288064751 30.369173811930267 22.09046031388339 84-85 19.987197896131402 27.693548770958365 29.812589495729984 22.506663837180245 86-87 20.091777121912095 27.98738622376313 29.370141694257235 22.55069496006753 88-89 20.022234006973168 27.657934463727308 28.584186136918145 23.735645392381382 90-91 20.247347177223986 26.374813594473817 29.436947447403515 23.940891780898685 92-93 21.093947414166568 27.61868438952767 29.312526611197175 21.97484158510859 94-95 20.33989389878325 26.970575164617582 29.520783776116115 23.16874716048305 96-97 18.791563530176692 26.790805721849626 30.834045431799485 23.5835853161742 98-99 20.120325430668924 27.573499143472986 28.629378231814794 23.676797194043296 100-101 19.82436980519279 25.85966195402228 30.05745665958014 24.258511581204793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7037.0 1 4462.0 2 2265.0 3 2541.0 4 2123.0 5 2094.0 6 3330.5 7 5968.5 8 9035.5 9 10093.0 10 8304.0 11 6617.5 12 6468.0 13 6605.5 14 6749.5 15 6948.0 16 7348.0 17 7562.5 18 7566.0 19 7739.5 20 8071.5 21 8746.0 22 9793.5 23 11025.0 24 12335.5 25 14177.5 26 16738.0 27 19297.5 28 21416.0 29 23833.5 30 26541.5 31 29538.0 32 32713.5 33 35229.5 34 38334.5 35 40740.5 36 43252.0 37 47083.5 38 48703.0 39 48071.5 40 47790.0 41 48994.0 42 49824.0 43 51510.5 44 54515.5 45 56618.5 46 64459.5 47 93988.5 48 157744.0 49 156883.5 50 92180.0 51 62441.5 52 52594.0 53 49456.0 54 47180.5 55 43766.0 56 39910.5 57 35602.5 58 32572.0 59 28868.0 60 24503.5 61 20657.5 62 16648.5 63 12601.5 64 9056.5 65 6468.5 66 4587.5 67 3554.0 68 2652.5 69 1725.0 70 1159.5 71 802.5 72 588.0 73 466.0 74 325.5 75 238.5 76 178.5 77 98.5 78 71.0 79 59.0 80 40.5 81 26.5 82 22.0 83 14.5 84 9.0 85 8.5 86 8.0 87 6.0 88 3.5 89 3.5 90 3.5 91 4.5 92 3.5 93 2.0 94 2.0 95 0.5 96 0.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3176439180590215 2 0.08675128288764328 3 0.09592974626483419 4 0.10096309714910019 5 0.11251019623653394 6 0.054182541871804504 7 0.056403137850157145 8 0.05684725704582767 9 0.05970935852903775 10-11 0.057513435839333465 12-13 0.055712285768002995 14-15 0.054182541871804504 16-17 0.055786305633948076 18-19 0.06316361893980853 20-21 0.06503878887708409 22-23 0.08043492099366242 24-25 0.08956403779355662 26-27 0.08458003348658735 28-29 0.08176727858067401 30-31 0.0758210160164186 32-33 0.06725938485543674 34-35 0.31359749872069 36-37 0.4881610159078561 38-39 0.46018150658061285 40-41 0.4429348778154073 42-43 0.4736531221826189 44-45 0.4815732478387433 46-47 0.4756269852744879 48-49 0.49415662504940827 50-51 0.48976477966999976 52-53 0.48934533376297756 54-55 0.4806850094474022 56-57 0.45986075382818414 58-59 0.44328030385648437 60-61 0.4032108830915433 62-63 0.47308563654370656 64-65 0.49556300250236496 66-67 0.5054816645390067 68-69 0.5178676554404849 70-71 0.5152029402664616 72-73 0.5028662959422803 74-75 0.5049141789000944 76-77 0.47873581964418155 78-79 0.45899718872549145 80-81 0.46610309585621984 82-83 0.47311030983235486 84-85 0.47505949963557553 86-87 0.4757256784290813 88-89 0.48125249508631457 90-91 0.49573571552290346 92-93 0.5023234835920164 94-95 0.5080230132697882 96-97 0.5004729869433892 98-99 0.5033844350038958 100-101 0.48090706904523745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2026483.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.79546759944025 #Duplication Level Percentage of deduplicated Percentage of total 1 69.24879808473199 26.865395023964002 2 14.608652868110667 11.334990380325145 3 6.025335290746879 7.012671001438034 4 3.0928186435685725 4.799493819100372 5 1.815845375473647 3.5223285214890647 6 1.1643429963497485 2.7102738593713105 7 0.7802441682031467 2.1188956143024176 8 0.5643227422939159 1.751453173143271 9 0.42388567887747997 1.4800358808682208 >10 2.0691412042456827 14.062401492888485 >50 0.13370504348521187 3.59063025245659 >100 0.062201803549897336 4.316548919484188 >500 0.0038236072725214786 1.0384198705893224 >1k 0.004205967999773626 3.8615018234214156 >5k 0.0017843500605100233 5.0125107157397935 >10k+ 8.921750302550116E-4 6.522449651418387 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 37346 1.8428972757235071 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20886 1.030652613419407 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19638 0.9690680849530935 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15622 0.7708922305294443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15408 0.7603320629879451 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12571 0.6203358231971351 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10439 0.5151289204005166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9960 