##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939032_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2035777 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09997656914289 33.0 31.0 34.0 30.0 34.0 2 32.303016489527096 34.0 31.0 34.0 30.0 34.0 3 32.38724919281434 34.0 31.0 34.0 30.0 34.0 4 35.969226491899654 37.0 35.0 37.0 35.0 37.0 5 35.9011984122033 37.0 35.0 37.0 35.0 37.0 6 35.97404381717644 37.0 35.0 37.0 35.0 37.0 7 35.920113057569665 37.0 35.0 37.0 35.0 37.0 8 35.91099418060033 37.0 35.0 37.0 35.0 37.0 9 37.53843765795566 39.0 37.0 39.0 35.0 39.0 10-11 37.46177233557506 39.0 37.0 39.0 34.0 39.0 12-13 37.40093463085593 39.0 37.0 39.0 34.0 39.0 14-15 38.52327563382433 40.0 38.0 41.0 34.0 41.0 16-17 38.55421173340695 40.0 38.0 41.0 34.0 41.0 18-19 38.73675456594705 40.0 38.0 41.0 34.0 41.0 20-21 38.745890880975665 40.0 38.0 41.0 34.0 41.0 22-23 38.676046050230454 40.0 38.0 41.0 34.0 41.0 24-25 38.581240725285724 40.0 38.0 41.0 34.0 41.0 26-27 38.37214930711959 40.0 38.0 41.0 34.0 41.0 28-29 38.1875271702156 40.0 38.0 41.0 33.5 41.0 30-31 37.992475354618904 40.0 37.0 41.0 33.0 41.0 32-33 37.72364654871335 40.0 36.5 41.0 33.0 41.0 34-35 37.589008521070824 40.0 36.0 41.0 33.0 41.0 36-37 37.42176034015513 40.0 36.0 41.0 32.5 41.0 38-39 37.23940416853123 40.0 36.0 41.0 32.0 41.0 40-41 37.0909397247341 40.0 36.0 41.0 31.5 41.0 42-43 36.84751424149108 40.0 35.0 41.0 31.0 41.0 44-45 36.48546697403498 39.5 35.0 41.0 30.5 41.0 46-47 36.013597510925806 39.0 35.0 41.0 29.5 41.0 48-49 35.56349074579387 39.0 35.0 41.0 27.0 41.0 50-51 35.230834467625876 39.0 35.0 40.5 26.0 41.0 52-53 35.01691049658189 39.0 35.0 40.0 26.0 41.0 54-55 34.78422538421448 38.0 34.5 40.0 25.0 41.0 56-57 34.47242674418662 38.0 34.0 40.0 23.5 41.0 58-59 34.17381397864304 38.0 34.0 40.0 23.0 41.0 60-61 33.67990477345996 37.5 33.0 40.0 22.0 41.0 62-63 33.40352184939706 37.0 33.0 40.0 22.0 41.0 64-65 33.16323865531441 36.5 33.0 39.5 20.5 41.0 66-67 32.66255218523443 36.0 32.5 39.0 18.5 40.0 68-69 32.21916251141457 35.5 32.0 38.5 17.0 40.0 70-71 31.708232041132206 35.0 31.5 37.5 12.5 40.0 72-73 31.17685163944774 35.0 31.0 37.0 8.0 39.0 74-75 30.743704246584965 35.0 31.0 36.5 7.0 39.0 76-77 28.803177116157613 32.5 28.0 35.0 2.0 36.5 78-79 29.746852921513508 34.0 29.5 35.0 2.0 37.0 80-81 29.72256735388994 34.0 30.0 35.0 2.0 37.0 82-83 29.400854071934205 34.0 29.5 35.0 2.0 36.5 84-85 29.00535815072083 34.0 29.0 35.0 2.0 36.0 86-87 28.508282586943462 34.0 29.0 35.0 2.0 36.0 88-89 27.921554521934375 34.0 27.5 35.0 2.0 35.0 90-91 27.434725660030544 33.0 26.5 35.0 2.0 35.0 92-93 27.11823765569608 33.0 26.0 35.0 2.0 35.0 94-95 26.73176850902628 33.0 25.0 35.0 2.0 35.0 96-97 26.10112084968049 33.0 24.5 34.5 2.0 35.0 98-99 25.284727158230005 32.0 20.5 34.0 2.0 35.0 100-101 21.190329540023292 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1789391503638953 1101 2 0.13853381470156734 1101 3 0.15138064539836193 1101 4 0.019464739738872083 1101 5 0.07127240593049322 1101 6 0.07994108510609266 1101 7 0.07167273681805852 1101 8 0.11623980193902383 1101 9 0.16895224314514223 1101 10-11 0.2423426744067072 1101 12-13 0.12172671397553358 1101 14-15 0.30417146644128934 1101 16-17 0.31940493080550425 1101 18-19 0.30437792807044417 1101 20-21 0.2652407447869578 1101 22-23 0.27931113146672004 1101 24-25 0.32689107916468885 1101 26-27 0.19702181042917033 1101 28-29 0.3356082488145873 1101 30-31 0.2841209196099115 1101 32-33 0.4254970368008557 1101 34-35 0.4226597576411848 1101 36-37 0.37633875920146664 1101 38-39 0.4088740994032065 1101 40-41 0.41400760735152886 1101 