##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939030_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3345306 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815766031567815 33.0 31.0 34.0 30.0 34.0 2 31.877000489641308 33.0 31.0 34.0 30.0 34.0 3 31.940911832878665 34.0 31.0 34.0 30.0 34.0 4 35.467688755527895 37.0 35.0 37.0 33.0 37.0 5 35.499391386019695 37.0 35.0 37.0 33.0 37.0 6 35.582762234605745 37.0 35.0 37.0 33.0 37.0 7 35.494385266997995 37.0 35.0 37.0 33.0 37.0 8 35.479465256691014 37.0 35.0 37.0 33.0 37.0 9 37.091323783235374 39.0 37.0 39.0 33.0 39.0 10-11 36.97397517596298 39.0 37.0 39.0 33.0 39.0 12-13 36.854643939896675 39.0 37.0 39.0 33.0 39.0 14-15 38.07063763374711 40.0 38.0 41.0 33.0 41.0 16-17 38.00205302594142 40.0 38.0 41.0 33.0 41.0 18-19 37.931726275563435 40.0 38.0 41.0 33.0 41.0 20-21 37.79757516950617 40.0 38.0 41.0 32.0 41.0 22-23 37.87712738386264 40.0 38.0 41.0 32.5 41.0 24-25 37.81336550378351 40.0 38.0 41.0 32.0 41.0 26-27 37.72539044260824 40.0 38.0 41.0 32.0 41.0 28-29 37.60966545362368 40.0 37.0 41.0 32.0 41.0 30-31 37.44520546102509 40.0 37.0 41.0 31.5 41.0 32-33 37.264786539706684 40.0 37.0 41.0 31.0 41.0 34-35 36.938451669294224 40.0 36.0 41.0 30.5 41.0 36-37 36.68055732420292 39.5 36.0 41.0 30.0 41.0 38-39 36.47840720699392 39.0 36.0 41.0 30.0 41.0 40-41 36.355469723845886 39.0 35.0 41.0 30.0 41.0 42-43 36.14623101743159 39.0 35.0 41.0 29.5 41.0 44-45 36.101887988722105 39.0 35.0 41.0 29.0 41.0 46-47 35.904201289807276 39.0 35.0 41.0 28.0 41.0 48-49 35.72422702138459 39.0 35.0 41.0 27.0 41.0 50-51 34.541948927840984 38.0 34.0 40.0 25.5 40.5 52-53 34.69853295931672 38.0 34.0 39.5 26.0 40.5 54-55 35.19830936243201 39.0 35.0 40.0 26.0 41.0 56-57 35.05923867652167 38.5 34.5 40.0 26.0 41.0 58-59 34.83661016361433 38.0 34.0 40.0 26.0 41.0 60-61 34.53366642692776 38.0 34.0 40.0 26.0 41.0 62-63 34.16937359392534 37.0 34.0 40.0 24.5 41.0 64-65 33.74483021882004 37.0 33.5 39.5 23.5 41.0 66-67 33.33136505300263 36.0 33.0 39.0 22.5 41.0 68-69 32.91181942698216 35.5 32.5 39.0 22.0 40.5 70-71 32.474697830333014 35.0 32.0 38.5 21.0 40.0 72-73 32.03449624638225 35.0 32.0 37.0 20.0 39.5 74-75 31.57886244188125 35.0 31.0 37.0 18.5 39.0 76-77 31.18159818563683 35.0 31.0 36.0 17.5 39.0 78-79 30.762908385660385 35.0 31.0 36.0 14.5 38.0 80-81 30.324192913891885 34.0 30.0 35.0 9.5 37.0 82-83 29.959786040499733 34.0 30.0 35.0 7.0 37.0 84-85 29.618283798253433 34.0 30.0 35.0 2.0 36.0 86-87 29.26458581068518 34.0 29.5 35.0 2.0 36.0 88-89 28.850282306013263 34.0 29.0 35.0 2.0 35.5 90-91 28.416257735465756 34.0 29.0 35.0 2.0 35.0 92-93 27.989626808429485 34.0 28.0 35.0 2.0 35.0 94-95 27.500898871433584 34.0 27.0 35.0 2.0 35.0 96-97 26.90335189067906 33.0 25.5 35.0 2.0 35.0 98-99 26.18014211554937 33.0 24.5 35.0 2.0 35.0 100-101 23.51824556557756 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37418017081973076 1101 2 0.4292241155530938 1101 3 0.39463519709638106 1101 4 0.3421070947813547 1101 5 0.19402440661248477 1101 6 0.29202554692322025 1101 7 0.30984267539668764 1101 8 0.37221417878858176 1101 9 0.36482819851578085 1101 10-11 0.4242693107141946 1101 12-13 0.5129974939086139 1101 14-15 0.33529242476315346 1101 16-17 0.5971134140176986 1101 18-19 0.5989489870985949 1101 20-21 0.6771091779057983 1101 22-23 0.6495133431332363 1101 24-25 0.687931370870416 