FastQCFastQC Report
Fri 27 May 2016
SRR939030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345306
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT223770.6689074183348249No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT105650.3158156533363465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93790.28036299220459954No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT84270.25190520687793583No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC77470.23157821735889034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76310.22811067208799435No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT61150.18279344251318114No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC53240.15914837088146794No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC52230.1561292150852568No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51320.15340898560550215No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG48810.14590593506244273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48760.14575647190421442No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA47340.1415117182105314No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC41500.12405442132946883No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35300.10552098970916263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6750.028.823253
GTACATG100250.022.570961
TACATGG99550.022.1176822
GAGTACT94750.020.96066512-13
AGAGTAC144450.019.85946710-11
ACATGGG105700.019.6186583
GTACTTT101300.019.30085814-15
GAGTACA85950.018.8440321
AGTACTT102200.018.4808112-13
AGTACAT80400.018.3555432
CTTTGCG11700.017.4399592
GTGTAGC19050.017.0041431
ACTTTTT118650.016.41779116-17
CATGGGG72850.016.4147344
TACTTTT116550.015.859314-15
GTATTAG8250.015.0127491
TTGCGTT12700.014.9457984
TCTAGAC14800.014.74887853
TAGGACC10450.014.5310354
CGTACAC3606.175942E-714.49953
Produced by FastQC (version 0.11.5)