0.4914919098753851 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9423 0.46499279786704356 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8813 0.4348913857160411 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8786 0.4335590281290294 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8428 0.4158929534568018 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8295 0.40932985867633725 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7512 0.3706914886530013 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7182 0.3544071181450819 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6101 0.30106346808732176 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6058 0.2989415652635625 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5452 0.26903753942174696 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5187 0.2559606964381147 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5091 0.25122342501762907 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5085 0.2509273455538487 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4956 0.24456163708257111 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4722 0.23301453799513738 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4693 0.23158348725353237 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4173 0.20592326705923514 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3662 0.18070716606060847 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3424 0.1689626806639878 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3296 0.16264631877000696 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3251 0.16042572279165432 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3070 0.151493992300947 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3007 0.14838515793125331 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2887 0.14246356865564624 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2599 0.12825175439418934 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2580 0.12731416942555157 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2320 0.11448405932840296 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2260 0.11152326469059944 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2038 0.1005683245307264 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.869315459345082E-5 0.0 12-13 0.0 0.0 0.0 1.4803973189017624E-4 0.0 14-15 0.0 0.0 0.0 2.9607946378035243E-4 0.0 16-17 0.0 0.0 0.0 5.674856389123423E-4 0.0 18-19 0.0 0.0 0.0 9.622582572861455E-4 0.0 20-21 0.0 0.0 0.0 0.0014803973189017623 0.0 22-23 0.0 0.0 0.0 0.0024673288648362707 0.0 24-25 0.0 0.0 0.0 0.0037009932972544056 0.0 26-27 0.0 0.0 0.0 0.006242342028035764 0.0 28-29 0.0 0.0 0.0 0.016457083528457924 0.0 30-31 0.0 0.0 0.0 0.03900846935306144 0.0 32-33 0.0 0.0 0.0 0.08892253228869919 0.0 34-35 0.0 0.0 0.0 0.15568845137116866 0.0 36-37 0.0 0.0 0.0 0.25112473186303563 0.0 38-39 0.0 0.0 0.0 0.39346493407544003 0.0 40-41 0.0 0.0 0.0 0.5558151733816667 0.0 42-43 0.0 0.0 0.0 0.717597927048981 0.0 44-45 0.0 0.0 0.0 0.9117273621342987 0.0 46-47 0.0 0.0 0.0 1.1487883194677675 0.0 48-49 0.0 0.0 0.0 1.3826664225655976 0.0 50-51 0.0 0.0 0.0 1.6363818497367113 0.0 52-53 0.0 0.0 0.0 1.8867170363629993 0.0 54-55 0.0 0.0 0.0 2.157629745722022 0.0 56-57 0.0 0.0 0.0 2.5136406276292473 0.0 58-59 0.0 0.0 0.0 2.8711072335667263 0.0 60-61 0.0 0.0 0.0 3.2809058847273826 0.0 62-63 0.0 0.0 0.0 3.68917479199184 0.0 64-65 0.0 0.0 0.0 4.08806784956992 0.0 66-67 0.0 0.0 0.0 4.558365404496362 0.0 68-69 0.0 0.0 0.0 5.101301121203583 0.0 70-71 0.0 0.0 0.0 5.688081271838945 0.0 72-73 0.0 0.0 0.0 6.270074804476524 0.0 74-75 0.0 0.0 0.0 6.89342076888876 0.0 76-77 0.0 0.0 0.0 7.498730559299042 0.0 78-79 0.0 0.0 0.0 8.265527023912858 0.0 80-81 0.0 0.0 0.0 9.079942935618014 0.0 82-83 0.0 0.0 0.0 9.846418647479402 0.0 84-85 0.0 0.0 0.0 10.666213336109902 0.0 86-87 0.0 0.0 0.0 11.495383874426778 0.0 88-89 0.0 0.0 0.0 12.379970619047878 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 315 0.0 27.179977 94-95 TTTGCGT 910 0.0 26.025497 3 GAGTACT 12560 0.0 24.125025 12-13 GTACATG 13245 0.0 23.940872 1 GTCGGGA 495 0.0 23.922428 2 CTTTGCG 1010 0.0 23.917688 2 TACATGG 13365 0.0 23.355377 2 ACATGGG 13300 0.0 22.044968 3 AGTACTT 12795 0.0 21.794775 12-13 GTACTTT 13795 0.0 21.77727 14-15 CATGGGG 3405 0.0 20.309824 4 CATGGGA 9610 0.0 20.30693 4 TTGCGTT 1190 0.0 19.901852 4 ATGGGAT 3270 0.0 19.265236 5 ATGGGAG 3245 0.0 18.975756 5 ACTTTTT 16070 0.0 18.96181 16-17 TACCGTG 200 1.32246205E-5 18.937675 7 GCTTTGC 1500 0.0 18.671371 1 ATCACGC 155 9.811807E-4 18.335382 3 CGGGAGT 700 0.0 18.269901 4 >>END_MODULE