##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939031_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3334710 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81886760767803 33.0 31.0 34.0 30.0 34.0 2 31.89391431338857 33.0 31.0 34.0 30.0 34.0 3 31.95619948961079 34.0 31.0 34.0 30.0 34.0 4 35.48158640481481 37.0 35.0 37.0 33.0 37.0 5 35.518000365848906 37.0 35.0 37.0 33.0 37.0 6 35.601083752410254 37.0 35.0 37.0 33.0 37.0 7 35.52483784197127 37.0 35.0 37.0 33.0 37.0 8 35.512882679453384 37.0 35.0 37.0 33.0 37.0 9 37.123400235702654 39.0 37.0 39.0 33.0 39.0 10-11 37.0072123213113 39.0 37.0 39.0 33.0 39.0 12-13 36.905854781975044 39.0 37.0 39.0 33.0 39.0 14-15 38.12256283155057 40.0 38.0 41.0 33.0 41.0 16-17 38.04073382692948 40.0 38.0 41.0 33.0 41.0 18-19 37.94443189962546 40.0 38.0 41.0 33.0 41.0 20-21 38.04789022133859 40.0 38.0 41.0 33.0 41.0 22-23 37.97711825016268 40.0 38.0 41.0 33.0 41.0 24-25 37.89493119341712 40.0 38.0 41.0 32.5 41.0 26-27 37.77395215775884 40.0 38.0 41.0 32.0 41.0 28-29 37.65361590663056 40.0 37.5 41.0 32.0 41.0 30-31 37.4819333615217 40.0 37.0 41.0 32.0 41.0 32-33 37.28506226928279 40.0 37.0 41.0 31.0 41.0 34-35 36.960074189359794 40.0 36.0 41.0 30.5 41.0 36-37 36.703532391122465 39.5 36.0 41.0 30.0 41.0 38-39 36.472497758425774 39.0 35.5 41.0 30.0 41.0 40-41 36.34310599722315 39.0 35.0 41.0 30.0 41.0 42-43 36.1339768675537 39.0 35.0 41.0 29.5 41.0 44-45 36.09722554584957 39.0 35.0 41.0 29.0 41.0 46-47 35.91665272242564 39.0 35.0 41.0 28.0 41.0 48-49 35.68364295545939 39.0 35.0 41.0 27.0 41.0 50-51 34.5603221869368 38.0 34.0 40.0 25.5 40.5 52-53 34.669788977152436 38.0 34.0 39.5 26.0 40.5 54-55 35.16370973787826 39.0 35.0 40.0 26.0 41.0 56-57 35.030247607738005 38.5 34.5 40.0 26.0 41.0 58-59 34.8020024229993 38.0 34.0 40.0 26.0 41.0 60-61 34.45651331000297 38.0 34.0 40.0 25.5 41.0 62-63 34.06869248000575 37.0 34.0 40.0 24.5 41.0 64-65 33.67124817450393 36.5 33.0 39.5 23.0 41.0 66-67 33.22338509195703 36.0 33.0 39.0 22.0 41.0 68-69 32.758890128376976 35.5 32.0 39.0 21.5 40.0 70-71 32.29897487337729 35.0 32.0 38.0 20.0 40.0 72-73 31.85170329653853 35.0 31.5 37.0 19.0 39.5 74-75 31.398422651444953 35.0 31.0 37.0 18.0 39.0 76-77 30.986071052655255 35.0 31.0 36.0 15.0 39.0 78-79 30.597392276989602 34.5 31.0 36.0 10.0 38.0 80-81 30.14580128406968 34.0 30.0 35.0 7.0 37.0 82-83 29.696224259380877 34.0 30.0 35.0 3.5 37.0 84-85 29.292812268533098 34.0 29.0 35.0 2.0 36.0 86-87 28.888392393941302 34.0 29.0 35.0 2.0 36.0 88-89 28.418934480059733 34.0 29.0 35.0 2.0 35.5 90-91 27.959056709578945 34.0 27.5 35.0 2.0 35.0 92-93 27.453707368856662 33.5 26.5 35.0 2.0 35.0 94-95 26.910813683948533 33.0 25.0 35.0 2.0 35.0 96-97 26.24836852379967 33.0 24.5 35.0 2.0 35.0 98-99 25.381977293377837 32.5 21.0 35.0 2.0 35.0 100-101 22.16269075871665 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23798647774455972 1101 2 0.26420307450052505 1101 3 0.3047159842884746 1101 4 0.27800113257497827 1101 5 0.2516204325234668 1101 6 0.3153829147356575 1101 7 0.34281927803733225 1101 8 0.33386884928305705 1101 9 0.3472005774012814 1101 10-11 0.33740255260521934 1101 12-13 0.38061649126876773 1101 14-15 0.4905055373508418 1101 16-17 0.5012305441892906 1101 18-19 0.5734253568995769 1101 20-21 0.4336912343376369 1101 22-23 0.4732508592815634 1101 24-25 0.4994393674457882 1101 26-27 