42-43 0.44144237182258905 1101 44-45 0.48350201256048564 1101 46-47 0.4435211383622075 1101 48-49 0.2947562414446807 1101 50-51 0.18632047261003493 1101 52-53 0.4122679000152303 1101 54-55 0.4266137194254185 1101 56-57 0.2760477526086831 1101 58-59 0.4412856311783955 1101 60-61 0.4596575905808109 1101 62-63 0.36358920120145655 1101 64-65 0.32967749330185114 1101 66-67 0.41584277013363646 1101 68-69 0.37801151845265935 1101 70-71 0.18617210902231562 1101 72-73 0.003007022033532536 1101 74-75 0.003128507507124567 1101 76-77 -0.1015579801142188 1101 78-79 0.34111198388701425 1101 80-81 0.30651174383011437 1101 82-83 0.17173313254680878 1101 84-85 0.18627862326937006 1101 86-87 0.4244707594007089 1101 88-89 0.4898005888114447 1101 90-91 0.5297326545330421 1101 92-93 0.4910238018077884 1101 94-95 0.5212933530298827 1101 96-97 0.3862900516547363 1101 98-99 0.30935189275391295 1101 100-101 0.7884758115226269 1102 1 0.2385721039128228 1102 2 0.1994329633980314 1102 3 0.22380817560604527 1102 4 0.18406287987225056 1102 5 0.1442435047621089 1102 6 0.23907823734033684 1102 7 0.23192399620122472 1102 8 0.18278872784186007 1102 9 0.2666800428850067 1102 10-11 0.2981810740961848 1102 12-13 0.27839375692329327 1102 14-15 0.32708712405366924 1102 16-17 0.2612642191003971 1102 18-19 0.24185780490974906 1102 20-21 0.3524940744575744 1102 22-23 0.3547149431756651 1102 24-25 0.3067909958082353 1102 26-27 0.2893251845584217 1102 28-29 0.28738370242329125 1102 30-31 0.2739314407629365 1102 32-33 0.26824110467381246 1102 34-35 0.3796994856956104 1102 36-37 0.5181820879879169 1102 38-39 0.422319707229704 1102 40-41 0.38730031530197806 1102 42-43 0.44837363638842476 1102 44-45 0.4375010928034513 1102 46-47 0.5096688522321386 1102 48-49 0.3420925883632506 1102 50-51 0.521661580689269 1102 52-53 0.46959450996539687 1102 54-55 0.6230209115473144 1102 56-57 0.5176355068278085 1102 58-59 0.5939265127660676 1102 60-61 0.5892950928676939 1102 62-63 0.48571614213069836 1102 64-65 0.4646263978511982 1102 66-67 0.3878082531089504 1102 68-69 0.4340676236319396 1102 70-71 0.3746226242586417 1102 72-73 0.5845115921805792 1102 74-75 0.5831458405342396 1102 76-77 0.40510455858203187 1102 78-79 0.5986686999206512 1102 80-81 0.7678370447669458 1102 82-83 0.7399995128691224 1102 84-85 0.6648287936802184 1102 86-87 0.627771680659162 1102 88-89 0.7488613633981238 1102 90-91 1.101100851514527 1102 92-93 1.0010468735849791 1102 94-95 0.8256699562792846 1102 96-97 0.7451245849169617 1102 98-99 0.8281119612852699 1102 100-101 1.2053330284179076 1103 1 0.0012477178789183085 1103 2 0.12552734156618328 1103 3 0.1210496980671536 1103 4 0.09176667947022565 1103 5 0.09484135697326224 1103 6 0.05105057609230812 1103 7 0.13539721985093678 1103 8 0.1507363113062894 1103 9 0.09014458142721793 1103 10-11 0.07118568430419714 1103 12-13 0.09516366166022294 1103 14-15 0.10722963628928994 1103 16-17 0.103848694061071 1103 18-19 0.1393809105730881 1103 20-21 0.10310858678059986 1103 22-23 0.13971619951197312 1103 24-25 0.1054811286521371 1103 26-27 -0.022775655548201712 1103 28-29 0.21354021461522166 1103 30-31 0.08973437622002223 1103 32-33 0.08091221221619804 1103 34-35 0.06572875286917679 1103 36-37 0.027822830428043233 1103 38-39 0.13722941488570228 1103 40-41 0.136238574566093 1103 42-43 0.05222881358645992 1103 44-45 0.01648545414762026 1103 46-47 0.10780401216921831 1103 48-49 0.1202074830494908 1103 50-51 0.09559246580550962 1103 52-53 0.2608155869187243 1103 54-55 0.31560552306628153 1103 56-57 0.22188274288793508 1103 58-59 0.16402693773547838 1103 60-61 0.015422381584706102 1103 