1101 26-27 0.611491475295658 1101 28-29 0.5067582176067376 1101 30-31 0.6051785414157322 1101 32-33 0.4721618007161368 1101 34-35 0.36302587347951487 1101 36-37 0.44647605819228176 1101 38-39 0.4658158269623698 1101 40-41 0.46215661170010947 1101 42-43 0.33297817762272786 1101 44-45 0.3744123858201789 1101 46-47 0.40970759267310797 1101 48-49 0.5533845591755551 1101 50-51 0.4612329471270229 1101 52-53 0.2480125932634465 1101 54-55 0.3054173371873432 1101 56-57 0.42016763295595894 1101 58-59 0.34968006621019754 1101 60-61 0.392352443138293 1101 62-63 0.21644278099824987 1101 64-65 0.22921784372810805 1101 66-67 0.2357970760032444 1101 68-69 0.12692843938503984 1101 70-71 0.039199196851804174 1101 72-73 -0.10438769140736781 1101 74-75 -0.0999847983248685 1101 76-77 -0.1284263299199786 1101 78-79 -0.15188137342967778 1101 80-81 -0.2801518410147139 1101 82-83 -0.43310213904766215 1101 84-85 -0.579484174159056 1101 86-87 -0.5107792086817398 1101 88-89 -0.34052190394872994 1101 90-91 -0.4850886078824992 1101 92-93 -0.3696721928546758 1101 94-95 -0.3666358199799902 1101 96-97 -0.45779504222563006 1101 98-99 -0.4946943343306067 1101 100-101 -0.39759371772612084 1102 1 0.2305908903374565 1102 2 0.10624486184152104 1102 3 0.11001670966095034 1102 4 0.2200758529339737 1102 5 0.19191225062800754 1102 6 0.23173749098972252 1102 7 0.26716749187491473 1102 8 0.14616532527286807 1102 9 0.2805739451052247 1102 10-11 0.16496478698780948 1102 12-13 0.27025488193925895 1102 14-15 0.3597470771253555 1102 16-17 0.39130172401196006 1102 18-19 0.40291622811114536 1102 20-21 0.3943840050200649 1102 22-23 0.3850679019786014 1102 24-25 0.3613368686970517 1102 26-27 0.4159388429773614 1102 28-29 0.3761205170773465 1102 30-31 0.3281646182440312 1102 32-33 0.3460940832894934 1102 34-35 0.30114552285158425 1102 36-37 0.343573925767636 1102 38-39 0.34679468332774377 1102 40-41 0.23395897811753485 1102 42-43 0.24639633835127484 1102 44-45 0.26822261487622256 1102 46-47 0.41286619401445535 1102 48-49 0.36119000402269563 1102 50-51 0.22705635683912107 1102 52-53 0.20329729784256045 1102 54-55 0.32149490799724134 1102 56-57 0.2269817190797454 1102 58-59 0.1344368220991612 1102 60-61 0.03631609198056651 1102 62-63 0.07007950220971537 1102 64-65 0.09627483280710436 1102 66-67 0.15762914304639253 1102 68-69 0.09898376282083632 1102 70-71 0.012278088135481369 1102 72-73 0.05363398603580549 1102 74-75 0.07725077580026962 1102 76-77 0.027719090765305765 1102 78-79 -0.031391654261135216 1102 80-81 -0.14668084126756753 1102 82-83 -0.16845320847086498 1102 84-85 -0.1876198377874303 1102 86-87 -0.2678797653677911 1102 88-89 -0.3149942161424413 1102 90-91 -0.376518773282875 1102 92-93 -0.2715141333490685 1102 94-95 -0.34272129757808756 1102 96-97 -0.4447162312139241 1102 98-99 -0.529736256362451 1102 100-101 -0.1699266818084304 1103 1 -0.03143127311355798 1103 2 0.23542565430618012 1103 3 0.21550876075640346 1103 4 0.1983298155531159 1103 5 0.08486236807181768 1103 6 0.16199036496097818 1103 7 0.21229342595255218 1103 8 0.10767067680555442 1103 9 0.10668492802410157 1103 10-11 0.19070923872935452 1103 12-13 0.32518693019476785 1103 14-15 0.2875987133512936 1103 16-17 0.2988533312467325 1103 18-19 0.25010970569032054 1103 20-21 0.2410543103739613 1103 22-23 0.29488392640218564 1103 24-25 0.32610076818644984 1103 26-27 0.2348356107581182 