0.5137534288955479 1101 28-29 0.5146668595423947 1101 30-31 0.5332725619613967 1101 32-33 0.4464327709294835 1101 34-35 0.31281186847431286 1101 36-37 0.35823099616965237 1101 38-39 0.573796063744382 1101 40-41 0.5560798540472831 1101 42-43 0.42688412953934574 1101 44-45 0.5840931537822556 1101 46-47 0.49884427194235315 1101 48-49 0.46127142405901367 1101 50-51 0.3944864534013277 1101 52-53 0.46747839911594724 1101 54-55 0.44972112285889665 1101 56-57 0.49434724270734876 1101 58-59 0.5083168784228036 1101 60-61 0.5323935198013601 1101 62-63 0.4743209620404585 1101 64-65 0.46280126561384094 1101 66-67 0.4177311887236357 1101 68-69 0.25219818701911834 1101 70-71 0.18775151212287966 1101 72-73 0.13682771259702875 1101 74-75 -1.133381589859539E-4 1101 76-77 -0.04645643511079456 1101 78-79 0.10016369774445977 1101 80-81 -0.05626259475942064 1101 82-83 -0.06823236778696895 1101 84-85 -0.023112661040745053 1101 86-87 0.016238552266326423 1101 88-89 -0.11947463475410913 1101 90-91 -0.16228575521846622 1101 92-93 -0.15447735150891262 1101 94-95 -0.4637414084020506 1101 96-97 -0.6089487032298635 1101 98-99 -0.6909209401972696 1101 100-101 -0.2978343095435889 1102 1 0.23904055945762437 1102 2 0.24896530638985226 1102 3 0.2748958733757014 1102 4 0.078645818740398 1102 5 0.26868163962636515 1102 6 0.24307915437931626 1102 7 0.2863809665555479 1102 8 0.3321369778375569 1102 9 0.3301754487557389 1102 10-11 0.4335462613292762 1102 12-13 0.3324328675337398 1102 14-15 0.43751830965221217 1102 16-17 0.477817313248444 1102 18-19 0.4868001896015528 1102 20-21 0.4217163512735027 1102 22-23 0.48414628967065454 1102 24-25 0.46969167717913507 1102 26-27 0.44469382865539586 1102 28-29 0.43471045750980153 1102 30-31 0.4129314433119049 1102 32-33 0.4323802645368957 1102 34-35 0.3918732188361105 1102 36-37 0.4150700739072306 1102 38-39 0.5310802779107462 1102 40-41 0.4400853455682707 1102 42-43 0.24259885063651154 1102 44-45 0.4547216605529272 1102 46-47 0.39996493001341094 1102 48-49 0.4169614345776793 1102 50-51 0.32854510790062363 1102 52-53 0.26336431565803764 1102 54-55 0.37741493617355815 1102 56-57 0.31413176148350175 1102 58-59 0.18495515950162655 1102 60-61 0.3225977650120697 1102 62-63 0.3215608176380087 1102 64-65 0.18303171201301893 1102 66-67 0.17812001744073314 1102 68-69 0.21852506564918883 1102 70-71 0.1504001814765772 1102 72-73 0.08278872402177129 1102 74-75 0.13371853897255903 1102 76-77 0.08502981482999772 1102 78-79 0.18939120839103296 1102 80-81 0.16999717372026524 1102 82-83 0.08646124435336588 1102 84-85 -0.020236142514825417 1102 86-87 -0.038119445826581 1102 88-89 -0.10468626497186406 1102 90-91 -0.2221062110919263 1102 92-93 -0.0328947436039968 1102 94-95 -0.14116529261509925 1102 96-97 -0.27623448818052765 1102 98-99 -0.6142238364640029 1102 100-101 -0.3535565374917127 1103 1 0.2756949094041481 1103 2 0.20373841462270903 1103 3 0.36921203390623347 1103 4 0.32669117355869304 1103 5 0.08778913504355046 1103 6 0.24851786235276307 1103 7 0.15869930800850085 1103 8 0.14697457233766187 1103 9 0.3312753240195292 1103 10-11 0.37461491331524854 1103 12-13 0.36967407912244 1103 14-15 0.45886776193733425 1103 16-17 0.4408804893932299 1103 18-19 0.4571150112444684 1103 20-21 0.4915666939712082 1103 22-23 0.48517346752043267 1103 24-25 0.6052083387465217 1103 26-27 0.5515346553139082 1103 28-29 