62-63 0.16176545606183623 1103 64-65 0.023757777854697792 1103 66-67 0.18808478365431114 1103 68-69 0.2972715816375313 1103 70-71 0.2073837783400947 1103 72-73 0.36087143967227675 1103 74-75 0.3833037019221095 1103 76-77 0.23746696244499432 1103 78-79 0.2678843637032067 1103 80-81 0.2197783574745138 1103 82-83 0.3705613956256322 1103 84-85 0.30896552676883715 1103 86-87 0.15041632320417264 1103 88-89 0.2941528334208492 1103 90-91 0.4676758875659175 1103 92-93 0.622382331765369 1103 94-95 0.4507618600111485 1103 96-97 0.3490780840866883 1103 98-99 0.4929299017676989 1103 100-101 0.8629472457750147 1104 1 0.030827161170755346 1104 2 0.08058223437393508 1104 3 0.12505211853741116 1104 4 0.06706707711891369 1104 5 0.03460838671046673 1104 6 0.04308895783504596 1104 7 -0.08953146272168055 1104 8 0.08398752154142386 1104 9 0.05626232001642961 1104 10-11 -0.005340076415031092 1104 12-13 0.10540311532550106 1104 14-15 0.06241851941515364 1104 16-17 0.07627802497393077 1104 18-19 0.13609382551084792 1104 20-21 0.0737387735025905 1104 22-23 0.022355808778911523 1104 24-25 0.09081134995228268 1104 26-27 0.02121531091098916 1104 28-29 0.08720139200111277 1104 30-31 -0.02234312205106903 1104 32-33 0.04672415032131738 1104 34-35 0.182332746645109 1104 36-37 0.13152053098129812 1104 38-39 0.19247448749981544 1104 40-41 0.3702515845937526 1104 42-43 0.27361863896682337 1104 44-45 0.2994006080203704 1104 46-47 0.4530062943268902 1104 48-49 0.3764682887203108 1104 50-51 0.41012285135184356 1104 52-53 0.26270549874444526 1104 54-55 0.43833417106075245 1104 56-57 0.3242405402599928 1104 58-59 0.3609808191324362 1104 60-61 0.5356106163703132 1104 62-63 0.5543527658820224 1104 64-65 0.4350691540649336 1104 66-67 0.6596583054385476 1104 68-69 0.5762988551506751 1104 70-71 0.3649844871921033 1104 72-73 0.39167840716881486 1104 74-75 0.3056109287547528 1104 76-77 0.27469249840626375 1104 78-79 0.5503035372855045 1104 80-81 0.5251293256626184 1104 82-83 0.5868617500516358 1104 84-85 0.4978400080966381 1104 86-87 0.36632227064538725 1104 88-89 0.548699859700239 1104 90-91 0.6535388284372878 1104 92-93 0.5349698576989788 1104 94-95 0.7408952019890158 1104 96-97 0.7556338845707806 1104 98-99 0.7800733146307692 1104 100-101 1.572134227102815 1105 1 -0.04026814661493461 1105 2 -0.03614495715370225 1105 3 -0.004564037826398248 1105 4 0.03488004691169522 1105 5 -0.0036693070826601115 1105 6 0.028523415910392202 1105 7 -0.0494870335598776 1105 8 0.05568778090373172 1105 9 -0.07068001432821092 1105 10-11 -0.025830380206507186 1105 12-13 -0.05941198681543369 1105 14-15 0.06640382990559601 1105 16-17 -0.023091600911136823 1105 18-19 -0.05724064703176168 1105 20-21 0.06291309663405542 1105 22-23 -0.039634430014722 1105 24-25 -0.0848433197224594 1105 26-27 -0.01443429522312556 1105 28-29 -0.03199375140297178 1105 30-31 0.1329355142105868 1105 32-33 0.006382120208449749 1105 34-35 0.11350777636589271 1105 36-37 0.20315259503980343 1105 38-39 0.08254507304639702 1105 40-41 0.104085209019523 1105 42-43 0.05955798341128826 1105 44-45 0.08193293919899958 1105 46-47 0.27799413344258284 1105 48-49 -0.13281303446777315 1105 50-51 -0.06547622945303999 1105 52-53 0.03642708578178855 1105 54-55 0.010314288014910744 1105 56-57 0.052409787270612185 1105 58-59 -0.03260659456197601 1105 60-61 0.0011128871009589147 1105 62-63 -0.043919759535263836 1105 64-65 -0.18907119721689014 1105 66-67 -0.05061334355639957 1105 68-69 -0.04732098931581419 1105 70-71 -0.010599051792965497 1105 72-73 -0.17473024257465042 1105 74-75 -0.05935725900091526 1105 76-77 -0.17101085673978034 1105 78-79 -0.06215948209433364 1105 80-81 -0.2528856563371775 1105 82-83 -0.1133889633519658 1105 84-85 -0.2701242913211068 1105 86-87 -0.10490623027401469 1105 88-89 -0.08986476806138555 1105 90-91 0.06395438996612057 1105 92-93 0.10385379778356096 1105 94-95 0.050733304974937 1105 96-97 -0.21165110889798555 1105 98-99 -0.04334867958651856 1105 100-101 0.5406845815506607 1106 1 -0.006560632054686266 1106 2 -0.12407217702610751 1106 3 -0.17233509608955444 1106 4 -0.057052626638672166 1106 5 -0.07070686679831795 1106 6 0.027220894817403973 1106 7 -0.062455120786516716 1106 8 -0.002455089756125517 1106 9 -0.12677523928991263 1106 10-11 -0.03910233030403987 1106 12-13 0.03597956471467256 1106 14-15 -0.020187777389324424 1106 16-17 -0.05163873123773044 1106 18-19 -0.021332485841249138 1106 20-21 0.04714494136226932 1106 22-23 0.13773454280546105 1106 24-25 0.07205649385797841 1106 26-27 -0.04894078669030222 1106 28-29 0.012895916806456853 1106 30-31 -0.031998484005740124 1106 32-33 0.12171877762124694 1106 34-35 0.09508009749069402 1106 36-37 -0.08162894580037516 1106 38-39 0.06931980333192911 1106 40-41 0.19945964860327337 1106 42-43 0.23984605296229233 1106 44-45 0.3008801669630259 1106 46-47 0.21041855400188325 1106 48-49 0.26999790469623264 1106 50-51 0.27789347012157606 1106 52-53 0.22848553213784584 1106 54-55 0.18230331523095344 1106 56-57 0.1264166904310926 1106 58-59 0.13489243408341878 1106 60-61 0.31529891710927416 1106 62-63 0.2573788358759046 1106 64-65 0.07758828914288074 1106 66-67 0.21603859341526999 1106 68-69 0.11879071344363723 1106 70-71 0.19413469135470507 1106 72-73 0.214882478848903 1106 74-75 0.37622783870829934 1106 76-77 0.20981714504791427 1106 78-79 0.4611342807018808 1106 80-81 0.41942145176402335 1106 82-83 0.4643352585074396 1106 84-85 0.3541099490795965 1106 86-87 0.39440702705543274 1106 88-89 0.29113158508087267 1106 90-91 0.7180649562910162 1106 92-93 0.4832669184187566 1106 94-95 0.594012814526419 1106 96-97 0.3235479131039547 1106 98-99 0.32696252128961234 1106 100-101 0.5815416182849766 1107 1 -0.15127913557646977 1107 2 -0.0907684793970347 1107 3 -0.12094786914379796 1107 4 -0.07094387017516368 1107 5 -0.1439665248509172 1107 6 -0.1441096168814724 1107 7 -0.12801894170275574 1107 8 -0.07971014671779386 1107 9 -0.09453039383255657 1107 10-11 -0.09622576629781321 1107 12-13 -0.19615818808412655 1107 14-15 -0.049683968774210996 1107 16-17 -0.13633446093645318 1107 18-19 -0.13010743059464858 1107 20-21 -0.08736797185374456 1107 22-23 -0.05819660149413863 1107 24-25 -0.05954340540690595 1107 26-27 0.01843370347373252 1107 28-29 -0.05672860342040309 1107 30-31 -0.07018813017120351 1107 32-33 -0.013494821224341536 1107 34-35 -0.1098302216483873 1107 36-37 0.031091375290323242 1107 38-39 -0.08519992083918027 1107 40-41 -0.06036054612498987 1107 42-43 -0.07405162204771898 1107 44-45 -0.08683579255367846 1107 46-47 -0.24835939703415022 1107 48-49 -0.1461733824461433 1107 50-51 -0.13149975854712181 1107 52-53 -0.09992669722279146 1107 54-55 -0.15639446662529366 1107 56-57 -0.1174029345819676 1107 58-59 0.0327451104635017 1107 60-61 -0.025756825284830143 1107 62-63 -0.11447516326852991 1107 64-65 -0.27492841297492987 1107 66-67 -0.14652254254558983 1107 68-69 -0.01037791634201568 1107 70-71 0.01266401295992381 1107 72-73 0.03176327333547846 1107 74-75 -0.16311257302428217 1107 76-77 -0.29553933600461946 1107 78-79 0.06340148600239814 1107 80-81 0.09149306141645752 1107 82-83 0.14284216112951142 1107 84-85 -0.029966313904093766 1107 86-87 -0.16793267893998376 1107 88-89 -0.002084590296785649 1107 90-91 0.19174310367180425 