##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939030_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3345306 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13940458660583 33.0 31.0 34.0 30.0 34.0 2 32.36677153001848 34.0 31.0 34.0 30.0 34.0 3 32.47088666926135 34.0 31.0 34.0 30.0 34.0 4 36.043946054561225 37.0 35.0 37.0 35.0 37.0 5 35.95512846956302 37.0 35.0 37.0 35.0 37.0 6 35.99679670559285 37.0 35.0 37.0 35.0 37.0 7 35.937139980617616 37.0 35.0 37.0 35.0 37.0 8 35.902212533023885 37.0 35.0 37.0 35.0 37.0 9 37.57974666592533 39.0 37.0 39.0 35.0 39.0 10-11 37.494155392660645 39.0 37.0 39.0 34.5 39.0 12-13 37.427309639237784 39.0 37.0 39.0 34.0 39.0 14-15 38.67760124185949 40.0 38.0 41.0 34.0 41.0 16-17 38.67577838918174 40.0 38.0 41.0 34.0 41.0 18-19 38.764737814717094 40.0 38.0 41.0 34.0 41.0 20-21 38.75290167775384 40.0 38.0 41.0 34.0 41.0 22-23 38.691598317164406 40.0 38.0 41.0 34.0 41.0 24-25 38.62434213790905 40.0 38.0 41.0 34.0 41.0 26-27 38.46311040006505 40.0 38.0 41.0 34.0 41.0 28-29 38.2900864674263 40.0 38.0 41.0 34.0 41.0 30-31 38.142196408938375 40.0 38.0 41.0 33.0 41.0 32-33 38.00098570952852 40.0 37.0 41.0 33.0 41.0 34-35 37.978124721624866 40.0 37.0 41.0 33.0 41.0 36-37 37.881958033136584 40.0 37.0 41.0 33.0 41.0 38-39 37.758926836588344 40.0 37.0 41.0 33.0 41.0 40-41 37.62725771573662 40.0 37.0 41.0 33.0 41.0 42-43 37.46163026640911 40.0 37.0 41.0 32.0 41.0 44-45 37.22070238118725 40.0 36.5 41.0 31.0 41.0 46-47 36.93949208233866 40.0 36.0 41.0 31.0 41.0 48-49 36.65705439203469 39.0 36.0 41.0 30.5 41.0 50-51 36.38643684613605 39.0 35.0 41.0 30.0 41.0 52-53 36.16844677288117 39.0 35.0 40.5 29.5 41.0 54-55 35.912867313184506 39.0 35.0 40.0 29.0 41.0 56-57 35.59823002738763 38.0 35.0 40.0 28.0 41.0 58-59 35.27186376971195 38.0 34.0 40.0 28.0 41.0 60-61 34.82586406146403 37.5 34.0 40.0 27.0 41.0 62-63 34.50379337495583 37.0 34.0 40.0 26.0 41.0 64-65 34.233055511214815 36.5 33.5 39.0 26.0 41.0 66-67 33.75653437981458 36.0 33.0 39.0 26.0 40.0 68-69 33.314756706860294 35.5 33.0 38.5 25.5 40.0 70-71 32.84068094219184 35.0 32.0 37.5 25.0 40.0 72-73 32.314397845817396 35.0 32.0 37.0 23.5 39.0 74-75 31.862801190683307 35.0 31.0 36.5 22.5 39.0 76-77 29.90913402241828 33.0 28.5 35.0 20.0 36.5 78-79 30.905147541062014 34.0 30.5 35.0 20.0 37.0 80-81 30.91415673185054 34.0 31.0 35.0 20.0 37.0 82-83 30.60086222306719 34.0 31.0 35.0 19.5 36.5 84-85 30.215248620006662 34.0 30.0 35.0 18.0 36.0 86-87 29.681805341574133 34.0 30.0 35.0 11.0 36.0 88-89 29.121114779933436 34.0 29.0 35.0 6.0 35.0 90-91 28.661364012739043 33.5 29.0 35.0 2.0 35.0 92-93 28.29912868957279 33.0 29.0 35.0 2.0 35.0 94-95 27.895637648693423 33.0 27.5 35.0 2.0 35.0 96-97 27.255494863549103 33.0 27.0 35.0 2.0 35.0 98-99 26.35276533746091 33.0 25.0 34.0 2.0 35.0 100-101 21.997176192551592 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17237178668457886 1101 2 0.15721123844620877 1101 3 0.17941294739883062 1101 4 0.09454283708380729 1101 5 0.1497211224626369 1101 6 0.17398156633305462 1101 7 0.1966851454494858 1101 8 0.19954842807918993 1101 9 0.3404710100562127 1101 10-11 0.30874413493442177 1101 12-13 0.28721466790585737 1101 14-15 0.319261090331743 1101 16-17 0.304605638129928 1101 18-19 0.31343275243759905 1101 20-21 0.2789148830949628 1101 22-23 0.25130155469251747 1101 24-25 0.32492052988617104 1101 26-27 0.29206009407341327 