##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939032_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2035777 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90635713047156 33.0 31.0 34.0 30.0 34.0 2 31.924662180582647 33.0 31.0 34.0 30.0 34.0 3 31.961843070238046 34.0 31.0 34.0 30.0 34.0 4 35.50483574576194 37.0 35.0 37.0 33.0 37.0 5 35.5630488997567 37.0 35.0 37.0 33.0 37.0 6 35.68815101064606 37.0 35.0 37.0 33.0 37.0 7 35.623080032832675 37.0 35.0 37.0 33.0 37.0 8 35.62155334302333 37.0 35.0 37.0 33.0 37.0 9 37.201554492461604 39.0 37.0 39.0 34.0 39.0 10-11 37.09307993950222 39.0 37.0 39.0 33.0 39.0 12-13 36.98915500076875 39.0 37.0 39.0 33.0 39.0 14-15 38.10996882271486 40.0 38.0 41.0 33.0 41.0 16-17 38.078238677418994 40.0 38.0 41.0 33.0 41.0 18-19 38.113946910688156 40.0 38.0 41.0 33.0 41.0 20-21 38.01474670359278 40.0 38.0 41.0 33.0 41.0 22-23 38.07611319903899 40.0 38.0 41.0 33.0 41.0 24-25 37.98852600260244 40.0 38.0 41.0 33.0 41.0 26-27 37.86985878119263 40.0 37.5 41.0 33.0 41.0 28-29 37.74902162663199 40.0 37.0 41.0 33.0 41.0 30-31 37.529798696026134 40.0 37.0 41.0 32.5 41.0 32-33 37.250372216603296 40.0 36.0 41.0 32.0 41.0 34-35 36.85050572827967 39.0 35.0 41.0 31.0 41.0 36-37 36.54183292177876 39.0 35.0 41.0 30.0 41.0 38-39 36.296792821610616 39.0 35.0 41.0 30.0 41.0 40-41 36.15877893305603 39.0 35.0 41.0 30.0 41.0 42-43 35.8948173105404 39.0 35.0 41.0 29.5 41.0 44-45 35.64943925587134 39.0 35.0 41.0 27.5 41.0 46-47 35.24070342675057 39.0 35.0 41.0 24.5 41.0 48-49 34.9048972456217 39.0 35.0 41.0 23.5 41.0 50-51 33.666382909326515 38.0 33.5 40.0 21.5 40.5 52-53 33.8311362197333 38.0 33.5 39.5 22.0 40.5 54-55 34.337914221449594 38.0 34.0 40.0 22.0 41.0 56-57 34.182144704454366 38.0 34.0 40.0 20.5 41.0 58-59 33.99222655526612 38.0 34.0 40.0 20.0 41.0 60-61 33.65809319979546 38.0 34.0 40.0 18.0 41.0 62-63 33.3282076082007 37.0 33.0 40.0 15.5 41.0 64-65 32.91473132862784 37.0 33.0 39.5 12.0 41.0 66-67 32.460177367167425 36.0 32.5 39.0 8.0 41.0 68-69 32.01803169011144 36.0 32.0 39.0 6.5 40.5 70-71 31.525062666490484 35.0 31.5 38.5 2.0 40.0 72-73 31.069870373817956 35.0 31.0 37.0 2.0 39.5 74-75 30.618962931598105 35.0 31.0 37.0 2.0 39.0 76-77 30.212795163713906 35.0 30.5 36.0 2.0 39.0 78-79 29.758421968614442 34.5 30.0 36.0 2.0 38.5 80-81 29.256230422094365 34.0 29.0 35.5 2.0 37.0 82-83 28.880175480909745 34.0 29.0 35.0 2.0 37.0 84-85 28.50456263136876 34.0 29.0 35.0 2.0 36.0 86-87 28.144166821808085 34.0 29.0 35.0 2.0 36.0 88-89 27.670795966355843 34.0 27.0 35.0 2.0 36.0 90-91 27.17150650586975 34.0 26.0 35.0 2.0 35.0 92-93 26.716807636592808 33.5 25.0 35.0 2.0 35.0 94-95 26.209090926953195 33.0 24.5 35.0 2.0 35.0 96-97 25.58823117659744 33.0 21.5 35.0 2.0 35.0 98-99 24.89279277641903 33.0 10.0 35.0 2.0 35.0 100-101 22.35627060331264 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33373653892023825 1101 2 0.31938352982254514 1101 3 0.36967992272889205 1101 4 0.3216562090714703 1101 5 0.016723870019994536 1101 6 0.19999989331137868 1101 7 0.27606554143713424 1101 8 0.26375100431764054 1101 9 0.19592264481431698 1101 10-11 0.3824300060695762 1101 12-13 0.4398176872122548 1101 14-15 0.15946150152796434 1101 16-17 0.36993679956614045 1101 18-19 0.4487521228069795 1101 20-21 0.5725825311587585 1101 22-23 0.5279929121199203 1101 24-25 0.6078947000324959 1101 26-27 0.48805904666826194 1101 28-29 0.45865835976759683 1101 30-31 0.44567867201553923 1101 32-33 0.47735288446823887 1101 34-35 0.47728532277446334 1101 36-37 0.6249954550044379 1101 38-39 0.5709497137438291 