FastQCFastQC Report
Fri 27 May 2016
SRR939031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334710
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT211550.6343879977569263No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT109220.32752473228556606No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT90190.27045830072180194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88910.2666198859870873No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC79800.239301168617361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70000.20991330580470263No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63250.18967166560210633No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51760.15521589583502013No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC49470.14834873197369486No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47360.14202134518443882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44510.13347487487667592No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG43420.13020622482914557No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA41540.12456855318753356No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC40140.1203702870714395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG106150.024.1469081
TACATGG105300.023.8390542
ACATGGG111200.021.8927273
GAGTACT86500.020.71567212-13
AGAGTAC137400.019.96699510-11
TTTGCGT9600.019.734693
AGTACTT90100.019.15234612-13
CATGGGG78300.019.1146724
GTACTTT92300.019.05574414-15
GTATACG1801.240892E-418.4592171
GTATAGG9200.018.057931
GTATAGA8800.017.2605671
CCACGCG2203.0046325E-517.215259
AGTACAT79850.016.2523882
GAGTACA86100.016.0978341
ACTTTTT111550.016.0025616-17
TACTTTT107650.015.85477414-15
CTTTGCG12650.015.7253482
ACCGTCC6050.014.8677168
CATATAG6100.014.7524883
Produced by FastQC (version 0.11.5)