0.478421895846445 1103 30-31 0.42430424768002695 1103 32-33 0.44401043867073753 1103 34-35 0.4401172624859413 1103 36-37 0.39490595557257535 1103 38-39 0.6297966717006034 1103 40-41 0.5130700974164242 1103 42-43 0.37498973260258595 1103 44-45 0.41435540156708583 1103 46-47 0.4195553140806467 1103 48-49 0.38490961544256663 1103 50-51 0.3555525633080805 1103 52-53 0.3625929908653518 1103 54-55 0.5127303461948145 1103 56-57 0.4191085532263301 1103 58-59 0.4249855982749793 1103 60-61 0.4494036550716203 1103 62-63 0.36087265031889615 1103 64-65 0.2603032054741732 1103 66-67 0.1958627365855179 1103 68-69 0.11948310250520677 1103 70-71 0.16940323319895612 1103 72-73 0.18077249523934924 1103 74-75 0.16078729032909678 1103 76-77 0.31623494444320954 1103 78-79 0.19859522400790652 1103 80-81 0.193658717572756 1103 82-83 0.32117415494339596 1103 84-85 0.2183373145610119 1103 86-87 0.22641755271162722 1103 88-89 0.11737849848696413 1103 90-91 0.1905785985480648 1103 92-93 0.04577201720320545 1103 94-95 0.18107765795850383 1103 96-97 0.32245303584491936 1103 98-99 0.22619414751641287 1103 100-101 0.30019143260297554 1104 1 0.21603234925813908 1104 2 0.281903131729063 1104 3 0.22605509430112036 1104 4 0.21044573635942498 1104 5 0.1672654499597641 1104 6 0.25914918805376885 1104 7 0.3011972340081712 1104 8 0.3039079554470021 1104 9 0.3126342090369505 1104 10-11 0.3195360494526298 1104 12-13 0.3385998506505601 1104 14-15 0.37119142005912664 1104 16-17 0.2871208452916889 1104 18-19 0.4197150299104635 1104 20-21 0.2832501081905576 1104 22-23 0.3200436350996583 1104 24-25 0.40977767257761144 1104 26-27 0.3347517927786541 1104 28-29 0.3205509608870969 1104 30-31 0.3724693795864482 1104 32-33 0.4403982041427881 1104 34-35 0.43715419006184675 1104 36-37 0.43153565318332454 1104 38-39 0.5141054073900051 1104 40-41 0.4309733349309468 1104 42-43 0.40006102636399277 1104 44-45 0.49308927621450493 1104 46-47 0.5046787689504058 1104 48-49 0.4292954520943084 1104 50-51 0.31357386329148795 1104 52-53 0.42082454263239555 1104 54-55 0.5740164338005869 1104 56-57 0.5365282375634806 1104 58-59 0.5100927722421318 1104 60-61 0.4356158564155663 1104 62-63 0.39990279591372513 1104 64-65 0.3672447808848247 1104 66-67 0.40549294232634736 1104 68-69 0.39111715551665327 1104 70-71 0.39802521552847026 1104 72-73 0.48140345799030726 1104 74-75 0.39574788432279107 1104 76-77 0.45293660986547835 1104 78-79 0.3743480342783556 1104 80-81 0.3379706161126599 1104 82-83 0.46307870944812635 1104 84-85 0.4987599439786301 1104 86-87 0.33299602251418037 1104 88-89 0.3742966376938668 1104 90-91 0.38227782701994073 1104 92-93 0.3332300705464917 1104 94-95 0.47096498789047914 1104 96-97 0.29150324739295286 1104 98-99 0.417769869268529 1104 100-101 0.31594165037300925 1105 1 0.1739093298541725 1105 2 0.0976785036965957 1105 3 0.16120338477582763 1105 4 0.17979237800580705 1105 5 0.20880414189247176 1105 6 0.23959983919557715 1105 7 0.212841041328673 1105 8 0.2502693947595702 1105 9 0.25850109518723485 1105 10-11 0.2925128404103745 1105 12-13 0.2055097355169977 1105 14-15 0.39268844439828854 1105 16-17 0.3656224207116381 1105 18-19 0.4325634316894309 1105 20-21 0.40198124962719817 1105 22-23 0.23641171612216283 1105 24-25 0.4229332832335828 1105 26-27 0.23179858545243093 1105 28-29 0.25899011535610583 1105 30-31 0.3268479427526074 1105 32-33 0.2798457405927408 