1101 40-41 0.5228982639357582 1101 42-43 0.40052408031542797 1101 44-45 0.6220097153660262 1101 46-47 0.681499705702052 1101 48-49 0.710196153203178 1101 50-51 0.5720094501625681 1101 52-53 0.4907293799940007 1101 54-55 0.39699189106251964 1101 56-57 0.4062875003451154 1101 58-59 0.32027231658843647 1101 60-61 0.33101840645591807 1101 62-63 0.37579300225486634 1101 64-65 0.33183868788104576 1101 66-67 0.26215440872331186 1101 68-69 0.23101357373401044 1101 70-71 0.2306673804735091 1101 72-73 0.22552074022956603 1101 74-75 0.22735202832524237 1101 76-77 0.20945053882633502 1101 78-79 0.21552599160473207 1101 80-81 0.013114769214208621 1101 82-83 0.04525197127198055 1101 84-85 -0.1243294517652842 1101 86-87 -0.20126283713020854 1101 88-89 -0.24110540972432304 1101 90-91 -0.23566002490211702 1101 92-93 -0.10019060603987029 1101 94-95 0.013328413293454133 1101 96-97 -0.228650876767599 1101 98-99 -0.23008420713167155 1101 100-101 0.1867077244872739 1102 1 0.30818686731354106 1102 2 0.24554383335348717 1102 3 0.2146927987713383 1102 4 0.21110213608813666 1102 5 0.2564929631807047 1102 6 0.24922997214690668 1102 7 0.29784081710010923 1102 8 0.20164238067788887 1102 9 0.37140605943734073 1102 10-11 0.35737580212930453 1102 12-13 0.31277485622900514 1102 14-15 0.4776647667210554 1102 16-17 0.48695906113413656 1102 18-19 0.49099435589253915 1102 20-21 0.5726025500543699 1102 22-23 0.5480323827091027 1102 24-25 0.5677438854580785 1102 26-27 0.5986653912123572 1102 28-29 0.647456703315207 1102 30-31 0.6191528290115045 1102 32-33 0.5367125244840025 1102 34-35 0.4185717092025385 1102 36-37 0.4918524216635234 1102 38-39 0.47484956143340185 1102 40-41 0.46159818124660035 1102 42-43 0.5034730582673959 1102 44-45 0.5495528519154647 1102 46-47 0.5350926247962349 1102 48-49 0.637865798051287 1102 50-51 0.6429697621459383 1102 52-53 0.6077754140005496 1102 54-55 0.7081959108567588 1102 56-57 0.6174572377806129 1102 58-59 0.5686040752873396 1102 60-61 0.6242476823112995 1102 62-63 0.6037081288104318 1102 64-65 0.6411372980692249 1102 66-67 0.567063458987974 1102 68-69 0.48718064476382494 1102 70-71 0.6418745421221992 1102 72-73 0.6047276972406586 1102 74-75 0.5571460950582328 1102 76-77 0.4109557373442705 1102 78-79 0.47392572557585666 1102 80-81 0.4128695933509938 1102 82-83 0.3791115420557354 1102 84-85 0.24151572654468367 1102 86-87 0.3316319619051029 1102 88-89 0.3447643918272547 1102 90-91 0.2630540390949534 1102 92-93 0.2719445370757718 1102 94-95 0.29193708336593005 1102 96-97 0.2814202959671057 1102 98-99 0.37524298781511334 1102 100-101 0.7381920143808998 1103 1 0.16484790777849057 1103 2 0.31083645046587804 1103 3 0.23101288048781754 1103 4 0.21704051006121716 1103 5 0.30127616323314044 1103 6 0.2057533618514995 1103 7 0.27683105045092304 1103 8 0.1608988480786877 1103 9 0.29376474891471105 1103 10-11 0.27750777206463084 1103 12-13 0.2990264403052123 1103 14-15 0.27179194072909496 1103 16-17 0.3679896119556716 1103 18-19 0.460232824910193 1103 20-21 0.4284182946794388 1103 22-23 0.4637647175281927 1103 24-25 0.5766620979646291 1103 26-27 0.45582923931583963 1103 28-29 0.4397299619238382 1103 30-31 0.44093455969670003 1103 32-33 0.32376311113844025 1103 34-35 0.243483902219495 1103 36-37 0.2960215463794853 1103 38-39 0.3672061206013808 1103 40-41 0.42553777878198673 1103 42-43 0.3533268043345146 1103 44-45 0.41820371059402106 1103 46-47 0.24076948963629974 1103 48-49 0.08454497345768175 1103 50-51 0.08701254312828155 1103 52-53 -0.045889400935656965 1103 54-55 0.12435662904414357 1103 56-57 