1103 28-29 0.09180577359000353 1103 30-31 0.2675591761879019 1103 32-33 0.200502811405606 1103 34-35 0.14108514430978403 1103 36-37 0.26330167504729474 1103 38-39 0.21596035896983778 1103 40-41 0.2758179331338937 1103 42-43 0.048752758119704254 1103 44-45 0.08930126376952785 1103 46-47 -0.02772720783269733 1103 48-49 0.08240532555839764 1103 50-51 -0.10506141829592508 1103 52-53 -0.0051518522429390146 1103 54-55 0.0898186564959147 1103 56-57 0.08360501732784797 1103 58-59 0.05776258222979891 1103 60-61 0.042636314206738746 1103 62-63 0.008527409816643683 1103 64-65 -0.1406829880038174 1103 66-67 -0.12098351130198637 1103 68-69 -0.25194543365051913 1103 70-71 -0.24920729499166328 1103 72-73 -0.24233136064529504 1103 74-75 -0.3750778361145102 1103 76-77 -0.4716487243065508 1103 78-79 -0.41870106692377007 1103 80-81 -0.4024364753162146 1103 82-83 -0.3701336607330674 1103 84-85 -0.43217704209232366 1103 86-87 -0.3284158357039111 1103 88-89 -0.20677551624834933 1103 90-91 -0.2744299301487523 1103 92-93 -0.2634964487374667 1103 94-95 -0.21066727342667235 1103 96-97 -0.19467259654994962 1103 98-99 -0.21278322796885618 1103 100-101 0.05377078649357614 1104 1 0.12330708530600987 1104 2 0.13184038070944482 1104 3 0.11210987003831363 1104 4 0.1709799409842887 1104 5 0.15279275377266544 1104 6 0.19595722369412272 1104 7 0.13168596671768285 1104 8 0.12654071835462588 1104 9 0.11937111491654662 1104 10-11 0.09446065962931982 1104 12-13 0.2207432860273144 1104 14-15 0.15894420762850103 1104 16-17 0.08583788753396959 1104 18-19 0.19749956144447367 1104 20-21 0.14645456319616557 1104 22-23 0.14670213939999144 1104 24-25 0.18113344888573124 1104 26-27 0.19205814329183113 1104 28-29 0.15568321361822512 1104 30-31 0.13092798302390207 1104 32-33 0.16832285687440418 1104 34-35 0.056643753983657064 1104 36-37 0.0940161427487638 1104 38-39 0.16361366917679732 1104 40-41 0.2237250944697351 1104 42-43 0.13439260407454867 1104 44-45 0.2930801227378623 1104 46-47 0.3559027506182275 1104 48-49 0.32483976817364635 1104 50-51 0.0857031728768547 1104 52-53 0.2749158119935018 1104 54-55 0.13377576491548382 1104 56-57 0.19446206499168994 1104 58-59 0.2725519950551245 1104 60-61 0.30354191745226444 1104 62-63 0.18645823648644466 1104 64-65 0.1812689365806861 1104 66-67 0.16263209115959398 1104 68-69 0.18156139646863778 1104 70-71 0.10330108341849353 1104 72-73 -0.005694826857272517 1104 74-75 -0.04236793489155488 1104 76-77 -0.01365866080702105 1104 78-79 -0.13677687438692132 1104 80-81 -0.20060772806002092 1104 82-83 -0.2096747100431955 1104 84-85 -0.18751075759875135 1104 86-87 -0.18963291002187788 1104 88-89 -0.052765442557536346 1104 90-91 0.00567658835234397 1104 92-93 0.05026947514149427 1104 94-95 0.02069556562945607 1104 96-97 -0.15132098750322953 1104 98-99 -0.1440326400102485 1104 100-101 0.3016505587576077 1105 1 0.11821051546670347 1105 2 0.16333257786643074 1105 3 0.1960348545871362 1105 4 0.1590439088458453 1105 5 0.1546098118690793 1105 6 0.21282797462119163 1105 7 0.163176618757241 1105 8 0.20153106137069443 1105 9 0.32538934730220603 1105 10-11 0.22160354436356045 1105 12-13 0.3749446534562395 1105 14-15 0.30307411337633283 1105 16-17 0.3774710766563416 1105 18-19 0.40151532164225046 1105 20-21 0.3464087890923295 1105 22-23 0.22865342957304335 1105 24-25 0.3702606506780626 1105 26-27 0.263104767210244 