1105 34-35 0.42502447929402365 1105 36-37 0.4113372777805395 1105 38-39 0.505182198586084 1105 40-41 0.4181967327375915 1105 42-43 0.4042911937891063 1105 44-45 0.4542167521581675 1105 46-47 0.5302292423708508 1105 48-49 0.5798944870602014 1105 50-51 0.4267563267938641 1105 52-53 0.37473630918423595 1105 54-55 0.4832123022756676 1105 56-57 0.39030401192647446 1105 58-59 0.3703239549908375 1105 60-61 0.6272429422779453 1105 62-63 0.6084958358624917 1105 64-65 0.6285846465570373 1105 66-67 0.5726461513209387 1105 68-69 0.3957789149183242 1105 70-71 0.35180063238316706 1105 72-73 0.3531405491596864 1105 74-75 0.31038959743905536 1105 76-77 0.24966160533703885 1105 78-79 0.39200502213578403 1105 80-81 0.453622915844452 1105 82-83 0.46617890234372084 1105 84-85 0.48495222823428463 1105 86-87 0.45663178073369437 1105 88-89 0.6372796512823626 1105 90-91 0.6315165329351942 1105 92-93 0.6542118062413955 1105 94-95 0.5308399334697675 1105 96-97 0.5154527092237764 1105 98-99 0.4590069632185383 1105 100-101 0.6816894842698851 1106 1 0.21829458600035778 1106 2 0.26061489387013026 1106 3 0.31971246002946074 1106 4 0.3028688606967691 1106 5 0.333682281832381 1106 6 0.3024428196779567 1106 7 0.25896227977440134 1106 8 0.2099503370476583 1106 9 0.2682685685292867 1106 10-11 0.22777964680702922 1106 12-13 0.260133917320573 1106 14-15 0.30092460002800436 1106 16-17 0.3585032466229592 1106 18-19 0.3960567760694289 1106 20-21 0.32312200626580534 1106 22-23 0.43721373554069487 1106 24-25 0.35730873887748515 1106 26-27 0.3396675357819703 1106 28-29 0.25082205679856173 1106 30-31 0.2910721842189048 1106 32-33 0.42262600054107935 1106 34-35 0.37553117684642245 1106 36-37 0.2780352246150315 1106 38-39 0.43068770661771794 1106 40-41 0.50651589729096 1106 42-43 0.3999225650849212 1106 44-45 0.3886937359126179 1106 46-47 0.45469610969395546 1106 48-49 0.48211889568169397 1106 50-51 0.31986622999224323 1106 52-53 0.3513061487008855 1106 54-55 0.30437228271869543 1106 56-57 0.36536299253063476 1106 58-59 0.5693290560962794 1106 60-61 0.7654500812598215 1106 62-63 0.5894557968328087 1106 64-65 0.36251474754040913 1106 66-67 0.40997408845833405 1106 68-69 0.4068108225085041 1106 70-71 0.32493996102243017 1106 72-73 0.3157934265122968 1106 74-75 0.35567616089010556 1106 76-77 0.0846805318809487 1106 78-79 0.2378483961891824 1106 80-81 0.4396185945093656 1106 82-83 0.5776650489024426 1106 84-85 0.6390166124698844 1106 86-87 0.5571863989798622 1106 88-89 0.604755903708913 1106 90-91 0.6356736327753794 1106 92-93 0.7153047312207832 1106 94-95 0.7334375308963068 1106 96-97 0.6626740700872347 1106 98-99 0.7271345177562658 1106 100-101 1.0254004329253803 1107 1 0.10195507200577225 1107 2 0.16944979673652583 1107 3 0.2010006517924161 1107 4 0.18313426298959 1107 5 0.26819563910859046 1107 6 0.19902599243405916 1107 7 0.1626830478459027 1107 8 0.17024693006123925 1107 9 0.29834849475123804 1107 10-11 0.23906613282743905 1107 12-13 0.3183021279029248 1107 14-15 0.38116450990481354 1107 16-17 0.3084109771981147 1107 18-19 0.29760281612250594 1107 20-21 0.35602807608502474 1107 22-23 0.3250616789025429 1107 24-25 0.3274745989091272 1107 26-27 0.37581311059845746 1107 28-29 0.3588893755212439 1107 30-31 0.3404322964605129 1107 32-33 0.3332685687520396 1107 34-35 0.38694653077862995 1107 36-37 0.489570589718916 1107 38-39 