##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939031_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3334710 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16945011710164 33.0 31.0 34.0 30.0 34.0 2 32.40743303015854 34.0 31.0 34.0 31.0 34.0 3 32.50267849378207 34.0 31.0 34.0 30.0 34.0 4 36.051880973158084 37.0 35.0 37.0 35.0 37.0 5 35.97312090106786 37.0 35.0 37.0 35.0 37.0 6 36.01492033790045 37.0 35.0 37.0 35.0 37.0 7 35.9464709075152 37.0 35.0 37.0 35.0 37.0 8 35.91656875710331 37.0 35.0 37.0 35.0 37.0 9 37.59278797856485 39.0 37.0 39.0 35.0 39.0 10-11 37.49894188700067 39.0 37.0 39.0 34.5 39.0 12-13 37.4128352990215 39.0 37.0 39.0 34.0 39.0 14-15 38.72054736393869 40.0 38.0 41.0 34.0 41.0 16-17 38.81752416252088 40.0 38.0 41.0 35.0 41.0 18-19 38.84626384303283 40.0 38.0 41.0 35.0 41.0 20-21 38.80529311394395 40.0 38.0 41.0 34.0 41.0 22-23 38.73071946286184 40.0 38.0 41.0 34.0 41.0 24-25 38.64888655985078 40.0 38.0 41.0 34.0 41.0 26-27 38.48508910819831 40.0 38.0 41.0 34.0 41.0 28-29 38.33449715267595 40.0 38.0 41.0 34.0 41.0 30-31 38.160778148624615 40.0 38.0 41.0 33.0 41.0 32-33 38.08244599980208 40.0 37.5 41.0 33.0 41.0 34-35 37.98366229747114 40.0 37.0 41.0 33.0 41.0 36-37 37.85728923954407 40.0 37.0 41.0 33.0 41.0 38-39 37.71910960773201 40.0 37.0 41.0 33.0 41.0 40-41 37.57355362235397 40.0 37.0 41.0 32.0 41.0 42-43 37.387118070236994 40.0 37.0 41.0 32.0 41.0 44-45 37.142608352750315 40.0 36.0 41.0 31.0 41.0 46-47 36.85394771959181 39.5 36.0 41.0 31.0 41.0 48-49 36.524785213706735 39.0 35.5 41.0 30.0 41.0 50-51 36.25556420198458 39.0 35.0 40.5 30.0 41.0 52-53 36.00253695223873 39.0 35.0 40.0 29.0 41.0 54-55 35.69480089722944 38.0 35.0 40.0 28.0 41.0 56-57 35.527019141094726 38.0 35.0 40.0 28.0 41.0 58-59 35.22375813788905 38.0 34.0 40.0 28.0 41.0 60-61 34.81769914025507 37.5 34.0 40.0 27.0 41.0 62-63 34.386701842139196 37.0 34.0 40.0 26.0 41.0 64-65 33.96383628561404 36.0 33.0 39.0 26.0 40.5 66-67 33.48376395548638 36.0 33.0 39.0 25.5 40.0 68-69 32.966227647981384 35.0 32.0 38.5 24.5 40.0 70-71 32.41849786038366 35.0 31.5 37.5 23.0 39.5 72-73 31.890833835625884 35.0 31.0 37.0 21.5 39.0 74-75 31.373765634792832 34.5 31.0 36.0 20.0 39.0 76-77 29.50424999475217 32.5 28.5 34.5 18.0 36.5 78-79 30.48545540691694 34.0 30.0 35.0 19.0 37.0 80-81 30.47322960617265 34.0 30.0 35.0 18.0 37.0 82-83 30.066082957738455 34.0 30.0 35.0 16.5 36.5 84-85 29.61452854970897 34.0 29.5 35.0 9.0 36.0 86-87 28.940859025222583 34.0 29.0 35.0 4.5 35.5 88-89 28.253108216306664 33.0 27.5 35.0 2.0 35.0 90-91 27.661633245469623 33.0 26.5 35.0 2.0 35.0 92-93 27.23853438529887 33.0 26.0 34.0 2.0 35.0 94-95 26.660771551349292 32.0 25.0 34.0 2.0 35.0 96-97 25.7700734096818 32.0 23.5 34.0 2.0 35.0 98-99 24.509516269780583 32.0 10.5 34.0 2.0 35.0 100-101 18.901767170158728 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14673581482400522 1101 2 0.1761330407433448 1101 3 0.18678741190544912 1101 4 0.17373436155983768 1101 5 0.1471838292237564 1101 6 0.1329420043239935 1101 7 0.163594278845828 1101 8 0.1657671265793752 1101 9 0.20832582075902906 1101 10-11 0.3048343089407908 1101 12-13 0.25821279863123436 1101 14-15 0.30045994518928154 1101 16-17 0.2282000989609685 1101 18-19 0.2459232123982602 1101 20-21 0.2648350990257029 1101 22-23 0.22527954138766404 1101 24-25 0.1873881997075273 1101 26-27 0.12027367983350246 1101 28-29 0.2051344576129992 1101 30-31 0.17324175333566671 1101 32-33 0.2581802487618745 1101 34-35 0.33582998939995434 1101 36-37 0.4928482329349677 1101 38-39 0.42648560624220266 1101 40-41 