0.19601526413090653 1103 58-59 0.03916702319854437 1103 60-61 0.1034117543703772 1103 62-63 0.0826273518483589 1103 64-65 -0.051846746459709436 1103 66-67 0.16467655656707336 1103 68-69 0.18836378582308555 1103 70-71 0.2636745716187896 1103 72-73 0.2605286935699702 1103 74-75 0.21496829069710444 1103 76-77 0.10287053043114369 1103 78-79 0.2133092741250593 1103 80-81 0.27604391820678487 1103 82-83 0.21332704196833774 1103 84-85 0.18078388333545803 1103 86-87 0.2346705205049986 1103 88-89 0.322873414506855 1103 90-91 0.31069717949873166 1103 92-93 0.3100772116518904 1103 94-95 0.279683297970017 1103 96-97 0.3361307377605023 1103 98-99 0.3038246387502639 1103 100-101 0.6775221686371502 1104 1 0.1742726138642432 1104 2 0.16293923400668575 1104 3 0.14088278021119294 1104 4 0.13874478046232497 1104 5 0.1570139744364596 1104 6 0.07176520486671478 1104 7 0.009494432193662306 1104 8 0.14296143452405374 1104 9 0.19400031253849903 1104 10-11 0.11502506113793487 1104 12-13 0.2878252819373017 1104 14-15 0.30838642551470485 1104 16-17 0.2710378916928775 1104 18-19 0.29191181004158295 1104 20-21 0.22874246480392202 1104 22-23 0.2872929851630417 1104 24-25 0.34796980024679414 1104 26-27 0.2649535477529028 1104 28-29 0.26004678419216276 1104 30-31 0.3477172792679326 1104 32-33 0.25867947524631774 1104 34-35 0.197236711693904 1104 36-37 0.13080067846799892 1104 38-39 0.1700026282085645 1104 40-41 0.2742994489064756 1104 42-43 0.31061090703160943 1104 44-45 0.21285490354007663 1104 46-47 0.32366951556991097 1104 48-49 0.3614575771921196 1104 50-51 0.21445894700103452 1104 52-53 0.1317633912496845 1104 54-55 0.1632948638297549 1104 56-57 0.23481518633007425 1104 58-59 0.24020868986521293 1104 60-61 0.20206428721962055 1104 62-63 0.17365000053439417 1104 64-65 0.15142629123046447 1104 66-67 0.19520581832364314 1104 68-69 0.13118017586457853 1104 70-71 0.12814876767687977 1104 72-73 0.22359923298905926 1104 74-75 0.045471618843716755 1104 76-77 -0.05453243464492985 1104 78-79 0.04749517488577837 1104 80-81 0.014237642273176476 1104 82-83 -0.07231006038297494 1104 84-85 -0.0536170502745037 1104 86-87 0.07413174975810932 1104 88-89 0.10122209189274756 1104 90-91 0.12045794194686721 1104 92-93 0.31724893537250054 1104 94-95 0.41172751477499503 1104 96-97 0.440130997096599 1104 98-99 0.37920932063131474 1104 100-101 0.8437933477519515 1105 1 0.01269272628952578 1105 2 0.13406761730098538 1105 3 0.09707660164328757 1105 4 0.06549327450454001 1105 5 0.05183752629529437 1105 6 0.12437256136303887 1105 7 0.1354670408501164 1105 8 0.13080684033067058 1105 9 0.1997523583976104 1105 10-11 0.024303291687338913 1105 12-13 0.1412981579235435 1105 14-15 0.11898091453741699 1105 16-17 0.20253660012309638 1105 18-19 0.2181691511271353 1105 20-21 0.24306426486639765 1105 22-23 0.23489202298549827 1105 24-25 0.20891284013239186 1105 26-27 0.1520462891726666 1105 28-29 0.3324901417284494 1105 30-31 0.2955863987989389 1105 32-33 0.11217305618644247 1105 34-35 0.24950269853918883 1105 36-37 0.3693095601069487 1105 38-39 0.27039007293420525 1105 40-41 0.20395096094469523 1105 42-43 0.33241859627489134 1105 44-45 0.16088704182190838 1105 46-47 0.18177781138970772 1105 48-49 0.15335260989022004 1105 50-51 0.16238415336914613 1105 52-53 0.04489890228988003 1105 54-55 -0.04085925755683917 1105 56-57 -0.0013338547422634406 1105 58-59 0.06347801780862028 1105 60-61 0.22344857326039147 1105 62-63 0.05489922737471886 1105 64-65 0.02405740862703709 1105 66-67 -0.08388555919005114 1105 68-69 -0.09380208077620722 1105 70-71 -0.08721800354543419 1105 