1101 28-29 0.3409389049650571 1101 30-31 0.24249190955176658 1101 32-33 0.33379827588721867 1101 34-35 0.31221947902211866 1101 36-37 0.2971990581263455 1101 38-39 0.3657427593175697 1101 40-41 0.256452387279559 1101 42-43 0.3355888312524229 1101 44-45 0.28208231836626396 1101 46-47 0.20531315918890414 1101 48-49 -0.04624615990268666 1101 50-51 2.9165841115030844E-4 1101 52-53 0.07179740571896076 1101 54-55 0.14216360628458347 1101 56-57 -0.1770005318446053 1101 58-59 -0.06282971987803165 1101 60-61 0.12713701183886172 1101 62-63 -0.04854156068274307 1101 64-65 -0.029609396985414094 1101 66-67 0.07921753684325239 1101 68-69 0.01206218066992193 1101 70-71 0.05526791028915312 1101 72-73 -0.03504151125085997 1101 74-75 -0.10929679897003552 1101 76-77 -0.169709291248882 1101 78-79 0.06716327451867699 1101 80-81 0.3261576370816961 1101 82-83 0.23619339154370067 1101 84-85 0.23743124008547767 1101 86-87 0.29815221912221546 1101 88-89 0.3053746364312673 1101 90-91 0.2658838796170535 1101 92-93 0.3083910251006863 1101 94-95 0.40840628306952453 1101 96-97 0.3034987022045854 1101 98-99 0.29585241750133306 1101 100-101 0.9203031701147779 1102 1 0.12990029326487473 1102 2 0.06965198770470238 1102 3 0.09594997484782652 1102 4 0.09184610054040121 1102 5 0.11475846899267594 1102 6 0.13995026829530133 1102 7 0.10438884558554662 1102 8 0.14758156434486125 1102 9 0.21500212146165154 1102 10-11 0.2172180445401679 1102 12-13 0.23250170037184859 1102 14-15 0.2563867721417168 1102 16-17 0.2500086635801537 1102 18-19 0.19184812371549498 1102 20-21 0.21878857516369266 1102 22-23 0.2526343702717071 1102 24-25 0.1617697751290521 1102 26-27 0.2684365575316079 1102 28-29 0.24461255127877024 1102 30-31 0.16943599611705196 1102 32-33 0.1565051066297798 1102 34-35 0.19023640035157996 1102 36-37 0.17270416944349876 1102 38-39 0.2242349307945375 1102 40-41 0.11818212763989777 1102 42-43 0.18527878080148952 1102 44-45 0.17287803425168846 1102 46-47 0.24740295943212942 1102 48-49 0.20190758498499406 1102 50-51 0.2322928785449605 1102 52-53 0.2265423816975769 1102 54-55 0.2892985996420734 1102 56-57 0.11769820748423854 1102 58-59 0.10717746446251653 1102 60-61 0.09173075544219245 1102 62-63 0.20217877983091626 1102 64-65 0.050081295079301924 1102 66-67 0.12103029847968116 1102 68-69 0.10768053565661262 1102 70-71 0.2513202355671993 1102 72-73 0.24684070570016559 1102 74-75 0.30892172539049767 1102 76-77 0.11645653858258598 1102 78-79 0.06475802553512011 1102 80-81 0.3157921187054349 1102 82-83 0.3645906012903204 1102 84-85 0.2167936198280458 1102 86-87 0.31866862871792634 1102 88-89 0.41846037089964483 1102 90-91 0.44833693055362644 1102 92-93 0.3984655977528284 1102 94-95 0.39413713921013027 1102 96-97 0.256386385676187 1102 98-99 0.33988008323283125 1102 100-101 0.9238698540399142 1103 1 -0.00699658693061167 1103 2 -0.002468864043940755 1103 3 0.004636379174023375 1103 4 -0.022064926161057485 1103 5 0.021093038888011506 1103 6 -0.010348222389069406 1103 7 0.03839903390979771 1103 8 0.06368053663758388 1103 9 0.03523351830339294 1103 10-11 0.09428810258166465 1103 12-13 -0.03719395154481475 1103 14-15 0.06258925473222376 1103 16-17 -0.04285240956633629 1103 18-19 -0.02176603935685506 1103 20-21 0.06375890359219483 1103 22-23 0.05552742244988451 1103 24-25 0.029062710159259098 1103 26-27 -0.07516867152818918 1103 28-29 0.09661412508494749 