0.6233637448713409 1107 40-41 0.41250099172537347 1107 42-43 0.5074084317895782 1107 44-45 0.2634204422174875 1107 46-47 0.3729597856253619 1107 48-49 0.3517592089385033 1107 50-51 0.3010505307877551 1107 52-53 0.41256250306047093 1107 54-55 0.4673340047313985 1107 56-57 0.2669032686734809 1107 58-59 0.3213880372993714 1107 60-61 0.2480621916569845 1107 62-63 0.19767143080670024 1107 64-65 0.2857910103522201 1107 66-67 0.2815537934960872 1107 68-69 0.3835379805539887 1107 70-71 0.2910495746623738 1107 72-73 0.40794729198731616 1107 74-75 0.43028932284941845 1107 76-77 0.43552356232939005 1107 78-79 0.3664204272599356 1107 80-81 0.21461400212560378 1107 82-83 0.2801985451872966 1107 84-85 0.22034950968296485 1107 86-87 0.4020781218173184 1107 88-89 0.2413215879178594 1107 90-91 0.4305481107431852 1107 92-93 0.4280992529755636 1107 94-95 0.5633235929178504 1107 96-97 0.6741298651132155 1107 98-99 0.5809807328822707 1107 100-101 0.646685335042001 1108 1 0.20255667732163474 1108 2 0.19589306729590206 1108 3 0.2402819857260603 1108 4 0.15688932811821843 1108 5 0.1862694153269402 1108 6 0.22562218718340432 1108 7 0.3552720466194046 1108 8 0.304525818760375 1108 9 0.3518494495570934 1108 10-11 0.3659708928839578 1108 12-13 0.4657073653623627 1108 14-15 0.5535091016670961 1108 16-17 0.537377205792609 1108 18-19 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-1.3588737562568092 2316 86-87 -1.388866878849747 2316 88-89 -1.5261642521568533 2316 90-91 -1.6586631329045574 2316 92-93 -1.6200694320616087 2316 94-95 -1.6682079379307702 2316 96-97 -1.834814917242369 2316 98-99 -1.7901787284947908 2316 100-101 -1.5970341923136395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9325.0 3 2580.0 4 2707.0 5 2971.0 6 3591.0 7 4453.0 8 5226.0 9 6448.0 10 8324.0 11 10551.0 12 14070.0 13 19633.0 14 17106.0 15 16725.0 16 20940.0 17 26225.0 18 23793.0 19 18744.0 20 19609.0 21 21867.0 22 23942.0 23 26974.0 24 30512.0 25 35858.0 26 41298.0 27 48820.0 28 57374.0 29 68076.0 30 82115.0 31 100738.0 32 124817.0 33 160796.0 34 216595.0 35 310508.0 36 474072.0 37 681435.0 38 526495.0 39 69384.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.047793470592396 18.543842521627553 13.892267632604618 23.516096375175437 2 15.494039945794496 20.473052571234764 39.2470957585544 24.785811724416337 3 20.25274317901091 23.767884506572972 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25.668987426777505 27.972685111194647 23.255156807000247 24-25 23.44763642246729 25.871935876690376 27.84289200832878 22.837535692513555 26-27 23.016163151424347 25.610965443054994 28.617845257304214 22.755026148216448 28-29 22.596872294096197 26.718504967983108 28.05925263405901 22.625370103861687 30-31 23.066573690717515 25.896757981473538 27.629327879624654 23.407340448184293 32-33 22.97369075723961 26.053115435549994 27.925815803791167 23.047378003419233 34-35 23.622827291126256 25.98490244197774 27.6999651779946 22.692305088901406 36-37 23.37302488435876 25.623208986632072 28.206592963473238 22.797173165535934 38-39 22.914881157999385 26.34222922775184 28.031897288477385 22.71099232577139 40-41 23.121521276424293 26.400063622995194 27.590690256779936 22.887724843800576 42-43 22.7933359529245 26.32139189485636 27.26774431504521 23.617527837173927 44-45 23.092851234003312 25.889403046471575 27.470620941968164 23.547124777556945 46-47 