72-73 -0.002438714211734805 1105 74-75 -0.035195012528429714 1105 76-77 -0.0561682570456945 1105 78-79 -0.16299728326924523 1105 80-81 -0.2774861159013504 1105 82-83 -0.19583109766447038 1105 84-85 -0.06656799892831344 1105 86-87 -0.1315565038760056 1105 88-89 0.07838243105561915 1105 90-91 0.028288529296609966 1105 92-93 0.11943786833877112 1105 94-95 0.07963237262893585 1105 96-97 0.12379101441537799 1105 98-99 0.1265986618258914 1105 100-101 0.3320622878050763 1106 1 0.21060446488340645 1106 2 0.13324473399841708 1106 3 0.09880214011597133 1106 4 0.08880553330158847 1106 5 0.1909611731933012 1106 6 0.14667924166558066 1106 7 0.2580686002517183 1106 8 0.12383875511720532 1106 9 0.17035629937951313 1106 10-11 0.1806784227950473 1106 12-13 0.27459707443536274 1106 14-15 0.26038548322578237 1106 16-17 0.3704039130844947 1106 18-19 0.38540609018243543 1106 20-21 0.33219070503800197 1106 22-23 0.3058401657353613 1106 24-25 0.25390002127140576 1106 26-27 0.22420988340614656 1106 28-29 0.23493871026381186 1106 30-31 0.11028113174184995 1106 32-33 0.19064869380775207 1106 34-35 0.07375759909864144 1106 36-37 0.06295174226261935 1106 38-39 0.08917242206219811 1106 40-41 0.12177159352302169 1106 42-43 0.0871820085177859 1106 44-45 0.14502766477728812 1106 46-47 0.16663789103072446 1106 48-49 0.04110000333815833 1106 50-51 0.13302017925046528 1106 52-53 -0.08888720140046047 1106 54-55 -0.14093117806077515 1106 56-57 -0.029398704992601665 1106 58-59 0.16451147870984073 1106 60-61 0.15680962123624909 1106 62-63 0.01784066526137451 1106 64-65 -0.15169008145306861 1106 66-67 0.056093181733075426 1106 68-69 0.13719346709798685 1106 70-71 0.05638809253512633 1106 72-73 -0.12627409527464906 1106 74-75 -0.09961608015283119 1106 76-77 -0.07327592541215111 1106 78-79 -0.04021931983244187 1106 80-81 0.018131816613159657 1106 82-83 -0.029597094817848557 1106 84-85 0.07113497895298693 1106 86-87 0.17405983823407922 1106 88-89 0.305303070881358 1106 90-91 0.1993457103752263 1106 92-93 0.22297458050979557 1106 94-95 0.30751883083854636 1106 96-97 0.5032808871628909 1106 98-99 0.4483756015656688 1106 100-101 0.7678006380926625 1107 1 0.049821569744516125 1107 2 -0.06773413134180473 1107 3 0.029945700461432523 1107 4 0.03041440705940346 1107 5 -0.03475102009194586 1107 6 -0.023108948473421265 1107 7 0.011249345849236647 1107 8 -0.02520435990415848 1107 9 0.035543004316323845 1107 10-11 0.04359304338246517 1107 12-13 0.12694707571672836 1107 14-15 0.033789147145064646 1107 16-17 0.0886591701364452 1107 18-19 -0.02314882918018668 1107 20-21 0.014917861725457726 1107 22-23 -0.042821594940882335 1107 24-25 -0.04684073169890013 1107 26-27 -0.04292395629689594 1107 28-29 0.058059635765168593 1107 30-31 0.03998734400314419 1107 32-33 0.22362046826361848 1107 34-35 -0.013667440968688993 1107 36-37 0.09985447768411859 1107 38-39 -0.06627616951902837 1107 40-41 -0.06270056238574995 1107 42-43 0.04444622232066564 1107 44-45 0.3739086524544817 1107 46-47 0.28523734280305035 1107 48-49 0.1464349722206677 1107 50-51 0.24593036433890347 1107 52-53 0.2352197601644619 1107 54-55 0.09116542316421317 1107 56-57 0.1849706853332833 1107 58-59 0.07704688877664267 1107 60-61 -0.10960714613266731 1107 62-63 -0.1149444978959906 1107 64-65 0.014206697586139683 1107 66-67 -0.0015219786052824702 1107 68-69 0.0398760322357532 1107 70-71 -0.06089672662465162 1107 72-73 0.014814938003421929 1107 74-75 -0.05926154086317936 1107 76-77 0.19256841617252718 1107 78-79 0.1427595514217792 1107 80-81 -0.010603317072778395 1107 82-83 0.06862505862676116 