FastQCFastQC Report
Fri 27 May 2016
SRR939030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345306
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT214800.6420937277486723No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT111200.33240606389968513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91910.27474317745521637No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT90090.2693027184957071No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC76760.22945584051204884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72020.2152867331120083No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63410.18954917726509923No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51570.15415630139664355No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC49900.14916423191181913No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC48830.14596572032573402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45210.13514458767000687No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG44500.13302221082316534No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA43190.12910627607758451No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC41980.12548926764846025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103350.023.7643241
TACATGG103450.023.196862
GAGTACT90150.021.289912-13
ACATGGG108300.021.1091353
GTATAGG8250.020.6897281
TTTGCGT7050.020.141133
AGAGTAC139750.020.13470510-11
GTACTTT97100.019.57108114-15
AGTACTT93500.019.00845512-13
CATGGGG76450.018.449754
GTGTACG3357.512426E-1018.3994461
GAGTACA86100.017.0712261
AGTACAT79000.017.0154322
ACTTTTT117650.016.43530816-17
TACTTTT114200.015.56296414-15
TAACCCG2154.9218535E-415.4103085
CCTATAC8450.015.1237263
GTGTAGC17050.015.0167361
CTAGAGT10350.014.1766334
CCCTATA8050.014.1113022
Produced by FastQC (version 0.11.5)