1105 28-29 0.31397025780858456 1105 30-31 0.31202115360486715 1105 32-33 0.14182023017477263 1105 34-35 0.21689464242618328 1105 36-37 0.2748045389168752 1105 38-39 0.19657778382450886 1105 40-41 0.1952396958294358 1105 42-43 0.1749953930115069 1105 44-45 0.07824216692822006 1105 46-47 0.3317498612486318 1105 48-49 0.35578267608464387 1105 50-51 0.27825396861234 1105 52-53 0.30626450636927416 1105 54-55 0.28596847968680805 1105 56-57 0.2340966447197559 1105 58-59 0.09099181273127499 1105 60-61 0.39362245669825313 1105 62-63 0.2493914487855804 1105 64-65 0.3094538098810631 1105 66-67 0.24339132323623147 1105 68-69 0.18467461494823567 1105 70-71 0.10120455220712188 1105 72-73 0.22817472660378968 1105 74-75 0.22073543346443714 1105 76-77 0.1751712990199259 1105 78-79 0.08518696986524077 1105 80-81 0.024522834362720403 1105 82-83 0.039196774814655555 1105 84-85 0.15795412960767408 1105 86-87 0.10887914189406445 1105 88-89 0.1692671594202153 1105 90-91 0.22059817364282708 1105 92-93 0.28194109905991027 1105 94-95 0.24439708757012468 1105 96-97 -0.01504336852918442 1105 98-99 0.06630960350025816 1105 100-101 0.4088842191407984 1106 1 0.2435835496135148 1106 2 0.2464333108497705 1106 3 0.20447087522439844 1106 4 0.18404049620088614 1106 5 0.17101728092336543 1106 6 0.211348659020544 1106 7 0.24668234408193257 1106 8 0.2887175411124048 1106 9 0.3194817006395425 1106 10-11 0.33053686844567665 1106 12-13 0.3166576202249942 1106 14-15 0.3591555598249627 1106 16-17 0.42315866239669475 1106 18-19 0.35351618743593605 1106 20-21 0.43391004290940316 1106 22-23 0.37659469965014125 1106 24-25 0.23325888741531742 1106 26-27 0.2708048066536364 1106 28-29 0.2820213961990632 1106 30-31 0.2896745510531886 1106 32-33 0.3648394065998488 1106 34-35 0.2956581110611509 1106 36-37 0.13801686727591544 1106 38-39 0.22303681209930915 1106 40-41 0.29252600203532353 1106 42-43 0.3296121548677178 1106 44-45 0.2988863498580514 1106 46-47 0.34201909442543155 1106 48-49 0.3587886318100004 1106 50-51 0.3455530241581286 1106 52-53 0.1623358280093683 1106 54-55 0.07471755487888032 1106 56-57 0.3396393834785769 1106 58-59 0.5190755993870368 1106 60-61 0.2929667763601387 1106 62-63 0.29844086690133764 1106 64-65 0.1002734744086311 1106 66-67 0.25373842505221234 1106 68-69 0.37362296220993585 1106 70-71 0.4115583096235014 1106 72-73 0.24808920685230618 1106 74-75 0.23403192782961568 1106 76-77 0.1902867708586271 1106 78-79 0.2676656210371142 1106 80-81 0.22764082288567522 1106 82-83 0.4395537638738283 1106 84-85 0.40637356592169027 1106 86-87 0.4323828007773898 1106 88-89 0.5612251767225125 1106 90-91 0.5411820413724797 1106 92-93 0.5141682892049495 1106 94-95 0.4559292256355576 1106 96-97 0.5480346599952455 1106 98-99 0.5402859231380113 1106 100-101 0.8947258905147741 1107 1 0.044089520814356575 1107 2 -0.032007779381604706 1107 3 0.02428504075151139 1107 4 0.11078456920887447 1107 5 -0.008861267471644396 1107 6 -0.026185701192069644 1107 7 -0.023988824632844796 1107 8 0.019733529432016894 1107 9 0.0954048781942376 1107 10-11 0.06605706516801746 1107 12-13 0.11308087138391443 1107 14-15 0.0342090501929917 1107 16-17 0.04660230331545989 1107 18-19 0.11045289983597684 1107 20-21 0.023017774501170152 1107 22-23 -0.026822851115944957 1107 24-25 0.09385039691304087 1107 26-27 0.12211338798075388 1107 28-29 0.10668307135722443 1107 