0.44630652096954293 1101 42-43 0.4032245672915735 1101 44-45 0.40251799928051213 1101 46-47 0.40865869743627314 1101 48-49 0.41867909309797824 1101 50-51 0.3398158627325145 1101 52-53 0.2909127770977875 1101 54-55 0.3321698296423321 1101 56-57 0.20049667190519216 1101 58-59 0.2959330717277453 1101 60-61 0.3375386281459143 1101 62-63 0.33201382531281354 1101 64-65 0.3020287578182561 1101 66-67 0.05424337776327803 1101 68-69 0.10570718580841998 1101 70-71 0.2695716811674487 1101 72-73 0.29389730245023316 1101 74-75 0.3500423444408547 1101 76-77 0.06633453381869003 1101 78-79 0.3869382946325395 1101 80-81 0.48111692433798225 1101 82-83 0.44141774315582083 1101 84-85 0.5301890456304648 1101 86-87 0.7576997580416212 1101 88-89 0.8075240039356437 1101 90-91 0.6548883987373557 1101 92-93 0.7573189997346326 1101 94-95 0.8180230885024287 1101 96-97 1.0436744598238015 1101 98-99 1.1915774856628616 1101 100-101 1.51496649371148 1102 1 0.16860990923625963 1102 2 0.1729507520167317 1102 3 0.1432973633883634 1102 4 0.10641164366204237 1102 5 0.1381431163725182 1102 6 0.08569262274778566 1102 7 0.14763557454349296 1102 8 0.18473732714500812 1102 9 0.13419524564837104 1102 10-11 0.20765534892658621 1102 12-13 0.20926166373693889 1102 14-15 0.24366364468695423 1102 16-17 0.27291011625014505 1102 18-19 0.2459467372403381 1102 20-21 0.2323829486072313 1102 22-23 0.20626556140741314 1102 24-25 0.2892550910874263 1102 26-27 0.339876392722509 1102 28-29 0.3156566447565936 1102 30-31 0.2831915378196328 1102 32-33 0.33883369292007615 1102 34-35 0.3818644131009279 1102 36-37 0.34454551864290295 1102 38-39 0.4240921381730587 1102 40-41 0.41316017672472327 1102 42-43 0.5184053061214158 1102 44-45 0.4544702394204094 1102 46-47 0.4082338788755777 1102 48-49 0.3799344693947049 1102 50-51 0.39259521733111313 1102 52-53 0.3506853920928634 1102 54-55 0.40115584881814215 1102 56-57 0.3805402002475091 1102 58-59 0.2562024617623493 1102 60-61 0.3787429692493731 1102 62-63 0.3766414529084514 1102 64-65 0.302641669622993 1102 66-67 0.10431436898513624 1102 68-69 0.32002720024825493 1102 70-71 0.35889846031802364 1102 72-73 0.39778421821790744 1102 74-75 0.4156187384117018 1102 76-77 0.13231522804627716 1102 78-79 0.46959266902417696 1102 80-81 0.5861225360149973 1102 82-83 0.5017546906292765 1102 84-85 0.6744507089676084 1102 86-87 0.8174807596956377 1102 88-89 0.6057679430449134 1102 90-91 0.7065340774823241 1102 92-93 0.6545159673297398 1102 94-95 0.5442000453183091 1102 96-97 0.6939163911985666 1102 98-99 0.8339940840318825 1102 100-101 0.8382011468376831 1103 1 0.025869530529803342 1103 2 0.06213175550254846 1103 3 0.017261804415852566 1103 4 0.0533443096435775 1103 5 0.07156975343721683 1103 6 0.033986142479378145 1103 7 0.044147092376590535 1103 8 0.03483724686405054 1103 9 0.06411839036068301 1103 10-11 0.0737754251804219 1103 12-13 0.0661590531675671 1103 14-15 0.12100155952638403 1103 16-17 0.04935505580407096 1103 18-19 0.10754869536349076 1103 20-21 0.15327283817514825 1103 22-23 0.11028238118320388 1103 24-25 0.11774217422136246 1103 26-27 0.1713054722111167 1103 28-29 0.08979001574614642 1103 30-31 0.09620479333012355 1103 32-33 0.21838914038835355 1103 34-35 0.28280639376551164 1103 36-37 0.14666990444128203 1103 38-39 0.24510634554896882 1103 40-41 0.2757246695295166 1103 42-43 0.39398619245456246 1103 44-45 0.31572942139144544 1103 46-47 0.33030455226806055 1103 48-49 0.32695615048317705 1103 50-51 0.4112921031098722 1103 52-53 0.37455320832008 1103 54-55 0.47671408912231783 1103 56-57 0.528288750875511 1103 58-59 0.30665619299634983 