1107 92-93 0.28024624505087203 1107 94-95 0.07109742511918071 1107 96-97 -0.04430403388296256 1107 98-99 -0.1777552479106781 1107 100-101 0.21154091846180378 1108 1 -0.035021363702668395 1108 2 -0.017930178453831047 1108 3 0.013060458827908406 1108 4 -0.03788953924849636 1108 5 0.035652480596887415 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-1.2212568939117148 2316 56-57 -1.2910576628820962 2316 58-59 -1.1925762300727385 2316 60-61 -1.0723574933634055 2316 62-63 -1.202173159546298 2316 64-65 -1.1643869398704965 2316 66-67 -1.2268569063622898 2316 68-69 -1.3472810724720574 2316 70-71 -1.0974529018176469 2316 72-73 -1.1417975879325404 2316 74-75 -1.3159296417889728 2316 76-77 -1.1874273142258254 2316 78-79 -1.1656182566786946 2316 80-81 -1.270572929366498 2316 82-83 -1.53319826865415 2316 84-85 -1.5593226633515442 2316 86-87 -1.6885002396849949 2316 88-89 -2.075300259148687 2316 90-91 -2.3081598456221286 2316 92-93 -2.552678961821183 2316 94-95 -2.460928999785448 2316 96-97 -2.6338216538751205 2316 98-99 -3.031145829863682 2316 100-101 -3.7659800840298843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 28.0 5 88.0 6 238.0 7 470.0 8 920.0 9 1756.0 10 3025.0 11 4527.0 12 6622.0 13 9338.0 14 10931.0 15 14790.0 16 19785.0 17 28468.0 18 27049.0 19 16347.0 20 13557.0 21 13992.0 22 14645.0 23 15596.0 24 17994.0 25 20777.0 26 24281.0 27 28885.0 28 34796.0 29 42226.0 30 50732.0 31 61520.0 32 75672.0 33 95774.0 34 130602.0 35 189596.0 36 283926.0 37 431939.0 38 320528.0 39 24350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.253397898794724 19.96796058994734 15.271000832057954 19.50764067919998 2 15.103815398248432 23.658878158069378 38.11370302346475 23.12360342021744 3 21.770802990700847 26.701942305075654 31.610191096569025 19.917063607654473 4 12.245545558280696 17.491994457153215 39.20291859078868 31.059541393777412 5 11.575232749884687 40.11648669372605 34.06378621543014 14.244494340959116 6 27.88439008791238 37.86397036610592 17.157380204216864 17.09425934176484 7 24.699218038125 36.38438787745416 21.531778775376676 17.384615309044165 8 25.04144609159058 31.855306352316585 20.404936297050217 22.698311259042615 9 25.058736786986003 18.83678811579068 23.0616614688151 33.04281362840822 10-11 24.614582049016175 26.5151340249939 26.873326498924 21.99695742706593 12-13 28.042020319514364 25.169800032125327 27.1055474150656 19.682632233294708 14-15 23.22615791233031 26.239651508159056 29.486696138290508 21.047494441220124 16-17 19.464216241069536 29.5803270377968 29.557853504986912 21.397603216146756 18-19 19.147283813502167 29.292894064526713 31.99181442761167 19.56800769435945 20-21 21.754936812823804 26.022668494633745 30.950000908743935 21.27239378379852 22-23 20.395529569299583 26.473601971139278 31.75271653034689 21.37815192921425 24-25 21.835372931318116 26.608882014090934 31.524204271882432 20.031540782708518 26-27 21.26780094283411 25.206935730190487 33.97540595065177 19.549857376323633 28-29 19.463453020640276 28.673474550503318 32.24388034642301 19.61919208243339 30-31 20.253569030399696 26.702237032838077 31.711798492663977 21.33239544409825 32-33 20.35714127824413 27.069418703522047 32.12262443283326 20.450815585400562 34-35 21.8439661615493 26.25945513934426 31.647149931118122 20.249428767988313 36-37 21.146001279213372 25.799017108250734 33.232578086544386 19.822403525991515 38-39 20.588330659959077 27.034298613857494 32.61546888855591 19.76190183762752 40-41 20.280263719248985 27.645500338822693 31.895901485735674 20.178334456192644 42-43 19.714165997793458 28.149533196906123 30.296567083971503 