1103 30-31 -0.01382722592570218 1103 32-33 0.05252518153348262 1103 34-35 -0.0036239805568527572 1103 36-37 0.024156833968362434 1103 38-39 0.03232612872137963 1103 40-41 0.12589170714965547 1103 42-43 0.09478670012322254 1103 44-45 0.13689008629378918 1103 46-47 0.1734373228393551 1103 48-49 0.09407375761851 1103 50-51 0.01286554283316832 1103 52-53 0.07203529069489178 1103 54-55 0.1055323216937083 1103 56-57 0.04568962254899844 1103 58-59 -0.04806254098073026 1103 60-61 -0.0925928628920687 1103 62-63 0.012023203764606194 1103 64-65 0.026032974496168038 1103 66-67 0.02271932976055524 1103 68-69 0.04688398856372089 1103 70-71 -0.1490550225429388 1103 72-73 -0.006092135374991869 1103 74-75 -0.0862255083197212 1103 76-77 -0.22513143777482014 1103 78-79 -0.009030557861819943 1103 80-81 0.05669924639081714 1103 82-83 0.0405628407691907 1103 84-85 -0.10056158704463769 1103 86-87 -0.181060836365468 1103 88-89 0.008141586398679124 1103 90-91 0.1932056419850241 1103 92-93 0.09903033384446402 1103 94-95 0.09959901582609376 1103 96-97 0.026463212267593406 1103 98-99 -0.1268680841112797 1103 100-101 0.5001722378237972 1104 1 -0.04313036711248941 1104 2 -0.044683704119201195 1104 3 -0.0032854811270155437 1104 4 -0.01215547178664167 1104 5 0.007085513017834444 1104 6 0.011546180244984328 1104 7 -0.025292758188037112 1104 8 -0.017807586598536318 1104 9 0.017132624606304603 1104 10-11 0.028156527244568963 1104 12-13 0.037512510120279785 1104 14-15 -0.02734259106583181 1104 16-17 0.019307995341158346 1104 18-19 -0.016079352699598815 1104 20-21 -0.006447273892909777 1104 22-23 -0.013632335703135823 1104 24-25 -0.06901344027975398 1104 26-27 -0.07094335599040136 1104 28-29 -0.009617991174060592 1104 30-31 0.040528763412652324 1104 32-33 0.03758706417694668 1104 34-35 0.05779143179812252 1104 36-37 0.0629871883114177 1104 38-39 0.15329939620333732 1104 40-41 0.0819743839292073 1104 42-43 0.036838332688283515 1104 44-45 0.10791930783658898 1104 46-47 0.07203739339438897 1104 48-49 0.01393392146297856 1104 50-51 0.021734702444334175 1104 52-53 -0.13744936358544635 1104 54-55 0.0032009188244686015 1104 56-57 -0.003472193459153061 1104 58-59 -0.04857626981421248 1104 60-61 0.06299254789501418 1104 62-63 0.05116757700073293 1104 64-65 -0.004911825989886154 1104 66-67 -0.004847256551769874 1104 68-69 0.09764221018491526 1104 70-71 -0.0057955270114220525 1104 72-73 -0.044359176375301956 1104 74-75 -0.06408141926359434 1104 76-77 -0.14673725072558952 1104 78-79 0.10582622522065321 1104 80-81 0.16688783411424168 1104 82-83 0.2331499566362325 1104 84-85 -0.007578432058750195 1104 86-87 -0.13533294360911796 1104 88-89 0.02201481272354755 1104 90-91 0.1091260421888478 1104 92-93 0.04933430844465647 1104 94-95 0.040798892354786176 1104 96-97 0.021855135676190685 1104 98-99 0.04664403763534963 1104 100-101 0.6561290914613025 1105 1 0.002287587152039805 1105 2 0.020873310713923843 1105 3 0.04145378030685265 1105 4 0.028541376173798483 1105 5 -0.009577033335688157 1105 6 0.03176095156760539 1105 7 -0.01605218340435499 1105 8 0.013005917727788585 1105 9 -0.022732950177378086 1105 10-11 0.014161432992402467 1105 12-13 0.08379862724017784 1105 14-15 0.11484935114678052 1105 16-17 0.016548222473417695 1105 18-19 0.0032726636786435392 1105 20-21 0.088438387417348 1105 22-23 0.019831624432832484 1105 24-25 0.03296334433088077 1105 26-27 0.0014266610058868423 