FastQCFastQC Report
Fri 27 May 2016
SRR939031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334710
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT220970.6626363311952164No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT105340.31588953762096256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91190.27345706223329763No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT84620.25375519910277056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75890.22757601110741263No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC75730.2270962092655733No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT61290.18379409303957464No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC53370.16004390186852832No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC53200.159534112411574No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT52540.1575549298139868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.14786893013185554No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG47840.14346075070995679No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA46530.13953237312989736No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC40450.12129990314000319No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35750.10720572403597314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104300.022.3808441
TACATGG104050.021.9373632
GAGTACT93600.021.21910512-13
TTTGCGT7850.020.553733
AGAGTAC145800.020.37594410-11
ACATGGG110700.019.8479083
GTACTTT100100.019.65189414-15
GTAAGCG6750.018.98311294-95
AGTACTT99550.018.7828912-13
CATGGGG77200.016.8428134
CGTACAC2858.6801083E-716.6508563
GTATAGG8400.016.4468751
ACTTTTT119750.016.34742516-17
GAGTACA87400.016.0796011
TACTTTT118000.015.68543414-15
AGTACAT81900.015.5285982
TATACAG18500.014.8777645
GTATAGA8100.014.70346451
CCCTATA7450.014.6505172
CGATACG1000.005061599414.34163486-87
Produced by FastQC (version 0.11.5)