21.83973372132892 44-45 20.732687352082703 26.541655573381224 30.570900584841283 22.154756489694787 46-47 21.899614445829123 28.00042342679997 30.041492879110663 20.05846924826024 48-49 21.436967677634 27.448148688483442 30.1851679926986 20.929715641183964 50-51 19.492653137613488 28.424863945628353 31.551547893251048 20.53093502350711 52-53 20.779019509504234 29.518901137010584 28.722006388715464 20.980072964769718 54-55 19.46947037912306 27.72675985631039 29.99044099623878 22.813328768327768 56-57 21.266376425315737 27.702911468201087 28.938704976036178 22.092007130447 58-59 22.56159397063963 27.337645703361108 28.845305084583412 21.255455241415845 60-61 20.84509616003372 27.933174536196265 30.870610461846198 20.35111884192382 62-63 20.262067014216193 28.010312524407144 30.39286719517904 21.334753266197623 64-65 19.802129021387966 29.591172091556466 29.570025312277266 21.036673574778302 66-67 20.525884421551453 27.903528500408818 28.625227587944796 22.945359490094937 68-69 19.73980041704634 28.323439330564486 29.412254481774486 22.524505770614688 70-71 21.464924694600636 28.309313839384174 28.404167057590296 21.82159440842489 72-73 20.830523185987463 27.140669140087542 30.146351982559978 21.882455691365017 74-75 19.634493365432462 30.25842221422091 28.363813914785364 21.74327050556127 76-77 20.328253610231485 27.882178687983977 28.55050463195103 23.239063069833506 78-79 20.30075538836826 28.181983854789383 29.261180268536684 22.256080488305678 80-81 21.120387940329415 28.21794823303338 28.514174194914276 22.147489631722923 82-83 20.287782011487508 27.840008999021016 29.64936729317602 22.22284169631546 84-85 19.83645556463208 28.35647028137168 29.053820727908803 22.753253426087436 86-87 19.938345486646604 28.642415137110326 28.522259429086976 22.896979947156083 88-89 19.821481035443544 28.422943515453035 27.6872632313541 24.068312217749327 90-91 20.051489648717578 27.239832987629324 28.40388249793058 24.304794865722517 92-93 20.790538452885556 28.622363844369985 28.271490443206698 22.31560725953776 94-95 20.130397386354204 27.982902842501904 28.445772793385522 23.44092697775837 96-97 18.393400418663152 28.079216393047236 29.649289174023234 23.878094014266377 98-99 19.752433591694967 28.75678426468125 27.326863403997585 24.163918739626197 100-101 19.330071997292425 27.533622396625162 28.553383460123953 24.58292214595846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5558.0 1 3740.0 2 2100.5 3 2272.5 4 2016.5 5 2049.0 6 3234.0 7 5798.0 8 8588.0 9 9400.5 10 7843.0 11 6296.0 12 5958.0 13 6037.5 14 6312.0 15 6533.5 16 6686.0 17 6906.0 18 7137.0 19 7384.5 20 7884.5 21 8693.0 22 9590.0 23 10951.5 24 12391.5 25 14156.5 26 16686.5 27 19212.0 28 21757.0 29 24363.5 30 27133.0 31 30079.5 32 33000.0 33 35992.5 34 38922.5 35 40637.0 36 43125.5 37 47315.5 38 49332.0 39 48658.5 40 48381.5 41 49704.0 42 50690.0 43 52387.5 44 55056.0 45 56945.5 46 63096.5 47 94679.5 48 159766.5 49 158248.0 50 96328.5 51 66057.0 52 53769.0 53 49426.0 54 47388.0 55 44605.5 56 40576.0 57 36056.5 58 32846.0 59 28953.5 60 24276.0 61 20055.0 62 16169.5 63 12135.5 64 8542.0 65 6048.5 66 4181.0 67 3306.0 68 2511.5 69 1560.5 70 964.5 71 635.5 72 444.5 73 339.5 74 251.0 75 185.5 76 139.0 77 60.0 78 22.5 79 13.0 80 5.5 81 5.0 82 3.5 83 2.0 84 2.5 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4064295843798215 2 0.0 3 0.0 4 0.0 5 3.929703498958874E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0016455633401890285 16-17 0.0024069433931123104 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022350188650328597 36-37 0.007417315354284875 38-39 0.01336099189646017 