1105 28-29 0.09770103694707188 1105 30-31 0.04066318862897589 1105 32-33 0.06490879625358303 1105 34-35 0.12821324885621266 1105 36-37 0.2515162365190875 1105 38-39 0.23196595986217972 1105 40-41 0.22617922787235045 1105 42-43 0.22998631631003974 1105 44-45 0.1245374320640309 1105 46-47 0.21088396323369807 1105 48-49 0.18999657597472464 1105 50-51 0.2538314267953865 1105 52-53 0.2773254355492938 1105 54-55 0.22049908786031125 1105 56-57 0.2698036486298392 1105 58-59 0.1975194115825687 1105 60-61 0.05010704109650277 1105 62-63 0.11059646297105985 1105 64-65 0.1408605395848994 1105 66-67 0.16912581977702956 1105 68-69 0.08955834893646397 1105 70-71 0.23402813646200116 1105 72-73 0.30934041416857383 1105 74-75 0.32047667715173134 1105 76-77 0.15708807374906897 1105 78-79 0.18687516681521288 1105 80-81 0.18523513788433021 1105 82-83 0.32521848259420594 1105 84-85 0.11105394587697504 1105 86-87 0.14303386726850675 1105 88-89 0.24350604418213706 1105 90-91 0.389180700717894 1105 92-93 0.5609141910157298 1105 94-95 0.32126609723735555 1105 96-97 0.3131230976662991 1105 98-99 0.4235651980185402 1105 100-101 1.0444022295947946 1106 1 -0.03816583080986646 1106 2 -0.07021489691968696 1106 3 -0.06592066264013141 1106 4 -0.036202909248508774 1106 5 -0.07243248576190808 1106 6 -0.05473432616135909 1106 7 0.0019460307076286654 1106 8 -0.04402974950274796 1106 9 -0.10876045043945481 1106 10-11 0.021966491399545873 1106 12-13 0.0012690880641414992 1106 14-15 0.0014214228145803531 1106 16-17 -0.051649810485564274 1106 18-19 -0.10250905449947822 1106 20-21 -0.07523159360005138 1106 22-23 0.08321339721327092 1106 24-25 0.08232169166046077 1106 26-27 0.03473398156298657 1106 28-29 0.07863179909438855 1106 30-31 -0.017832279906443205 1106 32-33 0.002650650967666479 1106 34-35 0.09528914381764508 1106 36-37 -0.10713483731395712 1106 38-39 0.056611629272289576 1106 40-41 0.05799682520437699 1106 42-43 0.15684796127278844 1106 44-45 0.11674538318741412 1106 46-47 0.06835107551763997 1106 48-49 -0.037057346078206876 1106 50-51 -0.09584995283327657 1106 52-53 0.13150255069155747 1106 54-55 0.08258364075857827 1106 56-57 0.2001411950399259 1106 58-59 0.16658303082641623 1106 60-61 0.2180379261744534 1106 62-63 0.12186893834910961 1106 64-65 0.03519656708515839 1106 66-67 0.15252509320418284 1106 68-69 0.1591598085680772 1106 70-71 0.1359525854046879 1106 72-73 0.29532632164178096 1106 74-75 0.3032202890951652 1106 76-77 0.14466623463133388 1106 78-79 0.256048254659337 1106 80-81 0.33852879277806025 1106 82-83 0.3683766573683869 1106 84-85 0.29456855162032625 1106 86-87 0.3513121143530533 1106 88-89 0.3955755776716856 1106 90-91 0.5608669093334484 1106 92-93 0.5518583322396502 1106 94-95 0.518818740585413 1106 96-97 0.6967616144742372 1106 98-99 0.7064630011801931 1106 100-101 1.157724290515599 1107 1 0.003416484177343193 1107 2 -0.10607691352739579 1107 3 -0.08415090221957655 1107 4 -0.05235084356205988 1107 5 -0.1538097828850482 1107 6 -0.07207863007367621 1107 7 -0.0924674546979034 1107 8 -0.05701946065013175 1107 9 -0.06270614625864113 1107 10-11 0.04320987512014085 1107 12-13 -0.01770348950035583 1107 14-15 0.025470146035836194 1107 16-17 -3.0007559966094277E-4 1107 18-19 -0.06896084928533952 1107 20-21 -0.10263467905224388 1107 22-23 -0.05603251590041225 1107 24-25 -0.16130645052254522 1107 26-27 -0.0618640217718891 1107 28-29 -0.1271528451194328 1107 30-31 -0.05086539440221571 1107 32-33 0.040923321764196885 1107 34-35 -0.039046580341484116 1107 36-37 -0.003522675118020402 1107 38-39 -0.0901698754203295 1107 40-41 -0.20574730613149228 1107 42-43 -0.1396618095727149 1107 44-45 -0.1417245654562791 1107 46-47 -0.26837136988330457 1107 48-49 -0.10104094576008293 1107 50-51 -0.06194701227493482 1107 52-53 -0.0337225995490229 1107 54-55 -0.10765051326961128 1107 56-57 0.0015266210324966778 1107 58-59 -0.09722123377476777 1107 60-61 -0.13456909853896803 1107 62-63 -0.13070623749092647 1107 64-65 -0.20132186961658505 1107 66-67 -0.03553935632415062 1107 68-69 -0.12147885392737123 1107 70-71 -0.08682241434070903 1107 72-73 -0.155345282217489 1107 74-75 -0.1494266392939423 1107 76-77 -0.42819921961784857 1107 78-79 -0.09005081385370062 1107 80-81 0.09539338229329886 1107 82-83 0.0810525552705279 1107 84-85 0.08972358804747316 1107 86-87 0.09006349038177675 1107 88-89 0.23470520536391248 1107 90-91 0.3179180091842966 1107 92-93 0.2974744829514826 1107 94-95 0.2185533024458337 1107 96-97 0.04352599113900979 1107 98-99 -0.13767476926905076 1107 100-101 0.5119411646782943 1108 1 0.030904005947725466 1108 2 -0.025827791642477393 1108 3 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-3.6089247958469635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 13.0 5 53.0 6 140.0 7 399.0 8 918.0 9 1833.0 10 2936.0 11 4512.0 12 6246.0 13 8730.0 14 10536.0 15 13628.0 16 17624.0 17 24494.0 18 23915.0 19 18064.0 20 17290.0 21 18536.0 22 20482.0 23 22749.0 24 26317.0 25 31727.0 26 38102.0 27 46912.0 28 57157.0 29 70001.0 30 84139.0 31 103508.0 32 128188.0 33 163741.0 34 221369.0 35 318978.0 36 507540.0 37 775614.0 38 524081.0 39 34822.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88415114919018 19.284280689517107 14.444526816138275 23.38704134515444 2 15.78208391100844 21.051586910136173 38.524906241760846 24.641422937094543 3 20.197434853493224 24.002736969353478 29.808095283361226 25.99173289379208 4 13.450010253172653 16.480555142040817 36.59581515114014 33.47361945364639 5 13.872736816680655 37.362283905003096 34.179389039149996 14.585590239166255 6 32.4178415965535 35.89477315378623 16.604968274950036 15.082416974710236 7 28.402125246539477 32.49472544514612 20.814628019081063 18.28852128923333 8 26.078391632932828 34.46503847480619 19.581437393171207 19.87513249908977 9 25.88570372934494 17.177053459384584 20.65861837452239 36.27862443674809 10-11 25.471855190526664 25.859054448232836 26.93113873588844 21.73795162535206 12-13 26.494018783334024 23.31368191728948 27.297024547231253 22.895274752145244 14-15 23.074153378995828 24.827958528895582 26.162230899817025 25.93565719229156 16-17 22.434983102041073 27.851567001516937 26.640617608404092 23.072832288037894 18-19 22.393228003656468 27.340428648380744 28.08977713847403 22.176566209488758 20-21 23.424404224904986 25.90517877886208 27.834763097904947 22.835653898327983 22-23 22.96736083335874 25.781273820690842 28.11493178800385 23.136433557946567 24-25 23.359567106865562 25.971286333746452 27.940523228667274 22.72862333072072 26-27 22.951517738586542 25.69918865419187 28.716730248294176 22.632563358927406 28-29 22.51814931130366 26.822538805119773 28.147679165971663 22.511632717604908 30-31 22.96951310283723 25.94983837054069 27.770419208287674 23.310229318334407 32-33 22.921281341676963 