1107 84-85 0.18920019951556455 1107 86-87 0.24574834398347178 1107 88-89 0.32137547103827657 1107 90-91 0.3766356845704806 1107 92-93 0.18059523593855076 1107 94-95 0.23788506998882042 1107 96-97 0.2779765133666352 1107 98-99 0.06659729667538627 1107 100-101 0.31932413342356014 1108 1 0.10975062299370464 1108 2 0.13720187839381026 1108 3 0.07572477988721715 1108 4 0.140937844896051 1108 5 0.16184141598589719 1108 6 0.1389568778675141 1108 7 0.15651385825872666 1108 8 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-2.286880344559936 2316 86-87 -2.2310916603791853 2316 88-89 -2.367433442591526 2316 90-91 -2.5843334997250125 2316 92-93 -2.9209603687756704 2316 94-95 -2.96171711454555 2316 96-97 -2.9717453259588815 2316 98-99 -3.580718999688923 2316 100-101 -4.096547076900418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6188.0 3 1285.0 4 1181.0 5 1420.0 6 1646.0 7 2222.0 8 2849.0 9 3529.0 10 4631.0 11 6466.0 12 9840.0 13 14970.0 14 15051.0 15 15702.0 16 20940.0 17 29570.0 18 24448.0 19 14840.0 20 14200.0 21 14473.0 22 15738.0 23 17039.0 24 19089.0 25 22066.0 26 25109.0 27 29074.0 28 34300.0 29 40797.0 30 48370.0 31 59372.0 32 72786.0 33 92337.0 34 125809.0 35 184228.0 36 264339.0 37 384841.0 38 339033.0 39 55993.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.42380686822985 19.264011255904325 14.79130662683485 19.520875249030972 2 14.900925819857708 23.191612901005467 38.73374431717897 23.173716961957854 3 21.84954392948312 26.61296349469196 31.40499809233117 20.132494483493748 4 12.062838285386366 16.983469225280672 39.82905201167781 31.124640477655152 5 11.735793794884605 39.73832926491039 34.09794113539489 14.427935804810115 6 27.75894855246029 37.51115988027956 17.564176685977685 17.165714881282472 7 24.79565908304226 36.31471451272853 21.70129055351018 17.188335850719028 8 24.854062957090356 31.372641567412764 20.75843504554003 23.014860429956858 9 25.308543942036366 18.821069835339976 23.194598163953962 32.67578805866969 10-11 24.571928373748186 26.209262089916795 27.083203249642196 22.13560628669282 12-13 28.112338629562334 25.088298481440663 27.104407192500403 19.694955696496603 14-15 23.43321014993201 26.047327307008917 29.591380390851402 20.928082152207676 16-17 19.492768105670688 29.407925449183274 29.643428781014595 21.455877664131442 18-19 19.159850675960737 29.246820324354942 32.03462362651682 19.558705373167502 20-21 21.81023416780235 26.019344050852446 30.916281746821046 21.25414003452416 22-23 20.44090768370887 26.44656773583407 31.690955940952314 21.421568639504745 24-25 21.956018120023018 26.43017347807054 31.447634412304303 20.166173989602136 26-27 21.316054629926345 25.105733397637014 33.89899912029223 19.679212852144413 28-29 19.491168847967653 28.589014958230223 32.22183340436158 19.697982789440537 30-31 20.19538351188203 26.696806877003894 31.66072050774435 21.447089103369727 32-33 20.349980280169127 27.00446425497889 32.08967261146622 20.55588285338576 34-35 21.88768461926695 26.248450693529612 31.528728771080367 20.33513591612307 36-37 21.221778675687048 25.74470151036696 33.1059213668338 19.927598447112203 38-39 20.64678546443379 26.980157663621956 32.5168127759311 19.856244096013153 40-41 20.31622290677647 27.69283954885269 31.82378832869304 20.167149215677803 42-43 19.69442148030558 28.143352871856646 30.175273669824726 21.986951978013046 44-45 20.7224703778004 26.60251405757429 30.479662222924965 22.19535334170035 46-47 21.943536342495268 27.877294333229745 30.039924646614256 20.139244677660724 48-49 21.4482233611656 27.41387238305 30.169368067619732 