1103 60-61 0.2943988542096392 1103 62-63 0.3162979968524695 1103 64-65 0.3217552841750333 1103 66-67 0.2155845078685772 1103 68-69 0.2589827633001818 1103 70-71 0.20916323057588926 1103 72-73 0.3087189552912335 1103 74-75 0.25604127251803277 1103 76-77 0.0930797014999527 1103 78-79 0.3835522984242665 1103 80-81 0.2774422555808016 1103 82-83 0.28149512425104817 1103 84-85 0.4005101691762363 1103 86-87 0.4782381726091032 1103 88-89 0.4373031398378586 1103 90-91 0.6203449125350282 1103 92-93 0.6199207324098772 1103 94-95 0.7276371162924278 1103 96-97 0.6465560656815121 1103 98-99 0.8807650374924627 1103 100-101 1.0698030788106863 1104 1 0.035131704911755435 1104 2 0.0970367662873528 1104 3 0.07320153396621265 1104 4 0.08134144752312267 1104 5 0.02576929300914088 1104 6 0.04896478027596629 1104 7 0.06090211145739488 1104 8 0.051056862759189414 1104 9 0.14909930955762718 1104 10-11 0.04639597644970905 1104 12-13 0.06993181532121184 1104 14-15 0.08028597756189271 1104 16-17 0.04930341319581544 1104 18-19 0.1382880246317839 1104 20-21 0.15735872252889038 1104 22-23 0.12412648894825651 1104 24-25 0.1560333016479447 1104 26-27 0.06798733093021525 1104 28-29 0.0638285091923052 1104 30-31 -0.12264978813660576 1104 32-33 0.15008546525976385 1104 34-35 0.16482408498833934 1104 36-37 0.11563010437490817 1104 38-39 0.1055784398745061 1104 40-41 0.17585040579962907 1104 42-43 0.12543934078706798 1104 44-45 0.11777734116911631 1104 46-47 0.17729227418609383 1104 48-49 -0.0712723421333763 1104 50-51 -0.028724644442810643 1104 52-53 -0.018810457268109815 1104 54-55 0.16934853619383006 1104 56-57 -0.06881288551075215 1104 58-59 0.17073932929964997 1104 60-61 0.0412220560513461 1104 62-63 -0.03592030205246033 1104 64-65 0.03840089973832761 1104 66-67 -0.04007341688669186 1104 68-69 -0.0130618380769576 1104 70-71 -0.10583023632306521 1104 72-73 -0.05835001558980224 1104 74-75 0.1507667267241075 1104 76-77 -0.049651031036965776 1104 78-79 0.169036525651741 1104 80-81 0.22734477756520377 1104 82-83 0.14702264623544892 1104 84-85 0.20575147911484493 1104 86-87 0.22573889850921702 1104 88-89 0.33245977871292 1104 90-91 0.5045284823044014 1104 92-93 0.4139863434103752 1104 94-95 0.5891302409813299 1104 96-97 0.5011276290629603 1104 98-99 0.6256483231237873 1104 100-101 0.8026205045614745 1105 1 5.805422755855716E-5 1105 2 0.04411401668298964 1105 3 0.019248167350177425 1105 4 0.04709870573627484 1105 5 -0.03652543763615057 1105 6 0.027141479949918335 1105 7 0.06002462717187029 1105 8 0.008726190611788809 1105 9 0.05243224036308902 1105 10-11 0.09306247958942748 1105 12-13 0.12443499992578921 1105 14-15 0.043432481686743074 1105 16-17 -0.037493050173090126 1105 18-19 -0.014650604300008752 1105 20-21 0.08501165266699928 1105 22-23 0.015497837199482944 1105 24-25 -0.08128160835529741 1105 26-27 -0.10404551891947023 1105 28-29 0.06078889409369026 1105 30-31 0.05785759758784792 1105 32-33 0.05499719248568624 1105 34-35 0.1603789656917911 1105 36-37 0.08198013122775905 1105 38-39 0.13575328328586522 1105 40-41 0.21317342925535598 1105 42-43 0.19849447571864687 1105 44-45 0.22506632983371588 1105 46-47 0.11530271660659963 1105 48-49 0.16776536069299652 1105 50-51 0.07307520135194068 1105 52-53 0.03063537505890679 1105 54-55 0.11525589029866268 1105 56-57 0.15507138070741178 1105 58-59 0.0762162672330362 1105 60-61 0.10050393506735844 1105 62-63 0.11727315085813217 1105 64-65 -0.05965141484991676 1105 66-67 0.06664167163236101 1105 68-69 0.16155162592433925 1105 70-71 0.27381826547878063 1105 72-73 0.18947747592532949 1105 74-75 