26.075566779242315 28.068388960531564 22.934762918549158 34-35 23.553607834462305 26.011697287472874 27.83229503590197 22.60239984216285 36-37 23.24432980090459 25.73227132771678 28.375755910703397 22.647642960675228 38-39 22.8555630446101 26.368273435097777 28.146766933297933 22.62939658699419 40-41 23.068881795555356 26.461151643022113 27.66602854565206 22.803938015770477 42-43 22.734508828770768 26.411804436961937 27.365161318865404 23.488525415401888 44-45 23.113619912417146 25.893977187923493 27.536525613104633 23.45587728655472 46-47 23.60928952315274 26.49946768529118 27.296467531792036 22.594775259764045 48-49 23.37283806467919 26.393378402595523 27.264258120097313 22.969525412627974 50-51 22.488294726282078 26.741681655415515 27.81931714677993 22.950706471522473 52-53 23.205246395994866 27.06845053935275 26.610316066751444 23.11598699790094 54-55 22.841587585709647 26.500879142296697 26.983166861267698 23.674366410725955 56-57 23.11975346948829 26.443066792694005 27.035180040331134 23.401999697486566 58-59 23.712075659683876 26.223527936470788 26.905037238115437 23.159359165729903 60-61 23.179139051367965 26.597721463211503 27.544117466819046 22.679022018601483 62-63 22.736126381263777 26.563414527699408 27.522011439312276 23.178447651724536 64-65 22.540740789454805 27.08725924402504 27.153890119140428 23.218109847379722 66-67 22.935207870737393 26.48060594538169 26.681368100220848 23.902818083660065 68-69 22.545902508697427 26.511609623459233 27.188167552601577 23.754320315241767 70-71 23.337610968921826 26.275996874426433 26.820999334590017 23.565392822061717 72-73 23.06052121988243 26.129358569888673 27.258866602935576 23.551253607293322 74-75 22.44790162693637 27.243845555533635 26.727838948066335 23.58041386946366 76-77 22.781687590499843 26.2958799413139 26.755599691844107 24.16683277634215 78-79 22.82319760320629 26.416624505556673 26.96990780355439 23.790270087682654 80-81 23.17140793697198 26.27744666706125 26.676767386899737 23.874378009067033 82-83 22.923732537471906 26.092515901385404 27.24436867658743 23.739382884555255 84-85 22.636045850514122 26.42646143581484 26.94678752855494 23.990705185116102 86-87 22.438923262185973 26.47054553129259 26.94005973893591 24.150471467585525 88-89 22.528510474370638 26.392615613364594 26.543974881747758 24.534899030517014 90-91 22.73590784373568 25.84825141107156 26.73698465986933 24.67885608532343 92-93 22.997701854479082 26.21257965639018 26.674764580579474 24.114953908551264 94-95 22.776615950827818 25.95155719686032 26.837858778838168 24.433968073473697 96-97 22.070700639227475 25.885947637393254 27.39009316985563 24.653258553523642 98-99 22.418711472134387 26.224013587994644 26.506289708624564 24.85098523124641 100-101 22.431104346717458 25.310551881157252 27.14823762153078 25.110106150594515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 698.0 1 510.0 2 372.5 3 465.5 4 436.5 5 464.0 6 796.0 7 1482.5 8 2364.0 9 2840.5 10 2645.0 11 2445.0 12 2636.0 13 2890.0 14 3132.5 15 3328.5 16 3539.5 17 3824.5 18 4196.5 19 4577.0 20 4983.5 21 5726.0 22 6914.0 23 8502.0 24 10244.5 25 12209.5 26 15357.0 27 19095.0 28 22484.0 29 26283.0 30 29815.5 31 34370.5 32 39811.5 33 45379.5 34 51572.5 35 56959.0 36 64203.5 37 71423.0 38 76211.0 39 80392.5 40 83698.5 41 86136.0 42 89216.5 43 95223.5 44 101975.0 45 107860.0 46 114621.5 47 136140.0 48 169590.5 49 167227.0 50 139987.0 