20.96853618816467 50-51 19.559256214906387 28.354737549742847 31.58527085530641 20.500735380044357 52-53 20.839439079294277 29.41031279949657 28.807730955322864 20.942517165886294 54-55 19.456587448030756 27.66103887344988 30.088637681402886 22.793735997116478 56-57 21.293489922410167 27.643107505487798 29.064797489938215 21.99860508216382 58-59 22.643024759989274 27.224109291873887 28.997351077770045 21.135514870366794 60-61 20.86679233667979 27.69137998657732 31.149288225088373 20.292539451654523 62-63 20.354195211971973 27.71472800662189 30.710882773369853 21.220194008036284 64-65 19.880931318428786 29.385743387297165 29.861168081903518 20.87215721237053 66-67 20.599269725745565 27.661034122713257 28.89029204791884 22.849404103622334 68-69 19.747164603736504 28.096817179342953 29.75181571135256 22.404202505567984 70-71 21.55242077980361 27.949742320905983 28.785574517868007 21.71226238142239 72-73 20.830337131405386 26.693651017226 30.690448478917858 21.78556337245076 74-75 19.620891094466867 29.8224744749761 29.012128015664295 21.54450641489274 76-77 20.355110253316298 27.50064976969 28.97421871988948 23.17002125710423 78-79 20.328596669028865 27.69480169021084 29.857114592629497 22.119487048130797 80-81 21.227557304021285 27.73449424959173 29.086934357121052 21.95101408926594 82-83 20.377854311598266 27.166964661103993 30.41020469969917 22.04497632759857 84-85 19.972162374291553 27.689617884859253 29.855384176775345 22.48283556407385 86-87 20.063678454682147 28.006687497576056 29.404115607347652 22.525518440394148 88-89 19.9957209590981 27.694239304127148 28.62369541134628 23.686344325428472 90-91 20.247201183671734 26.400371824738304 29.410303953380573 23.942123038209388 92-93 21.048551625702046 27.658779502731086 29.305140132412628 21.987528739154243 94-95 20.394048669564953 27.007266802890907 29.508972488848485 23.089712038695655 96-97 18.805900812967952 26.845906743433247 30.854308235933676 23.49388420766513 98-99 20.096937742928166 27.613714906463777 28.596461184252632 23.692886166355425 100-101 19.808926167035192 25.996837671241334 29.92689585314936 24.267340308574113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7734.0 1 4877.0 2 2260.5 3 2481.5 4 2170.0 5 2144.0 6 3313.0 7 6085.0 8 9294.5 9 10259.0 10 8338.5 11 6644.5 12 6504.5 13 6601.0 14 6777.5 15 6999.0 16 7411.0 17 7635.5 18 7692.0 19 7885.0 20 8267.5 21 8955.5 22 9860.5 23 11034.0 24 12284.5 25 14098.0 26 16566.0 27 19205.5 28 21642.0 29 24093.0 30 26656.5 31 29630.0 32 32786.0 33 35701.0 34 38871.0 35 40778.0 36 43201.5 37 47293.5 38 48993.5 39 48081.0 40 47916.5 41 49310.5 42 50395.5 43 52191.5 44 54842.5 45 56717.0 46 64367.5 47 94362.5 48 158997.5 49 157742.5 50 91850.5 51 62331.0 52 53245.0 53 49859.0 54 47204.0 55 43933.0 56 40139.0 57 35578.5 58 32480.5 59 28986.5 60 24583.0 61 20396.5 62 16537.0 63 12612.0 64 8974.0 65 6422.5 66 4490.0 67 3534.5 68 2750.0 69 1762.5 70 1139.5 71 763.0 72 562.0 73 449.0 74 330.5 75 243.0 76 189.5 77 118.0 78 66.5 79 36.5 80 22.5 81 19.0 82 14.5 83 11.5 84 10.5 85 9.0 86 9.0 87 7.0 88 4.0 89 3.5 90 2.5 91 1.5 92 2.5 93 3.5 94 2.5 95 2.0 96 1.