0.11839112174060062 1105 76-77 -0.10267457812319236 1105 78-79 0.024473521962253386 1105 80-81 -0.020051370243031386 1105 82-83 -0.003261036608602552 1105 84-85 -0.02545540141064606 1105 86-87 0.007612452586720053 1105 88-89 0.21775418084780185 1105 90-91 0.12730487992355322 1105 92-93 0.24674301252928288 1105 94-95 0.38141369223943755 1105 96-97 0.4497242401225243 1105 98-99 0.6494839483502126 1105 100-101 0.7864951915430893 1106 1 0.07788315647608357 1106 2 0.027027998870146064 1106 3 0.03538381311826555 1106 4 0.019525470748149587 1106 5 0.1439358245310629 1106 6 0.07556287340193535 1106 7 0.10214543786880625 1106 8 0.10168056097810307 1106 9 0.06869942750213198 1106 10-11 0.16688782424170512 1106 12-13 0.12426171762488991 1106 14-15 0.04670311331413046 1106 16-17 -0.016739431086072898 1106 18-19 -0.012469449238487584 1106 20-21 0.0845280897378089 1106 22-23 0.11830469454497461 1106 24-25 0.16777373983059363 1106 26-27 0.031719249112491354 1106 28-29 0.01939385860289633 1106 30-31 0.07128515692195236 1106 32-33 0.12312193238273039 1106 34-35 -0.03459377968288635 1106 36-37 0.0810902643841942 1106 38-39 0.14668222672431597 1106 40-41 0.24323958384250943 1106 42-43 0.1954254642711959 1106 44-45 0.3131747314117206 1106 46-47 0.2931374594781673 1106 48-49 0.36489676315090946 1106 50-51 0.35789438892984293 1106 52-53 0.34573368212912214 1106 54-55 0.4197885244778021 1106 56-57 0.36135376403409225 1106 58-59 0.27011801310486305 1106 60-61 0.3550777022523448 1106 62-63 0.5027610740869903 1106 64-65 0.5295045787598909 1106 66-67 0.37495669464301074 1106 68-69 0.38421660623190945 1106 70-71 0.2993404028671378 1106 72-73 0.44020748745290916 1106 74-75 0.3900306106899265 1106 76-77 0.21046472945413797 1106 78-79 0.42591964413891503 1106 80-81 0.3841517595033821 1106 82-83 0.42515513539773053 1106 84-85 0.4613702133131028 1106 86-87 0.7491602732841507 1106 88-89 0.7823218467725468 1106 90-91 0.7797656848552634 1106 92-93 0.7383550482009333 1106 94-95 0.891102135449227 1106 96-97 0.8919521603433509 1106 98-99 1.0079405027915342 1106 100-101 1.0465939835165372 1107 1 0.027231319147688282 1107 2 0.06967240590905277 1107 3 0.02870489384675068 1107 4 -0.0034239830393474335 1107 5 0.019679509534775264 1107 6 0.057908906707027086 1107 7 0.029655222457890318 1107 8 0.09406488914047628 1107 9 0.0026956261330397524 1107 10-11 0.11228152274139802 1107 12-13 0.12269360601310098 1107 14-15 0.052942616436951084 1107 16-17 0.06606237287724781 1107 18-19 0.08337186609519875 1107 20-21 0.09389275687433951 1107 22-23 0.13988603971978364 1107 24-25 0.14429705227014722 1107 26-27 0.06774856164201282 1107 28-29 0.13502375558353918 1107 30-31 0.11122511853337613 1107 32-33 0.13452735177045838 1107 34-35 0.17245070270860197 1107 36-37 0.19000323777461148 1107 38-39 0.09672219920751246 1107 40-41 0.14061288091162538 1107 42-43 0.1761813144057811 1107 44-45 0.19684730757031588 1107 46-47 0.12953219454448828 1107 48-49 0.17600086593033382 1107 50-51 0.1446864120529625 1107 52-53 0.11170767986479291 1107 54-55 0.018126690748331953 1107 56-57 0.1358497264852616 1107 58-59 0.1532313962483869 1107 60-61 0.2110756834779366 1107 62-63 0.24980409441344165 1107 64-65 0.2644788614108009 1107 66-67 0.18041174364093848 1107 68-69 0.20394211289367803 1107 70-71 0.28584005984418326 1107 72-73 0.2875909065107436 1107 74-75 0.19189493105461963 1107 76-77 0.10352685597149858 1107 78-79 0.411936444765729 1107 80-81 0.25986095476779525 1107 82-83 0.06330406734047855 1107 84-85 0.2925935025095683 1107 86-87 0.42953898561723136 1107 88-89 0.672150700813468 1107 90-91 