51 129961.0 52 128429.0 53 128040.0 54 126653.0 55 121284.5 56 112088.0 57 101340.5 58 93511.5 59 85658.0 60 72806.5 61 59042.0 62 47011.5 63 35970.0 64 26668.0 65 18994.5 66 12950.5 67 8913.0 68 6236.5 69 4588.0 70 3266.0 71 1947.5 72 1167.5 73 755.0 74 479.5 75 271.5 76 153.0 77 86.0 78 46.0 79 24.5 80 14.0 81 7.0 82 6.0 83 6.5 84 6.5 85 4.0 86 2.5 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40429784300748567 2 0.0 3 0.0 4 0.0 5 5.380673696217924E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.00110602737088924 16-17 0.0023764642158295834 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002570766321526342 36-37 0.007517996858882266 38-39 0.012689422133580605 40-41 0.0020326989519045494 42-43 6.426915803815855E-4 44-45 4.932284221533098E-4 46-47 3.1387263227937895E-4 48-49 3.1387263227937895E-4 50-51 5.679600012674476E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.1387263227937895E-4 60-61 0.002660444216463307 62-63 0.0 64-65 2.9892631645655137E-4 66-67 0.0016142021088653776 68-69 0.0019131284253219288 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12375549501301226 78-79 1.0462421075979297E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1476247613820679 88-89 0.4429938546727863 90-91 0.34088062497122834 92-93 0.0 94-95 0.0 96-97 5.380673696217924E-4 98-99 0.0 100-101 3.4376526392503406E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3345306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.226873446792446 #Duplication Level Percentage of deduplicated Percentage of total 1 67.07678664717422 27.65366194320549 2 15.322193669860901 12.633722787091992 3 6.308914508975784 7.802904601445319 4 3.2274935936287505 5.322378797394634 5 1.9559396097172321 4.031863737969046 6 1.3001803613525202 3.216142272929113 7 0.8705475452264055 2.5122967433525387 8 0.6448882469574633 2.1269380915711333 9 0.4744074454221579 1.7602502143171854 >10 2.580840961248986 19.05614834611701 >50 0.16753481756590677 4.725135416578766 >100 0.06628296675834709 4.513557476714244 >500 0.0019585437284555572 0.539045198267045 >1k 0.0013056958176556322 1.476669835192717 >5k 5.803092522913921E-4 1.6441401879222568 >10k+ 1.4507731307284802E-4 0.9851443499315178 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22377 0.6689074183348249 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10565 0.3158156533363465 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9379 0.28036299220459954 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8427 0.25190520687793583 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7747 0.23157821735889034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7631 0.22811067208799435 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6115 0.18279344251318114 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5324 0.15914837088146794 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5223 0.1561292150852568 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5132 0.15340898560550215 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4881 0.14590593506244273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4876 0.14575647190421442 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4734 0.1415117182105314 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4150 0.12405442132946883 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3530 0.10552098970916263 