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29025772469184985 2 0.08822184355162672 3 0.09239715351927053 4 0.10688793517168137 5 0.10949136373974164 6 0.083751805821561 7 0.09436200526874997 8 0.09097263600089793 9 0.09578652278712256 10-11 0.08989196753868424 12-13 0.09374798909703765 14-15 0.10258982196969511 16-17 0.09016213465423767 18-19 0.10212316967919373 20-21 0.10455467371917454 22-23 0.11806302949684568 24-25 0.13100649039654147 26-27 0.13338887314278527 28-29 0.12518561708870862 30-31 0.11747357397200184 32-33 0.11253688395143477 34-35 0.385774080363419 36-37 0.5925747269961298 38-39 0.575750683891212 40-41 0.5373623928357576 42-43 0.5641826192161519 44-45 0.5873433092131407 46-47 0.5754068348350532 48-49 0.5976342202510393 50-51 0.5891362364345407 52-53 0.5928203334648147 54-55 0.5827504682487326 56-57 0.5543337998218861 58-59 0.5491760639795027 60-61 0.4961987486841633 62-63 0.5689473847086396 64-65 0.6053217027208776 66-67 0.6171844951583597 68-69 0.6190756649672337 70-71 0.6151950827620117 72-73 0.6082198590513598 74-75 0.6052480207802721 76-77 0.5886204628503023 78-79 0.5664913200217901 80-81 0.5599336273079025 82-83 0.5742279237853655 84-85 0.5844205922357901 86-87 0.5747928186633409 88-89 0.5874415518006146 90-91 0.6013919992219188 92-93 0.6093496488073104 94-95 0.608981239104283 96-97 0.6055181878958255 98-99 0.6099145436852857 100-101 0.5870977027444558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2035777.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.040995025617796 #Duplication Level Percentage of deduplicated Percentage of total 1 69.65735787422874 27.19492562265443 2 14.364351684392954 11.215971653132199 3 5.948161114699222 6.9666638547163675 4 3.0314184702338354 4.733983736678605 5 1.809789066038089 3.5327982962305255 6 1.1613660130775947 2.720453084369037 7 0.7822781381304531 2.13786418295804 8 0.5540747720267044 1.7305304334811908 9 0.424490474829913 1.4915277464631094 >10 2.0667096752876173 14.109688942075582 >50 0.1278844434372062 3.4689967808443107 >100 0.06188849952442465 4.372926075320654 >500 0.0034099246977442876 0.9692127972639366 >1k 0.0040413922343636 3.6211490185001796 >5k 0.0017681091025340753 4.749275833446452 >10k+ 0.0010103480585909 6.98403194186537 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 37460 1.8400836633874929 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20551 1.009491707588798 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19593 0.9624335081887653 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15720 0.7721867375454188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15157 0.7445314491714957 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12735 0.6255596757405159 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10348 0.5083071475903304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10082 0.49524088345629214 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9469 0.46512953039551974 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8826 0.4335445385226378 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8737 0.429172743380046 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8429 0.4140433849090544 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8366 0.4109487434036243 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7512 0.3689991585522383 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7121 0.3497927327010768 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6072 0.29826449557097856 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6052 0.2972820696962388 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5384 0.2644690454799322 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.2566587597757515 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5090 0.2500273851212584 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5027 0.24693274361582826 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5012 0.2461959242097735 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4808 