30-31 0.14473393901783282 1107 32-33 0.30209087660126244 1107 34-35 0.047402716426752534 1107 36-37 0.0671738627791143 1107 38-39 0.18585973328909233 1107 40-41 0.06387446852132683 1107 42-43 0.14734665377105927 1107 44-45 0.36405057986988254 1107 46-47 0.37568793650593335 1107 48-49 0.15759536377303363 1107 50-51 0.053756331071092234 1107 52-53 0.0476540991710408 1107 54-55 0.1796034868426446 1107 56-57 0.24917849139533388 1107 58-59 0.2809676817015685 1107 60-61 0.12196258323375275 1107 62-63 0.044508549460651636 1107 64-65 0.1551948660192366 1107 66-67 0.036249551326513085 1107 68-69 0.10127616316059118 1107 70-71 0.14340801627130162 1107 72-73 0.0012363662383236829 1107 74-75 0.07774871912968706 1107 76-77 0.022301600953220202 1107 78-79 0.1529139450364454 1107 80-81 0.1606014904528763 1107 82-83 0.22017330778685107 1107 84-85 0.08711675567894162 1107 86-87 0.09960646782334237 1107 88-89 0.15253520235900453 1107 90-91 0.15243256331440946 1107 92-93 0.06985715533876302 1107 94-95 0.09987969878575598 1107 96-97 0.0633179066545857 1107 98-99 0.23633249656031197 1107 100-101 0.32658962362862454 1108 1 0.16580281777031658 1108 2 0.08383700547667061 1108 3 0.12499685793402548 1108 4 0.13701423645294142 1108 5 0.2227058512276585 1108 6 0.16698162623912793 1108 7 0.17386027615281563 1108 8 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-1.4206740787412038 2316 80-81 -1.407738789415788 2316 82-83 -1.5003852957680834 2316 84-85 -1.7228389521806413 2316 86-87 -1.5588851366423668 2316 88-89 -1.7901074143536775 2316 90-91 -1.9789472055615605 2316 92-93 -2.180471262735793 2316 94-95 -2.3003279839746966 2316 96-97 -2.6251288950984772 2316 98-99 -3.0492704554481485 2316 100-101 -3.8323875875808397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10618.0 3 2720.0 4 2590.0 5 2996.0 6 3513.0 7 4259.0 8 5337.0 9 6618.0 10 8003.0 11 10226.0 12 13307.0 13 19215.0 14 18145.0 15 16132.0 16 20109.0 17 26039.0 18 23206.0 19 18053.0 20 18870.0 21 20481.0 22 22718.0 23 25571.0 24 29317.0 25 33807.0 26 39667.0 27 46175.0 28 55347.0 29 66095.0 30 79251.0 31 97092.0 32 120984.0 33 156162.0 34 210513.0 35 303188.0 36 469444.0 37 696321.0 38 562242.0 39 80959.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.034099595953904 18.54349146430886 13.898130499874393 23.524278439862844 2 15.462079529917213 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23.54777337469564 25.840664350385463 27.73433046536186 22.87723180955704 22-23 23.067626055160748 25.674156664473998 27.9914102747503 23.266807005614954 24-25 23.40321219268367 25.881661887686995 27.840883921676614 22.874241997952723 26-27 23.00105060566968 25.62708587129931 28.632745971929975 22.73911755110104 28-29 22.540925035776812 26.767175450859654 28.0579091257768 22.633990387586735 30-31 23.04194383696214 25.867229100094047 27.647303963195423 23.443523099748386 32-33 22.934413807921793 26.04292291937618 27.96037727831248 23.062285994389544 34-35 23.618911272802347 25.949377534093994 27.73018995652581 22.701521236577854 36-37 23.33315493277055 25.680390180071317 28.226792617463992 22.759662269694143 38-39 22.921257451727236 26.32611433948311 28.030925231706256 22.721702977083403 40-41 23.101783783743173 26.38969172388353 27.62518528646997 22.883339205903322 42-43 22.752088952281184 26.353601249275755 27.275678576018024 23.61863122242504 44-45 