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.9785263291310274E-5 2 0.0 0.0 0.0 0.0 5.9785263291310274E-5 3 0.0 0.0 0.0 0.0 5.9785263291310274E-5 4 0.0 0.0 0.0 0.0 5.9785263291310274E-5 5 0.0 0.0 0.0 0.0 8.967789493696541E-5 6 0.0 0.0 0.0 0.0 1.7935578987393082E-4 7 0.0 0.0 0.0 0.0 1.7935578987393082E-4 8 0.0 0.0 0.0 0.0 1.7935578987393082E-4 9 0.0 0.0 0.0 0.0 1.7935578987393082E-4 10-11 0.0 0.0 0.0 0.0 2.0924842151958596E-4 12-13 0.0 0.0 0.0 0.0 6.277452645587578E-4 14-15 0.0 0.0 0.0 1.4946315822827569E-5 7.62262106964206E-4 16-17 0.0 0.0 0.0 7.473157911413784E-5 9.416178968381368E-4 18-19 0.0 0.0 0.0 1.7935578987393082E-4 0.0012405442132946882 20-21 0.0 0.0 0.0 2.989263164565513E-4 0.001345168424054481 22-23 0.0 0.0 0.0 4.633357905076546E-4 0.0014796852664599293 24-25 0.0 0.0 0.0 6.875305278500681E-4 0.0015245242139284119 26-27 0.0 0.0 0.0 0.0013750610557001362 0.0015693631613968946 28-29 0.0 0.0 0.0 0.004633357905076546 0.0015843094772197222 30-31 0.0 0.0 0.0 0.013840288451938328 0.0016142021088653773 32-33 0.0 0.0 0.0 0.03250823691464996 0.0017786115829164805 34-35 0.0 0.0 0.0 0.059127625395105854 0.0019131284253219288 36-37 0.0 0.0 0.0 0.10199365917497533 0.0019131284253219288 38-39 0.0 0.0 0.0 0.16482797089414242 0.0020028063202588943 40-41 0.0 0.0 0.0 0.23745211947726158 0.002017752636081722 42-43 0.0 0.0 0.0 0.3150982301768508 0.0020326989519045494 44-45 0.0 0.0 0.0 0.4097681945986406 0.0020775378993730317 46-47 0.0 0.0 0.0 0.5231210537989649 0.0021971084259556524 48-49 0.0 0.0 0.0 0.6392688740581579 0.002286786320892618 50-51 0.0 0.0 0.0 0.7714241985635992 0.0023316252683611006 52-53 0.0 0.0 0.0 0.9052834030728429 0.0025408736898806863 54-55 0.0 0.0 0.0 1.055404199197323 0.0027202294797546173 56-57 0.0 0.0 0.0 1.2401855017149404 0.0027800147430459277 58-59 0.0 0.0 0.0 1.4385231126838622 0.0028547463221600656 60-61 0.0 0.0 0.0 1.652195643687005 0.002989263164565514 62-63 0.0 0.0 0.0 1.8779896368224611 0.0031985115860850995 64-65 0.0 0.0 0.0 2.114963474193392 0.003213457901907927 66-67 0.0 0.0 0.0 2.39055859165051 0.0032284042177307547 68-69 0.0 0.0 0.0 2.7091243670982563 0.0032284042177307547 70-71 0.0 0.0 0.0 3.0467317489042856 0.0032433505335535823 72-73 0.0 0.0 0.0 3.39451757178566 0.0035871157974786162 74-75 0.0 0.0 0.0 3.76077704102405 0.003736578955706892 76-77 0.0 0.0 0.0 4.14385111556312 0.003766471587352547 78-79 0.0 0.0 0.0 4.604825388170768 0.003781417903175375 80-81 0.0 0.0 0.0 5.111341085090571 0.003841203166466685 82-83 0.0 0.0 0.0 5.613268263052767 0.004065397903809099 84-85 0.0 0.0 0.0 6.123415914717517 0.0041700221145688915 86-87 0.0 0.0 0.0 6.659316068545 0.004259700009505856 88-89 0.0 0.0 0.0 7.248664247754914 0.0043493779044428225 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 675 0.0 28.82325 3 GTACATG 10025 0.0 22.57096 1 TACATGG 9955 0.0 22.117682 2 GAGTACT 9475 0.0 20.960665 12-13 AGAGTAC 14445 0.0 19.859467 10-11 ACATGGG 10570 0.0 19.618658 3 GTACTTT 10130 0.0 19.300858 14-15 GAGTACA 8595 0.0 18.844032 1 AGTACTT 10220 0.0 18.48081 12-13 AGTACAT 8040 0.0 18.355543 2 CTTTGCG 1170 0.0 17.439959 2 GTGTAGC 1905 0.0 17.004143 1 ACTTTTT 11865 0.0 16.417791 16-17 CATGGGG 7285 0.0 16.414734 4 TACTTTT 11655 0.0 15.8593 14-15 GTATTAG 825 0.0 15.012749 1 TTGCGTT 1270 0.0 14.945798 4 TCTAGAC 1480 0.0 14.7488785 3 TAGGACC 1045 0.0 14.531035 4 CGTACAC 360 6.175942E-7 14.4995 3 >>END_MODULE