0.7442575141610419 1107 92-93 0.7040365860684155 1107 94-95 0.8755639455607174 1107 96-97 0.7617898055189123 1107 98-99 0.8205719480615663 1107 100-101 0.9666526723066227 1108 1 0.06430747849935159 1108 2 0.0034292564470064235 1108 3 0.07302981505586104 1108 4 0.04414339675602719 1108 5 0.10212757741138034 1108 6 0.07427160096732166 1108 7 0.10063940994139386 1108 8 0.13319756834123098 1108 9 0.11963023357586877 1108 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-0.5275416924896916 2316 54-55 -0.4612470433482869 2316 56-57 -0.4579902511984528 2316 58-59 -0.5864942438287528 2316 60-61 -0.4294699891033602 2316 62-63 -0.5590742848663695 2316 64-65 -0.40034115290072947 2316 66-67 -0.35729481843835487 2316 68-69 -0.3737070362979651 2316 70-71 -0.292936198175731 2316 72-73 -0.4388130334050828 2316 74-75 -0.6497698152795657 2316 76-77 -0.5064490691234056 2316 78-79 -0.6373398678320576 2316 80-81 -0.6390970627213832 2316 82-83 -0.5605041642152351 2316 84-85 -0.7848853182445765 2316 86-87 -0.6654544043962289 2316 88-89 -0.9198797930556175 2316 90-91 -0.9758420752920856 2316 92-93 -0.8948028003550341 2316 94-95 -0.8381857360683682 2316 96-97 -0.7811477422367261 2316 98-99 -0.7941890160261913 2316 100-101 -0.5428913295854123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 21.0 5 54.0 6 132.0 7 364.0 8 918.0 9 1805.0 10 2889.0 11 4486.0 12 6754.0 13 8908.0 14 10972.0 15 14219.0 16 18651.0 17 25312.0 18 25527.0 19 19597.0 20 19451.0 21 21391.0 22 23035.0 23 25860.0 24 29835.0 25 35601.0 26 42311.0 27 51211.0 28 62610.0 29 76054.0 30 91851.0 31 111912.0 32 137872.0 33 176381.0 34 238506.0 35 343670.0 36 537469.0 37 743680.0 38 407799.0 39 17591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91976705398678 19.26624534716109 14.433795717039958 23.38019188181217 2 15.792647636526114 21.072147203205077 38.49684080474764 24.63836435552117 3 20.174138080972558 23.96979047653319 29.827451262628536 26.02862017986571 4 13.47250585508185 16.493848040759165 36.599164545042896 33.43448155911609 5 13.865283637857564 37.34474661964609 34.16722893445007 14.622740808046277 6 32.484953714116074 35.85331258190367 16.58471051455749 15.07702318942277 7 28.46199519598406 32.47610736765716 20.817642313724434 18.24425512263435 8 26.11315526687478 34.47259281916569 19.562270782166966 19.85198113179257 9 25.830761895337222 17.1374722239715 20.660447235291823 36.37131864539945 10-11 25.47465896584711 25.853207625250775 26.91649348818938 21.755639920712746 12-13 26.52761409537861 23.30896839605243 27.308311667281416 22.85510584128755 14-15 23.071393774787786 24.805877262941003 26.18411983273365 25.93860912953756 16-17 22.448483561716344 27.850161914053977 26.63003002273559 23.07132450149409 18-19 22.38920729205901 27.312819519380476 28.109668767407886 22.188304421152633 20-21 23.44368775695638 25.863448395812526 27.862782670756975 22.830081176474117 22-23 23.014909842235156 25.732957288639792 28.069307376053686 23.182825493071363 24-25 23.329950727305278 25.98028852309963 27.95244079914667 22.73731995044842 26-27 22.946125134157132 25.701463006217335 28.702954290764204 22.64945756886133 28-29 22.488852104081015 26.829454435318212 28.146105658363098 22.535587802237675 30-31 23.007862752683142 25.931175424549664 27.748679795244563 23.31228202752263 32-33 22.902231378440703 26.09168413445247 28.043263132326352 22.962821354780473 34-35 23.572797673156877 26.001761087699588 27.837437089550647 22.588004149592884 36-37 23.296624585907423 25.698297798120034 28.373130762596222 22.631946853376324 38-39 22.85358679621725 26.392015229366468 