23.673058390372876 26.44176903851126 27.188365801224545 22.696806769891317 48-49 23.409052652295987 26.318628152022544 27.20958609381054 23.062733101870933 50-51 22.525013577464485 26.619440397492706 27.817632801466424 23.037913223576385 52-53 23.216048264623616 26.962415473467626 26.656150421287744 23.165385840621017 54-55 22.825939062300588 26.42284575328475 27.043219264185662 23.707995920228996 56-57 23.162306482262338 26.331955513253796 27.05968735867402 23.44605064580985 58-59 23.70619991968774 26.155543097348037 26.960925042995378 23.177331939968845 60-61 23.217438411155207 26.432214855641007 27.60609999900628 22.74424673419751 62-63 22.803135906350704 26.477884911501675 27.59849502530288 23.120484156844736 64-65 22.612450943662935 26.96331591150826 27.180292531139717 23.243940613689087 66-67 22.934579766036144 26.4085592826692 26.760252610307518 23.89660834098714 68-69 22.57647376071124 26.365451868171156 27.308359067140632 23.74971530397697 70-71 23.38659025331893 26.128840054847107 26.939111542596716 23.545458149237245 72-73 23.00456761531055 25.957534606012096 27.52446764531722 23.51343013336014 74-75 22.417147532726446 27.13162183347684 26.95554249031322 23.49568814348349 76-77 22.80373623363409 26.199968448350447 26.914446059863455 24.081849258152012 78-79 22.823980504947883 26.27960383200221 27.19855643092068 23.697859232129233 80-81 23.138113236655354 26.161750809935203 26.906014371528848 23.794121581880592 82-83 22.97288582902695 25.92144155698183 27.517317192015845 23.58835542197538 84-85 22.709238562955164 26.26160594904427 27.214038847666483 23.815116640334082 86-87 22.498808938700346 26.342810494839636 27.27461067136683 23.88376989509319 88-89 22.56626193769645 26.233654886431783 26.8960181058202 24.304065070051564 90-91 22.768773659076018 25.713526613074468 27.071928112124976 24.44577161572454 92-93 23.03571189049501 25.997216981267147 27.08961136453069 23.87745976370715 94-95 22.811124536931043 25.689126638271986 27.314839865069253 24.184908959727718 96-97 22.210351511194766 25.654284404854643 27.830362063719345 24.30500202023125 98-99 22.537573851194846 25.97342057800499 27.06479282329382 24.424212747506342 100-101 22.68351422859751 24.893901616341914 27.679800690993737 24.742783464066832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2522.0 1 1482.5 2 519.0 3 589.0 4 526.5 5 557.0 6 890.5 7 1670.0 8 2735.0 9 3259.5 10 3073.0 11 2939.5 12 3128.5 13 3484.5 14 3890.5 15 4319.0 16 4692.5 17 4936.5 18 5218.0 19 5437.5 20 5706.0 21 6276.5 22 7226.0 23 8664.5 24 10276.5 25 12193.0 26 15300.5 27 18946.0 28 22015.5 29 25576.5 30 29290.5 31 33809.5 32 39210.0 33 44862.5 34 51105.0 35 56752.0 36 63922.0 37 70903.0 38 75732.0 39 80025.0 40 83209.0 41 86041.5 42 89360.5 43 94956.5 44 102046.5 45 107486.0 46 114658.5 47 134615.5 48 166405.0 49 166783.0 50 139773.5 51 127299.0 52 124823.0 53 125297.5 54 124451.0 55 118828.5 56 109734.5 57 99440.0 58 91945.0 59 84554.0 60 72550.5 61 59389.0 62 47556.0 63 36879.0 64 27799.0 65 20075.0 66 13852.0 67 9712.5 68 7039.5 69 5140.5 70 3660.5 71 2329.0 72 1528.0 73 1022.0 74 666.5 75 398.0 76 262.0 77 177.0 78 113.5 79 85.0 80 62.0 81 44.5 82 41.0 83 35.5 84 28.0 85 22.0 86 20.5 87 18.0 88 17.0 89 14.5 90 13.5 91 12.5 92 7.0 93 7.5 94 5.5 95 4.0 96 5.5 97 2.5 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32461593361941515 2 