FastQCFastQC Report
Fri 27 May 2016
SRR939029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003647
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114960.38273472215609894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96860.32247464498990724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78910.2627139607284078No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA78450.2611824891540184No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA68360.22758999309839006No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61640.20521719096817967No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47200.1571423006764776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.15184873588674036No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT44410.14785359264920278No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41950.13966354901225078No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34030.11329560364450282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT86850.021.66678612-13
GTACATG136700.020.8334751
TACATGG139650.020.1450372
TATCACG2151.1107204E-619.8256132
GTACTTT95450.019.6160814-15
AGTACTT91450.019.51573212-13
ACATGGG137450.019.3993073
TTTGCGT7100.018.677653
CATGGGG58000.017.882934
GTAAGCG3100.016.87871794-95
CTTTGCG8250.016.6481762
ACTTTTT119000.015.89519216-17
ATAAGCG1800.002671571715.7870623
AGTACAT107800.015.4648782
TACTTTT114150.015.40722914-15
GTGTAGC16900.015.1665241
CATGGGA83050.015.0552124
GTATAGT7900.015.0207611
TATAGTA11100.014.9337072
ATACCGT2256.9411914E-414.7277196
Produced by FastQC (version 0.11.5)