28.127437227223616 22.62696074719267 40-41 23.059205135137283 26.43785088482843 27.68819707444842 22.81474690558586 42-43 22.77810924206571 26.385498648160517 27.359488839583264 23.476903270190512 44-45 23.085097131341726 25.905249710615173 27.54118860693444 23.46846455110866 46-47 23.616120138898154 26.46247076362948 27.29342167981363 22.62798741765873 48-49 23.387255059988142 26.366607264551334 27.238686296104426 23.0074513793561 50-51 22.557248446073444 26.74836323234716 27.77998604071284 22.914402280866554 52-53 23.250837404152087 27.044960431341856 26.601368634753847 23.10283352975221 54-55 22.87008465503747 26.46912325209688 26.95840118031253 23.702390912553113 56-57 23.14685534874097 26.415940216690508 27.048453988502747 23.388750446065774 58-59 23.723802295325115 26.21961075254049 26.930711121360268 23.125875830774127 60-61 23.15521948981382 26.554601436726028 27.564687907181078 22.72549116627907 62-63 22.74165947329366 26.584652468245324 27.534762471749424 23.138925586711593 64-65 22.53295436723147 27.0600509133241 27.138289140779932 23.268705578664502 66-67 22.9532578275352 26.461031196184877 26.70303760020812 23.882673376071804 68-69 22.55508400622077 26.45717640976491 27.18158874407206 23.806150839942255 70-71 23.329933237063077 26.259888604989428 26.792918543579287 23.61725961436821 72-73 23.054508487994458 26.04799217922998 27.307651939748883 23.58984739302668 74-75 22.400298676646546 27.219743246039386 26.728935949452875 23.651022127861193 76-77 22.778833722152648 26.29383034171327 26.7863914991517 24.14094443698238 78-79 22.802978538431354 26.38181592721261 26.973987944839372 23.841217589516667 80-81 23.152457492428105 26.255240351455907 26.678546795813716 23.91375536030228 82-83 22.968069187425595 26.052595278150125 27.234017350834105 23.74531818359018 84-85 22.670732391181595 26.382767661446714 26.962668684235226 23.983831263136462 86-87 22.47487337260346 26.42215135256819 26.96400405207669 24.13897122275166 88-89 22.51691203932388 26.374799706034725 26.556660602628824 24.551627652012577 90-91 22.76739172516316 25.814106533715027 26.71824955166151 24.700252189460308 92-93 22.919939065166087 26.209520468046698 26.666816604742245 24.203723862044978 94-95 22.750707602371627 25.89008259121987 26.848046579630736 24.51116322677777 96-97 22.119471294196885 25.847427766560738 27.386497592443597 24.646603346798777 98-99 22.440617025168606 26.17789852790797 26.50354903424886 24.877935412674564 100-101 22.572905424390882 25.003021264000896 27.271893068545662 25.15218024306256 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 723.0 1 519.5 2 375.5 3 467.5 4 436.5 5 453.0 6 741.0 7 1413.5 8 2278.5 9 2723.0 10 2577.0 11 2394.5 12 2563.5 13 2825.5 14 3074.0 15 3263.0 16 3517.0 17 3811.5 18 4141.5 19 4661.0 20 5169.0 21 5797.5 22 6901.0 23 8570.5 24 10282.0 25 12192.0 26 15318.5 27 19032.0 28 22344.5 29 26111.5 30 29796.0 31 34276.0 32 39680.0 33 45028.5 34 51192.0 35 56938.0 36 64085.5 37 71101.5 38 75998.5 39 80002.5 40 83047.5 41 85599.5 42 88813.5 43 94696.0 44 101613.5 45 107511.0 46 114176.5 47 134967.5 48 167167.0 49 166089.5 50 140128.0 51 129744.0 52 128570.5 53 128405.0 54 126701.5 55 120897.0 56 111596.5 57 101101.5 58 92978.0 59 85231.0 60 72972.0 61 59330.5 62 47196.5 63 35956.5 64 26656.5 65 19189.5 66 13139.5 67 9250.0 68 6528.5 69 4635.5 70 3305.5 71 1993.0 72 1207.0 73 748.5 74 448.5 75 283.5 76 192.0 77 91.5 78 46.0 79 21.5 80 14.5 81 13.5 82 10.0 83 7.0 84 4.5 85 3.5 86 2.5 87 4.5 88 4.5 89 1.