23.100302809045097 25.85235792831891 27.5345919834902 23.512747279145803 46-47 23.66868637068057 26.396384073448885 27.26147977014155 22.673449785728994 48-49 23.383118535950903 26.26901750287575 27.269798251681966 23.078065709491383 50-51 22.508816641959474 26.6519581070637 27.845239614673023 22.993985636303805 52-53 23.186379289245718 27.00203887911861 26.664245228358368 23.1473366032773 54-55 22.8468778866874 26.38461405482761 27.08050818475701 23.68799987372798 56-57 23.157258652914557 26.33033587944341 27.073505271780856 23.438900195861173 58-59 23.747868551763293 26.16021638247607 26.936501589222235 23.155413476538403 60-61 23.15984649929783 26.4353592321961 27.675530756464077 22.729263512041996 62-63 22.729194639901145 26.477494075173112 27.63090947652142 23.16240180840432 64-65 22.581540035442448 26.956604501545833 27.233759879665275 23.228095583346438 66-67 22.943066360799016 26.355340315356468 26.81504405682161 23.88654926702291 68-69 22.581679870757657 26.350962497855207 27.359063002033285 23.708294629353848 70-71 23.352900637124083 26.10581682302916 26.973390682370436 23.567891857476326 72-73 22.980024776752856 25.93995809995175 27.555078204351524 23.52493891894387 74-75 22.373715199884526 27.170447642703778 26.992455088379813 23.463382069031884 76-77 22.806430978379748 26.19166203763907 26.960017979919915 24.041889004061268 78-79 22.822073994805695 26.28303554251635 27.176768709118893 23.71812175355906 80-81 23.09744223580473 26.204034104324148 26.90675835937226 23.791765300498856 82-83 22.942242271853274 25.93435509491909 27.542108433952254 23.581294199275376 84-85 22.700557018898117 26.284109402636542 27.24278501632092 23.772548562144422 86-87 22.469119546514996 26.3376970382392 27.310079781049666 23.88310363419614 88-89 22.570897903561264 26.249689003073513 26.87900396798254 24.300409125382686 90-91 22.734013274565804 25.74094524711456 27.137503542665726 24.387537935653913 92-93 23.011779402683132 26.02006132765517 27.13366063670033 23.834498632961367 94-95 22.825799468947856 25.754390111766345 27.31037680824699 24.10943361103881 96-97 22.186410328620568 25.66125493756316 27.870342233965502 24.28199249985077 98-99 22.54272689804893 26.020317408191467 27.037300507190903 24.399655186568697 100-101 22.576356390455242 25.1227670350254 27.611195379455594 24.689681195063763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3429.0 1 1987.5 2 594.5 3 653.5 4 599.5 5 614.5 6 926.0 7 1708.0 8 2725.5 9 3309.5 10 3190.0 11 3045.5 12 3302.0 13 3630.5 14 4020.5 15 4406.0 16 4838.0 17 5161.0 18 5318.0 19 5589.0 20 5924.0 21 6351.0 22 7349.0 23 8784.0 24 10274.5 25 12153.5 26 15124.5 27 18807.0 28 22187.5 29 25800.5 30 29455.0 31 34110.0 32 39586.5 33 45142.0 34 51263.5 35 57133.5 36 64148.5 37 71028.0 38 75863.5 39 80278.5 40 83530.5 41 85758.5 42 89352.0 43 95525.0 44 101944.5 45 107558.5 46 115321.5 47 135200.0 48 167705.5 49 168199.5 50 140855.5 51 128069.5 52 125482.5 53 125438.0 54 124135.0 55 118878.5 56 109892.5 57 99748.5 58 91885.5 59 84402.0 60 72518.5 61 59194.0 62 47362.0 63 36543.5 64 27457.5 65 19897.0 66 13755.0 67 9590.5 68 6870.0 69 5025.5 70 3630.5 71 2209.0 72 1373.5 73 944.5 74 616.5 75 387.0 76 259.5 77 159.5 78 116.0 79 84.5 80 60.5 81 46.0 82 44.0 83 35.0 84 27.0 85 22.0 86 14.5 87 16.0 88 14.5 89 12.0 90 10.0 91 3.5 92 4.0 93 5.5 94 4.5 95 4.5 96 3.5 