0.0883135265135499 3 0.09790956335033632 4 0.10267759415361456 5 0.11359308605545909 6 0.05655664210680989 7 0.0588057132404317 8 0.05931550269738598 9 0.06273409082049114 10-11 0.06018514353571975 12-13 0.05826593616836247 14-15 0.05681153683528702 16-17 0.058490843281724644 18-19 0.0670673012046025 20-21 0.0686866324208102 22-23 0.08478998173754239 24-25 0.0938312476947021 26-27 0.08798366274728536 28-29 0.08621439345550287 30-31 0.07993198808891928 32-33 0.07146048681894378 34-35 0.3197429461632346 36-37 0.49723964002866816 38-39 0.4721400061774487 40-41 0.45447730087473875 42-43 0.483580281343805 44-45 0.4931463305654764 46-47 0.48735872084828963 48-49 0.504406680041143 50-51 0.5011530238011701 52-53 0.4998635563512269 54-55 0.4913920550812514 56-57 0.4707755696897182 58-59 0.4548221584485607 60-61 0.4155383826479664 62-63 0.48328040519265547 64-65 0.5050214261509997 66-67 0.5154571162110049 68-69 0.527047329452936 70-71 0.5244384069379346 72-73 0.5132230388849406 74-75 0.5149323329464931 76-77 0.4900426124010784 78-79 0.47122538391644253 80-81 0.47758275832081354 82-83 0.4854095258658174 84-85 0.4876286093843243 86-87 0.4870288570820251 88-89 0.4929963924899017 90-91 0.5060859864875806 92-93 0.5124733485070666 94-95 0.5189206857567824 96-97 0.5104042030641345 98-99 0.5140926797232743 100-101 0.49316132437303395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3334710.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.81494351682087 #Duplication Level Percentage of deduplicated Percentage of total 1 68.90243610828668 29.50053910147653 2 14.399689549814983 12.330437894701687 3 6.031098442297921 7.746634174541156 4 3.11656759456707 5.337426621109736 5 1.8450287138061017 3.949740008426045 6 1.1749371607124375 3.0182920903030137 7 0.837137614701336 2.50893997824707 8 0.6050713164000505 2.072487538825329 9 0.4588925007241283 1.7682710848916607 >10 2.4086125805205367 18.453991604182658 >50 0.15519264144300204 4.525480865986613 >100 0.06203678659901069 4.401076652216524 >500 0.0012634430276389297 0.36627812210108296 >1k 0.001474016864901097 1.7223606601923822 >5k 4.211476756860277E-4 1.3340541608493865 >10k+ 1.403825585620092E-4 0.9639894419491738 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21155 0.6343879977569263 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10922 0.32752473228556606 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9019 0.27045830072180194 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8891 0.2666198859870873 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7980 0.239301168617361 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7000 0.20991330580470263 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6325 0.18967166560210633 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5176 0.15521589583502013 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4947 0.14834873197369486 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4736 0.14202134518443882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4451 0.13347487487667592 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4342 0.13020622482914557 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4154 0.12456855318753356 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4014 0.1203702870714395 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3990092091966017E-4 2 0.0 