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3922979809338743 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.546470307762894E-4 16-17 0.002114126865604505 18-19 4.198266116094053E-4 20-21 0.0 22-23 0.0 24-25 8.996284534487257E-5 26-27 2.9987615114957524E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012204959351787711 36-37 0.02289554414027007 38-39 0.010870510479172101 40-41 0.005802603524744281 42-43 0.005382776913134875 44-45 0.001499380755747876 46-47 0.0010945479516959497 48-49 0.002773854398133571 50-51 3.74845188936969E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.69764687184193E-4 60-61 0.0013494426801730884 62-63 0.0013044612575006521 64-65 6.89715147644023E-4 66-67 0.002623916322558783 68-69 0.002279058748736772 70-71 1.4993807557478762E-5 72-73 0.0 74-75 0.0 76-77 0.09123731898725827 78-79 0.003643495236467339 80-81 2.998761511495752E-4 82-83 0.0 84-85 8.996284534487257E-5 86-87 0.09251179262964396 88-89 0.43751930452723026 90-91 0.3898689841095627 92-93 0.0 94-95 0.001004585106351077 96-97 2.9987615114957524E-5 98-99 0.0 100-101 3.8983899649444777E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3334710.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.337335630746615 #Duplication Level Percentage of deduplicated Percentage of total 1 67.36136156632615 27.845392116113 2 15.190662600300037 12.558830367240652 3 6.200063536214444 7.688823219853505 4 3.242373600550341 5.361243430648872 5 1.9398387777731687 4.009388331317338 6 1.250595570197926 3.1017773294157966 7 0.8591147077581006 2.4859459113935176 8 0.635581320956591 2.101859070801271 9 0.49542399129060877 1.8431557026753587 >10 2.590279451491578 19.21833189463087 >50 0.1646893043389409 4.647320700739184 >100 0.06610434795309172 4.529883274123999 >500 0.0018831823351891242 0.5354589301409765 >1k 0.0013037416160462563 1.4553560538279353 >5k 5.794407182427805E-4 1.6402396868181326 >10k+ 1.4486017956069514E-4 0.9769939802596148 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22097 0.6626363311952164 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10534 0.31588953762096256 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9119 0.27345706223329763 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8462 0.25375519910277056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7589 0.22757601110741263 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7573 0.2270962092655733 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6129 0.18379409303957464 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5337 0.16004390186852832 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5320 0.159534112411574 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5254 0.1575549298139868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4931 0.14786893013185554 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4784 0.14346075070995679 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4653 0.13953237312989736 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4045 0.12129990314000319 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3575 0.10720572403597314 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1995046045983008E-4 2 0.0 0.0 0.0 0.0 1.1995046045983008E-4 3 0.0 0.0 0.0 0.0 1.1995046045983008E-4 4 0.0 0.0 0.0 0.0 1.1995046045983008E-4 5 0.0 0.0 0.0 0.0 1.1995046045983008E-4 6 0.0 0.0 0.0 0.0 1.4993807557478763E-4 7 0.0 0.0 0.0 0.0 1.4993807557478763E-4 8 0.0 0.0 0.0 0.0 1.4993807557478763E-4 9 0.0 0.0 0.0 0.0 1.7992569068974514E-4 10-11 0.0 0.0 0.0 1.499380755747876E-5 2.0991330580470265E-4 12-13 0.0 2.998761511495752E-5 0.0 2.998761511495752E-5 5.847584947416717E-4 