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28541484695271524 2 0.08716691387873038 3 0.09102306336101988 4 0.1068661581332171 5 0.10895864234841297 6 0.08352001281796045 7 0.09386286336735712 8 0.0904849959913981 9 0.09511835389647463 10-11 0.08899036440911534 12-13 0.09299597704963312 14-15 0.1017096791743416 16-17 0.08993198230595348 18-19 0.10103709496231436 20-21 0.1032790423357385 22-23 0.11574426973197668 24-25 0.12811981923327792 26-27 0.13081015608138688 28-29 0.12272419922123715 30-31 0.11578910867944517 32-33 0.11024402550917614 34-35 0.3812506240086856 36-37 0.581620933929512 38-39 0.5648511675762994 40-41 0.5287558148641709 42-43 0.555360257028804 44-45 0.5764046697073452 46-47 0.5652995570509842 48-49 0.5874649434162376 50-51 0.5784672612908954 52-53 0.5816807191928033 54-55 0.5725485202250556 56-57 0.544180412793329 58-59 0.5395470548882524 60-61 0.488176567405194 62-63 0.5631024486250286 64-65 0.5953117592232221 66-67 0.607971288725157 68-69 0.6094958129390854 70-71 0.6064168718795829 72-73 0.5981963981770277 74-75 0.594444872905498 76-77 0.5791248991870998 78-79 0.5555844517661463 80-81 0.5499197980692947 82-83 0.5647913823130082 84-85 0.5741477760180982 86-87 0.5646568654706027 88-89 0.5771818781301322 90-91 0.5910819518453618 92-93 0.5994219960744996 94-95 0.5984654318618386 96-97 0.5950277792225883 98-99 0.5987344655466496 100-101 0.5766587570763333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3345306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.07295071728326 #Duplication Level Percentage of deduplicated Percentage of total 1 69.34978845368074 29.871000203194104 2 14.277702500506553 12.299655523207017 3 5.877783866082193 7.595204843718029 4 3.041989551126176 5.241098640725936 5 1.7857496128754666 3.845875253439633 6 1.1615888060288317 3.001983443749667 7 0.8358082775359137 2.5200510123181274 8 0.5852527431261327 2.0166850049461407 9 0.4338793779950414 1.6819618559063343 >10 2.4349778768331243 18.706933177416353 >50 0.15162266115142553 4.464521684333391 >100 0.06058978372142838 4.337342612172317 >500 0.00125099591659109 0.36413667528358673 >1k 0.0014594952353872205 1.7432399707730128 >5k 4.1699863868206293E-4 1.334409440303227 >10k+ 1.389995462273543E-4 0.975900658513218 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21480 0.6420937277486723 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11120 0.33240606389968513 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9191 0.27474317745521637 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9009 0.2693027184957071 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7676 0.22945584051204884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7202 0.2152867331120083 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6341 0.18954917726509923 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5157 0.15415630139664355 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4990 0.14916423191181913 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4883 0.14596572032573402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4521 0.13514458767000687 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4450 0.13302221082316534 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4319 0.12910627607758451 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4198 0.12548926764846025 