0.0 0.0 0.0 2.3990092091966017E-4 3 0.0 0.0 0.0 0.0 2.3990092091966017E-4 4 0.0 0.0 0.0 0.0 2.3990092091966017E-4 5 0.0 0.0 0.0 0.0 2.3990092091966017E-4 6 0.0 0.0 0.0 0.0 2.9987615114957525E-4 7 0.0 0.0 0.0 0.0 2.9987615114957525E-4 8 0.0 0.0 0.0 0.0 2.9987615114957525E-4 9 0.0 0.0 0.0 0.0 2.9987615114957525E-4 10-11 0.0 0.0 0.0 1.499380755747876E-5 2.9987615114957525E-4 12-13 0.0 0.0 0.0 2.998761511495752E-5 6.447337249715868E-4 14-15 0.0 0.0 0.0 4.4981422672436285E-5 8.246594156613319E-4 16-17 0.0 0.0 0.0 1.1995046045983008E-4 9.296160685636832E-4 18-19 0.0 0.0 0.0 1.6493188313226637E-4 0.0010945479516959494 20-21 0.0 0.0 0.0 1.949194982472239E-4 0.0011545231819258647 22-23 0.0 0.0 0.0 3.448575738220115E-4 0.0012144984121557796 24-25 0.0 0.0 0.0 6.747213400865443E-4 0.0012294922197132584 26-27 0.0 0.0 0.0 0.001184510797040822 0.001319455065058131 28-29 0.0 0.0 0.0 0.004018340425404308 0.001379430295288046 30-31 0.0 0.0 0.0 0.012324909812247542 0.001379430295288046 32-33 0.0 0.0 0.0 0.029327887582428457 0.0014993807557478762 34-35 0.0 0.0 0.0 0.0530181035232449 0.0015743497935352699 36-37 0.0 0.0 0.0 0.09242182978429908 0.0015893436010927487 38-39 0.0 0.0 0.0 0.15206719624794962 0.0016643126388801426 40-41 0.0 0.0 0.0 0.21865469561071277 0.0017092940615525789 42-43 0.0 0.0 0.0 0.28948544251224245 0.0017542754842250151 44-45 0.0 0.0 0.0 0.379073442668178 0.0018592321371273665 46-47 0.0 0.0 0.0 0.4882433554941809 0.0019192073673572813 48-49 0.0 0.0 0.0 0.6013266520926857 0.0020391578278171115 50-51 0.0 0.0 0.0 0.7259101990877768 0.002129120673161984 52-53 0.0 0.0 0.0 0.8508685912718048 0.002339033978966687 54-55 0.0 0.0 0.0 0.9919603203876799 0.0024289968243115592 56-57 0.0 0.0 0.0 1.1712412773524534 0.0025039658620989533 58-59 0.0 0.0 0.0 1.357194478680305 0.002563941092328868 60-61 0.0 0.0 0.0 1.565803323227507 0.002623916322558783 62-63 0.0 0.0 0.0 1.7837083284603459 0.00290879866615088 64-65 0.0 0.0 0.0 2.0159774013332497 0.002938786281265837 66-67 0.0 0.0 0.0 2.2837518105022623 0.002938786281265837 68-69 0.0 0.0 0.0 2.5947383730519293 0.0029687738963807947 70-71 0.0 0.0 0.0 2.925636712037928 0.0032086748173004546 72-73 0.0 0.0 0.0 3.257779537051198 0.0035985138137949024 74-75 0.0 0.0 0.0 3.6239133237972716 0.003703470466697254 76-77 0.0 0.0 0.0 4.007394945887349 0.0038084271195996055 78-79 0.0 0.0 0.0 4.465470760575882 0.0038534085422720415 80-81 0.0 0.0 0.0 4.9624405120685156 0.003898389964944478 82-83 0.0 0.0 0.0 5.452513112084709 0.004198266116094053 84-85 0.0 0.0 0.0 5.958689061417634 0.004198266116094053 86-87 0.0 0.0 0.0 6.48395212777123 0.0043182165765538825 88-89 0.0 0.0 0.0 7.076222520099199 0.004438167037013713 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10615 0.0 24.146908 1 TACATGG 10530 0.0 23.839054 2 ACATGGG 11120 0.0 21.892727 3 GAGTACT 8650 0.0 20.715672 12-13 AGAGTAC 13740 0.0 19.966995 10-11 TTTGCGT 960 0.0 19.73469 3 AGTACTT 9010 0.0 19.152346 12-13 CATGGGG 7830 0.0 19.114672 4 GTACTTT 9230 0.0 19.055744 14-15 GTATACG 180 1.240892E-4 18.459217 1 GTATAGG 920 0.0 18.05793 1 GTATAGA 880 0.0 17.260567 1 CCACGCG 220 3.0046325E-5 17.21525 9 AGTACAT 7985 0.0 16.252388 2 GAGTACA 8610 0.0 16.097834 1 ACTTTTT 11155 0.0 16.00256 16-17 TACTTTT 10765 0.0 15.854774 14-15 CTTTGCG 1265 0.0 15.725348 2 ACCGTCC 605 0.0 14.867716 8 CATATAG 610 0.0 14.752488 3 >>END_MODULE