0.23617518028742837 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4787 0.23514363311895164 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4102 0.20149554690911628 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3642 0.17889975179010276 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3485 0.17118770867339594 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3379 0.16598085153727546 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3196 0.15699165478340701 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3175 0.15596010761493032 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3081 0.15134270600365365 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2845 0.13975008068172495 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2599 0.1276662424224264 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2589 0.12717502948505657 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2353 0.11558240416312789 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 0.10664232870299645 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2042 0.10030568181092527 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.824258747397185E-5 0.0 10-11 0.0 0.0 0.0 2.456064686849296E-4 0.0 12-13 0.0 0.0 0.0 3.438490561589015E-4 0.0 14-15 0.0 0.0 0.0 4.175309967643804E-4 0.0 16-17 0.0 0.0 0.0 6.14016171712324E-4 0.0 18-19 0.0 0.0 0.0 9.087439341342396E-4 0.0 20-21 0.0 0.0 0.0 0.00132627493089862 0.0 22-23 0.0 0.0 0.0 0.0024069433931123104 0.0 24-25 0.0 0.0 0.0 0.004077067380169832 0.0 26-27 0.0 0.0 0.0 0.006975223710652002 0.0 28-29 0.0 0.0 0.0 0.016111784345731385 0.0 30-31 0.0 0.0 0.0 0.0401320969831175 0.0 32-33 0.0 0.0 0.0 0.0890077842514185 0.0 34-35 0.0 0.0 0.0 0.1605529485793385 0.0 36-37 0.0 0.0 0.0 0.2613498433276336 0.0 38-39 0.0 0.0 0.0 0.4108750614630188 0.0 40-41 0.0 0.0 0.0 0.5790418105715901 0.0 42-43 0.0 0.0 0.0 0.7492470933702464 0.0 44-45 0.0 0.0 0.0 0.9482128936519079 0.0 46-47 0.0 0.0 0.0 1.1884897019663745 0.0 48-49 0.0 0.0 0.0 1.4322541221361673 0.0 50-51 0.0 0.0 0.0 1.6957653023882282 0.0 52-53 0.0 0.0 0.0 1.953136320923166 0.0 54-55 0.0 0.0 0.0 2.23762229360092 0.0 56-57 0.0 0.0 0.0 2.596428783702734 0.0 58-59 0.0 0.0 0.0 2.9635367724460977 0.0 60-61 0.0 0.0 0.0 3.387772825805577 0.0 62-63 0.0 0.0 0.0 3.805082776748141 0.0 64-65 0.0 0.0 0.0 4.2142631535772335 0.0 66-67 0.0 0.0 0.0 4.689315185307624 0.0 68-69 0.0 0.0 0.0 5.240136812627316 0.0 70-71 0.0 0.0 0.0 5.830206353642859 0.0 72-73 0.0 0.0 0.0 6.412220002485537 0.0 74-75 0.0 0.0 0.0 7.037607753697974 0.0 76-77 0.0 0.0 0.0 7.641529499547348 0.0 78-79 0.0 0.0 0.0 8.425996560527013 0.0 80-81 0.0 0.0 0.0 9.25003082361182 0.0 82-83 0.0 0.0 0.0 10.015807232324562 0.0 84-85 0.0 0.0 0.0 10.838883630181499 0.0 86-87 0.0 0.0 0.0 11.679226162786986 0.0 88-89 0.0 0.0 0.0 12.568100533604614 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 215 0.0 27.658651 94-95 TTTGCGT 995 0.0 25.207312 3 GTACATG 13675 0.0 25.135412 1 TACATGG 13680 0.0 24.630184 2 GAGTACT 13060 0.0 24.566856 12-13 CATGGGG 3160 0.0 23.661583 4 ACATGGG 13930 0.0 23.304874 3 CTTTGCG 1090 0.0 23.010345 2 AGTACTT 13335 0.0 21.948748 12-13 GTACTTT 14640 0.0 21.738804 14-15 ATGGGAG 3440 0.0 21.461033 5 GTATAGG 345 1.8189894E-12 20.613298 1 CATGGGA 10390 0.0 20.58717 4 TTGCGTT 1250 0.0 20.443605 4 GATAGCG 70 4.810322E-4 20.38485 42-43 GTGTACG 215 1.0993135E-6 19.846292 1 GTACGTG 145 6.2267674E-4 19.618172 1 ATGGGAT 3525 0.0 19.063984 5 ACTTTTT 17055 0.0 18.96697 16-17 GTATACG 125 0.006098705 18.964233 1 >>END_MODULE