14-15 0.0 2.998761511495752E-5 0.0 5.997523022991504E-5 7.796779929888956E-4 16-17 0.0 2.998761511495752E-5 0.0 1.4993807557478763E-4 9.296160685636832E-4 18-19 0.0 2.998761511495752E-5 0.0 1.949194982472239E-4 0.0011095417592534283 20-21 0.0 2.998761511495752E-5 0.0 2.0991330580470265E-4 0.0012144984121557796 22-23 0.0 2.998761511495752E-5 0.0 3.448575738220115E-4 0.0012744736423856947 24-25 0.0 2.998761511495752E-5 0.0 6.89715147644023E-4 0.001379430295288046 26-27 0.0 2.998761511495752E-5 0.0 0.0012144984121557796 0.0015593559859777913 28-29 0.0 2.998761511495752E-5 0.0 0.004018340425404308 0.0016493188313226638 30-31 0.0 2.998761511495752E-5 0.0 0.012309916004690061 0.0016943002539951 32-33 0.0 2.998761511495752E-5 0.0 0.02940285662021585 0.0018592321371273665 34-35 0.0 2.998761511495752E-5 0.0 0.05331797967439447 0.002069145442932069 36-37 0.0 2.998761511495752E-5 0.0 0.09306656350927067 0.0020991330580470264 38-39 0.0 2.998761511495752E-5 0.0 0.15350660177346756 0.0021741020958344205 40-41 0.0 2.998761511495752E-5 0.0 0.22106869862746686 0.0021890959033918993 42-43 0.0 2.998761511495752E-5 0.0 0.29230427833304845 0.002264064941179293 44-45 0.0 2.998761511495752E-5 0.0 0.3828668759802202 0.0024289968243115592 46-47 0.0 2.998761511495752E-5 0.0 0.49268152253119457 0.0025339534772139106 48-49 0.0 2.998761511495752E-5 0.0 0.6063945590471136 0.0025939287074438254 50-51 0.0 2.998761511495752E-5 0.0 0.7319976849561132 0.002638910130116262 52-53 0.0 2.998761511495752E-5 0.0 0.8579156808238197 0.0026688977452312196 54-55 0.0 2.998761511495752E-5 0.0 1.0002069145442931 0.002758860590576092 56-57 0.0 2.998761511495752E-5 0.0 1.180942270842142 0.002773854398133571 58-59 0.0 2.998761511495752E-5 0.0 1.3682299210426092 0.002803842013248528 60-61 0.0 2.998761511495752E-5 0.0 1.5783831277682316 0.00290879866615088 62-63 0.0 2.998761511495752E-5 0.0 1.7985671917498074 0.0030287491266107096 64-65 0.0 2.998761511495752E-5 0.0 2.0324256082238037 0.003133705779513061 66-67 0.0 2.998761511495752E-5 0.0 2.3017593733787947 0.0031486995870705398 68-69 0.0 2.998761511495752E-5 0.0 2.6147401123336063 0.0031486995870705398 70-71 0.0 2.998761511495752E-5 0.0 2.947422714418945 0.0032236686248579335 72-73 0.0 2.998761511495752E-5 0.0 3.2831490594384523 0.0033586128928752425 74-75 0.0 2.998761511495752E-5 0.0 3.6524315457715963 0.003433581930662636 76-77 0.0 2.998761511495752E-5 0.0 4.039166824101646 0.0035085509684500303 78-79 0.0 2.998761511495752E-5 0.0 4.49950670373136 0.003583520006237424 80-81 0.0 2.998761511495752E-5 0.0 4.999310284852356 0.0036135076213523816 82-83 0.0 2.998761511495752E-5 0.0 5.492156739266683 0.003928377580059436 84-85 0.0 2.998761511495752E-5 0.0 6.000296877389638 0.003928377580059436 86-87 0.0 2.998761511495752E-5 0.0 6.528438754794269 0.004153284693421617 88-89 0.0 2.998761511495752E-5 0.0 7.1220136083797385 0.004348204191668841 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10430 0.0 22.380844 1 TACATGG 10405 0.0 21.937363 2 GAGTACT 9360 0.0 21.219105 12-13 TTTGCGT 785 0.0 20.55373 3 AGAGTAC 14580 0.0 20.375944 10-11 ACATGGG 11070 0.0 19.847908 3 GTACTTT 10010 0.0 19.651894 14-15 GTAAGCG 675 0.0 18.983112 94-95 AGTACTT 9955 0.0 18.78289 12-13 CATGGGG 7720 0.0 16.842813 4 CGTACAC 285 8.6801083E-7 16.650856 3 GTATAGG 840 0.0 16.446875 1 ACTTTTT 11975 0.0 16.347425 16-17 GAGTACA 8740 0.0 16.079601 1 TACTTTT 11800 0.0 15.685434 14-15 AGTACAT 8190 0.0 15.528598 2 TATACAG 1850 0.0 14.877764 5 GTATAGA 810 0.0 14.7034645 1 CCCTATA 745 0.0 14.650517 2 CGATACG 100 0.0050615994 14.341634 86-87 >>END_MODULE