40-41 0.002529746627454775 42-43 7.368194060547889E-4 44-45 5.403342311068452E-4 46-47 2.947277624219156E-4 48-49 1.964851749479437E-4 50-51 4.912129373698592E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.701671155534226E-4 60-61 0.002750792449271212 62-63 0.0 64-65 2.947277624219156E-4 66-67 0.0012525929902931412 68-69 0.0015718813995835496 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12587331520102643 78-79 1.473638812109578E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1472656386234838 88-89 0.4360742851500926 90-91 0.33527739040179744 92-93 0.0 94-95 0.0 96-97 5.648948779753382E-4 98-99 0.0 100-101 2.947277624219156E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2035777.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.6984286995185 #Duplication Level Percentage of deduplicated Percentage of total 1 68.13652329274865 25.686398651847668 2 15.096581998420477 11.38234840147778 3 6.180957894589393 6.990372014517127 4 3.227826218550605 4.867359062178657 5 1.8947371353122175 3.5714306399948783 6 1.2146636883230344 2.7474547468884047 7 0.8037150589473034 2.1209156391117965 8 0.600583547830879 1.8112844844805 9 0.4459765289927111 1.513135294190142 >10 2.183237218422654 14.45133620382192 >50 0.13571821717912658 3.5334359495310803 >100 0.06762318727514233 4.514230793477484 >500 0.004429719295231371 1.1371763265165478 >1k 0.004560005156855823 3.8449261162136623 >5k 0.0016937162011178772 4.395808061456593 >10k+ 0.0011725727546200689 7.432387614295775 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38403 1.8864050433814707 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20062 0.9854713949514116 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18920 0.9293748775037738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15464 0.7596116863487503 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15153 0.7443349639965477 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12485 0.6132793523062693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10403 0.5110088187458647 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10309 0.5063914171345879 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10125 0.4973530990869825 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9600 0.4715644198750649 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9066 0.44533364901951444 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8965 0.4403723983520788 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8627 0.42376940106897754 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7630 0.3747954712132026 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7109 0.34920327717623295 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6537 0.321105897158677 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6047 0.2970364632275539 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5243 0.2575429430630172 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5231 0.2569534875381734 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5226 0.25670788106948844 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5170 0.25395708862021726 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5048 0.24796429078430496 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4936 0.24246270588576252 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4696 0.23067359538888593 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3828 0.18803631242518212 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3615 0.17757347685920413 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3561 0.1749209269974069 