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0924842151958596E-4 2 0.0 0.0 0.0 0.0 2.391410531652411E-4 3 0.0 0.0 0.0 0.0 2.391410531652411E-4 4 0.0 0.0 0.0 0.0 2.391410531652411E-4 5 0.0 0.0 0.0 0.0 2.391410531652411E-4 6 0.0 0.0 0.0 0.0 2.391410531652411E-4 7 0.0 0.0 0.0 0.0 2.391410531652411E-4 8 0.0 0.0 0.0 0.0 3.288189481022065E-4 9 0.0 0.0 0.0 0.0 3.8860421139351675E-4 10-11 0.0 0.0 0.0 0.0 3.8860421139351675E-4 12-13 0.0 0.0 0.0 0.0 7.473157911413785E-4 14-15 0.0 0.0 0.0 0.0 9.416178968381368E-4 16-17 0.0 0.0 0.0 4.4838947468482706E-5 0.0011359200025348952 18-19 0.0 0.0 0.0 1.3451684240544813E-4 0.0012255978974718606 20-21 0.0 0.0 0.0 2.391410531652411E-4 0.0014348463189914466 22-23 0.0 0.0 0.0 3.8860421139351675E-4 0.0015095778981055845 24-25 0.0 0.0 0.0 5.978526329131026E-4 0.0015843094772197222 26-27 0.0 0.0 0.0 0.0012554905291175156 0.0016142021088653773 28-29 0.0 0.0 0.0 0.004558626325962409 0.0016440947405110325 30-31 0.0 0.0 0.0 0.013556308451304604 0.00165904105633386 32-33 0.0 0.0 0.0 0.03204490112414231 0.0017188263196251702 34-35 0.0 0.0 0.0 0.05830557802485034 0.0018533431620306184 36-37 0.0 0.0 0.0 0.10067838338256649 0.0018533431620306184 38-39 0.0 0.0 0.0 0.16261591615236393 0.0020028063202588943 40-41 0.0 0.0 0.0 0.23402941315383408 0.0020625915835502046 42-43 0.0 0.0 0.0 0.3105993891141797 0.002122376846841515 44-45 0.0 0.0 0.0 0.40361031247963564 0.002286786320892618 46-47 0.0 0.0 0.0 0.5156478958875511 0.0025408736898806863 48-49 0.0 0.0 0.0 0.6301217287745875 0.002660444216463307 50-51 0.0 0.0 0.0 0.7615596301205331 0.0027501221114002725 52-53 0.0 0.0 0.0 0.8943128072588875 0.003004209480388341 54-55 0.0 0.0 0.0 1.0428941328536163 0.0030490484278568237 56-57 0.0 0.0 0.0 1.2274811332655369 0.003138726322793789 58-59 0.0 0.0 0.0 1.424757555811038 0.0032732431651992374 60-61 0.0 0.0 0.0 1.6374735226015198 0.003392813691781858 62-63 0.0 0.0 0.0 1.8616682599439334 0.0036469010607699266 64-65 0.0 0.0 0.0 2.0964449888889085 0.0037216326398840645 66-67 0.0 0.0 0.0 2.372159676872609 0.003781417903175375 68-69 0.0 0.0 0.0 2.6893354449488327 0.0038262568506438575 70-71 0.0 0.0 0.0 3.025149268856123 0.0039009884297579954 72-73 0.0 0.0 0.0 3.369572170677361 0.004035505272163444 74-75 0.0 0.0 0.0 3.732932054646122 0.0041849684303917195 76-77 0.0 0.0 0.0 4.11192578496556 0.004259700009505856 78-79 0.0 0.0 0.0 4.571121445990292 0.004409163167734133 80-81 0.0 0.0 0.0 5.07485712816705 0.004439055799379787 82-83 0.0 0.0 0.0 5.57437794928177 0.004648304220899374 84-85 0.0 0.0 0.0 6.081177626202207 0.004693143168367856 86-87 0.0 0.0 0.0 6.615627987394875 0.004827660010773305 88-89 0.0 0.0 0.0 7.203347018180101 0.004992069484824408 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10335 0.0 23.764324 1 TACATGG 10345 0.0 23.19686 2 GAGTACT 9015 0.0 21.2899 12-13 ACATGGG 10830 0.0 21.109135 3 GTATAGG 825 0.0 20.689728 1 TTTGCGT 705 0.0 20.14113 3 AGAGTAC 13975 0.0 20.134705 10-11 GTACTTT 9710 0.0 19.571081 14-15 AGTACTT 9350 0.0 19.008455 12-13 CATGGGG 7645 0.0 18.44975 4 GTGTACG 335 7.512426E-10 18.399446 1 GAGTACA 8610 0.0 17.071226 1 AGTACAT 7900 0.0 17.015432 2 ACTTTTT 11765 0.0 16.435308 16-17 TACTTTT 11420 0.0 15.562964 14-15 TAACCCG 215 4.9218535E-4 15.410308 5 CCTATAC 845 0.0 15.123726 3 GTGTAGC 1705 0.0 15.016736 1 CTAGAGT 1035 0.0 14.176633 4 CCCTATA 805 0.0 14.111302 2 >>END_MODULE