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3531 0.1734472881852973 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3369 0.1654896385999056 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3352 0.16465457660637683 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3327 0.16342654426295217 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2904 0.14264823701220714 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2679 0.1315959459213853 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2550 0.12525929902931413 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2425 0.11911913731219087 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2142 0.10521781118462387 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2081 0.10222141226666771 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.824258747397185E-5 0.0 10-11 0.0 0.0 0.0 2.2104582181643666E-4 0.0 12-13 0.0 0.0 0.0 2.947277624219156E-4 0.0 14-15 0.0 0.0 0.0 3.684097030273944E-4 0.0 16-17 0.0 0.0 0.0 5.648948779753381E-4 0.0 18-19 0.0 0.0 0.0 8.105013466602678E-4 0.0 20-21 0.0 0.0 0.0 0.001228032343424648 0.0 22-23 0.0 0.0 0.0 0.0023332614525068316 0.0 24-25 0.0 0.0 0.0 0.004052506733301339 0.0 26-27 0.0 0.0 0.0 0.006901541770046523 0.0 28-29 0.0 0.0 0.0 0.01625914822694234 0.0 30-31 0.0 0.0 0.0 0.04052506733301339 0.0 32-33 0.0 0.0 0.0 0.0900393314198952 0.0 34-35 0.0 0.0 0.0 0.1626897248568974 0.0 36-37 0.0 0.0 0.0 0.2650830616516445 0.0 38-39 0.0 0.0 0.0 0.41613103989287625 0.0 40-41 0.0 0.0 0.0 0.5865328078664804 0.0 42-43 0.0 0.0 0.0 0.7595625650550134 0.0 44-45 0.0 0.0 0.0 0.9610089906703927 0.0 46-47 0.0 2.4560646868492963E-5 0.0 1.2051663811900812 0.0 48-49 0.0 4.9121293736985926E-5 0.0 1.4527868229182275 0.0 50-51 0.0 4.9121293736985926E-5 0.0 1.7181154910385568 0.0 52-53 0.0 4.9121293736985926E-5 0.0 1.9781144987884234 0.0 54-55 0.0 4.9121293736985926E-5 0.0 2.2645407625687883 0.0 56-57 0.0 4.9121293736985926E-5 0.0 2.6260734844730047 0.0 58-59 0.0 4.9121293736985926E-5 0.0 2.9942621416785826 0.0 60-61 0.0 4.9121293736985926E-5 0.0 3.4206104106687523 0.0 62-63 0.0 4.9121293736985926E-5 0.0 3.839664167538979 0.0 64-65 0.0 4.9121293736985926E-5 0.0 4.250293622533313 0.0 66-67 0.0 4.9121293736985926E-5 0.0 4.728317492534792 0.0 68-69 0.0 4.9121293736985926E-5 0.0 5.28080924384154 0.0 70-71 0.0 4.9121293736985926E-5 0.0 5.875078655471596 0.0 72-73 0.0 4.9121293736985926E-5 0.0 6.461734266572419 0.0 74-75 0.0 4.9121293736985926E-5 0.0 7.092451678155318 0.0 76-77 0.0 4.9121293736985926E-5 0.0 7.700917143675364 0.0 78-79 0.0 4.9121293736985926E-5 0.0 8.489092862332171 0.0 80-81 0.0 4.9121293736985926E-5 0.0 9.316442812744224 0.0 82-83 0.0 4.9121293736985926E-5 0.0 10.08546122684361 0.0 84-85 0.0 4.9121293736985926E-5 0.0 10.90861130664115 0.0 86-87 0.0 4.9121293736985926E-5 0.0 11.749273127655927 0.0 88-89 0.0 4.9121293736985926E-5 0.0 12.640701805747879 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 990 0.0 32.592606 3 CTTTGCG 1005 0.0 32.10615 2 GTAAGCG 265 0.0 27.754358 94-95 TTGCGTT 1330 0.0 24.260662 4 GAGTACT 13880 0.0 23.45316 12-13 GTACATG 12825 0.0 22.587938 1 TACATGG 12995 0.0 21.945402 2 AGTACTT 14210 0.0 21.33897 12-13 GTACTTT 15235 0.0 21.289368 14-15 CATGGGG 3120 0.0 20.987942 4 ACATGGG 13120 0.0 20.976814 3 GCTTTGC 1640 0.0 20.627419 1 ACTTTTT 17320 0.0 18.876757 16-17 CATGGGA 9665 0.0 18.803656 4 ATGGGAT 3130 0.0 18.79848 5 CCTATAC 390 1.6370905E-11 18.250385 3 TACTTTT 16535 0.0 18.166288 14-15 GTCGGGA 425 3.6379788E-12 17.863907 2 GTGTACG 215 2.3333354E-5 17.728905 1 ATGGGAG 3405 0.0 16.862177 5 >>END_MODULE